                         SEQUENCE LISTING

<110>  Mellizyme Biotechnology Limited
 
<120>  Enzymatic Degradation of Plastics

<130>  P33396WO1

<150>  GB2005073.8
<151>  2020-04-06

<160>  2     

<170>  PatentIn version 3.5

<210>  1
<211>  721
<212>  PRT
<213>  Ralstonia pickettii

<400>  1

Met Thr Thr Glu Ala Lys Cys Pro Phe Ser Gly His Ala Pro Ala Ala 
1               5                   10                  15      


Ser His Ala Phe Gly Gly Gly Thr Ala Asn Lys Asp Trp Trp Pro Asn 
            20                  25                  30          


Gln Leu Arg Val Asp Leu Leu Asn Gln His Ser Glu Lys Ser Asp Pro 
        35                  40                  45              


Leu Gly Ser Asn Phe Asn Tyr Arg Lys Ser Phe Asn Ala Ile Asp Tyr 
    50                  55                  60                  


Asp Ala Leu Lys Ala Asp Leu Arg Arg Leu Met Thr Asp Ser Gln Asp 
65                  70                  75                  80  


Trp Trp Pro Ala Asp Phe Gly His Tyr Gly Pro Gln Phe Ile Arg Met 
                85                  90                  95      


Ala Trp His Ala Ala Gly Thr Tyr Arg Thr Gly Asp Gly Arg Gly Gly 
            100                 105                 110         


Ala Gly Arg Gly Gln Gln Arg Phe Ala Pro Leu Asn Ser Trp Pro Asp 
        115                 120                 125             


Asn Val Asn Ile Asp Lys Ser Arg Arg Leu Leu Trp Pro Ile Lys Gln 
    130                 135                 140                 


Lys Tyr Gly Gln Ala Ile Ser Trp Ala Asp Leu Leu Ile Leu Thr Gly 
145                 150                 155                 160 


Asn Val Ala Leu Glu Thr Met Gly Phe Arg Thr Phe Gly Phe Ala Ala 
                165                 170                 175     


Gly Arg Glu Asp Thr Trp Glu Pro Asp Asn Asp Val Tyr Trp Gly Asn 
            180                 185                 190         


Glu Thr Lys Trp Leu Glu Ala Thr Arg Tyr Ser Gly Glu Arg Asn Leu 
        195                 200                 205             


Ala Asn Pro Leu Ala Ala Val Gln Met Gly Leu Ile Tyr Val Asn Pro 
    210                 215                 220                 


Glu Gly Pro Glu His Ala His Gly Asp Pro Leu Ala Ala Ala Lys Asp 
225                 230                 235                 240 


Ile Arg Glu Thr Phe Ala Arg Met Ala Met Asp Asp Glu Glu Thr Val 
                245                 250                 255     


Ala Leu Ile Ala Gly Gly His Thr Phe Gly Lys Thr His Gly Ala Gly 
            260                 265                 270         


Pro Ala Ser His Val Gly Ala Asp Val Glu Ala Ala Pro Leu Glu Ala 
        275                 280                 285             


Gln Gly Leu Gly Trp Ala Ser Thr Phe Gly Thr Gly Lys Gly Ala Asp 
    290                 295                 300                 


Ala Ile Thr Ser Gly Leu Glu Val Thr Trp Thr Gln Thr Pro Ala Gln 
305                 310                 315                 320 


Trp Ser Asn Phe Phe Phe Glu Asn Leu Phe Lys Tyr Glu Trp Val Gln 
                325                 330                 335     


Glu Lys Ser Pro Ala Gly Ala Leu Gln Trp Val Ala Lys Asp Ala Glu 
            340                 345                 350         


Ala Ile Ile Pro Gly Pro Thr Pro Asp Ser Pro Lys Arg Arg Pro Thr 
        355                 360                 365             


Met Leu Thr Thr Asp Leu Ser Leu Arg Phe Asp Pro Ala Tyr Glu Lys 
    370                 375                 380                 


Ile Ser Arg Arg Phe Leu Asp Asn Pro Gln Ala Phe Ala Glu Ala Phe 
385                 390                 395                 400 


Ala Arg Ala Trp Phe Lys Leu Thr His Arg Asp Leu Gly Pro Lys Ser 
                405                 410                 415     


Arg Tyr Leu Gly Pro Glu Val Pro Arg Glu Asp Leu Ile Trp Gln Asp 
            420                 425                 430         


Pro Leu Pro Thr Ala Thr His Lys Pro Thr Glu Ala Asp Ile Ala Asp 
        435                 440                 445             


Leu Lys Ala Lys Ile Ala Ala Ser Gly Leu Ser Ala Ser Glu Leu Val 
    450                 455                 460                 


Ala Val Ala Trp Ala Ser Ala Ser Thr Phe Arg Gly Ser Asp Lys Arg 
465                 470                 475                 480 


Gly Gly Ala Asn Gly Ala Arg Ile Arg Leu Ala Pro Gln Lys Asp Trp 
                485                 490                 495     


Ala Val Asn Gln Pro Val Ala Gly Thr Leu Ala Arg Leu Glu Glu Ile 
            500                 505                 510         


Gln Arg Ala Ser Gly Lys Ala Ser Leu Ala Asp Val Ile Val Leu Ala 
        515                 520                 525             


Gly Ser Val Gly Ile Glu Leu Ala Ala Lys Ala Ala Gly Thr Thr Ile 
    530                 535                 540                 


Thr Val Pro Phe Thr Pro Gly Arg Val Asp Ala Thr Ala Glu Gln Thr 
545                 550                 555                 560 


Asp Ala Thr Ser Phe Ser Val Leu Glu Pro Val Ala Asp Gly Phe Arg 
                565                 570                 575     


Asn Tyr Gln Lys Thr Gln Phe Ala Val Pro Gly Glu Val Leu Leu Leu 
            580                 585                 590         


Asp Arg Ala Gln Leu Leu Thr Leu Thr Ala Pro Glu Leu Thr Val Leu 
        595                 600                 605             


Ile Gly Gly Leu Arg Ala Ile Asn Ile Asn Ala Asp Gly Ser Gln His 
    610                 615                 620                 


Gly Val Phe Thr Ser Thr Pro Gly Ala Leu Thr Asn Asp Phe Phe Val 
625                 630                 635                 640 


Asn Leu Leu Asp Met Asn Thr Glu Trp Lys Pro Ala Gly Asp Ile Tyr 
                645                 650                 655     


Glu Gly Tyr Asp Arg Lys Thr Gly Glu Arg Lys Trp Thr Gly Thr Arg 
            660                 665                 670         


Val Asp Leu Val Phe Gly Ser Asn Ser Ile Leu Arg Ala Leu Ala Glu 
        675                 680                 685             


Val Phe Gly Ser Ala Asp Gly Lys Glu Arg Phe Ile Ser Glu Phe Val 
    690                 695                 700                 


Ala Ala Trp Val Lys Val Met Asn Leu Asp Arg Phe Asp Leu Ala Ala 
705                 710                 715                 720 


Ala 
    


<210>  2
<211>  431
<212>  PRT
<213>  Ralstonia pickettii

<400>  2

Met Asn Ser Leu Ile Ala Ala Leu Leu Pro Ile Gly Val Phe Gly Ser 
1               5                   10                  15      


Cys Pro Phe Met Asp Ile Gly Thr Pro Pro Ser Leu Lys Ala Glu Val 
            20                  25                  30          


Ile Ser Asp Ser Asp Phe Lys Thr Ala Leu Glu Gly Leu Asp Ile Glu 
        35                  40                  45              


Ala Leu Glu Gln Asp Leu Arg Ile Leu Met Thr Asp Ser Gln Thr Cys 
    50                  55                  60                  


Trp Pro Ala Asp Asp Gly His Tyr Gly Gly Phe Met Ile Arg Leu Ala 
65                  70                  75                  80  


Trp His Cys Ala Gly Thr Phe Arg Thr Ser Asp Gln Lys Gly Gly Cys 
                85                  90                  95      


Gly Gly Ala Gly Ile Arg Phe Pro Pro Glu Ser Asp Trp Glu Asp Asn 
            100                 105                 110         


Gly Asn Leu Asp Lys Ala Arg Ala Leu Leu Val Pro Ile Lys Gln Lys 
        115                 120                 125             


Tyr Gly Asp Ala Leu Ser Trp Gly Asp Leu Ile Ser Phe Ala Gly Thr 
    130                 135                 140                 


Val Ala Ile Arg Asp Met Gly Gly Pro Thr Asn Pro His Cys Phe Gly 
145                 150                 155                 160 


Arg Val Asp Asp Ala Asp Gly Asn Lys Ser Asp Ile Phe Gly Val Thr 
                165                 170                 175     


Asp Ser Trp Gln Asp Thr Asp Cys Val Val Gln Gly Asn Cys Gln Glu 
            180                 185                 190         


Pro Met Gly Ala Val Lys Val Gly Leu Ile Tyr Val Asn Pro Glu Gly 
        195                 200                 205             


Pro Leu Asn Asp Pro Asn Asp Leu Asn Ser Gly Gln Asn Pro Asp Pro 
    210                 215                 220                 


Glu Lys Ser Ala Val Glu Ile Arg Glu Val Phe Gly Arg Met Gly Met 
225                 230                 235                 240 


Asn Asp Ser Glu Thr Ala Ser Leu Ile Ala Gly Gly His Ala Phe Gly 
                245                 250                 255     


Lys Cys His Gly Ala Gly Val Met Thr Ser Gly Phe Glu Gly Pro Trp 
            260                 265                 270         


Thr Thr Thr Pro Ser Gln Trp Thr Asn Gln Phe Leu Thr Gly Met Leu 
        275                 280                 285             


Asp Glu Glu Trp Glu Gln Val Ala Thr Pro Ser Gly Ser Ala Val Gln 
    290                 295                 300                 


Trp Gln Thr Lys Asp Arg Thr Ser Ile Leu Ala Gly Thr Met Arg Leu 
305                 310                 315                 320 


Thr Ala Asp Leu Ala Leu Val Asn Asp Asp Ala Tyr Leu Ala Leu Ala 
                325                 330                 335     


Lys His Trp Val Cys Asp Gln Gln Lys Leu Asp Ile Ala Phe Ala Ala 
            340                 345                 350         


Ser Trp Lys Lys Leu Val Glu Ser Gly Gly Gly Trp Leu Pro Val Glu 
        355                 360                 365             


Asp Arg Arg Cys Glu Pro Glu Ser Lys Ala Thr Gly Gln Gln Thr Asp 
    370                 375                 380                 


Pro Gln Lys Asn Phe His Ser Val Cys Ala Thr Thr Thr Thr Asp Asp 
385                 390                 395                 400 


Ser Gln Glu Ala Ser Thr Ala Thr Lys Phe Cys Leu Ser Phe Leu Ala 
                405                 410                 415     


Val Val Leu Thr Pro Phe Ser Leu Gly Val His Ser Trp Leu Ala 
            420                 425                 430     


