               SEQUENCE LISTING

<110> TECHNISCHE UNIVERSITÄT WIEN

<120> Polypeptides with peroxidase activity

<130> R 77682

<150> EP20167727.5
<151> 2020-04-02
<150> EP20165108.0
<151> 2020-03-24

<160> 8

<170> BiSSAP 1.3.6

<210> 1
<211> 306
<212> PRT
<213> Armoracia rusticana


<400> 1
Gln Leu Thr Pro Thr Phe Tyr Asp Asn Ser Cys Pro Asn Val Ser Asn 
1               5                   10                  15      
Ile Val Arg Asp Thr Ile Val Asn Glu Leu Arg Ser Asp Pro Arg Ile 
            20                  25                  30          
Ala Ala Ser Ile Leu Arg Leu His Phe His Asp Cys Phe Val Asn Gly 
        35                  40                  45              
Cys Asp Ala Ser Ile Leu Leu Asp Asn Thr Thr Ser Phe Arg Thr Glu 
    50                  55                  60                  
Lys Asp Ala Phe Gly Asn Ala Asn Ser Ala Arg Gly Phe Pro Val Ile 
65                  70                  75                  80  
Asp Arg Met Lys Ala Ala Val Glu Ser Ala Cys Pro Arg Thr Val Ser 
                85                  90                  95      
Cys Ala Asp Leu Leu Thr Ile Ala Ala Gln Gln Ser Val Thr Leu Ala 
            100                 105                 110         
Gly Gly Pro Ser Trp Arg Val Pro Leu Gly Arg Arg Asp Ser Leu Gln 
        115                 120                 125             
Ala Phe Leu Asp Leu Ala Asn Ala Asn Leu Pro Ala Pro Phe Phe Thr 
    130                 135                 140                 
Leu Pro Gln Leu Lys Asp Ser Phe Arg Asn Val Gly Leu Asn Arg Ser 
145                 150                 155                 160 
Ser Asp Leu Val Ala Leu Ser Gly Gly His Thr Phe Gly Lys Asn Gln 
                165                 170                 175     
Cys Arg Phe Ile Met Asp Arg Leu Tyr Asn Phe Ser Asn Thr Gly Leu 
            180                 185                 190         
Pro Asp Pro Thr Leu Asn Thr Thr Tyr Leu Gln Thr Leu Arg Gly Leu 
        195                 200                 205             
Cys Pro Leu Asn Gly Asn Leu Ser Ala Leu Val Asp Phe Asp Leu Arg 
    210                 215                 220                 
Thr Pro Thr Ile Phe Asp Asn Lys Tyr Tyr Val Asn Leu Glu Glu Gln 
225                 230                 235                 240 
Lys Gly Leu Ile Gln Ser Asp Gln Glu Leu Phe Ser Ser Pro Asn Ala 
                245                 250                 255     
Thr Asp Thr Ile Pro Leu Val Arg Ser Phe Ala Asn Ser Thr Gln Thr 
            260                 265                 270         
Phe Phe Asn Ala Phe Val Glu Ala Met Asp Arg Met Gly Asn Ile Thr 
        275                 280                 285             
Pro Leu Thr Gly Thr Gln Gly Gln Ile Arg Leu Asn Cys Arg Val Val 
    290                 295                 300                 
Asn Ser 
305     

<210> 2
<211> 307
<212> PRT
<213> Artificial Sequence


<220> 
<223> recombinant wt HRP

<400> 2
Met Gln Leu Thr Pro Thr Phe Tyr Asp Asn Ser Cys Pro Asn Val Ser 
1               5                   10                  15      
Asn Ile Val Arg Asp Thr Ile Val Asn Glu Leu Arg Ser Asp Pro Arg 
            20                  25                  30          
Ile Ala Ala Ser Ile Leu Arg Leu His Phe His Asp Cys Phe Val Asn 
        35                  40                  45              
Gly Cys Asp Ala Ser Ile Leu Leu Asp Asn Thr Thr Ser Phe Arg Thr 
    50                  55                  60                  
Glu Lys Asp Ala Phe Gly Asn Ala Asn Ser Ala Arg Gly Phe Pro Val 
65                  70                  75                  80  
Ile Asp Arg Met Lys Ala Ala Val Glu Ser Ala Cys Pro Arg Thr Val 
                85                  90                  95      
Ser Cys Ala Asp Leu Leu Thr Ile Ala Ala Gln Gln Ser Val Thr Leu 
            100                 105                 110         
Ala Gly Gly Pro Ser Trp Arg Val Pro Leu Gly Arg Arg Asp Ser Leu 
        115                 120                 125             
Gln Ala Phe Leu Asp Leu Ala Asn Ala Asn Leu Pro Ala Pro Phe Phe 
    130                 135                 140                 
Thr Leu Pro Gln Leu Lys Asp Ser Phe Arg Asn Val Gly Leu Asn Arg 
145                 150                 155                 160 
Ser Ser Asp Leu Val Ala Leu Ser Gly Gly His Thr Phe Gly Lys Asn 
                165                 170                 175     
Gln Cys Arg Phe Ile Met Asp Arg Leu Tyr Asn Phe Ser Asn Thr Gly 
            180                 185                 190         
Leu Pro Asp Pro Thr Leu Asn Thr Thr Tyr Leu Gln Thr Leu Arg Gly 
        195                 200                 205             
Leu Cys Pro Leu Asn Gly Asn Leu Ser Ala Leu Val Asp Phe Asp Leu 
    210                 215                 220                 
Arg Thr Pro Thr Ile Phe Asp Asn Lys Tyr Tyr Val Asn Leu Glu Glu 
225                 230                 235                 240 
Gln Lys Gly Leu Ile Gln Ser Asp Gln Glu Leu Phe Ser Ser Pro Asn 
                245                 250                 255     
Ala Thr Asp Thr Ile Pro Leu Val Arg Ser Phe Ala Asn Ser Thr Gln 
            260                 265                 270         
Thr Phe Phe Asn Ala Phe Val Glu Ala Met Asp Arg Met Gly Asn Ile 
        275                 280                 285             
Thr Pro Leu Thr Gly Thr Gln Gly Gln Ile Arg Leu Asn Cys Arg Val 
    290                 295                 300                 
Val Asn Ser 
305         

<210> 3
<211> 306
<212> PRT
<213> Artificial Sequence


<220> 
<223> HRP N13D/N57S/P146Q/N175S/N255D/N268D/N275K

<400> 3
Gln Leu Thr Pro Thr Phe Tyr Asp Asn Ser Cys Pro Asp Val Ser Asn 
1               5                   10                  15      
Ile Val Arg Asp Thr Ile Val Asn Glu Leu Arg Ser Asp Pro Arg Ile 
            20                  25                  30          
Ala Ala Ser Ile Leu Arg Leu His Phe His Asp Cys Phe Val Asn Gly 
        35                  40                  45              
Cys Asp Ala Ser Ile Leu Leu Asp Ser Thr Thr Ser Phe Arg Thr Glu 
    50                  55                  60                  
Lys Asp Ala Phe Gly Asn Ala Asn Ser Ala Arg Gly Phe Pro Val Ile 
65                  70                  75                  80  
Asp Arg Met Lys Ala Ala Val Glu Ser Ala Cys Pro Arg Thr Val Ser 
                85                  90                  95      
Cys Ala Asp Leu Leu Thr Ile Ala Ala Gln Gln Ser Val Thr Leu Ala 
            100                 105                 110         
Gly Gly Pro Ser Trp Arg Val Pro Leu Gly Arg Arg Asp Ser Leu Gln 
        115                 120                 125             
Ala Phe Leu Asp Leu Ala Asn Ala Asn Leu Pro Ala Pro Phe Phe Thr 
    130                 135                 140                 
Leu Gln Gln Leu Lys Asp Ser Phe Arg Asn Val Gly Leu Asn Arg Ser 
145                 150                 155                 160 
Ser Asp Leu Val Ala Leu Ser Gly Gly His Thr Phe Gly Lys Ser Gln 
                165                 170                 175     
Cys Arg Phe Ile Met Asp Arg Leu Tyr Asn Phe Ser Asn Thr Gly Leu 
            180                 185                 190         
Pro Asp Pro Thr Leu Asn Thr Thr Tyr Leu Gln Thr Leu Arg Gly Leu 
        195                 200                 205             
Cys Pro Leu Asn Gly Asn Leu Ser Ala Leu Val Asp Phe Asp Leu Arg 
    210                 215                 220                 
Thr Pro Thr Ile Phe Asp Asn Lys Tyr Tyr Val Asn Leu Glu Glu Gln 
225                 230                 235                 240 
Lys Gly Leu Ile Gln Ser Asp Gln Glu Leu Phe Ser Ser Pro Asp Ala 
                245                 250                 255     
Thr Asp Thr Ile Pro Leu Val Arg Ser Phe Ala Asp Ser Thr Gln Thr 
            260                 265                 270         
Phe Phe Lys Ala Phe Val Glu Ala Met Asp Arg Met Gly Asn Ile Thr 
        275                 280                 285             
Pro Leu Thr Gly Thr Gln Gly Gln Ile Arg Leu Asn Cys Arg Val Val 
    290                 295                 300                 
Asn Ser 
305     

<210> 4
<211> 307
<212> PRT
<213> Artificial Sequence


<220> 
<223> recombinant HRP N13D/N57S/P146Q/N175S/N255D/N268D/N275K

<400> 4
Met Gln Leu Thr Pro Thr Phe Tyr Asp Asn Ser Cys Pro Asp Val Ser 
1               5                   10                  15      
Asn Ile Val Arg Asp Thr Ile Val Asn Glu Leu Arg Ser Asp Pro Arg 
            20                  25                  30          
Ile Ala Ala Ser Ile Leu Arg Leu His Phe His Asp Cys Phe Val Asn 
        35                  40                  45              
Gly Cys Asp Ala Ser Ile Leu Leu Asp Ser Thr Thr Ser Phe Arg Thr 
    50                  55                  60                  
Glu Lys Asp Ala Phe Gly Asn Ala Asn Ser Ala Arg Gly Phe Pro Val 
65                  70                  75                  80  
Ile Asp Arg Met Lys Ala Ala Val Glu Ser Ala Cys Pro Arg Thr Val 
                85                  90                  95      
Ser Cys Ala Asp Leu Leu Thr Ile Ala Ala Gln Gln Ser Val Thr Leu 
            100                 105                 110         
Ala Gly Gly Pro Ser Trp Arg Val Pro Leu Gly Arg Arg Asp Ser Leu 
        115                 120                 125             
Gln Ala Phe Leu Asp Leu Ala Asn Ala Asn Leu Pro Ala Pro Phe Phe 
    130                 135                 140                 
Thr Leu Gln Gln Leu Lys Asp Ser Phe Arg Asn Val Gly Leu Asn Arg 
145                 150                 155                 160 
Ser Ser Asp Leu Val Ala Leu Ser Gly Gly His Thr Phe Gly Lys Ser 
                165                 170                 175     
Gln Cys Arg Phe Ile Met Asp Arg Leu Tyr Asn Phe Ser Asn Thr Gly 
            180                 185                 190         
Leu Pro Asp Pro Thr Leu Asn Thr Thr Tyr Leu Gln Thr Leu Arg Gly 
        195                 200                 205             
Leu Cys Pro Leu Asn Gly Asn Leu Ser Ala Leu Val Asp Phe Asp Leu 
    210                 215                 220                 
Arg Thr Pro Thr Ile Phe Asp Asn Lys Tyr Tyr Val Asn Leu Glu Glu 
225                 230                 235                 240 
Gln Lys Gly Leu Ile Gln Ser Asp Gln Glu Leu Phe Ser Ser Pro Asp 
                245                 250                 255     
Ala Thr Asp Thr Ile Pro Leu Val Arg Ser Phe Ala Asp Ser Thr Gln 
            260                 265                 270         
Thr Phe Phe Lys Ala Phe Val Glu Ala Met Asp Arg Met Gly Asn Ile 
        275                 280                 285             
Thr Pro Leu Thr Gly Thr Gln Gly Gln Ile Arg Leu Asn Cys Arg Val 
    290                 295                 300                 
Val Asn Ser 
305         

<210> 5
<211> 28
<212> DNA
<213> Artificial Sequence


<220> 
<223> DHU_P146deg3_fwd

<220> 
<221> misc_difference
<222> 11
<223> /note="a or g or c or t"

<220> 
<221> misc_difference
<222> 12
<223> /note="a or g or c or t"

<400> 5
tttcacgctg nnkcaactga aagatagc                                       28


<210> 6
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> DHU_P146deg3_rev

<400> 6
aacggagccg gcagattagc gttt                                           24


<210> 7
<211> 27
<212> DNA
<213> Artificial Sequence


<220> 
<223> DHU_N275deg3_fwd

<220> 
<221> misc_difference
<222> 11
<223> /note="a or g or c or t"

<220> 
<221> misc_difference
<222> 12
<223> /note="a or g or c or t"

<400> 7
gacgtttttc nnkgcattcg tcgaagc                                        27


<210> 8
<211> 23
<212> DNA
<213> Artificial Sequence


<220> 
<223> DHU_N275deg3_rev

<400> 8
tgggtcgaat cggcaaatga acg                                            23


