                         SEQUENCE LISTING

<110>  Diagnostics for the Real World, Ltd
 
<120>  Coronavirus Detection

<130>  P/81972.WO01

<150>  GB 2004203.2
<151>  2020-03-23

<150>  GB 2005062.1
<151>  2020-04-06

<160>  48    

<170>  PatentIn version 3.5

<210>  1
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  1
ctgttggtca acaagacggc a                                                 21


<210>  2
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  2
gtcaacaaac tgttggtcaa                                                   20


<210>  3
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  3
gtcaacaaac tgttggtcaa ca                                                22


<210>  4
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  4
cattacaggt ggtgttgttc agtt                                              24


<210>  5
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  5
caatagtctg aacaactggt gt                                                22


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  6
ctggtgtaag ttccatctct                                                   20


<210>  7
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  7
aggtgacaat ttgtccaccg ac                                                22


<210>  8
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  8
ggcagtgagg acaatcagca actac                                             25


<210>  9
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  9
gaggacaatc agacaactac tattc                                             25


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  10
acccgtcctt gattggcttg                                                   20


<210>  11
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  11
caaacaattg ttgaggttca acctc                                             25


<210>  12
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  12
gaggttcaac ctcaattaga gatgg                                             25


<210>  13
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  13
ggaaggtgta gagtttctta gagac                                             25


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  14
tagttgatga cccgtgtcct                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  15
tggggtccat tatcagacat                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  16
caacacgaac gtcatgatac                                                   20


<210>  17
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  17
catagaacga acaacgcac                                                    19


<210>  18
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  18
ctggttctaa atcacccatt ca                                                22


<210>  19
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  19
gaacctaaat tgggtagtct tgtag                                             25


<210>  20
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  20
ggaacctaaa ttgggtagtc ttg                                               23


<210>  21
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7 promoter sequence

<400>  21
aattctaata cgactcacta tagggagaag g                                      31


<210>  22
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7 promoter sequence

<400>  22
aattctaata cgactcacta taggggaaaa g                                      31


<210>  23
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7 promoter sequence

<400>  23
taatacgact cactatag                                                     18


<210>  24
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7 promoter sequence

<400>  24
aattctaata cgactcacta tagggaaagg a                                      31


<210>  25
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7 promoter sequence

<400>  25
aattctaata cgactcacta tagggaaagg                                        30


<210>  26
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7 promoter sequence

<400>  26
aattctaata cgactcacta taggggaaag a                                      31


<210>  27
<211>  101
<212>  DNA
<213>  Human coronavirus

<400>  27
aggagtgttc tgaagtagag gcttcagatt tagaagaagg tgaatcagag tgcatttctg       60

agacttcaac tgaacaagtt gacgtttctc atgagacttc t                          101


<210>  28
<211>  112
<212>  DNA
<213>  Human coronavirus

<400>  28
atgatggtcc aagtgtggag acatctgatt cacaagttga agaagatgta gaaatgtcgg       60

attttgttga tcttgaatct gtgattcagg attatgaaaa tgtttgtttt ga              112


<210>  29
<211>  127
<212>  DNA
<213>  Human coronavirus

<400>  29
acacttgttt tggtgttagt aaacctaatg ccattgatgt tgaacattta gagcttaaag       60

aaactgtttt tgttgaacct aaggatggtg gtcaattttt tgtttctggt gattatcttt      120

ggtatgt                                                                127


<210>  30
<211>  126
<212>  DNA
<213>  Human coronavirus

<400>  30
acatgccaat tgcagaccct acacattttg acattgaaga agttgaactt ttagatgcag       60

agtttgtaga accaggctgt ggtggtattt tggcagtaat agatgagcac gtcttttata      120

agaagg                                                                 126


<210>  31
<211>  193
<212>  DNA
<213>  Human coronavirus

<400>  31
actggtgaca atgacgatga agatgttgtt actggtgaca atgacgatga agatgttgtt       60

actggtgaca atgacgatga agatgttgtt actggtgaca atgacgatga agagattgtt      120

actggtgaca atgatgacca aattgttgtt actggtgatg atgtagatga tattgaaagt      180

gtctatgatt ttg                                                         193


<210>  32
<211>  115
<212>  DNA
<213>  Human coronavirus

<400>  32
aagaagaaga ggaagactgg ctggatgata ctactgagca atcagagatt gagccagaac       60

cagaacctac acctgaagaa ccagttaatc agtttactgg ttatttaaaa cttac           115


<210>  33
<211>  115
<212>  DNA
<213>  Human coronavirus

<400>  33
aagaagaaga ggaagactgg ctggatgata ctactgagca atcagagatt gagccagaac       60

cagaacctac acctgaagaa ccagttaatc agtttactgg ttatttaaaa cttac           115


<210>  34
<211>  112
<212>  DNA
<213>  Bat SARS-like coronavirus

<400>  34
aagaagaaga ggactggctt ggtgatgcta ctgaattatc ggagcatgaa ccagaaccag       60

aactaacacc tgaagaacca gttaaccagt ttactggtta tttaaaactt ac              112


<210>  35
<211>  187
<212>  DNA
<213>  Human coronavirus

<400>  35
aagagcaaga agaagattgg ttagatgatg atagtcaaca aactgttggt caacaagacg       60

gcagtgagga caatcagaca actactattc aaacaattgt tgaggttcaa cctcaattag      120

agatggaact tacaccagtt gttcagacta ttgaagtgaa tagttttagt ggttatttaa      180

aacttac                                                                187


<210>  36
<211>  187
<212>  DNA
<213>  Human coronavirus

<400>  36
aagagcaaga agaagattgg ttagatgatg atagtcaaca aactgttggt caacaagacg       60

gcagtgagga caatcagaca actactattc aaacaattgt tgaggttcaa cctcaattag      120

agatggaact tacaccagtt gttcagacta ttgaagtgaa tagttttagt ggttatttaa      180

aacttac                                                                187


<210>  37
<211>  187
<212>  DNA
<213>  Human coronavirus

<400>  37
aagagcaaga agaagattgg ttagatgatg atagtcaaca aactgttggt caacaagacg       60

gcagtgagga caatcagaca actactattc aaacaattgt tgaggttcaa cctcaattag      120

agatggaact tacaccagtt gttcagacta ttgaagtgaa tagttttagt ggttatttaa      180

aacttac                                                                187


<210>  38
<211>  436
<212>  DNA
<213>  Human coronavirus

<400>  38
agaactggat catggtggtc attcaatcct gagactaatt gccttttgaa cgttccattt       60

ggtggtacaa ctgtcgtacg tccactcgta gaggactcta ccagtgtaac tgctgttgta      120

accaatggcc acctcaaaat ggctggcatg catttcggtg cttgtgacta cgacagactt      180

cctaatgaag tcaccgtggc caaacccaat gtgctgattg ctttaaaaat ggtgaagcgg      240

caaagctacg gaactaattc cggcgttgcc atttaccata gatataaggc aggtaattac      300

aggagtccgc ctattacggc ggatattgaa cttgcattgc ttcgagctta ggctctttag      360

taagagtatc ttaattgatt ttaacgaatc tcaatttcat tgttatggca tcccctgctg      420

cacctcgtgc tgtttc                                                      436


<210>  39
<211>  452
<212>  DNA
<213>  Human coronavirus

<400>  39
agaaccggca gtttttggag tttcaaccca gaaacaaaca acttgatgtg tatagatatg       60

aaaggaacaa tgtttgttag gccaataatt gaggactacc ataccctgac ggtcacaata      120

atacgcggtc atctttacat tcaaggtata aaactaggta ctggctattc tttggcagat      180

ttgccagctt atatgactgt tgctaaggtc acatacctgt gcacatataa gcgtggtttt      240

cttgacaaga taagcgatac tagtggtttt gctgtttatg ttaagtccaa agtcggtaat      300

taccgactgc catcaaccca aaagggttca ggcatggaca ccgcattgtt gagaaataat      360

atctaaattt taaggatgtc ttttactcct ggtaagcaat ccagtagtag agcgtcctct      420

ggaaatcgtt ctggtaatgg catccttaag tg                                    452


<210>  40
<211>  438
<212>  DNA
<213>  Human coronavirus

<400>  40
cgtgttaaaa ctttttgggc ttttaatcct gaaactaatg caatcatctc tctccaggtt       60

tacggacata attattactt accggtgatg gctgcaccta caggtgttac attaacactt      120

cttagtggtg tacttcttgt tgatggccat aagattgcta ctcgtgttca agtgggtcag      180

ttgcctaaat atgtaatagt tgctacgcct agtaccacaa ttgtttgtga ccgtgttggt      240

cgctctgtta atgaaacaag ccagactggt tgggcattct acgtccgtgc taaacatggt      300

gatttttctg gtgttgcctc tcaggagggt gttttgtcag aaagagagaa gttgcttcat      360

ttaatctaaa ctaaacaaaa tggctagtgt aaattgggcc gatgacagag ctgctaggaa      420

gaaatttcct cctccttc                                                    438


<210>  41
<211>  450
<212>  DNA
<213>  Human coronavirus

<400>  41
cgtgctcgaa ctttttgggc atggaatcct gaggttaatg caatcactgt cacaaccgtg       60

ttgggacaga catactatca acccattcaa caagctccaa caggcattac tgtgaccttg      120

ctgagcggcg tgctttacgt tgacggacat agattggctt caggtgttca ggttcataac      180

ctacctgaat acatgacagt tgccgtgccg agcactacta taatttatag tagagtcgga      240

aggtccgtaa attcacaaaa tagcacaggc tgggttttct acgtacgagt aaaacacggt      300

gatttttctg cagtgagctc tcccatgagc aacatgacag aaaacgaaag attgcttcat      360

tttttctaaa ctgaacgaaa agatggctac agtcaaatgg gctgatgcat ctgaaccaca      420

acgtggtcgt cagggtagaa taccttattc                                       450


<210>  42
<211>  443
<212>  DNA
<213>  Human coronavirus

<400>  42
agaactggca gttggtggag ttttaaccca gagactaata atcttatgtg tattgatatg       60

aaaggtaaga tgtatgttag gccagttatt gaggactatc atacattaac ggctactgtt      120

atccgtggtc atctttatat acagggtgtt aaacttggca ctggttacac gcttgccgat      180

ttgcctgttt atgttactgt agctaaggtg caagtcctct gtacttataa acgtgccttt      240

ttagataagt tagatgttaa tagtggtttt gctgtttttg ttaagtctaa agttggtaac      300

tatcgtttac cttctagtaa atctagtggt atggatactg ccttgttgag agcttaaatc      360

taaactatta ggatgtctta tactcccggt catcatgctg gaagtagaag ctcctctgga      420

aatcgttcag gaatcctcaa gaa                                              443


<210>  43
<211>  404
<212>  DNA
<213>  Human coronavirus

<400>  43
gcagttgcat acgcactgta gtacagcgct gtgcatctaa taaacctcat gtgcttgaag       60

atccttgtcc tactggttac caacctgaat ggaatataag gtacaacact aggggtaata      120

cttatagcac tgcttggctt tgtgctctag gaaaggtttt accttttcat agatggcaca      180

ctatggttca aacatgcaca cctaatgtta ctatcaactg tcaagatcca gctggtggtg      240

cgcttatagc taggtgttgg taccttcatg aaggtcacca aactgctgca tttagagacg      300

tatttgttgt tttaaataaa cgaacaaatt aaaatgtctg ataatggacc ccaatcaaac      360

caacgtagtg ccccccgcat tacatttggt ggacccacag attc                       404


<210>  44
<211>  322
<212>  DNA
<213>  Human coronavirus

<400>  44
gcagttgcat acgcactgta gtacagcgct gtgcatctaa tatagcactg cttggctttg       60

tgctctagga aaggttttac cttttcatag atggcacact atggttcaaa catgcacacc      120

taatgttact atcaactgtc aagatccagc tggtggtgcg cttatagcta ggtgttggta      180

ccttcatgaa ggtcaccaaa ctgctgcatt tagagacgta cttgttgttt taaataaacg      240

aacaaattaa aatgtctgat aatggacccc aatcaaacca acgtagtgcc ccccgcatta      300

catttggtgg acccacagat tc                                               322


<210>  45
<211>  415
<212>  DNA
<213>  Bat SARS-like coronavirus

<400>  45
gtgtattgca tgcataaaga atgcagtata caagaatgtt gtgaaaatca accattccaa       60

cctgaagacc catgtccaat acattattat tcggactggt ttgtaaaaat tggacctcgc      120

aagtctgctc gcctagtaca actttgtgct ggtgaatatg gacacagagt tccaatacat      180

tatgaaatgt ttggcaatta tactatttca tgtgaaccac ttgaaataaa ttgtcaaaac      240

ccaccagttg gaagtctcat tgtacgttgt tcatatgatg ttgactttat ggagtatcac      300

gacgttcgtg ttgttctaga tttcatctaa acgaacaaac taaaatgtct gataatggac      360

cccaaccaaa tcagcgtagt gccccccgca ttacatttgg tggacccaca gattc           415


<210>  46
<211>  409
<212>  DNA
<213>  Human coronavirus

<400>  46
gctgcatttc accaagaatg tagtttacag tcatgtactc aacatcaacc atatgtagtt       60

gatgacccgt gtcctattca cttctattct aaatggtata ttagagtagg agctagaaaa      120

tcagcacctt taattgaatt gtgcstggat gaggctggtt ctaaatcacc cattcagtac      180

atcgatatcg gtaattatac agtttcctgt tyacctttta caattaattg ccaggaacct      240

aaattgggta gtcttgtagt gcgttgttcg ttctatgaag actttttaga gtatcatgac      300

gttcgtgttg ttttagattt catctaaacg aacaaactaa aatgtctgat aatggacccc      360

aaaatcagcg aaatgcaccc cgcattacgt ttggtggacc ctcagattc                  409


<210>  47
<211>  409
<212>  DNA
<213>  Human coronavirus

<400>  47
gctgcatttc accaagaatg tagtttacag tcatgtactc aacatcaacc atatgtagtt       60

gatgacccgt gtcctattca cttctattct aaatggtata ttagagtagg agctagaaaa      120

tcagcacctt taattgaatt gtgcstggat gaggctggtt ctaaatcacc cattcagtac      180

atcgatatcg gtaattatac agtttcctgt tyacctttta caattaattg ccaggaacct      240

aaattgggta gtcttgtagt gcgttgttcg ttctatgaag actttttaga gtatcatgac      300

gttcgtgttg ttttagattt catctaaacg aacaaactaa aatgtctgat aatggacccc      360

aaaatcagcg aaatgcaccc cgcattacgt ttggtggacc ctcagattc                  409


<210>  48
<211>  409
<212>  DNA
<213>  Human coronavirus

<400>  48
gctgcatttc accaagaatg tagtttacag tcatgtactc aacatcaacc atatgtagtt       60

gatgacccgt gtcctattca cttctattct aaatggtata ttagagtagg agctagaaaa      120

tcagcacctt taattgaatt gtgcstggat gaggctggtt ctaaatcacc cattcagtac      180

atcgatatcg gtaattatac agtttcctgt tyacctttta caattaattg ccaggaacct      240

aaattgggta gtcttgtagt gcgttgttcg ttctatgaag actttttaga gtatcatgac      300

gttcgtgttg ttttagattt catctaaacg aacaaactaa aatgtctgat aatggacccc      360

aaaatcagcg aaatgcaccc cgcattacgt ttggtggacc ctcagattc                  409


