                         SEQUENCE LISTING

<110>  SHENZHEN HUA YAO KANG MING BIOPHARMACEUTICAL CO., LTD.
 
<120>  ENGINEERED ONCOLYTIC ADENOVIRUS

<130>  0237-PA-002

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  2147
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence for deletion of E3gp19k

<400>  1
gatatcatta catcaagatc tttgttgcca tctctgtgct gagtataata aatacagaaa       60

ttaaaatata ctggggctcc tatcgccatc ctgtaaacgc caccgtcttc acccgcccaa      120

gcaaaccaag gcgaacctta cctggtactt ttaacatctc tccctctgtg atttacaaca      180

gtttcaaccc agacggagtg agtctacgag agaacctctc cgagctcagc tactccatca      240

gaaaaaacac caccctcctt acctgccggg aacgtacgag tgcgtcaccg gccgctgcac      300

cacacctacc gcctgaccgt aaaccagact ttttccggac agacctcaat aactctgttt      360

accagaacag gaggtgagct tagaaaaccc ttagggtatt aggccaaagg cgcagctact      420

gtggggttta tgaacaattc aagcaactct acgggctatt ctaattcagg tttctctaga      480

atcggggttg gggttattct ctgtcttgtg attctcttta ttcttatact aacgcttctc      540

tgcctaaggc tcgccgcctg ctgtgtgcac atttgcattt attgtcagct ttttaaacgc      600

tggggtcgcc acccaagatg atttaaatat acctgtgacg gaagatcact tcgcagaata      660

aataaatcct ggtgtccctg ttgataccgg gaagccctgg gccaactttt ggcgaaaatg      720

agacgttgat cggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc      780

gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa tggagaaaaa      840

aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc      900

atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt      960

tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc     1020

ccgcctgatg aatgctcatc cggagttccg tatggcaatg aaagacggtg agctggtgat     1080

atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc     1140

gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt     1200

ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt     1260

cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga     1320

caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct     1380

gatgccgctg gcgattcagg ttcatcatgc cgtctgtgat ggcttccatg tcggcagaat     1440

gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaat ttttttaagg     1500

cagttattgg tggccttaaa cgcctattta aattttacta agttacaaag ctaatgtcac     1560

cactaactgc tttactcgct gcttgcaaaa caaattcaaa aagttagcat tataattaga     1620

ataggattta aaccccccgg tcatttcctg ctcaatacca ttcccctgaa caattgactc     1680

tatgtgggat atgctccagc gctacaacct tgaagtcagg cttcctggat gtcagcatct     1740

gactttggcc agcacctgtc ccgcggattt gttccagtcc aactacagcg acccacccta     1800

acagagatga ccaacacaac caacgcggcc gccgctaccg gacttacatc taccacaaat     1860

acaccccaag tttctgcctt tgtcaataac tgggataact tgggcatgtg gtggttctcc     1920

atagcgctta tgtttgtatg ccttattatt atgtggctca tctgctgcct aaagcgcaaa     1980

cgcgcccgac cacccatcta tagtcccatc attgtgctac acccaaacaa tgatggaatc     2040

catagattgg acggactgaa acacatgttc ttttctctta cagtatgatt aaatgagaca     2100

tgattcctcg agtttttata ttactgaccc ttgttgcgct tgatatc                   2147


<210>  2
<211>  3184
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence for inserting human IL-21 into the E3gp19k region

<400>  2
gatatcgact gaatgttaag tggagaggca gagcaactgc gcctgaaaca cctggtccac       60

tgtcgccgcc acaagtgctt tgcccgcgac tccggtgagt tttgctactt tgaattgccc      120

gaggatcata tcgagggccc ggcgcacggc gtccggctta ccgcccaggg agagcttgcc      180

cgtagcctga ttcgggagtt tacccagcgc cccctgctag ttgagcggga caggggaccc      240

tgtgttctca ctgtgatttg caactgtcct aaccctggat tacatcaaga tctttgttgc      300

catctctgtg ctgagtataa taaatacaga aattaaaata tactggggct cctatcgcca      360

tcctgtaaac gccaccgtct tcacccgccc aagcaaacca aggcgaacct tacctggtac      420

ttttaacatc tctccctctg tgatttacaa cagtttcaac ccagacggag tgagtctacg      480

agagaacctc tccgagctca gctactccat cagaaaaaac accaccctcc ttacctgccg      540

ggaacgtacg agtgcgtcac cggccgctgc accacaccta ccgcctgacc gtaaaccaga      600

ctttttccgg acagacctca ataactctgt ttaccagaac aggaggtgag cttagaaaac      660

ccttagggta ttaggccaaa ggcgcagcta ctgtggggtt tatgaacaat tcaagcaact      720

ctacgggcta ttctaattca ggtttctcta gaatcggggt tggggttatt ctctgtcttg      780

tgattctctt tattcttata ctaacgcttc tctgcctaag gctcgccgcc tgctgtgtgc      840

acatttgcat ttattgtcag ctttttaaac gctggggtcg ccacccaaga tgagatccag      900

tcctggcaac atggagagga ttgtcatctg tctgatggtc atcttcttgg ggacactggt      960

ccacaaatca agctcccaag gtcaagatcg ccacatgatt agaatgcgtc aacttataga     1020

tattgttgat cagctgaaaa attatgtgaa tgacttggtc cctgaatttc tgccagctcc     1080

agaagatgta gagacaaact gtgagtggtc agctttttcc tgctttcaga aggcccaact     1140

aaagtcagca aatacaggaa acaatgaaag gataatcaat gtatcaatta aaaagctgaa     1200

gaggaaacca ccttccacaa atgcagggag aagacagaaa cacagactaa catgcccttc     1260

atgtgattct tatgagaaaa aaccacccaa agagttccta gaaagattca aatcacttct     1320

ccaaaagatg attcatcagc atctgtcctc tagaacacac ggaagtgaag attcctgaat     1380

ttaaatatac ctgtgacgga agatcacttc gcagaataaa taaatcctgg tgtccctgtt     1440

gataccggga agccctgggc caacttttgg cgaaaatgag acgttgatcg gcacgtaaga     1500

ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt tgagttatcg     1560

agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt     1620

gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt     1680

acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat     1740

aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg     1800

gagttccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt     1860

tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac     1920

gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg     1980

gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg     2040

agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc     2100

accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt     2160

catcatgccg tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac     2220

tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca gttattggtg gccttaaacg     2280

cctatttaaa ttttactaag ttacaaagct aatgtcacca ctaactgctt tactcgctgc     2340

ttgcaaaaca aattcaaaaa gttagcatta taattagaat aggatttaaa ccccccggtc     2400

atttcctgct caataccatt cccctgaaca attgactcta tgtgggatat gctccagcgc     2460

tacaaccttg aagtcaggct tcctggatgt cagcatctga ctttggccag cacctgtccc     2520

gcggatttgt tccagtccaa ctacagcgac ccaccctaac agagatgacc aacacaacca     2580

acgcggccgc cgctaccgga cttacatcta ccacaaatac accccaagtt tctgcctttg     2640

tcaataactg ggataacttg ggcatgtggt ggttctccat agcgcttatg tttgtatgcc     2700

ttattattat gtggctcatc tgctgcctaa agcgcaaacg cgcccgacca cccatctata     2760

gtcccatcat tgtgctacac ccaaacaatg atggaatcca tagattggac ggactgaaac     2820

acatgttctt ttctcttaca gtatgattaa atgagacatg attcctcgag tttttatatt     2880

actgaccctt gttgcgcttt ttttgtgcgt gctccacatt ggctgcggtt tctcacatcg     2940

aagtagactg cattccagcc ttcacagtct atttgcttta cggatttgtc accctcacgc     3000

tcatctgcag cctcatcact gtggtcatcg cctttatcca gtgcattgac tgggtctgtg     3060

tgcgctttgc atatctcaga caccatcccc agtacaggga caggactata gctgagcttc     3120

ttagaattct ttaattatga aatttactgt gacttttctg ctgattattt gcaccctaga     3180

tatc                                                                  3184


<210>  3
<211>  2699
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence for insertion of Y477A mutation, TAYT deletion, and RDG 
       peptide A20 into the fibre region

<400>  3
ctggagcctt gggttttgat tcacaaggca atatgcaact taatgtagca ggaggactaa       60

ggattgattc tcaaaacaga cgccttatac ttgatgttag ttatccgttt gatgctcaaa      120

accaactaaa tctaagacta ggacagggcc ctctttttat aaactcagcc cacaacttgg      180

atattaacta caacaaaggc ctttacgata tcttgtttac agcttcaaac aattccaaaa      240

agcttgaggt taacctaagc actgccaagg ggttgatgtt tgacgctaca gccatagcca      300

ttaatgcagg agatgggctt gaatttggtt cacctaatgc accaaacaca aatcccctca      360

aaacaaaaat tggccatggc ctagaatttg attcaaacaa ggctatggtt cctaaactag      420

gaactggcct tagttttgac agcacaggtg ccattacagt aggaaacaaa aataatgata      480

agctaacttt gtggaccaca ccagctccat ctcctaactg tagactaaat gcagagaaag      540

atgctaaact cactttggtc ttaacaaaat gtggcagtca aatacttgct acagtttcag      600

ttttggctgt taaaggcagt ttggctccaa tatctggaac agttcaaagt gctcatctta      660

ttataagatt tgacgaaaat ggagtgctac taaacaattc cttcctggac ccagaagctt      720

ggaactttag aaatggagat cttactgaag gcaacgctgt tggatttatg cctaacctat      780

cagcttatcc aaaatctcac ggtaaaactg ccaaaagtaa cattgtcagt caagtttact      840

taaacggaga caaaactaaa cctgtaacac taaccattac actaaacggt acacaggaaa      900

caggagacac aactaacgca gtacctaact tgagaggaga tctacaggtg ttggcacaga      960

aggtcgcacg tactccaagt gcatactcta tgtcattttc atgggactgg tctggccaca     1020

actacattaa tgaaatattt gccacatcct cttacacttt ttcatacatt gcccaagaat     1080

aaatttaaat atacctgtga cggaagatca cttcgcagaa taaataaatc ctggtgtccc     1140

tgttgatacc gggaagccct gggccaactt ttggcgaaaa tgagacgttg atcggcacgt     1200

aagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt     1260

atcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac     1320

cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca     1380

atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa     1440

aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca     1500

tccggagttc cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc     1560

ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca     1620

cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa     1680

cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg     1740

ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt     1800

tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca     1860

ggttcatcat gccgtctgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca     1920

gtactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtggcctta     1980

aacgcctatt taaatagaat cgtttgtgtt atgtttcaac gtgtttattt ttcaattgca     2040

gaaaatttca agtcattttt cattcagtag tatagcccca ccaccacata gcttatacag     2100

atcaccgtac cttaatcaaa ctcacagaac cctagtattc aacctgccac ctccctccca     2160

acacacagag tacacagtcc tttctccccg gctggcctta aaaagcatca tatcatgggt     2220

aacagacata ttcttaggtg ttatattcca cacggtttcc tgtcgagcca aacgctcatc     2280

agtgatatta ataaactccc cgggcagctc acttaagttc atgtcgctgt ccagctgctg     2340

agccacaggc tgctgtccaa cttgcggttg cttaacgggc ggcgaaggag aagtccacgc     2400

ctacatgggg gtagagtcat aatcgtgcat caggataggg cggtggtgct gcagcagcgc     2460

gcgaataaac tgctgccgcc gccgctccgt cctgcaggaa gatatctaca acatggcagt     2520

ggtctcctca gcgatgattc gcaccgcccg cagcataagg cgccttgtcc tccgggcaca     2580

gcagcgcacc ctgatctcac ttaaatcagc acagtaactg cagcacagca ccacaatatt     2640

gttcaaaatc ccacagtgca aggcgctgta tccaaagctc atggcgggga ccacagaac      2699


<210>  4
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of A20FMDV2 peptide

<400>  4
aacgcagtac ctaacttgag aggagatcta caggtgttgg cacagaaggt cgcacgtact       60



<210>  5
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>   A20

<400>  5

Asn Ala Val Pro Asn Leu Arg Gly Asp Leu Gln Val Leu Ala Gln Lys 
1               5                   10                  15      


Val Ala Arg Thr 
            20  


<210>  6
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E3 sequencing primer

<400>  6
gggttggggt tattctct                                                     18


<210>  7
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fibre region sequencing primer

<400>  7
gacagcacag gtgccattac a                                                 21




