                         SEQUENCE LISTING

<110>  Oxford University Innovation Limited
 
<120>  Method

<130>  N418549WO

<150>  GB2004863.3
<151>  2020-04-02

<160>  52    

<170>  PatentIn version 3.5

<210>  1
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signature peptide of TDP-43 (PN)

<400>  1

Ile Arg Val Thr Glu Asp Glu Asn Asp Glu Pro Ile Glu Ile Pro Ser 
1               5                   10                  15      


Glu Asp Asp Gly Thr Val Leu 
            20              


<210>  2
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signature peptide of TDP-43 (PC)

<400>  2

Ser Gly Ser Asn Ser Gly Ala Ala Ile Gly Trp 
1               5                   10      


<210>  3
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signature peptide of TDP-43 (PT2)

<400>  3

Ser Asn Arg Gln Leu Glu Arg Ser Gly Arg Phe 
1               5                   10      


<210>  4
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  isotope labelled amino acid of signature peptide of TDP-43 (PN)


<220>
<221>  SITE
<222>  (2)..(2)
<223>  isotope labelled amino acid

<400>  4

Ile Arg Val Thr Glu Asp Glu Asn Asp Glu Pro Ile Glu Ile Pro Ser 
1               5                   10                  15      


Glu Asp Asp Gly Thr Val Leu 
            20              


<210>  5
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  isotope labelled amino acid of signature peptide of TDP-43 (PC)


<220>
<221>  SITE
<222>  (6)..(6)
<223>  isotope labelled amino acid

<400>  5

Ser Gly Ser Asn Ser Gly Ala Ala Ile Gly Trp 
1               5                   10      


<210>  6
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  isotope labelled amino acid of signature peptide of TDP-43 (PT2)


<220>
<221>  SITE
<222>  (10)..(10)
<223>  isotope labelled amino acid

<400>  6

Ser Asn Arg Gln Leu Glu Arg Ser Gly Arg Phe 
1               5                   10      


<210>  7
<211>  149
<212>  PRT
<213>  Homo sapiens

<400>  7

Ser Asn Arg Gln Leu Glu Arg Ser Gly Arg Phe Gly Gly Asn Pro Gly 
1               5                   10                  15      


Gly Phe Gly Asn Gln Gly Gly Phe Gly Asn Ser Arg Gly Gly Gly Ala 
            20                  25                  30          


Gly Leu Gly Asn Asn Gln Gly Ser Asn Met Gly Gly Gly Met Asn Phe 
        35                  40                  45              


Gly Ala Phe Ser Ile Asn Pro Ala Met Met Ala Ala Ala Gln Ala Ala 
    50                  55                  60                  


Leu Gln Ser Ser Trp Gly Met Met Gly Met Leu Ala Ser Gln Gln Asn 
65                  70                  75                  80  


Gln Ser Gly Pro Ser Gly Asn Asn Gln Asn Gln Gly Asn Met Gln Arg 
                85                  90                  95      


Glu Pro Asn Gln Ala Phe Gly Ser Gly Asn Asn Ser Tyr Ser Gly Ser 
            100                 105                 110         


Asn Ser Gly Ala Ala Ile Gly Trp Gly Ser Ala Ser Asn Ala Gly Ser 
        115                 120                 125             


Gly Ser Gly Phe Asn Gly Gly Phe Gly Ser Ser Met Asp Ser Lys Ser 
    130                 135                 140                 


Ser Gly Trp Gly Met 
145                 


<210>  8
<211>  414
<212>  PRT
<213>  Homo sapiens

<400>  8

Met Ser Glu Tyr Ile Arg Val Thr Glu Asp Glu Asn Asp Glu Pro Ile 
1               5                   10                  15      


Glu Ile Pro Ser Glu Asp Asp Gly Thr Val Leu Leu Ser Thr Val Thr 
            20                  25                  30          


Ala Gln Phe Pro Gly Ala Cys Gly Leu Arg Tyr Arg Asn Pro Val Ser 
        35                  40                  45              


Gln Cys Met Arg Gly Val Arg Leu Val Glu Gly Ile Leu His Ala Pro 
    50                  55                  60                  


Asp Ala Gly Trp Gly Asn Leu Val Tyr Val Val Asn Tyr Pro Lys Asp 
65                  70                  75                  80  


Asn Lys Arg Lys Met Asp Glu Thr Asp Ala Ser Ser Ala Val Lys Val 
                85                  90                  95      


Lys Arg Ala Val Gln Lys Thr Ser Asp Leu Ile Val Leu Gly Leu Pro 
            100                 105                 110         


Trp Lys Thr Thr Glu Gln Asp Leu Lys Glu Tyr Phe Ser Thr Phe Gly 
        115                 120                 125             


Glu Val Leu Met Val Gln Val Lys Lys Asp Leu Lys Thr Gly His Ser 
    130                 135                 140                 


Lys Gly Phe Gly Phe Val Arg Phe Thr Glu Tyr Glu Thr Gln Val Lys 
145                 150                 155                 160 


Val Met Ser Gln Arg His Met Ile Asp Gly Arg Trp Cys Asp Cys Lys 
                165                 170                 175     


Leu Pro Asn Ser Lys Gln Ser Gln Asp Glu Pro Leu Arg Ser Arg Lys 
            180                 185                 190         


Val Phe Val Gly Arg Cys Thr Glu Asp Met Thr Glu Asp Glu Leu Arg 
        195                 200                 205             


Glu Phe Phe Ser Gln Tyr Gly Asp Val Met Asp Val Phe Ile Pro Lys 
    210                 215                 220                 


Pro Phe Arg Ala Phe Ala Phe Val Thr Phe Ala Asp Asp Gln Ile Ala 
225                 230                 235                 240 


Gln Ser Leu Cys Gly Glu Asp Leu Ile Ile Lys Gly Ile Ser Val His 
                245                 250                 255     


Ile Ser Asn Ala Glu Pro Lys His Asn Ser Asn Arg Gln Leu Glu Arg 
            260                 265                 270         


Ser Gly Arg Phe Gly Gly Asn Pro Gly Gly Phe Gly Asn Gln Gly Gly 
        275                 280                 285             


Phe Gly Asn Ser Arg Gly Gly Gly Ala Gly Leu Gly Asn Asn Gln Gly 
    290                 295                 300                 


Ser Asn Met Gly Gly Gly Met Asn Phe Gly Ala Phe Ser Ile Asn Pro 
305                 310                 315                 320 


Ala Met Met Ala Ala Ala Gln Ala Ala Leu Gln Ser Ser Trp Gly Met 
                325                 330                 335     


Met Gly Met Leu Ala Ser Gln Gln Asn Gln Ser Gly Pro Ser Gly Asn 
            340                 345                 350         


Asn Gln Asn Gln Gly Asn Met Gln Arg Glu Pro Asn Gln Ala Phe Gly 
        355                 360                 365             


Ser Gly Asn Asn Ser Tyr Ser Gly Ser Asn Ser Gly Ala Ala Ile Gly 
    370                 375                 380                 


Trp Gly Ser Ala Ser Asn Ala Gly Ser Gly Ser Gly Phe Asn Gly Gly 
385                 390                 395                 400 


Phe Gly Ser Ser Met Asp Ser Lys Ser Ser Gly Trp Gly Met 
                405                 410                 


<210>  9
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signature peptide of TDP-43 (PT1)

<400>  9

Lys Leu Pro Asn Ser Lys Gln Ser Gln Asp Glu Pro Leu 
1               5                   10              


<210>  10
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  isotope labelled amino acid of signature peptide of TDP-43 (PT1)


<220>
<221>  SITE
<222>  (1)..(1)
<223>  isotope labelled amino acid

<400>  10

Lys Leu Pro Asn Ser Lys Gln Ser Gln Asp Glu Pro Leu 
1               5                   10              


<210>  11
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signature peptide of TDP-43

<400>  11

Thr Ser Asp Leu Ile Val Leu Gly Leu Pro Trp Lys 
1               5                   10          


<210>  12
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  isotope labelled amino acid of signature peptide of TDP-43


<220>
<221>  SITE
<222>  (12)..(12)
<223>  isotope labelled amino acid

<400>  12

Thr Ser Asp Leu Ile Val Leu Gly Leu Pro Trp Lys 
1               5                   10          


<210>  13
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signature peptide of TDP-43

<400>  13

Phe Gly Gly Asn Pro Gly Gly Phe Gly Asn Gln Gly Gly Phe Gly Asn 
1               5                   10                  15      


Ser Arg 
        


<210>  14
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  isotope labelled amino acid of signature peptide of TDP-43


<220>
<221>  SITE
<222>  (18)..(18)
<223>  isotope labelled amino acid

<400>  14

Phe Gly Gly Asn Pro Gly Gly Phe Gly Asn Gln Gly Gly Phe Gly Asn 
1               5                   10                  15      


Ser Arg 
        


<210>  15
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signature peptide of TDP-43

<400>  15

Phe Gly Gly Asn Pro Gly Gly Phe Gly Asn Gln Gly Gly Phe Gly Asn 
1               5                   10                  15      


<210>  16
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  isotope labelled amino acid of signature peptide of TDP-43


<220>
<221>  SITE
<222>  (6)..(6)
<223>  isotope labelled amino acid

<400>  16

Phe Gly Gly Asn Pro Gly Gly Phe Gly Asn Gln Gly Gly Phe Gly Asn 
1               5                   10                  15      


<210>  17
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signature peptide of TDP-43

<400>  17

Pro Gly Gly Phe Gly Asn Gln Gly Gly Phe Gly Asn Ser Arg 
1               5                   10                  


<210>  18
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  isotope labelled amino acid of signature peptide of TDP-43


<220>
<221>  SITE
<222>  (14)..(14)
<223>  isotope labelled amino acid

<400>  18

Pro Gly Gly Phe Gly Asn Gln Gly Gly Phe Gly Asn Ser Arg 
1               5                   10                  


<210>  19
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signature peptide of TDP-43

<400>  19

Ser Ser Gly Trp Gly Met 
1               5       


<210>  20
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  isotope labelled amino acid of signature peptide of TDP-43


<220>
<221>  SITE
<222>  (3)..(3)
<223>  isotope labelled amino acid

<400>  20

Ser Ser Gly Trp Gly Met 
1               5       


<210>  21
<211>  317
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TDP-43 QconCat Construct

<400>  21

His His His His His His Leu Lys Ser Trp Pro Glu Ser Asp Ala Thr 
1               5                   10                  15      


Val Asp Ala Phe Lys Ala Asp Gly Thr Val Pro Glu Ser Ser Gly Ala 
            20                  25                  30          


Gly Leu Arg Val Ala Tyr Pro Ser Ala Ala Asp Ile Gly Asp Tyr Arg 
        35                  40                  45              


Val Ile Ala Ala Pro Asp Ile Ile Thr Gly Phe Arg Thr Val Gln Glu 
    50                  55                  60                  


Asn Gly Gly Leu Arg Ile Val Gly Gly Pro Asp Ile Ile Thr Gly Phe 
65                  70                  75                  80  


Arg Tyr Pro Glu Gly Gly Ser Ile Val Thr Pro Tyr Arg Tyr Pro Glu 
                85                  90                  95      


Ala Gly Thr Ile Val Pro Thr Tyr Lys Ala Asn Ser Glu Asp Val Gly 
            100                 105                 110         


Thr Glu Ala Val Phe Lys Asn Ser Glu Val Gly Ala Ile Ile Tyr Ala 
        115                 120                 125             


Ala Glu Asp Ala Thr Asn Glu Lys Gln Ala Leu Ser Arg Glu Asp Ala 
    130                 135                 140                 


Glu Gly Arg Leu Thr Ser Val Gly Ser Ile Thr Ser Gly Tyr Glu Tyr 
145                 150                 155                 160 


Pro Gly Ser Gln Gly Ser Pro Ala Val Asp Lys Glu Arg Phe Glu Val 
                165                 170                 175     


Asp Lys Ile Ala Gln Asn Leu Ala Ser Ser Ser Asp Thr Arg Val Gly 
            180                 185                 190         


Ala Pro Ala Thr Gly Ser Gly Thr Pro Gly Pro Phe Thr Arg Thr Ser 
        195                 200                 205             


Val Ser Ser Val Ser Ala Ser Pro Ser Arg Phe Arg Gly Ala Gly Ala 
    210                 215                 220                 


Ala Ser Ser Thr Asp Ser Leu Ala Gln Glu Ala Glu Ala Ala Arg Val 
225                 230                 235                 240 


Asp Leu Ile Arg Val Thr Glu Asp Glu Asn Asp Glu Pro Ile Glu Ile 
                245                 250                 255     


Pro Ser Glu Asp Asp Gly Thr Val Leu Ser Gly Ser Asn Ser Gly Ala 
            260                 265                 270         


Ala Ile Gly Trp Lys Leu Pro Asn Ser Lys Gln Ser Gln Asp Glu Pro 
        275                 280                 285             


Leu Ser Asn Arg Gln Leu Glu Arg Ser Gly Arg Phe Ile Ile Lys Gly 
    290                 295                 300                 


Ile Ser Val His Ile Ser Asn Ala Glu Pro Lys His Asn 
305                 310                 315         


<210>  22
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signature peptide of human NFL

<400>  22

Ala Ala Glu Asp Ala Thr Asn Glu Lys Gln Ala Leu 
1               5                   10          


<210>  23
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signature peptide of human NFL

<400>  23

Ser Arg Glu Asp Ala Glu Gly Arg Leu 
1               5                   


<210>  24
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signature peptide of human NFL

<400>  24

Thr Ser Val Gly Ser Ile Thr Ser Gly Tyr 
1               5                   10  


<210>  25
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signature peptide of human NFL

<400>  25

Ser Ala Pro Val Ser Ser Ser Leu 
1               5               


<210>  26
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signature peptide of human NFL

<400>  26

Ser Gln Val Ala Ala Ile Ser Asn Asp Leu 
1               5                   10  


<210>  27
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signature peptide of human NFL

<400>  27

Lys Ser Ile Arg Thr Gln Glu Lys Ala Gln Leu 
1               5                   10      


<210>  28
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signature peptide of human NFL

<400>  28

Arg Gln Lys His Ser Glu Pro Ser Arg Phe 
1               5                   10  


<210>  29
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signature peptide of human CHIT1

<400>  29

Glu Tyr Pro Gly Ser Gln Gly Ser Pro Ala Val Asp Lys Glu Arg Phe 
1               5                   10                  15      


<210>  30
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signature peptide of human CHIT1

<400>  30

Glu Val Asp Lys Ile Ala Gln Asn Leu 
1               5                   


<210>  31
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signature peptide of human CHIT1

<400>  31

Ala Ser Ser Ser Asp Thr Arg Val Gly Ala Pro Ala Thr Gly Ser Gly 
1               5                   10                  15      


Thr Pro Gly Pro Phe 
            20      


<210>  32
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signature peptide of human CHIT1

<400>  32

Lys Gly Ala Thr Lys Gln Arg Ile Gln Asp Gln Lys Val Pro Tyr 
1               5                   10                  15  


<210>  33
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signature peptide of human CHIT1

<400>  33

Leu Pro Ser Gly Thr Pro Glu Leu 
1               5               


<210>  34
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signature peptide of human CHIT1

<400>  34

Glu Val Pro Lys Pro Gly Gln Pro Ser Glu Pro Glu His Gly Pro Ser 
1               5                   10                  15      


Pro Gly Gln Asp Thr Phe 
            20          


<210>  35
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signature peptide of human NFH

<400>  35

Thr Arg Thr Ser Val Ser Ser Val Ser Ala Ser Pro Ser Arg Phe 
1               5                   10                  15  


<210>  36
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signature peptide of human NFH

<400>  36

Arg Gly Ala Gly Ala Ala Ser Ser Thr Asp Ser Leu 
1               5                   10          


<210>  37
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signature peptide of human NFH

<400>  37

Ala Gln Glu Ala Glu Ala Ala Arg Val Asp Leu 
1               5                   10      


<210>  38
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signature peptide of human NFH

<400>  38

Ala Arg Lys Gly Gly Ala Gly Gly Thr Arg Ser Ala Ala Gly Ser Ser 
1               5                   10                  15      


Ser Gly Phe 
            


<210>  39
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signature peptide of human NFH

<400>  39

Asp Asp Glu Ala Arg Gln Arg Glu Glu Ala Glu Ala Ala Ala Arg Ala 
1               5                   10                  15      


Leu 
    


<210>  40
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signature peptide of human NFH

<400>  40

Glu Gly His Ala Val Gln Ser Thr Leu 
1               5                   


<210>  41
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signature peptide of human NFH

<400>  41

Glu Asp Arg His Gln Ala Asp Ile Ala Ser Tyr 
1               5                   10      


<210>  42
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  a retention time standard for mass spectrometry

<400>  42

Lys Ser Trp Pro Glu Ser Asp Ala Thr Val Asp Ala Phe 
1               5                   10              


<210>  43
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  a retention time standard for mass spectrometry

<400>  43

Lys Ala Asp Gly Thr Val Pro Glu Ser Ser Gly Ala Gly Leu 
1               5                   10                  


<210>  44
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  a retention time standard for mass spectrometry

<400>  44

Arg Val Ala Tyr Pro Ser Ala Ala Asp Ile Gly Asp Tyr 
1               5                   10              


<210>  45
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  a retention time standard for mass spectrometry

<400>  45

Arg Val Ile Ala Ala Pro Asp Ile Ile Thr Gly Phe 
1               5                   10          


<210>  46
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  a retention time standard for mass spectrometry

<400>  46

Arg Thr Val Gln Glu Asn Gly Gly Leu 
1               5                   


<210>  47
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  a retention time standard for mass spectrometry

<400>  47

Arg Ile Val Gly Gly Pro Asp Ile Ile Thr Gly Phe 
1               5                   10          


<210>  48
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  a retention time standard for mass spectrometry

<400>  48

Arg Tyr Pro Glu Gly Gly Ser Ile Val Thr Pro Tyr 
1               5                   10          


<210>  49
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  a retention time standard for mass spectrometry

<400>  49

Arg Tyr Pro Glu Ala Gly Thr Ile Val Pro Thr Tyr 
1               5                   10          


<210>  50
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  a retention time standard for mass spectrometry

<400>  50

Lys Ala Asn Ser Glu Asp Val Gly Thr Glu Ala Val 
1               5                   10          


<210>  51
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  a retention time standard for mass spectrometry

<400>  51

Phe Lys Asn Ser Glu Val Gly Ala Ile Ile Tyr 
1               5                   10      


<210>  52
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signature peptide of TDP-43 (PT3)

<400>  52

Ile Ile Lys Gly Ile Ser Val His Ile Ser Asn Ala Glu Pro Lys His 
1               5                   10                  15      


Asn 
    


