                         SEQUENCE LISTING

<110>  Mediphage Bioceuticals, Inc.
              
 
<120>  VECTORS FOR PRODUCING VIRUS-LIKE PARTICLES AND USES THEREOF

<130>  4471.005PC02

<150>  US 63/124,397
<151>  2020-12-11

<150>  US 63/003,281
<151>  2020-03-31


<160>  104   

<170>  PatentIn version 3.5

<210>  1
<211>  222
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Membrane Protein

<400>  1

Met Ala Asp Ser Asn Gly Thr Ile Thr Val Glu Glu Leu Lys Lys Leu 
1               5                   10                  15      


Leu Glu Gln Trp Asn Leu Val Ile Gly Phe Leu Phe Leu Thr Trp Ile 
            20                  25                  30          


Cys Leu Leu Gln Phe Ala Tyr Ala Asn Arg Asn Arg Phe Leu Tyr Ile 
        35                  40                  45              


Ile Lys Leu Ile Phe Leu Trp Leu Leu Trp Pro Val Thr Leu Ala Cys 
    50                  55                  60                  


Phe Val Leu Ala Ala Val Tyr Arg Ile Asn Trp Ile Thr Gly Gly Ile 
65                  70                  75                  80  


Ala Ile Ala Met Ala Cys Leu Val Gly Leu Met Trp Leu Ser Tyr Phe 
                85                  90                  95      


Ile Ala Ser Phe Arg Leu Phe Ala Arg Thr Arg Ser Met Trp Ser Phe 
            100                 105                 110         


Asn Pro Glu Thr Asn Ile Leu Leu Asn Val Pro Leu His Gly Thr Ile 
        115                 120                 125             


Leu Thr Arg Pro Leu Leu Glu Ser Glu Leu Val Ile Gly Ala Val Ile 
    130                 135                 140                 


Leu Arg Gly His Leu Arg Ile Ala Gly His His Leu Gly Arg Cys Asp 
145                 150                 155                 160 


Ile Lys Asp Leu Pro Lys Glu Ile Thr Val Ala Thr Ser Arg Thr Leu 
                165                 170                 175     


Ser Tyr Tyr Lys Leu Gly Ala Ser Gln Arg Val Ala Gly Asp Ser Gly 
            180                 185                 190         


Phe Ala Ala Tyr Ser Arg Tyr Arg Ile Gly Asn Tyr Lys Leu Asn Thr 
        195                 200                 205             


Asp His Ser Ser Ser Ser Asp Asn Ile Ala Leu Leu Val Gln 
    210                 215                 220         


<210>  2
<211>  669
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Membrane Protein

<400>  2
atggcagatt ccaacggtac tattaccgtt gaagagctta aaaagctcct tgaacaatgg       60

aacctagtaa taggtttcct attccttaca tggatttgtc ttctacaatt tgcctatgcc      120

aacaggaata ggtttttgta tataattaag ttaattttcc tctggctgtt atggccagta      180

actttagctt gttttgtgct tgctgctgtt tacagaataa attggatcac cggtggaatt      240

gctatcgcaa tggcttgtct tgtaggcttg atgtggctca gctacttcat tgcttctttc      300

agactgtttg cgcgtacgcg ttccatgtgg tcattcaatc cagaaactaa cattcttctc      360

aacgtgccac tccatggcac tattctgacc agaccgcttc tagaaagtga actcgtaatc      420

ggagctgtga tccttcgtgg acatcttcgt attgctggac accatctagg acgctgtgac      480

atcaaggacc tgcctaaaga aatcactgtt gctacatcac gaacgctttc ttattacaaa      540

ttgggagctt cgcagcgtgt agcaggtgac tcaggttttg ctgcatacag tcgctacagg      600

attggcaact ataaattaaa cacagaccat tccagtagca gtgacaatat tgctttgctt      660

gtacagtaa                                                              669


<210>  3
<211>  75
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Envelope Protein

<400>  3

Met Tyr Ser Phe Val Ser Glu Glu Thr Gly Thr Leu Ile Val Asn Ser 
1               5                   10                  15      


Val Leu Leu Phe Leu Ala Phe Val Val Phe Leu Leu Val Thr Leu Ala 
            20                  25                  30          


Ile Leu Thr Ala Leu Arg Leu Cys Ala Tyr Cys Cys Asn Ile Val Asn 
        35                  40                  45              


Val Ser Leu Val Lys Pro Ser Phe Tyr Val Tyr Ser Arg Val Lys Asn 
    50                  55                  60                  


Leu Asn Ser Ser Arg Val Pro Asp Leu Leu Val 
65                  70                  75  


<210>  4
<211>  228
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Envelope Protein

<400>  4
atgtactcat tcgtttcgga agagacaggt acgttaatag ttaatagcgt acttcttttt       60

cttgctttcg tggtattctt gctagttaca ctagccatcc ttactgcgct tcgattgtgt      120

gcgtactgct gcaatattgt taacgtgagt cttgtaaaac cttcttttta cgtttactct      180

cgtgttaaaa atctgaattc ttctagagtt cctgatcttc tggtctaa                   228


<210>  5
<211>  1273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Spike protein

<400>  5

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  6
<211>  1118
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Spike protein

<400>  6
atgtttgttt ttcttgtttt attgccacta gtctctagtc agtgtgttaa tcttacaacc       60

agaactcaat taccccctgc atacactaat tctttcacac gtggtgttta ttaccctgac      120

aaagttttca gatcctcagt tttacattca actcaggact tgttcttacc tttcttttcc      180

aatgttactt ggttccatgc tatacatgtc tctgggacca atggtactaa gaggtttgat      240

aaccctgtcc taccatttaa tgatggtgtt tattttgctt ccactgagaa gtctaacata      300

ataagaggct ggatttttgg tactacttta gattcgaaga cccagtccct acttattgtt      360

aataacgcta ctaatgttgt tattaaagtc tgtgaatttc aattttgtaa tgatccattt      420

ttgggtgttt attaccacaa aaacaacaaa agttggatgg aaagtgagtt cagagtttat      480

tctagtgcga ataattgcac ttttgaatat gtctctcagc cttttcttat ggaccttgaa      540

ggaaaacagg gtaatttcaa aaatcttagg gaatttgtgt ttaagaatat tgatggttat      600

tttaaaatat attctaagca cacgcctatt aatttagtgc gtgatctccc tcagggtttt      660

tcggctttag aaccattggt agatttgcca ataggtatta acatcactag gtttcaaact      720

ttacttgctt tacatagaag ttatttgact cctggtgatt cttcttcagg ttggacagct      780

ggtgctgcag cttattatgt gggttatctt caacctagga cttttctatt aaaatataat      840

gaaaatggaa ccattacaga tgctgtagac tgtgcacttg accctctctc agaaacaaag      900

tgtacgttga aatccttcac tgtagaaaaa ggaatctatc aaacttctaa ctttagagtc      960

caaccaacag aatctattgt tagatttcct aatattacaa acttgtgccc ttttggtgaa     1020

gtttttaacg ccaccagatt tgcatctgtt tatgcttgga acaggaagag aatcagcaac     1080

tgtgttgctg attattctgt cctatataat tccgcatc                             1118


<210>  7
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Internal Fusion Peptide

<400>  7

Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala 
1               5                   10                  15      


Gly Phe 
        


<210>  8
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Internal Fusion Peptide

<400>  8
tcatttattg aagatctact tttcaacaaa gtgacacttg cagatgctgg cttc             54


<210>  9
<211>  254
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  receptor-binding domain

<400>  9

Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr 
1               5                   10                  15      


Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys 
            20                  25                  30          


Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe 
        35                  40                  45              


Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr 
    50                  55                  60                  


Asn Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln 
65                  70                  75                  80  


Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu 
                85                  90                  95      


Pro Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu 
            100                 105                 110         


Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg 
        115                 120                 125             


Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr 
    130                 135                 140                 


Gln Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr 
145                 150                 155                 160 


Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr 
                165                 170                 175     


Gln Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro 
            180                 185                 190         


Ala Thr Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys 
        195                 200                 205             


Cys Val Asn Phe Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr 
    210                 215                 220                 


Glu Ser Asn Lys Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile 
225                 230                 235                 240 


Ala Asp Thr Thr Asp Ala Val Arg Asp Pro Gln Thr Leu Glu 
                245                 250                 


<210>  10
<211>  762
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  receptor-binding domain

<400>  10
cctaatatta caaacttgtg cccttttggt gaagttttta acgccaccag atttgcatct       60

gtttatgctt ggaacaggaa gagaatcagc aactgtgttg ctgattattc tgtcctatat      120

aattccgcat cattttccac ttttaagtgt tatggagtgt ctcctactaa attaaatgat      180

ctctgcttta ctaatgtcta tgcagattca tttgtaatta gaggtgatga agtcagacaa      240

atcgctccag ggcaaactgg aaagattgct gattataatt ataaattacc agatgatttt      300

acaggctgcg ttatagcttg gaattctaac aatcttgatt ctaaggttgg tggtaattat      360

aattacctgt atagattgtt taggaagtct aatctcaaac cttttgagag agatatttca      420

actgaaatct atcaggccgg tagcacacct tgtaatggtg ttgaaggttt taattgttac      480

tttcctttac aatcatatgg tttccaaccc actaatggtg ttggttacca accatacaga      540

gtagtagtac tttcttttga acttctacat gcaccagcaa ctgtttgtgg acctaaaaag      600

tctactaatt tggttaaaaa caaatgtgtc aatttcaact tcaatggttt aacaggcaca      660

ggtgttctta ctgagtctaa caaaaagttt ctgcctttcc aacaatttgg cagagacatt      720

gctgacacta ctgatgctgt ccgtgatcca cagacacttg ag                         762


<210>  11
<211>  192
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  immunogenic sequence

<400>  11

Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr 
1               5                   10                  15      


Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys 
            20                  25                  30          


Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe 
        35                  40                  45              


Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr 
    50                  55                  60                  


Asn Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln 
65                  70                  75                  80  


Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu 
                85                  90                  95      


Pro Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu 
            100                 105                 110         


Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg 
        115                 120                 125             


Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr 
    130                 135                 140                 


Gln Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr 
145                 150                 155                 160 


Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr 
                165                 170                 175     


Gln Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro 
            180                 185                 190         


<210>  12
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  12

Ser Phe Ile Glu Asp Leu 
1               5       


<210>  13
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  13

Gly Val Tyr Tyr Pro 
1               5   


<210>  14
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  14

Phe Leu Pro Phe 
1               


<210>  15
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  15

Val Leu Pro Phe 
1               


<210>  16
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  16

Ser Leu Leu Ile 
1               


<210>  17
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  17

Leu Pro Ile Gly Ile 
1               5   


<210>  18
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  18

Ala Ala Tyr Tyr Val 
1               5   


<210>  19
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  19

Thr Phe Leu Leu 
1               


<210>  20
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  20

Ala Val Asp Cys 
1               


<210>  21
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  21

Ile Val Arg Phe Pro 
1               5   


<210>  22
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  22

Ile Ser Asn Cys 
1               


<210>  23
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  23

Leu Cys Phe Thr 
1               


<210>  24
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  24

Tyr Asn Tyr Lys Leu 
1               5   


<210>  25
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  25

Ile Ala Trp Asn 
1               


<210>  26
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  26

Val Val Val Leu Ser Phe 
1               5       


<210>  27
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  27

Cys Val Asn Phe 
1               


<210>  28
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  28

Gly Leu Thr Gly 
1               


<210>  29
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  29

Val Ala Val Leu Tyr 
1               5   


<210>  30
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  30

Gly Cys Leu Ile 
1               


<210>  31
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  31

Gly Ile Cys Ala 
1               


<210>  32
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  32

Phe Thr Ile Ser 
1               


<210>  33
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  33

Ser Val Asp Cys 
1               


<210>  34
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  34

Tyr Gly Ser Phe Cys 
1               5   


<210>  35
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  35

Phe Asn Phe Ser 
1               


<210>  36
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  36

Arg Asp Leu Ile Cys Ala Gln 
1               5           


<210>  37
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  37

Val Leu Pro Pro Leu Leu 
1               5       


<210>  38
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  38

Ile Pro Phe Ala 
1               


<210>  39
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  39

Tyr Arg Phe Asn 
1               


<210>  40
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  40

Lys Leu Gln Asp Val Val Asn 
1               5           


<210>  41
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  41

Gly Ala Ile Ser Ser 
1               5   


<210>  42
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  42

Glu Val Gln Ile Asp Arg Leu Ile 
1               5               


<210>  43
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  43

Tyr Val Thr Gln Gln Leu 
1               5       


<210>  44
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  44

His Leu Met Ser Phe 
1               5   


<210>  45
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  45

Gly Val Val His Leu Phe 
1               5       


<210>  46
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  46

Trp Phe Val Thr 
1               


<210>  47
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  47

Ile Asn Ala Ser 
1               


<210>  48
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  48

Leu Leu Gln Phe 
1               


<210>  49
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  49

Leu Trp Leu Leu Trp Pro 
1               5       


<210>  50
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  50

Leu Met Trp Leu 
1               


<210>  51
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  51

Ser Phe Arg Leu Phe 
1               5   


<210>  52
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  52

Phe Asn Pro Glu Thr Asn 
1               5       


<210>  53
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  53

Ile Thr Val Ala 
1               


<210>  54
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conserved Amino Acid Sequence

<400>  54

Leu Arg Leu Cys 
1               


<210>  55
<211>  245
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  recombinant spike protein

<400>  55

Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr 
1               5                   10                  15      


Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys 
            20                  25                  30          


Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe 
        35                  40                  45              


Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr 
    50                  55                  60                  


Asn Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln 
65                  70                  75                  80  


Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu 
                85                  90                  95      


Pro Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu 
            100                 105                 110         


Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg 
        115                 120                 125             


Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr 
    130                 135                 140                 


Gln Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr 
145                 150                 155                 160 


Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr 
                165                 170                 175     


Gln Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro 
            180                 185                 190         


Gly Gly Gly Gly Gly Gly Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys 
        195                 200                 205             


Val Thr Leu Ala Asp Ala Gly Phe Gly Gly Gly Gly Gly Gly Trp Pro 
    210                 215                 220                 


Trp Tyr Ile Trp Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met 
225                 230                 235                 240 


Val Thr Ile Met Leu 
                245 


<210>  56
<211>  738
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  recombinant spike protein

<400>  56
ccaaacatta ccaacctgtg ccccttcggc gaggtgttca acgccacacg gttcgccagc       60

gtgtacgcct ggaacagaaa gcggatcagc aactgcgtgg ccgactacag tgtcctgtat      120

aactccgcca gcttttctac attcaagtgc tacggcgtct cccctaccaa gctgaacgac      180

ctgtgcttca ccaatgtgta cgccgattct ttcgtgatca gaggcgacga ggtgcggcag      240

atcgcccctg gccagaccgg aaagatcgct gattacaact acaagctgcc tgatgacttc      300

accggctgcg tgatcgcctg gaactccaac aacctggaca gcaaggtggg gggcaactac      360

aactacctgt acagactgtt cagaaagagc aatctgaagc ctttcgagag agatatcagc      420

acagagatct accaggccgg cagcacccct tgtaatggcg ttgagggctt caattgctac      480

tttccactgc agagctatgg ctttcagcct acaaacggcg tgggctacca accttacaga      540

gtggtggtgc tgtctttcga gctgctgcac gcccctggcg gaggaggagg cggatctttc      600

atcgaggacc tgctgttcaa caaggtgacc ctggccgacg ccggttttgg cggtggcggc      660

ggcggctggc cttggtacat ctggctgggc ttcatcgccg gactgatcgc catcgtgatg      720

gtcaccatca tgctgtga                                                    738


<210>  57
<211>  580
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  single open reading frame for coronavirus VLP

<400>  57

Met Tyr Ser Phe Val Ser Glu Glu Thr Gly Thr Leu Ile Val Asn Ser 
1               5                   10                  15      


Val Leu Leu Phe Leu Ala Phe Val Val Phe Leu Leu Val Thr Leu Ala 
            20                  25                  30          


Ile Leu Thr Ala Leu Arg Leu Cys Ala Tyr Cys Cys Asn Ile Val Asn 
        35                  40                  45              


Val Ser Leu Val Lys Pro Ser Phe Tyr Val Tyr Ser Arg Val Lys Asn 
    50                  55                  60                  


Leu Asn Ser Ser Arg Val Pro Asp Leu Leu Val Ala Thr Asn Phe Ser 
65                  70                  75                  80  


Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro Met Ala 
                85                  90                  95      


Asp Ser Asn Gly Thr Ile Thr Val Glu Glu Leu Lys Lys Leu Leu Glu 
            100                 105                 110         


Gln Trp Asn Leu Val Ile Gly Phe Leu Phe Leu Thr Trp Ile Cys Leu 
        115                 120                 125             


Leu Gln Phe Ala Tyr Ala Asn Arg Asn Arg Phe Leu Tyr Ile Ile Lys 
    130                 135                 140                 


Leu Ile Phe Leu Trp Leu Leu Trp Pro Val Thr Leu Ala Cys Phe Val 
145                 150                 155                 160 


Leu Ala Ala Val Tyr Arg Ile Asn Trp Ile Thr Gly Gly Ile Ala Ile 
                165                 170                 175     


Ala Met Ala Cys Leu Val Gly Leu Met Trp Leu Ser Tyr Phe Ile Ala 
            180                 185                 190         


Ser Phe Arg Leu Phe Ala Arg Thr Arg Ser Met Trp Ser Phe Asn Pro 
        195                 200                 205             


Glu Thr Asn Ile Leu Leu Asn Val Pro Leu His Gly Thr Ile Leu Thr 
    210                 215                 220                 


Arg Pro Leu Leu Glu Ser Glu Leu Val Ile Gly Ala Val Ile Leu Arg 
225                 230                 235                 240 


Gly His Leu Arg Ile Ala Gly His His Leu Gly Arg Cys Asp Ile Lys 
                245                 250                 255     


Asp Leu Pro Lys Glu Ile Thr Val Ala Thr Ser Arg Thr Leu Ser Tyr 
            260                 265                 270         


Tyr Lys Leu Gly Ala Ser Gln Arg Val Ala Gly Asp Ser Gly Phe Ala 
        275                 280                 285             


Ala Tyr Ser Arg Tyr Arg Ile Gly Asn Tyr Lys Leu Asn Thr Asp His 
    290                 295                 300                 


Ser Ser Ser Ser Asp Asn Ile Ala Leu Leu Val Gln Ala Thr Asn Phe 
305                 310                 315                 320 


Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro Pro 
                325                 330                 335     


Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg 
            340                 345                 350         


Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val 
        355                 360                 365             


Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys 
    370                 375                 380                 


Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn 
385                 390                 395                 400 


Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile 
                405                 410                 415     


Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro 
            420                 425                 430         


Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp 
        435                 440                 445             


Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys 
    450                 455                 460                 


Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln 
465                 470                 475                 480 


Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe 
                485                 490                 495     


Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln 
            500                 505                 510         


Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Gly 
        515                 520                 525             


Gly Gly Gly Gly Gly Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val 
    530                 535                 540                 


Thr Leu Ala Asp Ala Gly Phe Gly Gly Gly Gly Gly Gly Trp Pro Trp 
545                 550                 555                 560 


Tyr Ile Trp Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met Val 
                565                 570                 575     


Thr Ile Met Leu 
            580 


<210>  58
<211>  1743
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  single open reading frame for coronavirus VLP

<400>  58
atgtactctt tcgtgtctga ggaaaccggc accctgatcg tgaacagcgt gctgctgttt       60

ctggccttcg tggttttcct gctggtcacc ctcgccatcc tgaccgccct gcggctgtgc      120

gcctactgct gcaacatcgt gaacgtgtct ctggtcaaac ctagcttcta cgtgtatagc      180

cgggtgaaga acctgaattc tagcagggtg cccgacctgc tggtggccac caacttcagc      240

ctgctgaaac aggctggcga tgtggaagag aaccctggac ctatggccga tagcaacggc      300

accattacag tggaggaact caaaaagctg ctggaacagt ggaatcttgt gatcggcttc      360

ctgttcctga cctggatctg cctgctgcag ttcgcctacg ccaaccgcaa cagattcctg      420

tacatcatca aactgatctt cctgtggctg ctgtggcccg tgaccctggc ttgtttcgtg      480

ctggctgctg tttatagaat caactggatc acaggcggca tcgcaatcgc catggcctgt      540

ctggtgggcc tgatgtggct gagctacttc atcgccagct ttagactgtt cgctagaaca      600

agaagcatgt ggtcctttaa ccccgagaca aacatcctcc tgaatgtgcc actgcatggc      660

accatcctga caagacccct gctggaaagc gagctggtca tcggcgccgt gatcctgcgg      720

ggccacctga gaatcgctgg ccaccacctg ggcagatgtg acatcaagga cctgcccaag      780

gaaatcactg tggccacaag cagaaccctc agctactaca agctgggagc ctctcagaga      840

gtggccggcg acagcggctt cgccgcctac agccggtacc ggattggcaa ttacaaactg      900

aacaccgacc acagctccag cagcgacaac atcgctctgc tagtgcaggc caccaatttc      960

agcctgctga agcaagctgg agatgtggaa gaaaaccccg gccctccaaa cattaccaac     1020

ctgtgcccct tcggcgaggt gttcaacgcc acacggttcg ccagcgtgta cgcctggaac     1080

agaaagcgga tcagcaactg cgtggccgac tacagtgtcc tgtataactc cgccagcttt     1140

tctacattca agtgctacgg cgtctcccct accaagctga acgacctgtg cttcaccaat     1200

gtgtacgccg attctttcgt gatcagaggc gacgaggtgc ggcagatcgc ccctggccag     1260

accggaaaga tcgctgatta caactacaag ctgcctgatg acttcaccgg ctgcgtgatc     1320

gcctggaact ccaacaacct ggacagcaag gtggggggca actacaacta cctgtacaga     1380

ctgttcagaa agagcaatct gaagcctttc gagagagata tcagcacaga gatctaccag     1440

gccggcagca ccccttgtaa tggcgttgag ggcttcaatt gctactttcc actgcagagc     1500

tatggctttc agcctacaaa cggcgtgggc taccaacctt acagagtggt ggtgctgtct     1560

ttcgagctgc tgcacgcccc tggcggagga ggaggcggat ctttcatcga ggacctgctg     1620

ttcaacaagg tgaccctggc cgacgccggt tttggcggtg gcggcggcgg ctggccttgg     1680

tacatctggc tgggcttcat cgccggactg atcgccatcg tgatggtcac catcatgctg     1740

tga                                                                   1743


<210>  59
<211>  2523
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  expression cassette for VLP

<400>  59
cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt       60

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      120

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      180

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      240

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      300

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      360

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      420

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      480

acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcca      540

ccatgtactc tttcgtgtct gaggaaaccg gcaccctgat cgtgaacagc gtgctgctgt      600

ttctggcctt cgtggttttc ctgctggtca ccctcgccat cctgaccgcc ctgcggctgt      660

gcgcctactg ctgcaacatc gtgaacgtgt ctctggtcaa acctagcttc tacgtgtata      720

gccgggtgaa gaacctgaat tctagcaggg tgcccgacct gctggtggcc accaacttca      780

gcctgctgaa acaggctggc gatgtggaag agaaccctgg acctatggcc gatagcaacg      840

gcaccattac agtggaggaa ctcaaaaagc tgctggaaca gtggaatctt gtgatcggct      900

tcctgttcct gacctggatc tgcctgctgc agttcgccta cgccaaccgc aacagattcc      960

tgtacatcat caaactgatc ttcctgtggc tgctgtggcc cgtgaccctg gcttgtttcg     1020

tgctggctgc tgtttataga atcaactgga tcacaggcgg catcgcaatc gccatggcct     1080

gtctggtggg cctgatgtgg ctgagctact tcatcgccag ctttagactg ttcgctagaa     1140

caagaagcat gtggtccttt aaccccgaga caaacatcct cctgaatgtg ccactgcatg     1200

gcaccatcct gacaagaccc ctgctggaaa gcgagctggt catcggcgcc gtgatcctgc     1260

ggggccacct gagaatcgct ggccaccacc tgggcagatg tgacatcaag gacctgccca     1320

aggaaatcac tgtggccaca agcagaaccc tcagctacta caagctggga gcctctcaga     1380

gagtggccgg cgacagcggc ttcgccgcct acagccggta ccggattggc aattacaaac     1440

tgaacaccga ccacagctcc agcagcgaca acatcgctct gctagtgcag gccaccaatt     1500

tcagcctgct gaagcaagct ggagatgtgg aagaaaaccc cggccctcca aacattacca     1560

acctgtgccc cttcggcgag gtgttcaacg ccacacggtt cgccagcgtg tacgcctgga     1620

acagaaagcg gatcagcaac tgcgtggccg actacagtgt cctgtataac tccgccagct     1680

tttctacatt caagtgctac ggcgtctccc ctaccaagct gaacgacctg tgcttcacca     1740

atgtgtacgc cgattctttc gtgatcagag gcgacgaggt gcggcagatc gcccctggcc     1800

agaccggaaa gatcgctgat tacaactaca agctgcctga tgacttcacc ggctgcgtga     1860

tcgcctggaa ctccaacaac ctggacagca aggtgggggg caactacaac tacctgtaca     1920

gactgttcag aaagagcaat ctgaagcctt tcgagagaga tatcagcaca gagatctacc     1980

aggccggcag caccccttgt aatggcgttg agggcttcaa ttgctacttt ccactgcaga     2040

gctatggctt tcagcctaca aacggcgtgg gctaccaacc ttacagagtg gtggtgctgt     2100

ctttcgagct gctgcacgcc cctggcggag gaggaggcgg atctttcatc gaggacctgc     2160

tgttcaacaa ggtgaccctg gccgacgccg gttttggcgg tggcggcggc ggctggcctt     2220

ggtacatctg gctgggcttc atcgccggac tgatcgccat cgtgatggtc accatcatgc     2280

tgtgaacggc cggctgatca taatcagcca taccacattt gtagaggttt tacttgcttt     2340

aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt     2400

taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac     2460

aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc     2520

tta                                                                   2523


<210>  60
<211>  2510
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  expression cassette for VLP

<400>  60
cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt       60

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      120

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      180

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      240

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      300

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      360

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      420

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      480

acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcca      540

ccatgtactc tttcgtgtct gaggaaaccg gcaccctgat cgtgaacagc gtgctgctgt      600

ttctggcctt cgtggttttc ctgctggtca ccctcgccat cctgaccgcc ctgcggctgt      660

gcgcctactg ctgcaacatc gtgaacgtgt ctctggtcaa acctagcttc tacgtgtata      720

gccgggtgaa gaacctgaat tctagcaggg tgcccgacct gctggtggcc accaacttca      780

gcctgctgaa acaggctggc gatgtggaag agaaccctgg acctatggcc gatagcaacg      840

gcaccattac agtggaggaa ctcaaaaagc tgctggaaca gtggaatctt gtgatcggct      900

tcctgttcct gacctggatc tgcctgctgc agttcgccta cgccaaccgc aacagattcc      960

tgtacatcat caaactgatc ttcctgtggc tgctgtggcc cgtgaccctg gcttgtttcg     1020

tgctggctgc tgtttataga atcaactgga tcacaggcgg catcgcaatc gccatggcct     1080

gtctggtggg cctgatgtgg ctgagctact tcatcgccag ctttagactg ttcgctagaa     1140

caagaagcat gtggtccttt aaccccgaga caaacatcct cctgaatgtg ccactgcatg     1200

gcaccatcct gacaagaccc ctgctggaaa gcgagctggt catcggcgcc gtgatcctgc     1260

ggggccacct gagaatcgct ggccaccacc tgggcagatg tgacatcaag gacctgccca     1320

aggaaatcac tgtggccaca agcagaaccc tcagctacta caagctggga gcctctcaga     1380

gagtggccgg cgacagcggc ttcgccgcct acagccggta ccggattggc aattacaaac     1440

tgaacaccga ccacagctcc agcagcgaca acatcgctct gctagtgcag gccaccaatt     1500

tcagcctgct gaagcaagct ggagatgtgg aagaaaaccc cggccctcca aacattacca     1560

acctgtgccc cttcggcgag gtgttcaacg ccacacggtt cgccagcgtg tacgcctgga     1620

acagaaagcg gatcagcaac tgcgtggccg actacagtgt cctgtataac tccgccagct     1680

tttctacatt caagtgctac ggcgtctccc ctaccaagct gaacgacctg tgcttcacca     1740

atgtgtacgc cgattctttc gtgatcagag gcgacgaggt gcggcagatc gcccctggcc     1800

agaccggaaa gatcgctgat tacaactaca agctgcctga tgacttcacc ggctgcgtga     1860

tcgcctggaa ctccaacaac ctggacagca aggtgggggg caactacaac tacctgtaca     1920

gactgttcag aaagagcaat ctgaagcctt tcgagagaga tatcagcaca gagatctacc     1980

aggccggcag caccccttgt aatggcgttg agggcttcaa ttgctacttt ccactgcaga     2040

gctatggctt tcagcctaca aacggcgtgg gctaccaacc ttacagagtg gtggtgctgt     2100

ctttcgagct gctgcacgcc cctggcggag gaggaggcgg atctttcatc gaggacctgc     2160

tgttcaacaa ggtgaccctg gccgacgccg gttttggcgg tggcggcggc ggctggcctt     2220

ggtacatctg gctgggcttc atcgccggac tgatcgccat cgtgatggtc accatcatgc     2280

tgtgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt     2340

gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca     2400

ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga     2460

ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg                2510


<210>  61
<211>  3273
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  expression cassette for VLP

<400>  61
cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt       60

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      120

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      180

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      240

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      300

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      360

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      420

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      480

acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcca      540

ccatgtactc tttcgtgtct gaggaaaccg gcaccctgat cgtgaacagc gtgctgctgt      600

ttctggcctt cgtggttttc ctgctggtca ccctcgccat cctgaccgcc ctgcggctgt      660

gcgcctactg ctgcaacatc gtgaacgtgt ctctggtcaa acctagcttc tacgtgtata      720

gccgggtgaa gaacctgaat tctagcaggg tgcccgacct gctggtggcc accaacttca      780

gcctgctgaa acaggctggc gatgtggaag agaaccctgg acctgccgat agcaacggca      840

ccattacagt ggaggaactc aaaaagctgc tggaacagtg gaatcttgtg atcggcttcc      900

tgttcctgac ctggatctgc ctgctgcagt tcgcctacgc caaccgcaac agattcctgt      960

acatcatcaa actgatcttc ctgtggctgc tgtggcccgt gaccctggct tgtttcgtgc     1020

tggctgctgt ttatagaatc aactggatca caggcggcat cgcaatcgcc atggcctgtc     1080

tggtgggcct gatgtggctg agctacttca tcgccagctt tagactgttc gctagaacaa     1140

gaagcatgtg gtcctttaac cccgagacaa acatcctcct gaatgtgcca ctgcatggca     1200

ccatcctgac aagacccctg ctggaaagcg agctggtcat cggcgccgtg atcctgcggg     1260

gccacctgag aatcgctggc caccacctgg gcagatgtga catcaaggac ctgcccaagg     1320

aaatcactgt ggccacaagc agaaccctca gctactacaa gctgggagcc tctcagagag     1380

tggccggcga cagcggcttc gccgcctaca gccggtaccg gattggcaat tacaaactga     1440

acaccgacca cagctccagc agcgacaaca tcgctctgct agtgcaggcc accaatttca     1500

gcctgctgaa gcaagctgga gatgtggaag aaaaccccgg ccctccaaac attaccaacc     1560

tgtgcccctt cggcgaggtg ttcaacgcca cacggttcgc cagcgtgtac gcctggaaca     1620

gaaagcggat cagcaactgc gtggccgact acagtgtcct gtataactcc gccagctttt     1680

ctacattcaa gtgctacggc gtctccccta ccaagctgaa cgacctgtgc ttcaccaatg     1740

tgtacgccga ttctttcgtg atcagaggcg acgaggtgcg gcagatcgcc cctggccaga     1800

ccggaaagat cgctgattac aactacaagc tgcctgatga cttcaccggc tgcgtgatcg     1860

cctggaactc caacaacctg gacagcaagg tggggggcaa ctacaactac ctgtacagac     1920

tgttcagaaa gagcaatctg aagcctttcg agagagatat cagcacagag atctaccagg     1980

ccggcagcac cccttgtaat ggcgttgagg gcttcaattg ctactttcca ctgcagagct     2040

atggctttca gcctacaaac ggcgtgggct accaacctta cagagtggtg gtgctgtctt     2100

tcgagctgct gcacgcccct ggcggaggag gaggcggatc tttcatcgag gacctgctgt     2160

tcaacaaggt gaccctggcc gacgccggtt ttggcggtgg cggcggcggc tggccttggt     2220

acatctggct gggcttcatc gccggactga tcgccatcgt gatggtcacc atcatgctgg     2280

agggcagggg aagtcttcta acatgcgggg acgtggagga aaatcccggc ccagagagcg     2340

acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc ctgaacggcg     2400

tggagttcga gctggtgggc ggcggagagg gcacccccga gcagggccgc atgaccaaca     2460

agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc cacgtgatgg     2520

gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc ttcctgcacg     2580

ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc     2640

tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac ttcaaggtga     2700

tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc cgcagcaacg     2760

ccaccgtgga gcacctgcac cccatgggcg ataacgatct ggatggcagc ttcacccgca     2820

ccttcagcct gcgcgacggc ggctactaca gctccgtggt ggacagccac atgcacttca     2880

agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc ttccgccgcg     2940

tggaggagga tcacagcaac accgagctgg gcatcgtgga gtaccagcac gccttcaaga     3000

ccccggatgc agatgccggt gaagaaagag tttaaacggc cggctgatca taatcagcca     3060

taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct     3120

gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta     3180

caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag     3240

ttgtggtttg tccaaactca tcaatgtatc tta                                  3273


<210>  62
<211>  1859
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  expression cassette for VLP

<400>  62
cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt       60

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      120

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      180

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      240

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      300

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      360

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      420

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      480

acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcca      540

ccatgtactc tttcgtgtct gaggaaaccg gcaccctgat cgtgaacagc gtgctgctgt      600

ttctggcctt cgtggttttc ctgctggtca ccctcgccat cctgaccgcc ctgcggctgt      660

gcgcctactg ctgcaacatc gtgaacgtgt ctctggtcaa acctagcttc tacgtgtata      720

gccgggtgaa gaacctgaat tctagcaggg tgcccgacct gctggtggcc accaacttca      780

gcctgctgaa acaggctggc gatgtggaag agaaccctgg acctgccgat agcaacggca      840

ccattacagt ggaggaactc aaaaagctgc tggaacagtg gaatcttgtg atcggcttcc      900

tgttcctgac ctggatctgc ctgctgcagt tcgcctacgc caaccgcaac agattcctgt      960

acatcatcaa actgatcttc ctgtggctgc tgtggcccgt gaccctggct tgtttcgtgc     1020

tggctgctgt ttatagaatc aactggatca caggcggcat cgcaatcgcc atggcctgtc     1080

tggtgggcct gatgtggctg agctacttca tcgccagctt tagactgttc gctagaacaa     1140

gaagcatgtg gtcctttaac cccgagacaa acatcctcct gaatgtgcca ctgcatggca     1200

ccatcctgac aagacccctg ctggaaagcg agctggtcat cggcgccgtg atcctgcggg     1260

gccacctgag aatcgctggc caccacctgg gcagatgtga catcaaggac ctgcccaagg     1320

aaatcactgt ggccacaagc agaaccctca gctactacaa gctgggagcc tctcagagag     1380

tggccggcga cagcggcttc gccgcctaca gccggtaccg gattggcaat tacaaactga     1440

acaccgacca cagctccagc agcgacaaca tcgctctgct agtgcaggag ggcaggggaa     1500

gtcttctaac atgcggggac gtggaggaaa atcccggccc aagacccaag ctggctagcc     1560

tcgagtctag agggcccgtt taaacccgct gatcagcctc gaggtaccgg atccgcggcc     1620

gcgatatctc tagactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt     1680

gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat     1740

tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag     1800

caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatgg      1859


<210>  63
<211>  6222
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  expression vector with expression cassette for VLP

<400>  63
cccgggaggt accgagctct tacgcgtgct agaattaaag taacccaatc agcacacaat       60

tgccattata cgcgcgtata atggactatt gtgtgctgat aaacctattt cagcatacta      120

cgcgcgtagt atgctgaaat aggtgactag aagttcctat actttctaga gaataggaac      180

ttcataactt cgtataatgt atgctatacg aagttatggg ttactttaat ttggttgctg      240

actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac      300

acctggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg      360

gggactttcc acacccctga ttctgtggat aaccgtatta ccgccatgca ttagttatta      420

atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata      480

acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat      540

aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga      600

gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc      660

ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt      720

atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat      780

gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag      840

tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc      900

aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga      960

ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagcgcc accatgtact     1020

ctttcgtgtc tgaggaaacc ggcaccctga tcgtgaacag cgtgctgctg tttctggcct     1080

tcgtggtttt cctgctggtc accctcgcca tcctgaccgc cctgcggctg tgcgcctact     1140

gctgcaacat cgtgaacgtg tctctggtca aacctagctt ctacgtgtat agccgggtga     1200

agaacctgaa ttctagcagg gtgcccgacc tgctggtggc caccaacttc agcctgctga     1260

aacaggctgg cgatgtggaa gagaaccctg gacctatggc cgatagcaac ggcaccatta     1320

cagtggagga actcaaaaag ctgctggaac agtggaatct tgtgatcggc ttcctgttcc     1380

tgacctggat ctgcctgctg cagttcgcct acgccaaccg caacagattc ctgtacatca     1440

tcaaactgat cttcctgtgg ctgctgtggc ccgtgaccct ggcttgtttc gtgctggctg     1500

ctgtttatag aatcaactgg atcacaggcg gcatcgcaat cgccatggcc tgtctggtgg     1560

gcctgatgtg gctgagctac ttcatcgcca gctttagact gttcgctaga acaagaagca     1620

tgtggtcctt taaccccgag acaaacatcc tcctgaatgt gccactgcat ggcaccatcc     1680

tgacaagacc cctgctggaa agcgagctgg tcatcggcgc cgtgatcctg cggggccacc     1740

tgagaatcgc tggccaccac ctgggcagat gtgacatcaa ggacctgccc aaggaaatca     1800

ctgtggccac aagcagaacc ctcagctact acaagctggg agcctctcag agagtggccg     1860

gcgacagcgg cttcgccgcc tacagccggt accggattgg caattacaaa ctgaacaccg     1920

accacagctc cagcagcgac aacatcgctc tgctagtgca ggccaccaat ttcagcctgc     1980

tgaagcaagc tggagatgtg gaagaaaacc ccggccctcc aaacattacc aacctgtgcc     2040

ccttcggcga ggtgttcaac gccacacggt tcgccagcgt gtacgcctgg aacagaaagc     2100

ggatcagcaa ctgcgtggcc gactacagtg tcctgtataa ctccgccagc ttttctacat     2160

tcaagtgcta cggcgtctcc cctaccaagc tgaacgacct gtgcttcacc aatgtgtacg     2220

ccgattcttt cgtgatcaga ggcgacgagg tgcggcagat cgcccctggc cagaccggaa     2280

agatcgctga ttacaactac aagctgcctg atgacttcac cggctgcgtg atcgcctgga     2340

actccaacaa cctggacagc aaggtggggg gcaactacaa ctacctgtac agactgttca     2400

gaaagagcaa tctgaagcct ttcgagagag atatcagcac agagatctac caggccggca     2460

gcaccccttg taatggcgtt gagggcttca attgctactt tccactgcag agctatggct     2520

ttcagcctac aaacggcgtg ggctaccaac cttacagagt ggtggtgctg tctttcgagc     2580

tgctgcacgc ccctggcgga ggaggaggcg gatctttcat cgaggacctg ctgttcaaca     2640

aggtgaccct ggccgacgcc ggttttggcg gtggcggcgg cggctggcct tggtacatct     2700

ggctgggctt catcgccgga ctgatcgcca tcgtgatggt caccatcatg ctgtgactgt     2760

gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga     2820

aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag     2880

taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga     2940

agacaatagc aggcatgctg gggatgcggt gggctctatg gaagcttacg cgtggccgct     3000

cgagacgcaa ttcggcttgg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc     3060

aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag     3120

gcagaagtat gcaaagcatg catctcaatt agtcagcaac caaattaaag taacccataa     3180

cttcgtatag catacattat acgaagttat gaagttccta ttctctagaa agtataggaa     3240

cttctagtca cctatttcag catactacgc gcgtagtatg ctgaaatagg tttatcagca     3300

cacaatagtc cattatacgc gcgtataatg gcaattgtgt gctgattggg ttactttaat     3360

ttggatccgt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc     3420

gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg     3480

acaggtgccg gcagcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc     3540

ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag     3600

gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa     3660

aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc     3720

gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc     3780

ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg     3840

cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt     3900

cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc     3960

gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc     4020

cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag     4080

agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg     4140

ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa     4200

ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag     4260

gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact     4320

cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa     4380

attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt     4440

accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag     4500

ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca     4560

gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc     4620

agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt     4680

ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg     4740

ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca     4800

gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg     4860

ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca     4920

tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg     4980

tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct     5040

cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca     5100

tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca     5160

gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg     5220

tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac     5280

ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt     5340

attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc     5400

cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg     5460

cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc     5520

ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa     5580

atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac     5640

ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt     5700

tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca     5760

accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt     5820

taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta     5880

caatttgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct     5940

cttcgctatt acgccagccc aagctaccat gataagtaag taatattaag gtacgtggag     6000

gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat     6060

gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc     6120

atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa     6180

ctcatcaatg tatcttatgg tactgtaact gagctaacat aa                        6222


<210>  64
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward Primer

<400>  64
actgctgcaa catcgtgaac                                                   20


<210>  65
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer

<400>  65
tgctagaatt caggttcttc acc                                               23


<210>  66
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer

<400>  66
ttcctgtggc tgctgtgg                                                     18


<210>  67
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer

<400>  67
atgaccagct cgctttccag                                                   20


<210>  68
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward Primer

<400>  68
atcagcacag agatctacca gg                                                22


<210>  69
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer

<400>  69
agcaccacca ctctgtaagg                                                   20


<210>  70
<211>  65
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ACE2 receptor peptide

<400>  70

Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe Asn His 
1               5                   10                  15      


Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp Asn Tyr 
            20                  25                  30          


Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn Ala Gly 
        35                  40                  45              


Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala Gln Met 
    50                  55                  60                  


Tyr 
65  


<210>  71
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BAP tag

<400>  71

Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His Glu 
1               5                   10                  15  


<210>  72
<211>  80
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ACE2 receptor peptide with C-terminal BAP tag

<400>  72

Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe Asn His 
1               5                   10                  15      


Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp Asn Tyr 
            20                  25                  30          


Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn Ala Gly 
        35                  40                  45              


Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala Gln Met 
    50                  55                  60                  


Tyr Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His Glu 
65                  70                  75                  80  


<210>  73
<211>  240
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ACE2 receptor peptide with C-terminal BAP tag

<400>  73
tccactattg aagaacaggc aaagactttc ttggacaaat tcaaccacga ggccgaagac       60

ttgttctatc aaagttccct tgcgagttgg aattacaata cgaatatcac cgaagaaaac      120

gttcagaata tgaacaatgc aggcgacaaa tggtccgcct ttttgaaaga acaaagtacc      180

ctggcccaga tgtacggtct taatgacatc tttgaagcgc aaaagatcga gtggcacgaa      240


<210>  74
<211>  80
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ACE2 receptor peptide with N-terminal BAP tag

<400>  74

Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His Glu Ser 
1               5                   10                  15      


Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe Asn His Glu 
            20                  25                  30          


Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp Asn Tyr Asn 
        35                  40                  45              


Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn Ala Gly Asp 
    50                  55                  60                  


Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala Gln Met Tyr 
65                  70                  75                  80  


<210>  75
<211>  240
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ACE2 receptor peptide with N-terminal BAP tag

<400>  75
ggtcttaatg acatctttga agcgcaaaag atcgagtggc acgaatccac tattgaagaa       60

caggcaaaga ctttcttgga caaattcaac cacgaggccg aagacttgtt ctatcaaagt      120

tcccttgcga gttggaatta caatacgaat atcaccgaag aaaacgttca gaatatgaac      180

aatgcaggcg acaaatggtc cgcctttttg aaagaacaaa gtaccctggc ccagatgtac      240


<210>  76
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ACE2 binding peptide

<400>  76

Gln Ser Tyr Gly Phe Gln Pro Thr Asn 
1               5                   


<210>  77
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ACE2 binding peptide

<400>  77

Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn 
1               5                   10  


<210>  78
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ACE2 binding peptide

<400>  78

Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr 
1               5                   10              


<210>  79
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ACE2 binding peptide

<400>  79

Gln Pro Thr Asn Gly Val Gly Tyr 
1               5               


<210>  80
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ACE2 binding peptide

<400>  80

Phe Gln Pro Thr Asn Gly Val Gly Tyr 
1               5                   


<210>  81
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ACE2 binding peptide

<400>  81

Gln Pro Thr Asn 
1               


<210>  82
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ACE2 binding peptide

<400>  82

Phe Gln Pro Thr Asn 
1               5   


<210>  83
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ACE2 binding peptide

<400>  83

Phe Gln Pro Thr Asn Gly Val 
1               5           


<210>  84
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ACE2 binding peptide

<400>  84

Thr Asn Gly Val Gly Tyr 
1               5       


<210>  85
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ACE2 binding peptide

<400>  85

Phe Asn Cys Tyr Phe Pro Leu Gln 
1               5               


<210>  86
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ACE2 binding peptide

<400>  86

Gly Phe Asn Cys Tyr Phe Pro Leu Gln 
1               5                   


<210>  87
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ACE2 binding peptide

<400>  87

Glu Gly Phe Asn 
1               


<210>  88
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ACE2 binding peptide

<400>  88

Val Glu Gly Phe Asn Cys Tyr 
1               5           


<210>  89
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ACE2 binding peptide

<400>  89

Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln 
1               5                   10  


<210>  90
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ACE2 binding peptide

<400>  90

Tyr Asn Tyr Leu Tyr 
1               5   


<210>  91
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ACE2 binding peptide

<400>  91

Asn Tyr Asn Tyr Leu Tyr Arg 
1               5           


<210>  92
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ACE2 binding peptide

<400>  92

Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala 
1               5                   10                  15      


Gly Phe 
        


<210>  93
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ACE2 binding peptide

<400>  93

Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala 
1               5                   10                  15      


Gly Phe Met Lys Gln Tyr Gly Cys Gly Lys Lys Lys Lys 
            20                  25                  


<210>  94
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ACE2 binding peptide

<400>  94

Ser Phe Ile Glu Asp Leu Leu Phe 
1               5               


<210>  95
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ACE2 binding peptide

<400>  95

Ser Phe Ile Glu Asp Leu Leu Phe Gly Cys Gly Lys Lys Lys Lys 
1               5                   10                  15  


<210>  96
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ACE2 binding peptide

<400>  96

Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala 
1               5                   10                  15      


Gly Phe Met Lys Gln Tyr 
            20          


<210>  97
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ACE2 binding peptide

<400>  97

Ser Phe Ile Glu Asp Ala Ala Ala Gly Cys Gly Lys Lys Lys Lys 
1               5                   10                  15  


<210>  98
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ACE2 binding peptide

<400>  98

Ser Phe Ile Glu Asp Ala Ala Ala 
1               5               


<210>  99
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ACE2 binding peptide

<400>  99

Thr Arg Tyr Tyr Tyr Leu Asn Tyr Asn Tyr Thr Thr Gly Tyr 
1               5                   10                  


<210>  100
<211>  188
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ACE2 binding control peptide

<400>  100

Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn 
1               5                   10                  15      


Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val 
            20                  25                  30          


Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser 
        35                  40                  45              


Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val 
    50                  55                  60                  


Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp 
65                  70                  75                  80  


Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln 
                85                  90                  95      


Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr 
            100                 105                 110         


Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly 
        115                 120                 125             


Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys 
    130                 135                 140                 


Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr 
145                 150                 155                 160 


Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser 
                165                 170                 175     


Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln 
            180                 185             


<210>  101
<211>  576
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  immunogenic sequence

<400>  101
cctaatatta caaacttgtg cccttttggt gaagttttta acgccaccag atttgcatct       60

gtttatgctt ggaacaggaa gagaatcagc aactgtgttg ctgattattc tgtcctatat      120

aattccgcat cattttccac ttttaagtgt tatggagtgt ctcctactaa attaaatgat      180

ctctgcttta ctaatgtcta tgcagattca tttgtaatta gaggtgatga agtcagacaa      240

atcgctccag ggcaaactgg aaagattgct gattataatt ataaattacc agatgatttt      300

acaggctgcg ttatagcttg gaattctaac aatcttgatt ctaaggttgg tggtaattat      360

aattacctgt atagattgtt taggaagtct aatctcaaac cttttgagag agatatttca      420

actgaaatct atcaggccgg tagcacacct tgtaatggtg ttgaaggttt taattgttac      480

tttcctttac aatcatatgg tttccaaccc actaatggtg ttggttacca accatacaga      540

gtagtagtac tttcttttga acttctacat gcacca                                576


<210>  102
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  transmembrane domain

<400>  102

Trp Pro Trp Tyr Ile Trp Leu Gly Phe Ile Ala Gly Leu 
1               5                   10              


<210>  103
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  transmembrane domain

<400>  103
tggccatggt acatttggct aggttttata gctggcttga                             40


<210>  104
<211>  3153
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  bacterial sequence-free vector

<400>  104
cgcgcgtagt atgctgaaat aggtgactag aagttcctat actttctaga gaataggaac       60

ttcataactt cgtataatgt atgctatacg aagttatggg ttactttaat ttggttgctg      120

actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac      180

acctggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg      240

gggactttcc acacccctga ttctgtggat aaccgtatta ccgccatgca ttagttatta      300

atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata      360

acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat      420

aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga      480

gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc      540

ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt      600

atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat      660

gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag      720

tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc      780

aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga      840

ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagcgcc accatgtact      900

ctttcgtgtc tgaggaaacc ggcaccctga tcgtgaacag cgtgctgctg tttctggcct      960

tcgtggtttt cctgctggtc accctcgcca tcctgaccgc cctgcggctg tgcgcctact     1020

gctgcaacat cgtgaacgtg tctctggtca aacctagctt ctacgtgtat agccgggtga     1080

agaacctgaa ttctagcagg gtgcccgacc tgctggtggc caccaacttc agcctgctga     1140

aacaggctgg cgatgtggaa gagaaccctg gacctatggc cgatagcaac ggcaccatta     1200

cagtggagga actcaaaaag ctgctggaac agtggaatct tgtgatcggc ttcctgttcc     1260

tgacctggat ctgcctgctg cagttcgcct acgccaaccg caacagattc ctgtacatca     1320

tcaaactgat cttcctgtgg ctgctgtggc ccgtgaccct ggcttgtttc gtgctggctg     1380

ctgtttatag aatcaactgg atcacaggcg gcatcgcaat cgccatggcc tgtctggtgg     1440

gcctgatgtg gctgagctac ttcatcgcca gctttagact gttcgctaga acaagaagca     1500

tgtggtcctt taaccccgag acaaacatcc tcctgaatgt gccactgcat ggcaccatcc     1560

tgacaagacc cctgctggaa agcgagctgg tcatcggcgc cgtgatcctg cggggccacc     1620

tgagaatcgc tggccaccac ctgggcagat gtgacatcaa ggacctgccc aaggaaatca     1680

ctgtggccac aagcagaacc ctcagctact acaagctggg agcctctcag agagtggccg     1740

gcgacagcgg cttcgccgcc tacagccggt accggattgg caattacaaa ctgaacaccg     1800

accacagctc cagcagcgac aacatcgctc tgctagtgca ggccaccaat ttcagcctgc     1860

tgaagcaagc tggagatgtg gaagaaaacc ccggccctcc aaacattacc aacctgtgcc     1920

ccttcggcga ggtgttcaac gccacacggt tcgccagcgt gtacgcctgg aacagaaagc     1980

ggatcagcaa ctgcgtggcc gactacagtg tcctgtataa ctccgccagc ttttctacat     2040

tcaagtgcta cggcgtctcc cctaccaagc tgaacgacct gtgcttcacc aatgtgtacg     2100

ccgattcttt cgtgatcaga ggcgacgagg tgcggcagat cgcccctggc cagaccggaa     2160

agatcgctga ttacaactac aagctgcctg atgacttcac cggctgcgtg atcgcctgga     2220

actccaacaa cctggacagc aaggtggggg gcaactacaa ctacctgtac agactgttca     2280

gaaagagcaa tctgaagcct ttcgagagag atatcagcac agagatctac caggccggca     2340

gcaccccttg taatggcgtt gagggcttca attgctactt tccactgcag agctatggct     2400

ttcagcctac aaacggcgtg ggctaccaac cttacagagt ggtggtgctg tctttcgagc     2460

tgctgcacgc ccctggcgga ggaggaggcg gatctttcat cgaggacctg ctgttcaaca     2520

aggtgaccct ggccgacgcc ggttttggcg gtggcggcgg cggctggcct tggtacatct     2580

ggctgggctt catcgccgga ctgatcgcca tcgtgatggt caccatcatg ctgtgactgt     2640

gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga     2700

aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag     2760

taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga     2820

agacaatagc aggcatgctg gggatgcggt gggctctatg gaagcttacg cgtggccgct     2880

cgagacgcaa ttcggcttgg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc     2940

aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag     3000

gcagaagtat gcaaagcatg catctcaatt agtcagcaac caaattaaag taacccataa     3060

cttcgtatag catacattat acgaagttat gaagttccta ttctctagaa agtataggaa     3120

cttctagtca cctatttcag catactacgc gcg                                  3153


