                         SEQUENCE LISTING

<110>  Givaudan SA
<120>  ENZYME-MEDIATED PROCESS

<130>  31049 PCT

<160>  22    

<170>  PatentIn version 3.5

<210>  1
<211>  631
<212>  PRT
<213>  Alicyclobacillus acidocaldarius wt SHC enzyme

<400>  1

Met Ala Glu Gln Leu Val Glu Ala Pro Ala Tyr Ala Arg Thr Leu Asp 
1               5                   10                  15      


Arg Ala Val Glu Tyr Leu Leu Ser Cys Gln Lys Asp Glu Gly Tyr Trp 
            20                  25                  30          


Trp Gly Pro Leu Leu Ser Asn Val Thr Met Glu Ala Glu Tyr Val Leu 
        35                  40                  45              


Leu Cys His Ile Leu Asp Arg Val Asp Arg Asp Arg Met Glu Lys Ile 
    50                  55                  60                  


Arg Arg Tyr Leu Leu His Glu Gln Arg Glu Asp Gly Thr Trp Ala Leu 
65                  70                  75                  80  


Tyr Pro Gly Gly Pro Pro Asp Leu Asp Thr Thr Ile Glu Ala Tyr Val 
                85                  90                  95      


Ala Leu Lys Tyr Ile Gly Met Ser Arg Asp Glu Glu Pro Met Gln Lys 
            100                 105                 110         


Ala Leu Arg Phe Ile Gln Ser Gln Gly Gly Ile Glu Ser Ser Arg Val 
        115                 120                 125             


Phe Thr Arg Met Trp Leu Ala Leu Val Gly Glu Tyr Pro Trp Glu Lys 
    130                 135                 140                 


Val Pro Met Val Pro Pro Glu Ile Met Phe Leu Gly Lys Arg Met Pro 
145                 150                 155                 160 


Leu Asn Ile Tyr Glu Phe Gly Ser Trp Ala Arg Ala Thr Val Val Ala 
                165                 170                 175     


Leu Ser Ile Val Met Ser Arg Gln Pro Val Phe Pro Leu Pro Glu Arg 
            180                 185                 190         


Ala Arg Val Pro Glu Leu Tyr Glu Thr Asp Val Pro Pro Arg Arg Arg 
        195                 200                 205             


Gly Ala Lys Gly Gly Gly Gly Trp Ile Phe Asp Ala Leu Asp Arg Ala 
    210                 215                 220                 


Leu His Gly Tyr Gln Lys Leu Ser Val His Pro Phe Arg Arg Ala Ala 
225                 230                 235                 240 


Glu Ile Arg Ala Leu Asp Trp Leu Leu Glu Arg Gln Ala Gly Asp Gly 
                245                 250                 255     


Ser Trp Gly Gly Ile Gln Pro Pro Trp Phe Tyr Ala Leu Ile Ala Leu 
            260                 265                 270         


Lys Ile Leu Asp Met Thr Gln His Pro Ala Phe Ile Lys Gly Trp Glu 
        275                 280                 285             


Gly Leu Glu Leu Tyr Gly Val Glu Leu Asp Tyr Gly Gly Trp Met Phe 
    290                 295                 300                 


Gln Ala Ser Ile Ser Pro Val Trp Asp Thr Gly Leu Ala Val Leu Ala 
305                 310                 315                 320 


Leu Arg Ala Ala Gly Leu Pro Ala Asp His Asp Arg Leu Val Lys Ala 
                325                 330                 335     


Gly Glu Trp Leu Leu Asp Arg Gln Ile Thr Val Pro Gly Asp Trp Ala 
            340                 345                 350         


Val Lys Arg Pro Asn Leu Lys Pro Gly Gly Phe Ala Phe Gln Phe Asp 
        355                 360                 365             


Asn Val Tyr Tyr Pro Asp Val Asp Asp Thr Ala Val Val Val Trp Ala 
    370                 375                 380                 


Leu Asn Thr Leu Arg Leu Pro Asp Glu Arg Arg Arg Arg Asp Ala Met 
385                 390                 395                 400 


Thr Lys Gly Phe Arg Trp Ile Val Gly Met Gln Ser Ser Asn Gly Gly 
                405                 410                 415     


Trp Gly Ala Tyr Asp Val Asp Asn Thr Ser Asp Leu Pro Asn His Ile 
            420                 425                 430         


Pro Phe Cys Asp Phe Gly Glu Val Thr Asp Pro Pro Ser Glu Asp Val 
        435                 440                 445             


Thr Ala His Val Leu Glu Cys Phe Gly Ser Phe Gly Tyr Asp Asp Ala 
    450                 455                 460                 


Trp Lys Val Ile Arg Arg Ala Val Glu Tyr Leu Lys Arg Glu Gln Lys 
465                 470                 475                 480 


Pro Asp Gly Ser Trp Phe Gly Arg Trp Gly Val Asn Tyr Leu Tyr Gly 
                485                 490                 495     


Thr Gly Ala Val Val Ser Ala Leu Lys Ala Val Gly Ile Asp Thr Arg 
            500                 505                 510         


Glu Pro Tyr Ile Gln Lys Ala Leu Asp Trp Val Glu Gln His Gln Asn 
        515                 520                 525             


Pro Asp Gly Gly Trp Gly Glu Asp Cys Arg Ser Tyr Glu Asp Pro Ala 
    530                 535                 540                 


Tyr Ala Gly Lys Gly Ala Ser Thr Pro Ser Gln Thr Ala Trp Ala Leu 
545                 550                 555                 560 


Met Ala Leu Ile Ala Gly Gly Arg Ala Glu Ser Glu Ala Ala Arg Arg 
                565                 570                 575     


Gly Val Gln Tyr Leu Val Glu Thr Gln Arg Pro Asp Gly Gly Trp Asp 
            580                 585                 590         


Glu Pro Tyr Tyr Thr Gly Thr Gly Phe Pro Gly Asp Phe Tyr Leu Gly 
        595                 600                 605             


Tyr Thr Met Tyr Arg His Val Phe Pro Thr Leu Ala Leu Gly Arg Tyr 
    610                 615                 620                 


Lys Gln Ala Ile Glu Arg Arg 
625                 630     


<210>  2
<211>  1896
<212>  DNA
<213>  Alicyclobacillus acidocaldarius wt SHC enzyme

<400>  2
atggctgagc agttggtgga agcgccggcc tacgcgcgga cgctggatcg cgcggtggag       60

tatctcctct cctgccaaaa ggacgaaggc tactggtggg ggccgcttct gagcaacgtc      120

acgatggaag cggagtacgt cctcttgtgc cacattctcg atcgcgtcga tcgggatcgc      180

atggagaaga tccggcggta cctgttgcac gagcagcgcg aggacggcac gtgggccctg      240

tacccgggtg ggccgccgga cctcgacacg accatcgagg cgtacgtcgc gctcaagtat      300

atcggcatgt cgcgcgacga ggagccgatg cagaaggcgc tccggttcat tcagagccag      360

ggcgggatcg agtcgtcgcg cgtgttcacg cggatgtggc tggcgctggt gggagaatat      420

ccgtgggaga aggtgcccat ggtcccgccg gagatcatgt tcctcggcaa gcgcatgccg      480

ctcaacatct acgagtttgg ctcgtgggct cgggcgaccg tcgtggcgct ctcgattgtg      540

atgagccgcc agccggtgtt cccgctgccc gagcgggcgc gcgtgcccga gctgtacgag      600

accgacgtgc ctccgcgccg gcgcggtgcc aagggagggg gtgggtggat cttcgacgcg      660

ctcgaccggg cgctgcacgg gtatcagaag ctgtcggtgc acccgttccg ccgcgcggcc      720

gagatccgcg ccttggactg gttgctcgag cgccaggccg gagacggcag ctggggcggg      780

attcagccgc cttggtttta cgcgctcatc gcgctcaaga ttctcgacat gacgcagcat      840

ccggcgttca tcaagggctg ggaaggtcta gagctgtacg gcgtggagct ggattacgga      900

ggatggatgt ttcaggcttc catctcgccg gtgtgggaca cgggcctcgc cgtgctcgcg      960

ctgcgcgctg cggggcttcc ggccgatcac gaccgcttgg tcaaggcggg cgagtggctg     1020

ttggaccggc agatcacggt tccgggcgac tgggcggtga agcgcccgaa cctcaagccg     1080

ggcgggttcg cgttccagtt cgacaacgtg tactacccgg acgtggacga cacggccgtc     1140

gtggtgtggg cgctcaacac cctgcgcttg ccggacgagc gccgcaggcg ggacgccatg     1200

acgaagggat tccgctggat tgtcggcatg cagagctcga acggcggttg gggcgcctac     1260

gacgtcgaca acacgagcga tctcccgaac cacatcccgt tctgcgactt cggcgaagtg     1320

accgatccgc cgtcagagga cgtcaccgcc cacgtgctcg agtgtttcgg cagcttcggg     1380

tacgatgacg cctggaaggt catccggcgc gcggtggaat atctcaagcg ggagcagaag     1440

ccggacggca gctggttcgg tcgttggggc gtcaattacc tctacggcac gggcgcggtg     1500

gtgtcggcgc tgaaggcggt cgggatcgac acgcgcgagc cgtacattca aaaggcgctc     1560

gactgggtcg agcagcatca gaacccggac ggcggctggg gcgaggactg ccgctcgtac     1620

gaggatccgg cgtacgcggg taagggcgcg agcaccccgt cgcagacggc ctgggcgctg     1680

atggcgctca tcgcgggcgg cagggcggag tccgaggccg cgcgccgcgg cgtgcaatac     1740

ctcgtggaga cgcagcgccc ggacggcggc tgggatgagc cgtactacac cggcacgggc     1800

ttcccagggg atttctacct cggctacacc atgtaccgcc acgtgtttcc gacgctcgcg     1860

ctcggccgct acaagcaagc catcgagcgc aggtga                               1896


<210>  3
<211>  630
<212>  PRT
<213>  Artificial sequence

<220>
<223>  SHC enzyme variant #65 

<400>  3

Met Ala Glu Gln Leu Val Glu Ala Pro Ala Tyr Ala Arg Thr Leu Asp 
1               5                   10                  15      


Arg Ala Val Glu Tyr Leu Leu Ser Cys Gln Lys Asp Glu Gly Tyr Trp 
            20                  25                  30          


Trp Gly Pro Leu Leu Ser Asn Val Thr Met Glu Ala Glu Tyr Val Leu 
        35                  40                  45              


Leu Cys His Ile Leu Asp Arg Val Asp Arg Asp Arg Met Glu Lys Ile 
    50                  55                  60                  


Arg Arg Tyr Leu Leu His Glu Gln Arg Glu Asp Gly Thr Trp Ala Leu 
65                  70                  75                  80  


Tyr Pro Gly Gly Pro Pro Asp Leu Asp Thr Thr Ile Glu Ala Tyr Val 
                85                  90                  95      


Ala Leu Lys Tyr Ile Gly Met Ser Arg Asp Glu Glu Pro Met Gln Lys 
            100                 105                 110         


Ala Leu Arg Phe Ile Gln Ser Gln Gly Gly Ile Glu Ser Ser Arg Val 
        115                 120                 125             


Phe Thr Arg Arg Trp Leu Ala Leu Val Gly Glu Tyr Pro Trp Glu Lys 
    130                 135                 140                 


Val Pro Met Val Pro Pro Glu Ile Met Phe Leu Gly Lys Arg Met Pro 
145                 150                 155                 160 


Leu Asn Ile Tyr Glu Phe Gly Ser Trp Ala Arg Ala Thr Val Val Ala 
                165                 170                 175     


Leu Ser Ile Val Met Ser Arg Gln Pro Val Phe Pro Leu Pro Glu Arg 
            180                 185                 190         


Ala Arg Val Pro Glu Leu Tyr Glu Thr Asp Val Pro Pro Arg Arg Arg 
        195                 200                 205             


Gly Ala Lys Gly Gly Gly Gly Trp Ile Phe Asp Ala Leu Asp Arg Val 
    210                 215                 220                 


Leu His Gly Tyr Gln Lys Leu Ser Val His Pro Phe Arg Arg Ala Ala 
225                 230                 235                 240 


Glu Ile Arg Ala Leu Asp Trp Leu Leu Glu Arg Gln Ala Gly Asp Gly 
                245                 250                 255     


Ser Trp Gly Gly Ile Gln Pro Pro Trp Phe Tyr Ala Leu Ile Ala Leu 
            260                 265                 270         


Lys Ile Leu Asp Met Thr Gln His Pro Ala Phe Ile Lys Gly Trp Glu 
        275                 280                 285             


Gly Leu Glu Leu Tyr Gly Val Glu Leu Asp Tyr Gly Gly Trp Met Phe 
    290                 295                 300                 


Gln Ala Ser Ile Ser Pro Val Trp Asp Thr Gly Leu Ala Val Leu Ala 
305                 310                 315                 320 


Leu Arg Ala Ala Gly Leu Pro Ala Asp His Asp Arg Leu Val Lys Ala 
                325                 330                 335     


Gly Glu Trp Leu Leu Asp Arg Gln Ile Thr Val Pro Gly Asp Trp Ala 
            340                 345                 350         


Val Lys Arg Pro Asn Leu Lys Pro Gly Gly Phe Ala Phe Gln Phe Asp 
        355                 360                 365             


Asn Val Tyr Tyr Pro Asp Val Asp Asp Thr Ala Val Val Val Trp Ala 
    370                 375                 380                 


Leu Asn Thr Leu Arg Leu Pro Asp Glu Arg Arg Arg Arg Asp Ala Met 
385                 390                 395                 400 


Thr Lys Gly Phe Arg Trp Ile Val Gly Met Gln Ser Ser Asn Gly Gly 
                405                 410                 415     


Trp Gly Ala Tyr Asp Val Asp Asn Thr Ser Asp Leu Pro Asn His Thr 
            420                 425                 430         


Pro Phe Cys Asp Phe Gly Glu Val Thr Asp Pro Pro Ser Glu Asp Val 
        435                 440                 445             


Thr Ala His Val Leu Glu Cys Phe Gly Ser Phe Gly Tyr Asp Asp Ala 
    450                 455                 460                 


Trp Lys Val Ile Arg Arg Ala Val Glu Tyr Leu Lys Arg Glu Gln Lys 
465                 470                 475                 480 


Pro Asp Gly Ser Trp Phe Gly Arg Trp Gly Val Asn Tyr Leu Tyr Gly 
                485                 490                 495     


Thr Gly Ala Val Val Ser Ala Leu Lys Ala Val Gly Ile Asp Thr Arg 
            500                 505                 510         


Glu Pro Tyr Ile Gln Lys Ala Leu Asp Trp Val Glu Gln His Gln Asn 
        515                 520                 525             


Pro Asp Gly Gly Trp Gly Glu Asp Cys Arg Ser Tyr Glu Asp Pro Ala 
    530                 535                 540                 


Tyr Ala Gly Lys Gly Ala Ser Thr Pro Ser Gln Thr Thr Trp Ala Leu 
545                 550                 555                 560 


Met Ala Leu Ile Ala Gly Gly Arg Ala Glu Ser Glu Ala Ala Arg Arg 
                565                 570                 575     


Gly Val Gln Tyr Leu Val Glu Thr Gln Arg Pro Asp Gly Gly Trp Asp 
            580                 585                 590         


Glu Pro Tyr Tyr Thr Gly Thr Gly Phe Pro Gly Asp Phe Tyr Leu Gly 
        595                 600                 605             


Tyr Thr Met Tyr Ser His Val Phe Pro Thr Leu Ala Leu Gly Arg Tyr 
    610                 615                 620                 


Lys Gln Ala Ile Glu Arg 
625                 630 


<210>  4
<211>  1896
<212>  DNA
<213>  Artificial sequence

<220>
<223>  SHC enzyme variant #65

<400>  4
atggctgagc agttggtgga agctccggcc tacgcgcgga cgctggatcg cgcggtggag       60

tatctcctct cctgccaaaa ggacgaaggc tactggtggg ggccgcttct gagcaacgtc      120

acgatggaag cggagtacgt cctcttgtgc cacattctcg atcgcgtcga tcgggatcgc      180

atggagaaga tccggcggta cctgttgcac gagcagcgcg aggacggcac gtgggccctg      240

tacccgggtg ggccgccgga cctcgacacg accatcgagg cgtacgtcgc gctcaagtat      300

atcggcatgt cgcgcgacga ggagccgatg cagaaggcgc tccggttcat tcagagccag      360

ggcgggatcg agtcgtcgcg cgtgttcacg cggaggtggc tggcgctggt gggagaatat      420

ccgtgggaga aggtgcccat ggtcccgccg gagatcatgt tcctcggcaa gcgcatgccg      480

ctcaacatct acgagtttgg ctcgtgggct cgggcgaccg tcgtggcgct ctcgattgtg      540

atgagccgcc agccggtgtt cccgctgccc gagcgggcgc gcgtgcccga gctgtacgag      600

accgacgtgc ctccgcgccg gcgcggtgcc aagggagggg gtgggtggat cttcgacgcg      660

ctcgaccggg tgctgcacgg gtatcagaag ctgtcggtgc acccgttccg ccgcgcggcc      720

gagatccgcg ccttggactg gttgctcgag cgccaggccg gagacggcag ctggggcggg      780

attcagccgc cttggtttta cgcgctcatc gcgctcaaga ttctcgacat gacgcagcat      840

ccggcgttca tcaagggctg ggaaggtcta gagctgtacg gcgtggagct ggattacgga      900

ggatggatgt ttcaggcttc catctcgccg gtgtgggaca cgggcctcgc cgtgctcgcg      960

ctgcgcgctg cggggcttcc ggccgatcac gaccgcttgg tcaaggcggg cgagtggctg     1020

ttggaccggc agatcacggt tccgggcgac tgggcggtga agcgcccgaa cctcaagccg     1080

ggcgggttcg cgttccagtt cgacaacgtg tactacccgg acgtggacga cacggccgtc     1140

gtggtgtggg cgctcaacac cctgcgcttg ccggacgagc gccgcaggcg ggacgccatg     1200

acgaagggat tccgctggat tgtcggcatg cagagctcga acggcggttg gggcgcctac     1260

gacgtcgaca acacgagcga tctcccgaac cacaccccgt tctgcgactt cggcgaagtg     1320

accgatccgc cgtcagagga cgtcaccgcc cacgtgctcg agtgtttcgg cagcttcggg     1380

tacgatgacg cctggaaggt catccggcgc gcggtggaat atctcaagcg ggagcagaag     1440

ccggacggca gctggttcgg tcgttggggc gtcaattacc tctacggcac gggcgcggtg     1500

gtgtcggcgc tgaaggcggt cgggatcgac acgcgcgagc cgtacattca aaaggcgctc     1560

gactgggtcg agcagcatca gaacccggac ggcggctggg gcgaggactg ccgctcgtac     1620

gaggatccgg cgtacgcggg taagggcgcg agcaccccgt cgcagacgac ctgggcgctg     1680

atggcgctca tcgcgggcgg cagggcggag tccgaggccg cgcgccgcgg cgtgcaatac     1740

ctcgtggaga cgcagcgccc ggacggcggc tgggatgagc cgtactacac cggcacgggc     1800

ttcccagggg atttctacct cggctacacc atgtacagcc acgtgtttcc gacgctcgcg     1860

ctcggccgct acaagcaagc catcgagcgc aggtga                               1896


<210>  5
<211>  631
<212>  PRT
<213>  Artificial sequence

<220>
<223>  SHC enzyme variant #66

<400>  5

Met Ala Glu Gln Leu Val Glu Ala Pro Ala Tyr Ala Arg Thr Leu Asp 
1               5                   10                  15      


Arg Ala Val Glu Tyr Leu Leu Ser Cys Gln Lys Asp Glu Gly Tyr Trp 
            20                  25                  30          


Trp Gly Pro Leu Leu Ser Asn Val Thr Met Glu Ala Glu Tyr Val Leu 
        35                  40                  45              


Leu Cys His Ile Leu Asp Arg Val Asp Arg Asp Arg Met Glu Lys Ile 
    50                  55                  60                  


Arg Arg Tyr Leu Leu His Glu Gln Arg Glu Asp Gly Thr Trp Ala Leu 
65                  70                  75                  80  


His Pro Gly Gly Pro Pro Asp Leu Asp Thr Thr Ile Glu Ala Tyr Val 
                85                  90                  95      


Ala Leu Lys Tyr Ile Gly Met Ser Arg Asp Glu Glu Pro Met Gln Lys 
            100                 105                 110         


Ala Leu Arg Phe Ile Gln Ser Gln Gly Gly Ile Glu Ser Ser Arg Val 
        115                 120                 125             


Phe Thr Arg Arg Trp Leu Ala Leu Val Gly Glu Tyr Pro Trp Glu Lys 
    130                 135                 140                 


Val Pro Met Val Pro Pro Glu Ile Met Phe Leu Gly Lys Arg Met Pro 
145                 150                 155                 160 


Leu Asn Ile Tyr Glu Phe Gly Ser Trp Ala Arg Ala Thr Val Val Ala 
                165                 170                 175     


Leu Ser Ile Val Met Ser Arg Gln Pro Val Phe Pro Leu Pro Glu Arg 
            180                 185                 190         


Ala Arg Val Pro Glu Leu Tyr Glu Thr Asp Val Pro Pro Arg Arg Arg 
        195                 200                 205             


Gly Ala Lys Gly Gly Gly Gly Trp Ile Phe Asp Ala Leu Asp Arg Val 
    210                 215                 220                 


Leu His Gly Tyr Gln Lys Leu Ser Val His Pro Phe Arg Arg Ala Ala 
225                 230                 235                 240 


Glu Ile Arg Ala Leu Asp Trp Leu Leu Glu Arg Gln Ala Gly Asp Gly 
                245                 250                 255     


Ser Trp Gly Gly Ile Gln Pro Pro Trp Phe Tyr Ala Leu Ile Ala Leu 
            260                 265                 270         


Lys Ile Leu Asp Met Thr Gln His Pro Ala Phe Ile Lys Gly Trp Glu 
        275                 280                 285             


Gly Leu Glu Leu Tyr Gly Val Glu Leu Asp Tyr Gly Gly Trp Met Phe 
    290                 295                 300                 


Gln Ala Ser Ile Ser Pro Val Trp Asp Thr Gly Leu Ala Val Leu Ala 
305                 310                 315                 320 


Leu Arg Ala Ala Gly Leu Pro Ala Asp His Asp Arg Leu Val Lys Ala 
                325                 330                 335     


Gly Glu Trp Leu Leu Asp Arg Gln Ile Thr Val Pro Gly Asp Trp Ala 
            340                 345                 350         


Val Lys Arg Pro Asn Leu Lys Pro Gly Gly Phe Ala Phe Gln Phe Asp 
        355                 360                 365             


Asn Val Tyr Tyr Pro Asp Val Asp Asp Thr Ala Val Val Val Trp Ala 
    370                 375                 380                 


Leu Asn Thr Leu Arg Leu Pro Asp Glu Arg Arg Arg Arg Asp Ala Met 
385                 390                 395                 400 


Thr Lys Gly Phe Arg Trp Ile Val Gly Met Gln Ser Ser Asn Gly Gly 
                405                 410                 415     


Trp Gly Ala Tyr Asp Val Asp Asn Thr Ser Asp Leu Pro Asn His Thr 
            420                 425                 430         


Pro Phe Cys Asp Phe Gly Glu Val Thr Asp Pro Pro Ser Glu Asp Val 
        435                 440                 445             


Thr Ala His Val Leu Glu Cys Phe Gly Ser Phe Gly Tyr Asp Asp Ala 
    450                 455                 460                 


Trp Lys Val Ile Arg Arg Ala Val Glu Tyr Leu Lys Arg Glu Gln Lys 
465                 470                 475                 480 


Pro Asp Gly Ser Trp Phe Gly Arg Trp Gly Val Asn Tyr Leu Tyr Gly 
                485                 490                 495     


Thr Gly Ala Val Val Ser Ala Leu Lys Ala Val Gly Ile Asp Thr Arg 
            500                 505                 510         


Glu Pro Tyr Ile Gln Lys Ala Leu Asp Trp Val Glu Gln His Gln Asn 
        515                 520                 525             


Pro Asp Gly Gly Trp Gly Glu Asp Cys Arg Ser Tyr Glu Asp Pro Ala 
    530                 535                 540                 


Tyr Ala Gly Lys Gly Ala Ser Thr Pro Ser Gln Thr Thr Trp Ala Leu 
545                 550                 555                 560 


Met Ala Leu Ile Ala Gly Gly Arg Ala Glu Ser Glu Ala Ala Arg Arg 
                565                 570                 575     


Gly Val Gln Tyr Leu Val Glu Thr Gln Arg Pro Asp Gly Gly Trp Asp 
            580                 585                 590         


Glu Pro Tyr Tyr Thr Gly Thr Gly Phe Pro Gly Asp Phe Tyr Leu Gly 
        595                 600                 605             


Tyr Thr Met Tyr Ser His Val Phe Pro Thr Leu Ala Leu Gly Arg Tyr 
    610                 615                 620                 


Lys Gln Ala Ile Glu Arg Arg 
625                 630     


<210>  6
<211>  1896
<212>  DNA
<213>  Artificial sequence

<220>
<223>  SHC enzyme variant #66

<400>  6
atggctgagc agttggtgga agctccggcc tacgcgcgga cgctggatcg cgcggtggag       60

tatctcctct cctgccaaaa ggacgaaggc tactggtggg ggccgcttct gagcaacgtc      120

acgatggaag cggagtacgt cctcttgtgc cacattctcg atcgcgtcga tcgggatcgc      180

atggagaaga tccggcggta cctgttgcac gagcagcgcg aggacggcac gtgggccctg      240

cacccgggtg ggccgccgga cctcgacacg accatcgagg cgtacgtcgc gctcaagtat      300

atcggcatgt cgcgcgacga ggagccgatg cagaaggcgc tccggttcat tcagagccag      360

ggcgggatcg agtcgtcgcg cgtgttcacg cggaggtggc tggcgctggt gggagaatat      420

ccgtgggaga aggtgcccat ggtcccgccg gagatcatgt tcctcggcaa gcgcatgccg      480

ctcaacatct acgagtttgg ctcgtgggct cgggcgaccg tcgtggcgct ctcgattgtg      540

atgagccgcc agccggtgtt cccgctgccc gagcgggcgc gcgtgcccga gctgtacgag      600

accgacgtgc ctccgcgccg gcgcggtgcc aagggagggg gtgggtggat cttcgacgcg      660

ctcgaccggg tgctgcacgg gtatcagaag ctgtcggtgc acccgttccg ccgcgcggcc      720

gagatccgcg ccttggactg gttgctcgag cgccaggccg gagacggcag ctggggcggg      780

attcagccgc cttggtttta cgcgctcatc gcgctcaaga ttctcgacat gacgcagcat      840

ccggcgttca tcaagggctg ggaaggtcta gagctgtacg gcgtggagct ggattacgga      900

ggatggatgt ttcaggcttc catctcgccg gtgtgggaca cgggcctcgc cgtgctcgcg      960

ctgcgcgctg cggggcttcc ggccgatcac gaccgcttgg tcaaggcggg cgagtggctg     1020

ttggaccggc agatcacggt tccgggcgac tgggcggtga agcgcccgaa cctcaagccg     1080

ggcgggttcg cgttccagtt cgacaacgtg tactacccgg acgtggacga cacggccgtc     1140

gtggtgtggg cgctcaacac cctgcgcttg ccggacgagc gccgcaggcg ggacgccatg     1200

acgaagggat tccgctggat tgtcggcatg cagagctcga acggcggttg gggcgcctac     1260

gacgtcgaca acacgagcga tctcccgaac cacaccccgt tctgcgactt cggcgaagtg     1320

accgatccgc cgtcagagga cgtcaccgcc cacgtgctcg agtgtttcgg cagcttcggg     1380

tacgatgacg cctggaaggt catccggcgc gcggtggaat atctcaagcg ggagcagaag     1440

ccggacggca gctggttcgg tcgttggggc gtcaattacc tctacggcac gggcgcggtg     1500

gtgtcggcgc tgaaggcggt cgggatcgac acgcgcgagc cgtacattca aaaggcgctc     1560

gactgggtcg agcagcatca gaacccggac ggcggctggg gcgaggactg ccgctcgtac     1620

gaggatccgg cgtacgcggg taagggcgcg agcaccccgt cgcagacgac ctgggcgctg     1680

atggcgctca tcgcgggcgg cagggcggag tccgaggccg cgcgccgcgg cgtgcaatac     1740

ctcgtggaga cgcagcgccc ggacggcggc tgggatgagc cgtactacac cggcacgggc     1800

ttcccagggg atttctacct cggctacacc atgtacagcc acgtgtttcc gacgctcgcg     1860

ctcggccgct acaagcaagc catcgagcgc aggtga                               1896


<210>  7
<211>  631
<212>  PRT
<213>  Artificial sequence

<220>
<223>  SHC enzyme variant #90C7

<400>  7

Met Ala Glu Gln Leu Val Glu Ala Pro Ala Tyr Ala Arg Thr Leu Asp 
1               5                   10                  15      


Arg Ala Val Glu Tyr Leu Leu Ser Cys Gln Lys Asp Glu Gly Tyr Trp 
            20                  25                  30          


Trp Gly Pro Leu Leu Ser Asn Val Thr Met Glu Ala Glu Tyr Val Leu 
        35                  40                  45              


Leu Cys His Ile Leu Asp Arg Val Asp Arg Asp Arg Met Glu Lys Ile 
    50                  55                  60                  


Arg Arg Tyr Leu Leu His Glu Gln Arg Glu Asp Gly Thr Trp Ala Leu 
65                  70                  75                  80  


Tyr Pro Gly Gly Pro Pro Asp Leu Asp Ala Thr Ile Glu Ala Tyr Val 
                85                  90                  95      


Ala Leu Lys Tyr Ile Gly Met Ser Arg Asp Glu Glu Pro Met Gln Lys 
            100                 105                 110         


Ala Leu Arg Phe Ile Gln Ser Gln Gly Gly Ile Glu Ser Ser Arg Val 
        115                 120                 125             


Phe Thr Arg Arg Trp Leu Ala Leu Val Gly Glu Tyr Pro Trp Glu Lys 
    130                 135                 140                 


Val Pro Met Val Pro Pro Glu Ile Met Phe Leu Gly Lys Arg Met Pro 
145                 150                 155                 160 


Leu Asn Ile Tyr Glu Phe Gly Ser Trp Ala Arg Ala Thr Val Val Ala 
                165                 170                 175     


Leu Ser Ile Val Met Ser Arg Gln Pro Val Phe Pro Leu Pro Glu Arg 
            180                 185                 190         


Ala Arg Val Pro Glu Leu Tyr Glu Thr Asp Val Pro Pro Arg Arg Arg 
        195                 200                 205             


Gly Ala Lys Gly Gly Gly Gly Trp Ile Phe Asp Ala Leu Asp Arg Val 
    210                 215                 220                 


Leu His Gly Tyr Gln Lys Leu Ser Val His Pro Phe Arg Arg Ala Ala 
225                 230                 235                 240 


Glu Ile Arg Ala Leu Asp Trp Leu Leu Glu Arg Gln Ala Gly Asp Gly 
                245                 250                 255     


Ser Trp Gly Gly Ile Gln Pro Pro Trp Phe Tyr Ala Leu Ile Ala Leu 
            260                 265                 270         


Lys Ile Leu Asp Met Thr Gln His Pro Ala Phe Ile Lys Gly Trp Glu 
        275                 280                 285             


Gly Leu Glu Leu Tyr Gly Val Glu Leu Asp Tyr Gly Gly Trp Met Phe 
    290                 295                 300                 


Gln Ala Ser Ile Ser Pro Val Trp Asp Thr Gly Leu Ala Val Leu Ala 
305                 310                 315                 320 


Leu Arg Ala Ala Gly Leu Pro Ala Asp His Asp Arg Leu Val Lys Ala 
                325                 330                 335     


Gly Glu Trp Leu Leu Asp Arg Gln Ile Thr Val Pro Gly Asp Trp Ala 
            340                 345                 350         


Val Lys Arg Pro Asn Leu Lys Pro Gly Gly Phe Ala Phe Gln Phe Asp 
        355                 360                 365             


Asn Val Tyr Tyr Pro Asp Val Asp Asp Thr Ala Val Val Val Trp Ala 
    370                 375                 380                 


Leu Asn Thr Leu Arg Leu Pro Asp Glu Arg Arg Arg Arg Asp Ala Met 
385                 390                 395                 400 


Thr Lys Gly Phe Arg Trp Ile Val Gly Met Gln Ser Ser Asn Gly Gly 
                405                 410                 415     


Trp Gly Ala Tyr Asp Val Asp Asn Thr Ser Asp Leu Pro Asn His Thr 
            420                 425                 430         


Pro Phe Cys Asp Phe Gly Glu Val Thr Asp Pro Pro Ser Glu Asp Val 
        435                 440                 445             


Thr Ala His Val Leu Glu Cys Phe Gly Ser Phe Gly Tyr Asp Asp Ala 
    450                 455                 460                 


Trp Lys Val Ile Arg Arg Ala Val Glu Tyr Leu Lys Arg Glu Gln Lys 
465                 470                 475                 480 


Pro Asp Gly Ser Trp Phe Gly Arg Trp Gly Val Asn Tyr Leu Tyr Gly 
                485                 490                 495     


Thr Gly Ala Val Val Ser Ala Leu Lys Ala Val Gly Ile Asp Thr Arg 
            500                 505                 510         


Glu Pro Tyr Ile Gln Lys Ala Leu Asp Trp Val Glu Gln His Gln Asn 
        515                 520                 525             


Pro Asp Gly Gly Trp Gly Glu Asp Cys Arg Ser Tyr Glu Asp Pro Ala 
    530                 535                 540                 


Tyr Ala Gly Lys Gly Ala Ser Thr Pro Ser Gln Thr Ala Trp Ala Leu 
545                 550                 555                 560 


Met Ala Leu Ile Ala Gly Gly Arg Ala Glu Ser Glu Ala Ala Arg Arg 
                565                 570                 575     


Gly Val Gln Tyr Leu Val Glu Thr Gln Arg Pro Asp Gly Gly Trp Asp 
            580                 585                 590         


Glu Pro Tyr Tyr Thr Gly Thr Gly Phe Pro Gly Asp Phe Tyr Leu Gly 
        595                 600                 605             


Tyr Thr Met Tyr Ser His Val Phe Pro Thr Leu Ala Leu Gly Arg Tyr 
    610                 615                 620                 


Lys Gln Ala Ile Glu Arg Arg 
625                 630     


<210>  8
<211>  1896
<212>  DNA
<213>  Artificial sequence

<220>
<223>  SHC enzyme variant #90C7

<400>  8
atggctgagc agttggtgga agctccggcc tacgcgcgga cgctggatcg cgcggtggag       60

tatctcctct cctgccaaaa ggacgaaggc tactggtggg ggccgcttct gagcaacgtc      120

acgatggaag cggagtacgt cctcttgtgc cacattctcg atcgcgtcga tcgggatcgc      180

atggagaaga tccggcggta cctgttgcac gagcagcgcg aggacggcac gtgggccctg      240

tacccgggtg ggccgccgga cctcgacgcg accatcgagg cgtacgtcgc gctcaagtat      300

atcggcatgt cgcgcgacga ggagccgatg cagaaggcgc tccggttcat tcagagccag      360

ggcgggatcg agtcgtcgcg cgtgttcacg cggaggtggc tggcgctggt gggagaatat      420

ccgtgggaga aggtgcccat ggtcccgccg gagatcatgt tcctcggcaa gcgcatgccg      480

ctcaacatct acgagtttgg ctcgtgggct cgggcgaccg tcgtggcgct ctcgattgtg      540

atgagccgcc agccggtgtt cccgctgccc gagcgggcgc gcgtgcccga gctgtacgag      600

accgacgtgc ctccgcgccg gcgcggtgcc aagggagggg gtgggtggat cttcgacgcg      660

ctcgaccggg tgctgcacgg gtatcagaag ctgtcggtgc acccgttccg ccgcgcggcc      720

gagatccgcg ccttggactg gttgctcgag cgccaggccg gagacggcag ctggggcggg      780

attcagccgc cttggtttta cgcgctcatc gcgctcaaga ttctcgacat gacgcagcat      840

ccggcgttca tcaagggctg ggaaggtcta gagctgtacg gcgtggagct ggattacgga      900

ggatggatgt ttcaggcttc catctcgccg gtgtgggaca cgggcctcgc cgtgctcgcg      960

ctgcgcgctg cggggcttcc ggccgatcac gaccgcttgg tcaaggcggg cgagtggctg     1020

ttggaccggc agatcacggt tccgggcgac tgggcggtga agcgcccgaa cctcaagccg     1080

ggcgggttcg cgttccagtt cgacaacgtg tactacccgg acgtggacga cacggccgtc     1140

gtggtgtggg cgctcaacac cctgcgcttg ccggacgagc gccgcaggcg ggacgccatg     1200

acgaagggat tccgctggat tgtcggcatg cagagctcga acggcggttg gggcgcctac     1260

gacgtcgaca acacgagcga tctcccgaac cacaccccgt tctgcgactt cggcgaagtg     1320

accgatccgc cgtcagagga cgtcaccgcc cacgtgctcg agtgtttcgg cagcttcggg     1380

tacgatgacg cctggaaggt catccggcgc gcggtggaat atctcaagcg ggagcagaag     1440

ccggacggca gctggttcgg tcgttggggc gtcaattacc tctacggcac gggcgcggtg     1500

gtgtcggcgc tgaaggcggt cgggatcgac acgcgcgagc cgtacattca aaaggcgctc     1560

gactgggtcg agcagcatca gaacccggac ggcggctggg gcgaggactg ccgctcgtac     1620

gaggatccgg cgtacgcggg taagggcgcg agcaccccgt cgcagacggc ctgggcgctg     1680

atggcgctca tcgcgggcgg cagggcggag tccgaggccg cgcgccgcgg cgtgcaatac     1740

ctcgtggaga cgcagcgccc ggacggcggc tgggatgagc cgtactacac cggcacgggc     1800

ttcccagggg atttctacct cggctacacc atgtacagcc acgtgtttcc gacgctcgcg     1860

ctcggccgct acaagcaagc catcgagcgc aggtga                               1896


<210>  9
<211>  631
<212>  PRT
<213>  Artificial sequence

<220>
<223>  SHC enzyme variant #110B8

<400>  9

Met Ala Glu Gln Leu Val Glu Ala Pro Ala Tyr Ala Arg Thr Leu Asp 
1               5                   10                  15      


Arg Ala Val Glu Tyr Leu Leu Ser Cys Gln Lys Asp Glu Gly Tyr Trp 
            20                  25                  30          


Trp Gly Pro Leu Leu Ser Asn Val Thr Met Glu Ala Glu Tyr Val Leu 
        35                  40                  45              


Leu Cys His Ile Leu Asp Arg Val Asp Arg Asp Arg Met Glu Lys Ile 
    50                  55                  60                  


Arg Arg Tyr Leu Leu His Glu Gln Arg Glu Asp Gly Thr Trp Ala Leu 
65                  70                  75                  80  


His Pro Gly Gly Pro Pro Asp Leu Asp Thr Thr Ile Glu Ala Tyr Val 
                85                  90                  95      


Ala Leu Lys Tyr Ile Gly Met Ser Arg Asp Glu Glu Pro Met Gln Lys 
            100                 105                 110         


Ala Leu Arg Phe Ile Gln Ser Gln Gly Gly Ile Glu Ser Ser Arg Val 
        115                 120                 125             


Phe Thr Arg Arg Trp Leu Ala Leu Val Gly Glu Tyr Pro Trp Glu Lys 
    130                 135                 140                 


Val Pro Met Val Pro Pro Glu Ile Met Phe Leu Gly Lys Arg Met Pro 
145                 150                 155                 160 


Leu Asn Ile Tyr Glu Phe Gly Ser Trp Ala Arg Ala Thr Val Val Ala 
                165                 170                 175     


Leu Ser Ile Val Met Ser Arg Gln Pro Val Phe Pro Leu Pro Glu Arg 
            180                 185                 190         


Ala Arg Val Pro Glu Leu Tyr Glu Thr Asp Val Pro Pro Arg Arg Arg 
        195                 200                 205             


Gly Ala Lys Gly Gly Gly Gly Trp Ile Phe Asp Ala Leu Asp Arg Val 
    210                 215                 220                 


Leu His Gly Tyr Gln Lys Leu Ser Val His Pro Phe Arg Arg Ala Ala 
225                 230                 235                 240 


Glu Ile Arg Ala Leu Asp Trp Leu Leu Glu Arg Gln Ala Gly Asp Gly 
                245                 250                 255     


Ser Trp Gly Gly Ile Gln Pro Pro Trp Phe Tyr Ala Leu Ile Ala Leu 
            260                 265                 270         


Lys Ile Leu Asp Met Thr Gln His Pro Ala Phe Ile Lys Gly Trp Glu 
        275                 280                 285             


Gly Leu Glu Leu Tyr Gly Val Glu Leu Asp Tyr Gly Gly Trp Met Phe 
    290                 295                 300                 


Gln Ala Ser Ile Ser Pro Val Trp Asp Thr Gly Leu Ala Val Leu Ala 
305                 310                 315                 320 


Leu Arg Ala Ala Gly Leu Pro Ala Asp His Asp Arg Leu Val Lys Ala 
                325                 330                 335     


Gly Glu Trp Leu Leu Asp Arg Gln Ile Thr Val Pro Gly Asp Trp Ala 
            340                 345                 350         


Val Lys Arg Pro Asn Leu Lys Pro Gly Gly Phe Ala Phe Gln Phe Asp 
        355                 360                 365             


Asn Val Tyr Tyr Pro Asp Val Asp Asp Thr Ala Val Val Val Trp Ala 
    370                 375                 380                 


Leu Asn Thr Leu Arg Leu Pro Asp Glu Arg Arg Arg Arg Asp Ala Met 
385                 390                 395                 400 


Thr Lys Gly Phe Arg Trp Ile Val Gly Met Gln Ser Ser Asn Gly Gly 
                405                 410                 415     


Trp Gly Ala Tyr Asp Val Asp Asn Thr Ser Asp Leu Pro Asn Leu Thr 
            420                 425                 430         


Pro Phe Cys Asp Phe Gly Glu Val Thr Asp Pro Pro Ser Glu Asp Val 
        435                 440                 445             


Thr Ala His Val Leu Glu Cys Phe Gly Ser Phe Gly Tyr Asp Asp Ala 
    450                 455                 460                 


Trp Lys Val Ile Arg Arg Ala Val Glu Tyr Leu Lys Arg Glu Gln Lys 
465                 470                 475                 480 


Pro Asp Gly Ser Trp Phe Gly Arg Trp Gly Val Asn Tyr Leu Tyr Gly 
                485                 490                 495     


Thr Gly Ala Val Val Ser Ala Leu Lys Ala Val Gly Ile Asp Thr Arg 
            500                 505                 510         


Glu Pro Tyr Ile Gln Lys Ala Leu Asp Trp Val Glu Gln His Gln Asn 
        515                 520                 525             


Pro Asp Gly Gly Trp Gly Glu Asp Cys Arg Ser Tyr Glu Asp Pro Ala 
    530                 535                 540                 


Tyr Ala Gly Lys Gly Ala Ser Thr Pro Ser Gln Thr Thr Trp Ala Leu 
545                 550                 555                 560 


Met Ala Leu Ile Ala Gly Gly Arg Ala Glu Ser Glu Ala Ala Arg Arg 
                565                 570                 575     


Gly Val Gln Tyr Leu Val Glu Thr Gln Arg Pro Asp Gly Gly Trp Asp 
            580                 585                 590         


Glu Pro Tyr Tyr Thr Gly Thr Gly Phe Pro Gly Asp Phe Tyr Leu Gly 
        595                 600                 605             


Tyr Thr Met Tyr Arg His Val Phe Pro Thr Leu Ala Leu Gly Arg Tyr 
    610                 615                 620                 


Lys Gln Ala Ile Glu Arg Arg 
625                 630     


<210>  10
<211>  1896
<212>  DNA
<213>  Artificial sequence

<220>
<223>  SHC enzyme variant #110B8

<400>  10
atggctgagc agttggtgga agctccggcc tacgcgcgga cgctggatcg cgcggtggag       60

tatctcctct cctgccaaaa ggacgaaggc tactggtggg ggccgcttct gagcaacgtc      120

acgatggaag cggagtacgt cctcttgtgc cacattctcg atcgcgtcga tcgggatcgc      180

atggagaaga tccggcggta cctgttgcac gagcagcgcg aggacggcac gtgggccctg      240

cacccgggtg ggccgccgga cctcgacacg accatcgagg cgtacgtcgc gctcaagtat      300

atcggcatgt cgcgcgacga ggagccgatg cagaaggcgc tccggttcat tcagagccag      360

ggcgggatcg agtcgtcgcg cgtgttcacg cggaggtggc tggcgctggt gggagaatat      420

ccgtgggaga aggtgcccat ggtcccgccg gagatcatgt tcctcggcaa gcgcatgccg      480

ctcaacatct acgagtttgg ctcgtgggct cgggcgaccg tcgtggcgct ctcgattgtg      540

atgagccgcc agccggtgtt cccgctgccc gagcgggcgc gcgtgcccga gctgtacgag      600

accgacgtgc ctccgcgccg gcgcggtgcc aagggagggg gtgggtggat cttcgacgcg      660

ctcgaccggg tgctgcacgg gtatcagaag ctgtcggtgc acccgttccg ccgcgcggcc      720

gagatccgcg ccttggactg gttgctcgag cgccaggccg gagacggcag ctggggcggg      780

attcagccgc cttggtttta cgcgctcatc gcgctcaaga ttctcgacat gacgcagcat      840

ccggcgttca tcaagggctg ggaaggtcta gagctgtacg gcgtggagct ggattacgga      900

ggatggatgt ttcaggcttc catctcgccg gtgtgggaca cgggcctcgc cgtgctcgcg      960

ctgcgcgctg cggggcttcc ggccgatcac gaccgcttgg tcaaggcggg cgagtggctg     1020

ttggaccggc agatcacggt tccgggcgac tgggcggtga agcgcccgaa cctcaagccg     1080

ggcgggttcg cgttccagtt cgacaacgtg tactacccgg acgtggacga cacggccgtc     1140

gtggtgtggg cgctcaacac cctgcgcttg ccggacgagc gccgcaggcg ggacgccatg     1200

acgaagggat tccgctggat tgtcggcatg cagagctcga acggcggttg gggcgcctac     1260

gacgtcgaca acacgagcga tctcccgaac ctcaccccgt tctgcgactt cggcgaagtg     1320

accgatccgc cgtcagagga cgtcaccgcc cacgtgctcg agtgtttcgg cagcttcggg     1380

tacgatgacg cctggaaggt catccggcgc gcggtggaat atctcaagcg ggagcagaag     1440

ccggacggca gctggttcgg tcgttggggc gtcaattacc tctacggcac gggcgcggtg     1500

gtgtcggcgc tgaaggcggt cgggatcgac acgcgcgagc cgtacattca aaaggcgctc     1560

gactgggtcg agcagcatca gaacccggac ggcggctggg gcgaggactg ccgctcgtac     1620

gaggatccgg cgtacgcggg taagggcgcg agcaccccgt cgcagacgac ctgggcgctg     1680

atggcgctca tcgcgggcgg cagggcggag tccgaggccg cgcgccgcgg cgtgcaatac     1740

ctcgtggaga cgcagcgccc ggacggcggc tgggatgagc cgtactacac cggcacgggc     1800

ttcccagggg atttctacct cggctacacc atgtaccgcc acgtgtttcc gacgctcgcg     1860

ctcggccgct acaagcaagc catcgagcgc aggtga                               1896


<210>  11
<211>  631
<212>  PRT
<213>  Artificial sequence

<220>
<223>  SHC enzyme variant #115A7

<400>  11

Met Ala Glu Gln Leu Val Glu Ala Pro Ala Tyr Ala Arg Thr Leu Asp 
1               5                   10                  15      


Arg Ala Val Glu Tyr Leu Leu Ser Cys Gln Lys Asp Glu Gly Tyr Trp 
            20                  25                  30          


Trp Gly Pro Leu Leu Ser Asn Val Thr Met Glu Ala Glu Tyr Val Leu 
        35                  40                  45              


Leu Cys His Ile Leu Asp Arg Val Asp Arg Asp Arg Met Glu Lys Ile 
    50                  55                  60                  


Arg Arg Tyr Leu Leu His Glu Gln Arg Glu Asp Gly Thr Trp Ala Leu 
65                  70                  75                  80  


Tyr Pro Gly Gly Pro Pro Asp Leu Asp Thr Thr Ile Glu Ala Tyr Val 
                85                  90                  95      


Ala Leu Lys Tyr Ile Gly Met Ser Arg Asp Glu Glu Pro Met Gln Lys 
            100                 105                 110         


Ala Leu Arg Phe Ile Gln Ser Gln Gly Gly Ile Glu Ser Ser Arg Val 
        115                 120                 125             


Phe Thr Arg Arg Trp Leu Ala Leu Val Gly Glu Tyr Pro Trp Glu Lys 
    130                 135                 140                 


Val Pro Met Val Pro Pro Glu Ile Met Phe Leu Gly Lys Arg Met Pro 
145                 150                 155                 160 


Leu Asn Ile Tyr Glu Phe Gly Ser Trp Ala Arg Thr Thr Val Val Ala 
                165                 170                 175     


Leu Ser Ile Val Met Ser Arg Gln Pro Val Phe Pro Leu Pro Glu Arg 
            180                 185                 190         


Ala Arg Val Pro Glu Leu Tyr Glu Thr Asp Val Pro Pro Arg Arg Arg 
        195                 200                 205             


Gly Ala Lys Gly Gly Gly Gly Trp Ile Phe Asp Ala Leu Asp Arg Val 
    210                 215                 220                 


Leu His Gly Tyr Gln Lys Leu Ser Val His Pro Phe Arg Arg Ala Ala 
225                 230                 235                 240 


Glu Ile Arg Ala Leu Asp Trp Leu Leu Glu Arg Gln Ala Gly Asp Gly 
                245                 250                 255     


Ser Trp Gly Gly Ile Gln Pro Pro Trp Phe Tyr Ala Leu Ile Ala Leu 
            260                 265                 270         


Lys Ile Leu Asp Lys Thr Gln His Pro Ala Phe Ile Lys Gly Trp Glu 
        275                 280                 285             


Gly Leu Glu Leu Tyr Gly Val Glu Leu Asp Tyr Gly Gly Trp Met Phe 
    290                 295                 300                 


Gln Ala Ser Ile Ser Pro Val Trp Asp Thr Gly Leu Ala Val Leu Ala 
305                 310                 315                 320 


Leu Arg Ala Ala Gly Leu Pro Ala Asp His Asp Arg Leu Val Lys Ala 
                325                 330                 335     


Gly Glu Trp Leu Leu Asp Arg Gln Ile Thr Val Pro Gly Asp Trp Ala 
            340                 345                 350         


Val Lys Arg Pro Asn Leu Lys Pro Gly Gly Phe Ala Phe Gln Phe Asp 
        355                 360                 365             


Asn Val Tyr Tyr Pro Asp Val Asp Asp Thr Ala Val Val Val Trp Ala 
    370                 375                 380                 


Leu Asn Thr Leu Arg Leu Pro Asp Glu Arg Arg Arg Arg Asp Ala Met 
385                 390                 395                 400 


Thr Lys Gly Phe Arg Trp Ile Val Gly Met Gln Ser Ser Asn Gly Gly 
                405                 410                 415     


Trp Gly Ala Tyr Asp Val Asp Asn Thr Ser Asp Leu Pro Asn His Thr 
            420                 425                 430         


Pro Phe Cys Asp Phe Gly Glu Val Thr Asp Pro Pro Ser Glu Asp Val 
        435                 440                 445             


Thr Ala His Val Leu Glu Cys Phe Gly Ser Phe Gly Tyr Asp Asp Ala 
    450                 455                 460                 


Trp Lys Val Ile Arg Arg Ala Val Glu Tyr Leu Lys Arg Glu Gln Lys 
465                 470                 475                 480 


Pro Asp Gly Ser Trp Phe Gly Arg Trp Gly Val Asn Tyr Leu Tyr Gly 
                485                 490                 495     


Thr Gly Ala Val Val Ser Ala Leu Lys Ala Val Gly Ile Asp Thr Arg 
            500                 505                 510         


Glu Pro Tyr Ile Gln Lys Ala Leu Asp Trp Val Glu Gln His Gln Asn 
        515                 520                 525             


Pro Asp Gly Gly Trp Gly Glu Asp Cys Arg Ser Tyr Glu Asp Pro Ala 
    530                 535                 540                 


Tyr Ala Gly Lys Gly Ala Ser Thr Pro Ser Gln Thr Ala Trp Ala Leu 
545                 550                 555                 560 


Met Ala Leu Ile Ala Gly Gly Arg Ala Glu Ser Glu Ala Ala Arg Arg 
                565                 570                 575     


Gly Val Gln Tyr Leu Val Glu Thr Gln Arg Pro Asp Gly Gly Trp Asp 
            580                 585                 590         


Glu Pro Tyr Tyr Thr Gly Thr Gly Phe Pro Gly Asp Phe Tyr Leu Gly 
        595                 600                 605             


Tyr Thr Met Tyr Arg His Val Phe Pro Thr Leu Ala Leu Gly Arg Tyr 
    610                 615                 620                 


Lys Gln Ala Ile Glu Arg Arg 
625                 630     


<210>  12
<211>  1896
<212>  DNA
<213>  Artificial sequence

<220>
<223>  SHC enzyme variant #115A7

<400>  12
atggctgagc agttggtgga agctccggcc tacgcgcgga cgctggatcg cgcggtggag       60

tatctcctct cctgccaaaa ggacgaaggc tactggtggg ggccgcttct gagcaacgtc      120

acgatggaag cggagtacgt cctcttgtgc cacattctcg atcgcgtcga tcgggatcgc      180

atggagaaga tccggcggta cctgttgcac gagcagcgcg aggacggcac gtgggccctg      240

tacccgggtg ggccgccgga cctcgacacg accatcgagg cgtacgtcgc gctcaagtat      300

atcggcatgt cgcgcgacga ggagccgatg cagaaggcgc tccggttcat tcagagccag      360

ggcgggatcg agtcgtcgcg cgtgttcacg cggaggtggc tggcgctggt gggagaatat      420

ccgtgggaga aggtgcccat ggtcccgccg gagatcatgt tcctcggcaa gcgcatgccg      480

ctcaacatct acgagtttgg ctcgtgggct cggacgaccg tcgtggcgct ctcgattgtg      540

atgagccgcc agccggtgtt cccgctgccc gagcgggcgc gcgtgcccga gctgtacgag      600

accgacgtgc ctccgcgccg gcgcggtgcc aagggagggg gtgggtggat cttcgacgcg      660

ctcgaccggg tgctgcacgg gtatcagaag ctgtcggtgc acccgttccg ccgcgcggcc      720

gagatccgcg ccttggactg gttgctcgag cgccaggccg gagacggcag ctggggcggg      780

attcagccgc cttggtttta cgcgctcatc gcgctcaaga ttctcgacaa gacgcagcat      840

ccggcgttca tcaagggctg ggaaggtcta gagctgtacg gcgtggagct ggattacgga      900

ggatggatgt ttcaggcttc catctcgccg gtgtgggaca cgggcctcgc cgtgctcgcg      960

ctgcgcgctg cggggcttcc ggccgatcac gaccgcttgg tcaaggcggg cgagtggctg     1020

ttggaccggc agatcacggt tccgggcgac tgggcggtga agcgcccgaa cctcaagccg     1080

ggcgggttcg cgttccagtt cgacaacgtg tactacccgg acgtggacga cacggccgtc     1140

gtggtgtggg cgctcaacac cctgcgcttg ccggacgagc gccgcaggcg ggacgccatg     1200

acgaagggat tccgctggat tgtcggcatg cagagctcga acggcggttg gggcgcctac     1260

gacgtcgaca acacgagcga tctcccgaac cacaccccgt tctgcgactt cggcgaagtg     1320

accgatccgc cgtcagagga cgtcaccgcc cacgtgctcg agtgtttcgg cagcttcggg     1380

tacgatgacg cctggaaggt catccggcgc gcggtggaat atctcaagcg ggagcagaag     1440

ccggacggca gctggttcgg tcgttggggc gtcaattacc tctacggcac gggcgcggtg     1500

gtgtcggcgc tgaaggcggt cgggatcgac acgcgcgagc cgtacattca aaaggcgctc     1560

gactgggtcg agcagcatca gaacccggac ggcggctggg gcgaggactg ccgctcgtac     1620

gaggatccgg cgtacgcggg taagggcgcg agcaccccgt cgcagacggc ctgggcgctg     1680

atggcgctca tcgcgggcgg cagggcggag tccgaggccg cgcgccgcgg cgtgcaatac     1740

ctcgtggaga cgcagcgccc ggacggcggc tgggatgagc cgtactacac cggcacgggc     1800

ttcccagggg atttctacct cggctacacc atgtaccgcc acgtgtttcc gacgctcgcg     1860

ctcggccgct acaagcaagc catcgagcgc aggtga                               1896


<210>  13
<211>  631
<212>  PRT
<213>  Artificial sequence

<220>
<223>  SHC enzyme variant #215G2

<400>  13

Met Ala Glu Gln Leu Val Glu Ala Pro Ala Tyr Ala Arg Thr Leu Asp 
1               5                   10                  15      


Arg Ala Val Glu Tyr Leu Leu Ser Cys Gln Lys Asp Glu Gly Tyr Trp 
            20                  25                  30          


Trp Gly Pro Leu Leu Ser Asn Val Thr Met Glu Ala Glu Tyr Val Leu 
        35                  40                  45              


Leu Cys His Ile Leu Asp Arg Val Asp Arg Asp Arg Met Glu Lys Ile 
    50                  55                  60                  


Arg Arg Tyr Leu Leu His Glu Gln Arg Glu Asp Gly Thr Trp Ala Leu 
65                  70                  75                  80  


Tyr Pro Gly Gly Pro Pro Asp Leu Asp Thr Thr Ile Glu Ala Tyr Val 
                85                  90                  95      


Ala Leu Lys Tyr Ile Gly Met Ser Arg Asp Glu Glu Pro Met Gln Lys 
            100                 105                 110         


Ala Leu Arg Phe Ile Gln Ser Gln Gly Gly Ile Glu Ser Ser Arg Val 
        115                 120                 125             


Phe Thr Arg Arg Trp Leu Ala Leu Val Gly Glu Tyr Pro Trp Glu Lys 
    130                 135                 140                 


Val Pro Met Val Pro Pro Glu Ile Met Phe Leu Gly Lys Arg Met Pro 
145                 150                 155                 160 


Leu Asn Ile Tyr Glu Phe Gly Ser Trp Ala Arg Ala Thr Val Val Ala 
                165                 170                 175     


Leu Ser Ile Val Met Ser Arg Gln Pro Val Phe Pro Leu Pro Glu Arg 
            180                 185                 190         


Ala Arg Val Pro Glu Leu Tyr Glu Thr Asp Val Pro Pro Arg Arg Arg 
        195                 200                 205             


Gly Ala Lys Gly Gly Gly Gly Trp Ile Phe Asp Ala Leu Asp Arg Val 
    210                 215                 220                 


Leu His Gly Tyr Gln Lys Leu Ser Val His Pro Phe Arg Arg Ala Ala 
225                 230                 235                 240 


Glu Ile Arg Ala Leu Asp Trp Leu Leu Glu Arg Gln Ala Gly Asp Gly 
                245                 250                 255     


Ser Trp Gly Gly Ile Gln Pro Pro Trp Phe Tyr Ala Leu Ile Ala Leu 
            260                 265                 270         


Lys Ile Leu Asp Met Thr Gln His Pro Ala Phe Ile Lys Gly Trp Glu 
        275                 280                 285             


Gly Leu Glu Leu Tyr Gly Val Glu Leu Asp Tyr Gly Gly Trp Met Phe 
    290                 295                 300                 


Gln Ala Ser Ile Ser Pro Val Trp Asp Thr Gly Leu Ala Val Leu Ala 
305                 310                 315                 320 


Leu Arg Ala Ala Gly Leu Pro Ala Asp His Asp Arg Leu Val Lys Ala 
                325                 330                 335     


Gly Glu Trp Leu Leu Asp Arg Gln Ile Thr Val Pro Gly Asp Trp Ala 
            340                 345                 350         


Val Lys Arg Pro Asn Leu Lys Pro Gly Gly Phe Ala Phe Gln Phe Asp 
        355                 360                 365             


Asn Val Tyr Tyr Pro Asp Val Asp Asp Thr Ala Val Val Val Trp Ala 
    370                 375                 380                 


Leu Asn Thr Leu Arg Leu Pro Asp Glu Arg Arg Arg Arg Asp Ala Met 
385                 390                 395                 400 


Thr Lys Gly Phe Arg Trp Ile Val Gly Met Gln Ser Ser Asn Gly Gly 
                405                 410                 415     


Trp Gly Ala Tyr Asp Val Asp Asn Thr Ser Asp Leu Pro Asn His Thr 
            420                 425                 430         


Pro Phe Cys Asp Phe Gly Glu Val Thr Asp Pro Pro Ser Glu Asp Val 
        435                 440                 445             


Thr Ala His Val Leu Glu Cys Phe Gly Ser Phe Gly Tyr Asp Asp Ala 
    450                 455                 460                 


Trp Lys Val Ile Arg Arg Ala Val Glu Tyr Leu Lys Arg Glu Gln Lys 
465                 470                 475                 480 


Pro Asp Gly Ser Trp Phe Gly Arg Trp Gly Val Asn Tyr Leu Tyr Gly 
                485                 490                 495     


Thr Gly Ala Val Val Ser Ala Leu Lys Ala Val Gly Ile Asp Thr Arg 
            500                 505                 510         


Glu Pro Tyr Ile Gln Lys Ala Leu Asp Trp Val Glu Gln His Gln Asn 
        515                 520                 525             


Pro Asp Gly Gly Trp Gly Glu Asp Cys Arg Ser Tyr Glu Asp Pro Ala 
    530                 535                 540                 


Tyr Ala Gly Lys Gly Ala Ser Thr Pro Ser Gln Thr Ala Trp Ala Leu 
545                 550                 555                 560 


Met Ala Leu Ile Ala Gly Gly Arg Ala Glu Ser Glu Ala Ala Arg Arg 
                565                 570                 575     


Gly Val Gln Tyr Leu Val Glu Thr Gln Arg Pro Asp Gly Gly Trp Asp 
            580                 585                 590         


Glu Pro Tyr Tyr Thr Gly Thr Gly Phe Pro Gly Asp Phe Tyr Leu Gly 
        595                 600                 605             


Tyr Thr Met Tyr Arg His Val Phe Pro Thr Leu Ala Leu Gly Arg Tyr 
    610                 615                 620                 


Lys Gln Ala Ile Glu Arg Arg 
625                 630     


<210>  14
<211>  1896
<212>  DNA
<213>  Artificial sequence

<220>
<223>  SHC enzyme variant #215G2

<400>  14
atggctgagc agttggtgga agctccggcc tacgcgcgga cgctggatcg cgcggtggag       60

tatctcctct cctgccaaaa ggacgaaggc tactggtggg ggccgcttct gagcaacgtc      120

acgatggaag cggagtacgt cctcttgtgc cacattctcg atcgcgtcga tcgggatcgc      180

atggagaaga tccggcggta cctgttgcac gagcagcgcg aggacggcac gtgggccctg      240

tacccgggtg ggccgccgga cctcgacacg accatcgagg cgtacgtcgc gctcaagtat      300

atcggcatgt cgcgcgacga ggagccgatg cagaaggcgc tccggttcat tcagagccag      360

ggcgggatcg agtcgtcgcg cgtgttcacg cggaggtggc tggcgctggt gggagaatat      420

ccgtgggaga aggtgcccat ggtcccgccg gagatcatgt tcctcggcaa gcgcatgccg      480

ctcaacatct acgagtttgg ctcgtgggct cgggcgaccg tcgtggcgct ctcgattgtg      540

atgagccgcc agccggtgtt cccgctgccc gagcgggcgc gcgtgcccga gctgtacgag      600

accgacgtgc ctccgcgccg gcgcggtgcc aagggagggg gtgggtggat cttcgacgcg      660

ctcgaccggg tgctgcacgg gtatcagaag ctgtcggtgc acccgttccg ccgcgcggcc      720

gagatccgcg ccttggactg gttgctcgag cgccaggccg gagacggcag ctggggcggg      780

attcagccgc cttggtttta cgcgctcatc gcgctcaaga ttctcgacat gacgcagcat      840

ccggcgttca tcaagggctg ggaaggtcta gagctgtacg gcgtggagct ggattacgga      900

ggatggatgt ttcaggcttc catctcgccg gtgtgggaca cgggcctcgc cgtgctcgcg      960

ctgcgcgctg cggggcttcc ggccgatcac gaccgcttgg tcaaggcggg cgagtggctg     1020

ttggaccggc agatcacggt tccgggcgac tgggcggtga agcgcccgaa cctcaagccg     1080

ggcgggttcg cgttccagtt cgacaacgtg tactacccgg acgtggacga cacggccgtc     1140

gtggtgtggg cgctcaacac cctgcgcttg ccggacgagc gccgcaggcg ggacgccatg     1200

acgaagggat tccgctggat tgtcggcatg cagagctcga acggcggttg gggcgcctac     1260

gacgtcgaca acacgagcga tctcccgaac cacaccccgt tctgcgactt cggcgaagtg     1320

accgatccgc cgtcagagga cgtcaccgcc cacgtgctcg agtgtttcgg cagcttcggg     1380

tacgatgacg cctggaaggt catccggcgc gcggtggaat atctcaagcg ggagcagaag     1440

ccggacggca gctggttcgg tcgttggggc gtcaattacc tctacggcac gggcgcggtg     1500

gtgtcggcgc tgaaggcggt cgggatcgac acgcgcgagc cgtacattca aaaggcgctc     1560

gactgggtcg agcagcatca gaacccggac ggcggctggg gcgaggactg ccgctcgtac     1620

gaggatccgg cgtacgcggg taagggcgcg agcaccccgt cgcagacggc ctgggcgctg     1680

atggcgctca tcgcgggcgg cagggcggag tccgaggccg cgcgccgcgg cgtgcaatac     1740

ctcgtggaga cgcagcgccc ggacggcggc tgggatgagc cgtactacac cggcacgggc     1800

ttcccagggg atttctacct cggctacacc atgtaccgcc acgtgtttcc gacgctcgcg     1860

ctcggccgct acaagcaagc catcgagcgc aggtga                               1896


<210>  15
<211>  608
<212>  PRT
<213>  Zymomonas mobilis wt SHC enzyme

<400>  15

Met Gly Ile Asp Arg Met Asn Ser Leu Ser Arg Leu Leu Met Lys Lys 
1               5                   10                  15      


Ile Phe Gly Ala Glu Lys Thr Ser Tyr Lys Pro Ala Ser Asp Thr Ile 
            20                  25                  30          


Ile Gly Thr Asp Thr Leu Lys Arg Pro Asn Arg Arg Pro Glu Pro Thr 
        35                  40                  45              


Ala Lys Val Asp Lys Thr Ile Phe Lys Thr Met Gly Asn Ser Leu Asn 
    50                  55                  60                  


Asn Thr Leu Val Ser Ala Cys Asp Trp Leu Ile Gly Gln Gln Lys Pro 
65                  70                  75                  80  


Asp Gly His Trp Val Gly Ala Val Glu Ser Asn Ala Ser Met Glu Ala 
                85                  90                  95      


Glu Trp Cys Leu Ala Leu Trp Phe Leu Gly Leu Glu Asp His Pro Leu 
            100                 105                 110         


Arg Pro Arg Leu Gly Asn Ala Leu Leu Glu Met Gln Arg Glu Asp Gly 
        115                 120                 125             


Ser Trp Gly Val Tyr Phe Gly Ala Gly Asn Gly Asp Ile Asn Ala Thr 
    130                 135                 140                 


Val Glu Ala Tyr Ala Ala Leu Arg Ser Leu Gly Tyr Ser Ala Asp Asn 
145                 150                 155                 160 


Pro Val Leu Lys Lys Ala Ala Ala Trp Ile Ala Glu Lys Gly Gly Leu 
                165                 170                 175     


Lys Asn Ile Arg Val Phe Thr Arg Tyr Trp Leu Ala Leu Ile Gly Glu 
            180                 185                 190         


Trp Pro Trp Glu Lys Thr Pro Asn Leu Pro Pro Glu Ile Ile Trp Phe 
        195                 200                 205             


Pro Asp Asn Phe Val Phe Ser Ile Tyr Asn Phe Ala Gln Trp Ala Arg 
    210                 215                 220                 


Ala Thr Met Val Pro Ile Ala Ile Leu Ser Ala Arg Arg Pro Ser Arg 
225                 230                 235                 240 


Pro Leu Arg Pro Gln Asp Arg Leu Asp Glu Leu Phe Pro Glu Gly Arg 
                245                 250                 255     


Ala Arg Phe Asp Tyr Glu Leu Pro Lys Lys Glu Gly Ile Asp Leu Trp 
            260                 265                 270         


Ser Gln Phe Phe Arg Thr Thr Asp Arg Gly Leu His Trp Val Gln Ser 
        275                 280                 285             


Asn Leu Leu Lys Arg Asn Ser Leu Arg Glu Ala Ala Ile Arg His Val 
    290                 295                 300                 


Leu Glu Trp Ile Ile Arg His Gln Asp Ala Asp Gly Gly Trp Gly Gly 
305                 310                 315                 320 


Ile Gln Pro Pro Trp Val Tyr Gly Leu Met Ala Leu His Gly Glu Gly 
                325                 330                 335     


Tyr Gln Leu Tyr His Pro Val Met Ala Lys Ala Leu Ser Ala Leu Asp 
            340                 345                 350         


Asp Pro Gly Trp Arg His Asp Arg Gly Glu Ser Ser Trp Ile Gln Ala 
        355                 360                 365             


Thr Asn Ser Pro Val Trp Asp Thr Met Leu Ala Leu Met Ala Leu Lys 
    370                 375                 380                 


Asp Ala Lys Ala Glu Asp Arg Phe Thr Pro Glu Met Asp Lys Ala Ala 
385                 390                 395                 400 


Asp Trp Leu Leu Ala Arg Gln Val Lys Val Lys Gly Asp Trp Ser Ile 
                405                 410                 415     


Lys Leu Pro Asp Val Glu Pro Gly Gly Trp Ala Phe Glu Tyr Ala Asn 
            420                 425                 430         


Asp Arg Tyr Pro Asp Thr Asp Asp Thr Ala Val Ala Leu Ile Ala Leu 
        435                 440                 445             


Ser Ser Tyr Arg Asp Lys Glu Glu Trp Gln Lys Lys Gly Val Glu Asp 
    450                 455                 460                 


Ala Ile Thr Arg Gly Val Asn Trp Leu Ile Ala Met Gln Ser Glu Cys 
465                 470                 475                 480 


Gly Gly Trp Gly Ala Phe Asp Lys Asp Asn Asn Arg Ser Ile Leu Ser 
                485                 490                 495     


Lys Ile Pro Phe Cys Asp Phe Gly Glu Ser Ile Asp Pro Pro Ser Val 
            500                 505                 510         


Asp Val Thr Ala His Val Leu Glu Ala Phe Gly Thr Leu Gly Leu Ser 
        515                 520                 525             


Arg Asp Met Pro Val Ile Gln Lys Ala Ile Asp Tyr Val Arg Ser Glu 
    530                 535                 540                 


Gln Glu Ala Glu Gly Ala Trp Phe Gly Arg Trp Gly Val Asn Tyr Ile 
545                 550                 555                 560 


Tyr Gly Thr Gly Ala Val Leu Pro Ala Leu Ala Ala Ile Gly Glu Asp 
                565                 570                 575     


Met Thr Gln Pro Tyr Ile Thr Lys Ala Cys Asp Trp Leu Val Ala His 
            580                 585                 590         


Gln Gln Glu Asp Gly Gly Trp Gly Glu Ser Cys Ser Ser Tyr Met Glu 
        595                 600                 605             


<210>  16
<211>  658
<212>  PRT
<213>  Zymomonas mobilis wt SHC enzyme

<400>  16

Met Thr Val Ser Thr Ser Ser Ala Phe His His Ser Pro Leu Ser Asp 
1               5                   10                  15      


Asp Val Glu Pro Ile Ile Gln Lys Ala Thr Arg Ala Leu Leu Glu Lys 
            20                  25                  30          


Gln Gln Gln Asp Gly His Trp Val Phe Glu Leu Glu Ala Asp Ala Thr 
        35                  40                  45              


Ile Pro Ala Glu Tyr Ile Leu Leu Lys His Tyr Leu Gly Glu Pro Glu 
    50                  55                  60                  


Asp Leu Glu Ile Glu Ala Lys Ile Gly Arg Tyr Leu Arg Arg Ile Gln 
65                  70                  75                  80  


Gly Glu His Gly Gly Trp Ser Leu Phe Tyr Gly Gly Asp Leu Asp Leu 
                85                  90                  95      


Ser Ala Thr Val Lys Ala Tyr Phe Ala Leu Lys Met Ile Gly Asp Ser 
            100                 105                 110         


Pro Asp Ala Pro His Met Leu Arg Ala Arg Asn Glu Ile Leu Ala Arg 
        115                 120                 125             


Gly Gly Ala Met Arg Ala Asn Val Phe Thr Arg Ile Gln Leu Ala Leu 
    130                 135                 140                 


Phe Gly Ala Met Ser Trp Glu His Val Pro Gln Met Pro Val Glu Leu 
145                 150                 155                 160 


Met Leu Met Pro Glu Trp Phe Pro Val His Ile Asn Lys Met Ala Tyr 
                165                 170                 175     


Trp Ala Arg Thr Val Leu Val Pro Leu Leu Val Leu Gln Ala Leu Lys 
            180                 185                 190         


Pro Val Ala Arg Asn Arg Arg Gly Ile Leu Val Asp Glu Leu Phe Val 
        195                 200                 205             


Pro Asp Val Leu Pro Thr Leu Gln Glu Ser Gly Asp Pro Ile Trp Arg 
    210                 215                 220                 


Arg Phe Phe Ser Ala Leu Asp Lys Val Leu His Lys Val Glu Pro Tyr 
225                 230                 235                 240 


Trp Pro Lys Asn Met Arg Ala Lys Ala Ile His Ser Cys Val His Phe 
                245                 250                 255     


Val Thr Glu Arg Leu Asn Gly Glu Asp Gly Leu Gly Ala Ile Tyr Pro 
            260                 265                 270         


Ala Ile Ala Asn Ser Val Met Met Tyr Asp Ala Leu Gly Tyr Pro Glu 
        275                 280                 285             


Asn His Pro Glu Arg Ala Ile Ala Arg Arg Ala Val Glu Lys Leu Met 
    290                 295                 300                 


Val Leu Asp Gly Thr Glu Asp Gln Gly Asp Lys Glu Val Tyr Cys Gln 
305                 310                 315                 320 


Pro Cys Leu Ser Pro Ile Trp Asp Thr Ala Leu Val Ala His Ala Met 
                325                 330                 335     


Leu Glu Val Gly Gly Asp Glu Ala Glu Lys Ser Ala Ile Ser Ala Leu 
            340                 345                 350         


Ser Trp Leu Lys Pro Gln Gln Ile Leu Asp Val Lys Gly Asp Trp Ala 
        355                 360                 365             


Trp Arg Arg Pro Asp Leu Arg Pro Gly Gly Trp Ala Phe Gln Tyr Arg 
    370                 375                 380                 


Asn Asp Tyr Tyr Pro Asp Val Asp Asp Thr Ala Val Val Thr Met Ala 
385                 390                 395                 400 


Met Asp Arg Ala Ala Lys Leu Ser Asp Leu His Asp Asp Phe Glu Glu 
                405                 410                 415     


Ser Lys Ala Arg Ala Met Glu Trp Thr Ile Gly Met Gln Ser Asp Asn 
            420                 425                 430         


Gly Gly Trp Gly Ala Phe Asp Ala Asn Asn Ser Tyr Thr Tyr Leu Asn 
        435                 440                 445             


Asn Ile Pro Phe Ala Asp His Gly Ala Leu Leu Asp Pro Pro Thr Val 
    450                 455                 460                 


Asp Val Ser Ala Arg Cys Val Ser Met Met Ala Gln Ala Gly Ile Ser 
465                 470                 475                 480 


Ile Thr Asp Pro Lys Met Lys Ala Ala Val Asp Tyr Leu Leu Lys Glu 
                485                 490                 495     


Gln Glu Glu Asp Gly Ser Trp Phe Gly Arg Trp Gly Val Asn Tyr Ile 
            500                 505                 510         


Tyr Gly Thr Trp Ser Ala Leu Cys Ala Leu Asn Val Ala Ala Leu Pro 
        515                 520                 525             


His Asp His Leu Ala Val Gln Lys Ala Val Ala Trp Leu Lys Thr Ile 
    530                 535                 540                 


Gln Asn Glu Asp Gly Gly Trp Gly Glu Asn Cys Asp Ser Tyr Ala Leu 
545                 550                 555                 560 


Asp Tyr Ser Gly Tyr Glu Pro Met Asp Ser Thr Ala Ser Gln Thr Ala 
                565                 570                 575     


Trp Ala Leu Leu Gly Leu Met Ala Val Gly Glu Ala Asn Ser Glu Ala 
            580                 585                 590         


Val Thr Lys Gly Ile Asn Trp Leu Ala Gln Asn Gln Asp Glu Glu Gly 
        595                 600                 605             


Leu Trp Lys Glu Asp Tyr Tyr Ser Gly Gly Gly Phe Pro Arg Val Phe 
    610                 615                 620                 


Tyr Leu Arg Tyr His Gly Tyr Ser Lys Tyr Phe Pro Leu Trp Ala Leu 
625                 630                 635                 640 


Ala Arg Tyr Arg Asn Leu Lys Lys Ala Asn Gln Pro Ile Val His Tyr 
                645                 650                 655     


Gly Met 
        


<210>  17
<211>  684
<212>  PRT
<213>  Bradyrhizobium japonicum wt SHC enzyme

<400>  17

Met Thr Val Thr Ser Ser Ala Ser Ala Arg Ala Thr Arg Asp Pro Gly 
1               5                   10                  15      


Asn Tyr Gln Thr Ala Leu Gln Ser Thr Val Arg Ala Ala Ala Asp Trp 
            20                  25                  30          


Leu Ile Ala Asn Gln Lys Pro Asp Gly His Trp Val Gly Arg Ala Glu 
        35                  40                  45              


Ser Asn Ala Cys Met Glu Ala Gln Trp Cys Leu Ala Leu Trp Phe Met 
    50                  55                  60                  


Gly Leu Glu Asp His Pro Leu Arg Lys Arg Leu Gly Gln Ser Leu Leu 
65                  70                  75                  80  


Asp Ser Gln Arg Pro Asp Gly Ala Trp Gln Val Tyr Phe Gly Ala Pro 
                85                  90                  95      


Asn Gly Asp Ile Asn Ala Thr Val Glu Ala Tyr Ala Ala Leu Arg Ser 
            100                 105                 110         


Leu Gly Phe Arg Asp Asp Glu Pro Ala Val Arg Arg Ala Arg Glu Trp 
        115                 120                 125             


Ile Glu Ala Lys Gly Gly Leu Arg Asn Ile Arg Val Phe Thr Arg Tyr 
    130                 135                 140                 


Trp Leu Ala Leu Ile Gly Glu Trp Pro Trp Glu Lys Thr Pro Asn Ile 
145                 150                 155                 160 


Pro Pro Glu Val Ile Trp Phe Pro Leu Trp Phe Pro Phe Ser Ile Tyr 
                165                 170                 175     


Asn Phe Ala Gln Trp Ala Arg Ala Thr Leu Met Pro Ile Ala Val Leu 
            180                 185                 190         


Ser Ala Arg Arg Pro Ser Arg Pro Leu Pro Pro Glu Asn Arg Leu Asp 
        195                 200                 205             


Ala Leu Phe Pro His Gly Arg Lys Ala Phe Asp Tyr Glu Leu Pro Val 
    210                 215                 220                 


Lys Ala Gly Ala Gly Gly Trp Asp Arg Phe Phe Arg Gly Ala Asp Lys 
225                 230                 235                 240 


Val Leu His Lys Leu Gln Asn Leu Gly Asn Arg Leu Asn Leu Gly Leu 
                245                 250                 255     


Phe Arg Pro Ala Ala Thr Ser Arg Val Leu Glu Trp Met Ile Arg His 
            260                 265                 270         


Gln Asp Phe Asp Gly Ala Trp Gly Gly Ile Gln Pro Pro Trp Ile Tyr 
        275                 280                 285             


Gly Leu Met Ala Leu Tyr Ala Glu Gly Tyr Pro Leu Asn His Pro Val 
    290                 295                 300                 


Leu Ala Lys Gly Leu Asp Ala Leu Asn Asp Pro Gly Trp Arg Val Asp 
305                 310                 315                 320 


Val Gly Asp Ala Thr Tyr Ile Gln Ala Thr Asn Ser Pro Val Trp Asp 
                325                 330                 335     


Thr Ile Leu Thr Leu Leu Ala Phe Asp Asp Ala Gly Val Leu Gly Asp 
            340                 345                 350         


Tyr Pro Glu Ala Val Asp Lys Ala Val Asp Trp Val Leu Gln Arg Gln 
        355                 360                 365             


Val Arg Val Pro Gly Asp Trp Ser Met Lys Leu Pro His Val Lys Pro 
    370                 375                 380                 


Gly Gly Trp Ala Phe Glu Tyr Ala Asn Asn Tyr Tyr Pro Asp Thr Asp 
385                 390                 395                 400 


Asp Thr Ala Val Ala Leu Ile Ala Leu Ala Pro Leu Arg His Asp Pro 
                405                 410                 415     


Lys Trp Lys Ala Lys Gly Ile Asp Glu Ala Ile Gln Leu Gly Val Asp 
            420                 425                 430         


Trp Leu Ile Gly Met Gln Ser Gln Gly Gly Gly Trp Gly Ala Phe Asp 
        435                 440                 445             


Lys Asp Asn Asn Gln Lys Ile Leu Thr Lys Ile Pro Phe Cys Asp Tyr 
    450                 455                 460                 


Gly Glu Ala Leu Asp Pro Pro Ser Val Asp Val Thr Ala His Ile Ile 
465                 470                 475                 480 


Glu Ala Phe Gly Lys Leu Gly Ile Ser Arg Asn His Pro Ser Met Val 
                485                 490                 495     


Gln Ala Leu Asp Tyr Ile Arg Arg Glu Gln Glu Pro Ser Gly Pro Trp 
            500                 505                 510         


Phe Gly Arg Trp Gly Val Asn Tyr Val Tyr Gly Thr Gly Ala Val Leu 
        515                 520                 525             


Pro Ala Leu Ala Ala Ile Gly Glu Asp Met Thr Gln Pro Tyr Ile Gly 
    530                 535                 540                 


Arg Ala Cys Asp Trp Leu Val Ala His Gln Gln Ala Asp Gly Gly Trp 
545                 550                 555                 560 


Gly Glu Ser Cys Ala Ser Tyr Met Asp Val Ser Ala Val Gly Arg Gly 
                565                 570                 575     


Thr Thr Thr Ala Ser Gln Thr Ala Trp Ala Leu Met Ala Leu Leu Ala 
            580                 585                 590         


Ala Asn Arg Pro Gln Asp Lys Asp Ala Ile Glu Arg Gly Cys Met Trp 
        595                 600                 605             


Leu Val Glu Arg Gln Ser Ala Gly Thr Trp Asp Glu Pro Glu Phe Thr 
    610                 615                 620                 


Gly Thr Gly Phe Pro Gly Tyr Gly Val Gly Gln Thr Ile Lys Leu Asn 
625                 630                 635                 640 


Asp Pro Ala Leu Ser Gln Arg Leu Met Gln Gly Pro Glu Leu Ser Arg 
                645                 650                 655     


Ala Phe Met Leu Arg Tyr Gly Met Tyr Arg His Tyr Phe Pro Leu Met 
            660                 665                 670         


Ala Leu Gly Arg Ala Leu Arg Pro Gln Ser His Ser 
        675                 680                 


<210>  18
<211>  642
<212>  PRT
<213>  Thermosynechococcus elongatus wt SHC enzyme

<400>  18

Met Pro Thr Ser Leu Ala Thr Ala Ile Asp Pro Lys Gln Leu Gln Gln 
1               5                   10                  15      


Ala Ile Arg Ala Ser Gln Asp Phe Leu Phe Ser Gln Gln Tyr Ala Glu 
            20                  25                  30          


Gly Tyr Trp Trp Ala Glu Leu Glu Ser Asn Val Thr Met Thr Ala Glu 
        35                  40                  45              


Val Ile Leu Leu His Lys Ile Trp Gly Thr Glu Gln Arg Leu Pro Leu 
    50                  55                  60                  


Ala Lys Ala Glu Gln Tyr Leu Arg Asn His Gln Arg Asp His Gly Gly 
65                  70                  75                  80  


Trp Glu Leu Phe Tyr Gly Asp Gly Gly Asp Leu Ser Thr Ser Val Glu 
                85                  90                  95      


Ala Tyr Met Gly Leu Arg Leu Leu Gly Val Pro Glu Thr Asp Pro Ala 
            100                 105                 110         


Leu Val Lys Ala Arg Gln Phe Ile Leu Ala Arg Gly Gly Ile Ser Lys 
        115                 120                 125             


Thr Arg Ile Phe Thr Lys Leu His Leu Ala Leu Ile Gly Cys Tyr Asp 
    130                 135                 140                 


Trp Arg Gly Ile Pro Ser Leu Pro Pro Trp Ile Met Leu Leu Pro Glu 
145                 150                 155                 160 


Gly Ser Pro Phe Thr Ile Tyr Glu Met Ser Ser Trp Ala Arg Ser Ser 
                165                 170                 175     


Thr Val Pro Leu Leu Ile Val Met Asp Arg Lys Pro Val Tyr Gly Met 
            180                 185                 190         


Asp Pro Pro Ile Thr Leu Asp Glu Leu Tyr Ser Glu Gly Arg Ala Asn 
        195                 200                 205             


Val Val Trp Glu Leu Pro Arg Gln Gly Asp Trp Arg Asp Val Phe Ile 
    210                 215                 220                 


Gly Leu Asp Arg Val Phe Lys Leu Phe Glu Thr Leu Asn Ile His Pro 
225                 230                 235                 240 


Leu Arg Glu Gln Gly Leu Lys Ala Ala Glu Glu Trp Val Leu Glu Arg 
                245                 250                 255     


Gln Glu Ala Ser Gly Asp Trp Gly Gly Ile Ile Pro Ala Met Leu Asn 
            260                 265                 270         


Ser Leu Leu Ala Leu Arg Ala Leu Asp Tyr Ala Val Asp Asp Pro Ile 
        275                 280                 285             


Val Gln Arg Gly Met Ala Ala Val Asp Arg Phe Ala Ile Glu Thr Glu 
    290                 295                 300                 


Thr Glu Tyr Arg Val Gln Pro Cys Val Ser Pro Val Trp Asp Thr Ala 
305                 310                 315                 320 


Leu Val Met Arg Ala Met Val Asp Ser Gly Val Ala Pro Asp His Pro 
                325                 330                 335     


Ala Leu Val Lys Ala Gly Glu Trp Leu Leu Ser Lys Gln Ile Leu Asp 
            340                 345                 350         


Tyr Gly Asp Trp His Ile Lys Asn Lys Lys Gly Arg Pro Gly Gly Trp 
        355                 360                 365             


Ala Phe Glu Phe Glu Asn Arg Phe Tyr Pro Asp Val Asp Asp Thr Ala 
    370                 375                 380                 


Val Val Val Met Ala Leu His Ala Val Thr Leu Pro Asn Glu Asn Leu 
385                 390                 395                 400 


Lys Arg Arg Ala Ile Glu Arg Ala Val Ala Trp Ile Ala Ser Met Gln 
                405                 410                 415     


Cys Arg Pro Gly Gly Trp Ala Ala Phe Asp Val Asp Asn Asp Gln Asp 
            420                 425                 430         


Trp Leu Asn Gly Ile Pro Tyr Gly Asp Leu Lys Ala Met Ile Asp Pro 
        435                 440                 445             


Asn Thr Ala Asp Val Thr Ala Arg Val Leu Glu Met Val Gly Arg Cys 
    450                 455                 460                 


Gln Leu Ala Phe Asp Arg Val Ala Leu Asp Arg Ala Leu Ala Tyr Leu 
465                 470                 475                 480 


Arg Asn Glu Gln Glu Pro Glu Gly Cys Trp Phe Gly Arg Trp Gly Val 
                485                 490                 495     


Asn Tyr Leu Tyr Gly Thr Ser Gly Val Leu Thr Ala Leu Ser Leu Val 
            500                 505                 510         


Ala Pro Arg Tyr Asp Arg Trp Arg Ile Arg Arg Ala Ala Glu Trp Leu 
        515                 520                 525             


Met Gln Cys Gln Asn Ala Asp Gly Gly Trp Gly Glu Thr Cys Trp Ser 
    530                 535                 540                 


Tyr His Asp Pro Ser Leu Lys Gly Lys Gly Asp Ser Thr Ala Ser Gln 
545                 550                 555                 560 


Thr Ala Trp Ala Ile Ile Gly Leu Leu Ala Ala Gly Asp Ala Thr Gly 
                565                 570                 575     


Asp Tyr Ala Thr Glu Ala Ile Glu Arg Gly Ile Ala Tyr Leu Leu Glu 
            580                 585                 590         


Thr Gln Arg Pro Asp Gly Thr Trp His Glu Asp Tyr Phe Thr Gly Thr 
        595                 600                 605             


Gly Phe Pro Cys His Phe Tyr Leu Lys Tyr His Tyr Tyr Gln Gln His 
    610                 615                 620                 


Phe Pro Leu Thr Ala Leu Gly Arg Tyr Ala Arg Trp Arg Asn Leu Leu 
625                 630                 635                 640 


Ala Thr 
        


<210>  19
<211>  720
<212>  PRT
<213>  Acetobacter pasteurianus wt SHC enzyme

<400>  19

Met Asn Met Ala Ser Arg Phe Ser Leu Lys Lys Ile Leu Arg Ser Gly 
1               5                   10                  15      


Ser Asp Thr Gln Gly Thr Asn Val Asn Thr Leu Ile Gln Ser Gly Thr 
            20                  25                  30          


Ser Asp Ile Val Arg Gln Lys Pro Ala Pro Gln Glu Pro Ala Asp Leu 
        35                  40                  45              


Ser Ala Leu Lys Ala Met Gly Asn Ser Leu Thr His Thr Leu Ser Ser 
    50                  55                  60                  


Ala Cys Glu Trp Leu Met Lys Gln Gln Lys Pro Asp Gly His Trp Val 
65                  70                  75                  80  


Gly Ser Val Gly Ser Asn Ala Ser Met Glu Ala Glu Trp Cys Leu Ala 
                85                  90                  95      


Leu Trp Phe Leu Gly Leu Glu Asp His Pro Leu Arg Pro Arg Leu Gly 
            100                 105                 110         


Lys Ala Leu Leu Glu Met Gln Arg Pro Asp Gly Ser Trp Gly Thr Tyr 
        115                 120                 125             


Tyr Gly Ala Gly Ser Gly Asp Ile Asn Ala Thr Val Glu Ser Tyr Ala 
    130                 135                 140                 


Ala Leu Arg Ser Leu Gly Tyr Ala Glu Asp Asp Pro Ala Val Ser Lys 
145                 150                 155                 160 


Ala Ala Ala Trp Ile Ile Ser Lys Gly Gly Leu Lys Asn Val Arg Val 
                165                 170                 175     


Phe Thr Arg Tyr Trp Leu Ala Leu Ile Gly Glu Trp Pro Trp Glu Lys 
            180                 185                 190         


Thr Pro Asn Leu Pro Pro Glu Ile Ile Trp Phe Pro Asp Asn Phe Val 
        195                 200                 205             


Phe Ser Ile Tyr Asn Phe Ala Gln Trp Ala Arg Ala Thr Met Met Pro 
    210                 215                 220                 


Leu Ala Ile Leu Ser Ala Arg Arg Pro Ser Arg Pro Leu Arg Pro Gln 
225                 230                 235                 240 


Asp Arg Leu Asp Ala Leu Phe Pro Gly Gly Arg Ala Asn Phe Asp Tyr 
                245                 250                 255     


Glu Leu Pro Thr Lys Glu Gly Arg Asp Val Ile Ala Asp Phe Phe Arg 
            260                 265                 270         


Leu Ala Asp Lys Gly Leu His Trp Leu Gln Ser Ser Phe Leu Lys Arg 
        275                 280                 285             


Ala Pro Ser Arg Glu Ala Ala Ile Lys Tyr Val Leu Glu Trp Ile Ile 
    290                 295                 300                 


Trp His Gln Asp Ala Asp Gly Gly Trp Gly Gly Ile Gln Pro Pro Trp 
305                 310                 315                 320 


Val Tyr Gly Leu Met Ala Leu His Gly Glu Gly Tyr Gln Phe His His 
                325                 330                 335     


Pro Val Met Ala Lys Ala Leu Asp Ala Leu Asn Asp Pro Gly Trp Arg 
            340                 345                 350         


His Asp Lys Gly Asp Ala Ser Trp Ile Gln Ala Thr Asn Ser Pro Val 
        355                 360                 365             


Trp Asp Thr Met Leu Ser Leu Met Ala Leu His Asp Ala Asn Ala Glu 
    370                 375                 380                 


Glu Arg Phe Thr Pro Glu Met Asp Lys Ala Leu Asp Trp Leu Leu Ser 
385                 390                 395                 400 


Arg Gln Val Arg Val Lys Gly Asp Trp Ser Val Lys Leu Pro Asn Thr 
                405                 410                 415     


Glu Pro Gly Gly Trp Ala Phe Glu Tyr Ala Asn Asp Arg Tyr Pro Asp 
            420                 425                 430         


Thr Asp Asp Thr Ala Val Ala Leu Ile Ala Ile Ala Ser Cys Arg Asn 
        435                 440                 445             


Arg Pro Glu Trp Gln Ala Lys Gly Val Glu Glu Ala Ile Gly Arg Gly 
    450                 455                 460                 


Val Arg Trp Leu Val Ala Met Gln Ser Ser Cys Gly Gly Trp Gly Ala 
465                 470                 475                 480 


Phe Asp Lys Asp Asn Asn Lys Ser Ile Leu Ala Lys Ile Pro Phe Cys 
                485                 490                 495     


Asp Phe Gly Glu Ala Leu Asp Pro Pro Ser Val Asp Val Thr Ala His 
            500                 505                 510         


Val Leu Glu Ala Phe Gly Leu Leu Gly Leu Pro Arg Asp Leu Pro Cys 
        515                 520                 525             


Ile Gln Arg Gly Leu Ala Tyr Ile Arg Lys Glu Gln Asp Pro Thr Gly 
    530                 535                 540                 


Pro Trp Phe Gly Arg Trp Gly Val Asn Tyr Leu Tyr Gly Thr Gly Ala 
545                 550                 555                 560 


Val Leu Pro Ala Leu Ala Ala Leu Gly Glu Asp Met Thr Gln Pro Tyr 
                565                 570                 575     


Ile Ser Lys Ala Cys Asp Trp Leu Ile Asn Cys Gln Gln Glu Asn Gly 
            580                 585                 590         


Gly Trp Gly Glu Ser Cys Ala Ser Tyr Met Glu Val Ser Ser Ile Gly 
        595                 600                 605             


His Gly Ala Thr Thr Pro Ser Gln Thr Ala Trp Ala Leu Met Gly Leu 
    610                 615                 620                 


Ile Ala Ala Asn Arg Pro Gln Asp Tyr Glu Ala Ile Ala Lys Gly Cys 
625                 630                 635                 640 


Arg Tyr Leu Ile Asp Leu Gln Glu Glu Asp Gly Ser Trp Asn Glu Glu 
                645                 650                 655     


Glu Phe Thr Gly Thr Gly Phe Pro Gly Tyr Gly Val Gly Gln Thr Ile 
            660                 665                 670         


Lys Leu Asp Asp Pro Ala Ile Ser Lys Arg Leu Met Gln Gly Ala Glu 
        675                 680                 685             


Leu Ser Arg Ala Phe Met Leu Arg Tyr Asp Leu Tyr Arg Gln Leu Phe 
    690                 695                 700                 


Pro Ile Ile Ala Leu Ser Arg Ala Ser Arg Leu Ile Lys Leu Gly Asn 
705                 710                 715                 720 


<210>  20
<211>  685
<212>  PRT
<213>  Gluconobacter morbifer wt SHC enzyme

<400>  20

Met Ser Pro Ala Asp Ile Ser Thr Lys Ser Ser Ser Phe Gln Arg Leu 
1               5                   10                  15      


Asp Asn Met Leu Pro Glu Ala Val Ser Ser Ala Cys Asp Trp Leu Ile 
            20                  25                  30          


Asp Gln Gln Lys Pro Asp Gly His Trp Val Gly Pro Val Glu Ser Asn 
        35                  40                  45              


Ala Cys Met Glu Ala Gln Trp Cys Leu Ala Leu Trp Phe Leu Gly Gln 
    50                  55                  60                  


Glu Asp His Pro Leu Arg Pro Arg Leu Ala Gln Ala Leu Leu Glu Met 
65                  70                  75                  80  


Gln Arg Glu Asp Gly Ser Trp Gly Ile Tyr Val Gly Ala Asp His Gly 
                85                  90                  95      


Asp Ile Asn Thr Thr Val Glu Ala Tyr Ala Ala Leu Arg Ser Met Gly 
            100                 105                 110         


Tyr Ala Ala Asp Met Pro Ile Met Ala Lys Ser Ala Ala Trp Ile Gln 
        115                 120                 125             


Gln Lys Gly Gly Leu Arg Asn Val Arg Val Phe Thr Arg Tyr Trp Leu 
    130                 135                 140                 


Ala Leu Ile Gly Glu Trp Pro Trp Asp Lys Thr Pro Asn Leu Pro Pro 
145                 150                 155                 160 


Glu Ile Ile Trp Leu Pro Asp Asn Phe Ile Phe Ser Ile Tyr Asn Phe 
                165                 170                 175     


Ala Gln Trp Ala Arg Ala Thr Met Met Pro Leu Thr Ile Leu Ser Ala 
            180                 185                 190         


Arg Arg Pro Ser Arg Pro Leu Leu Pro Glu Asn Arg Leu Asp Gly Leu 
        195                 200                 205             


Phe Pro Glu Gly Arg Glu Asn Phe Asp Tyr Glu Leu Pro Val Lys Gly 
    210                 215                 220                 


Glu Glu Asp Leu Trp Gly Arg Phe Phe Arg Ala Ala Asp Lys Gly Leu 
225                 230                 235                 240 


His Ser Leu Gln Ser Phe Pro Val Arg Arg Phe Val Pro Arg Glu Ala 
                245                 250                 255     


Ala Ile Arg His Val Ile Glu Trp Ile Ile Arg His Gln Asp Ala Asp 
            260                 265                 270         


Gly Gly Trp Gly Gly Ile Gln Pro Pro Trp Ile Tyr Gly Leu Met Ala 
        275                 280                 285             


Leu Ser Val Glu Gly Tyr Pro Leu His His Pro Val Leu Ala Lys Ala 
    290                 295                 300                 


Met Asp Ala Leu Asn Asp Pro Gly Trp Arg Arg Asp Lys Gly Asp Ala 
305                 310                 315                 320 


Ser Trp Ile Gln Ala Thr Asn Ser Pro Val Trp Asp Thr Met Leu Ala 
                325                 330                 335     


Val Leu Ala Leu His Asp Ala Gly Ala Glu Asp Arg Tyr Ser Pro Gln 
            340                 345                 350         


Met Asp Lys Ala Ile Gly Trp Leu Leu Asp Arg Gln Val Arg Val Lys 
        355                 360                 365             


Gly Asp Trp Ser Ile Lys Leu Pro Asp Thr Glu Pro Gly Gly Trp Ala 
    370                 375                 380                 


Phe Glu Tyr Ala Asn Asp Lys Tyr Pro Asp Thr Asp Asp Thr Ala Val 
385                 390                 395                 400 


Ala Leu Ile Ala Leu Ala Gly Cys Arg His Arg Pro Glu Trp Arg Glu 
                405                 410                 415     


Arg Asp Ile Glu Gly Ala Ile Ser Arg Gly Val Asn Trp Leu Leu Ala 
            420                 425                 430         


Met Gln Ser Ser Ser Gly Gly Trp Gly Ala Phe Asp Lys Asp Asn Asn 
        435                 440                 445             


Arg Ser Ile Leu Thr Lys Ile Pro Phe Cys Asp Phe Gly Glu Ala Leu 
    450                 455                 460                 


Asp Pro Pro Ser Val Asp Val Thr Ala His Val Leu Glu Ala Phe Gly 
465                 470                 475                 480 


Leu Leu Gly Ile Ser Arg Asn His Pro Ser Val Gln Lys Ala Leu Ala 
                485                 490                 495     


Tyr Ile Arg Ser Glu Gln Glu Arg Asn Gly Ala Trp Phe Gly Arg Trp 
            500                 505                 510         


Gly Val Asn Tyr Val Tyr Gly Thr Gly Ala Val Leu Pro Ala Leu Ala 
        515                 520                 525             


Ala Ile Gly Glu Asp Met Thr Gln Pro Tyr Ile Val Arg Ala Cys Asp 
    530                 535                 540                 


Trp Leu Met Ser Val Gln Gln Glu Asn Gly Gly Trp Gly Glu Ser Cys 
545                 550                 555                 560 


Ala Ser Tyr Met Asp Ile Asn Ala Val Gly His Gly Val Ala Thr Ala 
                565                 570                 575     


Ser Gln Thr Ala Trp Ala Leu Ile Gly Leu Leu Ala Ala Lys Arg Pro 
            580                 585                 590         


Lys Asp Arg Glu Ala Ile Ala Arg Gly Cys Gln Phe Leu Ile Glu Arg 
        595                 600                 605             


Gln Glu Asp Gly Ser Trp Thr Glu Glu Glu Tyr Thr Gly Thr Gly Phe 
    610                 615                 620                 


Pro Gly Tyr Gly Val Gly Gln Ala Ile Lys Leu Asp Asp Pro Ser Leu 
625                 630                 635                 640 


Pro Asp Arg Leu Leu Gln Gly Ala Glu Leu Ser Arg Ala Phe Met Leu 
                645                 650                 655     


Arg Tyr Asp Leu Tyr Arg Gln Tyr Phe Pro Val Met Ala Leu Ser Arg 
            660                 665                 670         


Ala Arg Arg Met Met Lys Glu Asp Ala Ser Ala Ala Ala 
        675                 680                 685 


<210>  21
<211>  625
<212>  PRT
<213>  Bacillus megaterium wt SHC enzyme

<400>  21

Met Ile Ile Leu Leu Lys Glu Val Gln Leu Glu Ile Gln Arg Arg Ile 
1               5                   10                  15      


Ala Tyr Leu Arg Pro Thr Gln Lys Asn Asp Gly Ser Phe Arg Tyr Cys 
            20                  25                  30          


Phe Glu Thr Gly Val Met Pro Asp Ala Phe Leu Ile Met Leu Leu Arg 
        35                  40                  45              


Thr Phe Asp Leu Asp Lys Glu Val Leu Ile Lys Gln Leu Thr Glu Arg 
    50                  55                  60                  


Ile Val Ser Leu Gln Asn Glu Asp Gly Leu Trp Thr Leu Phe Asp Asp 
65                  70                  75                  80  


Glu Glu His Asn Leu Ser Ala Thr Ile Gln Ala Tyr Thr Ala Leu Leu 
                85                  90                  95      


Tyr Ser Gly Tyr Tyr Gln Lys Asn Asp Arg Ile Leu Arg Lys Ala Glu 
            100                 105                 110         


Arg Tyr Ile Ile Asp Ser Gly Gly Ile Ser Arg Ala His Phe Leu Thr 
        115                 120                 125             


Arg Trp Met Leu Ser Val Asn Gly Leu Tyr Glu Trp Pro Lys Leu Phe 
    130                 135                 140                 


Tyr Leu Pro Leu Ser Leu Leu Leu Val Pro Thr Tyr Val Pro Leu Asn 
145                 150                 155                 160 


Phe Tyr Glu Leu Ser Thr Tyr Ala Arg Ile His Phe Val Pro Met Met 
                165                 170                 175     


Val Ala Gly Asn Lys Lys Phe Ser Leu Thr Ser Arg His Thr Pro Ser 
            180                 185                 190         


Leu Ser His Leu Asp Val Arg Glu Gln Lys Gln Glu Ser Glu Glu Thr 
        195                 200                 205             


Thr Gln Glu Ser Arg Ala Ser Ile Phe Leu Val Asp His Leu Lys Gln 
    210                 215                 220                 


Leu Ala Ser Leu Pro Ser Tyr Ile His Lys Leu Gly Tyr Gln Ala Ala 
225                 230                 235                 240 


Glu Arg Tyr Met Leu Glu Arg Ile Glu Lys Asp Gly Thr Leu Tyr Ser 
                245                 250                 255     


Tyr Ala Thr Ser Thr Phe Phe Met Ile Tyr Gly Leu Leu Ala Leu Gly 
            260                 265                 270         


Tyr Lys Lys Asp Ser Phe Val Ile Gln Lys Ala Ile Asp Gly Ile Cys 
        275                 280                 285             


Ser Leu Leu Ser Thr Cys Ser Gly His Val His Val Glu Asn Ser Thr 
    290                 295                 300                 


Ser Thr Val Trp Asp Thr Ala Leu Leu Ser Tyr Ala Leu Gln Glu Ala 
305                 310                 315                 320 


Gly Val Pro Gln Gln Asp Pro Met Ile Lys Gly Thr Thr Arg Tyr Leu 
                325                 330                 335     


Lys Lys Arg Gln His Thr Lys Leu Gly Asp Trp Gln Phe His Asn Pro 
            340                 345                 350         


Asn Thr Ala Pro Gly Gly Trp Gly Phe Ser Asp Ile Asn Thr Asn Asn 
        355                 360                 365             


Pro Asp Leu Asp Asp Thr Ser Ala Ala Ile Arg Ala Leu Ser Arg Arg 
    370                 375                 380                 


Ala Gln Thr Asp Thr Asp Tyr Leu Glu Ser Trp Gln Arg Gly Ile Asn 
385                 390                 395                 400 


Trp Leu Leu Ser Met Gln Asn Lys Asp Gly Gly Phe Ala Ala Phe Glu 
                405                 410                 415     


Lys Asn Thr Asp Ser Ile Leu Phe Thr Tyr Leu Pro Leu Glu Asn Ala 
            420                 425                 430         


Lys Asp Ala Ala Thr Asp Pro Ala Thr Ala Asp Leu Thr Gly Arg Val 
        435                 440                 445             


Leu Glu Cys Leu Gly Asn Phe Ala Gly Met Asn Lys Ser His Pro Ser 
    450                 455                 460                 


Ile Lys Ala Ala Val Lys Trp Leu Phe Asp His Gln Leu Asp Asn Gly 
465                 470                 475                 480 


Ser Trp Tyr Gly Arg Trp Gly Val Cys Tyr Ile Tyr Gly Thr Trp Ala 
                485                 490                 495     


Ala Ile Thr Gly Leu Arg Ala Val Gly Val Ser Ala Ser Asp Pro Arg 
            500                 505                 510         


Ile Ile Lys Ala Ile Asn Trp Leu Lys Ser Ile Gln Gln Glu Asp Gly 
        515                 520                 525             


Gly Phe Gly Glu Ser Cys Tyr Ser Ala Ser Leu Lys Lys Tyr Val Pro 
    530                 535                 540                 


Leu Ser Phe Ser Thr Pro Ser Gln Thr Ala Trp Ala Leu Asp Ala Leu 
545                 550                 555                 560 


Met Thr Ile Cys Pro Leu Lys Asp Gln Ser Val Glu Lys Gly Ile Lys 
                565                 570                 575     


Phe Leu Leu Asn Pro Asn Leu Thr Glu Gln Gln Thr His Tyr Pro Thr 
            580                 585                 590         


Gly Ile Gly Leu Pro Gly Gln Phe Tyr Ile Gln Tyr His Ser Tyr Asn 
        595                 600                 605             


Asp Ile Phe Pro Leu Leu Ala Leu Ala His Tyr Ala Lys Lys His Ser 
    610                 615                 620                 


Ser 
625 


<210>  22
<211>  631
<212>  PRT
<213>  SHC enzyme variant #49

<400>  22

Met Ala Glu Gln Leu Val Glu Ala Pro Ala Tyr Ala Arg Thr Leu Asp 
1               5                   10                  15      


Arg Ala Val Glu Tyr Leu Leu Ser Cys Gln Lys Asp Glu Gly Tyr Trp 
            20                  25                  30          


Trp Gly Pro Leu Leu Ser Asn Val Thr Met Glu Ala Glu Tyr Val Leu 
        35                  40                  45              


Leu Cys His Ile Leu Asp Arg Val Asp Arg Asp Arg Met Glu Lys Ile 
    50                  55                  60                  


Arg Arg Tyr Leu Leu His Glu Gln Arg Glu Asp Gly Thr Trp Ala Leu 
65                  70                  75                  80  


Tyr Pro Gly Gly Pro Pro Asp Leu Asp Thr Thr Ile Glu Ala Tyr Val 
                85                  90                  95      


Ala Leu Lys Tyr Ile Gly Met Ser Arg Asp Glu Glu Pro Met Gln Lys 
            100                 105                 110         


Ala Leu Arg Phe Ile Gln Ser Gln Gly Gly Ile Glu Ser Ser Arg Val 
        115                 120                 125             


Phe Thr Arg Arg Trp Leu Ala Leu Val Gly Glu Tyr Pro Trp Glu Lys 
    130                 135                 140                 


Val Pro Met Val Pro Pro Glu Ile Met Phe Leu Gly Lys Arg Met Pro 
145                 150                 155                 160 


Leu Asn Ile Tyr Glu Phe Gly Ser Trp Ala Arg Ala Thr Val Val Ala 
                165                 170                 175     


Leu Ser Ile Val Met Ser Arg Gln Pro Val Phe Pro Leu Pro Glu Arg 
            180                 185                 190         


Ala Arg Val Pro Glu Leu Tyr Glu Thr Asp Val Pro Pro Arg Arg Arg 
        195                 200                 205             


Gly Ala Lys Gly Gly Gly Gly Trp Ile Phe Asp Ala Leu Asp Arg Val 
    210                 215                 220                 


Leu His Gly Tyr Gln Lys Leu Ser Val His Pro Phe Arg Arg Ala Ala 
225                 230                 235                 240 


Glu Ile Arg Ala Leu Asp Trp Leu Leu Glu Arg Gln Ala Gly Asp Gly 
                245                 250                 255     


Ser Trp Gly Gly Ile Gln Pro Pro Trp Phe Tyr Ala Leu Ile Ala Leu 
            260                 265                 270         


Lys Ile Leu Asp Met Thr Gln His Pro Ala Phe Ile Lys Gly Trp Glu 
        275                 280                 285             


Gly Leu Glu Leu Tyr Gly Val Glu Leu Asp Tyr Gly Gly Trp Met Phe 
    290                 295                 300                 


Gln Ala Ser Ile Ser Pro Val Trp Asp Thr Gly Leu Ala Val Leu Ala 
305                 310                 315                 320 


Leu Arg Ala Ala Gly Leu Pro Ala Asp His Asp Arg Leu Val Lys Ala 
                325                 330                 335     


Gly Glu Trp Leu Leu Asp Arg Gln Ile Thr Val Pro Gly Asp Trp Ala 
            340                 345                 350         


Val Lys Arg Pro Asn Leu Lys Pro Gly Gly Phe Ala Phe Gln Phe Asp 
        355                 360                 365             


Asn Val Tyr Tyr Pro Asp Val Asp Asp Thr Ala Val Val Val Trp Ala 
    370                 375                 380                 


Leu Asn Thr Leu Arg Leu Pro Asp Glu Arg Arg Arg Arg Asp Ala Met 
385                 390                 395                 400 


Thr Lys Gly Phe Arg Trp Ile Val Gly Met Gln Ser Ser Asn Gly Gly 
                405                 410                 415     


Trp Gly Ala Tyr Asp Val Asp Asn Thr Ser Asp Leu Pro Asn Leu Thr 
            420                 425                 430         


Pro Phe Cys Asp Phe Gly Glu Val Thr Asp Pro Pro Ser Glu Asp Val 
        435                 440                 445             


Thr Ala His Val Leu Glu Cys Phe Gly Ser Phe Gly Tyr Asp Asp Ala 
    450                 455                 460                 


Trp Lys Val Ile Arg Arg Ala Val Glu Tyr Leu Lys Arg Glu Gln Lys 
465                 470                 475                 480 


Pro Asp Gly Ser Trp Phe Gly Arg Trp Gly Val Asn Tyr Leu Tyr Gly 
                485                 490                 495     


Thr Gly Ala Val Val Ser Ala Leu Lys Ala Val Gly Ile Asp Thr Arg 
            500                 505                 510         


Glu Pro Tyr Ile Gln Lys Ala Leu Asp Trp Val Glu Gln His Gln Asn 
        515                 520                 525             


Pro Asp Gly Gly Trp Gly Glu Asp Cys Arg Ser Tyr Glu Asp Pro Ala 
    530                 535                 540                 


Tyr Ala Gly Lys Gly Ala Ser Thr Pro Ser Gln Thr Thr Trp Ala Leu 
545                 550                 555                 560 


Met Ala Leu Ile Ala Gly Gly Arg Ala Glu Ser Glu Ala Ala Arg Arg 
                565                 570                 575     


Gly Val Gln Tyr Leu Val Glu Thr Gln Arg Pro Asp Gly Gly Trp Asp 
            580                 585                 590         


Glu Pro Tyr Tyr Thr Gly Thr Gly Phe Pro Gly Asp Phe Tyr Leu Gly 
        595                 600                 605             


Tyr Thr Met Tyr Arg His Val Phe Pro Thr Leu Ala Leu Gly Arg Tyr 
    610                 615                 620                 


Lys Gln Ala Ile Glu Arg Arg 
625                 630     

