                         SEQUENCE LISTING

<110>  Autolus Limited
 
<120>  METHOD

<130>  P120118PCT1

<140>  PCT/GB2021/050863
<141>  2021-04-08

<150>  GB 2005216.3
<151>  2020-04-08

<160>  34    

<170>  PatentIn version 3.5

<210>  1
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heavy chain variable region (VH) complementarity determining 
       region (CDR), CDR1

<400>  1

Gly Tyr Ala Phe Ser Ser Ser 
1               5           


<210>  2
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH CDR2

<400>  2

Tyr Pro Gly Asp Glu Asp 
1               5       


<210>  3
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH CDR3

<400>  3

Ser Leu Leu Tyr Gly Asp Tyr Leu Asp Tyr 
1               5                   10  


<210>  4
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  light chain variable region (VL) CDR, CDR1

<400>  4

Ser Ala Ser Ser Ser Val Ser Tyr Met His 
1               5                   10  


<210>  5
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL CDR2

<400>  5

Asp Thr Ser Lys Leu Ala Ser 
1               5           


<210>  6
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL CDR3

<400>  6

Gln Gln Trp Asn Ile Asn Pro Leu Thr 
1               5                   


<210>  7
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAT19 VH sequence

<400>  7

Gln Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ala Phe Ser Ser Ser 
            20                  25                  30          


Trp Met Asn Trp Val Lys Gln Arg Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Arg Ile Tyr Pro Gly Asp Glu Asp Thr Asn Tyr Ser Gly Lys Phe 
    50                  55                  60                  


Lys Asp Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Thr Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Ser Leu Leu Tyr Gly Asp Tyr Leu Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Thr Leu Thr Val Ser Ser 
        115                 


<210>  8
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAT19 VL sequence

<400>  8

Gln Ile Val Leu Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met 
            20                  25                  30          


His Trp Tyr Gln Gln Lys Ser Gly Thr Ser Pro Lys Arg Trp Ile Tyr 
        35                  40                  45              


Asp Thr Ser Lys Leu Ala Ser Gly Val Pro Asp Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Gly Thr Ser Tyr Phe Leu Thr Ile Asn Asn Met Glu Ala Glu 
65                  70                  75                  80  


Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Asn Ile Asn Pro Leu Thr 
                85                  90                  95      


Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys Arg 
            100                 105         


<210>  9
<211>  241
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAT19 VH-VL single-chain variable fragment (scFv) sequence

<400>  9

Gln Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ala Phe Ser Ser Ser 
            20                  25                  30          


Trp Met Asn Trp Val Lys Gln Arg Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Arg Ile Tyr Pro Gly Asp Glu Asp Thr Asn Tyr Ser Gly Lys Phe 
    50                  55                  60                  


Lys Asp Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Thr Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Ser Leu Leu Tyr Gly Asp Tyr Leu Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Thr Leu Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             


Ser Gly Gly Gly Gly Ser Gln Ile Val Leu Thr Gln Ser Pro Ala Ile 
    130                 135                 140                 


Met Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Ser Ala Ser 
145                 150                 155                 160 


Ser Ser Val Ser Tyr Met His Trp Tyr Gln Gln Lys Ser Gly Thr Ser 
                165                 170                 175     


Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys Leu Ala Ser Gly Val Pro 
            180                 185                 190         


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Phe Leu Thr Ile 
        195                 200                 205             


Asn Asn Met Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp 
    210                 215                 220                 


Asn Ile Asn Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
225                 230                 235                 240 


Arg 
    


<210>  10
<211>  488
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAT19 CAR with CD8 stk and 41BB/CD3z endodomain

<400>  10

Met Gly Thr Ser Leu Leu Cys Trp Met Ala Leu Cys Leu Leu Gly Ala 
1               5                   10                  15      


Asp His Ala Asp Ala Gln Val Gln Leu Gln Gln Ser Gly Pro Glu Leu 
            20                  25                  30          


Val Lys Pro Gly Ala Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr 
        35                  40                  45              


Ala Phe Ser Ser Ser Trp Met Asn Trp Val Lys Gln Arg Pro Gly Lys 
    50                  55                  60                  


Gly Leu Glu Trp Ile Gly Arg Ile Tyr Pro Gly Asp Glu Asp Thr Asn 
65                  70                  75                  80  


Tyr Ser Gly Lys Phe Lys Asp Lys Ala Thr Leu Thr Ala Asp Lys Ser 
                85                  90                  95      


Ser Thr Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser 
            100                 105                 110         


Ala Val Tyr Phe Cys Ala Arg Ser Leu Leu Tyr Gly Asp Tyr Leu Asp 
        115                 120                 125             


Tyr Trp Gly Gln Gly Thr Thr Leu Thr Val Ser Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Ile Val Leu Thr 
145                 150                 155                 160 


Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly Glu Lys Val Thr Met 
                165                 170                 175     


Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met His Trp Tyr Gln Gln 
            180                 185                 190         


Lys Ser Gly Thr Ser Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys Leu 
        195                 200                 205             


Ala Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser 
    210                 215                 220                 


Tyr Phe Leu Thr Ile Asn Asn Met Glu Ala Glu Asp Ala Ala Thr Tyr 
225                 230                 235                 240 


Tyr Cys Gln Gln Trp Asn Ile Asn Pro Leu Thr Phe Gly Ala Gly Thr 
                245                 250                 255     


Lys Leu Glu Leu Lys Arg Ser Asp Pro Thr Thr Thr Pro Ala Pro Arg 
            260                 265                 270         


Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg 
        275                 280                 285             


Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly 
    290                 295                 300                 


Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr 
305                 310                 315                 320 


Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Lys Arg 
                325                 330                 335     


Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro 
            340                 345                 350         


Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu 
        355                 360                 365             


Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala 
    370                 375                 380                 


Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu 
385                 390                 395                 400 


Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly 
                405                 410                 415     


Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu 
            420                 425                 430         


Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser 
        435                 440                 445             


Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly 
    450                 455                 460                 


Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu 
465                 470                 475                 480 


His Met Gln Ala Leu Pro Pro Arg 
                485             


<210>  11
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD22-binding domain VH CDR1

<400>  11

Asn Phe Ala Met Ala 
1               5   


<210>  12
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD22-binding domain VH CDR2

<400>  12

Ser Ile Ser Thr Gly Gly Gly Asn Thr Tyr Tyr Arg Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  13
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD22-binding domain VH CDR3

<400>  13

Gln Arg Asn Tyr Tyr Asp Gly Ser Tyr Asp Tyr Glu Gly Tyr Thr Met 
1               5                   10                  15      


Asp Ala 
        


<210>  14
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD22-binding domain VL CDR1

<400>  14

Arg Ser Ser Gln Asp Ile Gly Asn Tyr Leu Thr 
1               5                   10      


<210>  15
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD22-binding domain VL CDR2

<400>  15

Gly Ala Ile Lys Leu Glu Asp 
1               5           


<210>  16
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD22-binding domain VL CDR3

<400>  16

Leu Gln Ser Ile Gln Tyr Pro 
1               5           


<210>  17
<211>  127
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  9A8 VH sequence

<400>  17

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asn Phe 
            20                  25                  30          


Ala Met Ala Trp Val Arg Gln Pro Pro Thr Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Ser Ile Ser Thr Gly Gly Gly Asn Thr Tyr Tyr Arg Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ala Lys Asn Thr Gln Tyr 
65                  70                  75                  80  


Leu Gln Met Asp Ser Leu Arg Ser Glu Asp Thr Ala Thr Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gln Arg Asn Tyr Tyr Asp Gly Ser Tyr Asp Tyr Glu Gly Tyr 
            100                 105                 110         


Thr Met Asp Ala Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser 
        115                 120                 125         


<210>  18
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  9A8 VL sequence

<400>  18

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ser Ser Gln Asp Ile Gly Asn Tyr 
            20                  25                  30          


Leu Thr Trp Phe Gln Gln Lys Val Gly Arg Ser Pro Arg Arg Met Ile 
        35                  40                  45              


Tyr Gly Ala Ile Lys Leu Glu Asp Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Arg Ser Gly Ser Asp Tyr Ser Leu Thr Ile Ser Ser Leu Glu Ser 
65                  70                  75                  80  


Glu Asp Val Ala Asp Tyr Gln Cys Leu Gln Ser Ile Gln Tyr Pro Phe 
                85                  90                  95      


Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile Lys 
            100                 105         


<210>  19
<211>  250
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  9A8 scFV sequence

<400>  19

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ser Ser Gln Asp Ile Gly Asn Tyr 
            20                  25                  30          


Leu Thr Trp Phe Gln Gln Lys Val Gly Arg Ser Pro Arg Arg Met Ile 
        35                  40                  45              


Tyr Gly Ala Ile Lys Leu Glu Asp Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Arg Ser Gly Ser Asp Tyr Ser Leu Thr Ile Ser Ser Leu Glu Ser 
65                  70                  75                  80  


Glu Asp Val Ala Asp Tyr Gln Cys Leu Gln Ser Ile Gln Tyr Pro Phe 
                85                  90                  95      


Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile Lys Arg Ser Gly Gly Gly 
            100                 105                 110         


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Gln Leu 
        115                 120                 125             


Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Arg Ser Leu Lys Leu 
    130                 135                 140                 


Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asn Phe Ala Met Ala Trp 
145                 150                 155                 160 


Val Arg Gln Pro Pro Thr Lys Gly Leu Glu Trp Val Ala Ser Ile Ser 
                165                 170                 175     


Thr Gly Gly Gly Asn Thr Tyr Tyr Arg Asp Ser Val Lys Gly Arg Phe 
            180                 185                 190         


Thr Ile Ser Arg Asp Asp Ala Lys Asn Thr Gln Tyr Leu Gln Met Asp 
        195                 200                 205             


Ser Leu Arg Ser Glu Asp Thr Ala Thr Tyr Tyr Cys Ala Arg Gln Arg 
    210                 215                 220                 


Asn Tyr Tyr Asp Gly Ser Tyr Asp Tyr Glu Gly Tyr Thr Met Asp Ala 
225                 230                 235                 240 


Trp Gly Gln Gly Thr Ser Val Thr Val Ser 
                245                 250 


<210>  20
<211>  496
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  9A8 chimeric antigen receptor (CAR) sequence

<400>  20

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Leu Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser 
            20                  25                  30          


Ala Ser Leu Gly Asp Arg Val Thr Ile Thr Cys Arg Ser Ser Gln Asp 
        35                  40                  45              


Ile Gly Asn Tyr Leu Thr Trp Phe Gln Gln Lys Val Gly Arg Ser Pro 
    50                  55                  60                  


Arg Arg Met Ile Tyr Gly Ala Ile Lys Leu Glu Asp Gly Val Pro Ser 
65                  70                  75                  80  


Arg Phe Ser Gly Ser Arg Ser Gly Ser Asp Tyr Ser Leu Thr Ile Ser 
                85                  90                  95      


Ser Leu Glu Ser Glu Asp Val Ala Asp Tyr Gln Cys Leu Gln Ser Ile 
            100                 105                 110         


Gln Tyr Pro Phe Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile Lys Arg 
        115                 120                 125             


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Arg 
145                 150                 155                 160 


Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asn Phe 
                165                 170                 175     


Ala Met Ala Trp Val Arg Gln Pro Pro Thr Lys Gly Leu Glu Trp Val 
            180                 185                 190         


Ala Ser Ile Ser Thr Gly Gly Gly Asn Thr Tyr Tyr Arg Asp Ser Val 
        195                 200                 205             


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ala Lys Asn Thr Gln Tyr 
    210                 215                 220                 


Leu Gln Met Asp Ser Leu Arg Ser Glu Asp Thr Ala Thr Tyr Tyr Cys 
225                 230                 235                 240 


Ala Arg Gln Arg Asn Tyr Tyr Asp Gly Ser Tyr Asp Tyr Glu Gly Tyr 
                245                 250                 255     


Thr Met Asp Ala Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Asp 
            260                 265                 270         


Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile 
        275                 280                 285             


Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala 
    290                 295                 300                 


Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr 
305                 310                 315                 320 


Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu 
                325                 330                 335     


Val Ile Thr Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile 
            340                 345                 350         


Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp 
        355                 360                 365             


Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
    370                 375                 380                 


Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
385                 390                 395                 400 


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
                405                 410                 415     


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
            420                 425                 430         


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
        435                 440                 445             


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
    450                 455                 460                 


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
465                 470                 475                 480 


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                485                 490                 495     


<210>  21
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BCMA-binding domain VH CDR1

<400>  21

Gly Phe Ile Phe Ser Asp Tyr Asn 
1               5               


<210>  22
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BCMA-binding domain VH CDR2

<400>  22

Ile Ile Tyr Asp Gly Ser Ser Thr 
1               5               


<210>  23
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BCMA-binding domain VH CDR3

<400>  23

Ala Thr Arg Pro Gly Pro Phe Ala Tyr 
1               5                   


<210>  24
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BCMA-binding domain VL CDR1

<400>  24

Gln Ser Leu Leu His Ser Asn Gly Asn Thr Tyr 
1               5                   10      


<210>  25
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BCMA-binding domain VL CDR2

<400>  25

Leu Val Ser 
1           


<210>  26
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BCMA-binding domain VL CDR3

<400>  26

Val His Gly Thr His Ala Trp Thr 
1               5               


<210>  27
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR D8 VH sequence

<400>  27

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Ile Phe Ser Asp Tyr 
            20                  25                  30          


Asn Met Ala Trp Val Arg Gln Ala Pro Lys Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Thr Ile Ile Tyr Asp Gly Ser Ser Thr Asn His Gly Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Ser Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asp Ser Leu Arg Ser Glu Asp Thr Ala Thr Tyr Tyr Cys 
                85                  90                  95      


Ala Thr Arg Pro Gly Pro Phe Ala Tyr Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser 
        115 


<210>  28
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR D8 VL sequence

<400>  28

Asp Val Val Leu Thr Gln Thr Pro Pro Thr Leu Ser Ala Thr Ile Gly 
1               5                   10                  15      


Gln Ser Val Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Leu His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu His Trp Leu Leu Gln Arg Pro Gly Gln Ser 
        35                  40                  45              


Pro Gln Phe Leu Ile Tyr Leu Val Ser Gly Leu Gly Ser Gly Val Pro 
    50                  55                  60                  


Asn Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Gly Val Glu Ala Glu Asp Leu Gly Ile Tyr Tyr Cys Val His Gly 
                85                  90                  95      


Thr His Ala Trp Thr Val Gly Gly Gly Thr Lys Leu Glu Leu Lys 
            100                 105                 110     


<210>  29
<211>  692
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  D8 FabCAR sequence

<400>  29

Met Glu Thr Asp Thr Leu Ile Leu Trp Val Leu Leu Leu Leu Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Val Val Leu Thr Gln Thr Pro Pro Thr Leu Ser 
            20                  25                  30          


Ala Thr Ile Gly Gln Ser Val Ser Ile Ser Cys Arg Ser Ser Gln Ser 
        35                  40                  45              


Leu Leu His Ser Asn Gly Asn Thr Tyr Leu His Trp Leu Leu Gln Arg 
    50                  55                  60                  


Pro Gly Gln Ser Pro Gln Phe Leu Ile Tyr Leu Val Ser Gly Leu Gly 
65                  70                  75                  80  


Ser Gly Val Pro Asn Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe 
                85                  90                  95      


Thr Leu Lys Ile Ser Gly Val Glu Ala Glu Asp Leu Gly Ile Tyr Tyr 
            100                 105                 110         


Cys Val His Gly Thr His Ala Trp Thr Val Gly Gly Gly Thr Lys Leu 
        115                 120                 125             


Glu Leu Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro 
    130                 135                 140                 


Ser Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu 
145                 150                 155                 160 


Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn 
                165                 170                 175     


Ala Leu Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser 
            180                 185                 190         


Lys Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala 
        195                 200                 205             


Asp Tyr Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly 
    210                 215                 220                 


Leu Ser Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys Arg Ala 
225                 230                 235                 240 


Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
                245                 250                 255     


Pro Gly Pro Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr 
            260                 265                 270         


Ala Thr Gly Val His Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly 
        275                 280                 285             


Leu Val Gln Pro Gly Arg Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly 
    290                 295                 300                 


Phe Ile Phe Ser Asp Tyr Asn Met Ala Trp Val Arg Gln Ala Pro Lys 
305                 310                 315                 320 


Lys Gly Leu Glu Trp Val Ala Thr Ile Ile Tyr Asp Gly Ser Ser Thr 
                325                 330                 335     


Asn His Gly Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn 
            340                 345                 350         


Ala Lys Ser Thr Leu Tyr Leu Gln Met Asp Ser Leu Arg Ser Glu Asp 
        355                 360                 365             


Thr Ala Thr Tyr Tyr Cys Ala Thr Arg Pro Gly Pro Phe Ala Tyr Trp 
    370                 375                 380                 


Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro 
385                 390                 395                 400 


Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr 
                405                 410                 415     


Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr 
            420                 425                 430         


Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro 
        435                 440                 445             


Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr 
    450                 455                 460                 


Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn 
465                 470                 475                 480 


His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser 
                485                 490                 495     


Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Lys Asp Pro Lys Phe 
            500                 505                 510         


Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu 
        515                 520                 525             


Val Thr Val Ala Phe Ile Ile Phe Trp Val Lys Arg Gly Arg Lys Lys 
    530                 535                 540                 


Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr 
545                 550                 555                 560 


Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly 
                565                 570                 575     


Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala 
            580                 585                 590         


Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg 
        595                 600                 605             


Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu 
    610                 615                 620                 


Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn 
625                 630                 635                 640 


Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met 
                645                 650                 655     


Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly 
            660                 665                 670         


Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala 
        675                 680                 685             


Leu Pro Pro Arg 
    690         


<210>  30
<211>  521
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  calcineurin A alpha isoform

<400>  30

Met Ser Glu Pro Lys Ala Ile Asp Pro Lys Leu Ser Thr Thr Asp Arg 
1               5                   10                  15      


Val Val Lys Ala Val Pro Phe Pro Pro Ser His Arg Leu Thr Ala Lys 
            20                  25                  30          


Glu Val Phe Asp Asn Asp Gly Lys Pro Arg Val Asp Ile Leu Lys Ala 
        35                  40                  45              


His Leu Met Lys Glu Gly Arg Leu Glu Glu Ser Val Ala Leu Arg Ile 
    50                  55                  60                  


Ile Thr Glu Gly Ala Ser Ile Leu Arg Gln Glu Lys Asn Leu Leu Asp 
65                  70                  75                  80  


Ile Asp Ala Pro Val Thr Val Cys Gly Asp Ile His Gly Gln Phe Phe 
                85                  90                  95      


Asp Leu Met Lys Leu Phe Glu Val Gly Gly Ser Pro Ala Asn Thr Arg 
            100                 105                 110         


Tyr Leu Phe Leu Gly Asp Tyr Val Asp Arg Gly Tyr Phe Ser Ile Glu 
        115                 120                 125             


Cys Val Leu Tyr Leu Trp Ala Leu Lys Ile Leu Tyr Pro Lys Thr Leu 
    130                 135                 140                 


Phe Leu Leu Arg Gly Asn His Glu Cys Arg His Leu Thr Glu Tyr Phe 
145                 150                 155                 160 


Thr Phe Lys Gln Glu Cys Lys Ile Lys Tyr Ser Glu Arg Val Tyr Asp 
                165                 170                 175     


Ala Cys Met Asp Ala Phe Asp Cys Leu Pro Leu Ala Ala Leu Met Asn 
            180                 185                 190         


Gln Gln Phe Leu Cys Val His Gly Gly Leu Ser Pro Glu Ile Asn Thr 
        195                 200                 205             


Leu Asp Asp Ile Arg Lys Leu Asp Arg Phe Lys Glu Pro Pro Ala Tyr 
    210                 215                 220                 


Gly Pro Met Cys Asp Ile Leu Trp Ser Asp Pro Leu Glu Asp Phe Gly 
225                 230                 235                 240 


Asn Glu Lys Thr Gln Glu His Phe Thr His Asn Thr Val Arg Gly Cys 
                245                 250                 255     


Ser Tyr Phe Tyr Ser Tyr Pro Ala Val Cys Glu Phe Leu Gln His Asn 
            260                 265                 270         


Asn Leu Leu Ser Ile Leu Arg Ala His Glu Ala Gln Asp Ala Gly Tyr 
        275                 280                 285             


Arg Met Tyr Arg Lys Ser Gln Thr Thr Gly Phe Pro Ser Leu Ile Thr 
    290                 295                 300                 


Ile Phe Ser Ala Pro Asn Tyr Leu Asp Val Tyr Asn Asn Lys Ala Ala 
305                 310                 315                 320 


Val Leu Lys Tyr Glu Asn Asn Val Met Asn Ile Arg Gln Phe Asn Cys 
                325                 330                 335     


Ser Pro His Pro Tyr Trp Leu Pro Asn Phe Met Asp Val Phe Thr Trp 
            340                 345                 350         


Ser Leu Pro Phe Val Gly Glu Lys Val Thr Glu Met Leu Val Asn Val 
        355                 360                 365             


Leu Asn Ile Cys Ser Asp Asp Glu Leu Gly Ser Glu Glu Asp Gly Phe 
    370                 375                 380                 


Asp Gly Ala Thr Ala Ala Ala Arg Lys Glu Val Ile Arg Asn Lys Ile 
385                 390                 395                 400 


Arg Ala Ile Gly Lys Met Ala Arg Val Phe Ser Val Leu Arg Glu Glu 
                405                 410                 415     


Ser Glu Ser Val Leu Thr Leu Lys Gly Leu Thr Pro Thr Gly Met Leu 
            420                 425                 430         


Pro Ser Gly Val Leu Ser Gly Gly Lys Gln Thr Leu Gln Ser Ala Thr 
        435                 440                 445             


Val Glu Ala Ile Glu Ala Asp Glu Ala Ile Lys Gly Phe Ser Pro Gln 
    450                 455                 460                 


His Lys Ile Thr Ser Phe Glu Glu Ala Lys Gly Leu Asp Arg Ile Asn 
465                 470                 475                 480 


Glu Arg Met Pro Pro Arg Arg Asp Ala Met Pro Ser Asp Ala Asn Leu 
                485                 490                 495     


Asn Ser Ile Asn Lys Ala Leu Thr Ser Glu Thr Asn Gly Thr Asp Ser 
            500                 505                 510         


Asn Gly Ser Asn Ser Ser Asn Ile Gln 
        515                 520     


<210>  31
<211>  170
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  calcineurin B, type 1

<400>  31

Met Gly Asn Glu Ala Ser Tyr Pro Leu Glu Met Cys Ser His Phe Asp 
1               5                   10                  15      


Ala Asp Glu Ile Lys Arg Leu Gly Lys Arg Phe Lys Lys Leu Asp Leu 
            20                  25                  30          


Asp Asn Ser Gly Ser Leu Ser Val Glu Glu Phe Met Ser Leu Pro Glu 
        35                  40                  45              


Leu Gln Gln Asn Pro Leu Val Gln Arg Val Ile Asp Ile Phe Asp Thr 
    50                  55                  60                  


Asp Gly Asn Gly Glu Val Asp Phe Lys Glu Phe Ile Glu Gly Val Ser 
65                  70                  75                  80  


Gln Phe Ser Val Lys Gly Asp Lys Glu Gln Lys Leu Arg Phe Ala Phe 
                85                  90                  95      


Arg Ile Tyr Asp Met Asp Lys Asp Gly Tyr Ile Ser Asn Gly Glu Leu 
            100                 105                 110         


Phe Gln Val Leu Lys Met Met Val Gly Asn Asn Leu Lys Asp Thr Gln 
        115                 120                 125             


Leu Gln Gln Ile Val Asp Lys Thr Ile Ile Asn Ala Asp Lys Asp Gly 
    130                 135                 140                 


Asp Gly Arg Ile Ser Phe Glu Glu Phe Cys Ala Val Val Gly Gly Leu 
145                 150                 155                 160 


Asp Ile His Lys Lys Met Val Val Asp Val 
                165                 170 


<210>  32
<211>  172
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant calcineurin B, CnB30

<400>  32

Met Gly Asn Glu Ala Ser Tyr Pro Leu Glu Met Cys Ser His Phe Asp 
1               5                   10                  15      


Ala Asp Glu Ile Lys Arg Leu Gly Lys Arg Phe Lys Lys Leu Asp Leu 
            20                  25                  30          


Asp Asn Ser Gly Ser Leu Ser Val Glu Glu Phe Met Ser Leu Pro Glu 
        35                  40                  45              


Leu Gln Gln Asn Pro Leu Val Gln Arg Val Ile Asp Ile Phe Asp Thr 
    50                  55                  60                  


Asp Gly Asn Gly Glu Val Asp Phe Lys Glu Phe Ile Glu Gly Val Ser 
65                  70                  75                  80  


Gln Phe Ser Val Lys Gly Asp Lys Glu Gln Lys Leu Arg Phe Ala Phe 
                85                  90                  95      


Arg Ile Tyr Asp Met Asp Lys Asp Gly Tyr Ile Ser Asn Gly Glu Leu 
            100                 105                 110         


Phe Gln Val Leu Lys Met Met Val Gly Asn Asn Thr Lys Leu Ala Asp 
        115                 120                 125             


Thr Gln Leu Gln Gln Ile Val Asp Lys Thr Ile Ile Asn Ala Asp Lys 
    130                 135                 140                 


Asp Gly Asp Gly Arg Ile Ser Phe Glu Glu Phe Cys Ala Val Val Gly 
145                 150                 155                 160 


Gly Leu Asp Ile His Lys Lys Met Val Val Asp Val 
                165                 170         


<210>  33
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  basic amino acid furin target sequence


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa may be Arg or Lys

<400>  33

Arg Xaa Xaa Arg 
1               


<210>  34
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  consensus Tobacco Etch Virus (TEV) cleavage site

<400>  34

Glu Asn Leu Tyr Phe Gln Ser 
1               5           


