			SEQUENCE LISTING

<110> CODEXIS, INC.
 
<120> CARBOXYESTERASE POLYPEPTIDES FOR KINETIC RESOLUTION
 
<130> CX2-206WO1
 
<140> 
<141> 2021-04-06

<150> US 63/008,061
<151> 2020-04-10
 
<160> 536
 
<210> 1
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Wild Type Esterase from  Xanthomonas campestris
 
<400> 1
atgccaagtc ctgttactgg tcgctggatg atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatcaac aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 2
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Wild Type Esterase from  Xanthomonas campestris
 
<400> 2
Met Pro Ser Pro Val Thr Gly Arg Trp Met Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Asn Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 3
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 3
atgcctagac ctgtaacagg tcgttgtatg attttggatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatcaac aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 4
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 4
Met Pro Arg Pro Val Thr Gly Arg Cys Met Ile Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Asn Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 5
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 5
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca gactggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgtgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttccc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaagcat tgcaagcatt aggtttcacg 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgcactt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 6
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 6
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Asp Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Pro Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 7
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 7
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaagt tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcatgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccggg 480
caactgcaac cgcaagcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaagcccg cttcaccgcg gtt                              693

 
<210> 8
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 8
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Val Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg His Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Gly 
145                 150                 155                 160 
Gln Leu Gln Pro Gln Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 9
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 9
atgccgagcc ccgtgactgg tcgatgcatt gagttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtcagtcca caatggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttccc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaagcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgcactt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 10
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 10
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Glu Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Ser Pro Gln Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Pro Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 11
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 11
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca gactggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcatgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaaatg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaagcat tgcaagcatt aggtttcacg 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgcactt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 12
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 12
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Asp Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Met Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Met Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 13
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 13
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaagt ggaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcatgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttccc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacg 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatcatctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 14
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 14
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Val Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Met Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Pro Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp His Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 15
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 15
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca gactggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcatgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgtcggctcg cttcaccgcg gtt                              693

 
<210> 16
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 16
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Asp Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Met Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Ser Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 17
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 17
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca gactggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcatgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatcatctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 18
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 18
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Asp Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Met Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp His Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 19
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 19
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca gactggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcctgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc tcatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgcactt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 20
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 20
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Asp Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Leu Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala His Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 21
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 21
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaagt ggaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtcagtcca gactggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttccc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatcatctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 22
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 22
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Val Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Ser Pro Asp Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Pro Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp His Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 23
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 23
atgccgagcc ccgtgactgg tcgatgcatt gagttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtcagtcca caatggccag cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttccc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc tcatgaaatg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttccat 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 24
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 24
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Glu Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Ser Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Pro Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala His Glu Met Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe His Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 25
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 25
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaatg cgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca gactggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgtgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttccc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc tcatgaaatg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttccat 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgcactt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 26
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 26
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Cys Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Asp Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Pro Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala His Glu Met Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe His Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 27
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 27
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtcagtcca gactggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taacgaaatg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaagctcg cttcaccgcg gtt                              693

 
<210> 28
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 28
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Ser Pro Asp Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Met Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 29
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 29
atgccgagcc ccgtgactgg tcgatgcatt atgccggacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca gactggccag cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcctgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttccat 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 30
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 30
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Pro Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Asp Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Leu Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe His Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 31
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 31
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaaca cgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaaatg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 32
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 32
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
His Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Met Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 33
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 33
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg cgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca gactggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcctgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaaatg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacg 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgcactt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 34
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 34
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Asp Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Leu Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Met Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 35
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 35
atgccgagcc ccgtgactgg tcgatgcatt gagttagacg tgattgaaca cgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcatgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttccc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacg 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatcatctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 36
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 36
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Glu Leu Asp Val Ile Glu 
1               5                   10                  15      
His Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Met Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Pro Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp His Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 37
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 37
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaagt ggaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcctgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttccc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc tcatgaaatg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttccat 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 38
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 38
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Val Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Leu Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Pro Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala His Glu Met Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe His Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 39
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 39
atgccgagcc ccgtgactgg tcgatgcatt gagttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtcagtcca gactggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgtgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttccc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc tcatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaagcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 40
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 40
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Glu Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Ser Pro Asp Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Pro Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala His Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 41
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 41
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaagt tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgtagca 300
caagttgaaa tgctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccgag 480
caactgcaac cgcaagcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 42
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 42
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Val Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Met Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Glu 
145                 150                 155                 160 
Gln Leu Gln Pro Gln Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 43
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 43
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtcagtcca cagtggccag cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcctgc gcatgcgtgg ttggtacgac 240
atcgggggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc tcacgaaatg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacg 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgtcggctcg cttcaccgcg gtt                              693

 
<210> 44
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 44
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Ser Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Leu Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gly Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala His Glu Met Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Ser Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 45
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 45
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca gactggccag cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacg 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatcatctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 46
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 46
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Asp Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp His Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 47
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 47
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacg 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgcactt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 48
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 48
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 49
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 49
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtcagtcca gactggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcatgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 50
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 50
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Ser Pro Asp Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Met Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 51
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 51
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttccc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc tcatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaagcat tgcaagcatt aggtttccat 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 52
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 52
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Pro Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala His Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe His Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 53
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 53
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaagt ggaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtcagtcca caatggccag cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgtgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaagcat tgcaagcatt aggtttcacg 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgcactt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 54
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 54
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Val Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Ser Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 55
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 55
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaaca cgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgtgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttccc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc tcatgaaatg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacg 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgcactt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 56
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 56
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
His Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Pro Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala His Glu Met Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 57
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 57
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atcgggggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 58
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 58
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gly Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 59
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 59
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca gactggccag cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccagggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taacgaaatg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttccat 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaagctcg cttcaccgcg gtt                              693

 
<210> 60
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 60
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Asp Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Met Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe His Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 61
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 61
atgccgagcc ccgtgactgg tcgagcgatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 62
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 62
Met Pro Ser Pro Val Thr Gly Arg Ala Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 63
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 63
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaatg cgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttccc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcaatggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatcatctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 64
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 64
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Cys Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Pro Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Met 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp His Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 65
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 65
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttccc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgcactt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 66
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 66
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Pro Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 67
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 67
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg cgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcatgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttccc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttccat 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgcactt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 68
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 68
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Met Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Pro Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe His Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 69
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 69
atgccgagcc ccgtgactgg tcgatgcatt atgctggacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca gactggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacg 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaagctcg cttcaccgcg gtt                              693

 
<210> 70
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 70
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Asp Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 71
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 71
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttccc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc tcatgaaatg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 72
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 72
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Pro Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala His Glu Met Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 73
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 73
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtcagtcca caatggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttccc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaaatg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaagcat tgcaagcatt aggtttcacg 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgcactt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 74
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 74
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Ser Pro Gln Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Pro Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Met Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 75
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 75
atgccgagcc ccgtgactgg tcgatgcatt gagttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcatgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttccc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaaatg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaagcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 76
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 76
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Glu Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Met Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Pro Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Met Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 77
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 77
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaagt ggaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca gactggccag cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcatgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttccc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc tcatgaaatg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacg 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatcatctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 78
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 78
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Val Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Asp Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Met Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Pro Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala His Glu Met Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp His Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 79
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 79
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtcagtcca gactggccag cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccagggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taacgaacaa gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgtcggctcg cttcaccgcg gtt                              693

 
<210> 80
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 80
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Ser Pro Asp Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Ser Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 81
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 81
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaaca tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 82
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 82
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
His Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 83
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 83
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca gactggccag cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgtgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc tcatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacg 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 84
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 84
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Asp Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala His Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 85
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 85
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaaca cgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtcagtcca gactggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcatgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc tcatgaaatg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcaatggcat tgcaagcatt aggtttccat 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatcatctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 86
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 86
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
His Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Ser Pro Asp Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Met Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala His Glu Met Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Met 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe His Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp His Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 87
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 87
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtcagtcca gactggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcaatggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 88
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 88
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Ser Pro Asp Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Met 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 89
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 89
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtcagtcca cagtggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc tcacgaacaa gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaagcat tgcaagcatt aggtttcacg 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgtcggctcg cttcaccgcg gtt                              693

 
<210> 90
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 90
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Ser Pro Gln Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala His Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Ser Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 91
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 91
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcatgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttccc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcaatggcat tgcaagcatt aggtttcacg 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatcatctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 92
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 92
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Met Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Pro Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Met 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp His Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 93
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 93
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcatgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc tcatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcaatggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 94
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 94
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Met Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala His Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Met 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 95
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 95
atgccgagcc ccgtgactgg tcgacgcatt gagccggacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca gactggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcctgc gcatgcgtgg ttggtacgac 240
atccagggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taacgaaatg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaagcat tgcaagcatt aggtttcacg 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaagctcg cttcaccgcg gtt                              693

 
<210> 96
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 96
Met Pro Ser Pro Val Thr Gly Arg Arg Ile Glu Pro Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Asp Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Leu Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Met Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 97
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 97
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaagt ggaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcctgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc tcatgaaatg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaagcat tgcaagcatt aggtttcacg 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 98
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 98
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Val Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Leu Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala His Glu Met Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 99
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 99
atgccgagcc ccgtgactgg tcgatgcatt gggttagacg tgattgaagt ggaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca gactggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgtgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttccat 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgcactt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 100
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 100
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Gly Leu Asp Val Ile Glu 
1               5                   10                  15      
Val Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Asp Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe His Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 101
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 101
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtcagtcca gactggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgtgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 102
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 102
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Ser Pro Asp Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 103
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 103
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaagt ggaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaaatg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttccat 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgcactt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 104
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 104
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Val Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Met Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe His Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 105
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 105
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca gactggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgtgc gcatgcgtgg ttggtacgac 240
atccagggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttccat 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaagctcg cttcaccgcg gtt                              693

 
<210> 106
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 106
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Asp Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe His Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 107
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 107
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg cgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtcagtcca caatggccag cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcctgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttccc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc tcatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaagcat tgcaagcatt aggtttcacg 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgcactt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 108
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 108
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Ser Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Leu Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Pro Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala His Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 109
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 109
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtcagtcca caatggccag cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgtgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttccc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgcactt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 110
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 110
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Ser Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Pro Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 111
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 111
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcctgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc tcatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacg 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 112
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 112
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Leu Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala His Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 113
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 113
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtcagtcca caatggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcctgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttccc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaaatg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 114
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 114
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Ser Pro Gln Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Leu Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Pro Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Met Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 115
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 115
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcatgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc tcatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcaatggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatcatctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 116
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 116
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Met Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala His Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Met 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp His Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 117
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 117
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcctgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc tcatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaagcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgcactt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 118
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 118
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Leu Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala His Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 119
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 119
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttccc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc tcatgaaatg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcaatggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgcactt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 120
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 120
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Pro Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala His Glu Met Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Met 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 121
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 121
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcatgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc tcatgaaatg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaagcat tgcaagcatt aggtttccat 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatcatctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 122
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 122
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Met Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala His Glu Met Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe His Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp His Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 123
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 123
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca cagtggccag cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcatgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc tcacgaacaa gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaagcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaagctcg cttcaccgcg gtt                              693

 
<210> 124
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 124
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Met Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala His Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 125
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 125
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcatgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaagcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 126
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 126
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Met Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 127
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 127
atgccgagcc ccgtgactgg tcgatgcatt gagttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcatgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacg 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgcactt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 128
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 128
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Glu Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Met Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 129
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 129
atgccgagcc ccgtgactgg tcgatgcatt gagttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcctgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc tcatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgcactt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 130
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 130
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Glu Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Leu Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala His Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 131
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 131
atgccgagcc ccgtgactgg tcgatgcatt gagttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcctgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaagcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgcactt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 132
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 132
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Glu Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Leu Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 133
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 133
atgccgagcc ccgtgactgg tcgatgcatt atcttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcttgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 134
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 134
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Ile Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Leu Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 135
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 135
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaatg cgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcctgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc tcatgaaatg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttccat 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 136
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 136
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Cys Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Leu Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala His Glu Met Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe His Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 137
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 137
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtcagtcca gactggccag cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaaatg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacg 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 138
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 138
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Ser Pro Asp Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Met Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 139
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 139
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaatg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 140
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 140
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Cys Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 141
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 141
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcctgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc tcatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcaatggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgcactt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 142
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 142
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Leu Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala His Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Met 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 143
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 143
atgccgagcc ccgtgactgg tcgatgcatt atgccggacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtcagtcca gactggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc tcacgaacaa gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgtcggctcg cttcaccgcg gtt                              693

 
<210> 144
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 144
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Pro Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Ser Pro Asp Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala His Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Ser Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 145
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 145
atgccgagcc ccgtgactgg tcgatgcatt atcttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatcgtg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 146
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 146
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Ile Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Val Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 147
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 147
atgccgagcc ccgtgactgg tcgatgcatt gagttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtcagtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgtgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttccc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacg 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 148
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 148
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Glu Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Ser Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Pro Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 149
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 149
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcctgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttccc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc tcatgaaatg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaagcat tgcaagcatt aggtttcacg 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgcactt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 150
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 150
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Leu Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Pro Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala His Glu Met Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 151
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 151
atgccgagcc ccgtgactgg tcgatgcatt gggttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca gactggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcctgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc tcatgaaatg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacg 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgcactt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 152
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 152
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Gly Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Asp Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Leu Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala His Glu Met Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 153
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 153
atgccgagcc ccgtgactgg tcgatgcatt atcttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcatgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 154
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 154
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Ile Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Met Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 155
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 155
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaagt ggaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttccc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgcactt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 156
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 156
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Val Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Pro Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 157
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 157
atgccgagcc ccgtgactgg tcgatgcatt gagttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcatgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcaatggcat tgcaagcatt aggtttccat 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 158
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 158
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Glu Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Met Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Met 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe His Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 159
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 159
atgccgagcc ccgtgactgg tcgaactatt aggccggacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca gactggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcctgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc tcacgaacaa gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaagcat tgcaagcatt aggtttcacg 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgtcggctcg cttcaccgcg gtt                              693

 
<210> 160
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 160
Met Pro Ser Pro Val Thr Gly Arg Thr Ile Arg Pro Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Asp Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Leu Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala His Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Ser Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 161
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 161
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatcaac aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggaggaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 162
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 162
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Asn Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Glu Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 163
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 163
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaacg ggaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 164
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 164
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 165
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 165
atgccgcgcc ccgtgactgg tcgctgtatt acgttagatg tgattgaacg tgaaaccggg 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgatttt taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcacggcg gtt                              693

 
<210> 166
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 166
Met Pro Arg Pro Val Thr Gly Arg Cys Ile Thr Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Phe Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 167
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 167
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtcagtcca gactggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgtgc gcatgcgtgg ttggtacgac 240
atcgggggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc tcacgaaatg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacg 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgtcggctcg cttcaccgcg gtt                              693

 
<210> 168
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 168
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Ser Pro Asp Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gly Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala His Glu Met Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Ser Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 169
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 169
atgccgagcc ccgtgactgg tcgatgcatt gggttagacg tgattgaatg cgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtcagtcca caatggccag cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacg 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatcatctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 170
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 170
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Gly Leu Asp Val Ile Glu 
1               5                   10                  15      
Cys Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Ser Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp His Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 171
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 171
atgccgagcc ccgtgactgg tcgatgcatt atcttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtcaggatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 172
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 172
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Ile Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Gln Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 173
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 173
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaagt ggaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 174
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 174
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Val Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 175
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 175
atgccgagcc ctgtgccggg tcgctgcatt acgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctaacgg tagtgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgcccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgcagg agaacaaagt gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcatggcg gtt                              693

 
<210> 176
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 176
Met Pro Ser Pro Val Pro Gly Arg Cys Ile Thr Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asn Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Met Ala Val                                     
225                 230     

 
<210> 177
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 177
atgccgagcc ccgtgactgg tcgatgcatt atgctggacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcctgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcaatggcat tgcaagcatt aggtttcacg 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaagctcg cttcaccgcg gtt                              693

 
<210> 178
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 178
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Leu Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Met 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 179
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 179
atgccgagcc ccgtgactgg tcgatgtatt attttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 180
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 180
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Ile Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 181
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 181
atgccgagcc ccgtgactgg tcgctgtatt atgttagaag tgattgaacg tgaaaccggg 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcacggcg gtt                              693

 
<210> 182
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 182
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Glu Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 183
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 183
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtcagtcca gactggccag cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc tcatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatcatctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 184
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 184
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Ser Pro Asp Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala His Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp His Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 185
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 185
atgccgagcc ccgtgactgg tcgatgcatt gagttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttccc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaaatg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatcatctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 186
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 186
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Glu Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Pro Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Met Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp His Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 187
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 187
atgccgagcc ccgtgactgg tcgatgcatt gagttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgtgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttccc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcaatggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 188
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 188
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Glu Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Pro Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Met 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 189
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 189
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaagt ggaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc tcatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 190
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 190
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Val Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala His Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 191
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 191
atgccgagcc ccgtgactgg tcgatgcatt gagttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtcagtcca gactggccag cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc tcatgaaatg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 192
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 192
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Glu Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Ser Pro Asp Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala His Glu Met Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 193
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 193
atgccgagcc ccgtgactgg tcgatgcatt gggttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca gactggccag cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaaatg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcaatggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatcatctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 194
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 194
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Gly Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Asp Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Met Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Met 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp His Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 195
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 195
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatcaac aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgccaggg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 196
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 196
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Asn Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Gln Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 197
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 197
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaaatg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatcatctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 198
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 198
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Met Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp His Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 199
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 199
atgccgagcc gtgtgacggg tggctgcatt acgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgacgg tagtgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgcccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgcagg agaacaaagt gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcatggcg gtt                              693

 
<210> 200
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 200
Met Pro Ser Arg Val Thr Gly Gly Cys Ile Thr Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Met Ala Val                                     
225                 230     

 
<210> 201
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 201
atgccgagcc gtgtgacggg tggctgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctaacgg ttatgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgcccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgcagg agaacaaagt gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcatggcg gtt                              693

 
<210> 202
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 202
Met Pro Ser Arg Val Thr Gly Gly Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asn Gly Tyr Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Met Ala Val                                     
225                 230     

 
<210> 203
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 203
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgtgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttccc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc tcatgaaatg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcaatggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatcatctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 204
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 204
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Pro Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala His Glu Met Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Met 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp His Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 205
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 205
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaagc tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 206
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 206
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Ala Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 207
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 207
atgccgagcc ccgtgactgg tcgatgcatt gagttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtcagtcca gactggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttccc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc tcatgaaatg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaagcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatcatctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 208
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 208
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Glu Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Ser Pro Asp Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Pro Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala His Glu Met Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp His Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 209
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 209
atgccgagcc ccgtgactgg tcgatgcatt atcttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggccggc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 210
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 210
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Ile Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Arg Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 211
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 211
atgccgagcc gtgtgccggg tcgctgcatt atcttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgacgg ttatgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgcccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgctttgg agaacaaagt gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcacggcg gtt                              693

 
<210> 212
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 212
Met Pro Ser Arg Val Pro Gly Arg Cys Ile Ile Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Tyr Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Phe Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 213
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 213
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgtgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaaatg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacg 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgcactt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 214
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 214
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Met Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 215
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 215
atgccgagcc ccgtgactgg tcgatgcatt gagccggacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca gactggccag cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcatgc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taacgaaatg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaagcat tgcaagcatt aggtttcacg 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 216
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 216
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Glu Pro Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Asp Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Met Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Met Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 217
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 217
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatcagg aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 218
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 218
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Arg Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 219
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 219
atgccgagcc ccgtgactgg tcgacgcatt gagagcgacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca gactggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcatgc gcatgcgtgg ttggtacgac 240
atccagggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacg 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgtcggctcg cttcaccgcg gtt                              693

 
<210> 220
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 220
Met Pro Ser Pro Val Thr Gly Arg Arg Ile Glu Ser Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Asp Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Met Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Ser Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 221
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 221
atgccgagcc ccgtgactgg tcgacgcatt gagccggacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca gactggccag cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcatgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taacgaacaa gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttccat 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgtcggctcg cttcaccgcg gtt                              693

 
<210> 222
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 222
Met Pro Ser Pro Val Thr Gly Arg Arg Ile Glu Pro Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Asp Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Met Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe His Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Ser Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 223
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 223
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttaccgaact ggtccgtcca caatggccag ccttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 224
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 224
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Thr Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 225
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 225
atgccgagcc ccgtgactgg tcgatgcatt atcttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg catggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 226
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 226
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Ile Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp His Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 227
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 227
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg cgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcctgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcaatggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatcatctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 228
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 228
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Leu Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Met 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp His Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 229
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 229
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatcaac aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaacagtt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 230
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 230
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Asn Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Gln Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 231
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 231
atgccgagcc ctgtgccggg tcgctgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgacgg tagtgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgcccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgctttgg agaacaaagt gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcatggcg gtt                              693

 
<210> 232
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 232
Met Pro Ser Pro Val Pro Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Phe Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Met Ala Val                                     
225                 230     

 
<210> 233
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 233
atgccgagcc ccgtgactgg tcgatgtatt gggttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 234
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 234
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Gly Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 235
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 235
atgccgagcc ccgtgactgg tcgatgcatt atcttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctaacgg ttatgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgcccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgcagg agaacaaagt gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcatggcg gtt                              693

 
<210> 236
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 236
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Ile Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asn Gly Tyr Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Met Ala Val                                     
225                 230     

 
<210> 237
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 237
atgccgagcc gtgtgacggg tggctgcatt acgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctaacgg tagtgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgctttgg agaacaaagt gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcatggcg gtt                              693

 
<210> 238
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 238
Met Pro Ser Arg Val Thr Gly Gly Cys Ile Thr Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asn Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Phe Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Met Ala Val                                     
225                 230     

 
<210> 239
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 239
atgccgagcc ccgtgactgg tcgctgtatt atgttagaag tgattgaacg tgaaaccggg 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcatggcg gtt                              693

 
<210> 240
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 240
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Glu Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Met Ala Val                                     
225                 230     

 
<210> 241
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 241
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgcgttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatcaac aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcaccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 242
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 242
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Ala Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Asn Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Thr Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 243
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 243
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatcaac aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc ggggcgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 244
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 244
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Asn Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gly Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 245
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 245
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtcagtcca caatggccag cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgtgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaaatg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacg 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatcatctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 246
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 246
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Ser Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Met Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp His Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 247
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 247
atgccgagcc ccgtgactgg tcgatgcatt gagttagacg tgattgaaca cgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca gactggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgcactt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 248
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 248
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Glu Leu Asp Val Ile Glu 
1               5                   10                  15      
His Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Asp Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 249
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 249
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca gactggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 250
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 250
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Asp Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 251
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 251
atgccgagcc ccgtgactgg tcgatgcatt atgctggacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcctgc gcatgcgtgg ttggtacgac 240
atccagggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttccat 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaagctcg cttcaccgcg gtt                              693

 
<210> 252
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 252
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Leu Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe His Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 253
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 253
atgccgagcc ctgtgccggg tggctgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgacgg ttatgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgcccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgcagg agaacaaagt gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcacggcg gtt                              693

 
<210> 254
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 254
Met Pro Ser Pro Val Pro Gly Gly Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Tyr Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 255
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 255
atgccgagcc ctgtgccggg tcgctgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgacgg ttatgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgcccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgctttgg agaacaaagt gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcatggcg gtt                              693

 
<210> 256
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 256
Met Pro Ser Pro Val Pro Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Tyr Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Phe Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Met Ala Val                                     
225                 230     

 
<210> 257
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 257
atgccgagcc ccgtgactgg tcgatgtatt atgccggatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 258
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 258
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Pro Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 259
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 259
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgtagca 300
caagttgaag ctctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagc 480
caactgcaac cgcaagcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 260
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 260
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gln Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 261
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 261
atgccgagcc ccgtgactgg tggctgtatt acgttagatg tgattgaacg tgaaaccggg 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcacggcg gtt                              693

 
<210> 262
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 262
Met Pro Ser Pro Val Thr Gly Gly Cys Ile Thr Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 263
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 263
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatcaac aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaacgttt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 264
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 264
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Asn Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Arg Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 265
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 265
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg cgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttccat 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgcactt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 266
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 266
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe His Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 267
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 267
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 268
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 268
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 269
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 269
atgccgagcc gtgtgacggg tcgctgcatt acgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgacgg tagtgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgcccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgcagg agaacaaagt gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 270
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 270
Met Pro Ser Arg Val Thr Gly Arg Cys Ile Thr Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 271
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 271
atgccgagcc ccgtgactgg tcgaactatt gagccggacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca gactggccag cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcctgc gcatgcgtgg ttggtacgac 240
atccagggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taacgaacaa gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaagcat tgcaagcatt aggtttcacg 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaagctcg cttcaccgcg gtt                              693

 
<210> 272
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 272
Met Pro Ser Pro Val Thr Gly Arg Thr Ile Glu Pro Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Asp Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Leu Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 273
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 273
atgccgagcc ccgtgactgg tcgatgcatt atcttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtagtgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 274
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 274
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Ile Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ser Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 275
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 275
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatggacggt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatcaac aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 276
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 276
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Thr Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Asn Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 277
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 277
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgtgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgcactt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 278
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 278
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 279
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 279
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatcaac aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag atgcgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 280
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 280
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Asn Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Met Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 281
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 281
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatggggggt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatcaac aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 282
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 282
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Gly Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Asn Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 283
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 283
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatcaac aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcagtggcg cttcaccgcg gtt                              693

 
<210> 284
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 284
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Asn Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Trp Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 285
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 285
atgccgcgcc ccgtgactgg tggctgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcatggcg gtt                              693

 
<210> 286
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 286
Met Pro Arg Pro Val Thr Gly Gly Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Met Ala Val                                     
225                 230     

 
<210> 287
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 287
atgccgagcc ccgtgactgg tcgatgcatt gagttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 288
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 288
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Glu Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 289
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 289
atgccgagcc ccgtgactgg tcgatgcatt atcttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtggggatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 290
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 290
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Ile Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Gly Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 291
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 291
atgccgagcc ccgtgactgg tcgatgcatt gagttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca gactggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgcactt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 292
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 292
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Glu Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Asp Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 293
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 293
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaaca ggaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 294
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 294
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Gln Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 295
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 295
atgccgagcc ccgtgactgg tcgatgcatt gagttagacg tgattgaagt ggaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtcagtcca caatggccag cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttccat 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgcactt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 296
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 296
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Glu Leu Asp Val Ile Glu 
1               5                   10                  15      
Val Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Ser Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe His Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 297
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 297
atgccgagcc gtgtgccggg tcgctgcatt atcttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctaacgg tagtgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgcccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgcagg agaacaaagt gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcatggcg gtt                              693

 
<210> 298
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 298
Met Pro Ser Arg Val Pro Gly Arg Cys Ile Ile Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asn Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Met Ala Val                                     
225                 230     

 
<210> 299
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 299
atgcctagac ctgtaacagg tcgttgtatg attttggatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccagggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 300
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 300
Met Pro Arg Pro Val Thr Gly Arg Cys Met Ile Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 301
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 301
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc tcatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgcactt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 302
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 302
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala His Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 303
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 303
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaatg ggaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatcaac aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 304
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 304
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Trp Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Asn Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 305
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 305
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtcgcgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcatggcg gtt                              693

 
<210> 306
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 306
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Arg Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Met Ala Val                                     
225                 230     

 
<210> 307
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 307
atgccgagcc ctgtgacggg tcgctgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctaacgg tagtgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgcccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 308
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 308
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asn Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 309
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 309
atgccgagcc ccgtgactgg tcgctgtatt atgttagaag tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcacggcg gtt                              693

 
<210> 310
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 310
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Glu Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 311
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 311
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gggccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatcaac aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 312
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 312
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Gly Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Asn Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 313
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 313
atgccgagcc ccgtgactgg tcgctgtatt acgttagatg tgattgaacg tgaaaccggg 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgatttt taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcacggcg gtt                              693

 
<210> 314
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 314
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Thr Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Phe Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 315
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 315
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc tcatgaaatg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatcatctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 316
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 316
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala His Glu Met Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp His Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 317
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 317
atgccgagcc ccgtgactgg tcgatgcatt gagttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgtgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttccat 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 318
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 318
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Glu Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe His Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 319
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 319
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtcagtcca gactggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaaatg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaagcat tgcaagcatt aggtttcacg 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgcactt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 320
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 320
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Ser Pro Asp Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Met Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 321
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 321
atgccgagcc gtgtgacggg tggctgcatt acgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctaacgg ttatgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgcccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgcagg agaacaaagt gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcatggcg gtt                              693

 
<210> 322
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 322
Met Pro Ser Arg Val Thr Gly Gly Cys Ile Thr Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asn Gly Tyr Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Met Ala Val                                     
225                 230     

 
<210> 323
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 323
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
tttccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatcaac aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 324
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 324
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Phe Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Asn Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 325
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 325
atgccgagcc ctgtgacggg tggctgcatt acgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgacgg tagtgacttt 120
gcaccgatag ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgcccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgcagg agaacaaagt gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcacggcg gtt                              693

 
<210> 326
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 326
Met Pro Ser Pro Val Thr Gly Gly Cys Ile Thr Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Ile Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 327
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 327
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcaatggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatcatctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 328
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 328
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Met 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp His Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 329
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 329
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatcaac aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgatttt taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 330
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 330
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Asn Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Phe Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 331
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 331
atgccgagcc ccgtgactgg tcgatgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttccat 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatcatctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 332
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 332
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe His Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp His Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 333
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 333
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 334
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 334
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 335
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 335
atgccgagcc ccgtgactgg tcgaactatt gggctggacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca gactggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcctgc gcatgcgtgg ttggtacgac 240
atccagggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taacgaaatg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaagcat tgcaagcatt aggtttcacg 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 336
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 336
Met Pro Ser Pro Val Thr Gly Arg Thr Ile Gly Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Asp Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Leu Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Met Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 337
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 337
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaatc ggaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 338
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 338
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Ser Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 339
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 339
atgccgagcc ccgtgactgg tcgatgcatt gagttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgtgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaagcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgcactt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 340
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 340
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Glu Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 341
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 341
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagaagc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacccc 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 342
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 342
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Glu Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 343
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 343
atgccgagcc ccgtgactgg tcgctgtatt acgttagatg tgattgaacg tgaaaccggg 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcacggcg gtt                              693

 
<210> 344
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 344
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Thr Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 345
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 345
atgccgagcc ccgtgactgg tcgctgtatt acgttagatg tgattgaacg tgaaaccggg 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcacggcg gtt                              693

 
<210> 346
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 346
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Thr Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 347
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 347
atgccgagcc ccgtgactgg tcgatgcatt atcttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctaacgg ttatgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttatcccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgcagg agaacaaagt gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcatggcg gtt                              693

 
<210> 348
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 348
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Ile Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asn Gly Tyr Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Ile Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Met Ala Val                                     
225                 230     

 
<210> 349
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 349
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttcttggttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatcaac aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 350
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 350
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Trp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Asn Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 351
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 351
atgccgcgcc ccgtgactgg tggctgtatt acgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcatggcg gtt                              693

 
<210> 352
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 352
Met Pro Arg Pro Val Thr Gly Gly Cys Ile Thr Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Met Ala Val                                     
225                 230     

 
<210> 353
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 353
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatcaac aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaatcgtt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 354
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 354
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Asn Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ser Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 355
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 355
atgccgagcc ctgtgacggg tggctgcatt acgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgacgg ttatgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgcccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgcagg agaacaaagt gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcatggcg gtt                              693

 
<210> 356
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 356
Met Pro Ser Pro Val Thr Gly Gly Cys Ile Thr Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Tyr Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Met Ala Val                                     
225                 230     

 
<210> 357
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 357
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gattgggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 358
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 358
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Gly Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 359
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 359
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtcgcgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 360
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 360
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Arg Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 361
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 361
atgccgagcc ccgtgactgg tggctgtatt acgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcatggcg gtt                              693

 
<210> 362
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 362
Met Pro Ser Pro Val Thr Gly Gly Cys Ile Thr Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Met Ala Val                                     
225                 230     

 
<210> 363
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 363
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttcgtgaact ggtccgtcca caatggccag ccttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacccg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 364
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 364
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Arg Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 365
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 365
atgccgagcc ccgtgactgg tggctgtatt atgttagatg tgattgaacg tgaaaccggg 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcatggcg gtt                              693

 
<210> 366
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 366
Met Pro Ser Pro Val Thr Gly Gly Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Met Ala Val                                     
225                 230     

 
<210> 367
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 367
atgccgcgcc ccgtgactgg tcgctgtatt atgttagatg tgattgaacg tgaaaccggg 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 368
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 368
Met Pro Arg Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 369
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 369
atgccgcgcc ccgtgactgg tcgctgtatt acgttagatg tgattgaacg tgaaaccggg 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcatggcg gtt                              693

 
<210> 370
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 370
Met Pro Arg Pro Val Thr Gly Arg Cys Ile Thr Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Met Ala Val                                     
225                 230     

 
<210> 371
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 371
atgccgagcc ccgtgactgg tcgatgtatt atggtggatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 372
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 372
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Val Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 373
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 373
atgccgagcc ctgtgacggg tcgctgcatt acgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctaacgg ttatgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgcagg agaacaaagt gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcatggcg gtt                              693

 
<210> 374
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 374
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Thr Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asn Gly Tyr Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Met Ala Val                                     
225                 230     

 
<210> 375
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 375
atgccaagtc ctgttactgg tcgctggatg ctgctggatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgcttcggg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatcaac aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagcttccc ggccggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 376
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 376
Met Pro Ser Pro Val Thr Gly Arg Trp Met Leu Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Ser Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Asn Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Phe Pro Ala 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 377
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 377
atgccgagcc ccgtgactgg tggctgtatt acgttagaag tgattgaacg tgaaaccggg 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 378
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 378
Met Pro Ser Pro Val Thr Gly Gly Cys Ile Thr Leu Glu Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 379
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 379
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gatcgtgtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ctggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 380
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 380
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Arg Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 381
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 381
atgccgagcc ccgtgactgg tcgatgcatt atcttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgcttctgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 382
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 382
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Ile Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Ser Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 383
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 383
atgccgagcc ccgtgactgg tcgatgcatt atcttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc tagggaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 384
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 384
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Ile Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Arg Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 385
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 385
atgccgagcc ccgtgactgg tcgctgtatt atgttagatg tgattgaacg tgaaaccggg 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcacggcg gtt                              693

 
<210> 386
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 386
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 387
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 387
atgccgagcc ccgtgactgg tcgatgcatt atcttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctaatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagagtt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 388
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 388
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Ile Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asn Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Glu Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 389
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 389
atgccgagcc gtgtgacggg tcgctgcatt acgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctaacgg tagtgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgcccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgcagg agaacaaagt gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcatggcg gtt                              693

 
<210> 390
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 390
Met Pro Ser Arg Val Thr Gly Arg Cys Ile Thr Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asn Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Met Ala Val                                     
225                 230     

 
<210> 391
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 391
atgccgagcc ccgtgactgg tcgatgcatt atcttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg cggggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 392
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 392
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Ile Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Arg Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 393
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 393
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatcaac aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaagtg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 394
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 394
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Asn Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Val Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 395
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 395
atgccaagtc ctgttactgg tcgctggatg ctgctggatg tgattgaacg tgaaaccggt 60
ccaaacccta cctgggccgt gatttggctg catggcttgg gtgctgacgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatcaac aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgacctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 396
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 396
Met Pro Ser Pro Val Thr Gly Arg Trp Met Leu Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Thr Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Asn Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Asp Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 397
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 397
atgccgagcc ccgtgactgg tcgctgtatt acgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgatttt taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 398
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 398
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Thr Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Phe Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 399
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 399
atgccgcgcc ccgtgactgg tggctgtatt acgttagatg tgattgaacg tgaaaccggg 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcacggcg gtt                              693

 
<210> 400
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 400
Met Pro Arg Pro Val Thr Gly Gly Cys Ile Thr Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 401
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 401
atgccgcgcc ccgtgcctgg tcgatgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtcgcgca 300
caagttgaag ccctgattgc gaatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcacggcg gtt                              693

 
<210> 402
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 402
Met Pro Arg Pro Val Pro Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Arg Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 403
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 403
atgccgcgcc ccgtgactgg tggctgtatt acgttagaag tgattgaacg tgaaaccggg 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgatttt taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcacggcg gtt                              693

 
<210> 404
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 404
Met Pro Arg Pro Val Thr Gly Gly Cys Ile Thr Leu Glu Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Phe Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 405
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 405
atgccgagcc ccgtgactgg tcgaactatt gggagcgacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtcagtcca gactggccag cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcatgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taacgaacaa gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttccat 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgtcggctcg cttcaccgcg gtt                              693

 
<210> 406
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 406
Met Pro Ser Pro Val Thr Gly Arg Thr Ile Gly Ser Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Ser Pro Asp Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Met Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe His Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Ser Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 407
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 407
atgccgcgcc ccgtgactgg tggctgtatt atgttagaag tgattgaacg tgaaaccggg 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcatggcg gtt                              693

 
<210> 408
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 408
Met Pro Arg Pro Val Thr Gly Gly Cys Ile Met Leu Glu Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Met Ala Val                                     
225                 230     

 
<210> 409
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 409
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatcaac aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtatttttga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 410
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 410
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Asn Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Phe 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 411
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 411
atgccgagcc gtgtgacggg tcgctgcatt acgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctaacgg tagtgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgcccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgcagg agaacaaagt gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcacggcg gtt                              693

 
<210> 412
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 412
Met Pro Ser Arg Val Thr Gly Arg Cys Ile Thr Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asn Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 413
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 413
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatcaac aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagagg ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 414
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 414
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Asn Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Glu Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 415
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 415
atgccgagcc ccgtgactgg tcgatgcatt gagttagacg tgattgaatg cgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgtgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacg 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatcatctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 416
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 416
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Glu Leu Asp Val Ile Glu 
1               5                   10                  15      
Cys Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp His Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 417
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 417
atgccgagcc ccgtgactgg tcgatgcatt gagctggacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtcagtcca gactggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atcgcgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taacgaaatg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacg 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 418
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 418
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Glu Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Ser Pro Asp Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Ala Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Met Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 419
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 419
atgccgcgcc ccgtgactgg tggctgtatt atgttagatg tgattgaacg tgaaaccggg 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcatggcg gtt                              693

 
<210> 420
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 420
Met Pro Arg Pro Val Thr Gly Gly Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Met Ala Val                                     
225                 230     

 
<210> 421
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 421
atgccgcgcc ccgtgactgg tggctgtatt atgttagatg tgattgaacg tgaaaccggg 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcacggcg gtt                              693

 
<210> 422
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 422
Met Pro Arg Pro Val Thr Gly Gly Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 423
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 423
atgccgagcc ctgtgacggg tggctgcatt acgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctaacgg ttatgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgcagg agaacaaagt gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcacggcg gtt                              693

 
<210> 424
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 424
Met Pro Ser Pro Val Thr Gly Gly Cys Ile Thr Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asn Gly Tyr Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 425
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 425
atgccgagcc ccgtgactgg tggctgtatt acgttagaag tgattgaacg tgaaaccggg 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcacggcg gtt                              693

 
<210> 426
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 426
Met Pro Ser Pro Val Thr Gly Gly Cys Ile Thr Leu Glu Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 427
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 427
atgccgagcc ccgtgactgg tcgaactatt atgctggacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcctgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc tcacgaaatg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 428
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 428
Met Pro Ser Pro Val Thr Gly Arg Thr Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Leu Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala His Glu Met Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 429
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 429
atgccgagcc ccgtgactgg tcgatgcatt atcttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgtttcg 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 430
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 430
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Ile Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ser Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 431
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 431
atgccgagcc ccgtgactgg tcgatgcatt atcttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacgtt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 432
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 432
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Ile Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Val Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 433
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 433
atgccgagcc ctgtgacggg tcgctgcatt acgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctaacgg tagtgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgcccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgcagg agaacaaagt gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcatggcg gtt                              693

 
<210> 434
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 434
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Thr Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asn Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Met Ala Val                                     
225                 230     

 
<210> 435
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 435
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgtagca 300
caagttgaag ccctgatttt caatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcatggcg gtt                              693

 
<210> 436
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 436
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Phe Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Met Ala Val                                     
225                 230     

 
<210> 437
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 437
atgccgagcc ccgtgactgg tcgctgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgatttt taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcatggcg gtt                              693

 
<210> 438
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 438
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Phe Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Met Ala Val                                     
225                 230     

 
<210> 439
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 439
atgccgcgcc ccgtgactgg tggctgtatt atgttagaag tgattgaacg tgaaaccggg 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgatttt taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcacggcg gtt                              693

 
<210> 440
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 440
Met Pro Arg Pro Val Thr Gly Gly Cys Ile Met Leu Glu Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Phe Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 441
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 441
atgccgagcc ccgtgactgg tcgctgtatt atgttagatg tgattgaacg tgaaaccggg 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcatggcg gtt                              693

 
<210> 442
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 442
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Met Ala Val                                     
225                 230     

 
<210> 443
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 443
atgccgagcc ccgtgactgg tcgatgcatt atcttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgt gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 444
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 444
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Ile Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Val Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 445
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 445
atgccgagac cagttacggg gcgatgcata atattagagg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatcaac aatggcgttc gcatgcgtgg ttggtacgac 240
atccagggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 446
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 446
Met Pro Arg Pro Val Thr Gly Arg Cys Ile Ile Leu Glu Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Asn Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 447
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 447
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatcc gtctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatcaac aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 448
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 448
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Pro Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Asn Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 449
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 449
atgccgcgcc ccgtgactgg tggctgtatt acgttagaag tgattgaacg tgaaaccggg 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgatttt taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcatggcg gtt                              693

 
<210> 450
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 450
Met Pro Arg Pro Val Thr Gly Gly Cys Ile Thr Leu Glu Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Phe Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Met Ala Val                                     
225                 230     

 
<210> 451
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 451
atgccgagcc gcgtgcctgg tcgatgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcatggcg gtt                              693

 
<210> 452
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 452
Met Pro Ser Arg Val Pro Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Met Ala Val                                     
225                 230     

 
<210> 453
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 453
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatcaac aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcagggggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 454
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 454
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Asn Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Gly 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 455
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 455
atgccgagcc ccgtgactgg tcgaactatt atgccggacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atcgcgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaagcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgtcggctcg cttcaccgcg gtt                              693

 
<210> 456
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 456
Met Pro Ser Pro Val Thr Gly Arg Thr Ile Met Pro Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Ala Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Ser Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 457
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 457
atgccgagcc ccgtgactgg tcgatgcatt gggttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttccc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc tcatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaagcat tgcaagcatt aggtttcacg 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgcactt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 458
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 458
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Gly Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Pro Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala His Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 459
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 459
atgccgagcc ccgtgactgg tcgatgcatt gagctggacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtcagtcca cagtggccag cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taacgaaatg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaagcat tgcaagcatt aggtttcacg 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgtcggctcg cttcaccgcg gtt                              693

 
<210> 460
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 460
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Glu Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Ser Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Met Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Ser Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 461
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 461
atgccgagcc ccgtgactgg tggctgtatt acgttagatg tgattgaacg tgaaaccggg 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgatttt taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcatggcg gtt                              693

 
<210> 462
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 462
Met Pro Ser Pro Val Thr Gly Gly Cys Ile Thr Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Phe Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Met Ala Val                                     
225                 230     

 
<210> 463
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 463
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatcaac aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttatggg ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 464
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 464
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Asn Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Trp Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 465
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 465
atgccgagcc ccgtgactgg tcgaactatt gggctggacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca gactggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcctgc gcatgcgtgg ttggtacgac 240
atccagggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc tcacgaacaa gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttccat 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgtcggctcg cttcaccgcg gtt                              693

 
<210> 466
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 466
Met Pro Ser Pro Val Thr Gly Arg Thr Ile Gly Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Asp Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Leu Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala His Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe His Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Ser Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 467
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 467
atgccgagcc ccgtgactgg tcgatgtatt gagttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 468
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 468
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Glu Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 469
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 469
atgccgagcc gcgtgactgg tggctgtatt acgttagaag tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgatttt taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcatggcg gtt                              693

 
<210> 470
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 470
Met Pro Ser Arg Val Thr Gly Gly Cys Ile Thr Leu Glu Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Phe Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Met Ala Val                                     
225                 230     

 
<210> 471
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 471
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
tcgccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatcaac aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 472
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 472
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ser Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Asn Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 473
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 473
atgccgagcc ctgtgacggg tcgctgcatt acgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgacgg ttatgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgcccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgctttgg agaacaaagt gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcacggcg gtt                              693

 
<210> 474
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 474
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Thr Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Tyr Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Phe Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 475
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 475
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgatttt taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcacggcg gtt                              693

 
<210> 476
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 476
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Phe Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 477
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 477
atgccgagcc ccgtgactgg tcgatgcatt atcttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctaacgg ttatgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcacggcg gtt                              693

 
<210> 478
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 478
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Ile Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asn Gly Tyr Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 479
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 479
atgccgagcc gtgtgacggg tcgctgcatt acgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctaacgg tagtgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttatcccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgctttgg agaacaaagt gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 480
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 480
Met Pro Ser Arg Val Thr Gly Arg Cys Ile Thr Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asn Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Ile Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Phe Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 481
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 481
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgggccga ttcgtccgat cacgatcaac aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 482
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 482
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Gly Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Asn Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 483
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 483
atgccgagcc ccgtgactgg tggctgtatt atgttagatg tgattgaacg tgaaaccggg 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcacggcg gtt                              693

 
<210> 484
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 484
Met Pro Ser Pro Val Thr Gly Gly Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 485
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 485
atgccgagcc gtgtgacggg tcgctgcatt atcttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctaacgg ttatgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgcccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgcagg agaacaaagt gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcatggcg gtt                              693

 
<210> 486
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 486
Met Pro Ser Arg Val Thr Gly Arg Cys Ile Ile Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asn Gly Tyr Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Met Ala Val                                     
225                 230     

 
<210> 487
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 487
atgccgagcc ccgtgactgg tcgaactatt atgagcgacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca gactggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgtgc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taacgaacaa gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacg 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgtcggctcg cttcaccgcg gtt                              693

 
<210> 488
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 488
Met Pro Ser Pro Val Thr Gly Arg Thr Ile Met Ser Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Asp Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Ser Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 489
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 489
atgccgagcc ccgtgactgg tcgatgcatt atcttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atcgctggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 490
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 490
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Ile Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Ala Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 491
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 491
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatcaac aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat gaggtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 492
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 492
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Asn Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Glu Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 493
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 493
atgccgagcc ccgtgactgg tcgaactatt gagaacgacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtcagtcca cagtggccag cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcatgc gcatgcgtgg ttggtacgac 240
atccagggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 494
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 494
Met Pro Ser Pro Val Thr Gly Arg Thr Ile Glu Asn Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Ser Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Met Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 495
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 495
atgccgagcc ccgtgactgg tcgatgcatt atcttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccat ctttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 496
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 496
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Ile Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 497
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 497
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatcaac aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgttcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 498
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 498
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Asn Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ser Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 499
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 499
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatcaac aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggagcg cttcaccgcg gtt                              693

 
<210> 500
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 500
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Asn Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Glu Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 501
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 501
atgccgagcc ccgtgactgg tcgatgcatt atcttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccac cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 502
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 502
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Ile Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Pro Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 503
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 503
atgccgagcc ccgtgactgg tcgacgcatt atgctggacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca cagtggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 504
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 504
Met Pro Ser Pro Val Thr Gly Arg Arg Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 505
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 505
atgccgagcc ctgtgacggg tcgctgcatt atgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgacgg ttatgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgcagg agaacaaagt gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcacggcg gtt                              693

 
<210> 506
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 506
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Tyr Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 507
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 507
atgccaagtc ctgttactgg tcgctggatg ctgctggatg tgattgaacg tgaaaccggt 60
ccaaacccta cctgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatcaac aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 508
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 508
Met Pro Ser Pro Val Thr Gly Arg Trp Met Leu Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Thr Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Asn Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 509
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 509
atgccgagcc ccgtgactgg tcgctgtatt atgttagatg tgattgaacg tgaaaccggg 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcatggcg gtt                              693

 
<210> 510
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 510
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Met Ala Val                                     
225                 230     

 
<210> 511
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 511
atgccgagcc ccgtgactgg tcgatgcatt atcttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctattgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 512
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 512
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Ile Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Ile Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 513
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 513
atgccgagcc ccgtgactgg tggctgtatt acgttagaag tgattgaacg tgaaaccggg 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgatttt taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcatggcg gtt                              693

 
<210> 514
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 514
Met Pro Ser Pro Val Thr Gly Gly Cys Ile Thr Leu Glu Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Phe Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Met Ala Val                                     
225                 230     

 
<210> 515
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 515
atgccgagcc ccgtgactgg tcgatgcatt atcttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag atttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 516
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 516
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Ile Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Asp Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 517
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 517
atgccgagcc ccgtgactgg tgcgtgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatcaac aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 518
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 518
Met Pro Ser Pro Val Thr Gly Ala Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Asn Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 519
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 519
atgccgagcc gtgtgccggg tggctgcatt acgttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgacgg ttatgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgctttgg agaacaaagt gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcatggcg gtt                              693

 
<210> 520
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 520
Met Pro Ser Arg Val Pro Gly Gly Cys Ile Thr Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Tyr Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Phe Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Met Ala Val                                     
225                 230     

 
<210> 521
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 521
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatcaac aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt acgaggccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 522
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 522
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Asn Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Glu Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 523
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 523
atgccgagcc ccgtgactgg tcgacgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 524
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 524
Met Pro Ser Pro Val Thr Gly Arg Arg Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 525
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 525
atgccgagcc ccgtgactgg tcgatgtatt atgttagagg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatcaac aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctacca gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 526
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 526
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Glu Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Asn Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Gln Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 527
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 527
atgccgcgcc gtgtgccggg tcgctgcatt atcttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctaacgg ttatgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgcccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgcagg agaacaaagt gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcatggcg gtt                              693

 
<210> 528
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 528
Met Pro Arg Arg Val Pro Gly Arg Cys Ile Ile Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asn Gly Tyr Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Met Ala Val                                     
225                 230     

 
<210> 529
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 529
atgccgagcc ccgtgactgg tcgaactatt atgctggacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtcagtcca gactggccat cgttacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcatgc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc tcacgaaatg gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcaatggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggctcg cttcaccgcg gtt                              693

 
<210> 530
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 530
Met Pro Ser Pro Val Thr Gly Arg Thr Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Ser Pro Asp Trp Pro Ser Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Met Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala His Glu Met Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Met 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 531
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 531
atgccgagcc ccgtgactgg tcgatgcatt atcttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gctttccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 532
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 532
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Ile Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Leu Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 533
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 533
atgccgagcc ccgtgactgg tcgatgtatt atgttagatg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatcaac aatggcgttc gcatgcgtgg ttggtacgac 240
atcgtgggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagaccca 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcacc 600
cttgaatggc atagctaccc gatgggccat caagtgtgcg tagaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 534
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 534
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Met Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Asn Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Val Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Pro Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Thr Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Val Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

 
<210> 535
<211> 693
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 535
atgccgagcc ccgtgactgg tcgatgcatt atcttagacg tgattgaacg tgaaaccggt 60
ccaaaccccc aatgggccgt gatttggctg catggcttgg gtgctgatgg ttctgacttt 120
gcaccgatgg ttccggaact ggtccgtcca caatggccag cattacgctt cgtgtttcct 180
cacgcgccga ttcgtccgat cacgatctgg aatggcgttc gcatgcgtgg ttggtacgac 240
atccaaggga tggacttcgc gcaacgtgcg gataaggtgg gtattgcgga gagtgttgca 300
caagttgaag ccctgattgc taatgaacag gcacgcggga ttgctccaga tcgtattctg 360
ttggcgggct ttagtcaggg cggcgcggta acgttagcag ttggtcttca gcgtcgtgtt 420
ccattagcgg gcctgattgc gatgtccacc tacttaccag atccagccgc tgccgccagt 480
caactgcaac cgggtgcatt agcccagcca ttgttcatgg cacatggatc ggcagacctg 540
gttgttccgt accgcgccgg agaacaatca gcacaggcat tgcaagcatt aggtttcgcg 600
cttgaatggc atagctaccc gatgggccat caagtgtgct gggaggaaat cgatgccctt 660
cgtgattgga tgcaggcccg cttcaccgcg gtt                              693

 
<210> 536
<211> 231
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variants of Esterase  from  Xanthomonas campestris
 
<400> 536
Met Pro Ser Pro Val Thr Gly Arg Cys Ile Ile Leu Asp Val Ile Glu 
1               5                   10                  15      
Arg Glu Thr Gly Pro Asn Pro Gln Trp Ala Val Ile Trp Leu His Gly 
            20                  25                  30          
Leu Gly Ala Asp Gly Ser Asp Phe Ala Pro Met Val Pro Glu Leu Val 
        35                  40                  45              
Arg Pro Gln Trp Pro Ala Leu Arg Phe Val Phe Pro His Ala Pro Ile 
    50                  55                  60                  
Arg Pro Ile Thr Ile Trp Asn Gly Val Arg Met Arg Gly Trp Tyr Asp 
65                  70                  75                  80  
Ile Gln Gly Met Asp Phe Ala Gln Arg Ala Asp Lys Val Gly Ile Ala 
                85                  90                  95      
Glu Ser Val Ala Gln Val Glu Ala Leu Ile Ala Asn Glu Gln Ala Arg 
            100                 105                 110         
Gly Ile Ala Pro Asp Arg Ile Leu Leu Ala Gly Phe Ser Gln Gly Gly 
        115                 120                 125             
Ala Val Thr Leu Ala Val Gly Leu Gln Arg Arg Val Pro Leu Ala Gly 
    130                 135                 140                 
Leu Ile Ala Met Ser Thr Tyr Leu Pro Asp Pro Ala Ala Ala Ala Ser 
145                 150                 155                 160 
Gln Leu Gln Pro Gly Ala Leu Ala Gln Pro Leu Phe Met Ala His Gly 
                165                 170                 175     
Ser Ala Asp Leu Val Val Pro Tyr Arg Ala Gly Glu Gln Ser Ala Gln 
            180                 185                 190         
Ala Leu Gln Ala Leu Gly Phe Ala Leu Glu Trp His Ser Tyr Pro Met 
        195                 200                 205             
Gly His Gln Val Cys Trp Glu Glu Ile Asp Ala Leu Arg Asp Trp Met 
    210                 215                 220                 
Gln Ala Arg Phe Thr Ala Val                                     
225                 230     

