                         SEQUENCE LISTING

<110>  Academia Sinica
       Ming-Che Shih 
 
<120>  Probing the pseudaminic acid-coated pathogenic bacteria with 
       bacteriophage tail-spike protein to guide the glycol-based 
       antibody with cross-species antimicrobial activity

<130>  5025-0350PWO1

<150>  US 63/011,424
<151>  2020-04-17

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  1695
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I-AB6TSP (WT) nucleic acid sequence

<400>  1
agtgaagctg ctcaagaggc tgctaatgca gcagaagtag ctgcttcaca aacacagtac       60

tacttaaagt acttcaaccc tgaaatagtg taccctaaaa atgcgcgcat tatgcttgat      120

aatggcgata ttgttcgctc taccgttgtg aataatacat ccaatccgaa tgttgatatg      180

actggatggg taaaggttag ttcggttagt cagatttttg atgaaactta caatatcacc      240

caatcagtaa ttaatggaaa tctaattact gttgataatt ttggcgcaaa gggtgatggt      300

gttacagatg atagcgcagc ttttcaagca tattgtgata gtgctttaac tggacaaaat      360

ctatatcttg gggcaaaagg tcgctacatc ctcaagaatc aagtagacct aaaaggtaaa      420

ggtttggttg gcaacgggtg cggaaaggtt agtgagttct actacaactt aggttgtatt      480

gacgttgatg gctcaagccc tgatcttcaa ggtaaaacgg cattcattaa ttgtggtcct      540

acaatccaaa atcttactgc aagatgctct aatggagcag ggaaacaggt ctcatttatt      600

gaaatagacg gctatctagc caatattgac cacataacgc ttattaattt ctacaatcag      660

attgttgtta aacaagcact tgttggtttt aacttcacaa acgcttggtt gtattactca      720

caaaatgctg gaatttattg tgaagaccca ttgaaccgag tcagcacaac tggaacattt      780

cacaacattt atttccaatt aggtgacggt catgcaatga ttttcgatag ggatgtccat      840

gggtgtgatt tcgataatat tatattcgaa tctatgaatg gaggtatcaa ggctcgaaca      900

gtagcacatt gcgggtttgg taaattctgg tgtcagaact taaagacagc tacatccaaa      960

gattggctag aggtcactgg tgctaatagc tgttatggga atagctttac tggctatgtt     1020

aaattattag gcgggtggac gtcgaaaaca tcaccaacgc tagactcttt gccaacaaat     1080

aactatggtg gtgtatcggt tagtgcagag ggcatctcta ttgttaatgc tggcaataaa     1140

gcaaaaatgc ttatgctgcc aagtggtttt aagactggca atgcaacgat tgatgaaacg     1200

cacattagct catctaccgt aacaccctta gttaagagac gtgttattgg tgcagatagc     1260

tctggagcac aataccttgc atcagacacc tatacaaagc tttctcgcaa gtggggaaca     1320

tataaccatg gatcaaataa tgctggggcg ttttatgcac caatgatgct tacttatgat     1380

cagtcattta gcacaccaca aaacaataat ggttggaaaa tcgttaaaga atctactggc     1440

gtttatcggg ttgaacgtgt ttctgggaat acttcggtaa tcacaaatgg acatattgtt     1500

gttggctcac cactaatggg ttctcgtctg ggtactggaa caggtgctac gcatggaatt     1560

caaatgattg agacctatgc aggttcgtgg acttcttaca cagaagcggc tggttttaaa     1620

gtgttttggc gcgattctag caatgcccta gtcgatcctc atagatttac agtcgcattt     1680

acagcaacga gttaa                                                      1695


<210>  2
<211>  564
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  I-AB6TSP (WT) amino acid sequence


<220>
<221>  VARIANT
<222>  (1)..(8)
<223>  Deletion in Trun-I-?AB6TSP (Truncation)

<220>
<221>  DOMAIN
<222>  (181)..(420)
<223>  Funactional domain

<220>
<221>  MUTAGEN
<222>  (312)..(312)
<223>  Catalytic residue mutation from Glu to Gln

<400>  2

Ser Glu Ala Ala Gln Glu Ala Ala Asn Ala Ala Glu Val Ala Ala Ser 
1               5                   10                  15      


Gln Thr Gln Tyr Tyr Leu Lys Tyr Phe Asn Pro Glu Ile Val Tyr Pro 
            20                  25                  30          


Lys Asn Ala Arg Ile Met Leu Asp Asn Gly Asp Ile Val Arg Ser Thr 
        35                  40                  45              


Val Val Asn Asn Thr Ser Asn Pro Asn Val Asp Met Thr Gly Trp Val 
    50                  55                  60                  


Lys Val Ser Ser Val Ser Gln Ile Phe Asp Glu Thr Tyr Asn Ile Thr 
65                  70                  75                  80  


Gln Ser Val Ile Asn Gly Asn Leu Ile Thr Val Asp Asn Phe Gly Ala 
                85                  90                  95      


Lys Gly Asp Gly Val Thr Asp Asp Ser Ala Ala Phe Gln Ala Tyr Cys 
            100                 105                 110         


Asp Ser Ala Leu Thr Gly Gln Asn Leu Tyr Leu Gly Ala Lys Gly Arg 
        115                 120                 125             


Tyr Ile Leu Lys Asn Gln Val Asp Leu Lys Gly Lys Gly Leu Val Gly 
    130                 135                 140                 


Asn Gly Cys Gly Lys Val Ser Glu Phe Tyr Tyr Asn Leu Gly Cys Ile 
145                 150                 155                 160 


Asp Val Asp Gly Ser Ser Pro Asp Leu Gln Gly Lys Thr Ala Phe Ile 
                165                 170                 175     


Asn Cys Gly Pro Thr Ile Gln Asn Leu Thr Ala Arg Cys Ser Asn Gly 
            180                 185                 190         


Ala Gly Lys Gln Val Ser Phe Ile Glu Ile Asp Gly Tyr Leu Ala Asn 
        195                 200                 205             


Ile Asp His Ile Thr Leu Ile Asn Phe Tyr Asn Gln Ile Val Val Lys 
    210                 215                 220                 


Gln Ala Leu Val Gly Phe Asn Phe Thr Asn Ala Trp Leu Tyr Tyr Ser 
225                 230                 235                 240 


Gln Asn Ala Gly Ile Tyr Cys Glu Asp Pro Leu Asn Arg Val Ser Thr 
                245                 250                 255     


Thr Gly Thr Phe His Asn Ile Tyr Phe Gln Leu Gly Asp Gly His Ala 
            260                 265                 270         


Met Ile Phe Asp Arg Asp Val His Gly Cys Asp Phe Asp Asn Ile Ile 
        275                 280                 285             


Phe Glu Ser Met Asn Gly Gly Ile Lys Ala Arg Thr Val Ala His Cys 
    290                 295                 300                 


Gly Phe Gly Lys Phe Trp Cys Gln Asn Leu Lys Thr Ala Thr Ser Lys 
305                 310                 315                 320 


Asp Trp Leu Glu Val Thr Gly Ala Asn Ser Cys Tyr Gly Asn Ser Phe 
                325                 330                 335     


Thr Gly Tyr Val Lys Leu Leu Gly Gly Trp Thr Ser Lys Thr Ser Pro 
            340                 345                 350         


Thr Leu Asp Ser Leu Pro Thr Asn Asn Tyr Gly Gly Val Ser Val Ser 
        355                 360                 365             


Ala Glu Gly Ile Ser Ile Val Asn Ala Gly Asn Lys Ala Lys Met Leu 
    370                 375                 380                 


Met Leu Pro Ser Gly Phe Lys Thr Gly Asn Ala Thr Ile Asp Glu Thr 
385                 390                 395                 400 


His Ile Ser Ser Ser Thr Val Thr Pro Leu Val Lys Arg Arg Val Ile 
                405                 410                 415     


Gly Ala Asp Ser Ser Gly Ala Gln Tyr Leu Ala Ser Asp Thr Tyr Thr 
            420                 425                 430         


Lys Leu Ser Arg Lys Trp Gly Thr Tyr Asn His Gly Ser Asn Asn Ala 
        435                 440                 445             


Gly Ala Phe Tyr Ala Pro Met Met Leu Thr Tyr Asp Gln Ser Phe Ser 
    450                 455                 460                 


Thr Pro Gln Asn Asn Asn Gly Trp Lys Ile Val Lys Glu Ser Thr Gly 
465                 470                 475                 480 


Val Tyr Arg Val Glu Arg Val Ser Gly Asn Thr Ser Val Ile Thr Asn 
                485                 490                 495     


Gly His Ile Val Val Gly Ser Pro Leu Met Gly Ser Arg Leu Gly Thr 
            500                 505                 510         


Gly Thr Gly Ala Thr His Gly Ile Gln Met Ile Glu Thr Tyr Ala Gly 
        515                 520                 525             


Ser Trp Thr Ser Tyr Thr Glu Ala Ala Gly Phe Lys Val Phe Trp Arg 
    530                 535                 540                 


Asp Ser Ser Asn Ala Leu Val Asp Pro His Arg Phe Thr Val Ala Phe 
545                 550                 555                 560 


Thr Ala Thr Ser 
                


<210>  3
<211>  1671
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trun-I-AB6TSP (Truncation) nucleic acid sequence

<400>  3
aatgcagcag aagtagctgc ttcacaaaca cagtactact taaagtactt caaccctgaa       60

atagtgtacc ctaaaaatgc gcgcattatg cttgataatg gcgatattgt tcgctctacc      120

gttgtgaata atacatccaa tccgaatgtt gatatgactg gatgggtaaa ggttagttcg      180

gttagtcaga tttttgatga aacttacaat atcacccaat cagtaattaa tggaaatcta      240

attactgttg ataattttgg cgcaaagggt gatggtgtta cagatgatag cgcagctttt      300

caagcatatt gtgatagtgc tttaactgga caaaatctat atcttggggc aaaaggtcgc      360

tacatcctca agaatcaagt agacctaaaa ggtaaaggtt tggttggcaa cgggtgcgga      420

aaggttagtg agttctacta caacttaggt tgtattgacg ttgatggctc aagccctgat      480

cttcaaggta aaacggcatt cattaattgt ggtcctacaa tccaaaatct tactgcaaga      540

tgctctaatg gagcagggaa acaggtctca tttattgaaa tagacggcta tctagccaat      600

attgaccaca taacgcttat taatttctac aatcagattg ttgttaaaca agcacttgtt      660

ggttttaact tcacaaacgc ttggttgtat tactcacaaa atgctggaat ttattgtgaa      720

gacccattga accgagtcag cacaactgga acatttcaca acatttattt ccaattaggt      780

gacggtcatg caatgatttt cgatagggat gtccatgggt gtgatttcga taatattata      840

ttcgaatcta tgaatggagg tatcaaggct cgaacagtag cacattgcgg gtttggtaaa      900

ttctggtgtc agaacttaaa gacagctaca tccaaagatt ggctagaggt cactggtgct      960

aatagctgtt atgggaatag ctttactggc tatgttaaat tattaggcgg gtggacgtcg     1020

aaaacatcac caacgctaga ctctttgcca acaaataact atggtggtgt atcggttagt     1080

gcagagggca tctctattgt taatgctggc aataaagcaa aaatgcttat gctgccaagt     1140

ggttttaaga ctggcaatgc aacgattgat gaaacgcaca ttagctcatc taccgtaaca     1200

cccttagtta agagacgtgt tattggtgca gatagctctg gagcacaata ccttgcatca     1260

gacacctata caaagctttc tcgcaagtgg ggaacatata accatggatc aaataatgct     1320

ggggcgtttt atgcaccaat gatgcttact tatgatcagt catttagcac accacaaaac     1380

aataatggtt ggaaaatcgt taaagaatct actggcgttt atcgggttga acgtgtttct     1440

gggaatactt cggtaatcac aaatggacat attgttgttg gctcaccact aatgggttct     1500

cgtctgggta ctggaacagg tgctacgcat ggaattcaaa tgattgagac ctatgcaggt     1560

tcgtggactt cttacacaga agcggctggt tttaaagtgt tttggcgcga ttctagcaat     1620

gccctagtcg atcctcatag atttacagtc gcatttacag caacgagtta a              1671


<210>  4
<211>  556
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Trun-I-AB6TSP (Truncation) amino acid sequence


<220>
<221>  DOMAIN
<222>  (173)..(412)
<223>  Funactional domain

<220>
<221>  MUTAGEN
<222>  (304)..(304)
<223>  Catalytic residue mutation from Glu to Gln

<400>  4

Asn Ala Ala Glu Val Ala Ala Ser Gln Thr Gln Tyr Tyr Leu Lys Tyr 
1               5                   10                  15      


Phe Asn Pro Glu Ile Val Tyr Pro Lys Asn Ala Arg Ile Met Leu Asp 
            20                  25                  30          


Asn Gly Asp Ile Val Arg Ser Thr Val Val Asn Asn Thr Ser Asn Pro 
        35                  40                  45              


Asn Val Asp Met Thr Gly Trp Val Lys Val Ser Ser Val Ser Gln Ile 
    50                  55                  60                  


Phe Asp Glu Thr Tyr Asn Ile Thr Gln Ser Val Ile Asn Gly Asn Leu 
65                  70                  75                  80  


Ile Thr Val Asp Asn Phe Gly Ala Lys Gly Asp Gly Val Thr Asp Asp 
                85                  90                  95      


Ser Ala Ala Phe Gln Ala Tyr Cys Asp Ser Ala Leu Thr Gly Gln Asn 
            100                 105                 110         


Leu Tyr Leu Gly Ala Lys Gly Arg Tyr Ile Leu Lys Asn Gln Val Asp 
        115                 120                 125             


Leu Lys Gly Lys Gly Leu Val Gly Asn Gly Cys Gly Lys Val Ser Glu 
    130                 135                 140                 


Phe Tyr Tyr Asn Leu Gly Cys Ile Asp Val Asp Gly Ser Ser Pro Asp 
145                 150                 155                 160 


Leu Gln Gly Lys Thr Ala Phe Ile Asn Cys Gly Pro Thr Ile Gln Asn 
                165                 170                 175     


Leu Thr Ala Arg Cys Ser Asn Gly Ala Gly Lys Gln Val Ser Phe Ile 
            180                 185                 190         


Glu Ile Asp Gly Tyr Leu Ala Asn Ile Asp His Ile Thr Leu Ile Asn 
        195                 200                 205             


Phe Tyr Asn Gln Ile Val Val Lys Gln Ala Leu Val Gly Phe Asn Phe 
    210                 215                 220                 


Thr Asn Ala Trp Leu Tyr Tyr Ser Gln Asn Ala Gly Ile Tyr Cys Glu 
225                 230                 235                 240 


Asp Pro Leu Asn Arg Val Ser Thr Thr Gly Thr Phe His Asn Ile Tyr 
                245                 250                 255     


Phe Gln Leu Gly Asp Gly His Ala Met Ile Phe Asp Arg Asp Val His 
            260                 265                 270         


Gly Cys Asp Phe Asp Asn Ile Ile Phe Glu Ser Met Asn Gly Gly Ile 
        275                 280                 285             


Lys Ala Arg Thr Val Ala His Cys Gly Phe Gly Lys Phe Trp Cys Gln 
    290                 295                 300                 


Asn Leu Lys Thr Ala Thr Ser Lys Asp Trp Leu Glu Val Thr Gly Ala 
305                 310                 315                 320 


Asn Ser Cys Tyr Gly Asn Ser Phe Thr Gly Tyr Val Lys Leu Leu Gly 
                325                 330                 335     


Gly Trp Thr Ser Lys Thr Ser Pro Thr Leu Asp Ser Leu Pro Thr Asn 
            340                 345                 350         


Asn Tyr Gly Gly Val Ser Val Ser Ala Glu Gly Ile Ser Ile Val Asn 
        355                 360                 365             


Ala Gly Asn Lys Ala Lys Met Leu Met Leu Pro Ser Gly Phe Lys Thr 
    370                 375                 380                 


Gly Asn Ala Thr Ile Asp Glu Thr His Ile Ser Ser Ser Thr Val Thr 
385                 390                 395                 400 


Pro Leu Val Lys Arg Arg Val Ile Gly Ala Asp Ser Ser Gly Ala Gln 
                405                 410                 415     


Tyr Leu Ala Ser Asp Thr Tyr Thr Lys Leu Ser Arg Lys Trp Gly Thr 
            420                 425                 430         


Tyr Asn His Gly Ser Asn Asn Ala Gly Ala Phe Tyr Ala Pro Met Met 
        435                 440                 445             


Leu Thr Tyr Asp Gln Ser Phe Ser Thr Pro Gln Asn Asn Asn Gly Trp 
    450                 455                 460                 


Lys Ile Val Lys Glu Ser Thr Gly Val Tyr Arg Val Glu Arg Val Ser 
465                 470                 475                 480 


Gly Asn Thr Ser Val Ile Thr Asn Gly His Ile Val Val Gly Ser Pro 
                485                 490                 495     


Leu Met Gly Ser Arg Leu Gly Thr Gly Thr Gly Ala Thr His Gly Ile 
            500                 505                 510         


Gln Met Ile Glu Thr Tyr Ala Gly Ser Trp Thr Ser Tyr Thr Glu Ala 
        515                 520                 525             


Ala Gly Phe Lys Val Phe Trp Arg Asp Ser Ser Asn Ala Leu Val Asp 
    530                 535                 540                 


Pro His Arg Phe Thr Val Ala Phe Thr Ala Thr Ser 
545                 550                 555     


