                         SEQUENCE LISTING

<110>  ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
 
<120>  RECOMBINANT SARS-COV-2 SPIKE PROTEIN AND USES THEREOF

<130>  6923-342-228

<140>
<141>

<150>  63/059,924
<151>  2020-07-31

<150>  63/051,858
<151>  2020-07-14

<150>  63/024,436
<151>  2020-05-13

<150>  63/020,503
<151>  2020-05-05

<150>  63/018,457
<151>  2020-04-30

<150>  62/994,252
<151>  2020-03-24

<160>  10    

<170>  PatentIn version 3.5

<210>  1
<211>  735
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SARS-CoV-2 RBD (codon-optimized)

<400>  1
atgttcgtgt ttctggtgct gctgcctctg gtgtccagcc agcgggtgca gcccaccgaa       60

tccatcgtgc ggttccccaa tatcaccaat ctgtgcccct tcggcgaggt gttcaatgcc      120

accagattcg cctctgtgta cgcctggaac cggaagcgga tcagcaattg cgtggccgac      180

tactccgtgc tgtacaactc cgccagcttc agcaccttca agtgctacgg cgtgtcccct      240

accaagctga acgacctgtg cttcacaaac gtgtacgccg acagcttcgt gatccgggga      300

gatgaagtgc ggcagattgc ccctggacag acaggcaaga tcgccgacta caactacaag      360

ctgcccgacg acttcaccgg ctgtgtgatt gcctggaaca gcaacaacct ggactccaaa      420

gtcggcggca actacaatta cctgtaccgg ctgttccgga agtccaatct gaagcccttc      480

gagcgggaca tctccaccga gatctatcag gccggcagca ccccttgtaa cggcgtggaa      540

ggcttcaact gctacttccc actgcagtcc tacggctttc agcccacaaa tggcgtgggc      600

tatcagccct acagagtggt ggtgctgagc ttcgaactgc tgcatgcccc tgccacagtg      660

tgcggcccta agaaaagcac caatctcgtg aagaacaaat gcgtgaactt ccaccatcac      720

catcaccatt gataa                                                       735


<210>  2
<211>  243
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SARS-CoV-2 RBD

<400>  2

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Arg Val 
1               5                   10                  15      


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
            20                  25                  30          


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
        35                  40                  45              


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
    50                  55                  60                  


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
65                  70                  75                  80  


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
                85                  90                  95      


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
            100                 105                 110         


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
        115                 120                 125             


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
    130                 135                 140                 


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
145                 150                 155                 160 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
                165                 170                 175     


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
            180                 185                 190         


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
        195                 200                 205             


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
    210                 215                 220                 


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe His His His 
225                 230                 235                 240 


His His His 
            


<210>  3
<211>  3774
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SARS-CoV-2 Spike with CS and PP (codon-optimized)

<400>  3
atgttcgtgt ttctggtgct gctgcctctg gtgtccagcc agtgtgtgaa cctgaccaca       60

agaacccagc tgcctccagc ctacaccaac agctttacca gaggcgtgta ctaccccgac      120

aaggtgttca gatccagcgt gctgcactct acccaggacc tgttcctgcc tttcttcagc      180

aacgtgacct ggttccacgc catccacgtg tccggcacca atggcaccaa gagattcgac      240

aaccccgtgc tgcccttcaa cgacggggtg tactttgcca gcaccgagaa gtccaacatc      300

atcagaggct ggatcttcgg caccacactg gacagcaaga cccagagcct gctgatcgtg      360

aacaacgcca ccaacgtggt catcaaagtg tgcgagttcc agttctgcaa cgaccccttc      420

ctgggcgtct actatcacaa gaacaacaag agctggatgg aaagcgagtt ccgggtgtac      480

agcagcgcca acaactgcac cttcgagtac gtgtcccagc ctttcctgat ggacctggaa      540

ggcaagcagg gcaacttcaa gaacctgcgc gagttcgtgt tcaagaacat cgacggctac      600

ttcaagatct acagcaagca cacccctatc aacctcgtgc gggatctgcc tcagggcttc      660

tctgctctgg aacccctggt ggatctgccc atcggcatca acatcacccg gtttcagaca      720

ctgctggccc tgcacagaag ctacctgaca cctggcgata gcagcagcgg atggacagct      780

ggtgccgccg cttactatgt gggctacctg cagcctagaa cctttctgct gaagtacaac      840

gagaacggca ccatcaccga cgccgtggat tgtgctctgg atcctctgag cgagacaaag      900

tgcaccctga agtccttcac cgtggaaaag ggcatctacc agaccagcaa cttccgggtg      960

cagcccaccg aatccatcgt gcggttcccc aatatcacca atctgtgccc cttcggcgag     1020

gtgttcaatg ccaccagatt cgcctctgtg tacgcctgga accggaagcg gatcagcaat     1080

tgcgtggccg actactccgt gctgtacaac tccgccagct tcagcacctt caagtgctac     1140

ggcgtgtccc ctaccaagct gaacgacctg tgcttcacaa acgtgtacgc cgacagcttc     1200

gtgatccggg gagatgaagt gcggcagatt gcccctggac agacaggcaa gatcgccgac     1260

tacaactaca agctgcccga cgacttcacc ggctgtgtga ttgcctggaa cagcaacaac     1320

ctggactcca aagtcggcgg caactacaat tacctgtacc ggctgttccg gaagtccaat     1380

ctgaagccct tcgagcggga catctccacc gagatctatc aggccggcag caccccttgt     1440

aacggcgtgg aaggcttcaa ctgctacttc ccactgcagt cctacggctt tcagcccaca     1500

aatggcgtgg gctatcagcc ctacagagtg gtggtgctga gcttcgaact gctgcatgcc     1560

cctgccacag tgtgcggccc taagaaaagc accaatctcg tgaagaacaa atgcgtgaac     1620

ttcaacttca acggcctgac cggcaccggc gtgctgacag agagcaacaa gaagttcctg     1680

ccattccagc agtttggccg ggatatcgcc gataccacag acgccgttag agatccccag     1740

acactggaaa tcctggacat caccccttgc agcttcggcg gagtgtctgt gatcacccct     1800

ggcaccaaca ccagcaatca ggtggcagtg ctgtaccagg acgtgaactg taccgaagtg     1860

cccgtggcca ttcacgccga tcagctgaca cctacatggc gggtgtactc caccggcagc     1920

aatgtgtttc agaccagagc cggctgtctg atcggagccg agcacgtgaa caatagctac     1980

gagtgcgaca tccccatcgg cgctggcatc tgtgccagct accagacaca gacaaacagc     2040

cccgcctctg tggccagcca gagcatcatt gcctacacaa tgtctctggg cgccgagaac     2100

agcgtggcct actccaacaa ctctatcgct atccccacca acttcaccat cagcgtgacc     2160

acagagatcc tgcctgtgtc catgaccaag accagcgtgg actgcaccat gtacatctgc     2220

ggcgattcca ccgagtgctc caacctgctg ctgcagtacg gcagcttctg cacccagctg     2280

aatagagccc tgacagggat cgccgtggaa caggacaaga acacccaaga ggtgttcgcc     2340

caagtgaagc agatctacaa gacccctcct atcaaggact tcggcggctt caatttcagc     2400

cagattctgc ccgatcctag caagcccagc aagcggagct tcatcgagga cctgctgttc     2460

aacaaagtga cactggccga cgccggcttc atcaagcagt atggcgattg tctgggcgac     2520

attgccgcca gggatctgat ttgcgcccag aagtttaacg gactgacagt gctgcctcct     2580

ctgctgaccg atgagatgat cgcccagtac acatctgccc tgctggccgg cacaatcaca     2640

agcggctgga catttggagc tggcgccgct ctgcagatcc cctttgctat gcagatggcc     2700

taccggttca acggcatcgg agtgacccag aatgtgctgt acgagaacca gaagctgatc     2760

gccaaccagt tcaacagcgc catcggcaag atccaggaca gcctgagcag cacagcaagc     2820

gccctgggaa agctgcagga cgtggtcaac cagaatgccc aggcactgaa caccctggtc     2880

aagcagctgt cctccaactt cggcgccatc agctctgtgc tgaacgatat cctgagcaga     2940

ctggaccctc ctgaagccga ggtgcagatc gacagactga tcaccggaag gctgcagtcc     3000

ctgcagacct acgttaccca gcagctgatc agagccgccg agattagagc ctctgccaat     3060

ctggccgcca ccaagatgtc tgagtgtgtg ctgggccaga gcaagagagt ggacttttgc     3120

ggcaagggct accacctgat gagcttccct cagtctgccc ctcacggcgt ggtgtttctg     3180

cacgtgacat acgtgcccgc tcaagagaag aatttcacca ccgctccagc catctgccac     3240

gacggcaaag cccactttcc tagagaaggc gtgttcgtgt ccaacggcac ccattggttc     3300

gtgacccagc ggaacttcta cgagccccag atcatcacca ccgacaacac cttcgtgtct     3360

ggcaactgcg acgtcgtgat cggcattgtg aacaataccg tgtacgaccc tctgcagccc     3420

gagctggaca gcttcaaaga ggaactggat aagtacttta agaaccacac aagccccgac     3480

gtggacctgg gcgatatcag cggaatcaat gccagcgtcg tgaacatcca gaaagagatc     3540

gaccggctga acgaggtggc caagaatctg aacgagagcc tgatcgacct gcaagaactg     3600

gggaagtacg agcagtacat caagtggccc agcggccgct tggtcccacg tggctcaccc     3660

ggatctggat acatcccgga ggcccctagg gacggtcaag cttacgtgag aaaggacggc     3720

gaatgggttc tgctgtcgac cttcttggga catcatcatc atcatcacta atga           3774


<210>  4
<211>  1256
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SARS-CoV-2 Spike with CS and PP

<400>  4

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Ala Ser Val Ala Ser Gln Ser 
        675                 680                 685             


Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser Val Ala Tyr 
    690                 695                 700                 


Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile Ser Val Thr 
705                 710                 715                 720 


Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val Asp Cys Thr 
                725                 730                 735     


Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu Leu Leu Gln 
            740                 745                 750         


Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr Gly Ile Ala 
        755                 760                 765             


Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln Val Lys Gln 
    770                 775                 780                 


Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe Asn Phe Ser 
785                 790                 795                 800 


Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser Phe Ile Glu 
                805                 810                 815     


Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly Phe Ile Lys 
            820                 825                 830         


Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp Leu Ile Cys 
        835                 840                 845             


Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu Leu Thr Asp 
    850                 855                 860                 


Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly Thr Ile Thr 
865                 870                 875                 880 


Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro Phe Ala 
                885                 890                 895     


Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln Asn Val 
            900                 905                 910         


Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn Ser Ala Ile 
        915                 920                 925             


Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala Leu Gly Lys 
    930                 935                 940                 


Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn Thr Leu Val 
945                 950                 955                 960 


Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val Leu Asn Asp 
                965                 970                 975     


Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln Ile Asp Arg 
            980                 985                 990         


Leu Ile Thr Gly Arg Leu Gln Ser  Leu Gln Thr Tyr Val  Thr Gln Gln 
        995                 1000                 1005             


Leu Ile  Arg Ala Ala Glu Ile  Arg Ala Ser Ala Asn  Leu Ala Ala 
    1010                 1015                 1020             


Thr Lys  Met Ser Glu Cys Val  Leu Gly Gln Ser Lys  Arg Val Asp 
    1025                 1030                 1035             


Phe Cys  Gly Lys Gly Tyr His  Leu Met Ser Phe Pro  Gln Ser Ala 
    1040                 1045                 1050             


Pro His  Gly Val Val Phe Leu  His Val Thr Tyr Val  Pro Ala Gln 
    1055                 1060                 1065             


Glu Lys  Asn Phe Thr Thr Ala  Pro Ala Ile Cys His  Asp Gly Lys 
    1070                 1075                 1080             


Ala His  Phe Pro Arg Glu Gly  Val Phe Val Ser Asn  Gly Thr His 
    1085                 1090                 1095             


Trp Phe  Val Thr Gln Arg Asn  Phe Tyr Glu Pro Gln  Ile Ile Thr 
    1100                 1105                 1110             


Thr Asp  Asn Thr Phe Val Ser  Gly Asn Cys Asp Val  Val Ile Gly 
    1115                 1120                 1125             


Ile Val  Asn Asn Thr Val Tyr  Asp Pro Leu Gln Pro  Glu Leu Asp 
    1130                 1135                 1140             


Ser Phe  Lys Glu Glu Leu Asp  Lys Tyr Phe Lys Asn  His Thr Ser 
    1145                 1150                 1155             


Pro Asp  Val Asp Leu Gly Asp  Ile Ser Gly Ile Asn  Ala Ser Val 
    1160                 1165                 1170             


Val Asn  Ile Gln Lys Glu Ile  Asp Arg Leu Asn Glu  Val Ala Lys 
    1175                 1180                 1185             


Asn Leu  Asn Glu Ser Leu Ile  Asp Leu Gln Glu Leu  Gly Lys Tyr 
    1190                 1195                 1200             


Glu Gln  Tyr Ile Lys Trp Pro  Ser Gly Arg Leu Val  Pro Arg Gly 
    1205                 1210                 1215             


Ser Pro  Gly Ser Gly Tyr Ile  Pro Glu Ala Pro Arg  Asp Gly Gln 
    1220                 1225                 1230             


Ala Tyr  Val Arg Lys Asp Gly  Glu Trp Val Leu Leu  Ser Thr Phe 
    1235                 1240                 1245             


Leu Gly  His His His His His  His 
    1250                 1255     


<210>  5
<211>  3774
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SARS-CoV-2 Spike with CS (codon-optimized)

<400>  5
atgttcgtgt ttctggtgct gctgcctctg gtgtccagcc agtgtgtgaa cctgaccaca       60

agaacccagc tgcctccagc ctacaccaac agctttacca gaggcgtgta ctaccccgac      120

aaggtgttca gatccagcgt gctgcactct acccaggacc tgttcctgcc tttcttcagc      180

aacgtgacct ggttccacgc catccacgtg tccggcacca atggcaccaa gagattcgac      240

aaccccgtgc tgcccttcaa cgacggggtg tactttgcca gcaccgagaa gtccaacatc      300

atcagaggct ggatcttcgg caccacactg gacagcaaga cccagagcct gctgatcgtg      360

aacaacgcca ccaacgtggt catcaaagtg tgcgagttcc agttctgcaa cgaccccttc      420

ctgggcgtct actatcacaa gaacaacaag agctggatgg aaagcgagtt ccgggtgtac      480

agcagcgcca acaactgcac cttcgagtac gtgtcccagc ctttcctgat ggacctggaa      540

ggcaagcagg gcaacttcaa gaacctgcgc gagttcgtgt tcaagaacat cgacggctac      600

ttcaagatct acagcaagca cacccctatc aacctcgtgc gggatctgcc tcagggcttc      660

tctgctctgg aacccctggt ggatctgccc atcggcatca acatcacccg gtttcagaca      720

ctgctggccc tgcacagaag ctacctgaca cctggcgata gcagcagcgg atggacagct      780

ggtgccgccg cttactatgt gggctacctg cagcctagaa cctttctgct gaagtacaac      840

gagaacggca ccatcaccga cgccgtggat tgtgctctgg atcctctgag cgagacaaag      900

tgcaccctga agtccttcac cgtggaaaag ggcatctacc agaccagcaa cttccgggtg      960

cagcccaccg aatccatcgt gcggttcccc aatatcacca atctgtgccc cttcggcgag     1020

gtgttcaatg ccaccagatt cgcctctgtg tacgcctgga accggaagcg gatcagcaat     1080

tgcgtggccg actactccgt gctgtacaac tccgccagct tcagcacctt caagtgctac     1140

ggcgtgtccc ctaccaagct gaacgacctg tgcttcacaa acgtgtacgc cgacagcttc     1200

gtgatccggg gagatgaagt gcggcagatt gcccctggac agacaggcaa gatcgccgac     1260

tacaactaca agctgcccga cgacttcacc ggctgtgtga ttgcctggaa cagcaacaac     1320

ctggactcca aagtcggcgg caactacaat tacctgtacc ggctgttccg gaagtccaat     1380

ctgaagccct tcgagcggga catctccacc gagatctatc aggccggcag caccccttgt     1440

aacggcgtgg aaggcttcaa ctgctacttc ccactgcagt cctacggctt tcagcccaca     1500

aatggcgtgg gctatcagcc ctacagagtg gtggtgctga gcttcgaact gctgcatgcc     1560

cctgccacag tgtgcggccc taagaaaagc accaatctcg tgaagaacaa atgcgtgaac     1620

ttcaacttca acggcctgac cggcaccggc gtgctgacag agagcaacaa gaagttcctg     1680

ccattccagc agtttggccg ggatatcgcc gataccacag acgccgttag agatccccag     1740

acactggaaa tcctggacat caccccttgc agcttcggcg gagtgtctgt gatcacccct     1800

ggcaccaaca ccagcaatca ggtggcagtg ctgtaccagg acgtgaactg taccgaagtg     1860

cccgtggcca ttcacgccga tcagctgaca cctacatggc gggtgtactc caccggcagc     1920

aatgtgtttc agaccagagc cggctgtctg atcggagccg agcacgtgaa caatagctac     1980

gagtgcgaca tccccatcgg cgctggcatc tgtgccagct accagacaca gacaaacagc     2040

cccgcctctg tggccagcca gagcatcatt gcctacacaa tgtctctggg cgccgagaac     2100

agcgtggcct actccaacaa ctctatcgct atccccacca acttcaccat cagcgtgacc     2160

acagagatcc tgcctgtgtc catgaccaag accagcgtgg actgcaccat gtacatctgc     2220

ggcgattcca ccgagtgctc caacctgctg ctgcagtacg gcagcttctg cacccagctg     2280

aatagagccc tgacagggat cgccgtggaa caggacaaga acacccaaga ggtgttcgcc     2340

caagtgaagc agatctacaa gacccctcct atcaaggact tcggcggctt caatttcagc     2400

cagattctgc ccgatcctag caagcccagc aagcggagct tcatcgagga cctgctgttc     2460

aacaaagtga cactggccga cgccggcttc atcaagcagt atggcgattg tctgggcgac     2520

attgccgcca gggatctgat ttgcgcccag aagtttaacg gactgacagt gctgcctcct     2580

ctgctgaccg atgagatgat cgcccagtac acatctgccc tgctggccgg cacaatcaca     2640

agcggctgga catttggagc tggcgccgct ctgcagatcc cctttgctat gcagatggcc     2700

taccggttca acggcatcgg agtgacccag aatgtgctgt acgagaacca gaagctgatc     2760

gccaaccagt tcaacagcgc catcggcaag atccaggaca gcctgagcag cacagcaagc     2820

gccctgggaa agctgcagga cgtggtcaac cagaatgccc aggcactgaa caccctggtc     2880

aagcagctgt cctccaactt cggcgccatc agctctgtgc tgaacgatat cctgagcaga     2940

ctggacaagg tggaagccga ggtgcagatc gacagactga tcaccggaag gctgcagtcc     3000

ctgcagacct acgttaccca gcagctgatc agagccgccg agattagagc ctctgccaat     3060

ctggccgcca ccaagatgtc tgagtgtgtg ctgggccaga gcaagagagt ggacttttgc     3120

ggcaagggct accacctgat gagcttccct cagtctgccc ctcacggcgt ggtgtttctg     3180

cacgtgacat acgtgcccgc tcaagagaag aatttcacca ccgctccagc catctgccac     3240

gacggcaaag cccactttcc tagagaaggc gtgttcgtgt ccaacggcac ccattggttc     3300

gtgacccagc ggaacttcta cgagccccag atcatcacca ccgacaacac cttcgtgtct     3360

ggcaactgcg acgtcgtgat cggcattgtg aacaataccg tgtacgaccc tctgcagccc     3420

gagctggaca gcttcaaaga ggaactggat aagtacttta agaaccacac aagccccgac     3480

gtggacctgg gcgatatcag cggaatcaat gccagcgtcg tgaacatcca gaaagagatc     3540

gaccggctga acgaggtggc caagaatctg aacgagagcc tgatcgacct gcaagaactg     3600

gggaagtacg agcagtacat caagtggccc agcggccgct tggtcccacg tggctcaccc     3660

ggatctggat acatcccgga ggcccctagg gacggtcaag cttacgtgag aaaggacggc     3720

gaatgggttc tgctgtcgac cttcttggga catcatcatc atcatcacta atga           3774


<210>  6
<211>  1256
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SARS-CoV-2 Spike with CS

<400>  6

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Ala Ser Val Ala Ser Gln Ser 
        675                 680                 685             


Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser Val Ala Tyr 
    690                 695                 700                 


Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile Ser Val Thr 
705                 710                 715                 720 


Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val Asp Cys Thr 
                725                 730                 735     


Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu Leu Leu Gln 
            740                 745                 750         


Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr Gly Ile Ala 
        755                 760                 765             


Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln Val Lys Gln 
    770                 775                 780                 


Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe Asn Phe Ser 
785                 790                 795                 800 


Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser Phe Ile Glu 
                805                 810                 815     


Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly Phe Ile Lys 
            820                 825                 830         


Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp Leu Ile Cys 
        835                 840                 845             


Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu Leu Thr Asp 
    850                 855                 860                 


Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly Thr Ile Thr 
865                 870                 875                 880 


Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro Phe Ala 
                885                 890                 895     


Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln Asn Val 
            900                 905                 910         


Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn Ser Ala Ile 
        915                 920                 925             


Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala Leu Gly Lys 
    930                 935                 940                 


Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn Thr Leu Val 
945                 950                 955                 960 


Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val Leu Asn Asp 
                965                 970                 975     


Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln Ile Asp Arg 
            980                 985                 990         


Leu Ile Thr Gly Arg Leu Gln Ser  Leu Gln Thr Tyr Val  Thr Gln Gln 
        995                 1000                 1005             


Leu Ile  Arg Ala Ala Glu Ile  Arg Ala Ser Ala Asn  Leu Ala Ala 
    1010                 1015                 1020             


Thr Lys  Met Ser Glu Cys Val  Leu Gly Gln Ser Lys  Arg Val Asp 
    1025                 1030                 1035             


Phe Cys  Gly Lys Gly Tyr His  Leu Met Ser Phe Pro  Gln Ser Ala 
    1040                 1045                 1050             


Pro His  Gly Val Val Phe Leu  His Val Thr Tyr Val  Pro Ala Gln 
    1055                 1060                 1065             


Glu Lys  Asn Phe Thr Thr Ala  Pro Ala Ile Cys His  Asp Gly Lys 
    1070                 1075                 1080             


Ala His  Phe Pro Arg Glu Gly  Val Phe Val Ser Asn  Gly Thr His 
    1085                 1090                 1095             


Trp Phe  Val Thr Gln Arg Asn  Phe Tyr Glu Pro Gln  Ile Ile Thr 
    1100                 1105                 1110             


Thr Asp  Asn Thr Phe Val Ser  Gly Asn Cys Asp Val  Val Ile Gly 
    1115                 1120                 1125             


Ile Val  Asn Asn Thr Val Tyr  Asp Pro Leu Gln Pro  Glu Leu Asp 
    1130                 1135                 1140             


Ser Phe  Lys Glu Glu Leu Asp  Lys Tyr Phe Lys Asn  His Thr Ser 
    1145                 1150                 1155             


Pro Asp  Val Asp Leu Gly Asp  Ile Ser Gly Ile Asn  Ala Ser Val 
    1160                 1165                 1170             


Val Asn  Ile Gln Lys Glu Ile  Asp Arg Leu Asn Glu  Val Ala Lys 
    1175                 1180                 1185             


Asn Leu  Asn Glu Ser Leu Ile  Asp Leu Gln Glu Leu  Gly Lys Tyr 
    1190                 1195                 1200             


Glu Gln  Tyr Ile Lys Trp Pro  Ser Gly Arg Leu Val  Pro Arg Gly 
    1205                 1210                 1215             


Ser Pro  Gly Ser Gly Tyr Ile  Pro Glu Ala Pro Arg  Asp Gly Gln 
    1220                 1225                 1230             


Ala Tyr  Val Arg Lys Asp Gly  Glu Trp Val Leu Leu  Ser Thr Phe 
    1235                 1240                 1245             


Leu Gly  His His His His His  His 
    1250                 1255     


<210>  7
<211>  8529
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid Sequence

<400>  7
gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata       60

gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc      120

ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag      180

ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac      240

atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg      300

cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg      360

tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc      420

atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca      480

gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg      540

gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt      600

tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc      660

gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc      720

ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc      780

gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag      840

ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg      900

tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg      960

cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc     1020

ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg     1080

tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc     1140

cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc     1200

gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg     1260

ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct     1320

gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg     1380

gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc     1440

tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt     1500

cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg     1560

acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg     1620

gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca     1680

acgtgctggt tgttgtgctg tctcatcatt ttggcaaagg ccaccatgtt cgtgtttctg     1740

gtgctgctgc ctctggtgtc cagccagtgt gtgaacctga ccacaagaac ccagctgcct     1800

ccagcctaca ccaacagctt taccagaggc gtgtactacc ccgacaaggt gttcagatcc     1860

agcgtgctgc actctaccca ggacctgttc ctgcctttct tcagcaacgt gacctggttc     1920

cacgccatcc acgtgtccgg caccaatggc accaagagat tcgacaaccc cgtgctgccc     1980

ttcaacgacg gggtgtactt tgccagcacc gagaagtcca acatcatcag aggctggatc     2040

ttcggcacca cactggacag caagacccag agcctgctga tcgtgaacaa cgccaccaac     2100

gtggtcatca aagtgtgcga gttccagttc tgcaacgacc ccttcctggg cgtctactat     2160

cacaagaaca acaagagctg gatggaaagc gagttccggg tgtacagcag cgccaacaac     2220

tgcaccttcg agtacgtgtc ccagcctttc ctgatggacc tggaaggcaa gcagggcaac     2280

ttcaagaacc tgcgcgagtt cgtgttcaag aacatcgacg gctacttcaa gatctacagc     2340

aagcacaccc ctatcaacct cgtgcgggat ctgcctcagg gcttctctgc tctggaaccc     2400

ctggtggatc tgcccatcgg catcaacatc acccggtttc agacactgct ggccctgcac     2460

agaagctacc tgacacctgg cgatagcagc agcggatgga cagctggtgc cgccgcttac     2520

tatgtgggct acctgcagcc tagaaccttt ctgctgaagt acaacgagaa cggcaccatc     2580

accgacgccg tggattgtgc tctggatcct ctgagcgaga caaagtgcac cctgaagtcc     2640

ttcaccgtgg aaaagggcat ctaccagacc agcaacttcc gggtgcagcc caccgaatcc     2700

atcgtgcggt tccccaatat caccaatctg tgccccttcg gcgaggtgtt caatgccacc     2760

agattcgcct ctgtgtacgc ctggaaccgg aagcggatca gcaattgcgt ggccgactac     2820

tccgtgctgt acaactccgc cagcttcagc accttcaagt gctacggcgt gtcccctacc     2880

aagctgaacg acctgtgctt cacaaacgtg tacgccgaca gcttcgtgat ccggggagat     2940

gaagtgcggc agattgcccc tggacagaca ggcaagatcg ccgactacaa ctacaagctg     3000

cccgacgact tcaccggctg tgtgattgcc tggaacagca acaacctgga ctccaaagtc     3060

ggcggcaact acaattacct gtaccggctg ttccggaagt ccaatctgaa gcccttcgag     3120

cgggacatct ccaccgagat ctatcaggcc ggcagcaccc cttgtaacgg cgtggaaggc     3180

ttcaactgct acttcccact gcagtcctac ggctttcagc ccacaaatgg cgtgggctat     3240

cagccctaca gagtggtggt gctgagcttc gaactgctgc atgcccctgc cacagtgtgc     3300

ggccctaaga aaagcaccaa tctcgtgaag aacaaatgcg tgaacttcaa cttcaacggc     3360

ctgaccggca ccggcgtgct gacagagagc aacaagaagt tcctgccatt ccagcagttt     3420

ggccgggata tcgccgatac cacagacgcc gttagagatc cccagacact ggaaatcctg     3480

gacatcaccc cttgcagctt cggcggagtg tctgtgatca cccctggcac caacaccagc     3540

aatcaggtgg cagtgctgta ccaggacgtg aactgtaccg aagtgcccgt ggccattcac     3600

gccgatcagc tgacacctac atggcgggtg tactccaccg gcagcaatgt gtttcagacc     3660

agagccggct gtctgatcgg agccgagcac gtgaacaata gctacgagtg cgacatcccc     3720

atcggcgctg gcatctgtgc cagctaccag acacagacaa acagccccgc ctctgtggcc     3780

agccagagca tcattgccta cacaatgtct ctgggcgccg agaacagcgt ggcctactcc     3840

aacaactcta tcgctatccc caccaacttc accatcagcg tgaccacaga gatcctgcct     3900

gtgtccatga ccaagaccag cgtggactgc accatgtaca tctgcggcga ttccaccgag     3960

tgctccaacc tgctgctgca gtacggcagc ttctgcaccc agctgaatag agccctgaca     4020

gggatcgccg tggaacagga caagaacacc caagaggtgt tcgcccaagt gaagcagatc     4080

tacaagaccc ctcctatcaa ggacttcggc ggcttcaatt tcagccagat tctgcccgat     4140

cctagcaagc ccagcaagcg gagcttcatc gaggacctgc tgttcaacaa agtgacactg     4200

gccgacgccg gcttcatcaa gcagtatggc gattgtctgg gcgacattgc cgccagggat     4260

ctgatttgcg cccagaagtt taacggactg acagtgctgc ctcctctgct gaccgatgag     4320

atgatcgccc agtacacatc tgccctgctg gccggcacaa tcacaagcgg ctggacattt     4380

ggagctggcg ccgctctgca gatccccttt gctatgcaga tggcctaccg gttcaacggc     4440

atcggagtga cccagaatgt gctgtacgag aaccagaagc tgatcgccaa ccagttcaac     4500

agcgccatcg gcaagatcca ggacagcctg agcagcacag caagcgccct gggaaagctg     4560

caggacgtgg tcaaccagaa tgcccaggca ctgaacaccc tggtcaagca gctgtcctcc     4620

aacttcggcg ccatcagctc tgtgctgaac gatatcctga gcagactgga ccctcctgaa     4680

gccgaggtgc agatcgacag actgatcacc ggaaggctgc agtccctgca gacctacgtt     4740

acccagcagc tgatcagagc cgccgagatt agagcctctg ccaatctggc cgccaccaag     4800

atgtctgagt gtgtgctggg ccagagcaag agagtggact tttgcggcaa gggctaccac     4860

ctgatgagct tccctcagtc tgcccctcac ggcgtggtgt ttctgcacgt gacatacgtg     4920

cccgctcaag agaagaattt caccaccgct ccagccatct gccacgacgg caaagcccac     4980

tttcctagag aaggcgtgtt cgtgtccaac ggcacccatt ggttcgtgac ccagcggaac     5040

ttctacgagc cccagatcat caccaccgac aacaccttcg tgtctggcaa ctgcgacgtc     5100

gtgatcggca ttgtgaacaa taccgtgtac gaccctctgc agcccgagct ggacagcttc     5160

aaagaggaac tggataagta ctttaagaac cacacaagcc ccgacgtgga cctgggcgat     5220

atcagcggaa tcaatgccag cgtcgtgaac atccagaaag agatcgaccg gctgaacgag     5280

gtggccaaga atctgaacga gagcctgatc gacctgcaag aactggggaa gtacgagcag     5340

tacatcaagt ggcccagcgg ccgcttggtc ccacgtggct cacccggatc tggatacatc     5400

ccggaggccc ctagggacgg tcaagcttac gtgagaaagg acggcgaatg ggttctgctg     5460

tcgaccttct tgggacatca tcatcatcat cactaatgaa attcgagctc gcggccgcat     5520

cgatcttaag tcgcgactcg agctagcaga tctttttccc tctgccaaaa attatgggga     5580

catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc     5640

aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca     5700

tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc     5760

tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt     5820

ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt     5880

tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac tagccagatt     5940

tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat gaagatccct     6000

cgacctgcag cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt     6060

atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg     6120

cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg     6180

gaaacctgtc gtgccagcgg atccgcatct caattagtca gcaaccatag tcccgcccct     6240

aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg     6300

actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa     6360

gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt gtttattgca     6420

gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt     6480

tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc     6540

cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt     6600

ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag     6660

ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca     6720

tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt     6780

tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc     6840

gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct     6900

ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg     6960

tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca     7020

agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact     7080

atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta     7140

acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta     7200

actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct     7260

tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt     7320

tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga     7380

tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca     7440

tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat     7500

caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg     7560

cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt     7620

agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag     7680

acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc     7740

gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag     7800

ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca     7860

tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa     7920

ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga     7980

tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata     8040

attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca     8100

agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg     8160

ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg     8220

ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg     8280

cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag     8340

gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac     8400

tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca     8460

tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag     8520

tgccacctg                                                             8529


<210>  8
<211>  5490
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid Sequence

<400>  8
gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata       60

gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc      120

ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag      180

ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac      240

atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg      300

cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg      360

tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc      420

atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca      480

gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg      540

gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt      600

tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc      660

gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc      720

ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc      780

gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag      840

ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg      900

tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg      960

cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc     1020

ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg     1080

tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc     1140

cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc     1200

gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg     1260

ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct     1320

gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg     1380

gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc     1440

tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt     1500

cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg     1560

acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg     1620

gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca     1680

acgtgctggt tgttgtgctg tctcatcatt ttggcaaagg ccaccatgtt cgtgtttctg     1740

gtgctgctgc ctctggtgtc cagccagcgg gtgcagccca ccgaatccat cgtgcggttc     1800

cccaatatca ccaatctgtg ccccttcggc gaggtgttca atgccaccag attcgcctct     1860

gtgtacgcct ggaaccggaa gcggatcagc aattgcgtgg ccgactactc cgtgctgtac     1920

aactccgcca gcttcagcac cttcaagtgc tacggcgtgt cccctaccaa gctgaacgac     1980

ctgtgcttca caaacgtgta cgccgacagc ttcgtgatcc ggggagatga agtgcggcag     2040

attgcccctg gacagacagg caagatcgcc gactacaact acaagctgcc cgacgacttc     2100

accggctgtg tgattgcctg gaacagcaac aacctggact ccaaagtcgg cggcaactac     2160

aattacctgt accggctgtt ccggaagtcc aatctgaagc ccttcgagcg ggacatctcc     2220

accgagatct atcaggccgg cagcacccct tgtaacggcg tggaaggctt caactgctac     2280

ttcccactgc agtcctacgg ctttcagccc acaaatggcg tgggctatca gccctacaga     2340

gtggtggtgc tgagcttcga actgctgcat gcccctgcca cagtgtgcgg ccctaagaaa     2400

agcaccaatc tcgtgaagaa caaatgcgtg aacttccacc atcaccatca ccattgataa     2460

aattcgagct cgcggccgca tcgatcttaa gtcgcgactc gagctagcag atctttttcc     2520

ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata     2580

aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag     2640

gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc     2700

aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta     2760

tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta     2820

gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt     2880

acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc     2940

tcttctctta tgaagatccc tcgacctgca gcccaagctt ggcgtaatca tggtcatagc     3000

tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca     3060

taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct     3120

cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc     3180

agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc     3240

ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc     3300

ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa     3360

aaagctaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat     3420

ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat     3480

gtatcttatc atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg     3540

gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc     3600

ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag     3660

gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa     3720

aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc     3780

gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc     3840

ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg     3900

cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt     3960

cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc     4020

gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc     4080

cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag     4140

agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg     4200

ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa     4260

ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag     4320

gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact     4380

cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa     4440

attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt     4500

accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag     4560

ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca     4620

gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc     4680

agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt     4740

ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg     4800

ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca     4860

gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg     4920

ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca     4980

tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg     5040

tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct     5100

cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca     5160

tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca     5220

gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg     5280

tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac     5340

ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt     5400

attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc     5460

cgcgcacatt tccccgaaaa gtgccacctg                                      5490


<210>  9
<211>  8529
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid Sequence

<400>  9
gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata       60

gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc      120

ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag      180

ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac      240

atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg      300

cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg      360

tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc      420

atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca      480

gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg      540

gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt      600

tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc      660

gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc      720

ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc      780

gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag      840

ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg      900

tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg      960

cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc     1020

ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg     1080

tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc     1140

cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc     1200

gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg     1260

ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct     1320

gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg     1380

gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc     1440

tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt     1500

cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg     1560

acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg     1620

gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca     1680

acgtgctggt tgttgtgctg tctcatcatt ttggcaaagg ccaccatgtt cgtgtttctg     1740

gtgctgctgc ctctggtgtc cagccagtgt gtgaacctga ccacaagaac ccagctgcct     1800

ccagcctaca ccaacagctt taccagaggc gtgtactacc ccgacaaggt gttcagatcc     1860

agcgtgctgc actctaccca ggacctgttc ctgcctttct tcagcaacgt gacctggttc     1920

cacgccatcc acgtgtccgg caccaatggc accaagagat tcgacaaccc cgtgctgccc     1980

ttcaacgacg gggtgtactt tgccagcacc gagaagtcca acatcatcag aggctggatc     2040

ttcggcacca cactggacag caagacccag agcctgctga tcgtgaacaa cgccaccaac     2100

gtggtcatca aagtgtgcga gttccagttc tgcaacgacc ccttcctggg cgtctactat     2160

cacaagaaca acaagagctg gatggaaagc gagttccggg tgtacagcag cgccaacaac     2220

tgcaccttcg agtacgtgtc ccagcctttc ctgatggacc tggaaggcaa gcagggcaac     2280

ttcaagaacc tgcgcgagtt cgtgttcaag aacatcgacg gctacttcaa gatctacagc     2340

aagcacaccc ctatcaacct cgtgcgggat ctgcctcagg gcttctctgc tctggaaccc     2400

ctggtggatc tgcccatcgg catcaacatc acccggtttc agacactgct ggccctgcac     2460

agaagctacc tgacacctgg cgatagcagc agcggatgga cagctggtgc cgccgcttac     2520

tatgtgggct acctgcagcc tagaaccttt ctgctgaagt acaacgagaa cggcaccatc     2580

accgacgccg tggattgtgc tctggatcct ctgagcgaga caaagtgcac cctgaagtcc     2640

ttcaccgtgg aaaagggcat ctaccagacc agcaacttcc gggtgcagcc caccgaatcc     2700

atcgtgcggt tccccaatat caccaatctg tgccccttcg gcgaggtgtt caatgccacc     2760

agattcgcct ctgtgtacgc ctggaaccgg aagcggatca gcaattgcgt ggccgactac     2820

tccgtgctgt acaactccgc cagcttcagc accttcaagt gctacggcgt gtcccctacc     2880

aagctgaacg acctgtgctt cacaaacgtg tacgccgaca gcttcgtgat ccggggagat     2940

gaagtgcggc agattgcccc tggacagaca ggcaagatcg ccgactacaa ctacaagctg     3000

cccgacgact tcaccggctg tgtgattgcc tggaacagca acaacctgga ctccaaagtc     3060

ggcggcaact acaattacct gtaccggctg ttccggaagt ccaatctgaa gcccttcgag     3120

cgggacatct ccaccgagat ctatcaggcc ggcagcaccc cttgtaacgg cgtggaaggc     3180

ttcaactgct acttcccact gcagtcctac ggctttcagc ccacaaatgg cgtgggctat     3240

cagccctaca gagtggtggt gctgagcttc gaactgctgc atgcccctgc cacagtgtgc     3300

ggccctaaga aaagcaccaa tctcgtgaag aacaaatgcg tgaacttcaa cttcaacggc     3360

ctgaccggca ccggcgtgct gacagagagc aacaagaagt tcctgccatt ccagcagttt     3420

ggccgggata tcgccgatac cacagacgcc gttagagatc cccagacact ggaaatcctg     3480

gacatcaccc cttgcagctt cggcggagtg tctgtgatca cccctggcac caacaccagc     3540

aatcaggtgg cagtgctgta ccaggacgtg aactgtaccg aagtgcccgt ggccattcac     3600

gccgatcagc tgacacctac atggcgggtg tactccaccg gcagcaatgt gtttcagacc     3660

agagccggct gtctgatcgg agccgagcac gtgaacaata gctacgagtg cgacatcccc     3720

atcggcgctg gcatctgtgc cagctaccag acacagacaa acagccccgc ctctgtggcc     3780

agccagagca tcattgccta cacaatgtct ctgggcgccg agaacagcgt ggcctactcc     3840

aacaactcta tcgctatccc caccaacttc accatcagcg tgaccacaga gatcctgcct     3900

gtgtccatga ccaagaccag cgtggactgc accatgtaca tctgcggcga ttccaccgag     3960

tgctccaacc tgctgctgca gtacggcagc ttctgcaccc agctgaatag agccctgaca     4020

gggatcgccg tggaacagga caagaacacc caagaggtgt tcgcccaagt gaagcagatc     4080

tacaagaccc ctcctatcaa ggacttcggc ggcttcaatt tcagccagat tctgcccgat     4140

cctagcaagc ccagcaagcg gagcttcatc gaggacctgc tgttcaacaa agtgacactg     4200

gccgacgccg gcttcatcaa gcagtatggc gattgtctgg gcgacattgc cgccagggat     4260

ctgatttgcg cccagaagtt taacggactg acagtgctgc ctcctctgct gaccgatgag     4320

atgatcgccc agtacacatc tgccctgctg gccggcacaa tcacaagcgg ctggacattt     4380

ggagctggcg ccgctctgca gatccccttt gctatgcaga tggcctaccg gttcaacggc     4440

atcggagtga cccagaatgt gctgtacgag aaccagaagc tgatcgccaa ccagttcaac     4500

agcgccatcg gcaagatcca ggacagcctg agcagcacag caagcgccct gggaaagctg     4560

caggacgtgg tcaaccagaa tgcccaggca ctgaacaccc tggtcaagca gctgtcctcc     4620

aacttcggcg ccatcagctc tgtgctgaac gatatcctga gcagactgga caaggtggaa     4680

gccgaggtgc agatcgacag actgatcacc ggaaggctgc agtccctgca gacctacgtt     4740

acccagcagc tgatcagagc cgccgagatt agagcctctg ccaatctggc cgccaccaag     4800

atgtctgagt gtgtgctggg ccagagcaag agagtggact tttgcggcaa gggctaccac     4860

ctgatgagct tccctcagtc tgcccctcac ggcgtggtgt ttctgcacgt gacatacgtg     4920

cccgctcaag agaagaattt caccaccgct ccagccatct gccacgacgg caaagcccac     4980

tttcctagag aaggcgtgtt cgtgtccaac ggcacccatt ggttcgtgac ccagcggaac     5040

ttctacgagc cccagatcat caccaccgac aacaccttcg tgtctggcaa ctgcgacgtc     5100

gtgatcggca ttgtgaacaa taccgtgtac gaccctctgc agcccgagct ggacagcttc     5160

aaagaggaac tggataagta ctttaagaac cacacaagcc ccgacgtgga cctgggcgat     5220

atcagcggaa tcaatgccag cgtcgtgaac atccagaaag agatcgaccg gctgaacgag     5280

gtggccaaga atctgaacga gagcctgatc gacctgcaag aactggggaa gtacgagcag     5340

tacatcaagt ggcccagcgg ccgcttggtc ccacgtggct cacccggatc tggatacatc     5400

ccggaggccc ctagggacgg tcaagcttac gtgagaaagg acggcgaatg ggttctgctg     5460

tcgaccttct tgggacatca tcatcatcat cactaatgaa attcgagctc gcggccgcat     5520

cgatcttaag tcgcgactcg agctagcaga tctttttccc tctgccaaaa attatgggga     5580

catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc     5640

aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca     5700

tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc     5760

tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt     5820

ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt     5880

tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac tagccagatt     5940

tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat gaagatccct     6000

cgacctgcag cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt     6060

atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg     6120

cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg     6180

gaaacctgtc gtgccagcgg atccgcatct caattagtca gcaaccatag tcccgcccct     6240

aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg     6300

actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa     6360

gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt gtttattgca     6420

gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt     6480

tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc     6540

cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt     6600

ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag     6660

ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca     6720

tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt     6780

tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc     6840

gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct     6900

ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg     6960

tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca     7020

agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact     7080

atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta     7140

acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta     7200

actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct     7260

tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt     7320

tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga     7380

tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca     7440

tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat     7500

caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg     7560

cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt     7620

agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag     7680

acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc     7740

gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag     7800

ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca     7860

tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa     7920

ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga     7980

tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata     8040

attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca     8100

agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg     8160

ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg     8220

ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg     8280

cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag     8340

gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac     8400

tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca     8460

tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag     8520

tgccacctg                                                             8529


<210>  10
<211>  229
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SARS-CoV-2 Spike protein receptor binding domain and 
       hexahistidine tag

<400>  10

Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn 
1               5                   10                  15      


Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val 
            20                  25                  30          


Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser 
        35                  40                  45              


Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val 
    50                  55                  60                  


Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp 
65                  70                  75                  80  


Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln 
                85                  90                  95      


Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr 
            100                 105                 110         


Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly 
        115                 120                 125             


Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys 
    130                 135                 140                 


Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr 
145                 150                 155                 160 


Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser 
                165                 170                 175     


Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
            180                 185                 190         


Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly 
        195                 200                 205             


Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe His 
    210                 215                 220                 


His His His His His 
225                 


