                         SEQUENCE LISTING

<110>  INSTITUT PASTEUR
 
<120>  METHODS AND PRODUCTS FOR SEROLOGICAL ANALYSIS OF SARS-COV-2 
       INFECTION

<130>  B379414PCT D41475

<150>  US63011368
<151>  2020-04-17

<150>  US63020063
<151>  2020-05-05

<160>  20    

<170>  PatentIn version 3.5

<210>  1
<211>  1273
<212>  PRT
<213>  SARS COV 2


<220>
<221>  MISC_FEATURE
<223>  Genbank: QHD43416.1

<400>  1

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  2
<211>  3872
<212>  DNA
<213>  SARS COV 2


<220>
<221>  misc_feature
<223>  nucleotides 21563 to 25384 of Genbank: MN908947.3

<400>  2
atgtttgttt ttcttgtttt attgccacta gtctctagtc agtgtgttaa tcttacaacc       60

agaactcaat taccccctgc atacactaat tctttcacac gtggtgttta ttaccctgac      120

aaagttttca gatcctcagt tttacattca actcaggact tgttcttacc tttcttttcc      180

aatgttactt ggttccatgc tatacatgtc tctgggacca atggtactaa gaggtttgat      240

aaccctgtcc taccatttaa tgatggtgtt tattttgctt ccactgagaa gtctaacata      300

ataagaggct ggatttttgg tactacttta gattcgaaga cccagtccct acttattgtt      360

aataacgcta ctaatgttgt tattaaagtc tgtgaatttc aattttgtaa tgatccattt      420

ttgggtgttt attaccacaa aaacaacaaa agttggatgg aaagtgagtt cagagtttat      480

tctagtgcga ataattgcac ttttgaatat gtctctcagc cttttcttat ggaccttgaa      540

ggaaaacagg gtaatttcaa aaatcttagg gaatttgtgt ttaagaatat tgatggttat      600

tttaaaatat attctaagca cacgcctatt aatttagtgc gtgatctccc tcagggtttt      660

tcggctttag aaccattggt agatttgcca ataggtatta acatcactag gtttcaaact      720

ttacttgctt tacatagaag ttatttgact cctggtgatt cttcttcagg ttggacagct      780

ggtgctgcag cttattatgt gggttatctt caacctagga cttttctatt aaaatataat      840

gaaaatggaa ccattacaga tgctgtagac tgtgcacttg accctctctc agaaacaaag      900

tgtacgttga aatccttcac tgtagaaaaa ggaatctatc aaacttctaa ctttagagtc      960

caaccaacag aatctattgt tagatttcct aatattacaa acttgtgccc ttttggtgaa     1020

gtttttaacg ccaccagatt tgcatctgtt tatgcttgga acaggaagag aatcagcaac     1080

tgtgttgctg attattctgt cctatataat tccgcatcat tttccacttt taagtgttat     1140

ggagtgtctc ctactaaatt aaatgatctc tgctttacta atgtctatgc agattcattt     1200

gtaattagag gtgatgaagt cagacaaatc gctccagggc aaactggaaa gattgctgat     1260

tataattata aattaccaga tgattttaca ggctgcgtta tagcttggaa ttctaacaat     1320

cttgattcta aggttggtgg taattataat tacctgtata gattgtttag gaagtctaat     1380

ctcaaacctt ttgagagaga tatttcaact gaaatctatc aggccggtag cacaccttgt     1440

aatggtgttg aaggttttaa ttgttacttt cctttacaat catatggttt ccaacccact     1500

aatggtgttg gttaccaacc atacagagta gtagtacttt cttttgaact tctacatgca     1560

ccagcaactg tttgtggacc taaaaagtct actaatttgg ttaaaaacaa atgtgtcaat     1620

ttcaacttca atggtttaac aggcacaggt gttcttactg agtctaacaa aaagtttctg     1680

cctttccaac aatttggcag agacattgct gacactactg atgctgtccg tgatccacag     1740

acacttgaga ttcttgacat tacaccatgt tcttttggtg gtgtcagtgt tataacacca     1800

ggaacaaata cttctaacca ggttgctgtt ctttatcagg atgttaactg cacagaagtc     1860

cctgttgcta ttcatgcaga tcaacttact cctacttggc gtgtttattc tacaggttct     1920

aatgtttttc aaacacgtgc aggctgttta ataggggctg aacatgtcaa caactcatat     1980

gagtgtgaca tacccattgg tgcaggtata tgcgctagtt atcagactca gactaattct     2040

cctcggcggg cacgtagtgt agctagtcaa tccatcattg cctacactat gtcacttggt     2100

gcagaaaatt cagttgctta ctctaataac tctattgcca tacccacaaa ttttactatt     2160

agtgttacca cagaaattct accagtgtct atgaccaaga catcagtaga ttgtacaatg     2220

tacatttgtg gtgattcaac tgaatgcagc aatcttttgt tgcaatatgg cagtttttgt     2280

acacaattaa accgtgcttt aactggaata gctgttgaac aagacaaaaa cacccaagaa     2340

gtttttgcac aagtcaaaca aatttacaaa acaccaccaa ttaaagattt tggtggtttt     2400

aatttttcac aaatattacc agatccatca aaaccaagca agaggtcatt tattgaagat     2460

ctacttttca acaaagtgac acttgcagat gctggcttca tcaaacaata tggtgattgc     2520

cttggtgata ttgctgctag agacctcatt tgtgcacaaa agtttaacgg ccttactgtt     2580

ttgccacctt tgctcacaga tgaaatgatt gctcaataca cttctgcact gttagcgggt     2640

acaatcactt ctggttggac ctttggtgca ggtgctgcat tacaaatacc atttgctatg     2700

caaatggctt ataggtttaa tggtattgga gttacacaga atgttctcta tgagaaccaa     2760

aaattgattg ccaaccaatt taatagtgct attggcaaaa ttcaagactc actttcttcc     2820

acagcaagtg cacttggaaa acttcaagat gtggtcaacc aaaatgcaca agctttaaac     2880

acgcttgtta aacaacttag ctccaatttt ggtgcaattt caagtgtttt aaatgatatc     2940

ctttcacgtc ttgacaaagt tgaggctgaa gtgcaaattg ataggttgat cacaggcaga     3000

cttcaaagtt tgcagacata tgtgactcaa caattaatta gagctgcaga aatcagagct     3060

tctgctaatc ttgctgctac taaaatgtca gagtgtgtac ttggacaatc aaaaagagtt     3120

gatttttgtg gaaagggcta tcatcttatg tccttccctc agtcagcacc tcatggtgta     3180

gtcttcttgc atgtgactta tgtccctgca caagaaaaga acttcacaac tgctcctgcc     3240

atttgtcatg atggaaaagc acactttcct cgtgaaggtg tctttgtttc aaatggcaca     3300

cactggtttg taacacaaag gaatttttat gaaccacaaa tcattactac agacaacaca     3360

tttgtgtctg gtaactgtga tgttgtaata ggaattgtca acaacacagt ttatgatcct     3420

ttgcaacctg aattagactc attcaaggag gagttagata aatattttaa gaatcataca     3480

tcaccagatg ttgatttagg tgacatctct ggcattaatg cttcagttgt aaacattcaa     3540

aaagaaattg accgcctcaa tgaggttgcc aagaatttaa atgaatctct catcgatctc     3600

caagaacttg gaaagtatga gcagtatata aaatggccat ggtacatttg gctaggtttt     3660

atagctggct tgattgccat agtaatggtg acaattatgc tttgctgtat gaccagttgc     3720

tgtagttgtc tcaagggctg ttgttcttgt ggatcctgct gcaaatttga tgaagacgac     3780

tctgagccag tgctcaaagg agtcaaatta cattacacat aaacgaactt atggatttgt     3840

ttatgagaat cttcacaatt ggaactgtaa ct                                   3872


<210>  3
<211>  20
<212>  PRT
<213>  homo sapiens


<220>
<221>  MISC_FEATURE
<223>  exogenous signal peptide coming from a human kappa light chain

<400>  3

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly 
            20  


<210>  4
<211>  3813
<212>  DNA
<213>  SARS COV 2


<220>
<221>  misc_feature
<223>  codon optimized nucleotide sequence encoding the variant 
       Spike_B.1.1.7

<400>  4
atgttcgtgt ttctggtgct gctgccactg gtgtccagtc agtgcgtgaa cctgaccaca       60

cgaacacagc tgccaccagc ctacaccaat agcttcaccc gcggagtgta ctaccccgac      120

aaggtgttcc gcagcagcgt gctgcatagc acccaggatc tgtttctgcc cttcttcagc      180

aacgtgacct ggttccacgc catctccggc accaatggca ccaagcgctt cgataatccc      240

gtgctgccct tcaacgatgg cgtgtacttt gccagcaccg agaagtccaa tatcatccgc      300

ggctggatct tcggcaccac actggatagc aagacccaga gcctgctgat cgtgaacaac      360

gccaccaacg tggtcatcaa agtgtgcgag ttccagttct gcaacgaccc cttcctgggc      420

gtctaccaca agaacaacaa gagctggatg gaaagcgagt tccgcgtgta cagcagcgcc      480

aacaactgca ccttcgagta cgtgtcccag ccattcctga tggacctgga aggcaagcag      540

ggcaacttca agaacctgcg cgagttcgtg ttcaagaaca tcgacggcta cttcaaaatc      600

tacagcaagc acaccccaat caacctcgtg cgcgatctgc cacagggatt cagtgctctg      660

gaacccctgg tggatctgcc catcggcatc aacattaccc gctttcagac actgctggcc      720

ctgcaccgca gttacttgac accaggcgat agcagcagtg gatggacagc tggtgccgcc      780

gcttactacg ttggatatct gcagccacgc acctttctgc tgaagtacaa cgagaacggc      840

accatcaccg acgccgtgga ttgtgctctc gatcccctga gcgagacaaa gtgcaccctg      900

aagtccttca ccgtcgagaa gggcatctac cagaccagca atttccgcgt gcagcccacc      960

gagagcatcg tgcgcttccc caatatcacc aatctgtgcc ccttcggcga ggtgttcaat     1020

gccacacgct ttgcctccgt gtacgcctgg aatcgcaagc gcattagcaa ctgcgtggcc     1080

gactactccg tgctgtacaa tagcgccagc ttcagcacct tcaagtgcta cggcgtgtca     1140

cccaccaagc tgaacgacct gtgcttcacc aatgtgtacg ccgacagctt cgtgatccgc     1200

ggagatgaag tgcgacagat tgccccaggc cagaccggca agatcgccga ctacaattac     1260

aagctgcccg acgacttcac cggctgcgtg atcgcctgga acagcaacaa cctggattcc     1320

aaagtcggcg gcaactacaa ctacctgtac cgcctgttcc gcaagagcaa tctgaagccc     1380

ttcgagcgcg acatcagcac cgaaatctac caggccggaa gcaccccatg caacggcgtg     1440

gaaggcttca actgctactt cccactgcag tcctacggat ttcagcccac atatggcgtg     1500

ggctaccagc catatcgagt ggtggtgctg agcttcgaac tgctgcatgc tccagctacc     1560

gtgtgcggcc ccaagaagag taccaacctg gtcaagaaca aatgcgtgaa cttcaacttc     1620

aacggcctga ccggaaccgg cgtgctgacc gagagtaaca agaagttcct gccattccag     1680

cagtttggcc gcgacattga cgatacaacc gatgccgttc gcgatcccca gaccttggag     1740

atcctggata ttaccccatg ctccttcggc ggcgtgtccg tgattacacc aggcaccaat     1800

accagcaacc aggtggccgt tctgtaccag ggcgtgaatt gcacagaggt gcccgtggcc     1860

attcacgccg atcaattgac accaacatgg cgcgtgtact ccaccggcag caatgtgttt     1920

caaacccgcg ctggatgcct gattggagcc gagcacgtga acaatagcta cgagtgcgat     1980

atccccatcg gagccggaat ctgcgcctcc tatcagaccc agaccaatag tcaccgacga     2040

gcccgaagtg tggccagcca gagcatcatt gcctatacca tgagcctggg cgccgagaat     2100

agcgtggcct actccaacaa cagcattgct atccccatca acttcaccat cagcgtgacc     2160

accgagatcc tgccagtgtc catgaccaag accagcgtgg actgcaccat gtacatctgc     2220

ggagatagca ccgagtgcag caacctgctg ctgcagtacg gaagtttctg cacccagctg     2280

aatcgcgccc tgacaggcat tgccgtggaa caggataaga acacccaaga ggtgttcgcc     2340

caagtgaagc aaatctacaa gaccccacca atcaaggatt tcggcggctt caatttcagc     2400

cagattctgc ccgatccaag caagcccagc aagcgcagct tcatcgagga cctgctgttc     2460

aacaaagtga cactggccga cgccggattc atcaagcagt atggcgattg cctgggcgat     2520

attgccgcac gcgatctgat ttgcgcccag aagtttaacg gactgaccgt cctgccacca     2580

ctgctgacag atgagatgat cgcccagtac acaagtgccc tgctggccgg aaccattacc     2640

agcggatgga catttggagc cggtgccgct ctgcagattc ccttcgctat gcagatggcc     2700

taccgcttca atggcattgg cgtgacccag aatgtgctgt acgagaacca gaagctgatc     2760

gccaaccagt tcaacagcgc catcggcaag attcaggaca gcctgagtag taccgccagc     2820

gctctgggaa agctgcagga tgtggtcaac cagaacgctc aggccctgaa caccctggtt     2880

aagcagctga gcagcaactt cggcgccatc agtagcgtgc tgaacgatat cctggcccgc     2940

ctggataagg tggaagccga ggtgcagatc gatcgcctga ttaccggacg cctgcagtcc     3000

ctgcagacct atgtgacaca gcagctgatc cgagccgccg agattcgagc tagtgctaat     3060

ctggccgcca ccaagatgag cgaatgtgtg ctgggacaga gcaagcgcgt ggacttttgc     3120

ggcaagggat accacctgat gagcttccca cagagtgctc cacacggcgt ggtgtttctg     3180

catgtgacct acgtgcccgc tcaagagaag aatttcacca ccgctccagc catctgccac     3240

gacggaaagg cccattttcc acgcgagggc gtgttcgtta gcaacggcac tcattggttc     3300

gtcacccagc gcaacttcta cgagccccag atcatcacca cccacaacac cttcgtcagc     3360

ggcaactgcg acgtcgtgat cggcattgtg aacaacaccg tgtacgatcc actgcagccc     3420

gagctggaca gcttcaaaga ggaactggac aagtacttta agaaccacac aagccccgac     3480

gtggacctgg gagacattag cggaatcaac gccagcgtgg tcaacatcca gaaagagatt     3540

gaccgcctga acgaggtggc caagaatctg aacgagagcc tgatcgacct gcaagaactg     3600

ggcaaatacg agcagtacat taagtggccc tggtacatct ggctgggctt cattgccgga     3660

ctgattgcca tcgtgatggt caccattatg ctgtgctgca tgaccagttg ctgcagctgc     3720

ctgaagggat gctgcagttg cggaagctgc tgcaagttcg acgaggatga tagcgagcca     3780

gtgctgaagg gcgtcaagct gcactacacc tga                                  3813


<210>  5
<211>  3813
<212>  DNA
<213>  SARS COV 2


<220>
<221>  misc_feature
<223>  codon optimized nucleotide sequence encoding variant 
       Spike_B.1.351

<400>  5
atgttcgtgt ttctggtgct gctgccactg gtgtccagtc agtgcgtgaa cttcaccaca       60

cgaacacagc tgccaccagc ctacaccaat agcttcaccc gcggagtgta ctaccccgac      120

aaggtgttcc gcagcagcgt gctgcatagc acccaggatc tgtttctgcc cttcttcagc      180

aacgtgacct ggttccacgc catccacgtg tccggcacca atggcaccaa gcgcttcgct      240

aatcccgtgc tgcccttcaa cgatggcgtg tactttgcca gcaccgagaa gtccaatatc      300

atccgcggct ggatcttcgg caccacactg gatagcaaga cccagagcct gctgatcgtg      360

aacaacgcca ccaacgtggt catcaaagtg tgcgagttcc agttctgcaa cgaccccttc      420

ctgggcgtct actaccacaa gaacaacaag agctggatgg aaagcgagtt ccgcgtgtac      480

agcagcgcca acaactgcac cttcgagtac gtgtcccagc cattcctgat ggacctggaa      540

ggcaagcagg gcaacttcaa gaacctgcgc gagttcgtgt tcaagaacat cgacggctac      600

ttcaaaatct acagcaagca caccccaatc aacctcgtgc gcggcctgcc acagggattc      660

agtgctctgg aacccctggt ggatctgccc atcggcatca acattacccg ctttcagaca      720

ctgcaccgca gttacttgac accaggcgat agcagcagtg gatggacagc tggtgccgcc      780

gcttactacg ttggatatct gcagccacgc acctttctgc tgaagtacaa cgagaacggc      840

accatcaccg acgccgtgga ttgtgctctc gatcccctga gcgagacaaa gtgcaccctg      900

aagtccttca ccgtcgagaa gggcatctac cagaccagca atttccgcgt gcagcccacc      960

gagagcatcg tgcgcttccc caatatcacc aatctgtgcc ccttcggcga ggtgttcaat     1020

gccacacgct ttgcctccgt gtacgcctgg aatcgcaagc gcattagcaa ctgcgtggcc     1080

gactactccg tgctgtacaa tagcgccagc ttcagcacct tcaagtgcta cggcgtgtca     1140

cccaccaagc tgaacgacct gtgcttcacc aatgtgtacg ccgacagctt cgtgatccgc     1200

ggagatgaag tgcgacagat tgccccaggc cagaccggca acatcgccga ctacaattac     1260

aagctgcccg acgacttcac cggctgcgtg atcgcctgga acagcaacaa cctggattcc     1320

aaagtcggcg gcaactacaa ctacctgtac cgcctgttcc gcaagagcaa tctgaagccc     1380

ttcgagcgcg acatcagcac cgaaatctac caggccggaa gcaccccatg caacggcgtg     1440

aaaggcttca actgctactt cccactgcag tcctacggat ttcagcccac atatggcgtg     1500

ggctaccagc catatcgagt ggtggtgctg agcttcgaac tgctgcatgc tccagctacc     1560

gtgtgcggcc ccaagaagag taccaacctg gtcaagaaca aatgcgtgaa cttcaacttc     1620

aacggcctga ccggaaccgg cgtgctgacc gagagtaaca agaagttcct gccattccag     1680

cagtttggcc gcgacattgc cgatacaacc gatgccgttc gcgatcccca gaccttggag     1740

atcctggata ttaccccatg ctccttcggc ggcgtgtccg tgattacacc aggcaccaat     1800

accagcaacc aggtggccgt tctgtaccag ggcgtgaatt gcacagaggt gcccgtggcc     1860

attcacgccg atcaattgac accaacatgg cgcgtgtact ccaccggcag caatgtgttt     1920

caaacccgcg ctggatgcct gattggagcc gagcacgtga acaatagcta cgagtgcgat     1980

atccccatcg gagccggaat ctgcgcctcc tatcagaccc agaccaatag tccacgacga     2040

gcccgaagtg tggccagcca gagcatcatt gcctatacca tgagcctggg cgtggagaat     2100

agcgtggcct actccaacaa cagcattgct atccccacca acttcaccat cagcgtgacc     2160

accgagatcc tgccagtgtc catgaccaag accagcgtgg actgcaccat gtacatctgc     2220

ggagatagca ccgagtgcag caacctgctg ctgcagtacg gaagtttctg cacccagctg     2280

aatcgcgccc tgacaggcat tgccgtggaa caggataaga acacccaaga ggtgttcgcc     2340

caagtgaagc aaatctacaa gaccccacca atcaaggatt tcggcggctt caatttcagc     2400

cagattctgc ccgatccaag caagcccagc aagcgcagct tcatcgagga cctgctgttc     2460

aacaaagtga cactggccga cgccggattc atcaagcagt atggcgattg cctgggcgat     2520

attgccgcac gcgatctgat ttgcgcccag aagtttaacg gactgaccgt cctgccacca     2580

ctgctgacag atgagatgat cgcccagtac acaagtgccc tgctggccgg aaccattacc     2640

agcggatgga catttggagc cggtgccgct ctgcagattc ccttcgctat gcagatggcc     2700

taccgcttca atggcattgg cgtgacccag aatgtgctgt acgagaacca gaagctgatc     2760

gccaaccagt tcaacagcgc catcggcaag attcaggaca gcctgagtag taccgccagc     2820

gctctgggaa agctgcagga tgtggtcaac cagaacgctc aggccctgaa caccctggtt     2880

aagcagctga gcagcaactt cggcgccatc agtagcgtgc tgaacgatat cctgagccgc     2940

ctggataagg tggaagccga ggtgcagatc gatcgcctga ttaccggacg cctgcagtcc     3000

ctgcagacct atgtgacaca gcagctgatc cgagccgccg agattcgagc tagtgctaat     3060

ctggccgcca ccaagatgag cgaatgtgtg ctgggacaga gcaagcgcgt ggacttttgc     3120

ggcaagggat accacctgat gagcttccca cagagtgctc cacacggcgt ggtgtttctg     3180

catgtgacct acgtgcccgc tcaagagaag aatttcacca ccgctccagc catctgccac     3240

gacggaaagg cccattttcc acgcgagggc gtgttcgtta gcaacggcac tcattggttc     3300

gtcacccagc gcaacttcta cgagccccag atcatcacca ccgacaacac cttcgtcagc     3360

ggcaactgcg acgtcgtgat cggcattgtg aacaacaccg tgtacgatcc actgcagccc     3420

gagctggaca gcttcaaaga ggaactggac aagtacttta agaaccacac aagccccgac     3480

gtggacctgg gagacattag cggaatcaac gccagcgtgg tcaacatcca gaaagagatt     3540

gaccgcctga acgaggtggc caagaatctg aacgagagcc tgatcgacct gcaagaactg     3600

ggcaaatacg agcagtacat taagtggccc tggtacatct ggctgggctt cattgccgga     3660

ctgattgcca tcgtgatggt caccattatg ctgtgctgca tgaccagttg ctgcagctgc     3720

ctgaagggat gctgcagttg cggaagctgc tgcaagttcg acgaggatga tagcgagcca     3780

gtgctgaagg gcgtcaagct gcactacacc tga                                  3813


<210>  6
<211>  6
<212>  PRT
<213>  artificial

<220>
<223>  linker

<400>  6

Gly Gly Gly Ser Gly Gly 
1               5       


<210>  7
<211>  3822
<212>  DNA
<213>  SARS COV 2


<220>
<221>  misc_feature
<223>  codon optimized nucleotide sequence encoding Spike B.1-B

<400>  7
atgttcgtgt ttctggtgct gctgccactg gtgtccagtc agtgcgtgaa cctgaccaca       60

cgaacacagc tgccaccagc ctacaccaat agcttcaccc gcggagtgta ctaccccgac      120

aaggtgttcc gcagcagcgt gctgcatagc acccaggatc tgtttctgcc cttcttcagc      180

aacgtgacct ggttccacgc catccacgtg tccggcacca atggcaccaa gcgcttcgat      240

aatcccgtgc tgcccttcaa cgatggcgtg tactttgcca gcaccgagaa gtccaatatc      300

atccgcggct ggatcttcgg caccacactg gatagcaaga cccagagcct gctgatcgtg      360

aacaacgcca ccaacgtggt catcaaagtg tgcgagttcc agttctgcaa cgaccccttc      420

ctgggcgtct actaccacaa gaacaacaag agctggatgg aaagcgagtt ccgcgtgtac      480

agcagcgcca acaactgcac cttcgagtac gtgtcccagc cattcctgat ggacctggaa      540

ggcaagcagg gcaacttcaa gaacctgcgc gagttcgtgt tcaagaacat cgacggctac      600

ttcaaaatct acagcaagca caccccaatc aacctcgtgc gcgatctgcc acagggattc      660

agtgctctgg aacccctggt ggatctgccc atcggcatca acattacccg ctttcagaca      720

ctgctggccc tgcaccgcag ttacttgaca ccaggcgata gcagcagtgg atggacagct      780

ggtgccgccg cttactacgt tggatatctg cagccacgca cctttctgct gaagtacaac      840

gagaacggca ccatcaccga cgccgtggat tgtgctctcg atcccctgag cgagacaaag      900

tgcaccctga agtccttcac cgtcgagaag ggcatctacc agaccagcaa tttccgcgtg      960

cagcccaccg agagcatcgt gcgcttcccc aatatcacca atctgtgccc cttcggcgag     1020

gtgttcaatg ccacacgctt tgcctccgtg tacgcctgga atcgcaagcg cattagcaac     1080

tgcgtggccg actactccgt gctgtacaat agcgccagct tcagcacctt caagtgctac     1140

ggcgtgtcac ccaccaagct gaacgacctg tgcttcacca atgtgtacgc cgacagcttc     1200

gtgatccgcg gagatgaagt gcgacagatt gccccaggcc agaccggcaa gatcgccgac     1260

tacaattaca agctgcccga cgacttcacc ggctgcgtga tcgcctggaa cagcaacaac     1320

ctggattcca aagtcggcgg caactacaac tacctgtacc gcctgttccg caagagcaat     1380

ctgaagccct tcgagcgcga catcagcacc gaaatctacc aggccggaag caccccatgc     1440

aacggcgtgg aaggcttcaa ctgctacttc ccactgcagt cctacggatt tcagcccaca     1500

aatggcgtgg gctaccagcc atatcgagtg gtggtgctga gcttcgaact gctgcatgct     1560

ccagctaccg tgtgcggccc caagaagagt accaacctgg tcaagaacaa atgcgtgaac     1620

ttcaacttca acggcctgac cggaaccggc gtgctgaccg agagtaacaa gaagttcctg     1680

ccattccagc agtttggccg cgacattgcc gatacaaccg atgccgttcg cgatccccag     1740

accttggaga tcctggatat taccccatgc tccttcggcg gcgtgtccgt gattacacca     1800

ggcaccaata ccagcaacca ggtggccgtt ctgtaccagg gcgtgaattg cacagaggtg     1860

cccgtggcca ttcacgccga tcaattgaca ccaacatggc gcgtgtactc caccggcagc     1920

aatgtgtttc aaacccgcgc tggatgcctg attggagccg agcacgtgaa caatagctac     1980

gagtgcgata tccccatcgg agccggaatc tgcgcctcct atcagaccca gaccaatagt     2040

ccacgacgag cccgaagtgt ggccagccag agcatcattg cctataccat gagcctgggc     2100

gccgagaata gcgtggccta ctccaacaac agcattgcta tccccaccaa cttcaccatc     2160

agcgtgacca ccgagatcct gccagtgtcc atgaccaaga ccagcgtgga ctgcaccatg     2220

tacatctgcg gagatagcac cgagtgcagc aacctgctgc tgcagtacgg aagtttctgc     2280

acccagctga atcgcgccct gacaggcatt gccgtggaac aggataagaa cacccaagag     2340

gtgttcgccc aagtgaagca aatctacaag accccaccaa tcaaggattt cggcggcttc     2400

aatttcagcc agattctgcc cgatccaagc aagcccagca agcgcagctt catcgaggac     2460

ctgctgttca acaaagtgac actggccgac gccggattca tcaagcagta tggcgattgc     2520

ctgggcgata ttgccgcacg cgatctgatt tgcgcccaga agtttaacgg actgaccgtc     2580

ctgccaccac tgctgacaga tgagatgatc gcccagtaca caagtgccct gctggccgga     2640

accattacca gcggatggac atttggagcc ggtgccgctc tgcagattcc cttcgctatg     2700

cagatggcct accgcttcaa tggcattggc gtgacccaga atgtgctgta cgagaaccag     2760

aagctgatcg ccaaccagtt caacagcgcc atcggcaaga ttcaggacag cctgagtagt     2820

accgccagcg ctctgggaaa gctgcaggat gtggtcaacc agaacgctca ggccctgaac     2880

accctggtta agcagctgag cagcaacttc ggcgccatca gtagcgtgct gaacgatatc     2940

ctgagccgcc tggataaggt ggaagccgag gtgcagatcg atcgcctgat taccggacgc     3000

ctgcagtccc tgcagaccta tgtgacacag cagctgatcc gagccgccga gattcgagct     3060

agtgctaatc tggccgccac caagatgagc gaatgtgtgc tgggacagag caagcgcgtg     3120

gacttttgcg gcaagggata ccacctgatg agcttcccac agagtgctcc acacggcgtg     3180

gtgtttctgc atgtgaccta cgtgcccgct caagagaaga atttcaccac cgctccagcc     3240

atctgccacg acggaaaggc ccattttcca cgcgagggcg tgttcgttag caacggcact     3300

cattggttcg tcacccagcg caacttctac gagccccaga tcatcaccac cgacaacacc     3360

ttcgtcagcg gcaactgcga cgtcgtgatc ggcattgtga acaacaccgt gtacgatcca     3420

ctgcagcccg agctggacag cttcaaagag gaactggaca agtactttaa gaaccacaca     3480

agccccgacg tggacctggg agacattagc ggaatcaacg ccagcgtggt caacatccag     3540

aaagagattg accgcctgaa cgaggtggcc aagaatctga acgagagcct gatcgacctg     3600

caagaactgg gcaaatacga gcagtacatt aagtggccct ggtacatctg gctgggcttc     3660

attgccggac tgattgccat cgtgatggtc accattatgc tgtgctgcat gaccagttgc     3720

tgcagctgcc tgaagggatg ctgcagttgc ggaagctgct gcaagttcga cgaggatgat     3780

agcgagccag tgctgaaggg cgtcaagctg cactacacct ga                        3822


<210>  8
<211>  3822
<212>  DNA
<213>  SARS COV 2


<220>
<221>  misc_feature
<223>  codon optimized nucleotide sequence encoding Spike P.1

<400>  8
atgttcgtgt ttctggtgct gctgccactg gtgtccagtc agtgcgtgaa cttcaccaac       60

cgaacacagc tgccatccgc ctacaccaat agcttcaccc gcggagtgta ctaccccgac      120

aaggtgttcc gcagcagcgt gctgcatagc acccaggatc tgtttctgcc cttcttcagc      180

aacgtgacct ggttccacgc catccacgtg tccggcacca atggcaccaa gcgcttcgat      240

aatcccgtgc tgcccttcaa cgatggcgtg tactttgcca gcaccgagaa gtccaatatc      300

atccgcggct ggatcttcgg caccacactg gatagcaaga cccagagcct gctgatcgtg      360

aacaacgcca ccaacgtggt catcaaagtg tgcgagttcc agttctgcaa ctaccccttc      420

ctgggcgtct actaccacaa gaacaacaag agctggatgg aaagcgagtt ccgcgtgtac      480

agcagcgcca acaactgcac cttcgagtac gtgtcccagc cattcctgat ggacctggaa      540

ggcaagcagg gcaacttcaa gaacctgtcc gagttcgtgt tcaagaacat cgacggctac      600

ttcaaaatct acagcaagca caccccaatc aacctcgtgc gcgatctgcc acagggattc      660

agtgctctgg aacccctggt ggatctgccc atcggcatca acattacccg ctttcagaca      720

ctgctggccc tgcaccgcag ttacttgaca ccaggcgata gcagcagtgg atggacagct      780

ggtgccgccg cttactacgt tggatatctg cagccacgca cctttctgct gaagtacaac      840

gagaacggca ccatcaccga cgccgtggat tgtgctctcg atcccctgag cgagacaaag      900

tgcaccctga agtccttcac cgtcgagaag ggcatctacc agaccagcaa tttccgcgtg      960

cagcccaccg agagcatcgt gcgcttcccc aatatcacca atctgtgccc cttcggcgag     1020

gtgttcaatg ccacacgctt tgcctccgtg tacgcctgga atcgcaagcg cattagcaac     1080

tgcgtggccg actactccgt gctgtacaat agcgccagct tcagcacctt caagtgctac     1140

ggcgtgtcac ccaccaagct gaacgacctg tgcttcacca atgtgtacgc cgacagcttc     1200

gtgatccgcg gagatgaagt gcgacagatt gccccaggcc agaccggcac catcgccgac     1260

tacaattaca agctgcccga cgacttcacc ggctgcgtga tcgcctggaa cagcaacaac     1320

ctggattcca aagtcggcgg caactacaac tacctgtacc gcctgttccg caagagcaat     1380

ctgaagccct tcgagcgcga catcagcacc gaaatctacc aggccggaag caccccatgc     1440

aacggcgtga aaggcttcaa ctgctacttc ccactgcagt cctacggatt tcagcccaca     1500

tatggcgtgg gctaccagcc atatcgagtg gtggtgctga gcttcgaact gctgcatgct     1560

ccagctaccg tgtgcggccc caagaagagt accaacctgg tcaagaacaa atgcgtgaac     1620

ttcaacttca acggcctgac cggaaccggc gtgctgaccg agagtaacaa gaagttcctg     1680

ccattccagc agtttggccg cgacattgcc gatacaaccg atgccgttcg cgatccccag     1740

accttggaga tcctggatat taccccatgc tccttcggcg gcgtgtccgt gattacacca     1800

ggcaccaata ccagcaacca ggtggccgtt ctgtaccagg gcgtgaattg cacagaggtg     1860

cccgtggcca ttcacgccga tcaattgaca ccaacatggc gcgtgtactc caccggcagc     1920

aatgtgtttc aaacccgcgc tggatgcctg attggagccg agtacgtgaa caatagctac     1980

gagtgcgata tccccatcgg agccggaatc tgcgcctcct atcagaccca gaccaatagt     2040

ccacgacgag cccgaagtgt ggccagccag agcatcattg cctataccat gagcctgggc     2100

gccgagaata gcgtggccta ctccaacaac agcattgcta tccccaccaa cttcaccatc     2160

agcgtgacca ccgagatcct gccagtgtcc atgaccaaga ccagcgtgga ctgcaccatg     2220

tacatctgcg gagatagcac cgagtgcagc aacctgctgc tgcagtacgg aagtttctgc     2280

acccagctga atcgcgccct gacaggcatt gccgtggaac aggataagaa cacccaagag     2340

gtgttcgccc aagtgaagca aatctacaag accccaccaa tcaaggattt cggcggcttc     2400

aatttcagcc agattctgcc cgatccaagc aagcccagca agcgcagctt catcgaggac     2460

ctgctgttca acaaagtgac actggccgac gccggattca tcaagcagta tggcgattgc     2520

ctgggcgata ttgccgcacg cgatctgatt tgcgcccaga agtttaacgg actgaccgtc     2580

ctgccaccac tgctgacaga tgagatgatc gcccagtaca caagtgccct gctggccgga     2640

accattacca gcggatggac atttggagcc ggtgccgctc tgcagattcc cttcgctatg     2700

cagatggcct accgcttcaa tggcattggc gtgacccaga atgtgctgta cgagaaccag     2760

aagctgatcg ccaaccagtt caacagcgcc atcggcaaga ttcaggacag cctgagtagt     2820

accgccagcg ctctgggaaa gctgcaggat gtggtcaacc agaacgctca ggccctgaac     2880

accctggtta agcagctgag cagcaacttc ggcgccatca gtagcgtgct gaacgatatc     2940

ctgagccgcc tggataaggt ggaagccgag gtgcagatcg atcgcctgat taccggacgc     3000

ctgcagtccc tgcagaccta tgtgacacag cagctgatcc gagccgccga gattcgagct     3060

agtgctaatc tggccgccat caagatgagc gaatgtgtgc tgggacagag caagcgcgtg     3120

gacttttgcg gcaagggata ccacctgatg agcttcccac agagtgctcc acacggcgtg     3180

gtgtttctgc atgtgaccta cgtgcccgct caagagaaga atttcaccac cgctccagcc     3240

atctgccacg acggaaaggc ccattttcca cgcgagggcg tgttcgttag caacggcact     3300

cattggttcg tcacccagcg caacttctac gagccccaga tcatcaccac cgacaacacc     3360

ttcgtcagcg gcaactgcga cgtcgtgatc ggcattgtga acaacaccgt gtacgatcca     3420

ctgcagcccg agctggacag cttcaaagag gaactggaca agtactttaa gaaccacaca     3480

agccccgacg tggacctggg agacattagc ggaatcaacg ccagcgtggt caacatccag     3540

aaagagattg accgcctgaa cgaggtggcc aagaatctga acgagagcct gatcgacctg     3600

caagaactgg gcaaatacga gcagtacatt aagtggccct ggtacatctg gctgggcttc     3660

attgccggac tgattgccat cgtgatggtc accattatgc tgtgctgcat gaccagttgc     3720

tgcagctgcc tgaagggatg ctgcagttgc ggaagctgct gcaagttcga cgaggatgat     3780

agcgagccag tgctgaaggg cgtcaagctg cactacacct ga                        3822


<210>  9
<211>  1273
<212>  PRT
<213>  SARS COV 2


<220>
<221>  MISC_FEATURE
<223>  Protein sequence of Spike B.1-B

<400>  9

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  10
<211>  1270
<212>  PRT
<213>  SARS COV 2


<220>
<221>  MISC_FEATURE
<223>  Protein sequence of Spike B.1.1.7

<400>  10

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp Asn Pro 
65                  70                  75                  80  


Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu Lys Ser 
                85                  90                  95      


Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser Lys Thr 
            100                 105                 110         


Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile Lys Val 
        115                 120                 125             


Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr His Lys 
    130                 135                 140                 


Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr Ser Ser Ala 
145                 150                 155                 160 


Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu Met Asp Leu 
                165                 170                 175     


Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe Val Phe Lys 
            180                 185                 190         


Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr Pro Ile Asn 
        195                 200                 205             


Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu Pro Leu Val 
    210                 215                 220                 


Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr Leu Leu Ala 
225                 230                 235                 240 


Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser Gly Trp Thr 
                245                 250                 255     


Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro Arg Thr Phe 
            260                 265                 270         


Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala Val Asp Cys 
        275                 280                 285             


Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys Ser Phe Thr 
    290                 295                 300                 


Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val Gln Pro Thr 
305                 310                 315                 320 


Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly 
                325                 330                 335     


Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg 
            340                 345                 350         


Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser 
        355                 360                 365             


Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu 
    370                 375                 380                 


Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg 
385                 390                 395                 400 


Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala 
                405                 410                 415     


Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala 
            420                 425                 430         


Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr 
        435                 440                 445             


Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp 
    450                 455                 460                 


Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val 
465                 470                 475                 480 


Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro 
                485                 490                 495     


Thr Tyr Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe 
            500                 505                 510         


Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Thr 
        515                 520                 525             


Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn Gly Leu Thr 
    530                 535                 540                 


Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu Pro Phe Gln 
545                 550                 555                 560 


Gln Phe Gly Arg Asp Ile Asp Asp Thr Thr Asp Ala Val Arg Asp Pro 
                565                 570                 575     


Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe Gly Gly Val 
            580                 585                 590         


Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val Ala Val Leu 
        595                 600                 605             


Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile His Ala Asp 
    610                 615                 620                 


Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser Asn Val Phe 
625                 630                 635                 640 


Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val Asn Asn Ser 
                645                 650                 655     


Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala Ser Tyr Gln 
            660                 665                 670         


Thr Gln Thr Asn Ser His Arg Arg Ala Arg Ser Val Ala Ser Gln Ser 
        675                 680                 685             


Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser Val Ala Tyr 
    690                 695                 700                 


Ser Asn Asn Ser Ile Ala Ile Pro Ile Asn Phe Thr Ile Ser Val Thr 
705                 710                 715                 720 


Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val Asp Cys Thr 
                725                 730                 735     


Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu Leu Leu Gln 
            740                 745                 750         


Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr Gly Ile Ala 
        755                 760                 765             


Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln Val Lys Gln 
    770                 775                 780                 


Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe Asn Phe Ser 
785                 790                 795                 800 


Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser Phe Ile Glu 
                805                 810                 815     


Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly Phe Ile Lys 
            820                 825                 830         


Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp Leu Ile Cys 
        835                 840                 845             


Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu Leu Thr Asp 
    850                 855                 860                 


Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly Thr Ile Thr 
865                 870                 875                 880 


Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro Phe Ala 
                885                 890                 895     


Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln Asn Val 
            900                 905                 910         


Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn Ser Ala Ile 
        915                 920                 925             


Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala Leu Gly Lys 
    930                 935                 940                 


Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn Thr Leu Val 
945                 950                 955                 960 


Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val Leu Asn Asp 
                965                 970                 975     


Ile Leu Ala Arg Leu Asp Lys Val Glu Ala Glu Val Gln Ile Asp Arg 
            980                 985                 990         


Leu Ile Thr Gly Arg Leu Gln Ser  Leu Gln Thr Tyr Val  Thr Gln Gln 
        995                 1000                 1005             


Leu Ile  Arg Ala Ala Glu Ile  Arg Ala Ser Ala Asn  Leu Ala Ala 
    1010                 1015                 1020             


Thr Lys  Met Ser Glu Cys Val  Leu Gly Gln Ser Lys  Arg Val Asp 
    1025                 1030                 1035             


Phe Cys  Gly Lys Gly Tyr His  Leu Met Ser Phe Pro  Gln Ser Ala 
    1040                 1045                 1050             


Pro His  Gly Val Val Phe Leu  His Val Thr Tyr Val  Pro Ala Gln 
    1055                 1060                 1065             


Glu Lys  Asn Phe Thr Thr Ala  Pro Ala Ile Cys His  Asp Gly Lys 
    1070                 1075                 1080             


Ala His  Phe Pro Arg Glu Gly  Val Phe Val Ser Asn  Gly Thr His 
    1085                 1090                 1095             


Trp Phe  Val Thr Gln Arg Asn  Phe Tyr Glu Pro Gln  Ile Ile Thr 
    1100                 1105                 1110             


Thr His  Asn Thr Phe Val Ser  Gly Asn Cys Asp Val  Val Ile Gly 
    1115                 1120                 1125             


Ile Val  Asn Asn Thr Val Tyr  Asp Pro Leu Gln Pro  Glu Leu Asp 
    1130                 1135                 1140             


Ser Phe  Lys Glu Glu Leu Asp  Lys Tyr Phe Lys Asn  His Thr Ser 
    1145                 1150                 1155             


Pro Asp  Val Asp Leu Gly Asp  Ile Ser Gly Ile Asn  Ala Ser Val 
    1160                 1165                 1170             


Val Asn  Ile Gln Lys Glu Ile  Asp Arg Leu Asn Glu  Val Ala Lys 
    1175                 1180                 1185             


Asn Leu  Asn Glu Ser Leu Ile  Asp Leu Gln Glu Leu  Gly Lys Tyr 
    1190                 1195                 1200             


Glu Gln  Tyr Ile Lys Trp Pro  Trp Tyr Ile Trp Leu  Gly Phe Ile 
    1205                 1210                 1215             


Ala Gly  Leu Ile Ala Ile Val  Met Val Thr Ile Met  Leu Cys Cys 
    1220                 1225                 1230             


Met Thr  Ser Cys Cys Ser Cys  Leu Lys Gly Cys Cys  Ser Cys Gly 
    1235                 1240                 1245             


Ser Cys  Cys Lys Phe Asp Glu  Asp Asp Ser Glu Pro  Val Leu Lys 
    1250                 1255                 1260             


Gly Val  Lys Leu His Tyr Thr  
    1265                 1270 


<210>  11
<211>  1270
<212>  PRT
<213>  SARS COV 2


<220>
<221>  MISC_FEATURE
<223>  Protein sequence of Spike_B.1.351

<400>  11

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Phe Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Ala 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Gly Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser Gly Trp Thr 
                245                 250                 255     


Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro Arg Thr Phe 
            260                 265                 270         


Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala Val Asp Cys 
        275                 280                 285             


Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys Ser Phe Thr 
    290                 295                 300                 


Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val Gln Pro Thr 
305                 310                 315                 320 


Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly 
                325                 330                 335     


Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg 
            340                 345                 350         


Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser 
        355                 360                 365             


Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu 
    370                 375                 380                 


Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg 
385                 390                 395                 400 


Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Asn Ile Ala 
                405                 410                 415     


Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala 
            420                 425                 430         


Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr 
        435                 440                 445             


Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp 
    450                 455                 460                 


Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val 
465                 470                 475                 480 


Lys Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro 
                485                 490                 495     


Thr Tyr Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe 
            500                 505                 510         


Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Thr 
        515                 520                 525             


Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn Gly Leu Thr 
    530                 535                 540                 


Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu Pro Phe Gln 
545                 550                 555                 560 


Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val Arg Asp Pro 
                565                 570                 575     


Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe Gly Gly Val 
            580                 585                 590         


Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val Ala Val Leu 
        595                 600                 605             


Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile His Ala Asp 
    610                 615                 620                 


Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser Asn Val Phe 
625                 630                 635                 640 


Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val Asn Asn Ser 
                645                 650                 655     


Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala Ser Tyr Gln 
            660                 665                 670         


Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala Ser Gln Ser 
        675                 680                 685             


Ile Ile Ala Tyr Thr Met Ser Leu Gly Val Glu Asn Ser Val Ala Tyr 
    690                 695                 700                 


Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile Ser Val Thr 
705                 710                 715                 720 


Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val Asp Cys Thr 
                725                 730                 735     


Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu Leu Leu Gln 
            740                 745                 750         


Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr Gly Ile Ala 
        755                 760                 765             


Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln Val Lys Gln 
    770                 775                 780                 


Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe Asn Phe Ser 
785                 790                 795                 800 


Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser Phe Ile Glu 
                805                 810                 815     


Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly Phe Ile Lys 
            820                 825                 830         


Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp Leu Ile Cys 
        835                 840                 845             


Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu Leu Thr Asp 
    850                 855                 860                 


Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly Thr Ile Thr 
865                 870                 875                 880 


Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro Phe Ala 
                885                 890                 895     


Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln Asn Val 
            900                 905                 910         


Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn Ser Ala Ile 
        915                 920                 925             


Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala Leu Gly Lys 
    930                 935                 940                 


Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn Thr Leu Val 
945                 950                 955                 960 


Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val Leu Asn Asp 
                965                 970                 975     


Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln Ile Asp Arg 
            980                 985                 990         


Leu Ile Thr Gly Arg Leu Gln Ser  Leu Gln Thr Tyr Val  Thr Gln Gln 
        995                 1000                 1005             


Leu Ile  Arg Ala Ala Glu Ile  Arg Ala Ser Ala Asn  Leu Ala Ala 
    1010                 1015                 1020             


Thr Lys  Met Ser Glu Cys Val  Leu Gly Gln Ser Lys  Arg Val Asp 
    1025                 1030                 1035             


Phe Cys  Gly Lys Gly Tyr His  Leu Met Ser Phe Pro  Gln Ser Ala 
    1040                 1045                 1050             


Pro His  Gly Val Val Phe Leu  His Val Thr Tyr Val  Pro Ala Gln 
    1055                 1060                 1065             


Glu Lys  Asn Phe Thr Thr Ala  Pro Ala Ile Cys His  Asp Gly Lys 
    1070                 1075                 1080             


Ala His  Phe Pro Arg Glu Gly  Val Phe Val Ser Asn  Gly Thr His 
    1085                 1090                 1095             


Trp Phe  Val Thr Gln Arg Asn  Phe Tyr Glu Pro Gln  Ile Ile Thr 
    1100                 1105                 1110             


Thr Asp  Asn Thr Phe Val Ser  Gly Asn Cys Asp Val  Val Ile Gly 
    1115                 1120                 1125             


Ile Val  Asn Asn Thr Val Tyr  Asp Pro Leu Gln Pro  Glu Leu Asp 
    1130                 1135                 1140             


Ser Phe  Lys Glu Glu Leu Asp  Lys Tyr Phe Lys Asn  His Thr Ser 
    1145                 1150                 1155             


Pro Asp  Val Asp Leu Gly Asp  Ile Ser Gly Ile Asn  Ala Ser Val 
    1160                 1165                 1170             


Val Asn  Ile Gln Lys Glu Ile  Asp Arg Leu Asn Glu  Val Ala Lys 
    1175                 1180                 1185             


Asn Leu  Asn Glu Ser Leu Ile  Asp Leu Gln Glu Leu  Gly Lys Tyr 
    1190                 1195                 1200             


Glu Gln  Tyr Ile Lys Trp Pro  Trp Tyr Ile Trp Leu  Gly Phe Ile 
    1205                 1210                 1215             


Ala Gly  Leu Ile Ala Ile Val  Met Val Thr Ile Met  Leu Cys Cys 
    1220                 1225                 1230             


Met Thr  Ser Cys Cys Ser Cys  Leu Lys Gly Cys Cys  Ser Cys Gly 
    1235                 1240                 1245             


Ser Cys  Cys Lys Phe Asp Glu  Asp Asp Ser Glu Pro  Val Leu Lys 
    1250                 1255                 1260             


Gly Val  Lys Leu His Tyr Thr  
    1265                 1270 


<210>  12
<211>  1273
<212>  PRT
<213>  SARS COV 2


<220>
<221>  MISC_FEATURE
<223>  protein sequence Spike P.1

<400>  12

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Phe Thr Asn Arg Thr Gln Leu Pro Ser Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Tyr Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Ser Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Thr Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Lys Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Tyr Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu Tyr Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Ile Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  13
<211>  12558
<212>  DNA
<213>  artificial

<220>
<223>  lentiviral vector pLV-EF1a-Spike_B.1-G_IRES-Puromycin

<400>  13
aattcctcga gggcggccgc tctagagtcg acgggccgcg gtaacaattg ttaactaact       60

taagctagca acggtttccc tctagcggga tcaattccgc cccccccccc taacgttact      120

ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata      180

ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt      240

cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa      300

gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag      360

cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca      420

cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc      480

aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat      540

tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta      600

aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgataa      660

taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc      720

cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc      780

ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct      840

cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc      900

ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag      960

cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa     1020

ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct     1080

gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt     1140

cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac     1200

cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc     1260

ctgacccggg cggggcgcgt ctggaacaat caacctctgg attacaaaat ttgtgaaaga     1320

ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg     1380

cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc     1440

tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc     1500

actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt     1560

tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt     1620

gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg     1680

aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg     1740

tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg     1800

ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt     1860

tgggccgcct ccccgcctgg aattaattct gcagtcgaga cctagaaaaa catggagcaa     1920

tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg     1980

aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg     2040

cagctgtaga tcttagccac tttttaaaag aaaagagggg actggaaggg ctaattcact     2100

cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg     2160

attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct     2220

acaagctagt accagttgag ccagataagg tagaagaggc caataaagga gagaacacca     2280

gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt     2340

ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact     2400

tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga     2460

ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg     2520

ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc     2580

taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg     2640

tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg     2700

tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca     2760

aagaaatgaa tatcagagag tgagaggcct tgacattgct agcgtttacc gtcgacctct     2820

agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc     2880

acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga     2940

gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg     3000

tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg     3060

cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg     3120

gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga     3180

aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg     3240

gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag     3300

aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc     3360

gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg     3420

ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt     3480

cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc     3540

ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc     3600

actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg     3660

tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca     3720

gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc     3780

ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat     3840

cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt     3900

ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt     3960

tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc     4020

agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc     4080

gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata     4140

ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg     4200

gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc     4260

cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct     4320

acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa     4380

cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt     4440

cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca     4500

ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac     4560

tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca     4620

atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt     4680

tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc     4740

actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca     4800

aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata     4860

ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc     4920

ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc     4980

cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac ttgtttattg cagcttataa     5040

tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca     5100

ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat     5160

aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa     5220

ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag     5280

aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta attcactccc     5340

aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt     5400

agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca     5460

agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct     5520

tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga     5580

ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca     5640

agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc     5700

gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt     5760

tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa     5820

ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt     5880

gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg     5940

aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc     6000

tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac     6060

tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga     6120

gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag     6180

ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat     6240

tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc     6300

tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa     6360

ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga     6420

tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggc cgctgatctt     6480

cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt     6540

agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag     6600

agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag     6660

cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat     6720

agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact     6780

cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa     6840

ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt     6900

gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg     6960

gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga     7020

atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag     7080

tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat     7140

agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt     7200

taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga     7260

caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt     7320

agtgaacgga tctcgacggt atcgccttta aaagaaaagg ggggattggg gggtacagtg     7380

caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac     7440

aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttatc     7500

gatgaggccc tttcgtcttc actcgaggtg cccgtcagtg ggcagagcgc acatcgccca     7560

cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc     7620

gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg     7680

gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg     7740

ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg     7800

gcccttgcgt gccttgaatt acttccacct ggctgcagta cgtgattctt gatcccgagc     7860

ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc     7920

gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc     7980

ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg     8040

ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg     8100

gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt     8160

cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg     8220

gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa     8280

ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg     8340

cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac     8400

aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg     8460

cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg     8520

gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc     8580

cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt     8640

tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag     8700

gatcccgtac ggccaccatg ttcgtgtttc tggtgctgct gccactggtg tccagtcagt     8760

gcgtgaacct gaccacacga acacagctgc caccagccta caccaatagc ttcacccgcg     8820

gagtgtacta ccccgacaag gtgttccgca gcagcgtgct gcatagcacc caggatctgt     8880

ttctgccctt cttcagcaac gtgacctggt tccacgccat ccacgtgtcc ggcaccaatg     8940

gcaccaagcg cttcgataat cccgtgctgc ccttcaacga tggcgtgtac tttgccagca     9000

ccgagaagtc caatatcatc cgcggctgga tcttcggcac cacactggat agcaagaccc     9060

agagcctgct gatcgtgaac aacgccacca acgtggtcat caaagtgtgc gagttccagt     9120

tctgcaacga ccccttcctg ggcgtctact accacaagaa caacaagagc tggatggaaa     9180

gcgagttccg cgtgtacagc agcgccaaca actgcacctt cgagtacgtg tcccagccat     9240

tcctgatgga cctggaaggc aagcagggca acttcaagaa cctgcgcgag ttcgtgttca     9300

agaacatcga cggctacttc aaaatctaca gcaagcacac cccaatcaac ctcgtgcgcg     9360

atctgccaca gggattcagt gctctggaac ccctggtgga tctgcccatc ggcatcaaca     9420

ttacccgctt tcagacactg ctggccctgc accgcagtta cttgacacca ggcgatagca     9480

gcagtggatg gacagctggt gccgccgctt actacgttgg atatctgcag ccacgcacct     9540

ttctgctgaa gtacaacgag aacggcacca tcaccgacgc cgtggattgt gctctcgatc     9600

ccctgagcga gacaaagtgc accctgaagt ccttcaccgt cgagaagggc atctaccaga     9660

ccagcaattt ccgcgtgcag cccaccgaga gcatcgtgcg cttccccaat atcaccaatc     9720

tgtgcccctt cggcgaggtg ttcaatgcca cacgctttgc ctccgtgtac gcctggaatc     9780

gcaagcgcat tagcaactgc gtggccgact actccgtgct gtacaatagc gccagcttca     9840

gcaccttcaa gtgctacggc gtgtcaccca ccaagctgaa cgacctgtgc ttcaccaatg     9900

tgtacgccga cagcttcgtg atccgcggag atgaagtgcg acagattgcc ccaggccaga     9960

ccggcaagat cgccgactac aattacaagc tgcccgacga cttcaccggc tgcgtgatcg    10020

cctggaacag caacaacctg gattccaaag tcggcggcaa ctacaactac ctgtaccgcc    10080

tgttccgcaa gagcaatctg aagcccttcg agcgcgacat cagcaccgaa atctaccagg    10140

ccggaagcac cccatgcaac ggcgtggaag gcttcaactg ctacttccca ctgcagtcct    10200

acggatttca gcccacaaat ggcgtgggct accagccata tcgagtggtg gtgctgagct    10260

tcgaactgct gcatgctcca gctaccgtgt gcggccccaa gaagagtacc aacctggtca    10320

agaacaaatg cgtgaacttc aacttcaacg gcctgaccgg aaccggcgtg ctgaccgaga    10380

gtaacaagaa gttcctgcca ttccagcagt ttggccgcga cattgccgat acaaccgatg    10440

ccgttcgcga tccccagacc ttggagatcc tggatattac cccatgctcc ttcggcggcg    10500

tgtccgtgat tacaccaggc accaatacca gcaaccaggt ggccgttctg taccaggatg    10560

tgaattgcac agaggtgccc gtggccattc acgccgatca attgacacca acatggcgcg    10620

tgtactccac cggcagcaat gtgtttcaaa cccgcgctgg atgcctgatt ggagccgagc    10680

acgtgaacaa tagctacgag tgcgatatcc ccatcggagc cggaatctgc gcctcctatc    10740

agacccagac caatagtcca cgacgagccc gaagtgtggc cagccagagc atcattgcct    10800

ataccatgag cctgggcgcc gagaatagcg tggcctactc caacaacagc attgctatcc    10860

ccaccaactt caccatcagc gtgaccaccg agatcctgcc agtgtccatg accaagacca    10920

gcgtggactg caccatgtac atctgcggag atagcaccga gtgcagcaac ctgctgctgc    10980

agtacggaag tttctgcacc cagctgaatc gcgccctgac aggcattgcc gtggaacagg    11040

ataagaacac ccaagaggtg ttcgcccaag tgaagcaaat ctacaagacc ccaccaatca    11100

aggatttcgg cggcttcaat ttcagccaga ttctgcccga tccaagcaag cccagcaagc    11160

gcagcttcat cgaggacctg ctgttcaaca aagtgacact ggccgacgcc ggattcatca    11220

agcagtatgg cgattgcctg ggcgatattg ccgcacgcga tctgatttgc gcccagaagt    11280

ttaacggact gaccgtcctg ccaccactgc tgacagatga gatgatcgcc cagtacacaa    11340

gtgccctgct ggccggaacc attaccagcg gatggacatt tggagccggt gccgctctgc    11400

agattccctt cgctatgcag atggcctacc gcttcaatgg cattggcgtg acccagaatg    11460

tgctgtacga gaaccagaag ctgatcgcca accagttcaa cagcgccatc ggcaagattc    11520

aggacagcct gagtagtacc gccagcgctc tgggaaagct gcaggatgtg gtcaaccaga    11580

acgctcaggc cctgaacacc ctggttaagc agctgagcag caacttcggc gccatcagta    11640

gcgtgctgaa cgatatcctg agccgcctgg ataaggtgga agccgaggtg cagatcgatc    11700

gcctgattac cggacgcctg cagtccctgc agacctatgt gacacagcag ctgatccgag    11760

ccgccgagat tcgagctagt gctaatctgg ccgccaccaa gatgagcgaa tgtgtgctgg    11820

gacagagcaa gcgcgtggac ttttgcggca agggatacca cctgatgagc ttcccacaga    11880

gtgctccaca cggcgtggtg tttctgcatg tgacctacgt gcccgctcaa gagaagaatt    11940

tcaccaccgc tccagccatc tgccacgacg gaaaggccca ttttccacgc gagggcgtgt    12000

tcgttagcaa cggcactcat tggttcgtca cccagcgcaa cttctacgag ccccagatca    12060

tcaccaccga caacaccttc gtcagcggca actgcgacgt cgtgatcggc attgtgaaca    12120

acaccgtgta cgatccactg cagcccgagc tggacagctt caaagaggaa ctggacaagt    12180

actttaagaa ccacacaagc cccgacgtgg acctgggaga cattagcgga atcaacgcca    12240

gcgtggtcaa catccagaaa gagattgacc gcctgaacga ggtggccaag aatctgaacg    12300

agagcctgat cgacctgcaa gaactgggca aatacgagca gtacattaag tggccctggt    12360

acatctggct gggcttcatt gccggactga ttgccatcgt gatggtcacc attatgctgt    12420

gctgcatgac cagttgctgc agctgcctga agggatgctg cagttgcgga agctgctgca    12480

agttcgacga ggatgatagc gagccagtgc tgaagggcgt caagctgcac tacacctgat    12540

aacgagcgcg cctcgagg                                                  12558


<210>  14
<211>  12984
<212>  DNA
<213>  artificial

<220>
<223>  lentiviral vector pLV-EF1a-Spike_B.1-G_IRES-Hygromycin

<400>  14
aattcctcga gggcggccgc tctagagtcg acgggccgcg gtaacaattg ttaactaact       60

taagctagca acggtttccc tctagcggga tcaattccgc cccccccccc taacgttact      120

ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata      180

ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt      240

cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa      300

gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag      360

cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca      420

cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc      480

aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat      540

tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta      600

aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgataa      660

taccatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt      720

cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt      780

cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa      840

agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga      900

cattggggaa tttagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac      960

gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat     1020

ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca     1080

aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt     1140

gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga     1200

tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt     1260

cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga     1320

ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt     1380

ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc     1440

gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt     1500

tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc     1560

cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga     1620

tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc     1680

aaaggaatag cccgggcggg gcgcgtctgg aacaatcaac ctctggatta caaaatttgt     1740

gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct     1800

ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat     1860

aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg     1920

gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag     1980

ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc     2040

tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg     2100

tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc     2160

gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc     2220

ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc     2280

tccctttggg ccgcctcccc gcctggaatt aattctgcag tcgagaccta gaaaaacatg     2340

gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac     2400

aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt     2460

acaaggcagc tgtagatctt agccactttt taaaagaaaa gaggggactg gaagggctaa     2520

ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact     2580

tccctgatta gcagaactac acaccagggc caggggtcag atatccactg acctttggat     2640

ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga     2700

acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt     2760

tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg     2820

agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc     2880

cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag     2940

cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc     3000

tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa     3060

gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag     3120

tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa     3180

cttgcaaaga aatgaatatc agagagtgag aggccttgac attgctagcg tttaccgtcg     3240

acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat     3300

ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc     3360

taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga     3420

aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt     3480

attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg     3540

cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac     3600

gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg     3660

ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca     3720

agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc     3780

tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc     3840

ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag     3900

gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc     3960

ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca     4020

gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg     4080

aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg     4140

aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct     4200

ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa     4260

gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa     4320

gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa     4380

tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc     4440

ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga     4500

ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca     4560

atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc     4620

ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat     4680

tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc     4740

attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt     4800

tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc     4860

ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg     4920

gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt     4980

gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg     5040

gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga     5100

aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg     5160

taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg     5220

tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt     5280

tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc     5340

atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca     5400

tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcaacttgt ttattgcagc     5460

ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc     5520

actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa     5580

ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac     5640

tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt     5700

ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttggaa gggctaattc     5760

actcccaaag aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc     5820

ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt     5880

gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca     5940

ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag     6000

agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt     6060

acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag     6120

ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag     6180

ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct     6240

ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta     6300

gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca     6360

gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag     6420

aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc     6480

ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt     6540

gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa     6600

ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag     6660

aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg     6720

gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag     6780

tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag     6840

acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccggccgct     6900

gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata     6960

taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt     7020

gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc     7080

aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc     7140

tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt     7200

gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata     7260

cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac     7320

tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac     7380

gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat     7440

tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg     7500

ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat     7560

aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa     7620

tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg     7680

acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat     7740

tcgattagtg aacggatctc gacggtatcg cctttaaaag aaaagggggg attggggggt     7800

acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac     7860

aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag     7920

tttatcgatg aggccctttc gtcttcactc gaggtgcccg tcagtgggca gagcgcacat     7980

cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa     8040

ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg     8100

gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt     8160

ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg     8220

gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc     8280

ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt     8340

cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt     8400

ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat     8460

gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc     8520

acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca     8580

catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc     8640

aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg     8700

cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc     8760

ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac     8820

ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt     8880

accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag     8940

gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag     9000

ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat     9060

cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt     9120

cgtgaggatc ccgtacggcc accatgttcg tgtttctggt gctgctgcca ctggtgtcca     9180

gtcagtgcgt gaacctgacc acacgaacac agctgccacc agcctacacc aatagcttca     9240

cccgcggagt gtactacccc gacaaggtgt tccgcagcag cgtgctgcat agcacccagg     9300

atctgtttct gcccttcttc agcaacgtga cctggttcca cgccatccac gtgtccggca     9360

ccaatggcac caagcgcttc gataatcccg tgctgccctt caacgatggc gtgtactttg     9420

ccagcaccga gaagtccaat atcatccgcg gctggatctt cggcaccaca ctggatagca     9480

agacccagag cctgctgatc gtgaacaacg ccaccaacgt ggtcatcaaa gtgtgcgagt     9540

tccagttctg caacgacccc ttcctgggcg tctactacca caagaacaac aagagctgga     9600

tggaaagcga gttccgcgtg tacagcagcg ccaacaactg caccttcgag tacgtgtccc     9660

agccattcct gatggacctg gaaggcaagc agggcaactt caagaacctg cgcgagttcg     9720

tgttcaagaa catcgacggc tacttcaaaa tctacagcaa gcacacccca atcaacctcg     9780

tgcgcgatct gccacaggga ttcagtgctc tggaacccct ggtggatctg cccatcggca     9840

tcaacattac ccgctttcag acactgctgg ccctgcaccg cagttacttg acaccaggcg     9900

atagcagcag tggatggaca gctggtgccg ccgcttacta cgttggatat ctgcagccac     9960

gcacctttct gctgaagtac aacgagaacg gcaccatcac cgacgccgtg gattgtgctc    10020

tcgatcccct gagcgagaca aagtgcaccc tgaagtcctt caccgtcgag aagggcatct    10080

accagaccag caatttccgc gtgcagccca ccgagagcat cgtgcgcttc cccaatatca    10140

ccaatctgtg ccccttcggc gaggtgttca atgccacacg ctttgcctcc gtgtacgcct    10200

ggaatcgcaa gcgcattagc aactgcgtgg ccgactactc cgtgctgtac aatagcgcca    10260

gcttcagcac cttcaagtgc tacggcgtgt cacccaccaa gctgaacgac ctgtgcttca    10320

ccaatgtgta cgccgacagc ttcgtgatcc gcggagatga agtgcgacag attgccccag    10380

gccagaccgg caagatcgcc gactacaatt acaagctgcc cgacgacttc accggctgcg    10440

tgatcgcctg gaacagcaac aacctggatt ccaaagtcgg cggcaactac aactacctgt    10500

accgcctgtt ccgcaagagc aatctgaagc ccttcgagcg cgacatcagc accgaaatct    10560

accaggccgg aagcacccca tgcaacggcg tggaaggctt caactgctac ttcccactgc    10620

agtcctacgg atttcagccc acaaatggcg tgggctacca gccatatcga gtggtggtgc    10680

tgagcttcga actgctgcat gctccagcta ccgtgtgcgg ccccaagaag agtaccaacc    10740

tggtcaagaa caaatgcgtg aacttcaact tcaacggcct gaccggaacc ggcgtgctga    10800

ccgagagtaa caagaagttc ctgccattcc agcagtttgg ccgcgacatt gccgatacaa    10860

ccgatgccgt tcgcgatccc cagaccttgg agatcctgga tattacccca tgctccttcg    10920

gcggcgtgtc cgtgattaca ccaggcacca ataccagcaa ccaggtggcc gttctgtacc    10980

aggatgtgaa ttgcacagag gtgcccgtgg ccattcacgc cgatcaattg acaccaacat    11040

ggcgcgtgta ctccaccggc agcaatgtgt ttcaaacccg cgctggatgc ctgattggag    11100

ccgagcacgt gaacaatagc tacgagtgcg atatccccat cggagccgga atctgcgcct    11160

cctatcagac ccagaccaat agtccacgac gagcccgaag tgtggccagc cagagcatca    11220

ttgcctatac catgagcctg ggcgccgaga atagcgtggc ctactccaac aacagcattg    11280

ctatccccac caacttcacc atcagcgtga ccaccgagat cctgccagtg tccatgacca    11340

agaccagcgt ggactgcacc atgtacatct gcggagatag caccgagtgc agcaacctgc    11400

tgctgcagta cggaagtttc tgcacccagc tgaatcgcgc cctgacaggc attgccgtgg    11460

aacaggataa gaacacccaa gaggtgttcg cccaagtgaa gcaaatctac aagaccccac    11520

caatcaagga tttcggcggc ttcaatttca gccagattct gcccgatcca agcaagccca    11580

gcaagcgcag cttcatcgag gacctgctgt tcaacaaagt gacactggcc gacgccggat    11640

tcatcaagca gtatggcgat tgcctgggcg atattgccgc acgcgatctg atttgcgccc    11700

agaagtttaa cggactgacc gtcctgccac cactgctgac agatgagatg atcgcccagt    11760

acacaagtgc cctgctggcc ggaaccatta ccagcggatg gacatttgga gccggtgccg    11820

ctctgcagat tcccttcgct atgcagatgg cctaccgctt caatggcatt ggcgtgaccc    11880

agaatgtgct gtacgagaac cagaagctga tcgccaacca gttcaacagc gccatcggca    11940

agattcagga cagcctgagt agtaccgcca gcgctctggg aaagctgcag gatgtggtca    12000

accagaacgc tcaggccctg aacaccctgg ttaagcagct gagcagcaac ttcggcgcca    12060

tcagtagcgt gctgaacgat atcctgagcc gcctggataa ggtggaagcc gaggtgcaga    12120

tcgatcgcct gattaccgga cgcctgcagt ccctgcagac ctatgtgaca cagcagctga    12180

tccgagccgc cgagattcga gctagtgcta atctggccgc caccaagatg agcgaatgtg    12240

tgctgggaca gagcaagcgc gtggactttt gcggcaaggg ataccacctg atgagcttcc    12300

cacagagtgc tccacacggc gtggtgtttc tgcatgtgac ctacgtgccc gctcaagaga    12360

agaatttcac caccgctcca gccatctgcc acgacggaaa ggcccatttt ccacgcgagg    12420

gcgtgttcgt tagcaacggc actcattggt tcgtcaccca gcgcaacttc tacgagcccc    12480

agatcatcac caccgacaac accttcgtca gcggcaactg cgacgtcgtg atcggcattg    12540

tgaacaacac cgtgtacgat ccactgcagc ccgagctgga cagcttcaaa gaggaactgg    12600

acaagtactt taagaaccac acaagccccg acgtggacct gggagacatt agcggaatca    12660

acgccagcgt ggtcaacatc cagaaagaga ttgaccgcct gaacgaggtg gccaagaatc    12720

tgaacgagag cctgatcgac ctgcaagaac tgggcaaata cgagcagtac attaagtggc    12780

cctggtacat ctggctgggc ttcattgccg gactgattgc catcgtgatg gtcaccatta    12840

tgctgtgctg catgaccagt tgctgcagct gcctgaaggg atgctgcagt tgcggaagct    12900

gctgcaagtt cgacgaggat gatagcgagc cagtgctgaa gggcgtcaag ctgcactaca    12960

cctgataacg agcgcgcctc gagg                                           12984


<210>  15
<211>  8667
<212>  DNA
<213>  artificial

<220>
<223>  expression plasmid phCMV_Spike_B.1-G

<400>  15
tcgagcggga tcaattcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg       60

tggccaatgc cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg      120

gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca      180

ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa      240

atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc      300

tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg      360

ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt      420

gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca      480

gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat      540

ccctcgacgg atcggccgca attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt      600

tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt      660

gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg      720

ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg      780

cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg      840

cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat      900

aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc      960

gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc     1020

tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga     1080

agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt     1140

ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg     1200

taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc     1260

gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg     1320

gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc     1380

ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg     1440

ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc     1500

gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct     1560

caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt     1620

taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa     1680

aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa     1740

tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc     1800

tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct     1860

gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca     1920

gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt     1980

aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt     2040

gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc     2100

ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcgggttag     2160

ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt     2220

tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac     2280

tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg     2340

cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat     2400

tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc     2460

gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc     2520

tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa     2580

atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg     2640

tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg     2700

cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg     2760

ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct     2820

tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt     2880

ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat     2940

ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca     3000

ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac     3060

gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta     3120

gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg     3180

tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg     3240

ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca     3300

gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta atacgactca     3360

ctatagggcg aattggagct ccaccgcggt ggcggccgct ctagagagct tggcccattg     3420

catacgttgt atccatatca taatatgtac atttatattg gctcatgtcc aacattaccg     3480

ccatgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt     3540

catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga     3600

ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca     3660

atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca     3720

gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg     3780

cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc     3840

tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt     3900

ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt     3960

ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg     4020

acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg     4080

aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg     4140

gaccgatcca gcctccggtc gaccgatcct gagaacttca gggtgagttt ggggaccctt     4200

gattgttctt tctttttcgc tattgtaaaa ttcatgttat atggaggggg caaagttttc     4260

agggtgttgt ttagaatggg aagatgtccc ttgtatcacc atggaccctc atgataattt     4320

tgtttctttc actttctact ctgttgacaa ccattgtctc ctcttatttt cttttcattt     4380

tctgtaactt tttcgttaaa ctttagcttg catttgtaac gaatttttaa attcactttt     4440

gtttatttgt cagattgtaa gtactttctc taatcacttt tttttcaagg caatcagggt     4500

atattatatt gtacttcagc acagttttag agaacaattg ttataattaa atgataaggt     4560

agaatatttc tgcatataaa ttctggctgg cgtggaaata ttcttattgg tagaaacaac     4620

tacaccctgg tcatcatcct gcctttctct ttatggttac aatgatatac actgtttgag     4680

atgaggataa aatactctga gtccaaaccg ggcccctctg ctaaccatgt tcatgccttc     4740

ttctctttcc tacagctcct gggcaacgtg ctggttgttg tgctgtctca tcattttggc     4800

aagaattgaa aaaaggatcc cgtacggcca ccatgttcgt gtttctggtg ctgctgccac     4860

tggtgtccag tcagtgcgtg aacctgacca cacgaacaca gctgccacca gcctacacca     4920

atagcttcac ccgcggagtg tactaccccg acaaggtgtt ccgcagcagc gtgctgcata     4980

gcacccagga tctgtttctg cccttcttca gcaacgtgac ctggttccac gccatccacg     5040

tgtccggcac caatggcacc aagcgcttcg ataatcccgt gctgcccttc aacgatggcg     5100

tgtactttgc cagcaccgag aagtccaata tcatccgcgg ctggatcttc ggcaccacac     5160

tggatagcaa gacccagagc ctgctgatcg tgaacaacgc caccaacgtg gtcatcaaag     5220

tgtgcgagtt ccagttctgc aacgacccct tcctgggcgt ctactaccac aagaacaaca     5280

agagctggat ggaaagcgag ttccgcgtgt acagcagcgc caacaactgc accttcgagt     5340

acgtgtccca gccattcctg atggacctgg aaggcaagca gggcaacttc aagaacctgc     5400

gcgagttcgt gttcaagaac atcgacggct acttcaaaat ctacagcaag cacaccccaa     5460

tcaacctcgt gcgcgatctg ccacagggat tcagtgctct ggaacccctg gtggatctgc     5520

ccatcggcat caacattacc cgctttcaga cactgctggc cctgcaccgc agttacttga     5580

caccaggcga tagcagcagt ggatggacag ctggtgccgc cgcttactac gttggatatc     5640

tgcagccacg cacctttctg ctgaagtaca acgagaacgg caccatcacc gacgccgtgg     5700

attgtgctct cgatcccctg agcgagacaa agtgcaccct gaagtccttc accgtcgaga     5760

agggcatcta ccagaccagc aatttccgcg tgcagcccac cgagagcatc gtgcgcttcc     5820

ccaatatcac caatctgtgc cccttcggcg aggtgttcaa tgccacacgc tttgcctccg     5880

tgtacgcctg gaatcgcaag cgcattagca actgcgtggc cgactactcc gtgctgtaca     5940

atagcgccag cttcagcacc ttcaagtgct acggcgtgtc acccaccaag ctgaacgacc     6000

tgtgcttcac caatgtgtac gccgacagct tcgtgatccg cggagatgaa gtgcgacaga     6060

ttgccccagg ccagaccggc aagatcgccg actacaatta caagctgccc gacgacttca     6120

ccggctgcgt gatcgcctgg aacagcaaca acctggattc caaagtcggc ggcaactaca     6180

actacctgta ccgcctgttc cgcaagagca atctgaagcc cttcgagcgc gacatcagca     6240

ccgaaatcta ccaggccgga agcaccccat gcaacggcgt ggaaggcttc aactgctact     6300

tcccactgca gtcctacgga tttcagccca caaatggcgt gggctaccag ccatatcgag     6360

tggtggtgct gagcttcgaa ctgctgcatg ctccagctac cgtgtgcggc cccaagaaga     6420

gtaccaacct ggtcaagaac aaatgcgtga acttcaactt caacggcctg accggaaccg     6480

gcgtgctgac cgagagtaac aagaagttcc tgccattcca gcagtttggc cgcgacattg     6540

ccgatacaac cgatgccgtt cgcgatcccc agaccttgga gatcctggat attaccccat     6600

gctccttcgg cggcgtgtcc gtgattacac caggcaccaa taccagcaac caggtggccg     6660

ttctgtacca ggatgtgaat tgcacagagg tgcccgtggc cattcacgcc gatcaattga     6720

caccaacatg gcgcgtgtac tccaccggca gcaatgtgtt tcaaacccgc gctggatgcc     6780

tgattggagc cgagcacgtg aacaatagct acgagtgcga tatccccatc ggagccggaa     6840

tctgcgcctc ctatcagacc cagaccaata gtccacgacg agcccgaagt gtggccagcc     6900

agagcatcat tgcctatacc atgagcctgg gcgccgagaa tagcgtggcc tactccaaca     6960

acagcattgc tatccccacc aacttcacca tcagcgtgac caccgagatc ctgccagtgt     7020

ccatgaccaa gaccagcgtg gactgcacca tgtacatctg cggagatagc accgagtgca     7080

gcaacctgct gctgcagtac ggaagtttct gcacccagct gaatcgcgcc ctgacaggca     7140

ttgccgtgga acaggataag aacacccaag aggtgttcgc ccaagtgaag caaatctaca     7200

agaccccacc aatcaaggat ttcggcggct tcaatttcag ccagattctg cccgatccaa     7260

gcaagcccag caagcgcagc ttcatcgagg acctgctgtt caacaaagtg acactggccg     7320

acgccggatt catcaagcag tatggcgatt gcctgggcga tattgccgca cgcgatctga     7380

tttgcgccca gaagtttaac ggactgaccg tcctgccacc actgctgaca gatgagatga     7440

tcgcccagta cacaagtgcc ctgctggccg gaaccattac cagcggatgg acatttggag     7500

ccggtgccgc tctgcagatt cccttcgcta tgcagatggc ctaccgcttc aatggcattg     7560

gcgtgaccca gaatgtgctg tacgagaacc agaagctgat cgccaaccag ttcaacagcg     7620

ccatcggcaa gattcaggac agcctgagta gtaccgccag cgctctggga aagctgcagg     7680

atgtggtcaa ccagaacgct caggccctga acaccctggt taagcagctg agcagcaact     7740

tcggcgccat cagtagcgtg ctgaacgata tcctgagccg cctggataag gtggaagccg     7800

aggtgcagat cgatcgcctg attaccggac gcctgcagtc cctgcagacc tatgtgacac     7860

agcagctgat ccgagccgcc gagattcgag ctagtgctaa tctggccgcc accaagatga     7920

gcgaatgtgt gctgggacag agcaagcgcg tggacttttg cggcaaggga taccacctga     7980

tgagcttccc acagagtgct ccacacggcg tggtgtttct gcatgtgacc tacgtgcccg     8040

ctcaagagaa gaatttcacc accgctccag ccatctgcca cgacggaaag gcccattttc     8100

cacgcgaggg cgtgttcgtt agcaacggca ctcattggtt cgtcacccag cgcaacttct     8160

acgagcccca gatcatcacc accgacaaca ccttcgtcag cggcaactgc gacgtcgtga     8220

tcggcattgt gaacaacacc gtgtacgatc cactgcagcc cgagctggac agcttcaaag     8280

aggaactgga caagtacttt aagaaccaca caagccccga cgtggacctg ggagacatta     8340

gcggaatcaa cgccagcgtg gtcaacatcc agaaagagat tgaccgcctg aacgaggtgg     8400

ccaagaatct gaacgagagc ctgatcgacc tgcaagaact gggcaaatac gagcagtaca     8460

ttaagtggcc ctggtacatc tggctgggct tcattgccgg actgattgcc atcgtgatgg     8520

tcaccattat gctgtgctgc atgaccagtt gctgcagctg cctgaaggga tgctgcagtt     8580

gcggaagctg ctgcaagttc gacgaggatg atagcgagcc agtgctgaag ggcgtcaagc     8640

tgcactacac ctgataacga gcgcgcc                                         8667


<210>  16
<211>  8667
<212>  DNA
<213>  artificial

<220>
<223>  expression plasmid phCMV_Spike_B.1-B

<400>  16
tcgagcggga tcaattcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg       60

tggccaatgc cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg      120

gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca      180

ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa      240

atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc      300

tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg      360

ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt      420

gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca      480

gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat      540

ccctcgacgg atcggccgca attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt      600

tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt      660

gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg      720

ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg      780

cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg      840

cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat      900

aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc      960

gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc     1020

tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga     1080

agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt     1140

ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg     1200

taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc     1260

gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg     1320

gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc     1380

ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg     1440

ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc     1500

gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct     1560

caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt     1620

taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa     1680

aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa     1740

tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc     1800

tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct     1860

gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca     1920

gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt     1980

aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt     2040

gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc     2100

ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcgggttag     2160

ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt     2220

tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac     2280

tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg     2340

cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat     2400

tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc     2460

gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc     2520

tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa     2580

atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg     2640

tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg     2700

cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg     2760

ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct     2820

tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt     2880

ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat     2940

ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca     3000

ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac     3060

gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta     3120

gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg     3180

tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg     3240

ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca     3300

gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta atacgactca     3360

ctatagggcg aattggagct ccaccgcggt ggcggccgct ctagagagct tggcccattg     3420

catacgttgt atccatatca taatatgtac atttatattg gctcatgtcc aacattaccg     3480

ccatgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt     3540

catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga     3600

ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca     3660

atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca     3720

gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg     3780

cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc     3840

tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt     3900

ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt     3960

ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg     4020

acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg     4080

aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg     4140

gaccgatcca gcctccggtc gaccgatcct gagaacttca gggtgagttt ggggaccctt     4200

gattgttctt tctttttcgc tattgtaaaa ttcatgttat atggaggggg caaagttttc     4260

agggtgttgt ttagaatggg aagatgtccc ttgtatcacc atggaccctc atgataattt     4320

tgtttctttc actttctact ctgttgacaa ccattgtctc ctcttatttt cttttcattt     4380

tctgtaactt tttcgttaaa ctttagcttg catttgtaac gaatttttaa attcactttt     4440

gtttatttgt cagattgtaa gtactttctc taatcacttt tttttcaagg caatcagggt     4500

atattatatt gtacttcagc acagttttag agaacaattg ttataattaa atgataaggt     4560

agaatatttc tgcatataaa ttctggctgg cgtggaaata ttcttattgg tagaaacaac     4620

tacaccctgg tcatcatcct gcctttctct ttatggttac aatgatatac actgtttgag     4680

atgaggataa aatactctga gtccaaaccg ggcccctctg ctaaccatgt tcatgccttc     4740

ttctctttcc tacagctcct gggcaacgtg ctggttgttg tgctgtctca tcattttggc     4800

aagaattgaa aaaaggatcc cgtacggcca ccatgttcgt gtttctggtg ctgctgccac     4860

tggtgtccag tcagtgcgtg aacctgacca cacgaacaca gctgccacca gcctacacca     4920

atagcttcac ccgcggagtg tactaccccg acaaggtgtt ccgcagcagc gtgctgcata     4980

gcacccagga tctgtttctg cccttcttca gcaacgtgac ctggttccac gccatccacg     5040

tgtccggcac caatggcacc aagcgcttcg ataatcccgt gctgcccttc aacgatggcg     5100

tgtactttgc cagcaccgag aagtccaata tcatccgcgg ctggatcttc ggcaccacac     5160

tggatagcaa gacccagagc ctgctgatcg tgaacaacgc caccaacgtg gtcatcaaag     5220

tgtgcgagtt ccagttctgc aacgacccct tcctgggcgt ctactaccac aagaacaaca     5280

agagctggat ggaaagcgag ttccgcgtgt acagcagcgc caacaactgc accttcgagt     5340

acgtgtccca gccattcctg atggacctgg aaggcaagca gggcaacttc aagaacctgc     5400

gcgagttcgt gttcaagaac atcgacggct acttcaaaat ctacagcaag cacaccccaa     5460

tcaacctcgt gcgcgatctg ccacagggat tcagtgctct ggaacccctg gtggatctgc     5520

ccatcggcat caacattacc cgctttcaga cactgctggc cctgcaccgc agttacttga     5580

caccaggcga tagcagcagt ggatggacag ctggtgccgc cgcttactac gttggatatc     5640

tgcagccacg cacctttctg ctgaagtaca acgagaacgg caccatcacc gacgccgtgg     5700

attgtgctct cgatcccctg agcgagacaa agtgcaccct gaagtccttc accgtcgaga     5760

agggcatcta ccagaccagc aatttccgcg tgcagcccac cgagagcatc gtgcgcttcc     5820

ccaatatcac caatctgtgc cccttcggcg aggtgttcaa tgccacacgc tttgcctccg     5880

tgtacgcctg gaatcgcaag cgcattagca actgcgtggc cgactactcc gtgctgtaca     5940

atagcgccag cttcagcacc ttcaagtgct acggcgtgtc acccaccaag ctgaacgacc     6000

tgtgcttcac caatgtgtac gccgacagct tcgtgatccg cggagatgaa gtgcgacaga     6060

ttgccccagg ccagaccggc aagatcgccg actacaatta caagctgccc gacgacttca     6120

ccggctgcgt gatcgcctgg aacagcaaca acctggattc caaagtcggc ggcaactaca     6180

actacctgta ccgcctgttc cgcaagagca atctgaagcc cttcgagcgc gacatcagca     6240

ccgaaatcta ccaggccgga agcaccccat gcaacggcgt ggaaggcttc aactgctact     6300

tcccactgca gtcctacgga tttcagccca caaatggcgt gggctaccag ccatatcgag     6360

tggtggtgct gagcttcgaa ctgctgcatg ctccagctac cgtgtgcggc cccaagaaga     6420

gtaccaacct ggtcaagaac aaatgcgtga acttcaactt caacggcctg accggaaccg     6480

gcgtgctgac cgagagtaac aagaagttcc tgccattcca gcagtttggc cgcgacattg     6540

ccgatacaac cgatgccgtt cgcgatcccc agaccttgga gatcctggat attaccccat     6600

gctccttcgg cggcgtgtcc gtgattacac caggcaccaa taccagcaac caggtggccg     6660

ttctgtacca gggcgtgaat tgcacagagg tgcccgtggc cattcacgcc gatcaattga     6720

caccaacatg gcgcgtgtac tccaccggca gcaatgtgtt tcaaacccgc gctggatgcc     6780

tgattggagc cgagcacgtg aacaatagct acgagtgcga tatccccatc ggagccggaa     6840

tctgcgcctc ctatcagacc cagaccaata gtccacgacg agcccgaagt gtggccagcc     6900

agagcatcat tgcctatacc atgagcctgg gcgccgagaa tagcgtggcc tactccaaca     6960

acagcattgc tatccccacc aacttcacca tcagcgtgac caccgagatc ctgccagtgt     7020

ccatgaccaa gaccagcgtg gactgcacca tgtacatctg cggagatagc accgagtgca     7080

gcaacctgct gctgcagtac ggaagtttct gcacccagct gaatcgcgcc ctgacaggca     7140

ttgccgtgga acaggataag aacacccaag aggtgttcgc ccaagtgaag caaatctaca     7200

agaccccacc aatcaaggat ttcggcggct tcaatttcag ccagattctg cccgatccaa     7260

gcaagcccag caagcgcagc ttcatcgagg acctgctgtt caacaaagtg acactggccg     7320

acgccggatt catcaagcag tatggcgatt gcctgggcga tattgccgca cgcgatctga     7380

tttgcgccca gaagtttaac ggactgaccg tcctgccacc actgctgaca gatgagatga     7440

tcgcccagta cacaagtgcc ctgctggccg gaaccattac cagcggatgg acatttggag     7500

ccggtgccgc tctgcagatt cccttcgcta tgcagatggc ctaccgcttc aatggcattg     7560

gcgtgaccca gaatgtgctg tacgagaacc agaagctgat cgccaaccag ttcaacagcg     7620

ccatcggcaa gattcaggac agcctgagta gtaccgccag cgctctggga aagctgcagg     7680

atgtggtcaa ccagaacgct caggccctga acaccctggt taagcagctg agcagcaact     7740

tcggcgccat cagtagcgtg ctgaacgata tcctgagccg cctggataag gtggaagccg     7800

aggtgcagat cgatcgcctg attaccggac gcctgcagtc cctgcagacc tatgtgacac     7860

agcagctgat ccgagccgcc gagattcgag ctagtgctaa tctggccgcc accaagatga     7920

gcgaatgtgt gctgggacag agcaagcgcg tggacttttg cggcaaggga taccacctga     7980

tgagcttccc acagagtgct ccacacggcg tggtgtttct gcatgtgacc tacgtgcccg     8040

ctcaagagaa gaatttcacc accgctccag ccatctgcca cgacggaaag gcccattttc     8100

cacgcgaggg cgtgttcgtt agcaacggca ctcattggtt cgtcacccag cgcaacttct     8160

acgagcccca gatcatcacc accgacaaca ccttcgtcag cggcaactgc gacgtcgtga     8220

tcggcattgt gaacaacacc gtgtacgatc cactgcagcc cgagctggac agcttcaaag     8280

aggaactgga caagtacttt aagaaccaca caagccccga cgtggacctg ggagacatta     8340

gcggaatcaa cgccagcgtg gtcaacatcc agaaagagat tgaccgcctg aacgaggtgg     8400

ccaagaatct gaacgagagc ctgatcgacc tgcaagaact gggcaaatac gagcagtaca     8460

ttaagtggcc ctggtacatc tggctgggct tcattgccgg actgattgcc atcgtgatgg     8520

tcaccattat gctgtgctgc atgaccagtt gctgcagctg cctgaaggga tgctgcagtt     8580

gcggaagctg ctgcaagttc gacgaggatg atagcgagcc agtgctgaag ggcgtcaagc     8640

tgcactacac ctgataacga gcgcgcc                                         8667


<210>  17
<211>  8696
<212>  DNA
<213>  artificial

<220>
<223>  expression plasmid phCMV_Spike_B.1.1.7

<400>  17
gatccacaag tttgtacaaa aaagcaggct gccaccatgt tcgtgtttct ggtgctgctg       60

ccactggtgt ccagtcagtg cgtgaacctg accacacgaa cacagctgcc accagcctac      120

accaatagct tcacccgcgg agtgtactac cccgacaagg tgttccgcag cagcgtgctg      180

catagcaccc aggatctgtt tctgcccttc ttcagcaacg tgacctggtt ccacgccatc      240

tccggcacca atggcaccaa gcgcttcgat aatcccgtgc tgcccttcaa cgatggcgtg      300

tactttgcca gcaccgagaa gtccaatatc atccgcggct ggatcttcgg caccacactg      360

gatagcaaga cccagagcct gctgatcgtg aacaacgcca ccaacgtggt catcaaagtg      420

tgcgagttcc agttctgcaa cgaccccttc ctgggcgtct accacaagaa caacaagagc      480

tggatggaaa gcgagttccg cgtgtacagc agcgccaaca actgcacctt cgagtacgtg      540

tcccagccat tcctgatgga cctggaaggc aagcagggca acttcaagaa cctgcgcgag      600

ttcgtgttca agaacatcga cggctacttc aaaatctaca gcaagcacac cccaatcaac      660

ctcgtgcgcg atctgccaca gggattcagt gctctggaac ccctggtgga tctgcccatc      720

ggcatcaaca ttacccgctt tcagacactg ctggccctgc accgcagtta cttgacacca      780

ggcgatagca gcagtggatg gacagctggt gccgccgctt actacgttgg atatctgcag      840

ccacgcacct ttctgctgaa gtacaacgag aacggcacca tcaccgacgc cgtggattgt      900

gctctcgatc ccctgagcga gacaaagtgc accctgaagt ccttcaccgt cgagaagggc      960

atctaccaga ccagcaattt ccgcgtgcag cccaccgaga gcatcgtgcg cttccccaat     1020

atcaccaatc tgtgcccctt cggcgaggtg ttcaatgcca cacgctttgc ctccgtgtac     1080

gcctggaatc gcaagcgcat tagcaactgc gtggccgact actccgtgct gtacaatagc     1140

gccagcttca gcaccttcaa gtgctacggc gtgtcaccca ccaagctgaa cgacctgtgc     1200

ttcaccaatg tgtacgccga cagcttcgtg atccgcggag atgaagtgcg acagattgcc     1260

ccaggccaga ccggcaagat cgccgactac aattacaagc tgcccgacga cttcaccggc     1320

tgcgtgatcg cctggaacag caacaacctg gattccaaag tcggcggcaa ctacaactac     1380

ctgtaccgcc tgttccgcaa gagcaatctg aagcccttcg agcgcgacat cagcaccgaa     1440

atctaccagg ccggaagcac cccatgcaac ggcgtggaag gcttcaactg ctacttccca     1500

ctgcagtcct acggatttca gcccacatat ggcgtgggct accagccata tcgagtggtg     1560

gtgctgagct tcgaactgct gcatgctcca gctaccgtgt gcggccccaa gaagagtacc     1620

aacctggtca agaacaaatg cgtgaacttc aacttcaacg gcctgaccgg aaccggcgtg     1680

ctgaccgaga gtaacaagaa gttcctgcca ttccagcagt ttggccgcga cattgacgat     1740

acaaccgatg ccgttcgcga tccccagacc ttggagatcc tggatattac cccatgctcc     1800

ttcggcggcg tgtccgtgat tacaccaggc accaatacca gcaaccaggt ggccgttctg     1860

taccagggcg tgaattgcac agaggtgccc gtggccattc acgccgatca attgacacca     1920

acatggcgcg tgtactccac cggcagcaat gtgtttcaaa cccgcgctgg atgcctgatt     1980

ggagccgagc acgtgaacaa tagctacgag tgcgatatcc ccatcggagc cggaatctgc     2040

gcctcctatc agacccagac caatagtcac cgacgagccc gaagtgtggc cagccagagc     2100

atcattgcct ataccatgag cctgggcgcc gagaatagcg tggcctactc caacaacagc     2160

attgctatcc ccatcaactt caccatcagc gtgaccaccg agatcctgcc agtgtccatg     2220

accaagacca gcgtggactg caccatgtac atctgcggag atagcaccga gtgcagcaac     2280

ctgctgctgc agtacggaag tttctgcacc cagctgaatc gcgccctgac aggcattgcc     2340

gtggaacagg ataagaacac ccaagaggtg ttcgcccaag tgaagcaaat ctacaagacc     2400

ccaccaatca aggatttcgg cggcttcaat ttcagccaga ttctgcccga tccaagcaag     2460

cccagcaagc gcagcttcat cgaggacctg ctgttcaaca aagtgacact ggccgacgcc     2520

ggattcatca agcagtatgg cgattgcctg ggcgatattg ccgcacgcga tctgatttgc     2580

gcccagaagt ttaacggact gaccgtcctg ccaccactgc tgacagatga gatgatcgcc     2640

cagtacacaa gtgccctgct ggccggaacc attaccagcg gatggacatt tggagccggt     2700

gccgctctgc agattccctt cgctatgcag atggcctacc gcttcaatgg cattggcgtg     2760

acccagaatg tgctgtacga gaaccagaag ctgatcgcca accagttcaa cagcgccatc     2820

ggcaagattc aggacagcct gagtagtacc gccagcgctc tgggaaagct gcaggatgtg     2880

gtcaaccaga acgctcaggc cctgaacacc ctggttaagc agctgagcag caacttcggc     2940

gccatcagta gcgtgctgaa cgatatcctg gcccgcctgg ataaggtgga agccgaggtg     3000

cagatcgatc gcctgattac cggacgcctg cagtccctgc agacctatgt gacacagcag     3060

ctgatccgag ccgccgagat tcgagctagt gctaatctgg ccgccaccaa gatgagcgaa     3120

tgtgtgctgg gacagagcaa gcgcgtggac ttttgcggca agggatacca cctgatgagc     3180

ttcccacaga gtgctccaca cggcgtggtg tttctgcatg tgacctacgt gcccgctcaa     3240

gagaagaatt tcaccaccgc tccagccatc tgccacgacg gaaaggccca ttttccacgc     3300

gagggcgtgt tcgttagcaa cggcactcat tggttcgtca cccagcgcaa cttctacgag     3360

ccccagatca tcaccaccca caacaccttc gtcagcggca actgcgacgt cgtgatcggc     3420

attgtgaaca acaccgtgta cgatccactg cagcccgagc tggacagctt caaagaggaa     3480

ctggacaagt actttaagaa ccacacaagc cccgacgtgg acctgggaga cattagcgga     3540

atcaacgcca gcgtggtcaa catccagaaa gagattgacc gcctgaacga ggtggccaag     3600

aatctgaacg agagcctgat cgacctgcaa gaactgggca aatacgagca gtacattaag     3660

tggccctggt acatctggct gggcttcatt gccggactga ttgccatcgt gatggtcacc     3720

attatgctgt gctgcatgac cagttgctgc agctgcctga agggatgctg cagttgcgga     3780

agctgctgca agttcgacga ggatgatagc gagccagtgc tgaagggcgt caagctgcac     3840

tacacctgat agtaaaccca gctttcttgt acaaagtggt ctcgagcggg atcaattcac     3900

tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca     3960

caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct     4020

tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa     4080

ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa     4140

tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg     4200

ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca     4260

tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc     4320

tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg     4380

actactccca gtcatagctg tccctcttct cttatggaga tccctcgacg gatcggccgc     4440

aattcgtaat catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac     4500

acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac     4560

tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc     4620

tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg     4680

cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc     4740

actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt     4800

gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc     4860

ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa     4920

acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc     4980

ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg     5040

cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc     5100

tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc     5160

gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca     5220

ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact     5280

acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg     5340

gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt     5400

ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct     5460

tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga     5520

gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa     5580

tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac     5640

ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga     5700

taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc     5760

cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca     5820

gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta     5880

gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg     5940

tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc     6000

gagttacatg atcccccatg ttgtgcaaaa aagcgggtta gctccttcgg tcctccgatc     6060

gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat     6120

tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag     6180

tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat     6240

aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg     6300

cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca     6360

cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga     6420

aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc     6480

ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata     6540

tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg     6600

ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca     6660

gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga     6720

ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg     6780

actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat     6840

caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag     6900

ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga     6960

agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa     7020

ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc     7080

tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga     7140

aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac     7200

gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattggagc     7260

tccaccgcgg tggcggccgc tctagagagc ttggcccatt gcatacgttg tatccatatc     7320

ataatatgta catttatatt ggctcatgtc caacattacc gccatgttga cattgattat     7380

tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt     7440

tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc     7500

cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac     7560

gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata     7620

tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc     7680

agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta     7740

ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac     7800

ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc     7860

aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc     7920

gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga     7980

gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccggt     8040

cgaccgatcc tgagaacttc agggtgagtt tggggaccct tgattgttct ttctttttcg     8100

ctattgtaaa attcatgtta tatggagggg gcaaagtttt cagggtgttg tttagaatgg     8160

gaagatgtcc cttgtatcac catggaccct catgataatt ttgtttcttt cactttctac     8220

tctgttgaca accattgtct cctcttattt tcttttcatt ttctgtaact ttttcgttaa     8280

actttagctt gcatttgtaa cgaattttta aattcacttt tgtttatttg tcagattgta     8340

agtactttct ctaatcactt ttttttcaag gcaatcaggg tatattatat tgtacttcag     8400

cacagtttta gagaacaatt gttataatta aatgataagg tagaatattt ctgcatataa     8460

attctggctg gcgtggaaat attcttattg gtagaaacaa ctacaccctg gtcatcatcc     8520

tgcctttctc tttatggtta caatgatata cactgtttga gatgaggata aaatactctg     8580

agtccaaacc gggcccctct gctaaccatg ttcatgcctt cttctctttc ctacagctcc     8640

tgggcaacgt gctggttgtt gtgctgtctc atcattttgg caagaattga aaaaag         8696


<210>  18
<211>  8696
<212>  DNA
<213>  artificial

<220>
<223>  expression plasmid phCMV_Spike_B.1.351

<400>  18
gatccacaag tttgtacaaa aaagcaggct gccaccatgt tcgtgtttct ggtgctgctg       60

ccactggtgt ccagtcagtg cgtgaacttc accacacgaa cacagctgcc accagcctac      120

accaatagct tcacccgcgg agtgtactac cccgacaagg tgttccgcag cagcgtgctg      180

catagcaccc aggatctgtt tctgcccttc ttcagcaacg tgacctggtt ccacgccatc      240

cacgtgtccg gcaccaatgg caccaagcgc ttcgctaatc ccgtgctgcc cttcaacgat      300

ggcgtgtact ttgccagcac cgagaagtcc aatatcatcc gcggctggat cttcggcacc      360

acactggata gcaagaccca gagcctgctg atcgtgaaca acgccaccaa cgtggtcatc      420

aaagtgtgcg agttccagtt ctgcaacgac cccttcctgg gcgtctacta ccacaagaac      480

aacaagagct ggatggaaag cgagttccgc gtgtacagca gcgccaacaa ctgcaccttc      540

gagtacgtgt cccagccatt cctgatggac ctggaaggca agcagggcaa cttcaagaac      600

ctgcgcgagt tcgtgttcaa gaacatcgac ggctacttca aaatctacag caagcacacc      660

ccaatcaacc tcgtgcgcgg cctgccacag ggattcagtg ctctggaacc cctggtggat      720

ctgcccatcg gcatcaacat tacccgcttt cagacactgc accgcagtta cttgacacca      780

ggcgatagca gcagtggatg gacagctggt gccgccgctt actacgttgg atatctgcag      840

ccacgcacct ttctgctgaa gtacaacgag aacggcacca tcaccgacgc cgtggattgt      900

gctctcgatc ccctgagcga gacaaagtgc accctgaagt ccttcaccgt cgagaagggc      960

atctaccaga ccagcaattt ccgcgtgcag cccaccgaga gcatcgtgcg cttccccaat     1020

atcaccaatc tgtgcccctt cggcgaggtg ttcaatgcca cacgctttgc ctccgtgtac     1080

gcctggaatc gcaagcgcat tagcaactgc gtggccgact actccgtgct gtacaatagc     1140

gccagcttca gcaccttcaa gtgctacggc gtgtcaccca ccaagctgaa cgacctgtgc     1200

ttcaccaatg tgtacgccga cagcttcgtg atccgcggag atgaagtgcg acagattgcc     1260

ccaggccaga ccggcaacat cgccgactac aattacaagc tgcccgacga cttcaccggc     1320

tgcgtgatcg cctggaacag caacaacctg gattccaaag tcggcggcaa ctacaactac     1380

ctgtaccgcc tgttccgcaa gagcaatctg aagcccttcg agcgcgacat cagcaccgaa     1440

atctaccagg ccggaagcac cccatgcaac ggcgtgaaag gcttcaactg ctacttccca     1500

ctgcagtcct acggatttca gcccacatat ggcgtgggct accagccata tcgagtggtg     1560

gtgctgagct tcgaactgct gcatgctcca gctaccgtgt gcggccccaa gaagagtacc     1620

aacctggtca agaacaaatg cgtgaacttc aacttcaacg gcctgaccgg aaccggcgtg     1680

ctgaccgaga gtaacaagaa gttcctgcca ttccagcagt ttggccgcga cattgccgat     1740

acaaccgatg ccgttcgcga tccccagacc ttggagatcc tggatattac cccatgctcc     1800

ttcggcggcg tgtccgtgat tacaccaggc accaatacca gcaaccaggt ggccgttctg     1860

taccagggcg tgaattgcac agaggtgccc gtggccattc acgccgatca attgacacca     1920

acatggcgcg tgtactccac cggcagcaat gtgtttcaaa cccgcgctgg atgcctgatt     1980

ggagccgagc acgtgaacaa tagctacgag tgcgatatcc ccatcggagc cggaatctgc     2040

gcctcctatc agacccagac caatagtcca cgacgagccc gaagtgtggc cagccagagc     2100

atcattgcct ataccatgag cctgggcgtg gagaatagcg tggcctactc caacaacagc     2160

attgctatcc ccaccaactt caccatcagc gtgaccaccg agatcctgcc agtgtccatg     2220

accaagacca gcgtggactg caccatgtac atctgcggag atagcaccga gtgcagcaac     2280

ctgctgctgc agtacggaag tttctgcacc cagctgaatc gcgccctgac aggcattgcc     2340

gtggaacagg ataagaacac ccaagaggtg ttcgcccaag tgaagcaaat ctacaagacc     2400

ccaccaatca aggatttcgg cggcttcaat ttcagccaga ttctgcccga tccaagcaag     2460

cccagcaagc gcagcttcat cgaggacctg ctgttcaaca aagtgacact ggccgacgcc     2520

ggattcatca agcagtatgg cgattgcctg ggcgatattg ccgcacgcga tctgatttgc     2580

gcccagaagt ttaacggact gaccgtcctg ccaccactgc tgacagatga gatgatcgcc     2640

cagtacacaa gtgccctgct ggccggaacc attaccagcg gatggacatt tggagccggt     2700

gccgctctgc agattccctt cgctatgcag atggcctacc gcttcaatgg cattggcgtg     2760

acccagaatg tgctgtacga gaaccagaag ctgatcgcca accagttcaa cagcgccatc     2820

ggcaagattc aggacagcct gagtagtacc gccagcgctc tgggaaagct gcaggatgtg     2880

gtcaaccaga acgctcaggc cctgaacacc ctggttaagc agctgagcag caacttcggc     2940

gccatcagta gcgtgctgaa cgatatcctg agccgcctgg ataaggtgga agccgaggtg     3000

cagatcgatc gcctgattac cggacgcctg cagtccctgc agacctatgt gacacagcag     3060

ctgatccgag ccgccgagat tcgagctagt gctaatctgg ccgccaccaa gatgagcgaa     3120

tgtgtgctgg gacagagcaa gcgcgtggac ttttgcggca agggatacca cctgatgagc     3180

ttcccacaga gtgctccaca cggcgtggtg tttctgcatg tgacctacgt gcccgctcaa     3240

gagaagaatt tcaccaccgc tccagccatc tgccacgacg gaaaggccca ttttccacgc     3300

gagggcgtgt tcgttagcaa cggcactcat tggttcgtca cccagcgcaa cttctacgag     3360

ccccagatca tcaccaccga caacaccttc gtcagcggca actgcgacgt cgtgatcggc     3420

attgtgaaca acaccgtgta cgatccactg cagcccgagc tggacagctt caaagaggaa     3480

ctggacaagt actttaagaa ccacacaagc cccgacgtgg acctgggaga cattagcgga     3540

atcaacgcca gcgtggtcaa catccagaaa gagattgacc gcctgaacga ggtggccaag     3600

aatctgaacg agagcctgat cgacctgcaa gaactgggca aatacgagca gtacattaag     3660

tggccctggt acatctggct gggcttcatt gccggactga ttgccatcgt gatggtcacc     3720

attatgctgt gctgcatgac cagttgctgc agctgcctga agggatgctg cagttgcgga     3780

agctgctgca agttcgacga ggatgatagc gagccagtgc tgaagggcgt caagctgcac     3840

tacacctgat agtaaaccca gctttcttgt acaaagtggt ctcgagcggg atcaattcac     3900

tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca     3960

caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct     4020

tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa     4080

ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa     4140

tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg     4200

ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca     4260

tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc     4320

tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg     4380

actactccca gtcatagctg tccctcttct cttatggaga tccctcgacg gatcggccgc     4440

aattcgtaat catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac     4500

acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac     4560

tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc     4620

tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg     4680

cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc     4740

actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt     4800

gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc     4860

ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa     4920

acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc     4980

ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg     5040

cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc     5100

tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc     5160

gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca     5220

ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact     5280

acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg     5340

gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt     5400

ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct     5460

tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga     5520

gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa     5580

tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac     5640

ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga     5700

taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc     5760

cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca     5820

gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta     5880

gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg     5940

tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc     6000

gagttacatg atcccccatg ttgtgcaaaa aagcgggtta gctccttcgg tcctccgatc     6060

gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat     6120

tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag     6180

tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat     6240

aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg     6300

cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca     6360

cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga     6420

aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc     6480

ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata     6540

tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg     6600

ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca     6660

gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga     6720

ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg     6780

actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat     6840

caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag     6900

ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga     6960

agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa     7020

ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc     7080

tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga     7140

aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac     7200

gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattggagc     7260

tccaccgcgg tggcggccgc tctagagagc ttggcccatt gcatacgttg tatccatatc     7320

ataatatgta catttatatt ggctcatgtc caacattacc gccatgttga cattgattat     7380

tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt     7440

tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc     7500

cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac     7560

gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata     7620

tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc     7680

agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta     7740

ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac     7800

ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc     7860

aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc     7920

gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga     7980

gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccggt     8040

cgaccgatcc tgagaacttc agggtgagtt tggggaccct tgattgttct ttctttttcg     8100

ctattgtaaa attcatgtta tatggagggg gcaaagtttt cagggtgttg tttagaatgg     8160

gaagatgtcc cttgtatcac catggaccct catgataatt ttgtttcttt cactttctac     8220

tctgttgaca accattgtct cctcttattt tcttttcatt ttctgtaact ttttcgttaa     8280

actttagctt gcatttgtaa cgaattttta aattcacttt tgtttatttg tcagattgta     8340

agtactttct ctaatcactt ttttttcaag gcaatcaggg tatattatat tgtacttcag     8400

cacagtttta gagaacaatt gttataatta aatgataagg tagaatattt ctgcatataa     8460

attctggctg gcgtggaaat attcttattg gtagaaacaa ctacaccctg gtcatcatcc     8520

tgcctttctc tttatggtta caatgatata cactgtttga gatgaggata aaatactctg     8580

agtccaaacc gggcccctct gctaaccatg ttcatgcctt cttctctttc ctacagctcc     8640

tgggcaacgt gctggttgtt gtgctgtctc atcattttgg caagaattga aaaaag         8696


<210>  19
<211>  8667
<212>  DNA
<213>  artificial

<220>
<223>  expression plasmid phCMV_Spike_P.1

<400>  19
tcgagcggga tcaattcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg       60

tggccaatgc cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg      120

gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca      180

ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa      240

atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc      300

tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg      360

ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt      420

gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca      480

gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat      540

ccctcgacgg atcggccgca attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt      600

tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt      660

gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg      720

ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg      780

cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg      840

cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat      900

aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc      960

gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc     1020

tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga     1080

agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt     1140

ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg     1200

taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc     1260

gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg     1320

gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc     1380

ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg     1440

ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc     1500

gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct     1560

caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt     1620

taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa     1680

aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa     1740

tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc     1800

tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct     1860

gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca     1920

gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt     1980

aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt     2040

gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc     2100

ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcgggttag     2160

ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt     2220

tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac     2280

tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg     2340

cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat     2400

tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc     2460

gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc     2520

tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa     2580

atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg     2640

tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg     2700

cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg     2760

ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct     2820

tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt     2880

ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat     2940

ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca     3000

ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac     3060

gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta     3120

gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg     3180

tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg     3240

ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca     3300

gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta atacgactca     3360

ctatagggcg aattggagct ccaccgcggt ggcggccgct ctagagagct tggcccattg     3420

catacgttgt atccatatca taatatgtac atttatattg gctcatgtcc aacattaccg     3480

ccatgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt     3540

catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga     3600

ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca     3660

atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca     3720

gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg     3780

cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc     3840

tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt     3900

ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt     3960

ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg     4020

acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg     4080

aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg     4140

gaccgatcca gcctccggtc gaccgatcct gagaacttca gggtgagttt ggggaccctt     4200

gattgttctt tctttttcgc tattgtaaaa ttcatgttat atggaggggg caaagttttc     4260

agggtgttgt ttagaatggg aagatgtccc ttgtatcacc atggaccctc atgataattt     4320

tgtttctttc actttctact ctgttgacaa ccattgtctc ctcttatttt cttttcattt     4380

tctgtaactt tttcgttaaa ctttagcttg catttgtaac gaatttttaa attcactttt     4440

gtttatttgt cagattgtaa gtactttctc taatcacttt tttttcaagg caatcagggt     4500

atattatatt gtacttcagc acagttttag agaacaattg ttataattaa atgataaggt     4560

agaatatttc tgcatataaa ttctggctgg cgtggaaata ttcttattgg tagaaacaac     4620

tacaccctgg tcatcatcct gcctttctct ttatggttac aatgatatac actgtttgag     4680

atgaggataa aatactctga gtccaaaccg ggcccctctg ctaaccatgt tcatgccttc     4740

ttctctttcc tacagctcct gggcaacgtg ctggttgttg tgctgtctca tcattttggc     4800

aagaattgaa aaaaggatcc cgtacggcca ccatgttcgt gtttctggtg ctgctgccac     4860

tggtgtccag tcagtgcgtg aacttcacca accgaacaca gctgccatcc gcctacacca     4920

atagcttcac ccgcggagtg tactaccccg acaaggtgtt ccgcagcagc gtgctgcata     4980

gcacccagga tctgtttctg cccttcttca gcaacgtgac ctggttccac gccatccacg     5040

tgtccggcac caatggcacc aagcgcttcg ataatcccgt gctgcccttc aacgatggcg     5100

tgtactttgc cagcaccgag aagtccaata tcatccgcgg ctggatcttc ggcaccacac     5160

tggatagcaa gacccagagc ctgctgatcg tgaacaacgc caccaacgtg gtcatcaaag     5220

tgtgcgagtt ccagttctgc aactacccct tcctgggcgt ctactaccac aagaacaaca     5280

agagctggat ggaaagcgag ttccgcgtgt acagcagcgc caacaactgc accttcgagt     5340

acgtgtccca gccattcctg atggacctgg aaggcaagca gggcaacttc aagaacctgt     5400

ccgagttcgt gttcaagaac atcgacggct acttcaaaat ctacagcaag cacaccccaa     5460

tcaacctcgt gcgcgatctg ccacagggat tcagtgctct ggaacccctg gtggatctgc     5520

ccatcggcat caacattacc cgctttcaga cactgctggc cctgcaccgc agttacttga     5580

caccaggcga tagcagcagt ggatggacag ctggtgccgc cgcttactac gttggatatc     5640

tgcagccacg cacctttctg ctgaagtaca acgagaacgg caccatcacc gacgccgtgg     5700

attgtgctct cgatcccctg agcgagacaa agtgcaccct gaagtccttc accgtcgaga     5760

agggcatcta ccagaccagc aatttccgcg tgcagcccac cgagagcatc gtgcgcttcc     5820

ccaatatcac caatctgtgc cccttcggcg aggtgttcaa tgccacacgc tttgcctccg     5880

tgtacgcctg gaatcgcaag cgcattagca actgcgtggc cgactactcc gtgctgtaca     5940

atagcgccag cttcagcacc ttcaagtgct acggcgtgtc acccaccaag ctgaacgacc     6000

tgtgcttcac caatgtgtac gccgacagct tcgtgatccg cggagatgaa gtgcgacaga     6060

ttgccccagg ccagaccggc accatcgccg actacaatta caagctgccc gacgacttca     6120

ccggctgcgt gatcgcctgg aacagcaaca acctggattc caaagtcggc ggcaactaca     6180

actacctgta ccgcctgttc cgcaagagca atctgaagcc cttcgagcgc gacatcagca     6240

ccgaaatcta ccaggccgga agcaccccat gcaacggcgt gaaaggcttc aactgctact     6300

tcccactgca gtcctacgga tttcagccca catatggcgt gggctaccag ccatatcgag     6360

tggtggtgct gagcttcgaa ctgctgcatg ctccagctac cgtgtgcggc cccaagaaga     6420

gtaccaacct ggtcaagaac aaatgcgtga acttcaactt caacggcctg accggaaccg     6480

gcgtgctgac cgagagtaac aagaagttcc tgccattcca gcagtttggc cgcgacattg     6540

ccgatacaac cgatgccgtt cgcgatcccc agaccttgga gatcctggat attaccccat     6600

gctccttcgg cggcgtgtcc gtgattacac caggcaccaa taccagcaac caggtggccg     6660

ttctgtacca gggcgtgaat tgcacagagg tgcccgtggc cattcacgcc gatcaattga     6720

caccaacatg gcgcgtgtac tccaccggca gcaatgtgtt tcaaacccgc gctggatgcc     6780

tgattggagc cgagtacgtg aacaatagct acgagtgcga tatccccatc ggagccggaa     6840

tctgcgcctc ctatcagacc cagaccaata gtccacgacg agcccgaagt gtggccagcc     6900

agagcatcat tgcctatacc atgagcctgg gcgccgagaa tagcgtggcc tactccaaca     6960

acagcattgc tatccccacc aacttcacca tcagcgtgac caccgagatc ctgccagtgt     7020

ccatgaccaa gaccagcgtg gactgcacca tgtacatctg cggagatagc accgagtgca     7080

gcaacctgct gctgcagtac ggaagtttct gcacccagct gaatcgcgcc ctgacaggca     7140

ttgccgtgga acaggataag aacacccaag aggtgttcgc ccaagtgaag caaatctaca     7200

agaccccacc aatcaaggat ttcggcggct tcaatttcag ccagattctg cccgatccaa     7260

gcaagcccag caagcgcagc ttcatcgagg acctgctgtt caacaaagtg acactggccg     7320

acgccggatt catcaagcag tatggcgatt gcctgggcga tattgccgca cgcgatctga     7380

tttgcgccca gaagtttaac ggactgaccg tcctgccacc actgctgaca gatgagatga     7440

tcgcccagta cacaagtgcc ctgctggccg gaaccattac cagcggatgg acatttggag     7500

ccggtgccgc tctgcagatt cccttcgcta tgcagatggc ctaccgcttc aatggcattg     7560

gcgtgaccca gaatgtgctg tacgagaacc agaagctgat cgccaaccag ttcaacagcg     7620

ccatcggcaa gattcaggac agcctgagta gtaccgccag cgctctggga aagctgcagg     7680

atgtggtcaa ccagaacgct caggccctga acaccctggt taagcagctg agcagcaact     7740

tcggcgccat cagtagcgtg ctgaacgata tcctgagccg cctggataag gtggaagccg     7800

aggtgcagat cgatcgcctg attaccggac gcctgcagtc cctgcagacc tatgtgacac     7860

agcagctgat ccgagccgcc gagattcgag ctagtgctaa tctggccgcc atcaagatga     7920

gcgaatgtgt gctgggacag agcaagcgcg tggacttttg cggcaaggga taccacctga     7980

tgagcttccc acagagtgct ccacacggcg tggtgtttct gcatgtgacc tacgtgcccg     8040

ctcaagagaa gaatttcacc accgctccag ccatctgcca cgacggaaag gcccattttc     8100

cacgcgaggg cgtgttcgtt agcaacggca ctcattggtt cgtcacccag cgcaacttct     8160

acgagcccca gatcatcacc accgacaaca ccttcgtcag cggcaactgc gacgtcgtga     8220

tcggcattgt gaacaacacc gtgtacgatc cactgcagcc cgagctggac agcttcaaag     8280

aggaactgga caagtacttt aagaaccaca caagccccga cgtggacctg ggagacatta     8340

gcggaatcaa cgccagcgtg gtcaacatcc agaaagagat tgaccgcctg aacgaggtgg     8400

ccaagaatct gaacgagagc ctgatcgacc tgcaagaact gggcaaatac gagcagtaca     8460

ttaagtggcc ctggtacatc tggctgggct tcattgccgg actgattgcc atcgtgatgg     8520

tcaccattat gctgtgctgc atgaccagtt gctgcagctg cctgaaggga tgctgcagtt     8580

gcggaagctg ctgcaagttc gacgaggatg atagcgagcc agtgctgaag ggcgtcaagc     8640

tgcactacac ctgataacga gcgcgcc                                         8667


<210>  20
<211>  3822
<212>  DNA
<213>  artificial

<220>
<223>  codon optimized nucleotide sequence encoding Spike Protein B.1-G

<400>  20
atgttcgtgt ttctggtgct gctgccactg gtgtccagtc agtgcgtgaa cctgaccaca       60

cgaacacagc tgccaccagc ctacaccaat agcttcaccc gcggagtgta ctaccccgac      120

aaggtgttcc gcagcagcgt gctgcatagc acccaggatc tgtttctgcc cttcttcagc      180

aacgtgacct ggttccacgc catccacgtg tccggcacca atggcaccaa gcgcttcgat      240

aatcccgtgc tgcccttcaa cgatggcgtg tactttgcca gcaccgagaa gtccaatatc      300

atccgcggct ggatcttcgg caccacactg gatagcaaga cccagagcct gctgatcgtg      360

aacaacgcca ccaacgtggt catcaaagtg tgcgagttcc agttctgcaa cgaccccttc      420

ctgggcgtct actaccacaa gaacaacaag agctggatgg aaagcgagtt ccgcgtgtac      480

agcagcgcca acaactgcac cttcgagtac gtgtcccagc cattcctgat ggacctggaa      540

ggcaagcagg gcaacttcaa gaacctgcgc gagttcgtgt tcaagaacat cgacggctac      600

ttcaaaatct acagcaagca caccccaatc aacctcgtgc gcgatctgcc acagggattc      660

agtgctctgg aacccctggt ggatctgccc atcggcatca acattacccg ctttcagaca      720

ctgctggccc tgcaccgcag ttacttgaca ccaggcgata gcagcagtgg atggacagct      780

ggtgccgccg cttactacgt tggatatctg cagccacgca cctttctgct gaagtacaac      840

gagaacggca ccatcaccga cgccgtggat tgtgctctcg atcccctgag cgagacaaag      900

tgcaccctga agtccttcac cgtcgagaag ggcatctacc agaccagcaa tttccgcgtg      960

cagcccaccg agagcatcgt gcgcttcccc aatatcacca atctgtgccc cttcggcgag     1020

gtgttcaatg ccacacgctt tgcctccgtg tacgcctgga atcgcaagcg cattagcaac     1080

tgcgtggccg actactccgt gctgtacaat agcgccagct tcagcacctt caagtgctac     1140

ggcgtgtcac ccaccaagct gaacgacctg tgcttcacca atgtgtacgc cgacagcttc     1200

gtgatccgcg gagatgaagt gcgacagatt gccccaggcc agaccggcaa gatcgccgac     1260

tacaattaca agctgcccga cgacttcacc ggctgcgtga tcgcctggaa cagcaacaac     1320

ctggattcca aagtcggcgg caactacaac tacctgtacc gcctgttccg caagagcaat     1380

ctgaagccct tcgagcgcga catcagcacc gaaatctacc aggccggaag caccccatgc     1440

aacggcgtgg aaggcttcaa ctgctacttc ccactgcagt cctacggatt tcagcccaca     1500

aatggcgtgg gctaccagcc atatcgagtg gtggtgctga gcttcgaact gctgcatgct     1560

ccagctaccg tgtgcggccc caagaagagt accaacctgg tcaagaacaa atgcgtgaac     1620

ttcaacttca acggcctgac cggaaccggc gtgctgaccg agagtaacaa gaagttcctg     1680

ccattccagc agtttggccg cgacattgcc gatacaaccg atgccgttcg cgatccccag     1740

accttggaga tcctggatat taccccatgc tccttcggcg gcgtgtccgt gattacacca     1800

ggcaccaata ccagcaacca ggtggccgtt ctgtaccagg atgtgaattg cacagaggtg     1860

cccgtggcca ttcacgccga tcaattgaca ccaacatggc gcgtgtactc caccggcagc     1920

aatgtgtttc aaacccgcgc tggatgcctg attggagccg agcacgtgaa caatagctac     1980

gagtgcgata tccccatcgg agccggaatc tgcgcctcct atcagaccca gaccaatagt     2040

ccacgacgag cccgaagtgt ggccagccag agcatcattg cctataccat gagcctgggc     2100

gccgagaata gcgtggccta ctccaacaac agcattgcta tccccaccaa cttcaccatc     2160

agcgtgacca ccgagatcct gccagtgtcc atgaccaaga ccagcgtgga ctgcaccatg     2220

tacatctgcg gagatagcac cgagtgcagc aacctgctgc tgcagtacgg aagtttctgc     2280

acccagctga atcgcgccct gacaggcatt gccgtggaac aggataagaa cacccaagag     2340

gtgttcgccc aagtgaagca aatctacaag accccaccaa tcaaggattt cggcggcttc     2400

aatttcagcc agattctgcc cgatccaagc aagcccagca agcgcagctt catcgaggac     2460

ctgctgttca acaaagtgac actggccgac gccggattca tcaagcagta tggcgattgc     2520

ctgggcgata ttgccgcacg cgatctgatt tgcgcccaga agtttaacgg actgaccgtc     2580

ctgccaccac tgctgacaga tgagatgatc gcccagtaca caagtgccct gctggccgga     2640

accattacca gcggatggac atttggagcc ggtgccgctc tgcagattcc cttcgctatg     2700

cagatggcct accgcttcaa tggcattggc gtgacccaga atgtgctgta cgagaaccag     2760

aagctgatcg ccaaccagtt caacagcgcc atcggcaaga ttcaggacag cctgagtagt     2820

accgccagcg ctctgggaaa gctgcaggat gtggtcaacc agaacgctca ggccctgaac     2880

accctggtta agcagctgag cagcaacttc ggcgccatca gtagcgtgct gaacgatatc     2940

ctgagccgcc tggataaggt ggaagccgag gtgcagatcg atcgcctgat taccggacgc     3000

ctgcagtccc tgcagaccta tgtgacacag cagctgatcc gagccgccga gattcgagct     3060

agtgctaatc tggccgccac caagatgagc gaatgtgtgc tgggacagag caagcgcgtg     3120

gacttttgcg gcaagggata ccacctgatg agcttcccac agagtgctcc acacggcgtg     3180

gtgtttctgc atgtgaccta cgtgcccgct caagagaaga atttcaccac cgctccagcc     3240

atctgccacg acggaaaggc ccattttcca cgcgagggcg tgttcgttag caacggcact     3300

cattggttcg tcacccagcg caacttctac gagccccaga tcatcaccac cgacaacacc     3360

ttcgtcagcg gcaactgcga cgtcgtgatc ggcattgtga acaacaccgt gtacgatcca     3420

ctgcagcccg agctggacag cttcaaagag gaactggaca agtactttaa gaaccacaca     3480

agccccgacg tggacctggg agacattagc ggaatcaacg ccagcgtggt caacatccag     3540

aaagagattg accgcctgaa cgaggtggcc aagaatctga acgagagcct gatcgacctg     3600

caagaactgg gcaaatacga gcagtacatt aagtggccct ggtacatctg gctgggcttc     3660

attgccggac tgattgccat cgtgatggtc accattatgc tgtgctgcat gaccagttgc     3720

tgcagctgcc tgaagggatg ctgcagttgc ggaagctgct gcaagttcga cgaggatgat     3780

agcgagccag tgctgaaggg cgtcaagctg cactacacct ga                        3822


