               SEQUENCE LISTING

<110> Pirelli Tyre S.p.A.

<120> Manufacture of elastomeric compounds comprising oils with a plasticising action obtained from oleaginous microbial cells

<130> PIR1P82WO

<160> 29

<170> BiSSAP 1.3.6

<210> 1
<211> 1461
<212> DNA
<213> Lipomyces starkeyi


<220> 
<223> Gene encoding for the enzyme delta-9 desaturase

<400> 1
atgactgcca gtgctgagac aacgtccgcg cagcctgtcg tcgagtcggc tcgcgcaagg      60

ccacccagat caagctcaac gtcgccttct cgttcggttg gtagtgctgc gtcgactgcg     120

aagcaagcgt ctcctacatt cgtccacatc tccgagcaac cgttcactct ccagaactgg     180

tacaagcaca tcagctggct caatgtcacg ctgatcatct tcatccctgt cattggctgc     240

actaccgcgg ttttcactcc tctgcaatct aagactgcca tccttgcctt tgtctactac     300

gccctgacgg gcctcggtat cactgcgggt tatcaccgcc tctggtcgca ccgtgcttac     360

agtgcccgtc ttcctctccg tattctactc gctgctttcg gcggcggtgc tgttgagggt     420

tccattcgct ggtggtccgc tggtcaccgt gtccatcaca gattcaccga tactgagaag     480

gacccttact ctgtccgcaa gggtctgctc tattctcaca tgggctggat ggtgtttctc     540

cacaacccca agaagtccgg ccgggtcgat atcaccgact tgaacgctga ccctgtcgtc     600

agatggcagc acaagaacta cattctcgtc cttctcttta tgggtttcat cttccccatg     660

gtagttgccg gcctcggatg gggtgactgg aagggtggtc tcatctgggc tggcattgtc     720

cgtttgacag ttgtccacca tgccactttt tgtgtcaact cgctcgctca ctggctcggt     780

gaccagcctt tcgacgaccg ccgctctccg cgtgaccact tcttgactgc catcgttacg     840

ttcggcgagg gctatcacaa cttccaccat gagttcccct ctgactaccg taacgccata     900

agatggtatc agtatgatcc cactaagtgg ctcatctggt tcctcaagaa gatcggcttt     960

gcttatgacc ttaagacctt ctctcacaat gccatccagc aaggcctcgt ccagcagagg    1020

cagaaaaagc tcgacaagtg gcgcgcacgt cttaactggg gtgttcctct cgagcagctc    1080

ccggtcatgg aatttgaaga gtaccaggag caggccaaga cgcgtgcgct cgtcctcatt    1140

gctggtgttg tccacgatgt caccaacttt attgagcagc atcctggtgg aaaggctctg    1200

atccagtcag gtattggcaa ggatgccacc gctgtcttca atggcggtgt ctacgaccac    1260

tccaatgctg cccacaacct gctcggtacc atgcgtgttg gtgtcattcg cggcggcatg    1320

gaagtcgagg tctggaagat ggctcagcga gagaataagg agtcaacgat caagtccgat    1380

tcgaataatg ccaatatcgt ccgtgcaggt tctcaggcaa cccggataca agctcccatc    1440

cagggcgctg gtgccgctta g                                              1461


<210> 2
<211> 1209
<212> DNA
<213> Lipomyces starkeyi


<220> 
<223> Gene encoding for the enzyme delta-12 desaturase

<400> 2
atgtccacaa taacatacac acagcgcagg ccgtcagtgt cgctgacttc gaagcccgtc      60

tacaaggatg ccttcggcca cgacttcgaa ccgccggagt acacaatcaa agatatcctt     120

gatgccatcc ccaagcactg cttcgaccgc tctcttagcc actctctcgc ctatgtcgcc     180

cgcgacctct tctacgcctc ctgcttgttc ggcctagcga cacagatcca tagcatcccc     240

tatctacctg cccgcgtcgt cgcctgggtt ctctacggct tttgccaagg ccttgtcggc     300

acaggcttgt gggtcctcgc ccacgagtgc ggccatggag ccttctcccc ctacaagctc     360

gccaacgacg tcgtcgggtg gctcctccac tccgccctct ttgtgccgta ccactcatgg     420

cggatcactc actccaagca ccacaaagcc accggccacc tcacccgcga tatggtcttc     480

gtcccgcgcg acgtgacccg ttacaagctc tcccgaaacc tgactgagct caccgaggag     540

gcgccgatcg cgaccctcta tttcctattt atccagcagg tctttggttg gcccgcgtac     600

ctcgcctaca atgtcaccgg ccagaaatac cctggtgtgt ccagcttcag acggtcacat     660

tttgcgccgt ccgcgcccat gttcgatgta aaggacttct gggatattat aatctccgat     720

gtcggtatac ttgtcgccgg cactcttacc tatctcggca tccagaaatg gggttgggct     780

aacttcgctc tctactactt tatcccttat ctctgggtca acaactggtt ggtttgcatc     840

acgtatctac agcacactga cccaactcta ccccattacg acgcgagcga gtggaacttt     900

gcccgcggcg ccgcggcaac agtagatcgc gactttggct tcatcggccg ccacctcttc     960

cacgagatca tcgagacgca tgtcgcgcac cactactcgt cgcgaattcc attctaccac    1020

gccgaggagg ccacgcaagc catccgcaag gtcatgggca agcattaccg tcaggacaag    1080

acaaacctca ttctcgcact ctggaagacc gcgcggacat gccattttgt ggagggcgac    1140

ggcgtcaaga tgtacagaaa tgccaacgga atcggtattc cgccaaagga gggaagaagg    1200

gctcagtaa                                                            1209


<210> 3
<211> 57
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligonucleotide URA3 fw

<400> 3
tcgatccaga attcgtgatt ctagactcga gatatcatga tcactgagcg atagttc        57


<210> 4
<211> 49
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligonucleotide URA3 rev

<400> 4
tcgtcaagag tggtacccat gttgaattta gggatatact gtagaagac                 49


<210> 5
<211> 42
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligonucleotide tGAL1 fw

<400> 5
tgagcatgcc ctgcccctaa agtttagaga tgtacaaggg gt                        42


<210> 6
<211> 39
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligonucleotide tGAL1 rev

<400> 6
gcttgtcgac gaattcagat tgccacgata actttgtgc                            39


<210> 7
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligonucleotide NsrR fw

<400> 7
atgggtacca ctcttgacga c                                              21


<210> 8
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligonucleotide NsrR rev

<400> 8
ttaggggcag ggcatgctca                                                20


<210> 9
<211> 18
<212> DNA
<213> Artificial Sequence


<220> 
<223> Anchored Primer T7

<400> 9
atttaggtga cactatag                                                  18


<210> 10
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Promoter sequencing primer SP6

<400> 10
taatacgact cactataggg                                                20


<210> 11
<211> 42
<212> DNA
<213> Artificial Sequence


<220> 
<223> Promoter TDH3 fw

<400> 11
cgtgattcta gactcgagat ttaatttgct gaagcggttt gc                        42


<210> 12
<211> 49
<212> DNA
<213> Artificial Sequence


<220> 
<223> Promoter TDH3 rev

<400> 12
cgcttagcga tatcactagt agatcttgcg aatgtggatt agagtaaga                 49


<210> 13
<211> 48
<212> DNA
<213> Artificial Sequence


<220> 
<223> Terminator PGK1 fw

<400> 13
actagtgata tcgctaagcg gccggccctc ccgttaatgt tgggattc                  48


<210> 14
<211> 43
<212> DNA
<213> Artificial Sequence


<220> 
<223> Terminator PGK1 rev

<400> 14
tatcgctcag tgatcatgat cctgtcaatt atgctaccac ttg                       43


<210> 15
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligonucleotide LsTDH3 fw

<400> 15
ataataatcc gaactgccgc                                                20


<210> 16
<211> 39
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligonucleotide OLE1 fw

<400> 16
tcagatacta gtatgactgc cagtgctgag acaacgtcc                            39


<210> 17
<211> 32
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligonucleotide OLE1 rev

<400> 17
agatacacta gtctaagcgg caccagcgcc ct                                   32


<210> 18
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligonucleotide HygR fw

<400> 18
atgggtaaaa agcctgaact cacc                                           24


<210> 19
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligonucleotide HygR rev

<400> 19
ttattccttt gccctcggac g                                              21


<210> 20
<211> 35
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligonucleotide FA2 fw

<400> 20
ctagtaaact agtatgtcca caataacata cacac                                35


<210> 21
<211> 30
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligonucleotide FAD2 rev

<400> 21
tattctacta gtttactgag cccttcttcc                                     30


<210> 22
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligonucleotide OLE1 CNT rev

<400> 22
caactaccat ggggaagatg                                                20


<210> 23
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligonucleotide FAD2 CNT rev

<400> 23
caagtatacc gacatcggag atta                                           24


<210> 24
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligonucleotide OLE1 RT fw

<400> 24
ccacttcttg actgccatgc                                                20


<210> 25
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligonucleotide OLE1 RT rev

<400> 25
gaggaaccag atgagccact                                                20


<210> 26
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligonucleotide FAD2 RT fw

<400> 26
gatcgcgact ttggcttcat                                                20


<210> 27
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligonucleotide FAD2 RT rev

<400> 27
gaattcgcga cgagtagtgg                                                20


<210> 28
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligonucleotide ACT fw

<400> 28
cattgccgac agaatgcaga                                                20


<210> 29
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligonucleotide ACT rev

<400> 29
acggagtact tacgctcagg                                                20