                         SEQUENCE LISTING

<110>  Medigene Immunotherapies GmbH
 
<120>  CD3-fusion protein and uses thereof

<130>  M11528WO / DB

<150>  EP20167540
<151>  2020-04-01

<160>  25    

<170>  PatentIn version 3.5

<210>  1
<211>  21
<212>  PRT
<213>  homo sapiens


<220>
<221>  misc_feature
<223>  CD8 signal peptide domain aa

<400>  1

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro 
            20      


<210>  2
<211>  71
<212>  PRT
<213>  homo sapiens


<220>
<221>  misc_feature
<223>  CD3 delta aa

<400>  2

Phe Lys Ile Pro Ile Glu Glu Leu Glu Asp Arg Val Phe Val Asn Cys 
1               5                   10                  15      


Asn Thr Ser Ile Thr Trp Val Glu Gly Thr Val Gly Thr Leu Leu Ser 
            20                  25                  30          


Asp Ile Thr Arg Leu Asp Leu Gly Lys Arg Ile Leu Asp Pro Arg Gly 
        35                  40                  45              


Ile Tyr Arg Cys Asn Gly Thr Asp Ile Tyr Lys Asp Lys Glu Ser Thr 
    50                  55                  60                  


Val Gln Val His Tyr Arg Met 
65                  70      


<210>  3
<211>  81
<212>  PRT
<213>  homo sapiens


<220>
<221>  misc_feature
<223>  CD3 gamma aa

<400>  3

Gln Ser Ile Lys Gly Asn His Leu Val Lys Val Tyr Asp Tyr Gln Glu 
1               5                   10                  15      


Asp Gly Ser Val Leu Leu Thr Cys Asp Ala Glu Ala Lys Asn Ile Thr 
            20                  25                  30          


Trp Phe Lys Asp Gly Lys Met Ile Gly Phe Leu Thr Glu Asp Lys Lys 
        35                  40                  45              


Lys Trp Asn Leu Gly Ser Asn Ala Lys Asp Pro Arg Gly Met Tyr Gln 
    50                  55                  60                  


Cys Lys Gly Ser Gln Asn Lys Ser Lys Pro Leu Gln Val Tyr Tyr Arg 
65                  70                  75                  80  


Met 
    


<210>  4
<211>  96
<212>  PRT
<213>  homo sapiens


<220>
<221>  misc_feature
<223>  CD3 epsilon aa

<400>  4

Asp Gly Asn Glu Glu Met Gly Gly Ile Thr Gln Thr Pro Tyr Lys Val 
1               5                   10                  15      


Ser Ile Ser Gly Thr Thr Val Ile Leu Thr Cys Pro Gln Tyr Pro Gly 
            20                  25                  30          


Ser Glu Ile Leu Trp Gln His Asn Asp Lys Asn Ile Gly Gly Asp Glu 
        35                  40                  45              


Asp Asp Lys Asn Ile Gly Ser Asp Glu Asp His Leu Ser Leu Lys Glu 
    50                  55                  60                  


Phe Ser Glu Leu Glu Gln Ser Gly Tyr Tyr Val Cys Tyr Pro Arg Gly 
65                  70                  75                  80  


Ser Lys Pro Glu Asp Ala Asn Phe Tyr Leu Tyr Leu Arg Ala Arg Val 
                85                  90                  95      


<210>  5
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (G4S) 3 linker aa

<400>  5

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  6
<211>  45
<212>  PRT
<213>  homo sapiens


<220>
<221>  misc_feature
<223>  CD8 hinge aa

<400>  6

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
        35                  40                  45  


<210>  7
<211>  30
<212>  PRT
<213>  homo sapiens


<220>
<221>  misc_feature
<223>  CD28 transmembrane aa

<400>  7

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys 
            20                  25                  30  


<210>  8
<211>  113
<212>  PRT
<213>  homo sapiens


<220>
<221>  misc_feature
<223>  CD 3 zeta aa

<400>  8

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
    50                  55                  60                  


Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu 
65                  70                  75                  80  


Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
                85                  90                  95      


Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro 
            100                 105                 110         


Arg 
    


<210>  9
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Spacer P2A

<400>  9

Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
1               5                   10                  15      


Glu Glu Asn Pro Gly Pro 
            20          


<210>  10
<211>  239
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  eGFP

<400>  10

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235                 


<210>  11
<211>  652
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD3-Chimera complete sequence aa

<400>  11

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Phe Lys Ile Pro Ile Glu Glu Leu Glu Asp Arg 
            20                  25                  30          


Val Phe Val Asn Cys Asn Thr Ser Ile Thr Trp Val Glu Gly Thr Val 
        35                  40                  45              


Gly Thr Leu Leu Ser Asp Ile Thr Arg Leu Asp Leu Gly Lys Arg Ile 
    50                  55                  60                  


Leu Asp Pro Arg Gly Ile Tyr Arg Cys Asn Gly Thr Asp Ile Tyr Lys 
65                  70                  75                  80  


Asp Lys Glu Ser Thr Val Gln Val His Tyr Arg Met Gly Gly Gly Gly 
                85                  90                  95      


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Gly Asn Glu Glu 
            100                 105                 110         


Met Gly Gly Ile Thr Gln Thr Pro Tyr Lys Val Ser Ile Ser Gly Thr 
        115                 120                 125             


Thr Val Ile Leu Thr Cys Pro Gln Tyr Pro Gly Ser Glu Ile Leu Trp 
    130                 135                 140                 


Gln His Asn Asp Lys Asn Ile Gly Gly Asp Glu Asp Asp Lys Asn Ile 
145                 150                 155                 160 


Gly Ser Asp Glu Asp His Leu Ser Leu Lys Glu Phe Ser Glu Leu Glu 
                165                 170                 175     


Gln Ser Gly Tyr Tyr Val Cys Tyr Pro Arg Gly Ser Lys Pro Glu Asp 
            180                 185                 190         


Ala Asn Phe Tyr Leu Tyr Leu Arg Ala Arg Val Thr Thr Thr Pro Ala 
        195                 200                 205             


Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser 
    210                 215                 220                 


Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr 
225                 230                 235                 240 


Arg Gly Leu Asp Phe Ala Cys Asp Phe Trp Val Leu Val Val Val Gly 
                245                 250                 255     


Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile 
            260                 265                 270         


Phe Trp Val Arg Ser Lys Arg Val Lys Phe Ser Arg Ser Ala Asp Ala 
        275                 280                 285             


Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu 
    290                 295                 300                 


Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp 
305                 310                 315                 320 


Pro Glu Met Gly Gly Lys Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly 
                325                 330                 335     


Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu 
            340                 345                 350         


Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu 
        355                 360                 365             


Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His 
    370                 375                 380                 


Met Gln Ala Leu Pro Pro Arg Gly Ser Gly Ala Thr Asn Phe Ser Leu 
385                 390                 395                 400 


Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro Met Val Ser 
                405                 410                 415     


Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu 
            420                 425                 430         


Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu 
        435                 440                 445             


Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr 
    450                 455                 460                 


Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr 
465                 470                 475                 480 


Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln His Asp 
                485                 490                 495     


Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile 
            500                 505                 510         


Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe 
        515                 520                 525             


Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe 
    530                 535                 540                 


Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn 
545                 550                 555                 560 


Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys 
                565                 570                 575     


Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu 
            580                 585                 590         


Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu 
        595                 600                 605             


Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp 
    610                 615                 620                 


Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala 
625                 630                 635                 640 


Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
                645                 650         


<210>  12
<211>  63
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8 signal peptide domain nt (codon optimized)

<400>  12

Ala Thr Gly Gly Cys Cys Cys Thr Gly Cys Cys Thr Gly Thr Gly Ala 
1               5                   10                  15      


Cys Ala Gly Cys Thr Cys Thr Gly Cys Thr Gly Cys Thr Gly Cys Cys 
            20                  25                  30          


Thr Cys Thr Gly Gly Cys Cys Cys Thr Gly Cys Thr Gly Cys Thr Gly 
        35                  40                  45              


Cys Ala Thr Gly Cys Cys Gly Cys Cys Ala Gly Ala Cys Cys Cys 
    50                  55                  60              


<210>  13
<211>  213
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD3 delta nt (codon optimized)

<400>  13
ttcaagatcc ccatcgagga actggaagat cgggtgttcg tgaactgcaa caccagcatc       60

acctgggtgg aaggcaccgt gggcacactg ctgagcgaca tcaccagact ggacctgggc      120

aagagaatcc tggaccccag aggcatctac agatgcaacg gcaccgacat ctacaaggac      180

aaagaaagca ccgtgcaggt gcactaccgg atg                                   213


<210>  14
<211>  243
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD3 gamma nt (codon optimized)

<400>  14
cagtcaatca aaggaaacca cttggttaag gtgtatgact atcaagaaga tggttcggta       60

cttctgactt gtgatgcaga agccaaaaat atcacatggt ttaaagatgg gaagatgatc      120

ggcttcctaa ctgaagataa aaaaaaatgg aatctgggaa gtaatgccaa ggaccctcga      180

gggatgtatc agtgtaaagg atcacagaac aagtcaaaac cactccaagt gtattacaga      240

atg                                                                    243


<210>  15
<211>  288
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD3 epsilon nt (codon optimized)

<400>  15
gatggcaacg aggaaatggg cggcatcacc cagaccccct acaaggtgtc gatatctggc       60

accaccgtga tcctgacctg cccccagtat cccggcagcg agattctgtg gcagcacaac      120

gacaagaaca tcggcggcga cgaggacgat aagaatatcg gctccgatga ggaccacctg      180

agcctgaaag agttcagcga gctggaacag agcggctact acgtgtgcta ccccagggga      240

tccaagcccg aggacgccaa cttctacctg tacctgcggg ccagagtg                   288


<210>  16
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (G4S) 3 linker nt (codon optimized)

<400>  16
ggcggaggcg gatctggcgg cggaggatca gggggagggg gctct                       45


<210>  17
<211>  135
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD8 hinge nt (codon optimized)

<400>  17
accaccaccc ctgctcctag accacctacc ccagccccta caatcgccag ccagcctctg       60

tctctgaggc ccgaggcttg tagaccagct gctggcggag ccgtgcacac cagaggactg      120

gatttcgcct gcgac                                                       135


<210>  18
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD28 transmembrane nt (codon optimized)

<400>  18
ttctgggtgc tggtggtcgt gggcggcgtg ctagcttgtt acagcctgct cgtgaccgtg       60

gccttcatca tcttttgggt gcgcagcaag                                        90


<210>  19
<211>  339
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD 3 zeta nt (codon optimized)

<400>  19
cgcgtgaagt tcagcagaag cgccgacgcc cctgcctacc agcagggaca gaaccagctg       60

tacaacgagc tgaacctggg tcgacgagag gaatacgacg tgctggacaa gcggagaggc      120

cgggaccctg agatgggagg aaagccccag cggagaaaga acccccagga aggcctgtat      180

aacgaactgc agaaagacaa gatggccgag gcctacagcg agatcggaat gaagggcgag      240

cgcagaagag gcaagggaca cgatggactg taccagggac tgagcaccgc caccaaggat      300

acctatgacg ccctgcacat gcaggccctt ccacctaga                             339


<210>  20
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Spacer P2A nt (codon optimized)

<400>  20
ggttccggag ccaccaactt tagcctgctg aaacaggccg gcgacgtgga agagaaccct       60

ggacct                                                                  66


<210>  21
<211>  720
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  eGFP nt (codon optimized)

<400>  21
atggtgtcca aaggcgagga actgttcacc ggcgtggtgc ccattctggt ggaactggac       60

ggggatgtga acggccacaa gtttagcgtt agcggcgaag gcgaagggga tgccacatac      120

ggaaagctga ccctgaagtt catctgcacc accggcaagc tgcctgtgcc ttggcctaca      180

ctggtcacca cactgacata cggcgtgcag tgctttagca gataccccga ccatatgaag      240

cagcacgact tcttcaagag cgccatgcct gagggctacg tgcaagagcg gaccatcttc      300

tttaaggacg acggcaacta caagaccaga gccgaagtga agtttgaggg cgacaccctg      360

gtcaaccgga tcgagctgaa gggcatcgac ttcaaagagg acggcaacat cctgggccac      420

aagctcgagt acaactacaa ctcccacaac gtgtacatca tggccgacaa gcagaaaaac      480

ggcatcaaag tgaacttcaa gatccggcac aacatcgagg acggctctgt gcagctggcc      540

gatcactacc agcagaacac acccatcgga gatggccctg tgctgctgcc cgataaccac      600

tacctgagca cacagagcgc cctgtctaag gaccccaacg agaagaggga ccacatggtg      660

ctgctcgagt ttgtgacagc cgccggaatc accctcggca tggacgagct gtataagtga      720


<210>  22
<211>  1959
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD3-Chimera complete sequence nt delta/epsilon (codon optimized)

<400>  22
atggccctgc ctgtgacagc tctgctgctg cctctggccc tgctgctgca tgccgccaga       60

cccttcaaga tccccatcga ggaactggaa gatcgggtgt tcgtgaactg caacaccagc      120

atcacctggg tggaaggcac cgtgggcaca ctgctgagcg acatcaccag actggacctg      180

ggcaagagaa tcctggaccc cagaggcatc tacagatgca acggcaccga catctacaag      240

gacaaagaaa gcaccgtgca ggtgcactac cggatgggcg gaggcggatc tggcggcgga      300

ggatcagggg gagggggctc tgatggcaac gaggaaatgg gcggcatcac ccagaccccc      360

tacaaggtgt cgatatctgg caccaccgtg atcctgacct gcccccagta tcccggcagc      420

gagattctgt ggcagcacaa cgacaagaac atcggcggcg acgaggacga taagaatatc      480

ggctccgatg aggaccacct gagcctgaaa gagttcagcg agctggaaca gagcggctac      540

tacgtgtgct accccagggg atccaagccc gaggacgcca acttctacct gtacctgcgg      600

gccagagtga ccaccacccc tgctcctaga ccacctaccc cagcccctac aatcgccagc      660

cagcctctgt ctctgaggcc cgaggcttgt agaccagctg ctggcggagc cgtgcacacc      720

agaggactgg atttcgcctg cgacttctgg gtgctggtgg tcgtgggcgg cgtgctagct      780

tgttacagcc tgctcgtgac cgtggccttc atcatctttt gggtgcgcag caagcgcgtg      840

aagttcagca gaagcgccga cgcccctgcc taccagcagg gacagaacca gctgtacaac      900

gagctgaacc tgggtcgacg agaggaatac gacgtgctgg acaagcggag aggccgggac      960

cctgagatgg gaggaaagcc ccagcggaga aagaaccccc aggaaggcct gtataacgaa     1020

ctgcagaaag acaagatggc cgaggcctac agcgagatcg gaatgaaggg cgagcgcaga     1080

agaggcaagg gacacgatgg actgtaccag ggactgagca ccgccaccaa ggatacctat     1140

gacgccctgc acatgcaggc ccttccacct agaggttccg gagccaccaa ctttagcctg     1200

ctgaaacagg ccggcgacgt ggaagagaac cctggaccta tggtgtccaa aggcgaggaa     1260

ctgttcaccg gcgtggtgcc cattctggtg gaactggacg gggatgtgaa cggccacaag     1320

tttagcgtta gcggcgaagg cgaaggggat gccacatacg gaaagctgac cctgaagttc     1380

atctgcacca ccggcaagct gcctgtgcct tggcctacac tggtcaccac actgacatac     1440

ggcgtgcagt gctttagcag ataccccgac catatgaagc agcacgactt cttcaagagc     1500

gccatgcctg agggctacgt gcaagagcgg accatcttct ttaaggacga cggcaactac     1560

aagaccagag ccgaagtgaa gtttgagggc gacaccctgg tcaaccggat cgagctgaag     1620

ggcatcgact tcaaagagga cggcaacatc ctgggccaca agctcgagta caactacaac     1680

tcccacaacg tgtacatcat ggccgacaag cagaaaaacg gcatcaaagt gaacttcaag     1740

atccggcaca acatcgagga cggctctgtg cagctggccg atcactacca gcagaacaca     1800

cccatcggag atggccctgt gctgctgccc gataaccact acctgagcac acagagcgcc     1860

ctgtctaagg accccaacga gaagagggac cacatggtgc tgctcgagtt tgtgacagcc     1920

gccggaatca ccctcggcat ggacgagctg tataagtga                            1959


<210>  23
<211>  662
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD3-Chimera complete sequence aa gamma/epsilon (codon optimized)

<400>  23

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Gln Ser Ile Lys Gly Asn His Leu Val Lys Val 
            20                  25                  30          


Tyr Asp Tyr Gln Glu Asp Gly Ser Val Leu Leu Thr Cys Asp Ala Glu 
        35                  40                  45              


Ala Lys Asn Ile Thr Trp Phe Lys Asp Gly Lys Met Ile Gly Phe Leu 
    50                  55                  60                  


Thr Glu Asp Lys Lys Lys Trp Asn Leu Gly Ser Asn Ala Lys Asp Pro 
65                  70                  75                  80  


Arg Gly Met Tyr Gln Cys Lys Gly Ser Gln Asn Lys Ser Lys Pro Leu 
                85                  90                  95      


Gln Val Tyr Tyr Arg Met Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
            100                 105                 110         


Gly Gly Gly Gly Ser Asp Gly Asn Glu Glu Met Gly Gly Ile Thr Gln 
        115                 120                 125             


Thr Pro Tyr Lys Val Ser Ile Ser Gly Thr Thr Val Ile Leu Thr Cys 
    130                 135                 140                 


Pro Gln Tyr Pro Gly Ser Glu Ile Leu Trp Gln His Asn Asp Lys Asn 
145                 150                 155                 160 


Ile Gly Gly Asp Glu Asp Asp Lys Asn Ile Gly Ser Asp Glu Asp His 
                165                 170                 175     


Leu Ser Leu Lys Glu Phe Ser Glu Leu Glu Gln Ser Gly Tyr Tyr Val 
            180                 185                 190         


Cys Tyr Pro Arg Gly Ser Lys Pro Glu Asp Ala Asn Phe Tyr Leu Tyr 
        195                 200                 205             


Leu Arg Ala Arg Val Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro 
    210                 215                 220                 


Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys 
225                 230                 235                 240 


Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala 
                245                 250                 255     


Cys Asp Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr 
            260                 265                 270         


Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys 
        275                 280                 285             


Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
    290                 295                 300                 


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
305                 310                 315                 320 


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
                325                 330                 335     


Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
            340                 345                 350         


Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu 
        355                 360                 365             


Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
    370                 375                 380                 


Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro 
385                 390                 395                 400 


Arg Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp 
                405                 410                 415     


Val Glu Glu Asn Pro Gly Pro Met Val Ser Lys Gly Glu Glu Leu Phe 
            420                 425                 430         


Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly 
        435                 440                 445             


His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly 
    450                 455                 460                 


Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val Pro 
465                 470                 475                 480 


Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr Gly Val Gln Cys Phe Ser 
                485                 490                 495     


Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser Ala Met 
            500                 505                 510         


Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly 
        515                 520                 525             


Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val 
    530                 535                 540                 


Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile 
545                 550                 555                 560 


Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr Ile 
                565                 570                 575     


Met Ala Asp Lys Gln Lys Asn Gly Ile Lys Val Asn Phe Lys Ile Arg 
            580                 585                 590         


His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln 
        595                 600                 605             


Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr 
    610                 615                 620                 


Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp 
625                 630                 635                 640 


His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Leu Gly 
                645                 650                 655     


Met Asp Glu Leu Tyr Lys 
            660         


<210>  24
<211>  1989
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD3-Chimera complete sequence nt gamma/epsilon (codon optimized)

<400>  24
atggccctgc ctgtgacagc tctgctgctg cctctggccc tgctgctgca tgccgccaga       60

ccccagtcaa tcaaaggaaa ccacttggtt aaggtgtatg actatcaaga agatggttcg      120

gtacttctga cttgtgatgc agaagccaaa aatatcacat ggtttaaaga tgggaagatg      180

atcggcttcc taactgaaga taaaaaaaaa tggaatctgg gaagtaatgc caaggaccct      240

cgagggatgt atcagtgtaa aggatcacag aacaagtcaa aaccactcca agtgtattac      300

agaatgggcg gaggcggatc tggcggcgga ggatcagggg gagggggctc tgatggcaac      360

gaggaaatgg gcggcatcac ccagaccccc tacaaggtgt cgatatctgg caccaccgtg      420

atcctgacct gcccccagta tcccggcagc gagattctgt ggcagcacaa cgacaagaac      480

atcggcggcg acgaggacga taagaatatc ggctccgatg aggaccacct gagcctgaaa      540

gagttcagcg agctggaaca gagcggctac tacgtgtgct accccagggg atccaagccc      600

gaggacgcca acttctacct gtacctgcgg gccagagtga ccaccacccc tgctcctaga      660

ccacctaccc cagcccctac aatcgccagc cagcctctgt ctctgaggcc cgaggcttgt      720

agaccagctg ctggcggagc cgtgcacacc agaggactgg atttcgcctg cgacttctgg      780

gtgctggtgg tcgtgggcgg cgtgctagct tgttacagcc tgctcgtgac cgtggccttc      840

atcatctttt gggtgcgcag caagcgcgtg aagttcagca gaagcgccga cgcccctgcc      900

taccagcagg gacagaacca gctgtacaac gagctgaacc tgggtcgacg agaggaatac      960

gacgtgctgg acaagcggag aggccgggac cctgagatgg gaggaaagcc ccagcggaga     1020

aagaaccccc aggaaggcct gtataacgaa ctgcagaaag acaagatggc cgaggcctac     1080

agcgagatcg gaatgaaggg cgagcgcaga agaggcaagg gacacgatgg actgtaccag     1140

ggactgagca ccgccaccaa ggatacctat gacgccctgc acatgcaggc ccttccacct     1200

agaggttccg gagccaccaa ctttagcctg ctgaaacagg ccggcgacgt ggaagagaac     1260

cctggaccta tggtgtccaa aggcgaggaa ctgttcaccg gcgtggtgcc cattctggtg     1320

gaactggacg gggatgtgaa cggccacaag tttagcgtta gcggcgaagg cgaaggggat     1380

gccacatacg gaaagctgac cctgaagttc atctgcacca ccggcaagct gcctgtgcct     1440

tggcctacac tggtcaccac actgacatac ggcgtgcagt gctttagcag ataccccgac     1500

catatgaagc agcacgactt cttcaagagc gccatgcctg agggctacgt gcaagagcgg     1560

accatcttct ttaaggacga cggcaactac aagaccagag ccgaagtgaa gtttgagggc     1620

gacaccctgg tcaaccggat cgagctgaag ggcatcgact tcaaagagga cggcaacatc     1680

ctgggccaca agctcgagta caactacaac tcccacaacg tgtacatcat ggccgacaag     1740

cagaaaaacg gcatcaaagt gaacttcaag atccggcaca acatcgagga cggctctgtg     1800

cagctggccg atcactacca gcagaacaca cccatcggag atggccctgt gctgctgccc     1860

gataaccact acctgagcac acagagcgcc ctgtctaagg accccaacga gaagagggac     1920

cacatggtgc tgctcgagtt tgtgacagcc gccggaatca ccctcggcat ggacgagctg     1980

tataagtga                                                             1989


<210>  25
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Control peptide

<400>  25

Ser Leu Leu Met Trp Ile Thr Gln Cys 
1               5                   


