			SEQUENCE LISTING

<110> CODEXIS, INC.
 
<120> ENGINEERED TRANSAMINASE POLYPEPTIDES
 
<130> CX2-197WO1
 
<140> 
<141> 2021-04-06

<150> US 63/008,047
<151> 2020-04-10
 
 
<160> 936
 
<210> 1
<211> 1362
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Wild Type Transaminase from Vibrio fluvialis
 
<400> 1
atgaacaaac cgcagtcttg ggaggctcgt gcagaaactt actctctgta cggtttcacc 60
gacatgccgt ctctgcacca gcgtggcacc gtagttgtta cccacggcga aggtccgtac 120
atcgttgacg ttaacggtcg tcgttacctg gacgctaact ctggtctgtg gaacatggta 180
gctggtttcg accacaaagg tctgatcgac gctgctaaag ctcagtacga acgtttcccg 240
ggttaccacg ctttcttcgg tcgtatgtct gaccagaccg ttatgctgtc tgaaaaactg 300
gttgaagttt ctccgttcga ctctggtcgt gttttctaca ccaactctgg ttctgaagct 360
aacgacacta tggttaaaat gctgtggttc ctgcacgctg ctgaaggtaa accgcagaaa 420
cgtaaaatcc tgacccgttg gaacgcttac cacggcgtta ccgctgtttc tgcttctatg 480
accggtaaac cgtacaactc tgttttcggt ctgccgctgc cgggtttcgt tcacctgacc 540
tgcccgcact actggcgtta cggtgaagaa ggtgaaactg aagaacagtt cgttgctcgt 600
ctggctcgtg aactggaaga aaccatccag cgtgaaggtg ctgacaccat cgcaggtttc 660
ttcgctgaac cggttatggg tgctggtggt gttatcccgc cggctaaagg ttacttccag 720
gctatcctgc cgatcctgcg taaatacgac atcccggtta tctctgacga agttatctgc 780
ggtttcggtc gtaccggtaa cacctggggt tgcgttacct acgacttcac cccggacgct 840
atcatctctt ctaaaaacct gaccgctggt ttcttcccga tgggtgctgt tatcctgggt 900
ccggaactgt ctaaacgtct ggaaaccgct atcgaagcta tcgaagaatt cccgcacggt 960
ttcaccgctt ctggtcaccc ggttggttgc gctatcgctc tgaaagctat cgacgttgtt 1020
atgaacgaag gtctggctga aaacgtacgt cgtctggctc cgcgtttcga agaacgtctg 1080
aaacacatcg ctgaacgtcc gaacatcggt gaataccgtg gtatcggttt catgtgggct 1140
ctagaagctg ttaaagacaa agcatctaaa accccgttcg acggtaacct gtctgtttct 1200
gaacgtatcg ctaacacctg caccgacctg ggtctgatct gccgtccgct gggtcagtct 1260
gttgttctgt gcccgccgtt catcctgacc gaagctcaga tggacgaaat gttcgacaaa 1320
ctggaaaaag ctctggacaa agttttcgct gaagttgctt aa                    1362

 
<210> 2
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Wild Type Transaminase from Vibrio fluvialis
 
<400> 2
Met Asn Lys Pro Gln Ser Trp Glu Ala Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val Asn Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Phe Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Val Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Val His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Arg Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Met Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Ser Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Thr Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 3
<211> 1362
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 3
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                    1362

 
<210> 4
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 4
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 5
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 5
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg ctttgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 6
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 6
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 7
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 7
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 8
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 8
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 9
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 9
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaagc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaagagtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtcctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 10
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 10
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 11
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 11
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaagagtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg gcggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 12
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 12
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Gly Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 13
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 13
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaagc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 14
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 14
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 15
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 15
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaaa ctcagtatga acgttttccg 240
agctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 16
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 16
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Thr Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Ser Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 17
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 17
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaaa ctcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaagc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtcctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 18
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 18
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Thr Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 19
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 19
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcggtgaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 20
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 20
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Gly Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 21
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 21
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaatggtt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg gcggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 22
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 22
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Gly Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 23
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 23
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaagc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagagtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg acggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtcctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 24
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 24
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 25
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 25
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag ctcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgtggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg gcggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtcctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 26
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 26
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Val Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Gly Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 27
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 27
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaagc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 28
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 28
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 29
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 29
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagagtt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtcctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 30
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 30
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 31
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 31
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaagc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagagtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 32
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 32
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 33
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 33
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag ctcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 34
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 34
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 35
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 35
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaatggtt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg gcggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtcctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 36
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 36
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Gly Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 37
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 37
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaaa ctcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaagagtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 38
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 38
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Thr Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 39
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 39
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaagc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaagagtt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg gcggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 40
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 40
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Gly Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 41
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 41
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaagagtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg gcggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 42
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 42
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Gly Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 43
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 43
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaagagtt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg acggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtcctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 44
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 44
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 45
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 45
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 46
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 46
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 47
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 47
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg acggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 48
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 48
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 49
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 49
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaagagtt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 50
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 50
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 51
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 51
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaaa ctcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaagagtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg gcggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtcctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 52
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 52
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Thr Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Gly Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 53
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 53
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag ctcagtatga acgttttccg 240
ggctatcatg cgttttcggg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaagagtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg gcggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 54
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 54
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Gly Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 55
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 55
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag ctcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg gcggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 56
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 56
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Gly Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 57
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 57
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 58
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 58
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 59
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 59
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccagtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 60
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 60
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ser Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 61
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 61
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgtc tggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 62
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 62
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Ser Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 63
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 63
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 64
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 64
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 65
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 65
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg ctgggatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 66
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 66
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Trp Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 67
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 67
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 68
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 68
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 69
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 69
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 70
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 70
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 71
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 71
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaaa ctcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg gcggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 72
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 72
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Thr Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Gly Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 73
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 73
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cggtttcggg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 74
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 74
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Val Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 75
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 75
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagagtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg gcggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 76
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 76
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Gly Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 77
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 77
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaattttt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 78
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 78
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Phe Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 79
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 79
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaagc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg gcggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 80
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 80
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Gly Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 81
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 81
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaagc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg acggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 82
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 82
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 83
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 83
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaagc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 84
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 84
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 85
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 85
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtggtgtgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 86
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 86
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Val Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 87
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 87
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaaa ctcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaatggtt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg gcggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 88
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 88
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Thr Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Gly Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 89
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 89
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaaa ctcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaagc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg acggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 90
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 90
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Thr Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 91
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 91
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cggttgcggg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaagagtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg gcggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtcctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 92
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 92
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Val Ala Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Gly Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 93
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 93
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg acggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 94
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 94
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 95
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 95
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg gcggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 96
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 96
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Gly Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 97
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 97
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg acggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 98
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 98
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 99
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 99
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 100
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 100
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 101
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 101
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaaa ctcagtatga acgttttccg 240
ggctatcatg cgttttcggg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg acggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtcctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 102
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 102
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Thr Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 103
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 103
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcgccct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggctctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 104
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 104
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Ala Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Ala Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 105
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 105
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtcctct gggccagagc 1260
gtggctctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 106
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 106
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Ala Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 107
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 107
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
catatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 108
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 108
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 109
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 109
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg gcggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 110
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 110
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Gly Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 111
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 111
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcgccct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaagc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtcctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 112
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 112
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Ala Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 113
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 113
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcttgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 114
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 114
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 115
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 115
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gacatgtcga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgag tggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 116
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 116
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Ser Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Ser Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 117
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 117
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg acggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 118
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 118
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 119
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 119
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagggtt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 120
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 120
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Gly Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 121
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 121
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtgactctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 122
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 122
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Thr Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 123
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 123
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atgataacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgag tggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcacaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 124
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 124
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Asp Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Ser Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 125
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 125
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaaaattt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 126
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 126
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Asn Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 127
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 127
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccattg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgag tggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 128
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 128
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr His Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Ser Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 129
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 129
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 130
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 130
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 131
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 131
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 132
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 132
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 133
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 133
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaaa ctcagtatga acgttttccg 240
ggctatcatg cgttttcggg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaagc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg acggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 134
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 134
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Thr Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 135
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 135
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac agtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 136
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 136
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Gln Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 137
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 137
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atgataacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgag tggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagtacaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 138
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 138
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Asp Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Ser Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Val 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 139
<211> 1362
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 139
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcgccct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaaa ctcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaagc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gtgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                    1362

 
<210> 140
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 140
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Ala Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Thr Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Val Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 141
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 141
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaacattt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 142
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 142
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu His Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 143
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 143
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcgccct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag ctcagtatga acgttttccg 240
ggctatcatg cgtttgcggg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggctctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 144
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 144
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Ala Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ala Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Ala Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 145
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 145
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcgccct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag ctcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaagc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 146
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 146
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Ala Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 147
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 147
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atgataacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 148
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 148
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Asp Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 149
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 149
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag ctcagtatga acgttttccg 240
ggctatcatg cgttttcggg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaagc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg gcggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 150
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 150
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Gly Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 151
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 151
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg gcggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 152
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 152
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Gly Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 153
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 153
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gcgcgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 154
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 154
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Ala Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 155
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 155
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggctgcct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 156
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 156
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Cys Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 157
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 157
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaaa ctcagtatga acgttttccg 240
ggctatcatg cgtttgcggg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaagc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg gcggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtcctct gggccagagt 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 158
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 158
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Thr Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ala Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Gly Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 159
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 159
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaagc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 160
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 160
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 161
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 161
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaaaggtt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 162
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 162
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Arg Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 163
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 163
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgtgta gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccattg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgag tggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 164
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 164
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Cys Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr His Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Ser Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 165
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 165
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaagagtt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtcctct gggccagagc 1260
gtggctctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 166
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 166
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Ala Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 167
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 167
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaaa ctcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg gcggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 168
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 168
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Thr Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Gly Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 169
<211> 1362
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 169
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcgccct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                    1362

 
<210> 170
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 170
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Ala Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 171
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 171
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcgccct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag ctcagtatga acgttttccg 240
ggctatcatg cgtttgcggg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaagc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg gcggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtcctct gggccagagc 1260
gtggctctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 172
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 172
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Ala Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ala Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Gly Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Ala Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 173
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 173
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcgccct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaagc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 174
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 174
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Ala Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 175
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 175
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag ctcagtatga acgttttccg 240
ggctatcatg cgtttgcggg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaagc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg gcggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtcctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 176
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 176
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ala Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Gly Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 177
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 177
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcgccct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg gcggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtcctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 178
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 178
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Ala Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Gly Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 179
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 179
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
ccgatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgtgta gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgag tggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 180
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 180
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Pro Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Cys Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Ser Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 181
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 181
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag ctcagtatga acgttttccg 240
ggctatcatg cggttgcggg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagagtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg gcggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 182
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 182
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Val Ala Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Gly Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 183
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 183
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
agtcgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 184
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 184
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Ser Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 185
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 185
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccattg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atgataacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgag tggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 186
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 186
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr His Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Asp Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Ser Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 187
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 187
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg gcggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtcctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 188
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 188
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Gly Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 189
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 189
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaatggtt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg acggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtcctct gggccagagc 1260
gtggctctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 190
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 190
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Ala Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 191
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 191
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcgtttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 192
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 192
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Ala Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 193
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 193
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaaa ctcagtatga acgttttccg 240
ggctatcatg cgtttgcggg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaatggtt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg acggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 194
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 194
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Thr Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ala Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 195
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 195
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaaa ctcagtatga acgttttccg 240
ggctatcatg cggtttcggg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 196
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 196
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Thr Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Val Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 197
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 197
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag ctcagtatga acgttttccg 240
ggctatcatg cgttttcggg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaagc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 198
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 198
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 199
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 199
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgcgta gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 200
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 200
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Arg Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 201
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 201
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag ctcagtatga acgttttccg 240
ggctatcatg cgtttgcggg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg acggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtcctct gggccagagc 1260
gtggctctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 202
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 202
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ala Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Ala Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 203
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 203
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggctgcct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccattg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagtacaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 204
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 204
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Cys Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr His Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Val 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 205
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 205
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcacgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg ggcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 206
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 206
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Thr Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 207
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 207
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttggtgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 208
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 208
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Gly Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 209
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 209
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaaa ctcagtatga acgttttccg 240
ggctatcatg cggttgcggg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg gcggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 210
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 210
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Thr Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Val Ala Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Gly Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 211
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 211
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgttttcggg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaagc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtcctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 212
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 212
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 213
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 213
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgtgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgag tggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 214
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 214
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Val Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Ser Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 215
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 215
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcgccct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg gcggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 216
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 216
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Ala Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Gly Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 217
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 217
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gacatgtcga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgtgta gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgag tggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 218
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 218
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Ser Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Cys Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Ser Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 219
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 219
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagcg cgaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 220
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 220
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ala Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 221
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 221
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaaa ctcagtatga acgttttccg 240
ggctatcatg cgttttcggg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagagtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtcctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 222
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 222
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Thr Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 223
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 223
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaag attataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 224
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 224
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Asp Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 225
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 225
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaactcttat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 226
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 226
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ser Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 227
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 227
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgattact 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 228
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 228
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Thr Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 229
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 229
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtcctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 230
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 230
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 231
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 231
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cggtttcggg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaagc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 232
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 232
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Val Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 233
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 233
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaat cttataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 234
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 234
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Ser Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 235
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 235
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcgttct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 236
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 236
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Val Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 237
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 237
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggctgcct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atgataacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 238
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 238
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Cys Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Asp Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 239
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 239
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaaa ctcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagagtt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg gcggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 240
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 240
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Thr Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Gly Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 241
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 241
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag ctcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaagc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaagagtt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg acggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 242
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 242
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 243
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 243
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattt ctaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 244
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 244
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ser Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 245
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 245
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcgccct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag ctcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaagc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagagtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 246
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 246
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Ala Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 247
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 247
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcgccct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag ctcagtatga acgttttccg 240
ggctatcatg cgtttgcggg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaagc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaagagtt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 248
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 248
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Ala Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ala Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 249
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 249
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgtgta gcggctgcct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 250
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 250
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Cys Ser Gly Cys Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 251
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 251
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gacatgtcga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atgataacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgag tggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 252
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 252
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Ser Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Asp Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Ser Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 253
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 253
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtggc ggcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 254
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 254
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ala Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 255
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 255
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gacatgtcga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggctgcct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcacaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 256
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 256
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Ser Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Cys Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 257
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 257
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg cactatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 258
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 258
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly Thr Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 259
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 259
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtgtgg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 260
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 260
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Val Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 261
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 261
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatggcta gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 262
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 262
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Ala Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 263
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 263
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgag tggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagtacaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 264
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 264
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Ser Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Val 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 265
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 265
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgtgta gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcacaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 266
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 266
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Cys Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 267
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 267
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
ccgatgtcga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggctgcct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccattg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 268
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 268
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Pro Met Ser Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Cys Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr His Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 269
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 269
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gacatgtcga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgtgta gcggctgcct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccattg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atgataacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcacaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 270
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 270
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Ser Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Cys Ser Gly Cys Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr His Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Asp Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 271
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 271
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
ccgatgtcga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atgataacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgag tggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagtacaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 272
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 272
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Pro Met Ser Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Asp Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Ser Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Val 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 273
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 273
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
ccgatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgtgta gcggctgcct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 274
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 274
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Pro Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Cys Ser Gly Cys Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 275
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 275
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgtcg caacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atgataacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgag tggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagtacaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 276
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 276
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Arg Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Asp Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Ser Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Val 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 277
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 277
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ctcgatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 278
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 278
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly Ser Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 279
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 279
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtgcttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 280
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 280
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Leu Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 281
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 281
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
ccgatgtcga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccattg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccggt gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 282
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 282
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Pro Met Ser Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr His Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Val Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 283
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 283
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcagggaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 284
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 284
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Arg Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 285
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 285
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
ccgatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggctgcct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atgataacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 286
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 286
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Pro Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Cys Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Asp Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 287
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 287
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gacatgtcga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgtgta gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 288
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 288
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Ser Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Cys Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 289
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 289
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccggt gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagtacaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 290
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 290
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Val Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Val 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 291
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 291
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggctgcct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgtcg caacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 292
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 292
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Cys Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Arg Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 293
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 293
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaactgtt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 294
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 294
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Leu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 295
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 295
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
ccgatgtcga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggctgcct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccattg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagtacaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 296
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 296
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Pro Met Ser Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Cys Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr His Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Val 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 297
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 297
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcgctct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 298
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 298
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Ala Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 299
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 299
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gacatgtcga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggctgcct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atgataacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcacaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 300
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 300
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Ser Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Cys Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Asp Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 301
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 301
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagcttt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 302
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 302
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Ala Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 303
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 303
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccggc gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 304
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 304
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Ala Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 305
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 305
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttggatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 306
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 306
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Leu Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 307
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 307
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgtgta gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atgataacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcacaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 308
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 308
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Cys Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Asp Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 309
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 309
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gacatgtcga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgtgta gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atgataacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 310
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 310
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Ser Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Cys Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Asp Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 311
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 311
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggtttttgt 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 312
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 312
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Cys Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 313
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 313
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gacatgtcga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgtgta gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagtacaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 314
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 314
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Ser Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Cys Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Val 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 315
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 315
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
aagcgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 316
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 316
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Lys Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 317
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 317
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggctgcct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccattg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccggt gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 318
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 318
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Cys Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr His Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Val Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 319
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 319
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gacatgtcga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgtgta gcggctgcct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 320
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 320
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Ser Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Cys Ser Gly Cys Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 321
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 321
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccggt gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 322
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 322
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Val Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 323
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 323
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gcggtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 324
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 324
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Gly Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 325
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 325
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cgtatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 326
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 326
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Arg Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 327
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 327
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
ccgatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgag tggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagtacaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 328
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 328
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Pro Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Ser Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Val 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 329
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 329
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaag cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 330
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 330
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Ala Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 331
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 331
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgtggacctg caccgatctg ggcctgattt gtcgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 332
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 332
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Trp Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 333
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 333
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgtcg caacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgag tggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 334
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 334
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Arg Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Ser Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 335
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 335
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccattg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccggt gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 336
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 336
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr His Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Val Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 337
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 337
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccattg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 338
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 338
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr His Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 339
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 339
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaactcgtg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 340
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 340
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Ser Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 341
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 341
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atgataacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccggt gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 342
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 342
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Asp Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Val Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 343
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 343
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgcatacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 344
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 344
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala His Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 345
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 345
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gctggccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 346
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 346
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Trp Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 347
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 347
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtggat 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 348
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 348
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Asp 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 349
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 349
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gacatgtcga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgtcgatg 480
acgggtaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccggt gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagtacaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 350
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 350
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Ser Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Val Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Val 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 351
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 351
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccattg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atgataacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 352
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 352
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr His Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Asp Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 353
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 353
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagggg 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 354
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 354
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Gly Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 355
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 355
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagtttt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 356
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 356
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Val Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 357
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 357
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccatcg caacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atgataacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgag tggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 358
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 358
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr His Arg Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Asp Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Ser Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 359
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 359
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gacatgtcga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggctgcct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atgataacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccggt gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 360
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 360
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Ser Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Cys Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Asp Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Val Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 361
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 361
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gacatgtcga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaaacct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt 960
tttaccgcgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccggt gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 362
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 362
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Ser Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Ala Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     
Val Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 363
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 363
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cgcggatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 364
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 364
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Ala Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 365
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 365
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 366
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 366
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 367
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 367
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg tgggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg atcgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 368
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 368
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 369
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 369
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt ctgctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 370
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 370
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Leu Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 371
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 371
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgagacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttctttccgg tgggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 372
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 372
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Glu 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 373
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 373
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cgaggatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 374
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 374
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Glu Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 375
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 375
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgt 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 376
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 376
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 377
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 377
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cgagtcgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 378
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 378
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Ser Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 379
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 379
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacagattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 380
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 380
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys Gln Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 381
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 381
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgacggaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 382
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 382
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Thr Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 383
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 383
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga agctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 384
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 384
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Lys Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 385
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 385
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgtggaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 386
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 386
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Val Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 387
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 387
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atctttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttctttccgg tgggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatctgg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 388
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 388
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Phe Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Leu Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 389
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 389
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa gatgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 390
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 390
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Asp Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 391
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 391
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttctctaaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 392
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 392
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Ser Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 393
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 393
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa acttagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 394
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 394
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Leu Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 395
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 395
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggattc tgtgtttgcg gaagtggcg                        1359

 
<210> 396
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 396
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Ser Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 397
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 397
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atctttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgagacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg tgggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 398
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 398
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Phe Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Glu 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 399
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 399
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcatgcggg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 400
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 400
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Ala Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 401
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 401
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cgttgcgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 402
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 402
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Leu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 403
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 403
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 404
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 404
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 405
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 405
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatat gcgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 406
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 406
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Met Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 407
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 407
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaatcgg cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 408
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 408
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Ser Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 409
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 409
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cgcggcgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 410
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 410
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Arg Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 411
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 411
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttct gagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 412
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 412
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Leu Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 413
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 413
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc 60
gatatgcgta gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaactcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcttcc catataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 414
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 414
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Ala Phe Thr Asp Met Arg Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ser Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 415
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 415
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtccagcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 416
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 416
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Gln Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 417
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 417
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttag tccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 418
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 418
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Ser Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 419
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 419
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcattcgcg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 420
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 420
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Ser Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 421
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 421
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aatggcagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 422
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 422
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Trp Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 423
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 423
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atctttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgagacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg tgggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg atcgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatctgg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 424
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 424
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Phe Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Glu 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Leu Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 425
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 425
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cactgatctg ggcctgattg ttcgtgctag cggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 426
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 426
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Ser Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 427
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 427
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atctttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgagacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gtggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatctgg cggaacgtcc gaacattggc gaatatcgtg gcattggctt catgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatcac ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 428
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 428
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Phe Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Glu 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Leu Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp His Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 429
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 429
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atctttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgagacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacga gtggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatctgg cggaacatcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 430
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 430
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Phe Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Glu 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Ser Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Leu Ala Glu His Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 431
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 431
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaatttgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 432
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 432
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Phe Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 433
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 433
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgcttgataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 434
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 434
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Leu Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 435
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 435
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtga aggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg tgggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gtggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 436
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 436
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Glu Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 437
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 437
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtga aggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gtggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 438
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 438
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Glu Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 439
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 439
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggctgggaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 440
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 440
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Trp Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 441
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 441
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgacggataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 442
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 442
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Thr Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 443
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 443
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacggtg gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 444
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 444
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Gly Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 445
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 445
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cgcagcgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 446
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 446
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Gln Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 447
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 447
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattgtg aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 448
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 448
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Val Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 449
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 449
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg tggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 450
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 450
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Val Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 451
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 451
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgattacc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagccg tgaaagataa agcgagcaaa gccccgtttg atgacaacct gagcgtgagc 1200
agtcgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 452
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 452
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Thr Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Asp Asn Leu Ser Val Ser 
385                 390                 395                 400 
Ser Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 453
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 453
atgaacaaac cggggagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 454
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 454
Met Asn Lys Pro Gly Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 455
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 455
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa acctagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 456
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 456
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Pro Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 457
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 457
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgttttc gagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 458
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 458
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Ser Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 459
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 459
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc 60
gatatgcgta gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaactcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgattacc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagct cgaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agcgagcaaa gccccgtttg atgacaacct gagcgtgagc 1200
agtcgtattg cgaacacctg caccgatctg ggcctgattg ttcgtccact gggccagagc 1260
gtggtgctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 460
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 460
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Ala Phe Thr Asp Met Arg Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ser Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Thr Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Asp Asn Leu Ser Val Ser 
385                 390                 395                 400 
Ser Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 461
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 461
atgaacaaac cggagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 462
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 462
Met Asn Lys Pro Glu Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 463
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 463
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggctttacc 60
gatatgccta gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttggcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgt 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 464
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 464
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Gly Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 465
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 465
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga actgcgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 466
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 466
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Leu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 467
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 467
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg tcggtggcg                        1359

 
<210> 468
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 468
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Ser Val Ala                                             
    450                                                         

 
<210> 469
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 469
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggctctgaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 470
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 470
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Ser Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 471
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 471
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggctttgaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 472
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 472
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Phe Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 473
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 473
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccttg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 474
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 474
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Leu Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 475
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 475
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaagtgattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 476
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 476
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys Val Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 477
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 477
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggccctct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 478
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 478
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Pro Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 479
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 479
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaattgg cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 480
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 480
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Leu Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 481
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 481
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg cggttatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 482
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 482
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Gly Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 483
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 483
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattgct aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 484
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 484
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Ala Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 485
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 485
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggctttacc 60
gatatgcgta gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaactcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgt 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagcg cgaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agcgagcaaa gccccgtttg atgacaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 486
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 486
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Arg Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ser Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ala Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Asp Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 487
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 487
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacaa tgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 488
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 488
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Asn Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 489
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 489
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa acggcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 490
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 490
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Arg Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 491
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 491
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atcgtaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 492
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 492
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Arg Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 493
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 493
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccctttttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 494
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 494
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Leu Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 495
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 495
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattatg aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 496
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 496
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Met Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 497
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 497
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtccact gggccagagc 1260
gtggtgctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 498
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 498
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 499
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 499
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcggataaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 500
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 500
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Asp Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 501
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 501
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtggtctgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 502
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 502
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Val Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 503
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 503
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aggcatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 504
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 504
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Arg His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 505
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 505
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atctttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 506
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 506
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Phe Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 507
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 507
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttta tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 508
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 508
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Tyr Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 509
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 509
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggcct cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 510
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 510
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Ser Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 511
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 511
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgggttttt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 512
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 512
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Gly Phe Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 513
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 513
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtac taacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 514
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 514
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Thr Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 515
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 515
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggctatct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 516
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 516
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Tyr Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 517
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 517
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggatac tgtgtttgcg gaagtggcg                        1359

 
<210> 518
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 518
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Thr Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 519
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 519
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccatttat aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 520
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 520
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Tyr Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 521
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 521
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag agggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 522
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 522
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Arg Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 523
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 523
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccgc ggctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 524
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 524
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Arg Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 525
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 525
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg atcgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatctgg cggaacatcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 526
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 526
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Leu Ala Glu His Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 527
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 527
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtatta tgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 528
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 528
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Met Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 529
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 529
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcctgaaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 530
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 530
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Leu Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 531
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 531
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaagctgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 532
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 532
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Ala Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 533
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 533
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atctttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtga aggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctgaccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 534
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 534
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Phe Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Glu Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Thr Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 535
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 535
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atctttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgagacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gtggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatcac ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 536
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 536
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Phe Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Glu 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp His Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 537
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 537
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtga aggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgagacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttctttccgg tgggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacga gtggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatctgg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatcac ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 538
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 538
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Glu Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Glu 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Ser Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Leu Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp His Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 539
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 539
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgtggacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 540
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 540
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Trp Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 541
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 541
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa tttgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 542
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 542
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Phe Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 543
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 543
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcgagcgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 544
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 544
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Glu Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 545
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 545
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgagacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctgaccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 546
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 546
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Glu 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Thr Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 547
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 547
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
cagcgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 548
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 548
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Gln Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 549
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 549
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattttg aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 550
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 550
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Leu Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 551
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 551
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcagta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 552
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 552
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Gln Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 553
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 553
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgat gaagtggcg                        1359

 
<210> 554
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 554
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Asp Glu Val Ala                                             
    450                                                         

 
<210> 555
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 555
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaacgggcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 556
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 556
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Arg Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 557
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 557
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccgcttttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 558
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 558
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Ala Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 559
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 559
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtga aggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgagacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatctgg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 560
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 560
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Glu Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Glu 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Leu Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 561
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 561
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttggcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatga gttataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgattacc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 562
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 562
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Gly Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Ser Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Thr Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 563
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 563
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcttcg attataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgt 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaacattt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
agtcgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 564
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 564
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Asp Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu His Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Ser Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 565
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 565
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atctttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg tgggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatctgg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 566
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 566
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Phe Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Leu Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 567
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 567
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc 60
gatatgccta gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc aatataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggtgctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 568
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 568
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Ala Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Gln Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 569
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 569
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgagtggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg ctgggatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 570
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 570
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Trp Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 571
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 571
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggctttacc 60
gatatgcgta gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttggcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgt 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 572
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 572
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Arg Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Gly Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 573
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 573
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatcac ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 574
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 574
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp His Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 575
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 575
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgagacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatcac ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 576
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 576
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Glu 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp His Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 577
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 577
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggcaag gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtga aggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gtggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 578
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 578
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Lys Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Glu Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 579
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 579
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggctttacc 60
gatatgcgta gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggctgtct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttggcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaactcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgg attataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgt 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagct cgaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
agtcgtattg cgaacacctg caccgatctg ggcctgattg ttcgtccact gggccagagc 1260
gtggtgctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 580
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 580
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Arg Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Cys Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Gly Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ser Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Asp Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Ser Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 581
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 581
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatctgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgggttttt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 582
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 582
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Gly Phe Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 583
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 583
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtggtctgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgagtggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 584
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 584
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Val Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 585
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 585
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaactcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgt 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagct cgaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagccg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtccact gggccagagc 1260
gtggtgctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 586
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 586
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ser Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 587
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 587
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atctttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg tgggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagtgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatctgg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatcac ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 588
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 588
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Phe Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Val Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Leu Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp His Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 589
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 589
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atctttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctgaccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 590
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 590
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Phe Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Thr Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 591
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 591
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtggtctgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtat ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 592
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 592
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Val Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Met Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 593
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 593
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gtggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatctgg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 594
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 594
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Leu Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 595
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 595
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggctttacc 60
gatatgcgta gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaactcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgattacc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 596
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 596
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Arg Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ser Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Thr Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 597
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 597
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttggcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaactcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatga gttataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgt 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agcgagcaaa gccccgtttg atgacaacct gagcgtgagc 1200
agtcgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 598
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 598
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Gly Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ser Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Ser Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Asp Asn Leu Ser Val Ser 
385                 390                 395                 400 
Ser Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 599
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 599
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtccact gggccagagc 1260
gtgaccctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 600
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 600
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Thr Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 601
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 601
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atctttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggcaag gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 602
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 602
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Phe Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Lys Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 603
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 603
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atctttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg tgggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatctgg cggaacatcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 604
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 604
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Phe Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Leu Ala Glu His Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 605
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 605
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcttca gttataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgattacc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagct cgaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
agtcgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 606
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 606
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Ser Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Thr Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Ser Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 607
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 607
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtga aggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgagacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatctgg cggaacatcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatcac ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 608
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 608
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Glu Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Glu 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Leu Ala Glu His Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp His Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 609
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 609
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 610
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 610
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 611
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 611
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggcaag gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg atcgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 612
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 612
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Lys Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 613
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 613
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggctttacc 60
gatatgccta gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaactcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgattacc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagct cgaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 614
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 614
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ser Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Thr Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 615
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 615
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggctttacc 60
gatatgccta gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttggcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaactcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagccg tgaaagataa agcgagcaaa gccccgtttg atgacaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 616
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 616
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Gly Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ser Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Asp Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 617
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 617
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgagacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg tgggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatctgg cggaacatcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 618
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 618
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Glu 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Leu Ala Glu His Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 619
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 619
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atctttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtga aggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacga gtggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatctgg cggaacatcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 620
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 620
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Phe Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Glu Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Ser Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Leu Ala Glu His Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 621
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 621
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggcaag gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtga aggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccgctga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gtggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatatcg cggaacgtcc gaacattggc gaatatcgtg gcattggctt catgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatcac ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 622
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 622
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Lys Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Glu Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Leu Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp His Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 623
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 623
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgggtggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 624
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 624
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Gly Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 625
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 625
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgcgta gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc aatataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 626
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 626
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Arg Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Gln Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 627
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 627
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atctttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtga aggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgagacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 628
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 628
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Phe Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Glu Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Glu 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 629
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 629
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgcgta gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatga gttataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgt 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagct cgaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagccg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
agtcgtattg cgaacacctg caccgatctg ggcctgattg ttcgtccact gggccagagc 1260
gtggtgctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 630
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 630
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Arg Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Ser Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Ser Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Pro 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 631
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 631
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatctgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 632
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 632
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 633
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 633
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagccg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
agtcgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 634
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 634
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Ser Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 635
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 635
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagct cgaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gcacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 636
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 636
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Ala Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 637
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 637
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaactcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcttca gttataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgattacc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagct cgaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agcgagcaaa gccccgtttg atgacaacct gagcgtgagc 1200
gcacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggtgctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 638
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 638
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ser Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Ser Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Thr Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Asp Asn Leu Ser Val Ser 
385                 390                 395                 400 
Ala Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 639
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 639
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtga aggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccgctga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttaccacgg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatctgg cggaacatcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg caccgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 640
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 640
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Glu Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Leu Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Leu Ala Glu His Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 641
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 641
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga agctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcatgccgg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgt 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cgagccgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 642
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 642
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Lys Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Ala Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Ser Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 643
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 643
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga agctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcatgccgg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa gatgaaaccg aagaacagtt tgtccagcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 644
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 644
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Lys Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Ala Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Asp Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Gln Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 645
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 645
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtcgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaggg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa gacgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tattttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaccgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacaaattg cgagtcgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agctagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 646
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 646
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Asp Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Thr Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys Gln Ile Ala Ser Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 647
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 647
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaatgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agctagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg ctttgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 648
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 648
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 649
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 649
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 650
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 650
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 651
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 651
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga agctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg ctttgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 652
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 652
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Lys Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 653
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 653
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgt 780
ggctttggcc gcaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaatgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacaaattg cgagccgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg ctttgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 654
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 654
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys Gln Ile Ala Ser Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 655
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 655
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtcgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tattttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 656
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 656
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 657
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 657
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atctttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt ctgctggccc cgcgttttga agaacgtctg 1080
aaacaaattg cggagcgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agctagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cgcagatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 658
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 658
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Phe Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Leu Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys Gln Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Ala Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 659
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 659
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga aactgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaggg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tattttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agctagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 660
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 660
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Lys Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 661
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 661
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga aactgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtcgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcatgcggg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaccgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacacattg cgagtcgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cgaagatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 662
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 662
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Lys Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Ala Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Thr Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Ser Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Glu Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 663
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 663
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcatgccgg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agctagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 664
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 664
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Ala Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 665
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 665
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcatgccgg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacaaattg cgagccgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agctagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 666
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 666
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Ala Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys Gln Ile Ala Ser Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 667
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 667
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga agctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcatgccgg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggtgaaaccg aagaacagtt tgtcgcacgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgactgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg ctttgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 668
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 668
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Lys Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Ala Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Thr Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 669
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 669
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atctttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggtgaaaccg aagaacagtt tgtcgcacgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgt 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaatgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg ctttgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 670
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 670
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Phe Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 671
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 671
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcttcgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaggg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa gacgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacacattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cgaagatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 672
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 672
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Phe Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Asp Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Glu Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 673
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 673
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga agctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggtgaaaccg aagaacagtt tgtccagcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgattact 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttctttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaatgaag gcctggccga aaacgtgcgt ctgctggccc cgcgttttga agaacgtctg 1080
aaacaaattg cggagcgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agctagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg ctttgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 674
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 674
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Lys Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Gln Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Thr Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Leu Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys Gln Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 675
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 675
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga agctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcatgccgg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa gatgaaaccg aagaacagtt tgtcgcacgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttctttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaatgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacaaattg cggagcgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg ctttgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 676
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 676
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Lys Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Ala Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Asp Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys Gln Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 677
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 677
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga agctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgactgaag gcctggccga aaacgtgcgt ctgctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggagcgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agctagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 678
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 678
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Lys Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Thr Glu Gly Leu Ala Glu Asn Val Arg Leu Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 679
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 679
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcatgccgg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaatgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggagcgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg ctttgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 680
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 680
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Ala Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 681
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 681
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggtgaaaccg aagaacagtt tgtccagcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg ctttgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 682
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 682
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Gln Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 683
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 683
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga aactgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtcgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaggg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 684
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 684
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Lys Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 685
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 685
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtccagcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgattact 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttctttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggagcgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agctagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 686
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 686
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Gln Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Thr Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 687
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 687
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacaaattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agctagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 688
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 688
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys Gln Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 689
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 689
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttctttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agctagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg ctttgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 690
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 690
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 691
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 691
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga aactgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcttcgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcatgcggg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tattttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 692
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 692
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Lys Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Phe Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Ala Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 693
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 693
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaatgaag gcctggccga aaacgtgcgt ctgctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cgcagatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 694
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 694
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Leu Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Ala Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 695
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 695
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atctttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggtgaaaccg aagaacagtt tgtccagcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgt 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaatgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agctagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg ctttgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 696
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 696
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Phe Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Gln Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 697
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 697
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga agctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggtgaaaccg aagaacagtt tgtccagcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgattact 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttctttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacaaattg cggagcgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 698
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 698
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Lys Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Gln Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Thr Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys Gln Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 699
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 699
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga agctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttctttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaatgaag gcctggccga aaacgtgcgt ctgctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggagcgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agctagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 700
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 700
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Lys Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Leu Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 701
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 701
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga agctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgattact 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttctttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaatgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacaaattg cggagcgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg ctttgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 702
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 702
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Lys Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Thr Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys Gln Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 703
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 703
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttctttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggagcgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agctagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg ctttgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 704
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 704
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 705
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 705
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga agctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttct gagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa gatgaaaccg aagaacagtt tgtcgcacgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaatgaag gcctggccga aaacgtgcgt ctgctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggagcgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg ctttgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 706
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 706
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Lys Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Leu Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Asp Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Leu Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 707
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 707
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgt 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt ctgctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 708
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 708
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Leu Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 709
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 709
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgattact 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaatgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cgagccgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agctagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cgcagatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 710
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 710
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Thr Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Ser Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Ala Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 711
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 711
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa gatgaaaccg aagaacagtt tgtcgcacgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgattact 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggagcgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagccg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 712
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 712
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Asp Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Thr Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 713
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 713
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gcgtgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 714
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 714
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Val His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 715
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 715
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gcctgcatca gcgtggcacc gtggtgacga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 716
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 716
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Thr Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 717
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 717
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt ctgctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 718
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 718
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Leu Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 719
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 719
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa gatgaaaccg aagaacagtt tgtcgcacgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgt 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttctttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt ctgctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg ctttgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 720
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 720
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Asp Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Leu Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 721
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 721
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gggtgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 722
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 722
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Gly Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 723
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 723
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccgg agctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 724
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 724
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Glu Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 725
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 725
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcagttggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 726
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 726
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Ser Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 727
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 727
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga agctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcatgccgg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgattact 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaatgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cgcagatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 728
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 728
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Lys Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Ala Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Thr Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Ala Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 729
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 729
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agctagcaaa gccccgtttg atggtaacat gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 730
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 730
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Met Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 731
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 731
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cggctacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 732
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 732
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Ala Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 733
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 733
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggcagtagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 734
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 734
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Ser Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 735
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 735
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttct gagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa gatgaaaccg aagaacagtt tgtcgcacgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggagcgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agctagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 736
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 736
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Leu Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Asp Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 737
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 737
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttgttt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 738
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 738
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Val Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 739
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 739
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa gatgaaaccg aagaacagtt tgtccagcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgt 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttctttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaatgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cgagccgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg ctttgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 740
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 740
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Asp Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Gln Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Ser Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 741
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 741
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaccgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacaaattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agctagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 742
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 742
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Thr Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys Gln Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 743
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 743
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcatgccgg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa gatgaaaccg aagaacagtt tgtcgcacgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgt 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttctttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cgagccgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agctagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg ctttgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 744
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 744
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Ala Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Asp Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Ser Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 745
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 745
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gcctgcatca gtcgggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 746
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 746
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Ser Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 747
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 747
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttct gagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa gatgaaaccg aagaacagtt tgtccagcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttctttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cgagccgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg ctttgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 748
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 748
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Leu Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Asp Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Gln Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Ser Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 749
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 749
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga aactgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatat gcgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tattttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 750
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 750
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Lys Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Met Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 751
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 751
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtcgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcatgcggg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tattttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agctagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 752
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 752
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Ala Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 753
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 753
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga agctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 754
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 754
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Lys Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 755
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 755
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtcgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaggg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacaaattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 756
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 756
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys Gln Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 757
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 757
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga agctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcatgccgg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa gatgaaaccg aagaacagtt tgtcgcacgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgattact 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttctttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cgcagatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 758
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 758
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Lys Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Ala Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Asp Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Thr Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Ala Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 759
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 759
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcatgccgg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtccagcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt ctgctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agctagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 760
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 760
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Ala Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Gln Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Leu Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 761
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 761
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga agctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcatgccgg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa gatgaaaccg aagaacagtt tgtccagcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgattact 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaatgaag gcctggccga aaacgtgcgt ctgctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggagcgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 762
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 762
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Lys Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Ala Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Asp Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Gln Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Thr Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Leu Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 763
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 763
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga aactgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtcgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa gacgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tattttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacacattg cgagtcgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agctagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 764
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 764
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Lys Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Asp Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Ser Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 765
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 765
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcga aggaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 766
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 766
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Lys Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 767
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 767
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga agctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttct gagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt ctgctggccc cgcgttttga agaacgtctg 1080
aaacatattg cgagccgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagccg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 768
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 768
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Lys Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Leu Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Leu Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Ser Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 769
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 769
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtcgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaggg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa gacgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tattttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 770
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 770
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Asp Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 771
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 771
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga agctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cgcagatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 772
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 772
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Lys Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Ala Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 773
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 773
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtcgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaggg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tattttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacacattg cgagtcgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 774
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 774
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Ser Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 775
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 775
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatat gcgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttctttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaatgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 776
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 776
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Met Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 777
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 777
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gccatcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 778
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 778
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser His His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 779
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 779
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga agctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atctttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaatgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacaaattg cggagcgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 780
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 780
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Lys Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Phe Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys Gln Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 781
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 781
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tattttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacaaattg cgagtcgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 782
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 782
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys Gln Ile Ala Ser Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 783
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 783
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgtctggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 784
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 784
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro Ser Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 785
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 785
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atctttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcacgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgt 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaatgaag gcctggccga aaacgtgcgt ctgctggccc cgcgttttga agaacgtctg 1080
aaacaaattg cgagccgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 786
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 786
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Phe Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Leu Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys Gln Ile Ala Ser Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 787
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 787
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgattact 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 788
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 788
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Thr Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 789
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 789
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgattact 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttctttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cgagccgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agctagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 790
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 790
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Thr Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Ser Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 791
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 791
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatat gcgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 792
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 792
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Met Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 793
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 793
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgt 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaatgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg ctttgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 794
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 794
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 795
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 795
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcatgccgg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggccatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggtgaaaccg aagaacagtt tgtcgcacgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgt 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agctagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg ctttgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 796
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 796
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Ala Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly His His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 797
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 797
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga agctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgt 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaatgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacaaattg cggagcgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagccg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg ctttgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 798
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 798
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Lys Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys Gln Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 799
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 799
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gcctgcatca gactggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 800
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 800
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Thr Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 801
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 801
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggctcta cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 802
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 802
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 803
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 803
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaaccattg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 804
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 804
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn His Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 805
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 805
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttactgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 806
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 806
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Thr Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 807
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 807
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaagtttt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 808
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 808
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Val Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 809
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 809
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgctgg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 810
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 810
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Leu Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 811
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 811
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt ttttttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagcgg tgaaagataa agcgagcaaa gccccgtttg atggcaggct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 812
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 812
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 
Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Arg Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 813
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 813
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaactgg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 814
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 814
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Leu Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 815
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 815
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga agcgtttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 816
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 816
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Ala Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 817
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 817
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatcgtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 818
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 818
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Ser Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 819
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 819
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaactttt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 820
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 820
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Leu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 821
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 821
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaaaattt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 822
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 822
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Asn Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 823
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 823
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggggaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 824
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 824
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Gly Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 825
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 825
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgcaggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggtc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 826
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 826
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Gln Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 827
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 827
atgaacaaac cgcagagctg ggaaacgcgt gcggaaaccg ctagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 828
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 828
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Ala Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 829
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 829
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtggttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 830
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 830
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Val Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 831
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 831
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cggactttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaattttt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 832
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 832
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Asp Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Phe Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 833
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 833
atgaacaaac cgcagagctg ggaaacgcgt gcggaaactc acagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tcgtggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgctcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg agtgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtc 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagttg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 834
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 834
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr His Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Arg Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ser Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 835
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 835
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttatg ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 836
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 836
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Ala Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 837
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 837
atgaacaaac cgcagagctg ggaaacgcgt gcggaaactt acagcctgta tggttttacc 60
cacatgccgg aactgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagtggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgccg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtccagcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtc 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagttg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 838
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 838
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Glu Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Gln Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 839
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 839
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaagattt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 840
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 840
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Asp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 841
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 841
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgattctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 842
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 842
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Ile Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 843
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 843
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcgt cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 844
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 844
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Ser Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 845
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 845
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatattt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 846
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 846
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Tyr Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 847
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 847
atgaacaaac cgcagagctg ggaaacgcgt gcggaaaccg gtagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 848
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 848
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Gly Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 849
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 849
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggatgtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 850
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 850
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Asp Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 851
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 851
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcccgtg cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 852
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 852
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Arg Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 853
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 853
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaagtttt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 854
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 854
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Val Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 855
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 855
atgaacaaac cgcagagctg ggaaacgcgt gcggaaactc acagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagtggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgccg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgctcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg agtgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtc 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagttg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 856
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 856
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr His Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ser Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 857
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 857
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg aaggaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 858
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 858
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Lys Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 859
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 859
atgaacaaac cgcagagctg ggaaacgcgt gcggaaactt acagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tcgtggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgccg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgctcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 860
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 860
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Arg Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 861
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 861
atgaacaaac cgcagagctg ggaaacgcgt gcggaagctt acagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagtggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgccg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgctcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg agtgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtc 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagttg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 862
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 862
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Ala Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ser Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 863
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 863
atgaacaaac cgcagagctg ggaaacgcgt gcggaagctt acagcctgta tggttttacc 60
cacatgccga aactgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagtggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgccg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtccagcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg agtgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtc 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagttg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 864
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 864
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Ala Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Lys Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Gln Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ser Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 865
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 865
atgaacaaac cgcagagctg ggaaacgcgt gcggaagctt acagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tcgtggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgctcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagttg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 866
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 866
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Ala Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Arg Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 867
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 867
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggcctctg cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 868
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 868
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Ser Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 869
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 869
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagctaa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 870
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 870
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Ala Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 871
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 871
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga ccgagggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 872
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 872
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr Glu Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 873
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 873
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtgtggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 874
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 874
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Cys Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 875
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 875
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggaga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
ctggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 876
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 876
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Leu Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 877
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 877
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaagagtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 878
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 878
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Glu Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 879
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 879
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttgcgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 880
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 880
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Ala Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 881
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 881
atgaacaaac cgcagagctg ggaaacgcgt gcggaaactt acagcctgta tggttttacc 60
cacatgccgg aactgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagtggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgctcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtc 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagttg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 882
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 882
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Glu Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 883
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 883
atgaacaaac cgcagagctg ggaaacgcgt gcggaagctt acagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagtggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtc 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagttg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 884
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 884
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Ala Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 885
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 885
atgaacaaac cgcagagctg ggaaacggag gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 886
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 886
Met Asn Lys Pro Gln Ser Trp Glu Thr Glu Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 887
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 887
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcgtgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 888
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 888
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Val Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 889
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 889
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaagggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 890
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 890
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Gly Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 891
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 891
atgaacaaac cgcagagctg ggaaacgcgt gcggaaactc acagcctgta tggttttacc 60
cacatgccga aactgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tcgtggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtccagcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagttg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 892
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 892
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr His Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Lys Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Arg Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Gln Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 893
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 893
atgaacaaac cgcagagctg ggaaacgcgt gcggaagctt acagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagtggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgccg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtccagcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtc 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagttg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 894
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 894
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Ala Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Gln Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 895
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 895
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaacattt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 896
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 896
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu His Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 897
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 897
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaaatttt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 898
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 898
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Ile Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 899
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 899
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaattttt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 900
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 900
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Phe Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 901
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 901
atgaacaaac cgcagagctg ggaaacgcgt gcggaaactc acagcctgta tggttttacc 60
cacatgccga aactgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgccg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtccagcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtc 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 902
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 902
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr His Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Lys Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Gln Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 903
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 903
atgaacaaac cgcagagctg ggaaacgcgt gcggaagctt acagcctgta tggttttacc 60
cacatgccgg aactgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tcgtggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgccg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgctcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg agtgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtc 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagttg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 904
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 904
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Ala Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Glu Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Arg Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ser Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 905
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 905
atgaacaaac cgcagagctg ggaaacgcgt gcggaaactc acagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgccg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 906
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 906
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr His Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 907
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 907
atgaacaaac cgcagagctg ggaaacgcgt gcggaagctt acagcctgta tggttttacc 60
cacatgccgg aactgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagtggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgccg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag tgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgctcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagttg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 908
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 908
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Ala Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Glu Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 909
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 909
atgaacaaac cgcagagctg ggaaacgcgt gcggaagctt acagcctgta tggttttacc 60
cacatgccgg aactgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagtggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtccagcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg agtgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtc 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagttg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 910
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 910
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Ala Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Glu Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Gln Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ser Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 911
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 911
atgaacaaac cgcagagctg ggaaacgcgt gcggaaactt acagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tcgtggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgccg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtccagcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 912
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 912
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Arg Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Gln Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 913
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 913
atgaacaaac cgcagagctg ggaaacgcgt gcggaaactc acagcctgta tggttttacc 60
cacatgccgg aactgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tcgtggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgctcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtc 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagttg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 914
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 914
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr His Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Glu Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Arg Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 915
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 915
atgaacaaac cgcagagctg ggaaacgcgt gcggaaactc acagcctgta tggttttacc 60
cacatgccga aactgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tcgtggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgccg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgctcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtc 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagttg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 916
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 916
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr His Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Lys Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Arg Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 917
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 917
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggccatc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 918
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 918
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly His Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 919
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 919
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccgg aactgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgccg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgctcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagttg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 920
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 920
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Glu Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 921
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 921
atgaacaaac cgcagagctg ggaaacgcgt gcggaaactc acagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tcgtggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgctcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtc 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagttg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 922
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 922
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr His Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Arg Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 923
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 923
atgaacaaac cgcagagctg ggaaacgcgt gcggaagctt acagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tcgtggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgccg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg agtgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtc 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagttg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 924
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 924
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Ala Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Arg Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ser Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 925
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 925
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgccg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtccagcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg agtgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 926
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 926
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Gln Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ser Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 927
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 927
atgaacaaac cgcagagctg ggaaacgcgt gcggaaactc acagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tcgtggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgccg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgctcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg agtgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtc 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagttg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 928
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 928
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr His Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Arg Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ser Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 929
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 929
atgaacaaac cgcagagctg ggaaacgcgt gcggaagctt acagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tcgtggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtccagcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagttg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 930
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 930
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Ala Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Arg Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Gln Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 931
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 931
atgaacaaac cgcagagctg ggaaacgcgt gcggaaactt acagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagtggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgccg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcatgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtccagcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtc 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 932
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 932
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Met Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Gln Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 933
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 933
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tggttttacc 60
cacatgccga gtctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggctcgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagtcg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 934
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 934
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Ser Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

 
<210> 935
<211> 1359
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 935
atgaacaaac cgcagagctg ggaaacgcgt gcggaagctt acagcctgta tggttttacc 60
cacatgccga aactgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat 120
atcgttgatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgct gaacatggtg 180
gcgggctttg atcataaagg cctgattgat gcggccaaag cgcagtatga acgttttccg 240
ggctatcatg cgtttagcgg ccatatgagc gatcagaccg tgatgctgtc tgaaaaactg 300
gtggaagtga gcccgtttga tcgtggccgt gtgttttata ccaacagcgg cagcgaagcg 360
aacgatacca tggtgaaaat gctgtggttt ctgcatgccg cggaaggcaa accgcagaaa 420
cgtaaaattc tgacccgttg gaacgcgtat catggcgcga cggcggtgag cgcgagcatg 480
accggcctgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc 540
tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt 600
ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt 660
tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag 720
gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc 780
ggctttggcc gtaccggtaa cacctggggc tgcgtgacct atgattttac cccggatgcg 840
attattagca gtaaaagcct aaccgcgggt tactttccgg taggcgcggt gattctgggt 900
ccggaactga gcaaacgtct ggaaaccgcg attgaagcgc gcgaatggtt tccgcatggt 960
tttacctgtg gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg 1020
atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg 1080
aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg 1140
ctggaagttg tgaaagataa agatagcaaa gccccgtttg atggcaacct gagcgtgagc 1200
gaacgtattg cgaacacctg cttcgatctg ggcctgattg ttcgtgctct gggccagagc 1260
gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa 1320
ctggaaaaag cgctggataa agtgtttgcg gaagtggcg                        1359

 
<210> 936
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Transaminase from Vibrio fluvialis
 
<400> 936
Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Ala Tyr Ser Leu 
1               5                   10                  15      
Tyr Gly Phe Thr His Met Pro Lys Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              
Tyr Leu Asp Ala Asn Ser Gly Leu Leu Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  
Gly Tyr His Ala Phe Ser Gly His Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Arg Gly Arg Val Phe 
            100                 105                 110         
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 
Thr Arg Trp Asn Ala Tyr His Gly Ala Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 
Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     
Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             
Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Ser Leu Thr 
        275                 280                 285             
Ala Gly Tyr Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 
Lys Arg Leu Glu Thr Ala Ile Glu Ala Arg Glu Trp Phe Pro His Gly 
305                 310                 315                 320 
Phe Thr Cys Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 
Lys Asp Lys Asp Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 
Glu Arg Ile Ala Asn Thr Cys Phe Asp Leu Gly Leu Ile Val Arg Ala 
                405                 410                 415     
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             
Phe Ala Glu Val Ala                                             
    450                                                         

