                         SEQUENCE LISTING

<110>  BIO-TECHNE CORPORATION
 
<120>  METHODS OF USING A TFGbeta KNOCKOUT CELL LINE AND COMPOSITIONS 
       RESULTING THEREFROM

<130>  0541.000008WO01

<150>  62/994,004
<151>  2020-03-24

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  400
<212>  DNA
<213>  artificial

<220>
<223>  TGFbeta1 including 64-base pair insert

<400>  1
cccacccacc ccagccaccc cagccctgca gaggcggggc ctccgcatcc cacctttccg       60

cccccccacc ccggggttcg cgctctccgc cgggccgcgc gcgcgagcga gcgagcgcgg      120

ggcgccgcct ctcccatgcc gccctcgggg ctgcggctcc tgccgcttct gctcccgctg      180

ccgtggctcc tggtgctgac gccggggagg ccagccgtgg ggctctccac ctgcaagacc      240

atcgacatgg agggcagaga cctgtttctc tgcttcttgg gaagctgagg caggagaatt      300

tcaagtttta gggcagctgg tgaaacgcaa acgcatcgaa gccatccgtg gccagatcct      360

gtccaaactc cggctcgcca gccccccgag ccagggggag                            400


<210>  2
<211>  400
<212>  DNA
<213>  artificial

<220>
<223>  TGFbeta1 including a 64-base pair insert

<400>  2
ctccccctgg ctcggggggc tggcgagccg gagtttggac aggatctggc cacggatggc       60

ttcgatgcgt ttgcgtttca ccagctgccc taaaacttga aattctcctg cctcagcttc      120

ccaagaagca gagaaacagg tctctgccct ccatgtcgat ggtcttgcag gtggagagcc      180

ccacggctgg cctccccggc gtcagcacca ggagccacgg cagcgggagc agaagcggca      240

ggagccgcag ccccgagggc ggcatgggag aggcggcgcc ccgcgctcgc tcgctcgcgc      300

gcgcggcccg gcggagagcg cgaaccccgg ggtggggggg cggaaaggtg ggatgcggag      360

gccccgcctc tgcagggctg gggtggctgg ggtgggtggg                            400


<210>  3
<211>  88
<212>  PRT
<213>  artificial

<220>
<223>  TGFbeta1 including 64-base pair insert


<220>
<221>  MISC_FEATURE
<222>  (51)..(51)
<223>  stop codon

<400>  3

Met Pro Pro Ser Gly Leu Arg Leu Leu Pro Leu Leu Leu Pro Leu Pro 
1               5                   10                  15      


Trp Leu Leu Val Leu Thr Pro Gly Arg Pro Ala Val Gly Leu Ser Thr 
            20                  25                  30          


Cys Lys Thr Ile Asp Met Glu Gly Arg Asp Leu Phe Leu Cys Phe Leu 
        35                  40                  45              


Gly Ser Xaa Gly Arg Arg Ile Ser Ser Phe Arg Ala Ala Gly Glu Thr 
    50                  55                  60                  


Gln Thr His Arg Ser His Pro Trp Pro Asp Pro Val Gln Thr Pro Ala 
65                  70                  75                  80  


Arg Gln Pro Pro Glu Pro Gly Gly 
                85              


<210>  4
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  4
gctcccctat ttaagaacac                                                   20


<210>  5
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  5
gctctgccgg tggtttcctc                                                   20


<210>  6
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  6
ttcagggctc tctcctaacc a                                                 21


