                         SEQUENCE LISTING

<110>  University of Tasmania
 
<120>  DNA ALTERING PROTEINS AND USES THEREFOR

<130>  35557390/GDR/SJD

<150>  2020900913
<151>  2020-03-25

<160>  153   

<170>  PatentIn version 3.5

<210>  1
<211>  1053
<212>  PRT
<213>  Staphylococcus aureus

<400>  1

Met Lys Arg Asn Tyr Ile Leu Gly Leu Ala Ile Gly Ile Thr Ser Val 
1               5                   10                  15      


Gly Tyr Gly Ile Ile Asp Tyr Glu Thr Arg Asp Val Ile Asp Ala Gly 
            20                  25                  30          


Val Arg Leu Phe Lys Glu Ala Asn Val Glu Asn Asn Glu Gly Arg Arg 
        35                  40                  45              


Ser Lys Arg Gly Ala Arg Arg Leu Lys Arg Arg Arg Arg His Arg Ile 
    50                  55                  60                  


Gln Arg Val Lys Lys Leu Leu Phe Asp Tyr Asn Leu Leu Thr Asp His 
65                  70                  75                  80  


Ser Glu Leu Ser Gly Ile Asn Pro Tyr Glu Ala Arg Val Lys Gly Leu 
                85                  90                  95      


Ser Gln Lys Leu Ser Glu Glu Glu Phe Ser Ala Ala Leu Leu His Leu 
            100                 105                 110         


Ala Lys Arg Arg Gly Val His Asn Val Asn Glu Val Glu Glu Asp Thr 
        115                 120                 125             


Gly Asn Glu Leu Ser Thr Lys Glu Gln Ile Ser Arg Asn Ser Lys Ala 
    130                 135                 140                 


Leu Glu Glu Lys Tyr Val Ala Glu Leu Gln Leu Glu Arg Leu Lys Lys 
145                 150                 155                 160 


Asp Gly Glu Val Arg Gly Ser Ile Asn Arg Phe Lys Thr Ser Asp Tyr 
                165                 170                 175     


Val Lys Glu Ala Lys Gln Leu Leu Lys Val Gln Lys Ala Tyr His Gln 
            180                 185                 190         


Leu Asp Gln Ser Phe Ile Asp Thr Tyr Ile Asp Leu Leu Glu Thr Arg 
        195                 200                 205             


Arg Thr Tyr Tyr Glu Gly Pro Gly Glu Gly Ser Pro Phe Gly Trp Lys 
    210                 215                 220                 


Asp Ile Lys Glu Trp Tyr Glu Met Leu Met Gly His Cys Thr Tyr Phe 
225                 230                 235                 240 


Pro Glu Glu Leu Arg Ser Val Lys Tyr Ala Tyr Asn Ala Asp Leu Tyr 
                245                 250                 255     


Asn Ala Leu Asn Asp Leu Asn Asn Leu Val Ile Thr Arg Asp Glu Asn 
            260                 265                 270         


Glu Lys Leu Glu Tyr Tyr Glu Lys Phe Gln Ile Ile Glu Asn Val Phe 
        275                 280                 285             


Lys Gln Lys Lys Lys Pro Thr Leu Lys Gln Ile Ala Lys Glu Ile Leu 
    290                 295                 300                 


Val Asn Glu Glu Asp Ile Lys Gly Tyr Arg Val Thr Ser Thr Gly Lys 
305                 310                 315                 320 


Pro Glu Phe Thr Asn Leu Lys Val Tyr His Asp Ile Lys Asp Ile Thr 
                325                 330                 335     


Ala Arg Lys Glu Ile Ile Glu Asn Ala Glu Leu Leu Asp Gln Ile Ala 
            340                 345                 350         


Lys Ile Leu Thr Ile Tyr Gln Ser Ser Glu Asp Ile Gln Glu Glu Leu 
        355                 360                 365             


Thr Asn Leu Asn Ser Glu Leu Thr Gln Glu Glu Ile Glu Gln Ile Ser 
    370                 375                 380                 


Asn Leu Lys Gly Tyr Thr Gly Thr His Asn Leu Ser Leu Lys Ala Ile 
385                 390                 395                 400 


Asn Leu Ile Leu Asp Glu Leu Trp His Thr Asn Asp Asn Gln Ile Ala 
                405                 410                 415     


Ile Phe Asn Arg Leu Lys Leu Val Pro Lys Lys Val Asp Leu Ser Gln 
            420                 425                 430         


Gln Lys Glu Ile Pro Thr Thr Leu Val Asp Asp Phe Ile Leu Ser Pro 
        435                 440                 445             


Val Val Lys Arg Ser Phe Ile Gln Ser Ile Lys Val Ile Asn Ala Ile 
    450                 455                 460                 


Ile Lys Lys Tyr Gly Leu Pro Asn Asp Ile Ile Ile Glu Leu Ala Arg 
465                 470                 475                 480 


Glu Lys Asn Ser Lys Asp Ala Gln Lys Met Ile Asn Glu Met Gln Lys 
                485                 490                 495     


Arg Asn Arg Gln Thr Asn Glu Arg Ile Glu Glu Ile Ile Arg Thr Thr 
            500                 505                 510         


Gly Lys Glu Asn Ala Lys Tyr Leu Ile Glu Lys Ile Lys Leu His Asp 
        515                 520                 525             


Met Gln Glu Gly Lys Cys Leu Tyr Ser Leu Glu Ala Ile Pro Leu Glu 
    530                 535                 540                 


Asp Leu Leu Asn Asn Pro Phe Asn Tyr Glu Val Asp His Ile Ile Pro 
545                 550                 555                 560 


Arg Ser Val Ser Phe Asp Asn Ser Phe Asn Asn Lys Val Leu Val Lys 
                565                 570                 575     


Gln Glu Glu Asn Ser Lys Lys Gly Asn Arg Thr Pro Phe Gln Tyr Leu 
            580                 585                 590         


Ser Ser Ser Asp Ser Lys Ile Ser Tyr Glu Thr Phe Lys Lys His Ile 
        595                 600                 605             


Leu Asn Leu Ala Lys Gly Lys Gly Arg Ile Ser Lys Thr Lys Lys Glu 
    610                 615                 620                 


Tyr Leu Leu Glu Glu Arg Asp Ile Asn Arg Phe Ser Val Gln Lys Asp 
625                 630                 635                 640 


Phe Ile Asn Arg Asn Leu Val Asp Thr Arg Tyr Ala Thr Arg Gly Leu 
                645                 650                 655     


Met Asn Leu Leu Arg Ser Tyr Phe Arg Val Asn Asn Leu Asp Val Lys 
            660                 665                 670         


Val Lys Ser Ile Asn Gly Gly Phe Thr Ser Phe Leu Arg Arg Lys Trp 
        675                 680                 685             


Lys Phe Lys Lys Glu Arg Asn Lys Gly Tyr Lys His His Ala Glu Asp 
    690                 695                 700                 


Ala Leu Ile Ile Ala Asn Ala Asp Phe Ile Phe Lys Glu Trp Lys Lys 
705                 710                 715                 720 


Leu Asp Lys Ala Lys Lys Val Met Glu Asn Gln Met Phe Glu Glu Lys 
                725                 730                 735     


Gln Ala Glu Ser Met Pro Glu Ile Glu Thr Glu Gln Glu Tyr Lys Glu 
            740                 745                 750         


Ile Phe Ile Thr Pro His Gln Ile Lys His Ile Lys Asp Phe Lys Asp 
        755                 760                 765             


Tyr Lys Tyr Ser His Arg Val Asp Lys Lys Pro Asn Arg Glu Leu Ile 
    770                 775                 780                 


Asn Asp Thr Leu Tyr Ser Thr Arg Lys Asp Asp Lys Gly Asn Thr Leu 
785                 790                 795                 800 


Ile Val Asn Asn Leu Asn Gly Leu Tyr Asp Lys Asp Asn Asp Lys Leu 
                805                 810                 815     


Lys Lys Leu Ile Asn Lys Ser Pro Glu Lys Leu Leu Met Tyr His His 
            820                 825                 830         


Asp Pro Gln Thr Tyr Gln Lys Leu Lys Leu Ile Met Glu Gln Tyr Gly 
        835                 840                 845             


Asp Glu Lys Asn Pro Leu Tyr Lys Tyr Tyr Glu Glu Thr Gly Asn Tyr 
    850                 855                 860                 


Leu Thr Lys Tyr Ser Lys Lys Asp Asn Gly Pro Val Ile Lys Lys Ile 
865                 870                 875                 880 


Lys Tyr Tyr Gly Asn Lys Leu Asn Ala His Leu Asp Ile Thr Asp Asp 
                885                 890                 895     


Tyr Pro Asn Ser Arg Asn Lys Val Val Lys Leu Ser Leu Lys Pro Tyr 
            900                 905                 910         


Arg Phe Asp Val Tyr Leu Asp Asn Gly Val Tyr Lys Phe Val Thr Val 
        915                 920                 925             


Lys Asn Leu Asp Val Ile Lys Lys Glu Asn Tyr Tyr Glu Val Asn Ser 
    930                 935                 940                 


Lys Cys Tyr Glu Glu Ala Lys Lys Leu Lys Lys Ile Ser Asn Gln Ala 
945                 950                 955                 960 


Glu Phe Ile Ala Ser Phe Tyr Asn Asn Asp Leu Ile Lys Ile Asn Gly 
                965                 970                 975     


Glu Leu Tyr Arg Val Ile Gly Val Asn Asn Asp Leu Leu Asn Arg Ile 
            980                 985                 990         


Glu Val Asn Met Ile Asp Ile Thr  Tyr Arg Glu Tyr Leu  Glu Asn Met 
        995                 1000                 1005             


Asn Asp  Lys Arg Pro Pro Arg  Ile Ile Lys Thr Ile  Ala Ser Lys 
    1010                 1015                 1020             


Thr Gln  Ser Ile Lys Lys Tyr  Ser Thr Asp Ile Leu  Gly Asn Leu 
    1025                 1030                 1035             


Tyr Glu  Val Lys Ser Lys Lys  His Pro Gln Ile Ile  Lys Lys Gly 
    1040                 1045                 1050             


<210>  2
<211>  166
<212>  PRT
<213>  Escherichia coli

<400>  2

Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu Thr 
1               5                   10                  15      


Leu Ala Lys Arg Ala Arg Asp Glu Arg Glu Val Pro Val Gly Ala Val 
            20                  25                  30          


Leu Val Leu Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Ala Ile 
        35                  40                  45              


Gly Leu His Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg Gln 
    50                  55                  60                  


Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Ile Asp Ala Thr Leu Tyr 
65                  70                  75                  80  


Gly Thr Phe Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His Ser 
                85                  90                  95      


Arg Ile Gly Arg Val Val Phe Gly Val Arg Asn Ala Lys Thr Gly Ala 
            100                 105                 110         


Ala Gly Ser Leu Met Asp Val Leu His Tyr Pro Gly Met Asn His Arg 
        115                 120                 125             


Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu Leu 
    130                 135                 140                 


Cys Tyr Phe Phe Arg Met Pro Arg Gln Val Phe Asn Ala Gln Lys Lys 
145                 150                 155                 160 


Ala Gln Ser Ser Thr Asp 
                165     


<210>  3
<211>  166
<212>  PRT
<213>  Escherichia coli

<400>  3

Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu Thr 
1               5                   10                  15      


Leu Ala Lys Arg Ala Arg Asp Glu Arg Glu Val Pro Val Gly Ala Val 
            20                  25                  30          


Leu Val Leu Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Ala Ile 
        35                  40                  45              


Gly Leu His Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg Gln 
    50                  55                  60                  


Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Ile Asp Ala Thr Leu Tyr 
65                  70                  75                  80  


Val Thr Phe Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His Ser 
                85                  90                  95      


Arg Ile Gly Arg Val Val Phe Gly Val Arg Asn Ala Lys Thr Gly Ala 
            100                 105                 110         


Ala Gly Ser Leu Met Asp Val Leu His Tyr Pro Gly Met Asn His Arg 
        115                 120                 125             


Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu Leu 
    130                 135                 140                 


Cys Tyr Phe Phe Arg Met Pro Arg Gln Val Phe Asn Ala Gln Lys Lys 
145                 150                 155                 160 


Ala Gln Ser Ser Thr Asp 
                165     


<210>  4
<211>  166
<212>  PRT
<213>  Escherichia coli

<400>  4

Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu Thr 
1               5                   10                  15      


Leu Ala Lys Arg Ala Trp Asp Glu Arg Glu Val Pro Val Gly Ala Val 
            20                  25                  30          


Leu Val His Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Pro Ile 
        35                  40                  45              


Gly Arg His Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg Gln 
    50                  55                  60                  


Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Ile Asp Ala Thr Leu Tyr 
65                  70                  75                  80  


Val Thr Leu Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His Ser 
                85                  90                  95      


Arg Ile Gly Arg Val Val Phe Gly Ala Arg Asp Ala Lys Thr Gly Ala 
            100                 105                 110         


Ala Gly Ser Leu Met Asp Val Leu His His Pro Gly Met Asn His Arg 
        115                 120                 125             


Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu Leu 
    130                 135                 140                 


Ser Asp Phe Phe Arg Met Arg Arg Gln Glu Ile Lys Ala Gln Lys Lys 
145                 150                 155                 160 


Ala Gln Ser Ser Thr Asp 
                165     


<210>  5
<211>  1472
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  microABE I744

<400>  5

Gly Cys Cys Ala Cys Cys Met Lys Arg Asn Tyr Ile Leu Gly Leu Ala 
1               5                   10                  15      


Ile Gly Ile Thr Ser Val Gly Tyr Gly Ile Ile Asp Tyr Glu Thr Arg 
            20                  25                  30          


Asp Val Ile Asp Ala Gly Val Arg Leu Phe Lys Glu Ala Asn Val Glu 
        35                  40                  45              


Asn Asn Glu Gly Arg Arg Ser Lys Arg Gly Ala Arg Arg Leu Lys Arg 
    50                  55                  60                  


Arg Arg Arg His Arg Ile Gln Arg Val Lys Lys Leu Leu Phe Asp Tyr 
65                  70                  75                  80  


Asn Leu Leu Thr Asp His Ser Glu Leu Ser Gly Ile Asn Pro Tyr Glu 
                85                  90                  95      


Ala Arg Val Lys Gly Leu Ser Gln Lys Leu Ser Glu Glu Glu Phe Ser 
            100                 105                 110         


Ala Ala Leu Leu His Leu Ala Lys Arg Arg Gly Val His Asn Val Asn 
        115                 120                 125             


Glu Val Glu Glu Asp Thr Gly Asn Glu Leu Ser Thr Lys Glu Gln Ile 
    130                 135                 140                 


Ser Arg Asn Ser Lys Ala Leu Glu Glu Lys Tyr Val Ala Glu Leu Gln 
145                 150                 155                 160 


Leu Glu Arg Leu Lys Lys Asp Gly Glu Val Arg Gly Ser Ile Asn Arg 
                165                 170                 175     


Phe Lys Thr Ser Asp Tyr Val Lys Glu Ala Lys Gln Leu Leu Lys Val 
            180                 185                 190         


Gln Lys Ala Tyr His Gln Leu Asp Gln Ser Phe Ile Asp Thr Tyr Ile 
        195                 200                 205             


Asp Leu Leu Glu Thr Arg Arg Thr Tyr Tyr Glu Gly Pro Gly Glu Gly 
    210                 215                 220                 


Ser Pro Phe Gly Trp Lys Asp Ile Lys Glu Trp Tyr Glu Met Leu Met 
225                 230                 235                 240 


Gly His Cys Thr Tyr Phe Pro Glu Glu Leu Arg Ser Val Lys Tyr Ala 
                245                 250                 255     


Tyr Asn Ala Asp Leu Tyr Asn Ala Leu Asn Asp Leu Asn Asn Leu Val 
            260                 265                 270         


Ile Thr Arg Asp Glu Asn Glu Lys Leu Glu Tyr Tyr Glu Lys Phe Gln 
        275                 280                 285             


Ile Ile Glu Asn Val Phe Lys Gln Lys Lys Lys Pro Thr Leu Lys Gln 
    290                 295                 300                 


Ile Ala Lys Glu Ile Leu Val Asn Glu Glu Asp Ile Lys Gly Tyr Arg 
305                 310                 315                 320 


Val Thr Ser Thr Gly Lys Pro Glu Phe Thr Asn Leu Lys Val Tyr His 
                325                 330                 335     


Asp Ile Lys Asp Ile Thr Ala Arg Lys Glu Ile Ile Glu Asn Ala Glu 
            340                 345                 350         


Leu Leu Asp Gln Ile Ala Lys Ile Leu Thr Ile Tyr Gln Ser Ser Glu 
        355                 360                 365             


Asp Ile Gln Glu Glu Leu Thr Asn Leu Asn Ser Glu Leu Thr Gln Glu 
    370                 375                 380                 


Glu Ile Glu Gln Ile Ser Asn Leu Lys Gly Tyr Thr Gly Thr His Asn 
385                 390                 395                 400 


Leu Ser Leu Lys Ala Ile Asn Leu Ile Leu Asp Glu Leu Trp His Thr 
                405                 410                 415     


Asn Asp Asn Gln Ile Ala Ile Phe Asn Arg Leu Lys Leu Val Pro Lys 
            420                 425                 430         


Lys Val Asp Leu Ser Gln Gln Lys Glu Ile Pro Thr Thr Leu Val Asp 
        435                 440                 445             


Asp Phe Ile Leu Ser Pro Val Val Lys Arg Ser Phe Ile Gln Ser Ile 
    450                 455                 460                 


Lys Val Ile Asn Ala Ile Ile Lys Lys Tyr Gly Leu Pro Asn Asp Ile 
465                 470                 475                 480 


Ile Ile Glu Leu Ala Arg Glu Lys Asn Ser Lys Asp Ala Gln Lys Met 
                485                 490                 495     


Ile Asn Glu Met Gln Lys Arg Asn Arg Gln Thr Asn Glu Arg Ile Glu 
            500                 505                 510         


Glu Ile Ile Arg Thr Thr Gly Lys Glu Asn Ala Lys Tyr Leu Ile Glu 
        515                 520                 525             


Lys Ile Lys Leu His Asp Met Gln Glu Gly Lys Cys Leu Tyr Ser Leu 
    530                 535                 540                 


Glu Ala Ile Pro Leu Glu Asp Leu Leu Asn Asn Pro Phe Asn Tyr Glu 
545                 550                 555                 560 


Val Asp His Ile Ile Pro Arg Ser Val Ser Phe Asp Asn Ser Phe Asn 
                565                 570                 575     


Asn Lys Val Leu Val Lys Gln Glu Glu Asn Ser Lys Lys Gly Asn Arg 
            580                 585                 590         


Thr Pro Phe Gln Tyr Leu Ser Ser Ser Asp Ser Lys Ile Ser Tyr Glu 
        595                 600                 605             


Thr Phe Lys Lys His Ile Leu Asn Leu Ala Lys Gly Lys Gly Arg Ile 
    610                 615                 620                 


Ser Lys Thr Lys Lys Glu Tyr Leu Leu Glu Glu Arg Asp Ile Asn Arg 
625                 630                 635                 640 


Phe Ser Val Gln Lys Asp Phe Ile Asn Arg Asn Leu Val Asp Thr Arg 
                645                 650                 655     


Tyr Ala Thr Arg Gly Leu Met Asn Leu Leu Arg Ser Tyr Phe Arg Val 
            660                 665                 670         


Asn Asn Leu Asp Val Lys Val Lys Ser Ile Asn Gly Gly Phe Thr Ser 
        675                 680                 685             


Phe Leu Arg Arg Lys Trp Lys Phe Lys Lys Glu Arg Asn Lys Gly Tyr 
    690                 695                 700                 


Lys His His Ala Glu Asp Ala Leu Ile Ile Ala Asn Ala Asp Phe Ile 
705                 710                 715                 720 


Phe Lys Glu Trp Lys Lys Leu Asp Lys Ala Lys Lys Val Met Glu Asn 
                725                 730                 735     


Gln Met Phe Glu Glu Lys Gln Ala Glu Ser Met Pro Glu Ile Pro Lys 
            740                 745                 750         


Lys Lys Arg Lys Val Gly Ser Ser Gly Ser Pro Lys Lys Lys Arg Lys 
        755                 760                 765             


Val Gly Ser Asp Ala Leu Asp Asp Phe Asp Leu Asp Met Leu Gly Ser 
    770                 775                 780                 


Asp Ala Leu Asp Asp Phe Gly Gly Gly Ser Ser Glu Val Glu Phe Ser 
785                 790                 795                 800 


His Glu Tyr Trp Met Arg His Ala Leu Thr Leu Ala Lys Arg Ala Arg 
                805                 810                 815     


Asp Glu Arg Glu Val Pro Val Gly Ala Val Leu Val Leu Asn Asn Arg 
            820                 825                 830         


Val Ile Gly Glu Gly Trp Asn Arg Ala Ile Gly Leu His Asp Pro Thr 
        835                 840                 845             


Ala His Ala Glu Ile Met Ala Leu Arg Gln Gly Gly Leu Val Met Gln 
    850                 855                 860                 


Asn Tyr Arg Leu Ile Asp Ala Thr Leu Tyr Gly Thr Phe Glu Pro Cys 
865                 870                 875                 880 


Val Met Cys Ala Gly Ala Met Ile His Ser Arg Ile Gly Arg Val Val 
                885                 890                 895     


Phe Gly Val Arg Asn Ala Lys Thr Gly Ala Ala Gly Ser Leu Met Asp 
            900                 905                 910         


Val Leu His Tyr Pro Gly Met Asn His Arg Val Glu Ile Thr Glu Gly 
        915                 920                 925             


Ile Leu Ala Asp Glu Cys Ala Ala Leu Leu Cys Tyr Phe Phe Arg Met 
    930                 935                 940                 


Pro Arg Gln Val Phe Asn Ala Gln Lys Lys Ala Gln Ser Ser Thr Asp 
945                 950                 955                 960 


Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
                965                 970                 975     


Gly Ser Gly Gly Ile Glu Thr Glu Gln Glu Tyr Lys Glu Ile Phe Ile 
            980                 985                 990         


Thr Pro His Gln Ile Lys His Ile  Lys Asp Phe Lys Asp  Tyr Lys Tyr 
        995                 1000                 1005             


Ser His  Arg Val Asp Lys Lys  Pro Asn Arg Glu Leu  Ile Asn Asp 
    1010                 1015                 1020             


Thr Leu  Tyr Ser Thr Arg Lys  Asp Asp Lys Gly Asn  Thr Leu Ile 
    1025                 1030                 1035             


Val Asn  Asn Leu Asn Gly Leu  Tyr Asp Lys Asp Asn  Asp Lys Leu 
    1040                 1045                 1050             


Lys Lys  Leu Ile Asn Lys Ser  Pro Glu Lys Leu Leu  Met Tyr His 
    1055                 1060                 1065             


His Asp  Pro Gln Thr Tyr Gln  Lys Leu Lys Leu Ile  Met Glu Gln 
    1070                 1075                 1080             


Tyr Gly  Asp Glu Lys Asn Pro  Leu Tyr Lys Tyr Tyr  Glu Glu Thr 
    1085                 1090                 1095             


Gly Asn  Tyr Leu Thr Lys Tyr  Ser Lys Lys Asp Asn  Gly Pro Val 
    1100                 1105                 1110             


Ile Lys  Lys Ile Lys Tyr Tyr  Gly Asn Lys Leu Asn  Ala His Leu 
    1115                 1120                 1125             


Asp Ile  Thr Asp Asp Tyr Pro  Asn Ser Arg Asn Lys  Val Val Lys 
    1130                 1135                 1140             


Leu Ser  Leu Lys Pro Tyr Arg  Phe Asp Val Tyr Leu  Asp Asn Gly 
    1145                 1150                 1155             


Val Tyr  Lys Phe Val Thr Val  Lys Asn Leu Asp Val  Ile Lys Lys 
    1160                 1165                 1170             


Glu Asn  Tyr Tyr Glu Val Asn  Ser Lys Cys Tyr Glu  Glu Ala Lys 
    1175                 1180                 1185             


Lys Leu  Lys Lys Ile Ser Asn  Gln Ala Glu Phe Ile  Ala Ser Phe 
    1190                 1195                 1200             


Tyr Asn  Asn Asp Leu Ile Lys  Ile Asn Gly Glu Leu  Tyr Arg Val 
    1205                 1210                 1215             


Ile Gly  Val Asn Asn Asp Leu  Leu Asn Arg Ile Glu  Val Asn Met 
    1220                 1225                 1230             


Ile Asp  Ile Thr Tyr Arg Glu  Tyr Leu Glu Asn Met  Asn Asp Lys 
    1235                 1240                 1245             


Arg Pro  Pro Arg Ile Ile Lys  Thr Ile Ala Ser Lys  Thr Gln Ser 
    1250                 1255                 1260             


Ile Lys  Lys Tyr Ser Thr Asp  Ile Leu Gly Asn Leu  Tyr Glu Val 
    1265                 1270                 1275             


Lys Ser  Lys Lys His Pro Gln  Ile Ile Lys Lys Gly  Ser Gly Gly 
    1280                 1285                 1290             


Ser Lys  Arg Pro Ala Ala Thr  Lys Lys Ala Gly Gln  Ala Lys Lys 
    1295                 1300                 1305             


Lys Lys  Asp Tyr Lys Asp Asp  Asp Asp Lys Gly Ser  Gly Ala Thr 
    1310                 1315                 1320             


Asn Phe  Ser Leu Leu Lys Gln  Ala Gly Asp Val Glu  Glu Asn Pro 
    1325                 1330                 1335             


Gly Pro  Met Ala Lys Pro Leu  Ser Gln Glu Glu Ser  Thr Leu Ile 
    1340                 1345                 1350             


Glu Arg  Ala Thr Ala Thr Ile  Asn Ser Ile Pro Ile  Ser Glu Asp 
    1355                 1360                 1365             


Tyr Ser  Val Ala Ser Ala Ala  Leu Ser Ser Asp Gly  Arg Ile Phe 
    1370                 1375                 1380             


Thr Gly  Val Asn Val Tyr His  Phe Thr Gly Gly Pro  Cys Ala Glu 
    1385                 1390                 1395             


Leu Val  Val Leu Gly Thr Ala  Ala Ala Ala Ala Ala  Gly Asn Leu 
    1400                 1405                 1410             


Thr Cys  Ile Val Ala Ile Gly  Asn Glu Asn Arg Gly  Ile Leu Ser 
    1415                 1420                 1425             


Pro Cys  Gly Arg Cys Arg Gln  Val Leu Leu Asp Leu  His Pro Gly 
    1430                 1435                 1440             


Ile Lys  Ala Ile Val Lys Asp  Ser Asp Gly Gln Pro  Thr Ala Val 
    1445                 1450                 1455             


Gly Ile  Arg Glu Leu Leu Pro  Ser Gly Tyr Val Trp  Glu Gly 
    1460                 1465                 1470         


<210>  6
<211>  1667
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SaCas9-ABEmax

<400>  6

Gly Cys Cys Ala Cys Cys Met Lys Arg Thr Ala Asp Gly Ser Glu Phe 
1               5                   10                  15      


Glu Ser Pro Lys Lys Lys Arg Lys Val Ser Glu Val Glu Phe Ser His 
            20                  25                  30          


Glu Tyr Trp Met Arg His Ala Leu Thr Leu Ala Lys Arg Ala Trp Asp 
        35                  40                  45              


Glu Arg Glu Val Pro Val Gly Ala Val Leu Val His Asn Asn Arg Val 
    50                  55                  60                  


Ile Gly Glu Gly Trp Asn Arg Pro Ile Gly Arg His Asp Pro Thr Ala 
65                  70                  75                  80  


His Ala Glu Ile Met Ala Leu Arg Gln Gly Gly Leu Val Met Gln Asn 
                85                  90                  95      


Tyr Arg Leu Ile Asp Ala Thr Leu Tyr Val Thr Leu Glu Pro Cys Val 
            100                 105                 110         


Met Cys Ala Gly Ala Met Ile His Ser Arg Ile Gly Arg Val Val Phe 
        115                 120                 125             


Gly Ala Arg Asp Ala Lys Thr Gly Ala Ala Gly Ser Leu Met Asp Val 
    130                 135                 140                 


Leu His His Pro Gly Met Asn His Arg Val Glu Ile Thr Glu Gly Ile 
145                 150                 155                 160 


Leu Ala Asp Glu Cys Ala Ala Leu Leu Ser Asp Phe Phe Arg Met Arg 
                165                 170                 175     


Arg Gln Glu Ile Lys Ala Gln Lys Lys Ala Gln Ser Ser Thr Asp Ser 
            180                 185                 190         


Gly Gly Ser Ser Gly Gly Ser Ser Gly Ser Glu Thr Pro Gly Thr Ser 
        195                 200                 205             


Glu Ser Ala Thr Pro Glu Ser Ser Gly Gly Ser Ser Gly Gly Ser Ser 
    210                 215                 220                 


Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu Thr Leu 
225                 230                 235                 240 


Ala Lys Arg Ala Arg Asp Glu Arg Glu Val Pro Val Gly Ala Val Leu 
                245                 250                 255     


Val Leu Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Ala Ile Gly 
            260                 265                 270         


Leu His Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg Gln Gly 
        275                 280                 285             


Gly Leu Val Met Gln Asn Tyr Arg Leu Ile Asp Ala Thr Leu Tyr Val 
    290                 295                 300                 


Thr Phe Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His Ser Arg 
305                 310                 315                 320 


Ile Gly Arg Val Val Phe Gly Val Arg Asn Ala Lys Thr Gly Ala Ala 
                325                 330                 335     


Gly Ser Leu Met Asp Val Leu His Tyr Pro Gly Met Asn His Arg Val 
            340                 345                 350         


Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu Leu Cys 
        355                 360                 365             


Tyr Phe Phe Arg Met Pro Arg Gln Val Phe Asn Ala Gln Lys Lys Ala 
    370                 375                 380                 


Gln Ser Ser Thr Asp Ser Gly Gly Ser Ser Gly Gly Ser Ser Gly Ser 
385                 390                 395                 400 


Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Ser Gly Gly 
                405                 410                 415     


Ser Ser Gly Gly Ser Gly Lys Arg Asn Tyr Ile Leu Gly Leu Ala Ile 
            420                 425                 430         


Gly Ile Thr Ser Val Gly Tyr Gly Ile Ile Asp Tyr Glu Thr Arg Asp 
        435                 440                 445             


Val Ile Asp Ala Gly Val Arg Leu Phe Lys Glu Ala Asn Val Glu Asn 
    450                 455                 460                 


Asn Glu Gly Arg Arg Ser Lys Arg Gly Ala Arg Arg Leu Lys Arg Arg 
465                 470                 475                 480 


Arg Arg His Arg Ile Gln Arg Val Lys Lys Leu Leu Phe Asp Tyr Asn 
                485                 490                 495     


Leu Leu Thr Asp His Ser Glu Leu Ser Gly Ile Asn Pro Tyr Glu Ala 
            500                 505                 510         


Arg Val Lys Gly Leu Ser Gln Lys Leu Ser Glu Glu Glu Phe Ser Ala 
        515                 520                 525             


Ala Leu Leu His Leu Ala Lys Arg Arg Gly Val His Asn Val Asn Glu 
    530                 535                 540                 


Val Glu Glu Asp Thr Gly Asn Glu Leu Ser Thr Lys Glu Gln Ile Ser 
545                 550                 555                 560 


Arg Asn Ser Lys Ala Leu Glu Glu Lys Tyr Val Ala Glu Leu Gln Leu 
                565                 570                 575     


Glu Arg Leu Lys Lys Asp Gly Glu Val Arg Gly Ser Ile Asn Arg Phe 
            580                 585                 590         


Lys Thr Ser Asp Tyr Val Lys Glu Ala Lys Gln Leu Leu Lys Val Gln 
        595                 600                 605             


Lys Ala Tyr His Gln Leu Asp Gln Ser Phe Ile Asp Thr Tyr Ile Asp 
    610                 615                 620                 


Leu Leu Glu Thr Arg Arg Thr Tyr Tyr Glu Gly Pro Gly Glu Gly Ser 
625                 630                 635                 640 


Pro Phe Gly Trp Lys Asp Ile Lys Glu Trp Tyr Glu Met Leu Met Gly 
                645                 650                 655     


His Cys Thr Tyr Phe Pro Glu Glu Leu Arg Ser Val Lys Tyr Ala Tyr 
            660                 665                 670         


Asn Ala Asp Leu Tyr Asn Ala Leu Asn Asp Leu Asn Asn Leu Val Ile 
        675                 680                 685             


Thr Arg Asp Glu Asn Glu Lys Leu Glu Tyr Tyr Glu Lys Phe Gln Ile 
    690                 695                 700                 


Ile Glu Asn Val Phe Lys Gln Lys Lys Lys Pro Thr Leu Lys Gln Ile 
705                 710                 715                 720 


Ala Lys Glu Ile Leu Val Asn Glu Glu Asp Ile Lys Gly Tyr Arg Val 
                725                 730                 735     


Thr Ser Thr Gly Lys Pro Glu Phe Thr Asn Leu Lys Val Tyr His Asp 
            740                 745                 750         


Ile Lys Asp Ile Thr Ala Arg Lys Glu Ile Ile Glu Asn Ala Glu Leu 
        755                 760                 765             


Leu Asp Gln Ile Ala Lys Ile Leu Thr Ile Tyr Gln Ser Ser Glu Asp 
    770                 775                 780                 


Ile Gln Glu Glu Leu Thr Asn Leu Asn Ser Glu Leu Thr Gln Glu Glu 
785                 790                 795                 800 


Ile Glu Gln Ile Ser Asn Leu Lys Gly Tyr Thr Gly Thr His Asn Leu 
                805                 810                 815     


Ser Leu Lys Ala Ile Asn Leu Ile Leu Asp Glu Leu Trp His Thr Asn 
            820                 825                 830         


Asp Asn Gln Ile Ala Ile Phe Asn Arg Leu Lys Leu Val Pro Lys Lys 
        835                 840                 845             


Val Asp Leu Ser Gln Gln Lys Glu Ile Pro Thr Thr Leu Val Asp Asp 
    850                 855                 860                 


Phe Ile Leu Ser Pro Val Val Lys Arg Ser Phe Ile Gln Ser Ile Lys 
865                 870                 875                 880 


Val Ile Asn Ala Ile Ile Lys Lys Tyr Gly Leu Pro Asn Asp Ile Ile 
                885                 890                 895     


Ile Glu Leu Ala Arg Glu Lys Asn Ser Lys Asp Ala Gln Lys Met Ile 
            900                 905                 910         


Asn Glu Met Gln Lys Arg Asn Arg Gln Thr Asn Glu Arg Ile Glu Glu 
        915                 920                 925             


Ile Ile Arg Thr Thr Gly Lys Glu Asn Ala Lys Tyr Leu Ile Glu Lys 
    930                 935                 940                 


Ile Lys Leu His Asp Met Gln Glu Gly Lys Cys Leu Tyr Ser Leu Glu 
945                 950                 955                 960 


Ala Ile Pro Leu Glu Asp Leu Leu Asn Asn Pro Phe Asn Tyr Glu Val 
                965                 970                 975     


Asp His Ile Ile Pro Arg Ser Val Ser Phe Asp Asn Ser Phe Asn Asn 
            980                 985                 990         


Lys Val Leu Val Lys Gln Glu Glu  Asn Ser Lys Lys Gly  Asn Arg Thr 
        995                 1000                 1005             


Pro Phe  Gln Tyr Leu Ser Ser  Ser Asp Ser Lys Ile  Ser Tyr Glu 
    1010                 1015                 1020             


Thr Phe  Lys Lys His Ile Leu  Asn Leu Ala Lys Gly  Lys Gly Arg 
    1025                 1030                 1035             


Ile Ser  Lys Thr Lys Lys Glu  Tyr Leu Leu Glu Glu  Arg Asp Ile 
    1040                 1045                 1050             


Asn Arg  Phe Ser Val Gln Lys  Asp Phe Ile Asn Arg  Asn Leu Val 
    1055                 1060                 1065             


Asp Thr  Arg Tyr Ala Thr Arg  Gly Leu Met Asn Leu  Leu Arg Ser 
    1070                 1075                 1080             


Tyr Phe  Arg Val Asn Asn Leu  Asp Val Lys Val Lys  Ser Ile Asn 
    1085                 1090                 1095             


Gly Gly  Phe Thr Ser Phe Leu  Arg Arg Lys Trp Lys  Phe Lys Lys 
    1100                 1105                 1110             


Glu Arg  Asn Lys Gly Tyr Lys  His His Ala Glu Asp  Ala Leu Ile 
    1115                 1120                 1125             


Ile Ala  Asn Ala Asp Phe Ile  Phe Lys Glu Trp Lys  Lys Leu Asp 
    1130                 1135                 1140             


Lys Ala  Lys Lys Val Met Glu  Asn Gln Met Phe Glu  Glu Lys Gln 
    1145                 1150                 1155             


Ala Glu  Ser Met Pro Glu Ile  Glu Thr Glu Gln Glu  Tyr Lys Glu 
    1160                 1165                 1170             


Ile Phe  Ile Thr Pro His Gln  Ile Lys His Ile Lys  Asp Phe Lys 
    1175                 1180                 1185             


Asp Tyr  Lys Tyr Ser His Arg  Val Asp Lys Lys Pro  Asn Arg Glu 
    1190                 1195                 1200             


Leu Ile  Asn Asp Thr Leu Tyr  Ser Thr Arg Lys Asp  Asp Lys Gly 
    1205                 1210                 1215             


Asn Thr  Leu Ile Val Asn Asn  Leu Asn Gly Leu Tyr  Asp Lys Asp 
    1220                 1225                 1230             


Asn Asp  Lys Leu Lys Lys Leu  Ile Asn Lys Ser Pro  Glu Lys Leu 
    1235                 1240                 1245             


Leu Met  Tyr His His Asp Pro  Gln Thr Tyr Gln Lys  Leu Lys Leu 
    1250                 1255                 1260             


Ile Met  Glu Gln Tyr Gly Asp  Glu Lys Asn Pro Leu  Tyr Lys Tyr 
    1265                 1270                 1275             


Tyr Glu  Glu Thr Gly Asn Tyr  Leu Thr Lys Tyr Ser  Lys Lys Asp 
    1280                 1285                 1290             


Asn Gly  Pro Val Ile Lys Lys  Ile Lys Tyr Tyr Gly  Asn Lys Leu 
    1295                 1300                 1305             


Asn Ala  His Leu Asp Ile Thr  Asp Asp Tyr Pro Asn  Ser Arg Asn 
    1310                 1315                 1320             


Lys Val  Val Lys Leu Ser Leu  Lys Pro Tyr Arg Phe  Asp Val Tyr 
    1325                 1330                 1335             


Leu Asp  Asn Gly Val Tyr Lys  Phe Val Thr Val Lys  Asn Leu Asp 
    1340                 1345                 1350             


Val Ile  Lys Lys Glu Asn Tyr  Tyr Glu Val Asn Ser  Lys Cys Tyr 
    1355                 1360                 1365             


Glu Glu  Ala Lys Lys Leu Lys  Lys Ile Ser Asn Gln  Ala Glu Phe 
    1370                 1375                 1380             


Ile Ala  Ser Phe Tyr Asn Asn  Asp Leu Ile Lys Ile  Asn Gly Glu 
    1385                 1390                 1395             


Leu Tyr  Arg Val Ile Gly Val  Asn Asn Asp Leu Leu  Asn Arg Ile 
    1400                 1405                 1410             


Glu Val  Asn Met Ile Asp Ile  Thr Tyr Arg Glu Tyr  Leu Glu Asn 
    1415                 1420                 1425             


Met Asn  Asp Lys Arg Pro Pro  Arg Ile Ile Lys Thr  Ile Ala Ser 
    1430                 1435                 1440             


Lys Thr  Gln Ser Ile Lys Lys  Tyr Ser Thr Asp Ile  Leu Gly Asn 
    1445                 1450                 1455             


Leu Tyr  Glu Val Lys Ser Lys  Lys His Pro Gln Ile  Ile Lys Lys 
    1460                 1465                 1470             


Gly Ser  Gly Gly Ser Pro Lys  Lys Lys Arg Lys Val  Ser Gly Gly 
    1475                 1480                 1485             


Ser Lys  Arg Pro Ala Ala Thr  Lys Lys Ala Gly Gln  Ala Lys Lys 
    1490                 1495                 1500             


Lys Lys  Asp Tyr Lys Asp Asp  Asp Asp Lys Gly Ser  Gly Ala Thr 
    1505                 1510                 1515             


Asn Phe  Ser Leu Leu Lys Gln  Ala Gly Asp Val Glu  Glu Asn Pro 
    1520                 1525                 1530             


Gly Pro  Met Ala Lys Pro Leu  Ser Gln Glu Glu Ser  Thr Leu Ile 
    1535                 1540                 1545             


Glu Arg  Ala Thr Ala Thr Ile  Asn Ser Ile Pro Ile  Ser Glu Asp 
    1550                 1555                 1560             


Tyr Ser  Val Ala Ser Ala Ala  Leu Ser Ser Asp Gly  Arg Ile Phe 
    1565                 1570                 1575             


Thr Gly  Val Asn Val Tyr His  Phe Thr Gly Gly Pro  Cys Ala Glu 
    1580                 1585                 1590             


Leu Val  Val Leu Gly Thr Ala  Ala Ala Ala Ala Ala  Gly Asn Leu 
    1595                 1600                 1605             


Thr Cys  Ile Val Ala Ile Gly  Asn Glu Asn Arg Gly  Ile Leu Ser 
    1610                 1615                 1620             


Pro Cys  Gly Arg Cys Arg Gln  Val Leu Leu Asp Leu  His Pro Gly 
    1625                 1630                 1635             


Ile Lys  Ala Ile Val Lys Asp  Ser Asp Gly Gln Pro  Thr Ala Val 
    1640                 1645                 1650             


Gly Ile  Arg Glu Leu Leu Pro  Ser Gly Tyr Val Trp  Glu Gly 
    1655                 1660                 1665         


<210>  7
<211>  1469
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SaCas9-miniABEmax (V82G)

<400>  7

Gly Cys Cys Ala Cys Cys Met Lys Arg Thr Ala Asp Gly Ser Glu Phe 
1               5                   10                  15      


Glu Ser Pro Lys Lys Lys Arg Lys Val Ser Glu Val Glu Phe Ser His 
            20                  25                  30          


Glu Tyr Trp Met Arg His Ala Leu Thr Leu Ala Lys Arg Ala Arg Asp 
        35                  40                  45              


Glu Arg Glu Val Pro Val Gly Ala Val Leu Val Leu Asn Asn Arg Val 
    50                  55                  60                  


Ile Gly Glu Gly Trp Asn Arg Ala Ile Gly Leu His Asp Pro Thr Ala 
65                  70                  75                  80  


His Ala Glu Ile Met Ala Leu Arg Gln Gly Gly Leu Val Met Gln Asn 
                85                  90                  95      


Tyr Arg Leu Ile Asp Ala Thr Leu Tyr Gly Thr Phe Glu Pro Cys Val 
            100                 105                 110         


Met Cys Ala Gly Ala Met Ile His Ser Arg Ile Gly Arg Val Val Phe 
        115                 120                 125             


Gly Val Arg Asn Ala Lys Thr Gly Ala Ala Gly Ser Leu Met Asp Val 
    130                 135                 140                 


Leu His Tyr Pro Gly Met Asn His Arg Val Glu Ile Thr Glu Gly Ile 
145                 150                 155                 160 


Leu Ala Asp Glu Cys Ala Ala Leu Leu Cys Tyr Phe Phe Arg Met Pro 
                165                 170                 175     


Arg Gln Val Phe Asn Ala Gln Lys Lys Ala Gln Ser Ser Thr Asp Ser 
            180                 185                 190         


Gly Gly Ser Ser Gly Gly Ser Ser Gly Ser Glu Thr Pro Gly Thr Ser 
        195                 200                 205             


Glu Ser Ala Thr Pro Glu Ser Ser Gly Gly Ser Ser Gly Gly Ser Gly 
    210                 215                 220                 


Lys Arg Asn Tyr Ile Leu Gly Leu Ala Ile Gly Ile Thr Ser Val Gly 
225                 230                 235                 240 


Tyr Gly Ile Ile Asp Tyr Glu Thr Arg Asp Val Ile Asp Ala Gly Val 
                245                 250                 255     


Arg Leu Phe Lys Glu Ala Asn Val Glu Asn Asn Glu Gly Arg Arg Ser 
            260                 265                 270         


Lys Arg Gly Ala Arg Arg Leu Lys Arg Arg Arg Arg His Arg Ile Gln 
        275                 280                 285             


Arg Val Lys Lys Leu Leu Phe Asp Tyr Asn Leu Leu Thr Asp His Ser 
    290                 295                 300                 


Glu Leu Ser Gly Ile Asn Pro Tyr Glu Ala Arg Val Lys Gly Leu Ser 
305                 310                 315                 320 


Gln Lys Leu Ser Glu Glu Glu Phe Ser Ala Ala Leu Leu His Leu Ala 
                325                 330                 335     


Lys Arg Arg Gly Val His Asn Val Asn Glu Val Glu Glu Asp Thr Gly 
            340                 345                 350         


Asn Glu Leu Ser Thr Lys Glu Gln Ile Ser Arg Asn Ser Lys Ala Leu 
        355                 360                 365             


Glu Glu Lys Tyr Val Ala Glu Leu Gln Leu Glu Arg Leu Lys Lys Asp 
    370                 375                 380                 


Gly Glu Val Arg Gly Ser Ile Asn Arg Phe Lys Thr Ser Asp Tyr Val 
385                 390                 395                 400 


Lys Glu Ala Lys Gln Leu Leu Lys Val Gln Lys Ala Tyr His Gln Leu 
                405                 410                 415     


Asp Gln Ser Phe Ile Asp Thr Tyr Ile Asp Leu Leu Glu Thr Arg Arg 
            420                 425                 430         


Thr Tyr Tyr Glu Gly Pro Gly Glu Gly Ser Pro Phe Gly Trp Lys Asp 
        435                 440                 445             


Ile Lys Glu Trp Tyr Glu Met Leu Met Gly His Cys Thr Tyr Phe Pro 
    450                 455                 460                 


Glu Glu Leu Arg Ser Val Lys Tyr Ala Tyr Asn Ala Asp Leu Tyr Asn 
465                 470                 475                 480 


Ala Leu Asn Asp Leu Asn Asn Leu Val Ile Thr Arg Asp Glu Asn Glu 
                485                 490                 495     


Lys Leu Glu Tyr Tyr Glu Lys Phe Gln Ile Ile Glu Asn Val Phe Lys 
            500                 505                 510         


Gln Lys Lys Lys Pro Thr Leu Lys Gln Ile Ala Lys Glu Ile Leu Val 
        515                 520                 525             


Asn Glu Glu Asp Ile Lys Gly Tyr Arg Val Thr Ser Thr Gly Lys Pro 
    530                 535                 540                 


Glu Phe Thr Asn Leu Lys Val Tyr His Asp Ile Lys Asp Ile Thr Ala 
545                 550                 555                 560 


Arg Lys Glu Ile Ile Glu Asn Ala Glu Leu Leu Asp Gln Ile Ala Lys 
                565                 570                 575     


Ile Leu Thr Ile Tyr Gln Ser Ser Glu Asp Ile Gln Glu Glu Leu Thr 
            580                 585                 590         


Asn Leu Asn Ser Glu Leu Thr Gln Glu Glu Ile Glu Gln Ile Ser Asn 
        595                 600                 605             


Leu Lys Gly Tyr Thr Gly Thr His Asn Leu Ser Leu Lys Ala Ile Asn 
    610                 615                 620                 


Leu Ile Leu Asp Glu Leu Trp His Thr Asn Asp Asn Gln Ile Ala Ile 
625                 630                 635                 640 


Phe Asn Arg Leu Lys Leu Val Pro Lys Lys Val Asp Leu Ser Gln Gln 
                645                 650                 655     


Lys Glu Ile Pro Thr Thr Leu Val Asp Asp Phe Ile Leu Ser Pro Val 
            660                 665                 670         


Val Lys Arg Ser Phe Ile Gln Ser Ile Lys Val Ile Asn Ala Ile Ile 
        675                 680                 685             


Lys Lys Tyr Gly Leu Pro Asn Asp Ile Ile Ile Glu Leu Ala Arg Glu 
    690                 695                 700                 


Lys Asn Ser Lys Asp Ala Gln Lys Met Ile Asn Glu Met Gln Lys Arg 
705                 710                 715                 720 


Asn Arg Gln Thr Asn Glu Arg Ile Glu Glu Ile Ile Arg Thr Thr Gly 
                725                 730                 735     


Lys Glu Asn Ala Lys Tyr Leu Ile Glu Lys Ile Lys Leu His Asp Met 
            740                 745                 750         


Gln Glu Gly Lys Cys Leu Tyr Ser Leu Glu Ala Ile Pro Leu Glu Asp 
        755                 760                 765             


Leu Leu Asn Asn Pro Phe Asn Tyr Glu Val Asp His Ile Ile Pro Arg 
    770                 775                 780                 


Ser Val Ser Phe Asp Asn Ser Phe Asn Asn Lys Val Leu Val Lys Gln 
785                 790                 795                 800 


Glu Glu Asn Ser Lys Lys Gly Asn Arg Thr Pro Phe Gln Tyr Leu Ser 
                805                 810                 815     


Ser Ser Asp Ser Lys Ile Ser Tyr Glu Thr Phe Lys Lys His Ile Leu 
            820                 825                 830         


Asn Leu Ala Lys Gly Lys Gly Arg Ile Ser Lys Thr Lys Lys Glu Tyr 
        835                 840                 845             


Leu Leu Glu Glu Arg Asp Ile Asn Arg Phe Ser Val Gln Lys Asp Phe 
    850                 855                 860                 


Ile Asn Arg Asn Leu Val Asp Thr Arg Tyr Ala Thr Arg Gly Leu Met 
865                 870                 875                 880 


Asn Leu Leu Arg Ser Tyr Phe Arg Val Asn Asn Leu Asp Val Lys Val 
                885                 890                 895     


Lys Ser Ile Asn Gly Gly Phe Thr Ser Phe Leu Arg Arg Lys Trp Lys 
            900                 905                 910         


Phe Lys Lys Glu Arg Asn Lys Gly Tyr Lys His His Ala Glu Asp Ala 
        915                 920                 925             


Leu Ile Ile Ala Asn Ala Asp Phe Ile Phe Lys Glu Trp Lys Lys Leu 
    930                 935                 940                 


Asp Lys Ala Lys Lys Val Met Glu Asn Gln Met Phe Glu Glu Lys Gln 
945                 950                 955                 960 


Ala Glu Ser Met Pro Glu Ile Glu Thr Glu Gln Glu Tyr Lys Glu Ile 
                965                 970                 975     


Phe Ile Thr Pro His Gln Ile Lys His Ile Lys Asp Phe Lys Asp Tyr 
            980                 985                 990         


Lys Tyr Ser His Arg Val Asp Lys  Lys Pro Asn Arg Glu  Leu Ile Asn 
        995                 1000                 1005             


Asp Thr  Leu Tyr Ser Thr Arg  Lys Asp Asp Lys Gly  Asn Thr Leu 
    1010                 1015                 1020             


Ile Val  Asn Asn Leu Asn Gly  Leu Tyr Asp Lys Asp  Asn Asp Lys 
    1025                 1030                 1035             


Leu Lys  Lys Leu Ile Asn Lys  Ser Pro Glu Lys Leu  Leu Met Tyr 
    1040                 1045                 1050             


His His  Asp Pro Gln Thr Tyr  Gln Lys Leu Lys Leu  Ile Met Glu 
    1055                 1060                 1065             


Gln Tyr  Gly Asp Glu Lys Asn  Pro Leu Tyr Lys Tyr  Tyr Glu Glu 
    1070                 1075                 1080             


Thr Gly  Asn Tyr Leu Thr Lys  Tyr Ser Lys Lys Asp  Asn Gly Pro 
    1085                 1090                 1095             


Val Ile  Lys Lys Ile Lys Tyr  Tyr Gly Asn Lys Leu  Asn Ala His 
    1100                 1105                 1110             


Leu Asp  Ile Thr Asp Asp Tyr  Pro Asn Ser Arg Asn  Lys Val Val 
    1115                 1120                 1125             


Lys Leu  Ser Leu Lys Pro Tyr  Arg Phe Asp Val Tyr  Leu Asp Asn 
    1130                 1135                 1140             


Gly Val  Tyr Lys Phe Val Thr  Val Lys Asn Leu Asp  Val Ile Lys 
    1145                 1150                 1155             


Lys Glu  Asn Tyr Tyr Glu Val  Asn Ser Lys Cys Tyr  Glu Glu Ala 
    1160                 1165                 1170             


Lys Lys  Leu Lys Lys Ile Ser  Asn Gln Ala Glu Phe  Ile Ala Ser 
    1175                 1180                 1185             


Phe Tyr  Asn Asn Asp Leu Ile  Lys Ile Asn Gly Glu  Leu Tyr Arg 
    1190                 1195                 1200             


Val Ile  Gly Val Asn Asn Asp  Leu Leu Asn Arg Ile  Glu Val Asn 
    1205                 1210                 1215             


Met Ile  Asp Ile Thr Tyr Arg  Glu Tyr Leu Glu Asn  Met Asn Asp 
    1220                 1225                 1230             


Lys Arg  Pro Pro Arg Ile Ile  Lys Thr Ile Ala Ser  Lys Thr Gln 
    1235                 1240                 1245             


Ser Ile  Lys Lys Tyr Ser Thr  Asp Ile Leu Gly Asn  Leu Tyr Glu 
    1250                 1255                 1260             


Val Lys  Ser Lys Lys His Pro  Gln Ile Ile Lys Lys  Gly Ser Gly 
    1265                 1270                 1275             


Gly Ser  Pro Lys Lys Lys Arg  Lys Val Ser Gly Gly  Ser Lys Arg 
    1280                 1285                 1290             


Pro Ala  Ala Thr Lys Lys Ala  Gly Gln Ala Lys Lys  Lys Lys Asp 
    1295                 1300                 1305             


Tyr Lys  Asp Asp Asp Asp Lys  Gly Ser Gly Ala Thr  Asn Phe Ser 
    1310                 1315                 1320             


Leu Leu  Lys Gln Ala Gly Asp  Val Glu Glu Asn Pro  Gly Pro Met 
    1325                 1330                 1335             


Ala Lys  Pro Leu Ser Gln Glu  Glu Ser Thr Leu Ile  Glu Arg Ala 
    1340                 1345                 1350             


Thr Ala  Thr Ile Asn Ser Ile  Pro Ile Ser Glu Asp  Tyr Ser Val 
    1355                 1360                 1365             


Ala Ser  Ala Ala Leu Ser Ser  Asp Gly Arg Ile Phe  Thr Gly Val 
    1370                 1375                 1380             


Asn Val  Tyr His Phe Thr Gly  Gly Pro Cys Ala Glu  Leu Val Val 
    1385                 1390                 1395             


Leu Gly  Thr Ala Ala Ala Ala  Ala Ala Gly Asn Leu  Thr Cys Ile 
    1400                 1405                 1410             


Val Ala  Ile Gly Asn Glu Asn  Arg Gly Ile Leu Ser  Pro Cys Gly 
    1415                 1420                 1425             


Arg Cys  Arg Gln Val Leu Leu  Asp Leu His Pro Gly  Ile Lys Ala 
    1430                 1435                 1440             


Ile Val  Lys Asp Ser Asp Gly  Gln Pro Thr Ala Val  Gly Ile Arg 
    1445                 1450                 1455             


Glu Leu  Leu Pro Ser Gly Tyr  Val Trp Glu Gly 
    1460                 1465                 


<210>  8
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CBE-YFP FWD

<400>  8
tcgtcggcag cgtcagatgt gtataagaga caggcgccaa gatccattcg tt               52


<210>  9
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CBE-YFP REV

<400>  9
gtctcgtggg ctcggagatg tgtataagag acagcttgcc ggtggtgcag at               52


<210>  10
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ABE-site16 FWD

<400>  10
tcgtcggcag cgtcagatgt gtataagaga cagttctcta tccacctgga atgagttt         58


<210>  11
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ABE-site16 REV

<400>  11
gtctcgtggg ctcggagatg tgtataagag acagcagcaa tccagcaaca cgc              53


<210>  12
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ABE-YFP FWD

<400>  12
tcgtcggcag cgtcagatgt gtataagaga cagcttcttc aagtccgcca tgc              53


<210>  13
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ABE-YFP REV

<400>  13
gtctcgtggg ctcggagatg tgtataagag acagccttga tgccgttctt ctgc             54


<210>  14
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RNCF2 site1 FWD

<400>  14
tcgtcggcag cgtcagatgt gtataagaga cagtgtgcag acaaacggaa ctcaacc          57


<210>  15
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RNCF2 site1 REV

<400>  15
gtctcgtggg ctcggagatg tgtataagag acagccaaca tacagaagtc aggaatgctt       60

ga                                                                      62


<210>  16
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNAJB1 (RNA site1) FWD

<400>  16
tcgtcggcag cgtcagatgt gtataagaga cagcatggca tgagggtctc catgg            55


<210>  17
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNAJB1 (RNA site1) REV

<400>  17
gtctcgtggg ctcggagatg tgtataagag acaggcgcta ccacccggac aag              53


<210>  18
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MTA2 (RNA site2) FWD

<400>  18
tcgtcggcag cgtcagatgt gtataagaga caggatgtga gggtgcaggt agaggg           56


<210>  19
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MTA2 (RNA site2) REV

<400>  19
gtctcgtggg ctcggagatg tgtataagag acaggggatt cgttcaagct cacagcc          57


<210>  20
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PTBP2 (RNA site3) FWD

<400>  20
tcgtcggcag cgtcagatgt gtataagaga cagagatttt ggtaattccc cattgcatcg       60


<210>  21
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PTBP2 (RNA site3) REV

<400>  21
gtctcgtggg ctcggagatg tgtataagag acagcctgaa tagcttcttc cactgttgcc       60


<210>  22
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SAP30BP (RNA site4) FWD

<400>  22
tcgtcggcag cgtcagatgt gtataagaga cagcagaacc ccctggcaga tgttc            55


<210>  23
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SAP30BP (RNA site4) REV

<400>  23
gtctcgtggg ctcggagatg tgtataagag acagtcctca gaccagccat gggg             54


<210>  24
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LCMT1 (RNA site5) FWD

<400>  24
tcgtcggcag cgtcagatgt gtataagaga cagattgcca acactcctga tagctgaatg       60


<210>  25
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LCMT1 (RNA site5) REV

<400>  25
gtctcgtggg ctcggagatg tgtataagag acaggacttg caggtctcca ctcccg           56


<210>  26
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SCAP (RNA site6) FWD

<400>  26
tcgtcggcag cgtcagatgt gtataagaga caggaagtag acaacacgca gcctcttg         58


<210>  27
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SCAP (RNA site6) REV

<400>  27
gtctcgtggg ctcggagatg tgtataagag acagccattg acattcgccg gatggag          57


<210>  28
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  YFP (CBE-AIDx) FWD

<400>  28
tcgtcggcag cgtcagatgt gtataagaga cagcgcaaat gggcggtagg cgtg             54


<210>  29
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  YFP (CBE-AIDx) REV

<400>  29
gtctcgtggg ctcggagatg tgtataagag acaggaagtc gtgctgcttc atgtgg           56


<210>  30
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SpCas9 sgRNA YFP ABE top

<400>  30
accggtgaac cgcatcgagc tgaa                                              24


<210>  31
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SpCas9 sgRNA YFP ABE btm

<400>  31
aacttcagct cgatgcggtt cacc                                              24


<210>  32
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SpCas9 ABE16 sgRNA top

<400>  32
accggggaat aaatcataga atcc                                              24


<210>  33
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SpCas9 ABE16 sgRNA btm

<400>  33
aacggattct atgatttatt cccc                                              24


<210>  34
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SpCas9 RNF2 sgRNA top

<400>  34
accggtcatc ttagtcatta cctg                                              24


<210>  35
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SpCas9 RNF2 sgRNA btm

<400>  35
aaccaggtaa tgactaagat gacc                                              24


<210>  36
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SaCas9 YFP sgRNA (SapI) top

<400>  36
accgcgcgcc aagatccatt cgttg                                             25


<210>  37
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SaCas9 YFP sgRNA (SapI) bottom

<400>  37
aaccaacgaa tggatcttgg cgcgc                                             25


<210>  38
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SaCas9 ABE16 sgRNA top (SapI)

<400>  38
accgggacaa ggtttgaagg acagg                                             25


<210>  39
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SaCas9 ABE ABE 16 sgRNA btm (SapI)

<400>  39
aaccctgtcc ttcaaacctt gtccc                                             25


<210>  40
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SaCas9 RNCF2 sgRNA top (SapI)

<400>  40
accgcatgaa aacttaaata gaaca                                             25


<210>  41
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SaCas9 RNCF2 sgRNA btm (SapI)

<400>  41
aactgttcta tttaagtttt catgc                                             25


<210>  42
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SaCas9 PCDH15 sgRNA top

<400>  42
accgggttca cctctcattc agatt                                             25


<210>  43
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SaCas9 PCDH15 sgRNA bottom

<400>  43
aacaatctga atgagaggtg aaccc                                             25


<210>  44
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SpCas9 ABE16 target guide (ABE)

<400>  44
gggaataaat catagaatcc                                                   20


<210>  45
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SaCas9 ABE16 target guide (ABE)

<400>  45
ggacaaggtt tgaaggacag g                                                 21


<210>  46
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SpCas9 YFP target guide (ABE)

<400>  46
gtgaaccgca tcgagctgaa                                                   20


<210>  47
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SaCas9 RNF2 target guide (ABE)

<400>  47
catgaaaact taaatagaac a                                                 21


<210>  48
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SpCas9 YFP target guide (CBE)

<400>  48
ccgtccagct cgaccaggat                                                   20


<210>  49
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SpCas9 RNF2 target guide (CBE)

<400>  49
gtcatcttag tcattacctg                                                   20


<210>  50
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SaCas9 PCDH15 target guide (ABE)

<400>  50
ggttcacctc tcattcagat t                                                 21


<210>  51
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SaCas9 ABE1 target guide (ABE)

<400>  51
ctgaatagct gcaaacaagt g                                                 21


<210>  52
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SaCas9 ABE3 target guide (ABE)

<400>  52
cggtgaactc agagatagaa a                                                 21


<210>  53
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SaCas9 ABE4 target guide (ABE)

<400>  53
tgaagggaaa tctggagcaa a                                                 21


<210>  54
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SaCas9 ABE5 target guide (ABE/CBE)

<400>  54
ctcagaaagc tcaaagtgtg a                                                 21


<210>  55
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SaCas9 ABE7 target guide (ABE)

<400>  55
aagaatacta agcatagact c                                                 21


<210>  56
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SaCas9 ABE8 target guide (ABE)

<400>  56
tgggctttag gaacaggggt a                                                 21


<210>  57
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SaCas9 ABE9 target guide (ABE/CBE)

<400>  57
aacaacaaaa cggacagtga a                                                 21


<210>  58
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SaCas9 ABE11 target guide (ABE)

<400>  58
gggagctaga caaaatggag t                                                 21


<210>  59
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SaCas9 HEKsite4 target guide (ABE/CBE)

<400>  59
ggcgaggcag agggtccaaa g                                                 21


<210>  60
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SaCas9 ABE17 target guide (ABE)

<400>  60
atttacagcc tggcctttgg gg                                                22


<210>  61
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SaCas9 ABE18 target guide (ABE)

<400>  61
ggagagaaag agaagttgat tg                                                22


<210>  62
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SaCas9 ABE19 target guide (ABE)

<400>  62
gtgtcaggta atgtgctaaa ca                                                22


<210>  63
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SaCas9 ABE21 target guide (ABE)

<400>  63
gctgttgcat gaggaaaggg ac                                                22


<210>  64
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SaCas9 ABE27 target guide (ABE)

<400>  64
gggagctcaa gcctgattcc aa                                                22


<210>  65
<211>  200
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ABE16

<400>  65
cagcaatcca gcaacacgcg gggaggtgga gagaggatgt tttgcttatc cagaaaaggg       60

agtgattgct tccaggggcc tcaggggaat aaatcataga atcctggaca aggtttgaag      120

gacaggtagg atttgggtgg gtggaggagg gtgcatgggg tcagaattgt aaccgaaaac      180

tcattccagg tggatagagc                                                  200


<210>  66
<211>  209
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RNF2

<400>  66
ccaacataca gaagtcagga atgcttgaat ataaatttat tattactcta tgttctattt       60

aagttttcat gttctaaaaa tgtatcccag tttacacgtc tcatatgccc cttggcagtc      120

atcttagtca ttacctgagg tgttcgttgt aactcatata aactgagttc ccatgttttg      180

cttaatggtt gagttccgtt tgtctgcac                                        209


<210>  67
<211>  242
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  YFP (ABE)

<400>  67
cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga       60

cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg      120

catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga      180

gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa      240

gg                                                                     242


<210>  68
<211>  200
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  YFP (CBE)

<400>  68
gcgccaagat ccattcgttg ggggatccac cggtcgccac cttggtgagc aagggcgagg       60

agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca      120

agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt      180

tcatctgcac caccggcaag                                                  200


<210>  69
<211>  492
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  YFP (CBE-AIDx)

<400>  69
cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa       60

ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc      120

aagctggcta gcatgtccgt cctgacgccg ctgctgctgc ggggcttgac aggctcggcc      180

cggcggctcc cagtgccgcg cgccaagatc cattcgttgg gggatccacc ggtcgccacc      240

ttggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac      300

ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac      360

ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc      420

ctcgtgacca ccttcggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag      480

cagcacgact tc                                                          492


<210>  70
<211>  191
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCDH15 (ABE)

<400>  70
ccaagctaat gaccgtgccc aaaatctgaa tgagaggtga accaccacca ccactctcac       60

agtggatgtt ctggatggag atgacttggg tccaatgttt cttccttgtg tccttgtgcc      120

aaacactcgt gattgccgtc cactcactta tcaagctgcc atacctgagt tgagaactcc      180

ggaagaactg a                                                           191


<210>  71
<211>  173
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ABE1 (SaCas9 site)

<400>  71
acaagacctg gctgagctaa ctgtgacagc atgtggtaat tttccagccc gctggccctg       60

taaaggaaac tggaacacaa agcatagact gcggggcggg ccagcctgaa tagctgcaaa      120

caagtgcaga atatctgatg atgtcatacg cacagtttga cagatggggc tgg             173


<210>  72
<211>  210
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ABE3 (SaCas9 site)

<400>  72
gtggcaggca gattatcatt cccatcttat aaatgagtaa attgaggctc agaggagatg       60

tgccatggct taggtcctgc cgacagccag tggttaagtc agaacccgac tcaggtcaag      120

aaagcagaga ctgccggggg ttgggaaggc ggtgaactca gagatagaaa cagggtgggt      180

gggggaagga ggagctgctg aaggtgagga                                       210


<210>  73
<211>  212
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ABE4 (SaCas9 site)

<400>  73
gtgctaaaca gagagttact gctcagacat gtaataataa taaataacac atcaaataac       60

cataccattt taagctgtag tattatgaag ggaaatctgg agcaaagaga atagactgta      120

gggaaaccag ttaagaaata ggacatggag gctaggtgca gtggctcacg cctgtaatcg      180

cagcactttg ggaggctgag gcaggtggat ca                                    212


<210>  74
<211>  218
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ABE5 (SaCas9 site)

<400>  74
catcttaccc tcctcagaag tcaagcctga gcttccaaaa atgaaggaac tcaggtccag       60

ggaaactttc tgttttgtct gagggtgagg gatgagataa tgatgagtca gggcttcagg      120

gaaacgaaca tttactgatt acctaatgtg ggggtagatg tggtccctgc tctcagaaag      180

ctcaaagtgt gaatgaatta aagtcaatgt gaccaggc                              218


<210>  75
<211>  209
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ABE7 (SaCas9 site)

<400>  75
gttccaccgc cttgtttact gctcagtccc ccagaatggc aggcacgtag tagggctcag       60

taaatatttg ctgaatgaag ttatgatcga aagaatacta agcatagact ccaggataag      120

gtaagtaata tagagttcca cagtgggcag gtctatgcaa acctacccca aagtccgagg      180

aagctgagag ggcgaagaaa gaggctgag                                        209


<210>  76
<211>  225
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ABE8 (SaCas9 site)

<400>  76
catacttaaa gtaaagcaga aggaataaca gtgccccaaa ttattcacta cccctgttcc       60

taaagcccac catcttttgt acactcagag taaacaaagc atagactgag gggtacaatc      120

ctactctagt ccctttcctc atgcaacagc tatgaaatag ctggttagta ctgacagcaa      180

ttcttggcca caaacagaac cagttctata gtggaatgaa acttc                      225


<210>  77
<211>  218
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ABE9 (SaCas9 site)

<400>  77
atcatagcga gaccctgtct cagcaacaac aacaaaacgg acagtgaaga gagtatttga       60

aggagagggg aacaggcagc gtattgctta ttgctgaggg gcaaagtgaa gaccaaggat      120

agactgctgg gcttgacagc atggagggtg ctgggggcct aggcaagtgc aatattcatg      180

tggtgtcatt gggccaaagg tgtcattgga gagtactc                              218


<210>  78
<211>  247
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ABE11 (SaCas9 site)

<400>  78
gaggactact gcatcttatt gcctgcaaga aaataatttt aatactaaat tctggaaatg       60

acatgctact cacttgcttc ctccatggaa aaattatatc ttgagataca aggacaggca      120

gcatagactg tgggtataga atgtggctgg ggctgcatga aaagaaaagt tattgggagc      180

tagacaaaat ggagttcgag ttcttgtctt tggcaaaatt gctgtctctt tctaggcctt      240

catttcc                                                                247


<210>  79
<211>  230
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HEKsite4 (SaCas9 site)

<400>  79
ttcaagatgg ctgacaaagg ccgggctggg tggaaggaag ggaggaaggg cgaggcagag       60

ggtccaaagc aggatgacag gcaggggcac cgcggcgccc cggtggcact gcggctggag      120

gtgggggtta aagcggagac tctggtgctg tgtgactaca gtgggggccc tgccctctct      180

gagcccccgc ctccaggcct gtgtgtgtgt ctccgttcgg gttgaaagga                 230


<210>  80
<211>  253
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ABE17 (SaCas9 site)

<400>  80
ggacatttcc accgcaaaat ggcccctctg gtggtggccc cttcctgcag cgccggctca       60

cctcacggcc ccgcccttcc cctgccagcc tagcgttgac ccgaccccaa aggccaggct      120

gtaaatgtca ccgggaggat tgggtgtctg ggcgcctcgg ggaacctgcc cttctcccca      180

ttccgtcttc cggaaaccag atctcccacc gcaccctggt ctgaggttaa atatagctgc      240

tgacctttct gta                                                         253


<210>  81
<211>  289
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ABE18 (SaCas9 site)

<400>  81
tgtgtatggt gagaggtagg gatctagttt cattcttcta catggttgta aactgtagtc       60

accctactga tctatcaaac actgggtcat acttcttcta ttaaactgta tgtttgtacc      120

catcaatcaa cttctctttc tctcccccac ccctctactc ttcctgacct ctagtaacca      180

gcagtctatt ctctatcttc atgagatcca cttttttagt ttccacatgt gagtgagaac      240

atttgatact tgtttttttt cacatttgtt atactcctgg gctgaagtg                  289


<210>  82
<211>  276
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ABE19 (SaCas9 site)

<400>  82
ctgcacctag cctccatgtc ctatttctta actggtttcc ctacagtcta ttctctttgc       60

tccagatttc ccttcataat actacagctt aaaatggtat ggttatttga tgtgttattt      120

attattatta catgtctgag cagtaactct ctgtttagca cattacctga cactaaataa      180

atgtttagca attatagtaa ttatatgtgg aataaatgaa agaaaagcag ctgaacactt      240

aaggagataa gaaaccatgt ctctggatgc cacagc                                276


<210>  83
<211>  153
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ABE21 (SaCas9 site)

<400>  83
ccctgttcct aaagcccacc atcttttgta cactcagagt aaacaaagca tagactgagg       60

ggtacaatcc tactctagtc cctttcctca tgcaacagct atgaaatagc tggttagtac      120

tgacagcaat tcttggccac aaacagaacc agt                                   153


<210>  84
<211>  184
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ABE27 (SaCas9 site)

<400>  84
cgcgggctga agtagatcaa gtccccaggg agctcaagcc tgattccaag gagattgcca       60

atattttagg agggagtaaa cattttctga agttttttgt tgtctaaagc caaaaaatgc      120

acttgcgcct cctgagagga caaagaggaa gagagacggg gagaggggac aaaggagcag      180

agac                                                                   184


<210>  85
<211>  217
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNAJB  (mRNA Off-Target Site 1)

<400>  85
gcgctaccac ccggacaaga acaaggagcc cggcgccgag gagaagttca aggagatcgc       60

tgaggcctac gacgtgctca gcgacccgcg caagcgcgag atcttcgacc gctacgggga      120

ggaaggccta aaggggagtg gccccagtgg cggtagcggc ggtggtgcca atggtacctc      180

tttcagctac acattccatg gagaccctca tgccatg                               217


<210>  86
<211>  226
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MTA2  (mRNA Off-Target Site 2)

<400>  86
gggattcgtt caagctcaca gccagcagcc aagcgtcaga aactaaaccc agctgatgcc       60

cccaatcctg tggtgtttgt ggccacaaag gataccaggg ccctacggaa ggctctgacc      120

catctggaaa tgcggcgagc tgctcgccga cccaacttgc ccctgaaggt gaagccaacg      180

ctgattgcag tgcggccccc tgtccctcta cctgcaccct cacatc                     226


<210>  87
<211>  236
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PTBP2  (mRNA Off-Target Site 3)

<400>  87
agattttggt aattccccat tgcatcgttt taagaaacct ggatccaaaa attttcaaaa       60

catttttcct ccttctgcca cccttcacct atctaatatc cctccatcag tagcagaaga      120

ggatctacga acactgttcg ctaacactgg gggcactgtg aaagcattta agttttttca      180

aagagatcac aaaatggctc ttcttcagat ggcaacagtg gaagaagcta ttcagg          236


<210>  88
<211>  220
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SAP30BP  (mRNA Off-Target Site 4)

<400>  88
cagaaccccc tggcagatgt tcaaatcact tgcaagacaa gatccagaag ctttatgaac       60

gaaagataaa ggagggaatg gatatgaact acattatcca aaggaagaaa gaatttcgga      120

accctagcat ctacgagaag ctgatccagt tctgtgccat tgacgagctt ggcaccaact      180

acccaaagga tatgtttgat ccccatggct ggtctgagga                            220


<210>  89
<211>  210
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LCMT1 (mRNA Off-Target Site 5)

<400>  89
attgccaaca ctcctgatag ctgaatgtgt gctggtttac atgactccag agcagtccgc       60

aaacctcctg aagtgggcag ccaacagttt tgagagagcc atgttcataa actacgaaca      120

ggtgaacatg ggtgatcggt ttgggcagat catgattgaa aacctgcgga gacgccagtg      180

tgacctggcg ggagtggaga cctgcaagtc                                       210


<210>  90
<211>  181
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SCAP  (mRNA Off-Target Site 6)

<400>  90
agcagacctg aacaagcgac tgccccctga ggcctgcctg ccctcagcca agccagtggg       60

gcagccaacg cgctacgagc ggcagctggc tgtgaggccg tccacacccc acaccatcac      120

gttgcagccg tcttccttcc gaaacctgcg gctccccaag aggctgcgtg ttgtctactt      180

c                                                                      181


<210>  91
<211>  249
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gDNA Off-Target Site 1

<400>  91
caggctgcca accatactca agactaatgc aaaatttaag cagggttgag ttttctggct       60

gaaaatataa taacataggg tcaggatgat ataaaagctt agacttctgc aaacgcactc      120

cattttgtct ataacctttc aagattgctt tagttatcta ttaccatgta acaaaccact      180

aactcttagt gataaaacaa aataactatt tttaatctct cattttcctg tggtttgact      240

tgaatcagc                                                              249


<210>  92
<211>  217
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gDNA Off-Target Site 2

<400>  92
cctgcttaca ggacttgtgt ccaaaggcag actccatttt gtctcttacc tgcatccccc       60

aaaatgatga tgacaccata tactctgtgt taacctgatg agccactcag agccccacat      120

gaggtcacag ccttatgttc tccaggacct ggccaatcaa tcacaggttc atatagtcct      180

ctgagtcatt aagtcacctc cactgcccca ttttcag                               217


<210>  93
<211>  215
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gDNA Off-Target Site 3

<400>  93
gtgcctcacc ttgctcatcc tttaccatgt gacaaggaaa acaacagcat gtgtcagtgg       60

agatccaggc tcagagccat caaggaggtc cagccatgtt ctcagtgcag atgtgatacc      120

ctaccttgtt ttaatctgaa ttgactctcc cttagctgag agagccagac aaactccatt      180

ttggctcctt cacttgtagc cccttgcagc ctctt                                 215


<210>  94
<211>  250
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gDNA Off-Target Site 4

<400>  94
acctctccca ctccaggtca tttcacatca actctttctg cctccctctt gtaagtgtga       60

taccctacct tgttttaacc tgaattgact gtcccttagc tgagagagcc agacacactc      120

cattttggct ccttcacttg cagcccctta cccacccccc ttcctcaagg acttaacttg      180

tgcaagctga ctcccagcac atcaaagaat gcaattaatg gataagatac tgtggcaagc      240

tatatccgca                                                             250


<210>  95
<211>  238
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gDNA Off-Target Site 5

<400>  95
acctcttcga gtgggcagtg agttcaggca ggggtcactc accaggcgcc ctggcccctg       60

tccagctgca caggccctgg agggaaccca gagaaaacgg agtcagcctc tcaaccactc      120

ctccgcccag tgaggccgag gggctgggag gagcggctgg tccccgcccc atgagtcacc      180

cttgcatggg aaggaggggc cggtgttagg ctgggctgtc acgacctcga ccgtcttt        238


<210>  96
<211>  233
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gDNA Off-Target Site 6

<400>  96
ctgcaagctc aaaaccctga tatgtaaatc agaatggcca atttgttaac ctacagcagc       60

acataaaaac atcaaatgta caatgtatcg tttacatctg atcagtattt atctccttat      120

aggtaagcta cataaaaagg agtttgcttg gtgagcaatg gtggagtgag gctcatgatt      180

tgagtatttc aagatgtcaa ctaagggcac ctcaaccaaa tgcacctcat gcc             233


<210>  97
<211>  248
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gDNA Off-Target Site 7

<400>  97
ggaaaagcct catggcagaa tgaaatggaa gtagatttgg tcatttgtaa gtttccttag       60

agccctgaca tcctatggct tgatgattct aagtagagat ataagagact gttttaaaca      120

ttttacttaa agaagctttg acttccttgt ttctcaaact atagatgaaa ggattcaaca      180

tggggatgac tactgtgtaa gacagacacc actttgtctt gatccattga gtagctggag      240

ctgggacg                                                               248


<210>  98
<211>  226
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gDNA Off-Target Site 8

<400>  98
tcttgtgcca ctgtgcatga ttacagaatt acacaaaacc tccacaaagg attacaatac       60

aaacaccttt atatctgcca gatatataat atttaggaag gatttgtata gggtgctatg      120

agtggatcaa tgattgctgt agaaatgaag tttatgggga gctagataat gcggagttag      180

cttagctaat gggaatgtgt agataggatt ccaggaaggg gaagca                     226


<210>  99
<211>  200
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gDNA Off-Target Site 9

<400>  99
ttgttctgca ctttttgttg gaataaagaa ttccaaggct aactacatat agcctagctc       60

cctcatttta cagatgagca aaaagagacc agagaaaata agtccgatca accagattaa      120

ataatacagt aaatgaaaac ttggattaga atctagtttt cctaattatc tagtactcta      180

tgccctgcac aatactccct                                                  200


<210>  100
<211>  200
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gDNA Off-Target Site 10

<400>  100
ttcatgagga atttgcaaca cagtgtgttt attacataag ttaagctttt tcctacttaa       60

ggcatttaaa aacacactac atctgatcta gctccccttt aaactcttcc tacatccttt      120

ttctttaccc tgttcacata aatacattgc tacccatttc ccaacggcct taatctgata      180

cgacattctg cccacaagag                                                  200


<210>  101
<211>  219
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gDNA Off-Target Site 11

<400>  101
cctgggagtt cctccagacc tgctgccgga gccaagctgg gaacccactg ccctttcctt       60

gccaagccaa ggctgtatgc tgggcattgt cggggaggta gaggcaccct atgggccagg      120

tggggacact ctaagccata cccctttccc tacaggcctg gctacagcaa tatggctacc      180

tgcctcccgg ggacctacgt acccacacac agcgctcac                             219


<210>  102
<211>  208
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gDNA Off-Target Site 12

<400>  102
acttgggaaa agtaaacact caaaaataat cttaggaagt ttgaaaatat tttcatggta       60

gtaatctgat cactagggtt acattctttg atatcctacc tttagcatgt cataggtttt      120

aggaacaggg gaacggcctc caacaaaatc attttcaacc aagtgtaggt tgtagacata      180

ctcaggaaca ctgctggttc ggtgaaga                                         208


<210>  103
<211>  205
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gDNA Off-Target Site 13

<400>  103
aagctgcggt gttgaatggt gtgtcttaga cccgggtgcc tagtgtggct cggtgcctta       60

caatgaattt ggggaaagag ttgtcaaacg aaacccatgt ttctaatgac cagcaggtaa      120

gtagttccgg ctttttcttt tcatttaatc agtggtagga aagcgtggca ccctggctat      180

cctgttttag cgagtggcag ctgtg                                            205


<210>  104
<211>  246
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gDNA Off-Target Site 14

<400>  104
cggaaatctt caccgaacca gcagtgttcc tgagtatgtc tacaacctac acttggttga       60

aaatgatttt gttggaggcc gttcccctgt tcctaaaacc tattacatgc taaaggctgg      120

cacaactgcc acttatgaag gtcgctgggg aagaggaaca gcacagtaca gctcccagaa      180

gtccgtggaa gaaaggtatt tgaggcatcc tctgaggaga ctggagattt ctcctgacag      240

cagccc                                                                 246


<210>  105
<211>  246
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gDNA Off-Target Site 15

<400>  105
ttggcatttc caaataccct ctttttacat ctaatttcaa taaattccgt actccattaa       60

aagagattaa tgtatgaggc cttaggaaaa ggggtagggc ttaaacatct ctacatatac      120

ctgaataact tactagagtg ccttatacaa agaagttcaa gaaatgttag tctcctccct      180

tctcagtaga aaagcaaaat cctatttact actagaaatg ggcttacttt cctggtccag      240

cattca                                                                 246


<210>  106
<211>  228
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gDNA Off-Target Site 16

<400>  106
cttcctcatg ctgcccttct atagtcaaac cctacccctg ctcttaaacc ctggtcatca       60

ctgatctctt atccttctcc atagttttgt cttttccaga atatcataga aatacaatca      120

tgcagaatgt aaacttttaa gattgtcttc tttcactcag tatcatgctt ttgagattta      180

ttcatgttct gtgtataaat cattcattgt ttatattgct gctgagca                   228


<210>  107
<211>  218
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gDNA Off-Target Site 17

<400>  107
tgtccaatga caggagcatg acaacattcc acctattcat tcactcactc atcctgcatt       60

gagtgagtga ctgctgtatg ccagtgccga catgcaagac ttctcaaaat ttctttcagt      120

tctaacgttc tcaggtttta gcaagagggg tattgttttg ccccaaaaca ggccatctct      180

cacattttca gactggtatc catttgtggc ttttgtgc                              218


<210>  108
<211>  226
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gDNA Off-Target Site 18

<400>  108
gcccctgagc cattaaaacc cagactagcc cagcactggt gcgttcttct ctgtccccgt       60

cctgaacctg ccaggctttc cctgaggtcc ccaggcacca ggggattatt gtcaggcccc      120

tcactgtcgt cctgggccct cggaatcatg cctctgtgcc cagagtggtg ctttggaaga      180

ggggtatggc cttgtcactg gggtccttca tggccctcac ctgttt                     226


<210>  109
<211>  245
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gDNA Off-Target Site 19

<400>  109
cagagactcg ggggactgaa gtctctgaca tgaagcgctt cctgcagggt ataagcattt       60

ggaacacggg taaggcctat gggctaaaat ctccagccag cagcggttag aaattcaatg      120

ttgtccccaa gcactttaga tctcctgagt gttttgaatt ccagtgggaa gggagttctg      180

ataaaaggca gctgagtttg gaaggattgt agcctcagga tggctcctct gccttttgac      240

ccatg                                                                  245


<210>  110
<211>  200
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gDNA Off-Target Site 20

<400>  110
aggcccatat gtgctccaga gttgttctcc agtaaggaga gcatcaccgt gcttcagaaa       60

ctggggtaga gcttgcactt tatgtagtta tttaattact ccaacaccat ataaagctct      120

ctttctctga tgaatttcct ttgtaccctt gtcaaaaatg aattgactat gtatatgcag      180

ggctatttct aaactctgtt                                                  200


<210>  111
<211>  223
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gDNA Off-Target Site 21

<400>  111
ccctgggcta tttttgtcca ctgtacctac tgttgttggg acagtcctca ttccagctta       60

acagtgggaa aactaaattt agcccagtcc aggaactgga cagacaccac ttgtttggcc      120

cttttcagtt aaaactatgg gtccagtaca gttcaaatta tagatattag ggcttcttcc      180

ccttgggaaa cttgctttac agctgtggct gagaatatgg cca                        223


<210>  112
<211>  227
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gDNA Off-Target Site 22

<400>  112
agtcccgaca ggctttccat ttgtttccaa tggctttatt ccacatgact ttagtaaacc       60

gtactcaatc aatttctgac cttggtactt tgaagcaaca cttgtataca gatattaagg      120

gaagagggaa gttaagggag gaaaatcgaa ggtcaaactg tgtgtcaaac aaggtgctgg      180

gtcctaattt tatttttagt tgcctcttga atcgctcaag cccatct                    227


<210>  113
<211>  200
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gDNA Off-Target Site 23

<400>  113
tgcccttatg aagagaagga ggacaagcct tattttctca tcaatctgca gactgatcct       60

agtgacaatc tgctgtctgt cctagagact ccagagtagc tccaaaccag tgcagccttg      120

gatgagagta gaccaagccc tccctgtctc agaatctctc tagtccttaa ggaacactat      180

ggattggctt agggacccaa                                                  200


<210>  114
<211>  229
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gDNA Off-Target Site 24

<400>  114
tgaaagctag gcatgggtga aaactacact tctttacatc ttttcaggac ttaggtatct       60

tttatgttgg ctgactttta tgtggagcat caaccctgtg tgaggtgcta tggaaggtgg      120

ggatagtggg agaagcaatg taggataatg gctaagaaca cagactccag agccgactgt      180

gtgggtctca gctgcacctt cactaaatct ttctgggacc ttgggaatg                  229


<210>  115
<211>  213
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gDNA Off-Target Site 25

<400>  115
tgagcatttc tctcaggtgt ccatgaattt aatgaaaatt acttaatttt tattataatt       60

tcaataataa tataagaaat atcttatttg ttatgaagat aaaacttctc ttttgccctg      120

aaaacctgca aacaagggga gtttcacgga ataagagata aatttttttt tattagtgtt      180

ttttgaaata acttccaagg atgtattcct ggg                                   213


<210>  116
<211>  229
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gDNA Off-Target Site 26

<400>  116
gccaggaata atgggttgtg aaccctaaca ttcctgagaa gttagacagc acctgtgtgc       60

agttattcaa gatatctttt tttttaatta ttttacttta agttttaggg tacatgtgca      120

caacgtgcag gtttgttaca tatgtataca tgtgacatgt tggtgtgctg cacccattaa      180

ctcgtcattt acattaggta tatctcctaa tgctatccct ccccactcc                  229


<210>  117
<211>  208
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gDNA Off-Target Site 27

<400>  117
gaatcttggg caccatggaa actgatatcc ttgaacacgc agaaacttgg gttcaagttt       60

tggcaaatct ctactgatta gcagcaaaca agttgtgttt ctcatgtcac ttttgttgtt      120

tattgtttat acaatagtct gccttttctc ttctctctcc tttttccctc ctatgggtgg      180

ctgctttcct catcaaggag attggtca                                         208


<210>  118
<211>  210
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gDNA Off-Target Site 28

<400>  118
gcccagaccc ttcagacaga acttctttgg cagtatttaa aagccatttg aatgatgcca       60

ggactagcta aacttcgttt ctgaacagcg gcaaacacct ggtgttttaa aagtaacagt      120

ctaatcacat aactagggct gagtgatctg caagctggcc ttgacttgtc tagaaagcac      180

aatagaaata ggagttggga tcagaatgcc                                       210


<210>  119
<211>  231
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gDNA Off-Target Site 29

<400>  119
tggggccact cagagttgat gtacacacta gtgtctaatt tctgcctgag aatagagtaa       60

gacatagtct gagtagctgc agacaaaaga ggcctaggca tggctacact aggaggactg      120

tgcacccaac attcactagg gggaaaggaa gcatgaccat ttctcatgga ccagtgatag      180

accaggtata agatagagct gtgtatgagc cagagggttg cctgaagagg c               231


<210>  120
<211>  222
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gDNA Off-Target Site 30

<400>  120
ttccagcctc tgacaataag cactttttcc tctatgacat taatttactc cttaaagttt       60

tctcttcagc tataatctta aggtaaatgg gaaatagaca taagtgcatt taaatttata      120

atgcactgat tctcttgtga aggtagtctt gtaatgagga aggaattcaa aacagaactt      180

gtttgcagct ctgcaaatgt gcaagaaagc tacaaacctg cc                         222


<210>  121
<211>  5291
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PX552-CMV-mCherry-U6-SpCas9

<400>  121
gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag       60

ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga      120

aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat      180

atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga      240

cgaaacaccg gaagagcgag ctcttctgtt ttagagctag aaatagcaag ttaaaataag      300

gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt ctagactgca      360

gagggccctg cgtatgagtg caagtgggtt ttaggaccag gatgaggcgg ggtgggggtg      420

cctacctgac gaccgacccc gacccactgg acaagcaccc aacccccatt ccccaaattg      480

cgcatcccct atcagagagg gggaggggaa acaggatgcg gcgaggcgcg tgcgcactgc      540

cagcttcagc accgcggaca gtgccttcgc ccccgcctgg cggcgcgcgc caccgccgcc      600

tcagcactga aggcgcgctg acgtcactcg ccggtccccc gcaaactccc cttcccggcc      660

accttggtcg cgtccgcgcc gccgccggcc cagccggacc gcaccacgcg aggcgcgaga      720

taggggggca cgggcgcgac catctgcgct gcggcgccgg cgactcagcg ctgcctcagt      780

ctgcggtggg cagcggagga gtcgtgtcgt gcctgagagc gcagtcgaga aggtaccgga      840

tcctctagag tcgacatggt gagcaagggc gaggaggata acatggccat catcaaggag      900

ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag      960

ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag     1020

ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag     1080

gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc     1140

ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac     1200

tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc     1260

tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg     1320

taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc     1380

ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc     1440

ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc     1500

gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac     1560

aagtagtccg gactcagatc tcgagaggag gaggaggaga cagacagcag gatgccccac     1620

ctcgacagcc ccggcagctc ccagccgaga cgctccttcc tctcaagggt gatcagggca     1680

gcgctaccgt tgcagctgct tctgctgctg ctgctgctcc tggcctgcct gctacctgcc     1740

tctgaagatg actacagctg cacccaggcc aacaactttg cccgatcctt ctaccccatg     1800

ctgcggtaca ccaacgggcc acctcccacc taggaattcg atatcaagct tatcgatacc     1860

gagcgctgct cgagagatct acgggtggca tccctgtgac ccctccccag tgcctctcct     1920

ggccctggaa gttgccactc cagtgcccac cagccttgtc ctaataaaat taagttgcat     1980

cattttgtct gactaggtgt ccttctataa tattatgggg tggagggggg tggtatggag     2040

caaggggcaa gttgggaaga caacctgtag ggcctgcggg gtctattggg aaccaagctg     2100

gagtgcagtg gcacaatctt ggctcactgc aatctccgcc tcctgggttc aagcgattct     2160

cctgcctcag cctcccgagt tgttgggatt ccaggcatgc atgaccaggc tcagctaatt     2220

tttgtttttt tggtagagac ggggtttcac catattggcc aggctggtct ccaactccta     2280

atctcaggtg atctacccac cttggcctcc caaattgctg ggattacagg cgtgaaccac     2340

tgctcccttc cctgtccttc tgattttgta ggtaaccacg tgcggaccga gcggccgcag     2400

gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc     2460

gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga     2520

gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt     2580

atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg     2640

cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg     2700

ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc     2760

taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa     2820

aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc     2880

ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac     2940

tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt tcggcctatt     3000

ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt     3060

ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc     3120

cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg     3180

cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat     3240

caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca     3300

tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc     3360

ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct     3420

gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg     3480

cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg     3540

tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc     3600

tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca     3660

cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac     3720

tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa     3780

agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg     3840

ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt     3900

ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg     3960

aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc     4020

gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga     4080

tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta     4140

ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc     4200

cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg     4260

atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt     4320

cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa     4380

ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt     4440

cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt     4500

ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt     4560

tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga     4620

taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag     4680

caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata     4740

agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg     4800

gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga     4860

gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca     4920

ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa     4980

acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt     5040

tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac     5100

ggttcctggc cttttgctgg ccttttgctc acatgtcctg caggcagctg cgcgctcgct     5160

cgctcactga ggccgcccgg gcaaagcccg ggcgtcgggc gacctttggt cgcccggcct     5220

cagtgagcga gcgagcgcgc agagagggag tggccaactc catcactagg ggttcctgcg     5280

gccgcacgcg t                                                          5291


<210>  122
<211>  5312
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PX552-CMV-mCherry-U6-SaCas9

<400>  122
atgtcctgca ggcagctgcg cgctcgctcg ctcactgagg ccgcccgggc gtcgggcgac       60

ctttggtcgc ccggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaactccat      120

cactaggggt tcctgcggcc gcacgcgtga gggcctattt cccatgattc cttcatattt      180

gcatatacga tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa      240

gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt      300

aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt      360

cttggcttta tatatcttgt ggaaaggacg aaacaccgga agagcgagct cttctgtttt      420

agtactctgg aaacagaatc tactaaaaca aggcaaaatg ccgtgtttat ctcgtcaact      480

tgttggcgag atttttgggc cccgttacat aacttacggt aaatggcccg cctggctgac      540

cgcccaacga cccccgccca ttgacgtcaa tagtaacgcc aatagggact ttccattgac      600

gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata      660

tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattgtgccc      720

agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta      780

ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac      840

ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc      900

aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc      960

gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag aggtaccgga     1020

tcctctagag tcgacatggt gagcaagggc gaggaggata acatggccat catcaaggag     1080

ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag     1140

ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag     1200

ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag     1260

gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc     1320

ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac     1380

tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc     1440

tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg     1500

taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc     1560

ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc     1620

ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc     1680

gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac     1740

aagtagtccg gactcagatc tcgagaggag gaggaggaga cagacagcag gatgccccac     1800

ctcgacagcc ccggcagctc ccagccgaga cgctccttcc tctcaagggt gatcagggca     1860

gcgctaccgt tgcagctgct tctgctgctg ctgctgctcc tggcctgcct gctacctgcc     1920

tctgaagatg actacagctg cacccaggcc aacaactttg cccgatcctt ctaccccatg     1980

ctgcggtaca ccaacgggcc acctcccacc taggaattcg atatcaagct tatcgatacc     2040

gagcgctgct cgagagatct acgggtggca tccctgtgac ccctccccag tgcctctcct     2100

ggccctggaa gttgccactc cagtgcccac cagccttgtc ctaataaaat taagttgcat     2160

cattttgtct gactaggtgt ccttctataa tattatgggg tggagggggg tggtatggag     2220

caaggggcaa gttgggaaga caacctgtag ggcctgcggg gtctattggg aaccaagctg     2280

gagtgcagtg gcacaatctt ggctcactgc aatctccgcc tcctgggttc aagcgattct     2340

cctgcctcag cctcccgagt tgttgggatt ccaggcatgc atgaccaggc tcagctaatt     2400

tttgtttttt tggtagagac ggggtttcac catattggcc aggctggtct ccaactccta     2460

atctcaggtg atctacccac cttggcctcc caaattgctg ggattacagg cgtgaaccac     2520

tgctcccttc cctgtccttc tgattttgta ggtaaccacg tgcggaccga gcggccgcag     2580

gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc     2640

gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga     2700

gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt     2760

atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg     2820

cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg     2880

ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc     2940

taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa     3000

aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc     3060

ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac     3120

tcaactctat ctcgggctat tcttttgatt tataagggat tttgccgatt tcggtctatt     3180

ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt     3240

ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc     3300

cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg     3360

cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat     3420

caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca     3480

tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc     3540

ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct     3600

gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg     3660

cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg     3720

tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc     3780

tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca     3840

cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac     3900

tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa     3960

agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg     4020

ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt     4080

ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg     4140

aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc     4200

gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga     4260

tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta     4320

ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc     4380

cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg     4440

atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt     4500

cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa     4560

ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt     4620

cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt     4680

ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt     4740

tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga     4800

taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag     4860

caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata     4920

agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg     4980

gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga     5040

gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca     5100

ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa     5160

acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt     5220

tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac     5280

ggttcctggc cttttgctgg ccttttgctc ac                                   5312


<210>  123
<211>  4986
<212>  DNA
<213>  Artificial sequence

<220>
<223>  ABEMax nSaCas9

<400>  123
atgaaacgga cagccgacgg aagcgagttc gagtcaccaa agaagaagcg gaaagtctct       60

gaagtcgagt ttagccacga gtattggatg aggcacgcac tgaccctggc aaagcgagca      120

tgggatgaaa gagaagtccc cgtgggcgcc gtgctggtgc acaacaatag agtgatcgga      180

gagggatgga acaggccaat cggccgccac gaccctaccg cacacgcaga gatcatggca      240

ctgaggcagg gaggcctggt catgcagaat taccgcctga tcgatgccac cctgtatgtg      300

acactggagc catgcgtgat gtgcgcagga gcaatgatcc acagcaggat cggaagagtg      360

gtgttcggag cacgggacgc caagaccggc gcagcaggct ccctgatgga tgtgctgcac      420

caccccggca tgaaccaccg ggtggagatc acagagggaa tcctggcaga cgagtgcgcc      480

gccctgctga gcgatttctt tagaatgcgg agacaggaga tcaaggccca gaagaaggca      540

cagagctcca ccgactctgg aggatctagc ggaggatcct ctggaagcga gacaccaggc      600

acaagcgagt ccgccacacc agagagctcc ggcggctcct ccggaggatc ctctgaggtg      660

gagttttccc acgagtactg gatgagacat gccctgaccc tggccaagag ggcacgcgat      720

gagagggagg tgcctgtggg agccgtgctg gtgctgaaca atagagtgat cggcgagggc      780

tggaacagag ccatcggcct gcacgaccca acagcccatg ccgaaattat ggccctgaga      840

cagggcggcc tggtcatgca gaactacaga ctgattgacg ccaccctgta cgtgacattc      900

gagccttgcg tgatgtgcgc cggcgccatg atccactcta ggatcggccg cgtggtgttt      960

ggcgtgagga acgcaaaaac cggcgccgca ggctccctga tggacgtgct gcactacccc     1020

ggcatgaatc accgcgtcga aattaccgag ggaatcctgg cagatgaatg tgccgccctg     1080

ctgtgctatt tctttcggat gcctagacag gtgttcaatg ctcagaagaa ggcccagagc     1140

tccaccgact ccggaggatc tagcggaggc tcctctggct ctgagacacc tggcacaagc     1200

gagagcgcaa cacctgaaag cagcgggggc agcagcgggg ggtcagggaa gcgaaattac     1260

attctggggc tggccattgg cattacatca gtgggctatg gcatcattga ctacgagaca     1320

agggacgtga tcgacgccgg cgtgagactg ttcaaggagg ccaacgtgga gaacaatgag     1380

ggccggagat ccaagagggg agcaaggcgc ctgaagcgga gaaggcgcca cagaatccag     1440

agagtgaaga agctgctgtt cgattacaac ctgctgaccg accactccga gctgtctggc     1500

atcaatcctt atgaggccag agtgaagggc ctgtcccaga agctgtctga ggaggagttt     1560

agcgccgccc tgctgcacct ggcaaagagg agaggcgtgc acaacgtgaa tgaggtggag     1620

gaggacaccg gcaacgagct gtccacaaag gagcagatca gccgcaattc caaggccctg     1680

gaggagaagt atgtggccga gctgcagctg gagcggctga agaaggatgg cgaggtgagg     1740

ggctccatca atcgcttcaa gacctctgac tacgtgaagg aggccaagca gctgctgaag     1800

gtgcagaagg cctaccacca gctggatcag tcctttatcg atacatatat cgacctgctg     1860

gagacaaggc gcacatacta tgagggacca ggagagggct ctcccttcgg ctggaaggac     1920

atcaaggagt ggtacgagat gctgatgggc cactgcacct attttccaga ggagctgaga     1980

agcgtgaagt acgcctataa cgccgatctg tacaacgccc tgaatgacct gaacaacctg     2040

gtcatcacca gggatgagaa cgagaagctg gagtactatg agaagttcca gatcatcgag     2100

aacgtgttca agcagaagaa gaagcctaca ctgaagcaga tcgccaagga gatcctggtg     2160

aacgaggagg acatcaaggg ctaccgcgtg acctccacag gcaagccaga gttcaccaat     2220

ctgaaggtgt atcacgatat caaggacatc acagcccgga aggagatcat cgagaacgcc     2280

gagctgctgg atcagatcgc caagatcctg accatctatc agagctccga ggacatccag     2340

gaggagctga ccaacctgaa tagcgagctg acacaggagg agatcgagca gatcagcaat     2400

ctgaagggct acaccggcac acacaacctg agcctgaagg ccatcaatct gatcctggat     2460

gagctgtggc acacaaacga caatcagatc gccatcttta accggctgaa gctggtgcca     2520

aagaaggtgg acctgtccca gcagaaggag atcccaacca cactggtgga cgatttcatc     2580

ctgtctcccg tggtgaagcg gagcttcatc cagagcatca aagtgatcaa cgccatcatc     2640

aagaagtacg gcctgcccaa tgatatcatc atcgagctgg ccagggagaa gaactccaag     2700

gacgcccaga agatgatcaa tgagatgcag aagaggaacc gccagaccaa tgagcggatc     2760

gaggagatca tcagaaccac aggcaaggag aacgccaagt acctgatcga gaagatcaag     2820

ctgcacgata tgcaggaggg caagtgtctg tattctctgg aggccatccc tctggaggac     2880

ctgctgaaca atccattcaa ctacgaggtg gatcacatca tcccccggag cgtgagcttc     2940

gacaattctt ttaacaataa ggtgctggtg aagcaggagg agaacagcaa gaagggcaat     3000

aggacccctt tccagtacct gtctagctcc gattctaaga tcagctacga gacattcaag     3060

aagcacatcc tgaatctggc caagggcaag ggccgcatca gcaagaccaa gaaggagtac     3120

ctgctggagg agcgggacat caacagattc tccgtgcaga aggacttcat caaccggaat     3180

ctggtggaca ccagatacgc cacacgcggc ctgatgaatc tgctgcggtc ttatttcaga     3240

gtgaacaatc tggatgtgaa ggtgaagagc atcaacggcg gcttcacctc ctttctgcgg     3300

agaaagtgga agtttaagaa ggagcgcaac aagggctata agcaccacgc cgaggatgcc     3360

ctgatcatcg ccaatgccga cttcatcttt aaggagtgga agaagctgga caaggccaag     3420

aaagtgatgg agaaccagat gttcgaggag aagcaggccg agagcatgcc cgagatcgag     3480

acagagcagg agtacaagga gattttcatc acacctcacc agatcaagca catcaaggac     3540

ttcaaggact acaagtattc tcacagggtg gataagaagc ccaaccgcga gctgatcaat     3600

gacaccctgt atagcacacg gaaggacgat aagggcaata ccctgatcgt gaacaatctg     3660

aacggcctgt acgacaagga taatgacaag ctgaagaagc tgatcaacaa gtctcccgag     3720

aagctgctga tgtaccacca cgatcctcag acatatcaga agctgaagct gatcatggag     3780

cagtacggcg acgagaagaa cccactgtat aagtactatg aggagacagg caactacctg     3840

acaaagtata gcaagaagga taatggcccc gtgatcaaga agatcaagta ctatggcaac     3900

aagctgaatg cccacctgga catcaccgac gattacccta actctcgcaa taaggtggtg     3960

aagctgagcc tgaagccata ccggttcgac gtgtacctgg acaacggcgt gtataagttt     4020

gtgacagtga agaatctgga tgtgatcaag aaggagaact actatgaggt gaacagcaag     4080

tgctacgagg aggccaagaa gctgaagaag atcagcaacc aggccgagtt catcgcctct     4140

ttttacaaca atgacctgat caagatcaat ggcgagctgt atagagtgat cggcgtgaac     4200

aatgatctgc tgaacagaat cgaagtgaat atgatcgaca tcacctacag ggagtatctg     4260

gagaacatga atgataagag gccccctcgc atcatcaaga ccatcgcctc taagacacag     4320

agcatcaaga agtacagcac agacatcctg gggaacctgt atgaagtcaa gagcaagaaa     4380

catcctcaga ttatcaagaa aggctctggt ggttctccca agaagaagag gaaagtctct     4440

ggtggttcta agcgacctgc cgccacaaag aaggctggac aggctaagaa gaagaaagat     4500

tacaaagacg atgacgataa gggatccggc gcaacaaact tctctctgct gaaacaagcc     4560

ggagatgtcg aagagaatcc tggaccgatg gccaagcctt tgtctcaaga agaatccacc     4620

ctcattgaaa gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc     4680

gccagcgcag ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt     4740

actgggggac cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc     4800

aacctgactt gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga     4860

cggtgccgac aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt     4920

gatggacagc cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag     4980

ggctaa                                                                4986


<210>  124
<211>  4392
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  miniABEMax V82G nSaCas9

<400>  124
atgaaacgga cagccgacgg aagcgagttc gagtcaccaa agaagaagcg gaaagtctct       60

gaggtggagt tttcccacga gtactggatg agacatgccc tgaccctggc caagagggca      120

cgcgatgaga gggaggtgcc tgtgggagcc gtgctggtgc tgaacaatag agtgatcggc      180

gagggctgga acagagccat cggcctgcac gacccaacag cccatgccga aattatggcc      240

ctgagacagg gcggcctggt catgcagaac tacagactga ttgacgccac cctgtacggc      300

acattcgagc cttgcgtgat gtgcgccggc gccatgatcc actctaggat cggccgcgtg      360

gtgtttggcg tgaggaacgc aaaaaccggc gccgcaggct ccctgatgga cgtgctgcac      420

taccccggca tgaatcaccg cgtcgaaatt accgagggaa tcctggcaga tgaatgtgcc      480

gccctgctgt gctatttctt tcggatgcct agacaggtgt tcaatgctca gaagaaggcc      540

cagagctcca ccgactccgg aggatctagc ggaggctcct ctggctctga gacacctggc      600

acaagcgaga gcgcaacacc tgaaagcagc gggggcagca gcggggggtc agggaagcga      660

aattacattc tggggctggc cattggcatt acatcagtgg gctatggcat cattgactac      720

gagacaaggg acgtgatcga cgccggcgtg agactgttca aggaggccaa cgtggagaac      780

aatgagggcc ggagatccaa gaggggagca aggcgcctga agcggagaag gcgccacaga      840

atccagagag tgaagaagct gctgttcgat tacaacctgc tgaccgacca ctccgagctg      900

tctggcatca atccttatga ggccagagtg aagggcctgt cccagaagct gtctgaggag      960

gagtttagcg ccgccctgct gcacctggca aagaggagag gcgtgcacaa cgtgaatgag     1020

gtggaggagg acaccggcaa cgagctgtcc acaaaggagc agatcagccg caattccaag     1080

gccctggagg agaagtatgt ggccgagctg cagctggagc ggctgaagaa ggatggcgag     1140

gtgaggggct ccatcaatcg cttcaagacc tctgactacg tgaaggaggc caagcagctg     1200

ctgaaggtgc agaaggccta ccaccagctg gatcagtcct ttatcgatac atatatcgac     1260

ctgctggaga caaggcgcac atactatgag ggaccaggag agggctctcc cttcggctgg     1320

aaggacatca aggagtggta cgagatgctg atgggccact gcacctattt tccagaggag     1380

ctgagaagcg tgaagtacgc ctataacgcc gatctgtaca acgccctgaa tgacctgaac     1440

aacctggtca tcaccaggga tgagaacgag aagctggagt actatgagaa gttccagatc     1500

atcgagaacg tgttcaagca gaagaagaag cctacactga agcagatcgc caaggagatc     1560

ctggtgaacg aggaggacat caagggctac cgcgtgacct ccacaggcaa gccagagttc     1620

accaatctga aggtgtatca cgatatcaag gacatcacag cccggaagga gatcatcgag     1680

aacgccgagc tgctggatca gatcgccaag atcctgacca tctatcagag ctccgaggac     1740

atccaggagg agctgaccaa cctgaatagc gagctgacac aggaggagat cgagcagatc     1800

agcaatctga agggctacac cggcacacac aacctgagcc tgaaggccat caatctgatc     1860

ctggatgagc tgtggcacac aaacgacaat cagatcgcca tctttaaccg gctgaagctg     1920

gtgccaaaga aggtggacct gtcccagcag aaggagatcc caaccacact ggtggacgat     1980

ttcatcctgt ctcccgtggt gaagcggagc ttcatccaga gcatcaaagt gatcaacgcc     2040

atcatcaaga agtacggcct gcccaatgat atcatcatcg agctggccag ggagaagaac     2100

tccaaggacg cccagaagat gatcaatgag atgcagaaga ggaaccgcca gaccaatgag     2160

cggatcgagg agatcatcag aaccacaggc aaggagaacg ccaagtacct gatcgagaag     2220

atcaagctgc acgatatgca ggagggcaag tgtctgtatt ctctggaggc catccctctg     2280

gaggacctgc tgaacaatcc attcaactac gaggtggatc acatcatccc ccggagcgtg     2340

agcttcgaca attcttttaa caataaggtg ctggtgaagc aggaggagaa cagcaagaag     2400

ggcaatagga cccctttcca gtacctgtct agctccgatt ctaagatcag ctacgagaca     2460

ttcaagaagc acatcctgaa tctggccaag ggcaagggcc gcatcagcaa gaccaagaag     2520

gagtacctgc tggaggagcg ggacatcaac agattctccg tgcagaagga cttcatcaac     2580

cggaatctgg tggacaccag atacgccaca cgcggcctga tgaatctgct gcggtcttat     2640

ttcagagtga acaatctgga tgtgaaggtg aagagcatca acggcggctt cacctccttt     2700

ctgcggagaa agtggaagtt taagaaggag cgcaacaagg gctataagca ccacgccgag     2760

gatgccctga tcatcgccaa tgccgacttc atctttaagg agtggaagaa gctggacaag     2820

gccaagaaag tgatggagaa ccagatgttc gaggagaagc aggccgagag catgcccgag     2880

atcgagacag agcaggagta caaggagatt ttcatcacac ctcaccagat caagcacatc     2940

aaggacttca aggactacaa gtattctcac agggtggata agaagcccaa ccgcgagctg     3000

atcaatgaca ccctgtatag cacacggaag gacgataagg gcaataccct gatcgtgaac     3060

aatctgaacg gcctgtacga caaggataat gacaagctga agaagctgat caacaagtct     3120

cccgagaagc tgctgatgta ccaccacgat cctcagacat atcagaagct gaagctgatc     3180

atggagcagt acggcgacga gaagaaccca ctgtataagt actatgagga gacaggcaac     3240

tacctgacaa agtatagcaa gaaggataat ggccccgtga tcaagaagat caagtactat     3300

ggcaacaagc tgaatgccca cctggacatc accgacgatt accctaactc tcgcaataag     3360

gtggtgaagc tgagcctgaa gccataccgg ttcgacgtgt acctggacaa cggcgtgtat     3420

aagtttgtga cagtgaagaa tctggatgtg atcaagaagg agaactacta tgaggtgaac     3480

agcaagtgct acgaggaggc caagaagctg aagaagatca gcaaccaggc cgagttcatc     3540

gcctcttttt acaacaatga cctgatcaag atcaatggcg agctgtatag agtgatcggc     3600

gtgaacaatg atctgctgaa cagaatcgaa gtgaatatga tcgacatcac ctacagggag     3660

tatctggaga acatgaatga taagaggccc cctcgcatca tcaagaccat cgcctctaag     3720

acacagagca tcaagaagta cagcacagac atcctgggga acctgtatga agtcaagagc     3780

aagaaacatc ctcagattat caagaaaggc tctggtggtt ctcccaagaa gaagaggaaa     3840

gtctctggtg gttctaagcg acctgccgcc acaaagaagg ctggacaggc taagaagaag     3900

aaagattaca aagacgatga cgataaggga tccggcgcaa caaacttctc tctgctgaaa     3960

caagccggag atgtcgaaga gaatcctgga ccgatggcca agcctttgtc tcaagaagaa     4020

tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac     4080

agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat     4140

cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca     4200

gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc     4260

tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag     4320

gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg     4380

tgggagggct aa                                                         4392


<210>  125
<211>  4401
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nSaCas9 D127 miniABEMax V82G

<400>  125
atgaagcgaa attacattct ggggctggcc attggcatta catcagtggg ctatggcatc       60

attgactacg agacaaggga cgtgatcgac gccggcgtga gactgttcaa ggaggccaac      120

gtggagaaca atgagggccg gagatccaag aggggagcaa ggcgcctgaa gcggagaagg      180

cgccacagaa tccagagagt gaagaagctg ctgttcgatt acaacctgct gaccgaccac      240

tccgagctgt ctggcatcaa tccttatgag gccagagtga agggcctgtc ccagaagctg      300

tctgaggagg agtttagcgc cgccctgctg cacctggcaa agaggagagg cgtgcacaac      360

gtgaatgagg tggaggagga cccgaagaag aaacgtaagg tgggtagcag cggtagcccg      420

aaaaagaagc gcaaagtggg cagcgacgcg ctggacgatt tcgatctgga catgctgggc      480

agcgatgcgc tggatgattt cggcggtggc agctctgagg tggagttttc ccacgagtac      540

tggatgagac atgccctgac cctggccaag agggcacgcg atgagaggga ggtgcctgtg      600

ggagccgtgc tggtgctgaa caatagagtg atcggcgagg gctggaacag agccatcggc      660

ctgcacgacc caacagccca tgccgaaatt atggccctga gacagggcgg cctggtcatg      720

cagaactaca gactgattga cgccaccctg tacggcacat tcgagccttg cgtgatgtgc      780

gccggcgcca tgatccactc taggatcggc cgcgtggtgt ttggcgtgag gaacgcaaaa      840

accggcgccg caggctccct gatggacgtg ctgcactacc ccggcatgaa tcaccgcgtc      900

gaaattaccg agggaatcct ggcagatgaa tgtgccgccc tgctgtgcta tttctttcgg      960

atgcctagac aggtgttcaa tgctcagaag aaggcccaga gctccaccga cggcggtagc     1020

ggcggtagcg gtggtagcgg cggcagcggt ggtagcggtg gcagcggtgg tgacaccggc     1080

aacgagctgt ccacaaagga gcagatcagc cgcaattcca aggccctgga ggagaagtat     1140

gtggccgagc tgcagctgga gcggctgaag aaggatggcg aggtgagggg ctccatcaat     1200

cgcttcaaga cctctgacta cgtgaaggag gccaagcagc tgctgaaggt gcagaaggcc     1260

taccaccagc tggatcagtc ctttatcgat acatatatcg acctgctgga gacaaggcgc     1320

acatactatg agggaccagg agagggctct cccttcggct ggaaggacat caaggagtgg     1380

tacgagatgc tgatgggcca ctgcacctat tttccagagg agctgagaag cgtgaagtac     1440

gcctataacg ccgatctgta caacgccctg aatgacctga acaacctggt catcaccagg     1500

gatgagaacg agaagctgga gtactatgag aagttccaga tcatcgagaa cgtgttcaag     1560

cagaagaaga agcctacact gaagcagatc gccaaggaga tcctggtgaa cgaggaggac     1620

atcaagggct accgcgtgac ctccacaggc aagccagagt tcaccaatct gaaggtgtat     1680

cacgatatca aggacatcac agcccggaag gagatcatcg agaacgccga gctgctggat     1740

cagatcgcca agatcctgac catctatcag agctccgagg acatccagga ggagctgacc     1800

aacctgaata gcgagctgac acaggaggag atcgagcaga tcagcaatct gaagggctac     1860

accggcacac acaacctgag cctgaaggcc atcaatctga tcctggatga gctgtggcac     1920

acaaacgaca atcagatcgc catctttaac cggctgaagc tggtgccaaa gaaggtggac     1980

ctgtcccagc agaaggagat cccaaccaca ctggtggacg atttcatcct gtctcccgtg     2040

gtgaagcgga gcttcatcca gagcatcaaa gtgatcaacg ccatcatcaa gaagtacggc     2100

ctgcccaatg atatcatcat cgagctggcc agggagaaga actccaagga cgcccagaag     2160

atgatcaatg agatgcagaa gaggaaccgc cagaccaatg agcggatcga ggagatcatc     2220

agaaccacag gcaaggagaa cgccaagtac ctgatcgaga agatcaagct gcacgatatg     2280

caggagggca agtgtctgta ttctctggag gccatccctc tggaggacct gctgaacaat     2340

ccattcaact acgaggtgga tcacatcatc ccccggagcg tgagcttcga caattctttt     2400

aacaataagg tgctggtgaa gcaggaggag aacagcaaga agggcaatag gacccctttc     2460

cagtacctgt ctagctccga ttctaagatc agctacgaga cattcaagaa gcacatcctg     2520

aatctggcca agggcaaggg ccgcatcagc aagaccaaga aggagtacct gctggaggag     2580

cgggacatca acagattctc cgtgcagaag gacttcatca accggaatct ggtggacacc     2640

agatacgcca cacgcggcct gatgaatctg ctgcggtctt atttcagagt gaacaatctg     2700

gatgtgaagg tgaagagcat caacggcggc ttcacctcct ttctgcggag aaagtggaag     2760

tttaagaagg agcgcaacaa gggctataag caccacgccg aggatgccct gatcatcgcc     2820

aatgccgact tcatctttaa ggagtggaag aagctggaca aggccaagaa agtgatggag     2880

aaccagatgt tcgaggagaa gcaggccgag agcatgcccg agatcgagac agagcaggag     2940

tacaaggaga ttttcatcac acctcaccag atcaagcaca tcaaggactt caaggactac     3000

aagtattctc acagggtgga taagaagccc aaccgcgagc tgatcaatga caccctgtat     3060

agcacacgga aggacgataa gggcaatacc ctgatcgtga acaatctgaa cggcctgtac     3120

gacaaggata atgacaagct gaagaagctg atcaacaagt ctcccgagaa gctgctgatg     3180

taccaccacg atcctcagac atatcagaag ctgaagctga tcatggagca gtacggcgac     3240

gagaagaacc cactgtataa gtactatgag gagacaggca actacctgac aaagtatagc     3300

aagaaggata atggccccgt gatcaagaag atcaagtact atggcaacaa gctgaatgcc     3360

cacctggaca tcaccgacga ttaccctaac tctcgcaata aggtggtgaa gctgagcctg     3420

aagccatacc ggttcgacgt gtacctggac aacggcgtgt ataagtttgt gacagtgaag     3480

aatctggatg tgatcaagaa ggagaactac tatgaggtga acagcaagtg ctacgaggag     3540

gccaagaagc tgaagaagat cagcaaccag gccgagttca tcgcctcttt ttacaacaat     3600

gacctgatca agatcaatgg cgagctgtat agagtgatcg gcgtgaacaa tgatctgctg     3660

aacagaatcg aagtgaatat gatcgacatc acctacaggg agtatctgga gaacatgaat     3720

gataagaggc cccctcgcat catcaagacc atcgcctcta agacacagag catcaagaag     3780

tacagcacag acatcctggg gaacctgtat gaagtcaaga gcaagaaaca tcctcagatt     3840

atcaagaaag gctctggtgg ttctaagcga cctgccgcca caaagaaggc tggacaggct     3900

aagaagaaga aagattacaa agacgatgac gataagggat ccggcgcaac aaacttctct     3960

ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgatggccaa gcctttgtct     4020

caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct     4080

gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc     4140

aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct     4200

gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc     4260

ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc     4320

atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct     4380

ggttatgtgt gggagggcta a                                               4401


<210>  126
<211>  4401
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nSaCas9 D126 miniABEMax V82G

<400>  126
atgaagcgaa attacattct ggggctggcc attggcatta catcagtggg ctatggcatc       60

attgactacg agacaaggga cgtgatcgac gccggcgtga gactgttcaa ggaggccaac      120

gtggagaaca atgagggccg gagatccaag aggggagcaa ggcgcctgaa gcggagaagg      180

cgccacagaa tccagagagt gaagaagctg ctgttcgatt acaacctgct gaccgaccac      240

tccgagctgt ctggcatcaa tccttatgag gccagagtga agggcctgtc ccagaagctg      300

tctgaggagg agtttagcgc cgccctgctg cacctggcaa agaggagagg cgtgcacaac      360

gtgaatgagg tggaggagcc gaagaagaaa cgtaaggtgg gtagcagcgg tagcccgaaa      420

aagaagcgca aagtgggcag cgacgcgctg gacgatttcg atctggacat gctgggcagc      480

gatgcgctgg atgatttcgg cggtggcagc tctgaggtgg agttttccca cgagtactgg      540

atgagacatg ccctgaccct ggccaagagg gcacgcgatg agagggaggt gcctgtggga      600

gccgtgctgg tgctgaacaa tagagtgatc ggcgagggct ggaacagagc catcggcctg      660

cacgacccaa cagcccatgc cgaaattatg gccctgagac agggcggcct ggtcatgcag      720

aactacagac tgattgacgc caccctgtac ggcacattcg agccttgcgt gatgtgcgcc      780

ggcgccatga tccactctag gatcggccgc gtggtgtttg gcgtgaggaa cgcaaaaacc      840

ggcgccgcag gctccctgat ggacgtgctg cactaccccg gcatgaatca ccgcgtcgaa      900

attaccgagg gaatcctggc agatgaatgt gccgccctgc tgtgctattt ctttcggatg      960

cctagacagg tgttcaatgc tcagaagaag gcccagagct ccaccgacgg cggtagcggc     1020

ggtagcggtg gtagcggcgg cagcggtggt agcggtggca gcggtggtga ggacaccggc     1080

aacgagctgt ccacaaagga gcagatcagc cgcaattcca aggccctgga ggagaagtat     1140

gtggccgagc tgcagctgga gcggctgaag aaggatggcg aggtgagggg ctccatcaat     1200

cgcttcaaga cctctgacta cgtgaaggag gccaagcagc tgctgaaggt gcagaaggcc     1260

taccaccagc tggatcagtc ctttatcgat acatatatcg acctgctgga gacaaggcgc     1320

acatactatg agggaccagg agagggctct cccttcggct ggaaggacat caaggagtgg     1380

tacgagatgc tgatgggcca ctgcacctat tttccagagg agctgagaag cgtgaagtac     1440

gcctataacg ccgatctgta caacgccctg aatgacctga acaacctggt catcaccagg     1500

gatgagaacg agaagctgga gtactatgag aagttccaga tcatcgagaa cgtgttcaag     1560

cagaagaaga agcctacact gaagcagatc gccaaggaga tcctggtgaa cgaggaggac     1620

atcaagggct accgcgtgac ctccacaggc aagccagagt tcaccaatct gaaggtgtat     1680

cacgatatca aggacatcac agcccggaag gagatcatcg agaacgccga gctgctggat     1740

cagatcgcca agatcctgac catctatcag agctccgagg acatccagga ggagctgacc     1800

aacctgaata gcgagctgac acaggaggag atcgagcaga tcagcaatct gaagggctac     1860

accggcacac acaacctgag cctgaaggcc atcaatctga tcctggatga gctgtggcac     1920

acaaacgaca atcagatcgc catctttaac cggctgaagc tggtgccaaa gaaggtggac     1980

ctgtcccagc agaaggagat cccaaccaca ctggtggacg atttcatcct gtctcccgtg     2040

gtgaagcgga gcttcatcca gagcatcaaa gtgatcaacg ccatcatcaa gaagtacggc     2100

ctgcccaatg atatcatcat cgagctggcc agggagaaga actccaagga cgcccagaag     2160

atgatcaatg agatgcagaa gaggaaccgc cagaccaatg agcggatcga ggagatcatc     2220

agaaccacag gcaaggagaa cgccaagtac ctgatcgaga agatcaagct gcacgatatg     2280

caggagggca agtgtctgta ttctctggag gccatccctc tggaggacct gctgaacaat     2340

ccattcaact acgaggtgga tcacatcatc ccccggagcg tgagcttcga caattctttt     2400

aacaataagg tgctggtgaa gcaggaggag aacagcaaga agggcaatag gacccctttc     2460

cagtacctgt ctagctccga ttctaagatc agctacgaga cattcaagaa gcacatcctg     2520

aatctggcca agggcaaggg ccgcatcagc aagaccaaga aggagtacct gctggaggag     2580

cgggacatca acagattctc cgtgcagaag gacttcatca accggaatct ggtggacacc     2640

agatacgcca cacgcggcct gatgaatctg ctgcggtctt atttcagagt gaacaatctg     2700

gatgtgaagg tgaagagcat caacggcggc ttcacctcct ttctgcggag aaagtggaag     2760

tttaagaagg agcgcaacaa gggctataag caccacgccg aggatgccct gatcatcgcc     2820

aatgccgact tcatctttaa ggagtggaag aagctggaca aggccaagaa agtgatggag     2880

aaccagatgt tcgaggagaa gcaggccgag agcatgcccg agatcgagac agagcaggag     2940

tacaaggaga ttttcatcac acctcaccag atcaagcaca tcaaggactt caaggactac     3000

aagtattctc acagggtgga taagaagccc aaccgcgagc tgatcaatga caccctgtat     3060

agcacacgga aggacgataa gggcaatacc ctgatcgtga acaatctgaa cggcctgtac     3120

gacaaggata atgacaagct gaagaagctg atcaacaagt ctcccgagaa gctgctgatg     3180

taccaccacg atcctcagac atatcagaag ctgaagctga tcatggagca gtacggcgac     3240

gagaagaacc cactgtataa gtactatgag gagacaggca actacctgac aaagtatagc     3300

aagaaggata atggccccgt gatcaagaag atcaagtact atggcaacaa gctgaatgcc     3360

cacctggaca tcaccgacga ttaccctaac tctcgcaata aggtggtgaa gctgagcctg     3420

aagccatacc ggttcgacgt gtacctggac aacggcgtgt ataagtttgt gacagtgaag     3480

aatctggatg tgatcaagaa ggagaactac tatgaggtga acagcaagtg ctacgaggag     3540

gccaagaagc tgaagaagat cagcaaccag gccgagttca tcgcctcttt ttacaacaat     3600

gacctgatca agatcaatgg cgagctgtat agagtgatcg gcgtgaacaa tgatctgctg     3660

aacagaatcg aagtgaatat gatcgacatc acctacaggg agtatctgga gaacatgaat     3720

gataagaggc cccctcgcat catcaagacc atcgcctcta agacacagag catcaagaag     3780

tacagcacag acatcctggg gaacctgtat gaagtcaaga gcaagaaaca tcctcagatt     3840

atcaagaaag gctctggtgg ttctaagcga cctgccgcca caaagaaggc tggacaggct     3900

aagaagaaga aagattacaa agacgatgac gataagggat ccggcgcaac aaacttctct     3960

ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgatggccaa gcctttgtct     4020

caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct     4080

gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc     4140

aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct     4200

gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc     4260

ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc     4320

atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct     4380

ggttatgtgt gggagggcta a                                               4401


<210>  127
<211>  4401
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nSaCas9 E739 miniABEMax V82G

<400>  127
atgaagcgaa attacattct ggggctggcc attggcatta catcagtggg ctatggcatc       60

attgactacg agacaaggga cgtgatcgac gccggcgtga gactgttcaa ggaggccaac      120

gtggagaaca atgagggccg gagatccaag aggggagcaa ggcgcctgaa gcggagaagg      180

cgccacagaa tccagagagt gaagaagctg ctgttcgatt acaacctgct gaccgaccac      240

tccgagctgt ctggcatcaa tccttatgag gccagagtga agggcctgtc ccagaagctg      300

tctgaggagg agtttagcgc cgccctgctg cacctggcaa agaggagagg cgtgcacaac      360

gtgaatgagg tggaggagga caccggcaac gagctgtcca caaaggagca gatcagccgc      420

aattccaagg ccctggagga gaagtatgtg gccgagctgc agctggagcg gctgaagaag      480

gatggcgagg tgaggggctc catcaatcgc ttcaagacct ctgactacgt gaaggaggcc      540

aagcagctgc tgaaggtgca gaaggcctac caccagctgg atcagtcctt tatcgataca      600

tatatcgacc tgctggagac aaggcgcaca tactatgagg gaccaggaga gggctctccc      660

ttcggctgga aggacatcaa ggagtggtac gagatgctga tgggccactg cacctatttt      720

ccagaggagc tgagaagcgt gaagtacgcc tataacgccg atctgtacaa cgccctgaat      780

gacctgaaca acctggtcat caccagggat gagaacgaga agctggagta ctatgagaag      840

ttccagatca tcgagaacgt gttcaagcag aagaagaagc ctacactgaa gcagatcgcc      900

aaggagatcc tggtgaacga ggaggacatc aagggctacc gcgtgacctc cacaggcaag      960

ccagagttca ccaatctgaa ggtgtatcac gatatcaagg acatcacagc ccggaaggag     1020

atcatcgaga acgccgagct gctggatcag atcgccaaga tcctgaccat ctatcagagc     1080

tccgaggaca tccaggagga gctgaccaac ctgaatagcg agctgacaca ggaggagatc     1140

gagcagatca gcaatctgaa gggctacacc ggcacacaca acctgagcct gaaggccatc     1200

aatctgatcc tggatgagct gtggcacaca aacgacaatc agatcgccat ctttaaccgg     1260

ctgaagctgg tgccaaagaa ggtggacctg tcccagcaga aggagatccc aaccacactg     1320

gtggacgatt tcatcctgtc tcccgtggtg aagcggagct tcatccagag catcaaagtg     1380

atcaacgcca tcatcaagaa gtacggcctg cccaatgata tcatcatcga gctggccagg     1440

gagaagaact ccaaggacgc ccagaagatg atcaatgaga tgcagaagag gaaccgccag     1500

accaatgagc ggatcgagga gatcatcaga accacaggca aggagaacgc caagtacctg     1560

atcgagaaga tcaagctgca cgatatgcag gagggcaagt gtctgtattc tctggaggcc     1620

atccctctgg aggacctgct gaacaatcca ttcaactacg aggtggatca catcatcccc     1680

cggagcgtga gcttcgacaa ttcttttaac aataaggtgc tggtgaagca ggaggagaac     1740

agcaagaagg gcaataggac ccctttccag tacctgtcta gctccgattc taagatcagc     1800

tacgagacat tcaagaagca catcctgaat ctggccaagg gcaagggccg catcagcaag     1860

accaagaagg agtacctgct ggaggagcgg gacatcaaca gattctccgt gcagaaggac     1920

ttcatcaacc ggaatctggt ggacaccaga tacgccacac gcggcctgat gaatctgctg     1980

cggtcttatt tcagagtgaa caatctggat gtgaaggtga agagcatcaa cggcggcttc     2040

acctcctttc tgcggagaaa gtggaagttt aagaaggagc gcaacaaggg ctataagcac     2100

cacgccgagg atgccctgat catcgccaat gccgacttca tctttaagga gtggaagaag     2160

ctggacaagg ccaagaaagt gatggagaac cagatgttcg aggagaagca ggccgagccg     2220

aagaagaaac gtaaggtggg tagcagcggt agcccgaaaa agaagcgcaa agtgggcagc     2280

gacgcgctgg acgatttcga tctggacatg ctgggcagcg atgcgctgga tgatttcggc     2340

ggtggcagct ctgaggtgga gttttcccac gagtactgga tgagacatgc cctgaccctg     2400

gccaagaggg cacgcgatga gagggaggtg cctgtgggag ccgtgctggt gctgaacaat     2460

agagtgatcg gcgagggctg gaacagagcc atcggcctgc acgacccaac agcccatgcc     2520

gaaattatgg ccctgagaca gggcggcctg gtcatgcaga actacagact gattgacgcc     2580

accctgtacg gcacattcga gccttgcgtg atgtgcgccg gcgccatgat ccactctagg     2640

atcggccgcg tggtgtttgg cgtgaggaac gcaaaaaccg gcgccgcagg ctccctgatg     2700

gacgtgctgc actaccccgg catgaatcac cgcgtcgaaa ttaccgaggg aatcctggca     2760

gatgaatgtg ccgccctgct gtgctatttc tttcggatgc ctagacaggt gttcaatgct     2820

cagaagaagg cccagagctc caccgacggc ggtagcggcg gtagcggtgg tagcggcggc     2880

agcggtggta gcggtggcag cggtggtgag agcatgcccg agatcgagac agagcaggag     2940

tacaaggaga ttttcatcac acctcaccag atcaagcaca tcaaggactt caaggactac     3000

aagtattctc acagggtgga taagaagccc aaccgcgagc tgatcaatga caccctgtat     3060

agcacacgga aggacgataa gggcaatacc ctgatcgtga acaatctgaa cggcctgtac     3120

gacaaggata atgacaagct gaagaagctg atcaacaagt ctcccgagaa gctgctgatg     3180

taccaccacg atcctcagac atatcagaag ctgaagctga tcatggagca gtacggcgac     3240

gagaagaacc cactgtataa gtactatgag gagacaggca actacctgac aaagtatagc     3300

aagaaggata atggccccgt gatcaagaag atcaagtact atggcaacaa gctgaatgcc     3360

cacctggaca tcaccgacga ttaccctaac tctcgcaata aggtggtgaa gctgagcctg     3420

aagccatacc ggttcgacgt gtacctggac aacggcgtgt ataagtttgt gacagtgaag     3480

aatctggatg tgatcaagaa ggagaactac tatgaggtga acagcaagtg ctacgaggag     3540

gccaagaagc tgaagaagat cagcaaccag gccgagttca tcgcctcttt ttacaacaat     3600

gacctgatca agatcaatgg cgagctgtat agagtgatcg gcgtgaacaa tgatctgctg     3660

aacagaatcg aagtgaatat gatcgacatc acctacaggg agtatctgga gaacatgaat     3720

gataagaggc cccctcgcat catcaagacc atcgcctcta agacacagag catcaagaag     3780

tacagcacag acatcctggg gaacctgtat gaagtcaaga gcaagaaaca tcctcagatt     3840

atcaagaaag gctctggtgg ttctaagcga cctgccgcca caaagaaggc tggacaggct     3900

aagaagaaga aagattacaa agacgatgac gataagggat ccggcgcaac aaacttctct     3960

ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgatggccaa gcctttgtct     4020

caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct     4080

gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc     4140

aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct     4200

gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc     4260

ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc     4320

atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct     4380

ggttatgtgt gggagggcta a                                               4401


<210>  128
<211>  4401
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nSaCas9 E743 miniABEMax V82G

<400>  128
atgaagcgaa attacattct ggggctggcc attggcatta catcagtggg ctatggcatc       60

attgactacg agacaaggga cgtgatcgac gccggcgtga gactgttcaa ggaggccaac      120

gtggagaaca atgagggccg gagatccaag aggggagcaa ggcgcctgaa gcggagaagg      180

cgccacagaa tccagagagt gaagaagctg ctgttcgatt acaacctgct gaccgaccac      240

tccgagctgt ctggcatcaa tccttatgag gccagagtga agggcctgtc ccagaagctg      300

tctgaggagg agtttagcgc cgccctgctg cacctggcaa agaggagagg cgtgcacaac      360

gtgaatgagg tggaggagga caccggcaac gagctgtcca caaaggagca gatcagccgc      420

aattccaagg ccctggagga gaagtatgtg gccgagctgc agctggagcg gctgaagaag      480

gatggcgagg tgaggggctc catcaatcgc ttcaagacct ctgactacgt gaaggaggcc      540

aagcagctgc tgaaggtgca gaaggcctac caccagctgg atcagtcctt tatcgataca      600

tatatcgacc tgctggagac aaggcgcaca tactatgagg gaccaggaga gggctctccc      660

ttcggctgga aggacatcaa ggagtggtac gagatgctga tgggccactg cacctatttt      720

ccagaggagc tgagaagcgt gaagtacgcc tataacgccg atctgtacaa cgccctgaat      780

gacctgaaca acctggtcat caccagggat gagaacgaga agctggagta ctatgagaag      840

ttccagatca tcgagaacgt gttcaagcag aagaagaagc ctacactgaa gcagatcgcc      900

aaggagatcc tggtgaacga ggaggacatc aagggctacc gcgtgacctc cacaggcaag      960

ccagagttca ccaatctgaa ggtgtatcac gatatcaagg acatcacagc ccggaaggag     1020

atcatcgaga acgccgagct gctggatcag atcgccaaga tcctgaccat ctatcagagc     1080

tccgaggaca tccaggagga gctgaccaac ctgaatagcg agctgacaca ggaggagatc     1140

gagcagatca gcaatctgaa gggctacacc ggcacacaca acctgagcct gaaggccatc     1200

aatctgatcc tggatgagct gtggcacaca aacgacaatc agatcgccat ctttaaccgg     1260

ctgaagctgg tgccaaagaa ggtggacctg tcccagcaga aggagatccc aaccacactg     1320

gtggacgatt tcatcctgtc tcccgtggtg aagcggagct tcatccagag catcaaagtg     1380

atcaacgcca tcatcaagaa gtacggcctg cccaatgata tcatcatcga gctggccagg     1440

gagaagaact ccaaggacgc ccagaagatg atcaatgaga tgcagaagag gaaccgccag     1500

accaatgagc ggatcgagga gatcatcaga accacaggca aggagaacgc caagtacctg     1560

atcgagaaga tcaagctgca cgatatgcag gagggcaagt gtctgtattc tctggaggcc     1620

atccctctgg aggacctgct gaacaatcca ttcaactacg aggtggatca catcatcccc     1680

cggagcgtga gcttcgacaa ttcttttaac aataaggtgc tggtgaagca ggaggagaac     1740

agcaagaagg gcaataggac ccctttccag tacctgtcta gctccgattc taagatcagc     1800

tacgagacat tcaagaagca catcctgaat ctggccaagg gcaagggccg catcagcaag     1860

accaagaagg agtacctgct ggaggagcgg gacatcaaca gattctccgt gcagaaggac     1920

ttcatcaacc ggaatctggt ggacaccaga tacgccacac gcggcctgat gaatctgctg     1980

cggtcttatt tcagagtgaa caatctggat gtgaaggtga agagcatcaa cggcggcttc     2040

acctcctttc tgcggagaaa gtggaagttt aagaaggagc gcaacaaggg ctataagcac     2100

cacgccgagg atgccctgat catcgccaat gccgacttca tctttaagga gtggaagaag     2160

ctggacaagg ccaagaaagt gatggagaac cagatgttcg aggagaagca ggccgagagc     2220

atgcccgagc cgaagaagaa acgtaaggtg ggtagcagcg gtagcccgaa aaagaagcgc     2280

aaagtgggca gcgacgcgct ggacgatttc gatctggaca tgctgggcag cgatgcgctg     2340

gatgatttcg gcggtggcag ctctgaggtg gagttttccc acgagtactg gatgagacat     2400

gccctgaccc tggccaagag ggcacgcgat gagagggagg tgcctgtggg agccgtgctg     2460

gtgctgaaca atagagtgat cggcgagggc tggaacagag ccatcggcct gcacgaccca     2520

acagcccatg ccgaaattat ggccctgaga cagggcggcc tggtcatgca gaactacaga     2580

ctgattgacg ccaccctgta cggcacattc gagccttgcg tgatgtgcgc cggcgccatg     2640

atccactcta ggatcggccg cgtggtgttt ggcgtgagga acgcaaaaac cggcgccgca     2700

ggctccctga tggacgtgct gcactacccc ggcatgaatc accgcgtcga aattaccgag     2760

ggaatcctgg cagatgaatg tgccgccctg ctgtgctatt tctttcggat gcctagacag     2820

gtgttcaatg ctcagaagaa ggcccagagc tccaccgacg gcggtagcgg cggtagcggt     2880

ggtagcggcg gcagcggtgg tagcggtggc agcggtggtg agatcgagac agagcaggag     2940

tacaaggaga ttttcatcac acctcaccag atcaagcaca tcaaggactt caaggactac     3000

aagtattctc acagggtgga taagaagccc aaccgcgagc tgatcaatga caccctgtat     3060

agcacacgga aggacgataa gggcaatacc ctgatcgtga acaatctgaa cggcctgtac     3120

gacaaggata atgacaagct gaagaagctg atcaacaagt ctcccgagaa gctgctgatg     3180

taccaccacg atcctcagac atatcagaag ctgaagctga tcatggagca gtacggcgac     3240

gagaagaacc cactgtataa gtactatgag gagacaggca actacctgac aaagtatagc     3300

aagaaggata atggccccgt gatcaagaag atcaagtact atggcaacaa gctgaatgcc     3360

cacctggaca tcaccgacga ttaccctaac tctcgcaata aggtggtgaa gctgagcctg     3420

aagccatacc ggttcgacgt gtacctggac aacggcgtgt ataagtttgt gacagtgaag     3480

aatctggatg tgatcaagaa ggagaactac tatgaggtga acagcaagtg ctacgaggag     3540

gccaagaagc tgaagaagat cagcaaccag gccgagttca tcgcctcttt ttacaacaat     3600

gacctgatca agatcaatgg cgagctgtat agagtgatcg gcgtgaacaa tgatctgctg     3660

aacagaatcg aagtgaatat gatcgacatc acctacaggg agtatctgga gaacatgaat     3720

gataagaggc cccctcgcat catcaagacc atcgcctcta agacacagag catcaagaag     3780

tacagcacag acatcctggg gaacctgtat gaagtcaaga gcaagaaaca tcctcagatt     3840

atcaagaaag gctctggtgg ttctaagcga cctgccgcca caaagaaggc tggacaggct     3900

aagaagaaga aagattacaa agacgatgac gataagggat ccggcgcaac aaacttctct     3960

ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgatggccaa gcctttgtct     4020

caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct     4080

gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc     4140

aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct     4200

gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc     4260

ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc     4320

atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct     4380

ggttatgtgt gggagggcta a                                               4401


<210>  129
<211>  4401
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nSaCas9 E745 miniABEMax V82G

<400>  129
atgaagcgaa attacattct ggggctggcc attggcatta catcagtggg ctatggcatc       60

attgactacg agacaaggga cgtgatcgac gccggcgtga gactgttcaa ggaggccaac      120

gtggagaaca atgagggccg gagatccaag aggggagcaa ggcgcctgaa gcggagaagg      180

cgccacagaa tccagagagt gaagaagctg ctgttcgatt acaacctgct gaccgaccac      240

tccgagctgt ctggcatcaa tccttatgag gccagagtga agggcctgtc ccagaagctg      300

tctgaggagg agtttagcgc cgccctgctg cacctggcaa agaggagagg cgtgcacaac      360

gtgaatgagg tggaggagga caccggcaac gagctgtcca caaaggagca gatcagccgc      420

aattccaagg ccctggagga gaagtatgtg gccgagctgc agctggagcg gctgaagaag      480

gatggcgagg tgaggggctc catcaatcgc ttcaagacct ctgactacgt gaaggaggcc      540

aagcagctgc tgaaggtgca gaaggcctac caccagctgg atcagtcctt tatcgataca      600

tatatcgacc tgctggagac aaggcgcaca tactatgagg gaccaggaga gggctctccc      660

ttcggctgga aggacatcaa ggagtggtac gagatgctga tgggccactg cacctatttt      720

ccagaggagc tgagaagcgt gaagtacgcc tataacgccg atctgtacaa cgccctgaat      780

gacctgaaca acctggtcat caccagggat gagaacgaga agctggagta ctatgagaag      840

ttccagatca tcgagaacgt gttcaagcag aagaagaagc ctacactgaa gcagatcgcc      900

aaggagatcc tggtgaacga ggaggacatc aagggctacc gcgtgacctc cacaggcaag      960

ccagagttca ccaatctgaa ggtgtatcac gatatcaagg acatcacagc ccggaaggag     1020

atcatcgaga acgccgagct gctggatcag atcgccaaga tcctgaccat ctatcagagc     1080

tccgaggaca tccaggagga gctgaccaac ctgaatagcg agctgacaca ggaggagatc     1140

gagcagatca gcaatctgaa gggctacacc ggcacacaca acctgagcct gaaggccatc     1200

aatctgatcc tggatgagct gtggcacaca aacgacaatc agatcgccat ctttaaccgg     1260

ctgaagctgg tgccaaagaa ggtggacctg tcccagcaga aggagatccc aaccacactg     1320

gtggacgatt tcatcctgtc tcccgtggtg aagcggagct tcatccagag catcaaagtg     1380

atcaacgcca tcatcaagaa gtacggcctg cccaatgata tcatcatcga gctggccagg     1440

gagaagaact ccaaggacgc ccagaagatg atcaatgaga tgcagaagag gaaccgccag     1500

accaatgagc ggatcgagga gatcatcaga accacaggca aggagaacgc caagtacctg     1560

atcgagaaga tcaagctgca cgatatgcag gagggcaagt gtctgtattc tctggaggcc     1620

atccctctgg aggacctgct gaacaatcca ttcaactacg aggtggatca catcatcccc     1680

cggagcgtga gcttcgacaa ttcttttaac aataaggtgc tggtgaagca ggaggagaac     1740

agcaagaagg gcaataggac ccctttccag tacctgtcta gctccgattc taagatcagc     1800

tacgagacat tcaagaagca catcctgaat ctggccaagg gcaagggccg catcagcaag     1860

accaagaagg agtacctgct ggaggagcgg gacatcaaca gattctccgt gcagaaggac     1920

ttcatcaacc ggaatctggt ggacaccaga tacgccacac gcggcctgat gaatctgctg     1980

cggtcttatt tcagagtgaa caatctggat gtgaaggtga agagcatcaa cggcggcttc     2040

acctcctttc tgcggagaaa gtggaagttt aagaaggagc gcaacaaggg ctataagcac     2100

cacgccgagg atgccctgat catcgccaat gccgacttca tctttaagga gtggaagaag     2160

ctggacaagg ccaagaaagt gatggagaac cagatgttcg aggagaagca ggccgagagc     2220

atgcccgaga tcgagccgaa gaagaaacgt aaggtgggta gcagcggtag cccgaaaaag     2280

aagcgcaaag tgggcagcga cgcgctggac gatttcgatc tggacatgct gggcagcgat     2340

gcgctggatg atttcggcgg tggcagctct gaggtggagt tttcccacga gtactggatg     2400

agacatgccc tgaccctggc caagagggca cgcgatgaga gggaggtgcc tgtgggagcc     2460

gtgctggtgc tgaacaatag agtgatcggc gagggctgga acagagccat cggcctgcac     2520

gacccaacag cccatgccga aattatggcc ctgagacagg gcggcctggt catgcagaac     2580

tacagactga ttgacgccac cctgtacggc acattcgagc cttgcgtgat gtgcgccggc     2640

gccatgatcc actctaggat cggccgcgtg gtgtttggcg tgaggaacgc aaaaaccggc     2700

gccgcaggct ccctgatgga cgtgctgcac taccccggca tgaatcaccg cgtcgaaatt     2760

accgagggaa tcctggcaga tgaatgtgcc gccctgctgt gctatttctt tcggatgcct     2820

agacaggtgt tcaatgctca gaagaaggcc cagagctcca ccgacggcgg tagcggcggt     2880

agcggtggta gcggcggcag cggtggtagc ggtggcagcg gtggtgagac agagcaggag     2940

tacaaggaga ttttcatcac acctcaccag atcaagcaca tcaaggactt caaggactac     3000

aagtattctc acagggtgga taagaagccc aaccgcgagc tgatcaatga caccctgtat     3060

agcacacgga aggacgataa gggcaatacc ctgatcgtga acaatctgaa cggcctgtac     3120

gacaaggata atgacaagct gaagaagctg atcaacaagt ctcccgagaa gctgctgatg     3180

taccaccacg atcctcagac atatcagaag ctgaagctga tcatggagca gtacggcgac     3240

gagaagaacc cactgtataa gtactatgag gagacaggca actacctgac aaagtatagc     3300

aagaaggata atggccccgt gatcaagaag atcaagtact atggcaacaa gctgaatgcc     3360

cacctggaca tcaccgacga ttaccctaac tctcgcaata aggtggtgaa gctgagcctg     3420

aagccatacc ggttcgacgt gtacctggac aacggcgtgt ataagtttgt gacagtgaag     3480

aatctggatg tgatcaagaa ggagaactac tatgaggtga acagcaagtg ctacgaggag     3540

gccaagaagc tgaagaagat cagcaaccag gccgagttca tcgcctcttt ttacaacaat     3600

gacctgatca agatcaatgg cgagctgtat agagtgatcg gcgtgaacaa tgatctgctg     3660

aacagaatcg aagtgaatat gatcgacatc acctacaggg agtatctgga gaacatgaat     3720

gataagaggc cccctcgcat catcaagacc atcgcctcta agacacagag catcaagaag     3780

tacagcacag acatcctggg gaacctgtat gaagtcaaga gcaagaaaca tcctcagatt     3840

atcaagaaag gctctggtgg ttctaagcga cctgccgcca caaagaaggc tggacaggct     3900

aagaagaaga aagattacaa agacgatgac gataagggat ccggcgcaac aaacttctct     3960

ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgatggccaa gcctttgtct     4020

caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct     4080

gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc     4140

aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct     4200

gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc     4260

ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc     4320

atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct     4380

ggttatgtgt gggagggcta a                                               4401


<210>  130
<211>  4401
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nSaCas9 F733 miniABEMax V82G

<400>  130
atgaagcgaa attacattct ggggctggcc attggcatta catcagtggg ctatggcatc       60

attgactacg agacaaggga cgtgatcgac gccggcgtga gactgttcaa ggaggccaac      120

gtggagaaca atgagggccg gagatccaag aggggagcaa ggcgcctgaa gcggagaagg      180

cgccacagaa tccagagagt gaagaagctg ctgttcgatt acaacctgct gaccgaccac      240

tccgagctgt ctggcatcaa tccttatgag gccagagtga agggcctgtc ccagaagctg      300

tctgaggagg agtttagcgc cgccctgctg cacctggcaa agaggagagg cgtgcacaac      360

gtgaatgagg tggaggagga caccggcaac gagctgtcca caaaggagca gatcagccgc      420

aattccaagg ccctggagga gaagtatgtg gccgagctgc agctggagcg gctgaagaag      480

gatggcgagg tgaggggctc catcaatcgc ttcaagacct ctgactacgt gaaggaggcc      540

aagcagctgc tgaaggtgca gaaggcctac caccagctgg atcagtcctt tatcgataca      600

tatatcgacc tgctggagac aaggcgcaca tactatgagg gaccaggaga gggctctccc      660

ttcggctgga aggacatcaa ggagtggtac gagatgctga tgggccactg cacctatttt      720

ccagaggagc tgagaagcgt gaagtacgcc tataacgccg atctgtacaa cgccctgaat      780

gacctgaaca acctggtcat caccagggat gagaacgaga agctggagta ctatgagaag      840

ttccagatca tcgagaacgt gttcaagcag aagaagaagc ctacactgaa gcagatcgcc      900

aaggagatcc tggtgaacga ggaggacatc aagggctacc gcgtgacctc cacaggcaag      960

ccagagttca ccaatctgaa ggtgtatcac gatatcaagg acatcacagc ccggaaggag     1020

atcatcgaga acgccgagct gctggatcag atcgccaaga tcctgaccat ctatcagagc     1080

tccgaggaca tccaggagga gctgaccaac ctgaatagcg agctgacaca ggaggagatc     1140

gagcagatca gcaatctgaa gggctacacc ggcacacaca acctgagcct gaaggccatc     1200

aatctgatcc tggatgagct gtggcacaca aacgacaatc agatcgccat ctttaaccgg     1260

ctgaagctgg tgccaaagaa ggtggacctg tcccagcaga aggagatccc aaccacactg     1320

gtggacgatt tcatcctgtc tcccgtggtg aagcggagct tcatccagag catcaaagtg     1380

atcaacgcca tcatcaagaa gtacggcctg cccaatgata tcatcatcga gctggccagg     1440

gagaagaact ccaaggacgc ccagaagatg atcaatgaga tgcagaagag gaaccgccag     1500

accaatgagc ggatcgagga gatcatcaga accacaggca aggagaacgc caagtacctg     1560

atcgagaaga tcaagctgca cgatatgcag gagggcaagt gtctgtattc tctggaggcc     1620

atccctctgg aggacctgct gaacaatcca ttcaactacg aggtggatca catcatcccc     1680

cggagcgtga gcttcgacaa ttcttttaac aataaggtgc tggtgaagca ggaggagaac     1740

agcaagaagg gcaataggac ccctttccag tacctgtcta gctccgattc taagatcagc     1800

tacgagacat tcaagaagca catcctgaat ctggccaagg gcaagggccg catcagcaag     1860

accaagaagg agtacctgct ggaggagcgg gacatcaaca gattctccgt gcagaaggac     1920

ttcatcaacc ggaatctggt ggacaccaga tacgccacac gcggcctgat gaatctgctg     1980

cggtcttatt tcagagtgaa caatctggat gtgaaggtga agagcatcaa cggcggcttc     2040

acctcctttc tgcggagaaa gtggaagttt aagaaggagc gcaacaaggg ctataagcac     2100

cacgccgagg atgccctgat catcgccaat gccgacttca tctttaagga gtggaagaag     2160

ctggacaagg ccaagaaagt gatggagaac cagatgttcc cgaagaagaa acgtaaggtg     2220

ggtagcagcg gtagcccgaa aaagaagcgc aaagtgggca gcgacgcgct ggacgatttc     2280

gatctggaca tgctgggcag cgatgcgctg gatgatttcg gcggtggcag ctctgaggtg     2340

gagttttccc acgagtactg gatgagacat gccctgaccc tggccaagag ggcacgcgat     2400

gagagggagg tgcctgtggg agccgtgctg gtgctgaaca atagagtgat cggcgagggc     2460

tggaacagag ccatcggcct gcacgaccca acagcccatg ccgaaattat ggccctgaga     2520

cagggcggcc tggtcatgca gaactacaga ctgattgacg ccaccctgta cggcacattc     2580

gagccttgcg tgatgtgcgc cggcgccatg atccactcta ggatcggccg cgtggtgttt     2640

ggcgtgagga acgcaaaaac cggcgccgca ggctccctga tggacgtgct gcactacccc     2700

ggcatgaatc accgcgtcga aattaccgag ggaatcctgg cagatgaatg tgccgccctg     2760

ctgtgctatt tctttcggat gcctagacag gtgttcaatg ctcagaagaa ggcccagagc     2820

tccaccgacg gcggtagcgg cggtagcggt ggtagcggcg gcagcggtgg tagcggtggc     2880

agcggtggtt tcgaggagaa gcaggccgag agcatgcccg agatcgagac agagcaggag     2940

tacaaggaga ttttcatcac acctcaccag atcaagcaca tcaaggactt caaggactac     3000

aagtattctc acagggtgga taagaagccc aaccgcgagc tgatcaatga caccctgtat     3060

agcacacgga aggacgataa gggcaatacc ctgatcgtga acaatctgaa cggcctgtac     3120

gacaaggata atgacaagct gaagaagctg atcaacaagt ctcccgagaa gctgctgatg     3180

taccaccacg atcctcagac atatcagaag ctgaagctga tcatggagca gtacggcgac     3240

gagaagaacc cactgtataa gtactatgag gagacaggca actacctgac aaagtatagc     3300

aagaaggata atggccccgt gatcaagaag atcaagtact atggcaacaa gctgaatgcc     3360

cacctggaca tcaccgacga ttaccctaac tctcgcaata aggtggtgaa gctgagcctg     3420

aagccatacc ggttcgacgt gtacctggac aacggcgtgt ataagtttgt gacagtgaag     3480

aatctggatg tgatcaagaa ggagaactac tatgaggtga acagcaagtg ctacgaggag     3540

gccaagaagc tgaagaagat cagcaaccag gccgagttca tcgcctcttt ttacaacaat     3600

gacctgatca agatcaatgg cgagctgtat agagtgatcg gcgtgaacaa tgatctgctg     3660

aacagaatcg aagtgaatat gatcgacatc acctacaggg agtatctgga gaacatgaat     3720

gataagaggc cccctcgcat catcaagacc atcgcctcta agacacagag catcaagaag     3780

tacagcacag acatcctggg gaacctgtat gaagtcaaga gcaagaaaca tcctcagatt     3840

atcaagaaag gctctggtgg ttctaagcga cctgccgcca caaagaaggc tggacaggct     3900

aagaagaaga aagattacaa agacgatgac gataagggat ccggcgcaac aaacttctct     3960

ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgatggccaa gcctttgtct     4020

caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct     4080

gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc     4140

aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct     4200

gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc     4260

ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc     4320

atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct     4380

ggttatgtgt gggagggcta a                                               4401


<210>  131
<211>  4401
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nSaCas9 N730 miniABEMax V82G

<400>  131
atgaagcgaa attacattct ggggctggcc attggcatta catcagtggg ctatggcatc       60

attgactacg agacaaggga cgtgatcgac gccggcgtga gactgttcaa ggaggccaac      120

gtggagaaca atgagggccg gagatccaag aggggagcaa ggcgcctgaa gcggagaagg      180

cgccacagaa tccagagagt gaagaagctg ctgttcgatt acaacctgct gaccgaccac      240

tccgagctgt ctggcatcaa tccttatgag gccagagtga agggcctgtc ccagaagctg      300

tctgaggagg agtttagcgc cgccctgctg cacctggcaa agaggagagg cgtgcacaac      360

gtgaatgagg tggaggagga caccggcaac gagctgtcca caaaggagca gatcagccgc      420

aattccaagg ccctggagga gaagtatgtg gccgagctgc agctggagcg gctgaagaag      480

gatggcgagg tgaggggctc catcaatcgc ttcaagacct ctgactacgt gaaggaggcc      540

aagcagctgc tgaaggtgca gaaggcctac caccagctgg atcagtcctt tatcgataca      600

tatatcgacc tgctggagac aaggcgcaca tactatgagg gaccaggaga gggctctccc      660

ttcggctgga aggacatcaa ggagtggtac gagatgctga tgggccactg cacctatttt      720

ccagaggagc tgagaagcgt gaagtacgcc tataacgccg atctgtacaa cgccctgaat      780

gacctgaaca acctggtcat caccagggat gagaacgaga agctggagta ctatgagaag      840

ttccagatca tcgagaacgt gttcaagcag aagaagaagc ctacactgaa gcagatcgcc      900

aaggagatcc tggtgaacga ggaggacatc aagggctacc gcgtgacctc cacaggcaag      960

ccagagttca ccaatctgaa ggtgtatcac gatatcaagg acatcacagc ccggaaggag     1020

atcatcgaga acgccgagct gctggatcag atcgccaaga tcctgaccat ctatcagagc     1080

tccgaggaca tccaggagga gctgaccaac ctgaatagcg agctgacaca ggaggagatc     1140

gagcagatca gcaatctgaa gggctacacc ggcacacaca acctgagcct gaaggccatc     1200

aatctgatcc tggatgagct gtggcacaca aacgacaatc agatcgccat ctttaaccgg     1260

ctgaagctgg tgccaaagaa ggtggacctg tcccagcaga aggagatccc aaccacactg     1320

gtggacgatt tcatcctgtc tcccgtggtg aagcggagct tcatccagag catcaaagtg     1380

atcaacgcca tcatcaagaa gtacggcctg cccaatgata tcatcatcga gctggccagg     1440

gagaagaact ccaaggacgc ccagaagatg atcaatgaga tgcagaagag gaaccgccag     1500

accaatgagc ggatcgagga gatcatcaga accacaggca aggagaacgc caagtacctg     1560

atcgagaaga tcaagctgca cgatatgcag gagggcaagt gtctgtattc tctggaggcc     1620

atccctctgg aggacctgct gaacaatcca ttcaactacg aggtggatca catcatcccc     1680

cggagcgtga gcttcgacaa ttcttttaac aataaggtgc tggtgaagca ggaggagaac     1740

agcaagaagg gcaataggac ccctttccag tacctgtcta gctccgattc taagatcagc     1800

tacgagacat tcaagaagca catcctgaat ctggccaagg gcaagggccg catcagcaag     1860

accaagaagg agtacctgct ggaggagcgg gacatcaaca gattctccgt gcagaaggac     1920

ttcatcaacc ggaatctggt ggacaccaga tacgccacac gcggcctgat gaatctgctg     1980

cggtcttatt tcagagtgaa caatctggat gtgaaggtga agagcatcaa cggcggcttc     2040

acctcctttc tgcggagaaa gtggaagttt aagaaggagc gcaacaaggg ctataagcac     2100

cacgccgagg atgccctgat catcgccaat gccgacttca tctttaagga gtggaagaag     2160

ctggacaagg ccaagaaagt gatggagaac ccgaagaaga aacgtaaggt gggtagcagc     2220

ggtagcccga aaaagaagcg caaagtgggc agcgacgcgc tggacgattt cgatctggac     2280

atgctgggca gcgatgcgct ggatgatttc ggcggtggca gctctgaggt ggagttttcc     2340

cacgagtact ggatgagaca tgccctgacc ctggccaaga gggcacgcga tgagagggag     2400

gtgcctgtgg gagccgtgct ggtgctgaac aatagagtga tcggcgaggg ctggaacaga     2460

gccatcggcc tgcacgaccc aacagcccat gccgaaatta tggccctgag acagggcggc     2520

ctggtcatgc agaactacag actgattgac gccaccctgt acggcacatt cgagccttgc     2580

gtgatgtgcg ccggcgccat gatccactct aggatcggcc gcgtggtgtt tggcgtgagg     2640

aacgcaaaaa ccggcgccgc aggctccctg atggacgtgc tgcactaccc cggcatgaat     2700

caccgcgtcg aaattaccga gggaatcctg gcagatgaat gtgccgccct gctgtgctat     2760

ttctttcgga tgcctagaca ggtgttcaat gctcagaaga aggcccagag ctccaccgac     2820

ggcggtagcg gcggtagcgg tggtagcggc ggcagcggtg gtagcggtgg cagcggtggt     2880

aaccagatgt tcgaggagaa gcaggccgag agcatgcccg agatcgagac agagcaggag     2940

tacaaggaga ttttcatcac acctcaccag atcaagcaca tcaaggactt caaggactac     3000

aagtattctc acagggtgga taagaagccc aaccgcgagc tgatcaatga caccctgtat     3060

agcacacgga aggacgataa gggcaatacc ctgatcgtga acaatctgaa cggcctgtac     3120

gacaaggata atgacaagct gaagaagctg atcaacaagt ctcccgagaa gctgctgatg     3180

taccaccacg atcctcagac atatcagaag ctgaagctga tcatggagca gtacggcgac     3240

gagaagaacc cactgtataa gtactatgag gagacaggca actacctgac aaagtatagc     3300

aagaaggata atggccccgt gatcaagaag atcaagtact atggcaacaa gctgaatgcc     3360

cacctggaca tcaccgacga ttaccctaac tctcgcaata aggtggtgaa gctgagcctg     3420

aagccatacc ggttcgacgt gtacctggac aacggcgtgt ataagtttgt gacagtgaag     3480

aatctggatg tgatcaagaa ggagaactac tatgaggtga acagcaagtg ctacgaggag     3540

gccaagaagc tgaagaagat cagcaaccag gccgagttca tcgcctcttt ttacaacaat     3600

gacctgatca agatcaatgg cgagctgtat agagtgatcg gcgtgaacaa tgatctgctg     3660

aacagaatcg aagtgaatat gatcgacatc acctacaggg agtatctgga gaacatgaat     3720

gataagaggc cccctcgcat catcaagacc atcgcctcta agacacagag catcaagaag     3780

tacagcacag acatcctggg gaacctgtat gaagtcaaga gcaagaaaca tcctcagatt     3840

atcaagaaag gctctggtgg ttctaagcga cctgccgcca caaagaaggc tggacaggct     3900

aagaagaaga aagattacaa agacgatgac gataagggat ccggcgcaac aaacttctct     3960

ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgatggccaa gcctttgtct     4020

caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct     4080

gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc     4140

aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct     4200

gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc     4260

ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc     4320

atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct     4380

ggttatgtgt gggagggcta a                                               4401


<210>  132
<211>  4401
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nSaCas9 E125 miniABEMax V82G

<400>  132
atgaagcgaa attacattct ggggctggcc attggcatta catcagtggg ctatggcatc       60

attgactacg agacaaggga cgtgatcgac gccggcgtga gactgttcaa ggaggccaac      120

gtggagaaca atgagggccg gagatccaag aggggagcaa ggcgcctgaa gcggagaagg      180

cgccacagaa tccagagagt gaagaagctg ctgttcgatt acaacctgct gaccgaccac      240

tccgagctgt ctggcatcaa tccttatgag gccagagtga agggcctgtc ccagaagctg      300

tctgaggagg agtttagcgc cgccctgctg cacctggcaa agaggagagg cgtgcacaac      360

gtgaatgagg tggaggagcc gaagaagaaa cgtaaggtgg gtagcagcgg tagcccgaaa      420

aagaagcgca aagtgggcag cgacgcgctg gacgatttcg atctggacat gctgggcagc      480

gatgcgctgg atgatttcgg cggtggcagc tctgaggtgg agttttccca cgagtactgg      540

atgagacatg ccctgaccct ggccaagagg gcacgcgatg agagggaggt gcctgtggga      600

gccgtgctgg tgctgaacaa tagagtgatc ggcgagggct ggaacagagc catcggcctg      660

cacgacccaa cagcccatgc cgaaattatg gccctgagac agggcggcct ggtcatgcag      720

aactacagac tgattgacgc caccctgtac ggcacattcg agccttgcgt gatgtgcgcc      780

ggcgccatga tccactctag gatcggccgc gtggtgtttg gcgtgaggaa cgcaaaaacc      840

ggcgccgcag gctccctgat ggacgtgctg cactaccccg gcatgaatca ccgcgtcgaa      900

attaccgagg gaatcctggc agatgaatgt gccgccctgc tgtgctattt ctttcggatg      960

cctagacagg tgttcaatgc tcagaagaag gcccagagct ccaccgacgg cggtagcggc     1020

ggtagcggtg gtagcggcgg cagcggtggt agcggtggca gcggtggtga ggacaccggc     1080

aacgagctgt ccacaaagga gcagatcagc cgcaattcca aggccctgga ggagaagtat     1140

gtggccgagc tgcagctgga gcggctgaag aaggatggcg aggtgagggg ctccatcaat     1200

cgcttcaaga cctctgacta cgtgaaggag gccaagcagc tgctgaaggt gcagaaggcc     1260

taccaccagc tggatcagtc ctttatcgat acatatatcg acctgctgga gacaaggcgc     1320

acatactatg agggaccagg agagggctct cccttcggct ggaaggacat caaggagtgg     1380

tacgagatgc tgatgggcca ctgcacctat tttccagagg agctgagaag cgtgaagtac     1440

gcctataacg ccgatctgta caacgccctg aatgacctga acaacctggt catcaccagg     1500

gatgagaacg agaagctgga gtactatgag aagttccaga tcatcgagaa cgtgttcaag     1560

cagaagaaga agcctacact gaagcagatc gccaaggaga tcctggtgaa cgaggaggac     1620

atcaagggct accgcgtgac ctccacaggc aagccagagt tcaccaatct gaaggtgtat     1680

cacgatatca aggacatcac agcccggaag gagatcatcg agaacgccga gctgctggat     1740

cagatcgcca agatcctgac catctatcag agctccgagg acatccagga ggagctgacc     1800

aacctgaata gcgagctgac acaggaggag atcgagcaga tcagcaatct gaagggctac     1860

accggcacac acaacctgag cctgaaggcc atcaatctga tcctggatga gctgtggcac     1920

acaaacgaca atcagatcgc catctttaac cggctgaagc tggtgccaaa gaaggtggac     1980

ctgtcccagc agaaggagat cccaaccaca ctggtggacg atttcatcct gtctcccgtg     2040

gtgaagcgga gcttcatcca gagcatcaaa gtgatcaacg ccatcatcaa gaagtacggc     2100

ctgcccaatg atatcatcat cgagctggcc agggagaaga actccaagga cgcccagaag     2160

atgatcaatg agatgcagaa gaggaaccgc cagaccaatg agcggatcga ggagatcatc     2220

agaaccacag gcaaggagaa cgccaagtac ctgatcgaga agatcaagct gcacgatatg     2280

caggagggca agtgtctgta ttctctggag gccatccctc tggaggacct gctgaacaat     2340

ccattcaact acgaggtgga tcacatcatc ccccggagcg tgagcttcga caattctttt     2400

aacaataagg tgctggtgaa gcaggaggag aacagcaaga agggcaatag gacccctttc     2460

cagtacctgt ctagctccga ttctaagatc agctacgaga cattcaagaa gcacatcctg     2520

aatctggcca agggcaaggg ccgcatcagc aagaccaaga aggagtacct gctggaggag     2580

cgggacatca acagattctc cgtgcagaag gacttcatca accggaatct ggtggacacc     2640

agatacgcca cacgcggcct gatgaatctg ctgcggtctt atttcagagt gaacaatctg     2700

gatgtgaagg tgaagagcat caacggcggc ttcacctcct ttctgcggag aaagtggaag     2760

tttaagaagg agcgcaacaa gggctataag caccacgccg aggatgccct gatcatcgcc     2820

aatgccgact tcatctttaa ggagtggaag aagctggaca aggccaagaa agtgatggag     2880

aaccagatgt tcgaggagaa gcaggccgag agcatgcccg agatcgagac agagcaggag     2940

tacaaggaga ttttcatcac acctcaccag atcaagcaca tcaaggactt caaggactac     3000

aagtattctc acagggtgga taagaagccc aaccgcgagc tgatcaatga caccctgtat     3060

agcacacgga aggacgataa gggcaatacc ctgatcgtga acaatctgaa cggcctgtac     3120

gacaaggata atgacaagct gaagaagctg atcaacaagt ctcccgagaa gctgctgatg     3180

taccaccacg atcctcagac atatcagaag ctgaagctga tcatggagca gtacggcgac     3240

gagaagaacc cactgtataa gtactatgag gagacaggca actacctgac aaagtatagc     3300

aagaaggata atggccccgt gatcaagaag atcaagtact atggcaacaa gctgaatgcc     3360

cacctggaca tcaccgacga ttaccctaac tctcgcaata aggtggtgaa gctgagcctg     3420

aagccatacc ggttcgacgt gtacctggac aacggcgtgt ataagtttgt gacagtgaag     3480

aatctggatg tgatcaagaa ggagaactac tatgaggtga acagcaagtg ctacgaggag     3540

gccaagaagc tgaagaagat cagcaaccag gccgagttca tcgcctcttt ttacaacaat     3600

gacctgatca agatcaatgg cgagctgtat agagtgatcg gcgtgaacaa tgatctgctg     3660

aacagaatcg aagtgaatat gatcgacatc acctacaggg agtatctgga gaacatgaat     3720

gataagaggc cccctcgcat catcaagacc atcgcctcta agacacagag catcaagaag     3780

tacagcacag acatcctggg gaacctgtat gaagtcaaga gcaagaaaca tcctcagatt     3840

atcaagaaag gctctggtgg ttctaagcga cctgccgcca caaagaaggc tggacaggct     3900

aagaagaaga aagattacaa agacgatgac gataagggat ccggcgcaac aaacttctct     3960

ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgatggccaa gcctttgtct     4020

caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct     4080

gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc     4140

aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct     4200

gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc     4260

ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc     4320

atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct     4380

ggttatgtgt gggagggcta a                                               4401


<210>  133
<211>  4401
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nSaCas9 E131 miniABEMax V82G

<400>  133
atgaagcgaa attacattct ggggctggcc attggcatta catcagtggg ctatggcatc       60

attgactacg agacaaggga cgtgatcgac gccggcgtga gactgttcaa ggaggccaac      120

gtggagaaca atgagggccg gagatccaag aggggagcaa ggcgcctgaa gcggagaagg      180

cgccacagaa tccagagagt gaagaagctg ctgttcgatt acaacctgct gaccgaccac      240

tccgagctgt ctggcatcaa tccttatgag gccagagtga agggcctgtc ccagaagctg      300

tctgaggagg agtttagcgc cgccctgctg cacctggcaa agaggagagg cgtgcacaac      360

gtgaatgagg tggaggagga caccggcaac gagccgaaga agaaacgtaa ggtgggtagc      420

agcggtagcc cgaaaaagaa gcgcaaagtg ggcagcgacg cgctggacga tttcgatctg      480

gacatgctgg gcagcgatgc gctggatgat ttcggcggtg gcagctctga ggtggagttt      540

tcccacgagt actggatgag acatgccctg accctggcca agagggcacg cgatgagagg      600

gaggtgcctg tgggagccgt gctggtgctg aacaatagag tgatcggcga gggctggaac      660

agagccatcg gcctgcacga cccaacagcc catgccgaaa ttatggccct gagacagggc      720

ggcctggtca tgcagaacta cagactgatt gacgccaccc tgtacggcac attcgagcct      780

tgcgtgatgt gcgccggcgc catgatccac tctaggatcg gccgcgtggt gtttggcgtg      840

aggaacgcaa aaaccggcgc cgcaggctcc ctgatggacg tgctgcacta ccccggcatg      900

aatcaccgcg tcgaaattac cgagggaatc ctggcagatg aatgtgccgc cctgctgtgc      960

tatttctttc ggatgcctag acaggtgttc aatgctcaga agaaggccca gagctccacc     1020

gacggcggta gcggcggtag cggtggtagc ggcggcagcg gtggtagcgg tggcagcggt     1080

ggtgagctgt ccacaaagga gcagatcagc cgcaattcca aggccctgga ggagaagtat     1140

gtggccgagc tgcagctgga gcggctgaag aaggatggcg aggtgagggg ctccatcaat     1200

cgcttcaaga cctctgacta cgtgaaggag gccaagcagc tgctgaaggt gcagaaggcc     1260

taccaccagc tggatcagtc ctttatcgat acatatatcg acctgctgga gacaaggcgc     1320

acatactatg agggaccagg agagggctct cccttcggct ggaaggacat caaggagtgg     1380

tacgagatgc tgatgggcca ctgcacctat tttccagagg agctgagaag cgtgaagtac     1440

gcctataacg ccgatctgta caacgccctg aatgacctga acaacctggt catcaccagg     1500

gatgagaacg agaagctgga gtactatgag aagttccaga tcatcgagaa cgtgttcaag     1560

cagaagaaga agcctacact gaagcagatc gccaaggaga tcctggtgaa cgaggaggac     1620

atcaagggct accgcgtgac ctccacaggc aagccagagt tcaccaatct gaaggtgtat     1680

cacgatatca aggacatcac agcccggaag gagatcatcg agaacgccga gctgctggat     1740

cagatcgcca agatcctgac catctatcag agctccgagg acatccagga ggagctgacc     1800

aacctgaata gcgagctgac acaggaggag atcgagcaga tcagcaatct gaagggctac     1860

accggcacac acaacctgag cctgaaggcc atcaatctga tcctggatga gctgtggcac     1920

acaaacgaca atcagatcgc catctttaac cggctgaagc tggtgccaaa gaaggtggac     1980

ctgtcccagc agaaggagat cccaaccaca ctggtggacg atttcatcct gtctcccgtg     2040

gtgaagcgga gcttcatcca gagcatcaaa gtgatcaacg ccatcatcaa gaagtacggc     2100

ctgcccaatg atatcatcat cgagctggcc agggagaaga actccaagga cgcccagaag     2160

atgatcaatg agatgcagaa gaggaaccgc cagaccaatg agcggatcga ggagatcatc     2220

agaaccacag gcaaggagaa cgccaagtac ctgatcgaga agatcaagct gcacgatatg     2280

caggagggca agtgtctgta ttctctggag gccatccctc tggaggacct gctgaacaat     2340

ccattcaact acgaggtgga tcacatcatc ccccggagcg tgagcttcga caattctttt     2400

aacaataagg tgctggtgaa gcaggaggag aacagcaaga agggcaatag gacccctttc     2460

cagtacctgt ctagctccga ttctaagatc agctacgaga cattcaagaa gcacatcctg     2520

aatctggcca agggcaaggg ccgcatcagc aagaccaaga aggagtacct gctggaggag     2580

cgggacatca acagattctc cgtgcagaag gacttcatca accggaatct ggtggacacc     2640

agatacgcca cacgcggcct gatgaatctg ctgcggtctt atttcagagt gaacaatctg     2700

gatgtgaagg tgaagagcat caacggcggc ttcacctcct ttctgcggag aaagtggaag     2760

tttaagaagg agcgcaacaa gggctataag caccacgccg aggatgccct gatcatcgcc     2820

aatgccgact tcatctttaa ggagtggaag aagctggaca aggccaagaa agtgatggag     2880

aaccagatgt tcgaggagaa gcaggccgag agcatgcccg agatcgagac agagcaggag     2940

tacaaggaga ttttcatcac acctcaccag atcaagcaca tcaaggactt caaggactac     3000

aagtattctc acagggtgga taagaagccc aaccgcgagc tgatcaatga caccctgtat     3060

agcacacgga aggacgataa gggcaatacc ctgatcgtga acaatctgaa cggcctgtac     3120

gacaaggata atgacaagct gaagaagctg atcaacaagt ctcccgagaa gctgctgatg     3180

taccaccacg atcctcagac atatcagaag ctgaagctga tcatggagca gtacggcgac     3240

gagaagaacc cactgtataa gtactatgag gagacaggca actacctgac aaagtatagc     3300

aagaaggata atggccccgt gatcaagaag atcaagtact atggcaacaa gctgaatgcc     3360

cacctggaca tcaccgacga ttaccctaac tctcgcaata aggtggtgaa gctgagcctg     3420

aagccatacc ggttcgacgt gtacctggac aacggcgtgt ataagtttgt gacagtgaag     3480

aatctggatg tgatcaagaa ggagaactac tatgaggtga acagcaagtg ctacgaggag     3540

gccaagaagc tgaagaagat cagcaaccag gccgagttca tcgcctcttt ttacaacaat     3600

gacctgatca agatcaatgg cgagctgtat agagtgatcg gcgtgaacaa tgatctgctg     3660

aacagaatcg aagtgaatat gatcgacatc acctacaggg agtatctgga gaacatgaat     3720

gataagaggc cccctcgcat catcaagacc atcgcctcta agacacagag catcaagaag     3780

tacagcacag acatcctggg gaacctgtat gaagtcaaga gcaagaaaca tcctcagatt     3840

atcaagaaag gctctggtgg ttctaagcga cctgccgcca caaagaaggc tggacaggct     3900

aagaagaaga aagattacaa agacgatgac gataagggat ccggcgcaac aaacttctct     3960

ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgatggccaa gcctttgtct     4020

caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct     4080

gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc     4140

aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct     4200

gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc     4260

ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc     4320

atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct     4380

ggttatgtgt gggagggcta a                                               4401


<210>  134
<211>  4401
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nSaCas9 L132 miniABEMax V82G

<400>  134
atgaagcgaa attacattct ggggctggcc attggcatta catcagtggg ctatggcatc       60

attgactacg agacaaggga cgtgatcgac gccggcgtga gactgttcaa ggaggccaac      120

gtggagaaca atgagggccg gagatccaag aggggagcaa ggcgcctgaa gcggagaagg      180

cgccacagaa tccagagagt gaagaagctg ctgttcgatt acaacctgct gaccgaccac      240

tccgagctgt ctggcatcaa tccttatgag gccagagtga agggcctgtc ccagaagctg      300

tctgaggagg agtttagcgc cgccctgctg cacctggcaa agaggagagg cgtgcacaac      360

gtgaatgagg tggaggagga caccggcaac gagctgccga agaagaaacg taaggtgggt      420

agcagcggta gcccgaaaaa gaagcgcaaa gtgggcagcg acgcgctgga cgatttcgat      480

ctggacatgc tgggcagcga tgcgctggat gatttcggcg gtggcagctc tgaggtggag      540

ttttcccacg agtactggat gagacatgcc ctgaccctgg ccaagagggc acgcgatgag      600

agggaggtgc ctgtgggagc cgtgctggtg ctgaacaata gagtgatcgg cgagggctgg      660

aacagagcca tcggcctgca cgacccaaca gcccatgccg aaattatggc cctgagacag      720

ggcggcctgg tcatgcagaa ctacagactg attgacgcca ccctgtacgg cacattcgag      780

ccttgcgtga tgtgcgccgg cgccatgatc cactctagga tcggccgcgt ggtgtttggc      840

gtgaggaacg caaaaaccgg cgccgcaggc tccctgatgg acgtgctgca ctaccccggc      900

atgaatcacc gcgtcgaaat taccgaggga atcctggcag atgaatgtgc cgccctgctg      960

tgctatttct ttcggatgcc tagacaggtg ttcaatgctc agaagaaggc ccagagctcc     1020

accgacggcg gtagcggcgg tagcggtggt agcggcggca gcggtggtag cggtggcagc     1080

ggtggtctgt ccacaaagga gcagatcagc cgcaattcca aggccctgga ggagaagtat     1140

gtggccgagc tgcagctgga gcggctgaag aaggatggcg aggtgagggg ctccatcaat     1200

cgcttcaaga cctctgacta cgtgaaggag gccaagcagc tgctgaaggt gcagaaggcc     1260

taccaccagc tggatcagtc ctttatcgat acatatatcg acctgctgga gacaaggcgc     1320

acatactatg agggaccagg agagggctct cccttcggct ggaaggacat caaggagtgg     1380

tacgagatgc tgatgggcca ctgcacctat tttccagagg agctgagaag cgtgaagtac     1440

gcctataacg ccgatctgta caacgccctg aatgacctga acaacctggt catcaccagg     1500

gatgagaacg agaagctgga gtactatgag aagttccaga tcatcgagaa cgtgttcaag     1560

cagaagaaga agcctacact gaagcagatc gccaaggaga tcctggtgaa cgaggaggac     1620

atcaagggct accgcgtgac ctccacaggc aagccagagt tcaccaatct gaaggtgtat     1680

cacgatatca aggacatcac agcccggaag gagatcatcg agaacgccga gctgctggat     1740

cagatcgcca agatcctgac catctatcag agctccgagg acatccagga ggagctgacc     1800

aacctgaata gcgagctgac acaggaggag atcgagcaga tcagcaatct gaagggctac     1860

accggcacac acaacctgag cctgaaggcc atcaatctga tcctggatga gctgtggcac     1920

acaaacgaca atcagatcgc catctttaac cggctgaagc tggtgccaaa gaaggtggac     1980

ctgtcccagc agaaggagat cccaaccaca ctggtggacg atttcatcct gtctcccgtg     2040

gtgaagcgga gcttcatcca gagcatcaaa gtgatcaacg ccatcatcaa gaagtacggc     2100

ctgcccaatg atatcatcat cgagctggcc agggagaaga actccaagga cgcccagaag     2160

atgatcaatg agatgcagaa gaggaaccgc cagaccaatg agcggatcga ggagatcatc     2220

agaaccacag gcaaggagaa cgccaagtac ctgatcgaga agatcaagct gcacgatatg     2280

caggagggca agtgtctgta ttctctggag gccatccctc tggaggacct gctgaacaat     2340

ccattcaact acgaggtgga tcacatcatc ccccggagcg tgagcttcga caattctttt     2400

aacaataagg tgctggtgaa gcaggaggag aacagcaaga agggcaatag gacccctttc     2460

cagtacctgt ctagctccga ttctaagatc agctacgaga cattcaagaa gcacatcctg     2520

aatctggcca agggcaaggg ccgcatcagc aagaccaaga aggagtacct gctggaggag     2580

cgggacatca acagattctc cgtgcagaag gacttcatca accggaatct ggtggacacc     2640

agatacgcca cacgcggcct gatgaatctg ctgcggtctt atttcagagt gaacaatctg     2700

gatgtgaagg tgaagagcat caacggcggc ttcacctcct ttctgcggag aaagtggaag     2760

tttaagaagg agcgcaacaa gggctataag caccacgccg aggatgccct gatcatcgcc     2820

aatgccgact tcatctttaa ggagtggaag aagctggaca aggccaagaa agtgatggag     2880

aaccagatgt tcgaggagaa gcaggccgag agcatgcccg agatcgagac agagcaggag     2940

tacaaggaga ttttcatcac acctcaccag atcaagcaca tcaaggactt caaggactac     3000

aagtattctc acagggtgga taagaagccc aaccgcgagc tgatcaatga caccctgtat     3060

agcacacgga aggacgataa gggcaatacc ctgatcgtga acaatctgaa cggcctgtac     3120

gacaaggata atgacaagct gaagaagctg atcaacaagt ctcccgagaa gctgctgatg     3180

taccaccacg atcctcagac atatcagaag ctgaagctga tcatggagca gtacggcgac     3240

gagaagaacc cactgtataa gtactatgag gagacaggca actacctgac aaagtatagc     3300

aagaaggata atggccccgt gatcaagaag atcaagtact atggcaacaa gctgaatgcc     3360

cacctggaca tcaccgacga ttaccctaac tctcgcaata aggtggtgaa gctgagcctg     3420

aagccatacc ggttcgacgt gtacctggac aacggcgtgt ataagtttgt gacagtgaag     3480

aatctggatg tgatcaagaa ggagaactac tatgaggtga acagcaagtg ctacgaggag     3540

gccaagaagc tgaagaagat cagcaaccag gccgagttca tcgcctcttt ttacaacaat     3600

gacctgatca agatcaatgg cgagctgtat agagtgatcg gcgtgaacaa tgatctgctg     3660

aacagaatcg aagtgaatat gatcgacatc acctacaggg agtatctgga gaacatgaat     3720

gataagaggc cccctcgcat catcaagacc atcgcctcta agacacagag catcaagaag     3780

tacagcacag acatcctggg gaacctgtat gaagtcaaga gcaagaaaca tcctcagatt     3840

atcaagaaag gctctggtgg ttctaagcga cctgccgcca caaagaaggc tggacaggct     3900

aagaagaaga aagattacaa agacgatgac gataagggat ccggcgcaac aaacttctct     3960

ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgatggccaa gcctttgtct     4020

caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct     4080

gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc     4140

aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct     4200

gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc     4260

ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc     4320

atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct     4380

ggttatgtgt gggagggcta a                                               4401


<210>  135
<211>  4401
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nSaCas9 N130 miniABEMax V82G

<400>  135
atgaagcgaa attacattct ggggctggcc attggcatta catcagtggg ctatggcatc       60

attgactacg agacaaggga cgtgatcgac gccggcgtga gactgttcaa ggaggccaac      120

gtggagaaca atgagggccg gagatccaag aggggagcaa ggcgcctgaa gcggagaagg      180

cgccacagaa tccagagagt gaagaagctg ctgttcgatt acaacctgct gaccgaccac      240

tccgagctgt ctggcatcaa tccttatgag gccagagtga agggcctgtc ccagaagctg      300

tctgaggagg agtttagcgc cgccctgctg cacctggcaa agaggagagg cgtgcacaac      360

gtgaatgagg tggaggagga caccggcaac ccgaagaaga aacgtaaggt gggtagcagc      420

ggtagcccga aaaagaagcg caaagtgggc agcgacgcgc tggacgattt cgatctggac      480

atgctgggca gcgatgcgct ggatgatttc ggcggtggca gctctgaggt ggagttttcc      540

cacgagtact ggatgagaca tgccctgacc ctggccaaga gggcacgcga tgagagggag      600

gtgcctgtgg gagccgtgct ggtgctgaac aatagagtga tcggcgaggg ctggaacaga      660

gccatcggcc tgcacgaccc aacagcccat gccgaaatta tggccctgag acagggcggc      720

ctggtcatgc agaactacag actgattgac gccaccctgt acggcacatt cgagccttgc      780

gtgatgtgcg ccggcgccat gatccactct aggatcggcc gcgtggtgtt tggcgtgagg      840

aacgcaaaaa ccggcgccgc aggctccctg atggacgtgc tgcactaccc cggcatgaat      900

caccgcgtcg aaattaccga gggaatcctg gcagatgaat gtgccgccct gctgtgctat      960

ttctttcgga tgcctagaca ggtgttcaat gctcagaaga aggcccagag ctccaccgac     1020

ggcggtagcg gcggtagcgg tggtagcggc ggcagcggtg gtagcggtgg cagcggtggt     1080

aacgagctgt ccacaaagga gcagatcagc cgcaattcca aggccctgga ggagaagtat     1140

gtggccgagc tgcagctgga gcggctgaag aaggatggcg aggtgagggg ctccatcaat     1200

cgcttcaaga cctctgacta cgtgaaggag gccaagcagc tgctgaaggt gcagaaggcc     1260

taccaccagc tggatcagtc ctttatcgat acatatatcg acctgctgga gacaaggcgc     1320

acatactatg agggaccagg agagggctct cccttcggct ggaaggacat caaggagtgg     1380

tacgagatgc tgatgggcca ctgcacctat tttccagagg agctgagaag cgtgaagtac     1440

gcctataacg ccgatctgta caacgccctg aatgacctga acaacctggt catcaccagg     1500

gatgagaacg agaagctgga gtactatgag aagttccaga tcatcgagaa cgtgttcaag     1560

cagaagaaga agcctacact gaagcagatc gccaaggaga tcctggtgaa cgaggaggac     1620

atcaagggct accgcgtgac ctccacaggc aagccagagt tcaccaatct gaaggtgtat     1680

cacgatatca aggacatcac agcccggaag gagatcatcg agaacgccga gctgctggat     1740

cagatcgcca agatcctgac catctatcag agctccgagg acatccagga ggagctgacc     1800

aacctgaata gcgagctgac acaggaggag atcgagcaga tcagcaatct gaagggctac     1860

accggcacac acaacctgag cctgaaggcc atcaatctga tcctggatga gctgtggcac     1920

acaaacgaca atcagatcgc catctttaac cggctgaagc tggtgccaaa gaaggtggac     1980

ctgtcccagc agaaggagat cccaaccaca ctggtggacg atttcatcct gtctcccgtg     2040

gtgaagcgga gcttcatcca gagcatcaaa gtgatcaacg ccatcatcaa gaagtacggc     2100

ctgcccaatg atatcatcat cgagctggcc agggagaaga actccaagga cgcccagaag     2160

atgatcaatg agatgcagaa gaggaaccgc cagaccaatg agcggatcga ggagatcatc     2220

agaaccacag gcaaggagaa cgccaagtac ctgatcgaga agatcaagct gcacgatatg     2280

caggagggca agtgtctgta ttctctggag gccatccctc tggaggacct gctgaacaat     2340

ccattcaact acgaggtgga tcacatcatc ccccggagcg tgagcttcga caattctttt     2400

aacaataagg tgctggtgaa gcaggaggag aacagcaaga agggcaatag gacccctttc     2460

cagtacctgt ctagctccga ttctaagatc agctacgaga cattcaagaa gcacatcctg     2520

aatctggcca agggcaaggg ccgcatcagc aagaccaaga aggagtacct gctggaggag     2580

cgggacatca acagattctc cgtgcagaag gacttcatca accggaatct ggtggacacc     2640

agatacgcca cacgcggcct gatgaatctg ctgcggtctt atttcagagt gaacaatctg     2700

gatgtgaagg tgaagagcat caacggcggc ttcacctcct ttctgcggag aaagtggaag     2760

tttaagaagg agcgcaacaa gggctataag caccacgccg aggatgccct gatcatcgcc     2820

aatgccgact tcatctttaa ggagtggaag aagctggaca aggccaagaa agtgatggag     2880

aaccagatgt tcgaggagaa gcaggccgag agcatgcccg agatcgagac agagcaggag     2940

tacaaggaga ttttcatcac acctcaccag atcaagcaca tcaaggactt caaggactac     3000

aagtattctc acagggtgga taagaagccc aaccgcgagc tgatcaatga caccctgtat     3060

agcacacgga aggacgataa gggcaatacc ctgatcgtga acaatctgaa cggcctgtac     3120

gacaaggata atgacaagct gaagaagctg atcaacaagt ctcccgagaa gctgctgatg     3180

taccaccacg atcctcagac atatcagaag ctgaagctga tcatggagca gtacggcgac     3240

gagaagaacc cactgtataa gtactatgag gagacaggca actacctgac aaagtatagc     3300

aagaaggata atggccccgt gatcaagaag atcaagtact atggcaacaa gctgaatgcc     3360

cacctggaca tcaccgacga ttaccctaac tctcgcaata aggtggtgaa gctgagcctg     3420

aagccatacc ggttcgacgt gtacctggac aacggcgtgt ataagtttgt gacagtgaag     3480

aatctggatg tgatcaagaa ggagaactac tatgaggtga acagcaagtg ctacgaggag     3540

gccaagaagc tgaagaagat cagcaaccag gccgagttca tcgcctcttt ttacaacaat     3600

gacctgatca agatcaatgg cgagctgtat agagtgatcg gcgtgaacaa tgatctgctg     3660

aacagaatcg aagtgaatat gatcgacatc acctacaggg agtatctgga gaacatgaat     3720

gataagaggc cccctcgcat catcaagacc atcgcctcta agacacagag catcaagaag     3780

tacagcacag acatcctggg gaacctgtat gaagtcaaga gcaagaaaca tcctcagatt     3840

atcaagaaag gctctggtgg ttctaagcga cctgccgcca caaagaaggc tggacaggct     3900

aagaagaaga aagattacaa agacgatgac gataagggat ccggcgcaac aaacttctct     3960

ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgatggccaa gcctttgtct     4020

caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct     4080

gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc     4140

aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct     4200

gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc     4260

ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc     4320

atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct     4380

ggttatgtgt gggagggcta a                                               4401


<210>  136
<211>  4401
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nSaCas9 E735 miniABEMax V82G

<400>  136
atgaagcgaa attacattct ggggctggcc attggcatta catcagtggg ctatggcatc       60

attgactacg agacaaggga cgtgatcgac gccggcgtga gactgttcaa ggaggccaac      120

gtggagaaca atgagggccg gagatccaag aggggagcaa ggcgcctgaa gcggagaagg      180

cgccacagaa tccagagagt gaagaagctg ctgttcgatt acaacctgct gaccgaccac      240

tccgagctgt ctggcatcaa tccttatgag gccagagtga agggcctgtc ccagaagctg      300

tctgaggagg agtttagcgc cgccctgctg cacctggcaa agaggagagg cgtgcacaac      360

gtgaatgagg tggaggagga caccggcaac gagctgtcca caaaggagca gatcagccgc      420

aattccaagg ccctggagga gaagtatgtg gccgagctgc agctggagcg gctgaagaag      480

gatggcgagg tgaggggctc catcaatcgc ttcaagacct ctgactacgt gaaggaggcc      540

aagcagctgc tgaaggtgca gaaggcctac caccagctgg atcagtcctt tatcgataca      600

tatatcgacc tgctggagac aaggcgcaca tactatgagg gaccaggaga gggctctccc      660

ttcggctgga aggacatcaa ggagtggtac gagatgctga tgggccactg cacctatttt      720

ccagaggagc tgagaagcgt gaagtacgcc tataacgccg atctgtacaa cgccctgaat      780

gacctgaaca acctggtcat caccagggat gagaacgaga agctggagta ctatgagaag      840

ttccagatca tcgagaacgt gttcaagcag aagaagaagc ctacactgaa gcagatcgcc      900

aaggagatcc tggtgaacga ggaggacatc aagggctacc gcgtgacctc cacaggcaag      960

ccagagttca ccaatctgaa ggtgtatcac gatatcaagg acatcacagc ccggaaggag     1020

atcatcgaga acgccgagct gctggatcag atcgccaaga tcctgaccat ctatcagagc     1080

tccgaggaca tccaggagga gctgaccaac ctgaatagcg agctgacaca ggaggagatc     1140

gagcagatca gcaatctgaa gggctacacc ggcacacaca acctgagcct gaaggccatc     1200

aatctgatcc tggatgagct gtggcacaca aacgacaatc agatcgccat ctttaaccgg     1260

ctgaagctgg tgccaaagaa ggtggacctg tcccagcaga aggagatccc aaccacactg     1320

gtggacgatt tcatcctgtc tcccgtggtg aagcggagct tcatccagag catcaaagtg     1380

atcaacgcca tcatcaagaa gtacggcctg cccaatgata tcatcatcga gctggccagg     1440

gagaagaact ccaaggacgc ccagaagatg atcaatgaga tgcagaagag gaaccgccag     1500

accaatgagc ggatcgagga gatcatcaga accacaggca aggagaacgc caagtacctg     1560

atcgagaaga tcaagctgca cgatatgcag gagggcaagt gtctgtattc tctggaggcc     1620

atccctctgg aggacctgct gaacaatcca ttcaactacg aggtggatca catcatcccc     1680

cggagcgtga gcttcgacaa ttcttttaac aataaggtgc tggtgaagca ggaggagaac     1740

agcaagaagg gcaataggac ccctttccag tacctgtcta gctccgattc taagatcagc     1800

tacgagacat tcaagaagca catcctgaat ctggccaagg gcaagggccg catcagcaag     1860

accaagaagg agtacctgct ggaggagcgg gacatcaaca gattctccgt gcagaaggac     1920

ttcatcaacc ggaatctggt ggacaccaga tacgccacac gcggcctgat gaatctgctg     1980

cggtcttatt tcagagtgaa caatctggat gtgaaggtga agagcatcaa cggcggcttc     2040

acctcctttc tgcggagaaa gtggaagttt aagaaggagc gcaacaaggg ctataagcac     2100

cacgccgagg atgccctgat catcgccaat gccgacttca tctttaagga gtggaagaag     2160

ctggacaagg ccaagaaagt gatggagaac cagatgttcg aggagccgaa gaagaaacgt     2220

aaggtgggta gcagcggtag cccgaaaaag aagcgcaaag tgggcagcga cgcgctggac     2280

gatttcgatc tggacatgct gggcagcgat gcgctggatg atttcggcgg tggcagctct     2340

gaggtggagt tttcccacga gtactggatg agacatgccc tgaccctggc caagagggca     2400

cgcgatgaga gggaggtgcc tgtgggagcc gtgctggtgc tgaacaatag agtgatcggc     2460

gagggctgga acagagccat cggcctgcac gacccaacag cccatgccga aattatggcc     2520

ctgagacagg gcggcctggt catgcagaac tacagactga ttgacgccac cctgtacggc     2580

acattcgagc cttgcgtgat gtgcgccggc gccatgatcc actctaggat cggccgcgtg     2640

gtgtttggcg tgaggaacgc aaaaaccggc gccgcaggct ccctgatgga cgtgctgcac     2700

taccccggca tgaatcaccg cgtcgaaatt accgagggaa tcctggcaga tgaatgtgcc     2760

gccctgctgt gctatttctt tcggatgcct agacaggtgt tcaatgctca gaagaaggcc     2820

cagagctcca ccgacggcgg tagcggcggt agcggtggta gcggcggcag cggtggtagc     2880

ggtggcagcg gtggtgagaa gcaggccgag agcatgcccg agatcgagac agagcaggag     2940

tacaaggaga ttttcatcac acctcaccag atcaagcaca tcaaggactt caaggactac     3000

aagtattctc acagggtgga taagaagccc aaccgcgagc tgatcaatga caccctgtat     3060

agcacacgga aggacgataa gggcaatacc ctgatcgtga acaatctgaa cggcctgtac     3120

gacaaggata atgacaagct gaagaagctg atcaacaagt ctcccgagaa gctgctgatg     3180

taccaccacg atcctcagac atatcagaag ctgaagctga tcatggagca gtacggcgac     3240

gagaagaacc cactgtataa gtactatgag gagacaggca actacctgac aaagtatagc     3300

aagaaggata atggccccgt gatcaagaag atcaagtact atggcaacaa gctgaatgcc     3360

cacctggaca tcaccgacga ttaccctaac tctcgcaata aggtggtgaa gctgagcctg     3420

aagccatacc ggttcgacgt gtacctggac aacggcgtgt ataagtttgt gacagtgaag     3480

aatctggatg tgatcaagaa ggagaactac tatgaggtga acagcaagtg ctacgaggag     3540

gccaagaagc tgaagaagat cagcaaccag gccgagttca tcgcctcttt ttacaacaat     3600

gacctgatca agatcaatgg cgagctgtat agagtgatcg gcgtgaacaa tgatctgctg     3660

aacagaatcg aagtgaatat gatcgacatc acctacaggg agtatctgga gaacatgaat     3720

gataagaggc cccctcgcat catcaagacc atcgcctcta agacacagag catcaagaag     3780

tacagcacag acatcctggg gaacctgtat gaagtcaaga gcaagaaaca tcctcagatt     3840

atcaagaaag gctctggtgg ttctaagcga cctgccgcca caaagaaggc tggacaggct     3900

aagaagaaga aagattacaa agacgatgac gataagggat ccggcgcaac aaacttctct     3960

ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgatggccaa gcctttgtct     4020

caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct     4080

gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc     4140

aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct     4200

gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc     4260

ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc     4320

atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct     4380

ggttatgtgt gggagggcta a                                               4401


<210>  137
<211>  4401
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nSaCas9 K736 miniABEMax V82G

<400>  137
atgaagcgaa attacattct ggggctggcc attggcatta catcagtggg ctatggcatc       60

attgactacg agacaaggga cgtgatcgac gccggcgtga gactgttcaa ggaggccaac      120

gtggagaaca atgagggccg gagatccaag aggggagcaa ggcgcctgaa gcggagaagg      180

cgccacagaa tccagagagt gaagaagctg ctgttcgatt acaacctgct gaccgaccac      240

tccgagctgt ctggcatcaa tccttatgag gccagagtga agggcctgtc ccagaagctg      300

tctgaggagg agtttagcgc cgccctgctg cacctggcaa agaggagagg cgtgcacaac      360

gtgaatgagg tggaggagga caccggcaac gagctgtcca caaaggagca gatcagccgc      420

aattccaagg ccctggagga gaagtatgtg gccgagctgc agctggagcg gctgaagaag      480

gatggcgagg tgaggggctc catcaatcgc ttcaagacct ctgactacgt gaaggaggcc      540

aagcagctgc tgaaggtgca gaaggcctac caccagctgg atcagtcctt tatcgataca      600

tatatcgacc tgctggagac aaggcgcaca tactatgagg gaccaggaga gggctctccc      660

ttcggctgga aggacatcaa ggagtggtac gagatgctga tgggccactg cacctatttt      720

ccagaggagc tgagaagcgt gaagtacgcc tataacgccg atctgtacaa cgccctgaat      780

gacctgaaca acctggtcat caccagggat gagaacgaga agctggagta ctatgagaag      840

ttccagatca tcgagaacgt gttcaagcag aagaagaagc ctacactgaa gcagatcgcc      900

aaggagatcc tggtgaacga ggaggacatc aagggctacc gcgtgacctc cacaggcaag      960

ccagagttca ccaatctgaa ggtgtatcac gatatcaagg acatcacagc ccggaaggag     1020

atcatcgaga acgccgagct gctggatcag atcgccaaga tcctgaccat ctatcagagc     1080

tccgaggaca tccaggagga gctgaccaac ctgaatagcg agctgacaca ggaggagatc     1140

gagcagatca gcaatctgaa gggctacacc ggcacacaca acctgagcct gaaggccatc     1200

aatctgatcc tggatgagct gtggcacaca aacgacaatc agatcgccat ctttaaccgg     1260

ctgaagctgg tgccaaagaa ggtggacctg tcccagcaga aggagatccc aaccacactg     1320

gtggacgatt tcatcctgtc tcccgtggtg aagcggagct tcatccagag catcaaagtg     1380

atcaacgcca tcatcaagaa gtacggcctg cccaatgata tcatcatcga gctggccagg     1440

gagaagaact ccaaggacgc ccagaagatg atcaatgaga tgcagaagag gaaccgccag     1500

accaatgagc ggatcgagga gatcatcaga accacaggca aggagaacgc caagtacctg     1560

atcgagaaga tcaagctgca cgatatgcag gagggcaagt gtctgtattc tctggaggcc     1620

atccctctgg aggacctgct gaacaatcca ttcaactacg aggtggatca catcatcccc     1680

cggagcgtga gcttcgacaa ttcttttaac aataaggtgc tggtgaagca ggaggagaac     1740

agcaagaagg gcaataggac ccctttccag tacctgtcta gctccgattc taagatcagc     1800

tacgagacat tcaagaagca catcctgaat ctggccaagg gcaagggccg catcagcaag     1860

accaagaagg agtacctgct ggaggagcgg gacatcaaca gattctccgt gcagaaggac     1920

ttcatcaacc ggaatctggt ggacaccaga tacgccacac gcggcctgat gaatctgctg     1980

cggtcttatt tcagagtgaa caatctggat gtgaaggtga agagcatcaa cggcggcttc     2040

acctcctttc tgcggagaaa gtggaagttt aagaaggagc gcaacaaggg ctataagcac     2100

cacgccgagg atgccctgat catcgccaat gccgacttca tctttaagga gtggaagaag     2160

ctggacaagg ccaagaaagt gatggagaac cagatgttcg aggagaagcc gaagaagaaa     2220

cgtaaggtgg gtagcagcgg tagcccgaaa aagaagcgca aagtgggcag cgacgcgctg     2280

gacgatttcg atctggacat gctgggcagc gatgcgctgg atgatttcgg cggtggcagc     2340

tctgaggtgg agttttccca cgagtactgg atgagacatg ccctgaccct ggccaagagg     2400

gcacgcgatg agagggaggt gcctgtggga gccgtgctgg tgctgaacaa tagagtgatc     2460

ggcgagggct ggaacagagc catcggcctg cacgacccaa cagcccatgc cgaaattatg     2520

gccctgagac agggcggcct ggtcatgcag aactacagac tgattgacgc caccctgtac     2580

ggcacattcg agccttgcgt gatgtgcgcc ggcgccatga tccactctag gatcggccgc     2640

gtggtgtttg gcgtgaggaa cgcaaaaacc ggcgccgcag gctccctgat ggacgtgctg     2700

cactaccccg gcatgaatca ccgcgtcgaa attaccgagg gaatcctggc agatgaatgt     2760

gccgccctgc tgtgctattt ctttcggatg cctagacagg tgttcaatgc tcagaagaag     2820

gcccagagct ccaccgacgg cggtagcggc ggtagcggtg gtagcggcgg cagcggtggt     2880

agcggtggca gcggtggtaa gcaggccgag agcatgcccg agatcgagac agagcaggag     2940

tacaaggaga ttttcatcac acctcaccag atcaagcaca tcaaggactt caaggactac     3000

aagtattctc acagggtgga taagaagccc aaccgcgagc tgatcaatga caccctgtat     3060

agcacacgga aggacgataa gggcaatacc ctgatcgtga acaatctgaa cggcctgtac     3120

gacaaggata atgacaagct gaagaagctg atcaacaagt ctcccgagaa gctgctgatg     3180

taccaccacg atcctcagac atatcagaag ctgaagctga tcatggagca gtacggcgac     3240

gagaagaacc cactgtataa gtactatgag gagacaggca actacctgac aaagtatagc     3300

aagaaggata atggccccgt gatcaagaag atcaagtact atggcaacaa gctgaatgcc     3360

cacctggaca tcaccgacga ttaccctaac tctcgcaata aggtggtgaa gctgagcctg     3420

aagccatacc ggttcgacgt gtacctggac aacggcgtgt ataagtttgt gacagtgaag     3480

aatctggatg tgatcaagaa ggagaactac tatgaggtga acagcaagtg ctacgaggag     3540

gccaagaagc tgaagaagat cagcaaccag gccgagttca tcgcctcttt ttacaacaat     3600

gacctgatca agatcaatgg cgagctgtat agagtgatcg gcgtgaacaa tgatctgctg     3660

aacagaatcg aagtgaatat gatcgacatc acctacaggg agtatctgga gaacatgaat     3720

gataagaggc cccctcgcat catcaagacc atcgcctcta agacacagag catcaagaag     3780

tacagcacag acatcctggg gaacctgtat gaagtcaaga gcaagaaaca tcctcagatt     3840

atcaagaaag gctctggtgg ttctaagcga cctgccgcca caaagaaggc tggacaggct     3900

aagaagaaga aagattacaa agacgatgac gataagggat ccggcgcaac aaacttctct     3960

ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgatggccaa gcctttgtct     4020

caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct     4080

gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc     4140

aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct     4200

gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc     4260

ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc     4320

atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct     4380

ggttatgtgt gggagggcta a                                               4401


<210>  138
<211>  4401
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nSaCas9 I744 miniABEMax V82G

<400>  138
atgaagcgaa attacattct ggggctggcc attggcatta catcagtggg ctatggcatc       60

attgactacg agacaaggga cgtgatcgac gccggcgtga gactgttcaa ggaggccaac      120

gtggagaaca atgagggccg gagatccaag aggggagcaa ggcgcctgaa gcggagaagg      180

cgccacagaa tccagagagt gaagaagctg ctgttcgatt acaacctgct gaccgaccac      240

tccgagctgt ctggcatcaa tccttatgag gccagagtga agggcctgtc ccagaagctg      300

tctgaggagg agtttagcgc cgccctgctg cacctggcaa agaggagagg cgtgcacaac      360

gtgaatgagg tggaggagga caccggcaac gagctgtcca caaaggagca gatcagccgc      420

aattccaagg ccctggagga gaagtatgtg gccgagctgc agctggagcg gctgaagaag      480

gatggcgagg tgaggggctc catcaatcgc ttcaagacct ctgactacgt gaaggaggcc      540

aagcagctgc tgaaggtgca gaaggcctac caccagctgg atcagtcctt tatcgataca      600

tatatcgacc tgctggagac aaggcgcaca tactatgagg gaccaggaga gggctctccc      660

ttcggctgga aggacatcaa ggagtggtac gagatgctga tgggccactg cacctatttt      720

ccagaggagc tgagaagcgt gaagtacgcc tataacgccg atctgtacaa cgccctgaat      780

gacctgaaca acctggtcat caccagggat gagaacgaga agctggagta ctatgagaag      840

ttccagatca tcgagaacgt gttcaagcag aagaagaagc ctacactgaa gcagatcgcc      900

aaggagatcc tggtgaacga ggaggacatc aagggctacc gcgtgacctc cacaggcaag      960

ccagagttca ccaatctgaa ggtgtatcac gatatcaagg acatcacagc ccggaaggag     1020

atcatcgaga acgccgagct gctggatcag atcgccaaga tcctgaccat ctatcagagc     1080

tccgaggaca tccaggagga gctgaccaac ctgaatagcg agctgacaca ggaggagatc     1140

gagcagatca gcaatctgaa gggctacacc ggcacacaca acctgagcct gaaggccatc     1200

aatctgatcc tggatgagct gtggcacaca aacgacaatc agatcgccat ctttaaccgg     1260

ctgaagctgg tgccaaagaa ggtggacctg tcccagcaga aggagatccc aaccacactg     1320

gtggacgatt tcatcctgtc tcccgtggtg aagcggagct tcatccagag catcaaagtg     1380

atcaacgcca tcatcaagaa gtacggcctg cccaatgata tcatcatcga gctggccagg     1440

gagaagaact ccaaggacgc ccagaagatg atcaatgaga tgcagaagag gaaccgccag     1500

accaatgagc ggatcgagga gatcatcaga accacaggca aggagaacgc caagtacctg     1560

atcgagaaga tcaagctgca cgatatgcag gagggcaagt gtctgtattc tctggaggcc     1620

atccctctgg aggacctgct gaacaatcca ttcaactacg aggtggatca catcatcccc     1680

cggagcgtga gcttcgacaa ttcttttaac aataaggtgc tggtgaagca ggaggagaac     1740

agcaagaagg gcaataggac ccctttccag tacctgtcta gctccgattc taagatcagc     1800

tacgagacat tcaagaagca catcctgaat ctggccaagg gcaagggccg catcagcaag     1860

accaagaagg agtacctgct ggaggagcgg gacatcaaca gattctccgt gcagaaggac     1920

ttcatcaacc ggaatctggt ggacaccaga tacgccacac gcggcctgat gaatctgctg     1980

cggtcttatt tcagagtgaa caatctggat gtgaaggtga agagcatcaa cggcggcttc     2040

acctcctttc tgcggagaaa gtggaagttt aagaaggagc gcaacaaggg ctataagcac     2100

cacgccgagg atgccctgat catcgccaat gccgacttca tctttaagga gtggaagaag     2160

ctggacaagg ccaagaaagt gatggagaac cagatgttcg aggagaagca ggccgagagc     2220

atgcccgaga tcccgaagaa gaaacgtaag gtgggtagca gcggtagccc gaaaaagaag     2280

cgcaaagtgg gcagcgacgc gctggacgat ttcgatctgg acatgctggg cagcgatgcg     2340

ctggatgatt tcggcggtgg cagctctgag gtggagtttt cccacgagta ctggatgaga     2400

catgccctga ccctggccaa gagggcacgc gatgagaggg aggtgcctgt gggagccgtg     2460

ctggtgctga acaatagagt gatcggcgag ggctggaaca gagccatcgg cctgcacgac     2520

ccaacagccc atgccgaaat tatggccctg agacagggcg gcctggtcat gcagaactac     2580

agactgattg acgccaccct gtacggcaca ttcgagcctt gcgtgatgtg cgccggcgcc     2640

atgatccact ctaggatcgg ccgcgtggtg tttggcgtga ggaacgcaaa aaccggcgcc     2700

gcaggctccc tgatggacgt gctgcactac cccggcatga atcaccgcgt cgaaattacc     2760

gagggaatcc tggcagatga atgtgccgcc ctgctgtgct atttctttcg gatgcctaga     2820

caggtgttca atgctcagaa gaaggcccag agctccaccg acggcggtag cggcggtagc     2880

ggtggtagcg gcggcagcgg tggtagcggt ggcagcggtg gtatcgagac agagcaggag     2940

tacaaggaga ttttcatcac acctcaccag atcaagcaca tcaaggactt caaggactac     3000

aagtattctc acagggtgga taagaagccc aaccgcgagc tgatcaatga caccctgtat     3060

agcacacgga aggacgataa gggcaatacc ctgatcgtga acaatctgaa cggcctgtac     3120

gacaaggata atgacaagct gaagaagctg atcaacaagt ctcccgagaa gctgctgatg     3180

taccaccacg atcctcagac atatcagaag ctgaagctga tcatggagca gtacggcgac     3240

gagaagaacc cactgtataa gtactatgag gagacaggca actacctgac aaagtatagc     3300

aagaaggata atggccccgt gatcaagaag atcaagtact atggcaacaa gctgaatgcc     3360

cacctggaca tcaccgacga ttaccctaac tctcgcaata aggtggtgaa gctgagcctg     3420

aagccatacc ggttcgacgt gtacctggac aacggcgtgt ataagtttgt gacagtgaag     3480

aatctggatg tgatcaagaa ggagaactac tatgaggtga acagcaagtg ctacgaggag     3540

gccaagaagc tgaagaagat cagcaaccag gccgagttca tcgcctcttt ttacaacaat     3600

gacctgatca agatcaatgg cgagctgtat agagtgatcg gcgtgaacaa tgatctgctg     3660

aacagaatcg aagtgaatat gatcgacatc acctacaggg agtatctgga gaacatgaat     3720

gataagaggc cccctcgcat catcaagacc atcgcctcta agacacagag catcaagaag     3780

tacagcacag acatcctggg gaacctgtat gaagtcaaga gcaagaaaca tcctcagatt     3840

atcaagaaag gctctggtgg ttctaagcga cctgccgcca caaagaaggc tggacaggct     3900

aagaagaaga aagattacaa agacgatgac gataagggat ccggcgcaac aaacttctct     3960

ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgatggccaa gcctttgtct     4020

caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct     4080

gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc     4140

aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct     4200

gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc     4260

ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc     4320

atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct     4380

ggttatgtgt gggagggcta a                                               4401


<210>  139
<211>  4401
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nSaCas9 G129 miniABEMax V82G

<400>  139
atgaagcgaa attacattct ggggctggcc attggcatta catcagtggg ctatggcatc       60

attgactacg agacaaggga cgtgatcgac gccggcgtga gactgttcaa ggaggccaac      120

gtggagaaca atgagggccg gagatccaag aggggagcaa ggcgcctgaa gcggagaagg      180

cgccacagaa tccagagagt gaagaagctg ctgttcgatt acaacctgct gaccgaccac      240

tccgagctgt ctggcatcaa tccttatgag gccagagtga agggcctgtc ccagaagctg      300

tctgaggagg agtttagcgc cgccctgctg cacctggcaa agaggagagg cgtgcacaac      360

gtgaatgagg tggaggagga caccggcccg aagaagaaac gtaaggtggg tagcagcggt      420

agcccgaaaa agaagcgcaa agtgggcagc gacgcgctgg acgatttcga tctggacatg      480

ctgggcagcg atgcgctgga tgatttcggc ggtggcagct ctgaggtgga gttttcccac      540

gagtactgga tgagacatgc cctgaccctg gccaagaggg cacgcgatga gagggaggtg      600

cctgtgggag ccgtgctggt gctgaacaat agagtgatcg gcgagggctg gaacagagcc      660

atcggcctgc acgacccaac agcccatgcc gaaattatgg ccctgagaca gggcggcctg      720

gtcatgcaga actacagact gattgacgcc accctgtacg gcacattcga gccttgcgtg      780

atgtgcgccg gcgccatgat ccactctagg atcggccgcg tggtgtttgg cgtgaggaac      840

gcaaaaaccg gcgccgcagg ctccctgatg gacgtgctgc actaccccgg catgaatcac      900

cgcgtcgaaa ttaccgaggg aatcctggca gatgaatgtg ccgccctgct gtgctatttc      960

tttcggatgc ctagacaggt gttcaatgct cagaagaagg cccagagctc caccgacggc     1020

ggtagcggcg gtagcggtgg tagcggcggc agcggtggta gcggtggcag cggtggtggc     1080

aacgagctgt ccacaaagga gcagatcagc cgcaattcca aggccctgga ggagaagtat     1140

gtggccgagc tgcagctgga gcggctgaag aaggatggcg aggtgagggg ctccatcaat     1200

cgcttcaaga cctctgacta cgtgaaggag gccaagcagc tgctgaaggt gcagaaggcc     1260

taccaccagc tggatcagtc ctttatcgat acatatatcg acctgctgga gacaaggcgc     1320

acatactatg agggaccagg agagggctct cccttcggct ggaaggacat caaggagtgg     1380

tacgagatgc tgatgggcca ctgcacctat tttccagagg agctgagaag cgtgaagtac     1440

gcctataacg ccgatctgta caacgccctg aatgacctga acaacctggt catcaccagg     1500

gatgagaacg agaagctgga gtactatgag aagttccaga tcatcgagaa cgtgttcaag     1560

cagaagaaga agcctacact gaagcagatc gccaaggaga tcctggtgaa cgaggaggac     1620

atcaagggct accgcgtgac ctccacaggc aagccagagt tcaccaatct gaaggtgtat     1680

cacgatatca aggacatcac agcccggaag gagatcatcg agaacgccga gctgctggat     1740

cagatcgcca agatcctgac catctatcag agctccgagg acatccagga ggagctgacc     1800

aacctgaata gcgagctgac acaggaggag atcgagcaga tcagcaatct gaagggctac     1860

accggcacac acaacctgag cctgaaggcc atcaatctga tcctggatga gctgtggcac     1920

acaaacgaca atcagatcgc catctttaac cggctgaagc tggtgccaaa gaaggtggac     1980

ctgtcccagc agaaggagat cccaaccaca ctggtggacg atttcatcct gtctcccgtg     2040

gtgaagcgga gcttcatcca gagcatcaaa gtgatcaacg ccatcatcaa gaagtacggc     2100

ctgcccaatg atatcatcat cgagctggcc agggagaaga actccaagga cgcccagaag     2160

atgatcaatg agatgcagaa gaggaaccgc cagaccaatg agcggatcga ggagatcatc     2220

agaaccacag gcaaggagaa cgccaagtac ctgatcgaga agatcaagct gcacgatatg     2280

caggagggca agtgtctgta ttctctggag gccatccctc tggaggacct gctgaacaat     2340

ccattcaact acgaggtgga tcacatcatc ccccggagcg tgagcttcga caattctttt     2400

aacaataagg tgctggtgaa gcaggaggag aacagcaaga agggcaatag gacccctttc     2460

cagtacctgt ctagctccga ttctaagatc agctacgaga cattcaagaa gcacatcctg     2520

aatctggcca agggcaaggg ccgcatcagc aagaccaaga aggagtacct gctggaggag     2580

cgggacatca acagattctc cgtgcagaag gacttcatca accggaatct ggtggacacc     2640

agatacgcca cacgcggcct gatgaatctg ctgcggtctt atttcagagt gaacaatctg     2700

gatgtgaagg tgaagagcat caacggcggc ttcacctcct ttctgcggag aaagtggaag     2760

tttaagaagg agcgcaacaa gggctataag caccacgccg aggatgccct gatcatcgcc     2820

aatgccgact tcatctttaa ggagtggaag aagctggaca aggccaagaa agtgatggag     2880

aaccagatgt tcgaggagaa gcaggccgag agcatgcccg agatcgagac agagcaggag     2940

tacaaggaga ttttcatcac acctcaccag atcaagcaca tcaaggactt caaggactac     3000

aagtattctc acagggtgga taagaagccc aaccgcgagc tgatcaatga caccctgtat     3060

agcacacgga aggacgataa gggcaatacc ctgatcgtga acaatctgaa cggcctgtac     3120

gacaaggata atgacaagct gaagaagctg atcaacaagt ctcccgagaa gctgctgatg     3180

taccaccacg atcctcagac atatcagaag ctgaagctga tcatggagca gtacggcgac     3240

gagaagaacc cactgtataa gtactatgag gagacaggca actacctgac aaagtatagc     3300

aagaaggata atggccccgt gatcaagaag atcaagtact atggcaacaa gctgaatgcc     3360

cacctggaca tcaccgacga ttaccctaac tctcgcaata aggtggtgaa gctgagcctg     3420

aagccatacc ggttcgacgt gtacctggac aacggcgtgt ataagtttgt gacagtgaag     3480

aatctggatg tgatcaagaa ggagaactac tatgaggtga acagcaagtg ctacgaggag     3540

gccaagaagc tgaagaagat cagcaaccag gccgagttca tcgcctcttt ttacaacaat     3600

gacctgatca agatcaatgg cgagctgtat agagtgatcg gcgtgaacaa tgatctgctg     3660

aacagaatcg aagtgaatat gatcgacatc acctacaggg agtatctgga gaacatgaat     3720

gataagaggc cccctcgcat catcaagacc atcgcctcta agacacagag catcaagaag     3780

tacagcacag acatcctggg gaacctgtat gaagtcaaga gcaagaaaca tcctcagatt     3840

atcaagaaag gctctggtgg ttctaagcga cctgccgcca caaagaaggc tggacaggct     3900

aagaagaaga aagattacaa agacgatgac gataagggat ccggcgcaac aaacttctct     3960

ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgatggccaa gcctttgtct     4020

caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct     4080

gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc     4140

aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct     4200

gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc     4260

ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc     4320

atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct     4380

ggttatgtgt gggagggcta a                                               4401


<210>  140
<211>  4401
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nSaCas9 H119 miniABEMax V82G

<400>  140
atgaagcgaa attacattct ggggctggcc attggcatta catcagtggg ctatggcatc       60

attgactacg agacaaggga cgtgatcgac gccggcgtga gactgttcaa ggaggccaac      120

gtggagaaca atgagggccg gagatccaag aggggagcaa ggcgcctgaa gcggagaagg      180

cgccacagaa tccagagagt gaagaagctg ctgttcgatt acaacctgct gaccgaccac      240

tccgagctgt ctggcatcaa tccttatgag gccagagtga agggcctgtc ccagaagctg      300

tctgaggagg agtttagcgc cgccctgctg cacctggcaa agaggagagg cgtgcacccg      360

aagaagaaac gtaaggtggg tagcagcggt agcccgaaaa agaagcgcaa agtgggcagc      420

gacgcgctgg acgatttcga tctggacatg ctgggcagcg atgcgctgga tgatttcggc      480

ggtggcagct ctgaggtgga gttttcccac gagtactgga tgagacatgc cctgaccctg      540

gccaagaggg cacgcgatga gagggaggtg cctgtgggag ccgtgctggt gctgaacaat      600

agagtgatcg gcgagggctg gaacagagcc atcggcctgc acgacccaac agcccatgcc      660

gaaattatgg ccctgagaca gggcggcctg gtcatgcaga actacagact gattgacgcc      720

accctgtacg gcacattcga gccttgcgtg atgtgcgccg gcgccatgat ccactctagg      780

atcggccgcg tggtgtttgg cgtgaggaac gcaaaaaccg gcgccgcagg ctccctgatg      840

gacgtgctgc actaccccgg catgaatcac cgcgtcgaaa ttaccgaggg aatcctggca      900

gatgaatgtg ccgccctgct gtgctatttc tttcggatgc ctagacaggt gttcaatgct      960

cagaagaagg cccagagctc caccgacggc ggtagcggcg gtagcggtgg tagcggcggc     1020

agcggtggta gcggtggcag cggtggtcac aacgtgaatg aggtggagga ggacaccggc     1080

aacgagctgt ccacaaagga gcagatcagc cgcaattcca aggccctgga ggagaagtat     1140

gtggccgagc tgcagctgga gcggctgaag aaggatggcg aggtgagggg ctccatcaat     1200

cgcttcaaga cctctgacta cgtgaaggag gccaagcagc tgctgaaggt gcagaaggcc     1260

taccaccagc tggatcagtc ctttatcgat acatatatcg acctgctgga gacaaggcgc     1320

acatactatg agggaccagg agagggctct cccttcggct ggaaggacat caaggagtgg     1380

tacgagatgc tgatgggcca ctgcacctat tttccagagg agctgagaag cgtgaagtac     1440

gcctataacg ccgatctgta caacgccctg aatgacctga acaacctggt catcaccagg     1500

gatgagaacg agaagctgga gtactatgag aagttccaga tcatcgagaa cgtgttcaag     1560

cagaagaaga agcctacact gaagcagatc gccaaggaga tcctggtgaa cgaggaggac     1620

atcaagggct accgcgtgac ctccacaggc aagccagagt tcaccaatct gaaggtgtat     1680

cacgatatca aggacatcac agcccggaag gagatcatcg agaacgccga gctgctggat     1740

cagatcgcca agatcctgac catctatcag agctccgagg acatccagga ggagctgacc     1800

aacctgaata gcgagctgac acaggaggag atcgagcaga tcagcaatct gaagggctac     1860

accggcacac acaacctgag cctgaaggcc atcaatctga tcctggatga gctgtggcac     1920

acaaacgaca atcagatcgc catctttaac cggctgaagc tggtgccaaa gaaggtggac     1980

ctgtcccagc agaaggagat cccaaccaca ctggtggacg atttcatcct gtctcccgtg     2040

gtgaagcgga gcttcatcca gagcatcaaa gtgatcaacg ccatcatcaa gaagtacggc     2100

ctgcccaatg atatcatcat cgagctggcc agggagaaga actccaagga cgcccagaag     2160

atgatcaatg agatgcagaa gaggaaccgc cagaccaatg agcggatcga ggagatcatc     2220

agaaccacag gcaaggagaa cgccaagtac ctgatcgaga agatcaagct gcacgatatg     2280

caggagggca agtgtctgta ttctctggag gccatccctc tggaggacct gctgaacaat     2340

ccattcaact acgaggtgga tcacatcatc ccccggagcg tgagcttcga caattctttt     2400

aacaataagg tgctggtgaa gcaggaggag aacagcaaga agggcaatag gacccctttc     2460

cagtacctgt ctagctccga ttctaagatc agctacgaga cattcaagaa gcacatcctg     2520

aatctggcca agggcaaggg ccgcatcagc aagaccaaga aggagtacct gctggaggag     2580

cgggacatca acagattctc cgtgcagaag gacttcatca accggaatct ggtggacacc     2640

agatacgcca cacgcggcct gatgaatctg ctgcggtctt atttcagagt gaacaatctg     2700

gatgtgaagg tgaagagcat caacggcggc ttcacctcct ttctgcggag aaagtggaag     2760

tttaagaagg agcgcaacaa gggctataag caccacgccg aggatgccct gatcatcgcc     2820

aatgccgact tcatctttaa ggagtggaag aagctggaca aggccaagaa agtgatggag     2880

aaccagatgt tcgaggagaa gcaggccgag agcatgcccg agatcgagac agagcaggag     2940

tacaaggaga ttttcatcac acctcaccag atcaagcaca tcaaggactt caaggactac     3000

aagtattctc acagggtgga taagaagccc aaccgcgagc tgatcaatga caccctgtat     3060

agcacacgga aggacgataa gggcaatacc ctgatcgtga acaatctgaa cggcctgtac     3120

gacaaggata atgacaagct gaagaagctg atcaacaagt ctcccgagaa gctgctgatg     3180

taccaccacg atcctcagac atatcagaag ctgaagctga tcatggagca gtacggcgac     3240

gagaagaacc cactgtataa gtactatgag gagacaggca actacctgac aaagtatagc     3300

aagaaggata atggccccgt gatcaagaag atcaagtact atggcaacaa gctgaatgcc     3360

cacctggaca tcaccgacga ttaccctaac tctcgcaata aggtggtgaa gctgagcctg     3420

aagccatacc ggttcgacgt gtacctggac aacggcgtgt ataagtttgt gacagtgaag     3480

aatctggatg tgatcaagaa ggagaactac tatgaggtga acagcaagtg ctacgaggag     3540

gccaagaagc tgaagaagat cagcaaccag gccgagttca tcgcctcttt ttacaacaat     3600

gacctgatca agatcaatgg cgagctgtat agagtgatcg gcgtgaacaa tgatctgctg     3660

aacagaatcg aagtgaatat gatcgacatc acctacaggg agtatctgga gaacatgaat     3720

gataagaggc cccctcgcat catcaagacc atcgcctcta agacacagag catcaagaag     3780

tacagcacag acatcctggg gaacctgtat gaagtcaaga gcaagaaaca tcctcagatt     3840

atcaagaaag gctctggtgg ttctaagcga cctgccgcca caaagaaggc tggacaggct     3900

aagaagaaga aagattacaa agacgatgac gataagggat ccggcgcaac aaacttctct     3960

ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgatggccaa gcctttgtct     4020

caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct     4080

gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc     4140

aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct     4200

gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc     4260

ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc     4320

atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct     4380

ggttatgtgt gggagggcta a                                               4401


<210>  141
<211>  4401
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nSaCas9 M732 miniABEMax V82G

<400>  141
atgaagcgaa attacattct ggggctggcc attggcatta catcagtggg ctatggcatc       60

attgactacg agacaaggga cgtgatcgac gccggcgtga gactgttcaa ggaggccaac      120

gtggagaaca atgagggccg gagatccaag aggggagcaa ggcgcctgaa gcggagaagg      180

cgccacagaa tccagagagt gaagaagctg ctgttcgatt acaacctgct gaccgaccac      240

tccgagctgt ctggcatcaa tccttatgag gccagagtga agggcctgtc ccagaagctg      300

tctgaggagg agtttagcgc cgccctgctg cacctggcaa agaggagagg cgtgcacaac      360

gtgaatgagg tggaggagga caccggcaac gagctgtcca caaaggagca gatcagccgc      420

aattccaagg ccctggagga gaagtatgtg gccgagctgc agctggagcg gctgaagaag      480

gatggcgagg tgaggggctc catcaatcgc ttcaagacct ctgactacgt gaaggaggcc      540

aagcagctgc tgaaggtgca gaaggcctac caccagctgg atcagtcctt tatcgataca      600

tatatcgacc tgctggagac aaggcgcaca tactatgagg gaccaggaga gggctctccc      660

ttcggctgga aggacatcaa ggagtggtac gagatgctga tgggccactg cacctatttt      720

ccagaggagc tgagaagcgt gaagtacgcc tataacgccg atctgtacaa cgccctgaat      780

gacctgaaca acctggtcat caccagggat gagaacgaga agctggagta ctatgagaag      840

ttccagatca tcgagaacgt gttcaagcag aagaagaagc ctacactgaa gcagatcgcc      900

aaggagatcc tggtgaacga ggaggacatc aagggctacc gcgtgacctc cacaggcaag      960

ccagagttca ccaatctgaa ggtgtatcac gatatcaagg acatcacagc ccggaaggag     1020

atcatcgaga acgccgagct gctggatcag atcgccaaga tcctgaccat ctatcagagc     1080

tccgaggaca tccaggagga gctgaccaac ctgaatagcg agctgacaca ggaggagatc     1140

gagcagatca gcaatctgaa gggctacacc ggcacacaca acctgagcct gaaggccatc     1200

aatctgatcc tggatgagct gtggcacaca aacgacaatc agatcgccat ctttaaccgg     1260

ctgaagctgg tgccaaagaa ggtggacctg tcccagcaga aggagatccc aaccacactg     1320

gtggacgatt tcatcctgtc tcccgtggtg aagcggagct tcatccagag catcaaagtg     1380

atcaacgcca tcatcaagaa gtacggcctg cccaatgata tcatcatcga gctggccagg     1440

gagaagaact ccaaggacgc ccagaagatg atcaatgaga tgcagaagag gaaccgccag     1500

accaatgagc ggatcgagga gatcatcaga accacaggca aggagaacgc caagtacctg     1560

atcgagaaga tcaagctgca cgatatgcag gagggcaagt gtctgtattc tctggaggcc     1620

atccctctgg aggacctgct gaacaatcca ttcaactacg aggtggatca catcatcccc     1680

cggagcgtga gcttcgacaa ttcttttaac aataaggtgc tggtgaagca ggaggagaac     1740

agcaagaagg gcaataggac ccctttccag tacctgtcta gctccgattc taagatcagc     1800

tacgagacat tcaagaagca catcctgaat ctggccaagg gcaagggccg catcagcaag     1860

accaagaagg agtacctgct ggaggagcgg gacatcaaca gattctccgt gcagaaggac     1920

ttcatcaacc ggaatctggt ggacaccaga tacgccacac gcggcctgat gaatctgctg     1980

cggtcttatt tcagagtgaa caatctggat gtgaaggtga agagcatcaa cggcggcttc     2040

acctcctttc tgcggagaaa gtggaagttt aagaaggagc gcaacaaggg ctataagcac     2100

cacgccgagg atgccctgat catcgccaat gccgacttca tctttaagga gtggaagaag     2160

ctggacaagg ccaagaaagt gatggagaac cagatgccga agaagaaacg taaggtgggt     2220

agcagcggta gcccgaaaaa gaagcgcaaa gtgggcagcg acgcgctgga cgatttcgat     2280

ctggacatgc tgggcagcga tgcgctggat gatttcggcg gtggcagctc tgaggtggag     2340

ttttcccacg agtactggat gagacatgcc ctgaccctgg ccaagagggc acgcgatgag     2400

agggaggtgc ctgtgggagc cgtgctggtg ctgaacaata gagtgatcgg cgagggctgg     2460

aacagagcca tcggcctgca cgacccaaca gcccatgccg aaattatggc cctgagacag     2520

ggcggcctgg tcatgcagaa ctacagactg attgacgcca ccctgtacgg cacattcgag     2580

ccttgcgtga tgtgcgccgg cgccatgatc cactctagga tcggccgcgt ggtgtttggc     2640

gtgaggaacg caaaaaccgg cgccgcaggc tccctgatgg acgtgctgca ctaccccggc     2700

atgaatcacc gcgtcgaaat taccgaggga atcctggcag atgaatgtgc cgccctgctg     2760

tgctatttct ttcggatgcc tagacaggtg ttcaatgctc agaagaaggc ccagagctcc     2820

accgacggcg gtagcggcgg tagcggtggt agcggcggca gcggtggtag cggtggcagc     2880

ggtggtatgt tcgaggagaa gcaggccgag agcatgcccg agatcgagac agagcaggag     2940

tacaaggaga ttttcatcac acctcaccag atcaagcaca tcaaggactt caaggactac     3000

aagtattctc acagggtgga taagaagccc aaccgcgagc tgatcaatga caccctgtat     3060

agcacacgga aggacgataa gggcaatacc ctgatcgtga acaatctgaa cggcctgtac     3120

gacaaggata atgacaagct gaagaagctg atcaacaagt ctcccgagaa gctgctgatg     3180

taccaccacg atcctcagac atatcagaag ctgaagctga tcatggagca gtacggcgac     3240

gagaagaacc cactgtataa gtactatgag gagacaggca actacctgac aaagtatagc     3300

aagaaggata atggccccgt gatcaagaag atcaagtact atggcaacaa gctgaatgcc     3360

cacctggaca tcaccgacga ttaccctaac tctcgcaata aggtggtgaa gctgagcctg     3420

aagccatacc ggttcgacgt gtacctggac aacggcgtgt ataagtttgt gacagtgaag     3480

aatctggatg tgatcaagaa ggagaactac tatgaggtga acagcaagtg ctacgaggag     3540

gccaagaagc tgaagaagat cagcaaccag gccgagttca tcgcctcttt ttacaacaat     3600

gacctgatca agatcaatgg cgagctgtat agagtgatcg gcgtgaacaa tgatctgctg     3660

aacagaatcg aagtgaatat gatcgacatc acctacaggg agtatctgga gaacatgaat     3720

gataagaggc cccctcgcat catcaagacc atcgcctcta agacacagag catcaagaag     3780

tacagcacag acatcctggg gaacctgtat gaagtcaaga gcaagaaaca tcctcagatt     3840

atcaagaaag gctctggtgg ttctaagcga cctgccgcca caaagaaggc tggacaggct     3900

aagaagaaga aagattacaa agacgatgac gataagggat ccggcgcaac aaacttctct     3960

ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgatggccaa gcctttgtct     4020

caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct     4080

gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc     4140

aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct     4200

gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc     4260

ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc     4320

atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct     4380

ggttatgtgt gggagggcta a                                               4401


<210>  142
<211>  4401
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nSaCas9 M741 miniABEMax V82G

<400>  142
atgaagcgaa attacattct ggggctggcc attggcatta catcagtggg ctatggcatc       60

attgactacg agacaaggga cgtgatcgac gccggcgtga gactgttcaa ggaggccaac      120

gtggagaaca atgagggccg gagatccaag aggggagcaa ggcgcctgaa gcggagaagg      180

cgccacagaa tccagagagt gaagaagctg ctgttcgatt acaacctgct gaccgaccac      240

tccgagctgt ctggcatcaa tccttatgag gccagagtga agggcctgtc ccagaagctg      300

tctgaggagg agtttagcgc cgccctgctg cacctggcaa agaggagagg cgtgcacaac      360

gtgaatgagg tggaggagga caccggcaac gagctgtcca caaaggagca gatcagccgc      420

aattccaagg ccctggagga gaagtatgtg gccgagctgc agctggagcg gctgaagaag      480

gatggcgagg tgaggggctc catcaatcgc ttcaagacct ctgactacgt gaaggaggcc      540

aagcagctgc tgaaggtgca gaaggcctac caccagctgg atcagtcctt tatcgataca      600

tatatcgacc tgctggagac aaggcgcaca tactatgagg gaccaggaga gggctctccc      660

ttcggctgga aggacatcaa ggagtggtac gagatgctga tgggccactg cacctatttt      720

ccagaggagc tgagaagcgt gaagtacgcc tataacgccg atctgtacaa cgccctgaat      780

gacctgaaca acctggtcat caccagggat gagaacgaga agctggagta ctatgagaag      840

ttccagatca tcgagaacgt gttcaagcag aagaagaagc ctacactgaa gcagatcgcc      900

aaggagatcc tggtgaacga ggaggacatc aagggctacc gcgtgacctc cacaggcaag      960

ccagagttca ccaatctgaa ggtgtatcac gatatcaagg acatcacagc ccggaaggag     1020

atcatcgaga acgccgagct gctggatcag atcgccaaga tcctgaccat ctatcagagc     1080

tccgaggaca tccaggagga gctgaccaac ctgaatagcg agctgacaca ggaggagatc     1140

gagcagatca gcaatctgaa gggctacacc ggcacacaca acctgagcct gaaggccatc     1200

aatctgatcc tggatgagct gtggcacaca aacgacaatc agatcgccat ctttaaccgg     1260

ctgaagctgg tgccaaagaa ggtggacctg tcccagcaga aggagatccc aaccacactg     1320

gtggacgatt tcatcctgtc tcccgtggtg aagcggagct tcatccagag catcaaagtg     1380

atcaacgcca tcatcaagaa gtacggcctg cccaatgata tcatcatcga gctggccagg     1440

gagaagaact ccaaggacgc ccagaagatg atcaatgaga tgcagaagag gaaccgccag     1500

accaatgagc ggatcgagga gatcatcaga accacaggca aggagaacgc caagtacctg     1560

atcgagaaga tcaagctgca cgatatgcag gagggcaagt gtctgtattc tctggaggcc     1620

atccctctgg aggacctgct gaacaatcca ttcaactacg aggtggatca catcatcccc     1680

cggagcgtga gcttcgacaa ttcttttaac aataaggtgc tggtgaagca ggaggagaac     1740

agcaagaagg gcaataggac ccctttccag tacctgtcta gctccgattc taagatcagc     1800

tacgagacat tcaagaagca catcctgaat ctggccaagg gcaagggccg catcagcaag     1860

accaagaagg agtacctgct ggaggagcgg gacatcaaca gattctccgt gcagaaggac     1920

ttcatcaacc ggaatctggt ggacaccaga tacgccacac gcggcctgat gaatctgctg     1980

cggtcttatt tcagagtgaa caatctggat gtgaaggtga agagcatcaa cggcggcttc     2040

acctcctttc tgcggagaaa gtggaagttt aagaaggagc gcaacaaggg ctataagcac     2100

cacgccgagg atgccctgat catcgccaat gccgacttca tctttaagga gtggaagaag     2160

ctggacaagg ccaagaaagt gatggagaac cagatgttcg aggagaagca ggccgagagc     2220

atgccgaaga agaaacgtaa ggtgggtagc agcggtagcc cgaaaaagaa gcgcaaagtg     2280

ggcagcgacg cgctggacga tttcgatctg gacatgctgg gcagcgatgc gctggatgat     2340

ttcggcggtg gcagctctga ggtggagttt tcccacgagt actggatgag acatgccctg     2400

accctggcca agagggcacg cgatgagagg gaggtgcctg tgggagccgt gctggtgctg     2460

aacaatagag tgatcggcga gggctggaac agagccatcg gcctgcacga cccaacagcc     2520

catgccgaaa ttatggccct gagacagggc ggcctggtca tgcagaacta cagactgatt     2580

gacgccaccc tgtacggcac attcgagcct tgcgtgatgt gcgccggcgc catgatccac     2640

tctaggatcg gccgcgtggt gtttggcgtg aggaacgcaa aaaccggcgc cgcaggctcc     2700

ctgatggacg tgctgcacta ccccggcatg aatcaccgcg tcgaaattac cgagggaatc     2760

ctggcagatg aatgtgccgc cctgctgtgc tatttctttc ggatgcctag acaggtgttc     2820

aatgctcaga agaaggccca gagctccacc gacggcggta gcggcggtag cggtggtagc     2880

ggcggcagcg gtggtagcgg tggcagcggt ggtatgcccg agatcgagac agagcaggag     2940

tacaaggaga ttttcatcac acctcaccag atcaagcaca tcaaggactt caaggactac     3000

aagtattctc acagggtgga taagaagccc aaccgcgagc tgatcaatga caccctgtat     3060

agcacacgga aggacgataa gggcaatacc ctgatcgtga acaatctgaa cggcctgtac     3120

gacaaggata atgacaagct gaagaagctg atcaacaagt ctcccgagaa gctgctgatg     3180

taccaccacg atcctcagac atatcagaag ctgaagctga tcatggagca gtacggcgac     3240

gagaagaacc cactgtataa gtactatgag gagacaggca actacctgac aaagtatagc     3300

aagaaggata atggccccgt gatcaagaag atcaagtact atggcaacaa gctgaatgcc     3360

cacctggaca tcaccgacga ttaccctaac tctcgcaata aggtggtgaa gctgagcctg     3420

aagccatacc ggttcgacgt gtacctggac aacggcgtgt ataagtttgt gacagtgaag     3480

aatctggatg tgatcaagaa ggagaactac tatgaggtga acagcaagtg ctacgaggag     3540

gccaagaagc tgaagaagat cagcaaccag gccgagttca tcgcctcttt ttacaacaat     3600

gacctgatca agatcaatgg cgagctgtat agagtgatcg gcgtgaacaa tgatctgctg     3660

aacagaatcg aagtgaatat gatcgacatc acctacaggg agtatctgga gaacatgaat     3720

gataagaggc cccctcgcat catcaagacc atcgcctcta agacacagag catcaagaag     3780

tacagcacag acatcctggg gaacctgtat gaagtcaaga gcaagaaaca tcctcagatt     3840

atcaagaaag gctctggtgg ttctaagcga cctgccgcca caaagaaggc tggacaggct     3900

aagaagaaga aagattacaa agacgatgac gataagggat ccggcgcaac aaacttctct     3960

ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgatggccaa gcctttgtct     4020

caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct     4080

gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc     4140

aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct     4200

gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc     4260

ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc     4320

atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct     4380

ggttatgtgt gggagggcta a                                               4401


<210>  143
<211>  4401
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nSaCas9 N120 miniABEMax V82G

<400>  143
atgaagcgaa attacattct ggggctggcc attggcatta catcagtggg ctatggcatc       60

attgactacg agacaaggga cgtgatcgac gccggcgtga gactgttcaa ggaggccaac      120

gtggagaaca atgagggccg gagatccaag aggggagcaa ggcgcctgaa gcggagaagg      180

cgccacagaa tccagagagt gaagaagctg ctgttcgatt acaacctgct gaccgaccac      240

tccgagctgt ctggcatcaa tccttatgag gccagagtga agggcctgtc ccagaagctg      300

tctgaggagg agtttagcgc cgccctgctg cacctggcaa agaggagagg cgtgcacaac      360

ccgaagaaga aacgtaaggt gggtagcagc ggtagcccga aaaagaagcg caaagtgggc      420

agcgacgcgc tggacgattt cgatctggac atgctgggca gcgatgcgct ggatgatttc      480

ggcggtggca gctctgaggt ggagttttcc cacgagtact ggatgagaca tgccctgacc      540

ctggccaaga gggcacgcga tgagagggag gtgcctgtgg gagccgtgct ggtgctgaac      600

aatagagtga tcggcgaggg ctggaacaga gccatcggcc tgcacgaccc aacagcccat      660

gccgaaatta tggccctgag acagggcggc ctggtcatgc agaactacag actgattgac      720

gccaccctgt acggcacatt cgagccttgc gtgatgtgcg ccggcgccat gatccactct      780

aggatcggcc gcgtggtgtt tggcgtgagg aacgcaaaaa ccggcgccgc aggctccctg      840

atggacgtgc tgcactaccc cggcatgaat caccgcgtcg aaattaccga gggaatcctg      900

gcagatgaat gtgccgccct gctgtgctat ttctttcgga tgcctagaca ggtgttcaat      960

gctcagaaga aggcccagag ctccaccgac ggcggtagcg gcggtagcgg tggtagcggc     1020

ggcagcggtg gtagcggtgg cagcggtggt aacgtgaatg aggtggagga ggacaccggc     1080

aacgagctgt ccacaaagga gcagatcagc cgcaattcca aggccctgga ggagaagtat     1140

gtggccgagc tgcagctgga gcggctgaag aaggatggcg aggtgagggg ctccatcaat     1200

cgcttcaaga cctctgacta cgtgaaggag gccaagcagc tgctgaaggt gcagaaggcc     1260

taccaccagc tggatcagtc ctttatcgat acatatatcg acctgctgga gacaaggcgc     1320

acatactatg agggaccagg agagggctct cccttcggct ggaaggacat caaggagtgg     1380

tacgagatgc tgatgggcca ctgcacctat tttccagagg agctgagaag cgtgaagtac     1440

gcctataacg ccgatctgta caacgccctg aatgacctga acaacctggt catcaccagg     1500

gatgagaacg agaagctgga gtactatgag aagttccaga tcatcgagaa cgtgttcaag     1560

cagaagaaga agcctacact gaagcagatc gccaaggaga tcctggtgaa cgaggaggac     1620

atcaagggct accgcgtgac ctccacaggc aagccagagt tcaccaatct gaaggtgtat     1680

cacgatatca aggacatcac agcccggaag gagatcatcg agaacgccga gctgctggat     1740

cagatcgcca agatcctgac catctatcag agctccgagg acatccagga ggagctgacc     1800

aacctgaata gcgagctgac acaggaggag atcgagcaga tcagcaatct gaagggctac     1860

accggcacac acaacctgag cctgaaggcc atcaatctga tcctggatga gctgtggcac     1920

acaaacgaca atcagatcgc catctttaac cggctgaagc tggtgccaaa gaaggtggac     1980

ctgtcccagc agaaggagat cccaaccaca ctggtggacg atttcatcct gtctcccgtg     2040

gtgaagcgga gcttcatcca gagcatcaaa gtgatcaacg ccatcatcaa gaagtacggc     2100

ctgcccaatg atatcatcat cgagctggcc agggagaaga actccaagga cgcccagaag     2160

atgatcaatg agatgcagaa gaggaaccgc cagaccaatg agcggatcga ggagatcatc     2220

agaaccacag gcaaggagaa cgccaagtac ctgatcgaga agatcaagct gcacgatatg     2280

caggagggca agtgtctgta ttctctggag gccatccctc tggaggacct gctgaacaat     2340

ccattcaact acgaggtgga tcacatcatc ccccggagcg tgagcttcga caattctttt     2400

aacaataagg tgctggtgaa gcaggaggag aacagcaaga agggcaatag gacccctttc     2460

cagtacctgt ctagctccga ttctaagatc agctacgaga cattcaagaa gcacatcctg     2520

aatctggcca agggcaaggg ccgcatcagc aagaccaaga aggagtacct gctggaggag     2580

cgggacatca acagattctc cgtgcagaag gacttcatca accggaatct ggtggacacc     2640

agatacgcca cacgcggcct gatgaatctg ctgcggtctt atttcagagt gaacaatctg     2700

gatgtgaagg tgaagagcat caacggcggc ttcacctcct ttctgcggag aaagtggaag     2760

tttaagaagg agcgcaacaa gggctataag caccacgccg aggatgccct gatcatcgcc     2820

aatgccgact tcatctttaa ggagtggaag aagctggaca aggccaagaa agtgatggag     2880

aaccagatgt tcgaggagaa gcaggccgag agcatgcccg agatcgagac agagcaggag     2940

tacaaggaga ttttcatcac acctcaccag atcaagcaca tcaaggactt caaggactac     3000

aagtattctc acagggtgga taagaagccc aaccgcgagc tgatcaatga caccctgtat     3060

agcacacgga aggacgataa gggcaatacc ctgatcgtga acaatctgaa cggcctgtac     3120

gacaaggata atgacaagct gaagaagctg atcaacaagt ctcccgagaa gctgctgatg     3180

taccaccacg atcctcagac atatcagaag ctgaagctga tcatggagca gtacggcgac     3240

gagaagaacc cactgtataa gtactatgag gagacaggca actacctgac aaagtatagc     3300

aagaaggata atggccccgt gatcaagaag atcaagtact atggcaacaa gctgaatgcc     3360

cacctggaca tcaccgacga ttaccctaac tctcgcaata aggtggtgaa gctgagcctg     3420

aagccatacc ggttcgacgt gtacctggac aacggcgtgt ataagtttgt gacagtgaag     3480

aatctggatg tgatcaagaa ggagaactac tatgaggtga acagcaagtg ctacgaggag     3540

gccaagaagc tgaagaagat cagcaaccag gccgagttca tcgcctcttt ttacaacaat     3600

gacctgatca agatcaatgg cgagctgtat agagtgatcg gcgtgaacaa tgatctgctg     3660

aacagaatcg aagtgaatat gatcgacatc acctacaggg agtatctgga gaacatgaat     3720

gataagaggc cccctcgcat catcaagacc atcgcctcta agacacagag catcaagaag     3780

tacagcacag acatcctggg gaacctgtat gaagtcaaga gcaagaaaca tcctcagatt     3840

atcaagaaag gctctggtgg ttctaagcga cctgccgcca caaagaaggc tggacaggct     3900

aagaagaaga aagattacaa agacgatgac gataagggat ccggcgcaac aaacttctct     3960

ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgatggccaa gcctttgtct     4020

caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct     4080

gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc     4140

aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct     4200

gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc     4260

ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc     4320

atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct     4380

ggttatgtgt gggagggcta a                                               4401


<210>  144
<211>  4401
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nSaCas9 P742 miniABEMax V82G

<400>  144
atgaagcgaa attacattct ggggctggcc attggcatta catcagtggg ctatggcatc       60

attgactacg agacaaggga cgtgatcgac gccggcgtga gactgttcaa ggaggccaac      120

gtggagaaca atgagggccg gagatccaag aggggagcaa ggcgcctgaa gcggagaagg      180

cgccacagaa tccagagagt gaagaagctg ctgttcgatt acaacctgct gaccgaccac      240

tccgagctgt ctggcatcaa tccttatgag gccagagtga agggcctgtc ccagaagctg      300

tctgaggagg agtttagcgc cgccctgctg cacctggcaa agaggagagg cgtgcacaac      360

gtgaatgagg tggaggagga caccggcaac gagctgtcca caaaggagca gatcagccgc      420

aattccaagg ccctggagga gaagtatgtg gccgagctgc agctggagcg gctgaagaag      480

gatggcgagg tgaggggctc catcaatcgc ttcaagacct ctgactacgt gaaggaggcc      540

aagcagctgc tgaaggtgca gaaggcctac caccagctgg atcagtcctt tatcgataca      600

tatatcgacc tgctggagac aaggcgcaca tactatgagg gaccaggaga gggctctccc      660

ttcggctgga aggacatcaa ggagtggtac gagatgctga tgggccactg cacctatttt      720

ccagaggagc tgagaagcgt gaagtacgcc tataacgccg atctgtacaa cgccctgaat      780

gacctgaaca acctggtcat caccagggat gagaacgaga agctggagta ctatgagaag      840

ttccagatca tcgagaacgt gttcaagcag aagaagaagc ctacactgaa gcagatcgcc      900

aaggagatcc tggtgaacga ggaggacatc aagggctacc gcgtgacctc cacaggcaag      960

ccagagttca ccaatctgaa ggtgtatcac gatatcaagg acatcacagc ccggaaggag     1020

atcatcgaga acgccgagct gctggatcag atcgccaaga tcctgaccat ctatcagagc     1080

tccgaggaca tccaggagga gctgaccaac ctgaatagcg agctgacaca ggaggagatc     1140

gagcagatca gcaatctgaa gggctacacc ggcacacaca acctgagcct gaaggccatc     1200

aatctgatcc tggatgagct gtggcacaca aacgacaatc agatcgccat ctttaaccgg     1260

ctgaagctgg tgccaaagaa ggtggacctg tcccagcaga aggagatccc aaccacactg     1320

gtggacgatt tcatcctgtc tcccgtggtg aagcggagct tcatccagag catcaaagtg     1380

atcaacgcca tcatcaagaa gtacggcctg cccaatgata tcatcatcga gctggccagg     1440

gagaagaact ccaaggacgc ccagaagatg atcaatgaga tgcagaagag gaaccgccag     1500

accaatgagc ggatcgagga gatcatcaga accacaggca aggagaacgc caagtacctg     1560

atcgagaaga tcaagctgca cgatatgcag gagggcaagt gtctgtattc tctggaggcc     1620

atccctctgg aggacctgct gaacaatcca ttcaactacg aggtggatca catcatcccc     1680

cggagcgtga gcttcgacaa ttcttttaac aataaggtgc tggtgaagca ggaggagaac     1740

agcaagaagg gcaataggac ccctttccag tacctgtcta gctccgattc taagatcagc     1800

tacgagacat tcaagaagca catcctgaat ctggccaagg gcaagggccg catcagcaag     1860

accaagaagg agtacctgct ggaggagcgg gacatcaaca gattctccgt gcagaaggac     1920

ttcatcaacc ggaatctggt ggacaccaga tacgccacac gcggcctgat gaatctgctg     1980

cggtcttatt tcagagtgaa caatctggat gtgaaggtga agagcatcaa cggcggcttc     2040

acctcctttc tgcggagaaa gtggaagttt aagaaggagc gcaacaaggg ctataagcac     2100

cacgccgagg atgccctgat catcgccaat gccgacttca tctttaagga gtggaagaag     2160

ctggacaagg ccaagaaagt gatggagaac cagatgttcg aggagaagca ggccgagagc     2220

atgcccccga agaagaaacg taaggtgggt agcagcggta gcccgaaaaa gaagcgcaaa     2280

gtgggcagcg acgcgctgga cgatttcgat ctggacatgc tgggcagcga tgcgctggat     2340

gatttcggcg gtggcagctc tgaggtggag ttttcccacg agtactggat gagacatgcc     2400

ctgaccctgg ccaagagggc acgcgatgag agggaggtgc ctgtgggagc cgtgctggtg     2460

ctgaacaata gagtgatcgg cgagggctgg aacagagcca tcggcctgca cgacccaaca     2520

gcccatgccg aaattatggc cctgagacag ggcggcctgg tcatgcagaa ctacagactg     2580

attgacgcca ccctgtacgg cacattcgag ccttgcgtga tgtgcgccgg cgccatgatc     2640

cactctagga tcggccgcgt ggtgtttggc gtgaggaacg caaaaaccgg cgccgcaggc     2700

tccctgatgg acgtgctgca ctaccccggc atgaatcacc gcgtcgaaat taccgaggga     2760

atcctggcag atgaatgtgc cgccctgctg tgctatttct ttcggatgcc tagacaggtg     2820

ttcaatgctc agaagaaggc ccagagctcc accgacggcg gtagcggcgg tagcggtggt     2880

agcggcggca gcggtggtag cggtggcagc ggtggtcccg agatcgagac agagcaggag     2940

tacaaggaga ttttcatcac acctcaccag atcaagcaca tcaaggactt caaggactac     3000

aagtattctc acagggtgga taagaagccc aaccgcgagc tgatcaatga caccctgtat     3060

agcacacgga aggacgataa gggcaatacc ctgatcgtga acaatctgaa cggcctgtac     3120

gacaaggata atgacaagct gaagaagctg atcaacaagt ctcccgagaa gctgctgatg     3180

taccaccacg atcctcagac atatcagaag ctgaagctga tcatggagca gtacggcgac     3240

gagaagaacc cactgtataa gtactatgag gagacaggca actacctgac aaagtatagc     3300

aagaaggata atggccccgt gatcaagaag atcaagtact atggcaacaa gctgaatgcc     3360

cacctggaca tcaccgacga ttaccctaac tctcgcaata aggtggtgaa gctgagcctg     3420

aagccatacc ggttcgacgt gtacctggac aacggcgtgt ataagtttgt gacagtgaag     3480

aatctggatg tgatcaagaa ggagaactac tatgaggtga acagcaagtg ctacgaggag     3540

gccaagaagc tgaagaagat cagcaaccag gccgagttca tcgcctcttt ttacaacaat     3600

gacctgatca agatcaatgg cgagctgtat agagtgatcg gcgtgaacaa tgatctgctg     3660

aacagaatcg aagtgaatat gatcgacatc acctacaggg agtatctgga gaacatgaat     3720

gataagaggc cccctcgcat catcaagacc atcgcctcta agacacagag catcaagaag     3780

tacagcacag acatcctggg gaacctgtat gaagtcaaga gcaagaaaca tcctcagatt     3840

atcaagaaag gctctggtgg ttctaagcga cctgccgcca caaagaaggc tggacaggct     3900

aagaagaaga aagattacaa agacgatgac gataagggat ccggcgcaac aaacttctct     3960

ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgatggccaa gcctttgtct     4020

caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct     4080

gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc     4140

aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct     4200

gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc     4260

ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc     4320

atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct     4380

ggttatgtgt gggagggcta a                                               4401


<210>  145
<211>  4401
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nSaCas9 Q731 miniABEMax V82G

<400>  145
atgaagcgaa attacattct ggggctggcc attggcatta catcagtggg ctatggcatc       60

attgactacg agacaaggga cgtgatcgac gccggcgtga gactgttcaa ggaggccaac      120

gtggagaaca atgagggccg gagatccaag aggggagcaa ggcgcctgaa gcggagaagg      180

cgccacagaa tccagagagt gaagaagctg ctgttcgatt acaacctgct gaccgaccac      240

tccgagctgt ctggcatcaa tccttatgag gccagagtga agggcctgtc ccagaagctg      300

tctgaggagg agtttagcgc cgccctgctg cacctggcaa agaggagagg cgtgcacaac      360

gtgaatgagg tggaggagga caccggcaac gagctgtcca caaaggagca gatcagccgc      420

aattccaagg ccctggagga gaagtatgtg gccgagctgc agctggagcg gctgaagaag      480

gatggcgagg tgaggggctc catcaatcgc ttcaagacct ctgactacgt gaaggaggcc      540

aagcagctgc tgaaggtgca gaaggcctac caccagctgg atcagtcctt tatcgataca      600

tatatcgacc tgctggagac aaggcgcaca tactatgagg gaccaggaga gggctctccc      660

ttcggctgga aggacatcaa ggagtggtac gagatgctga tgggccactg cacctatttt      720

ccagaggagc tgagaagcgt gaagtacgcc tataacgccg atctgtacaa cgccctgaat      780

gacctgaaca acctggtcat caccagggat gagaacgaga agctggagta ctatgagaag      840

ttccagatca tcgagaacgt gttcaagcag aagaagaagc ctacactgaa gcagatcgcc      900

aaggagatcc tggtgaacga ggaggacatc aagggctacc gcgtgacctc cacaggcaag      960

ccagagttca ccaatctgaa ggtgtatcac gatatcaagg acatcacagc ccggaaggag     1020

atcatcgaga acgccgagct gctggatcag atcgccaaga tcctgaccat ctatcagagc     1080

tccgaggaca tccaggagga gctgaccaac ctgaatagcg agctgacaca ggaggagatc     1140

gagcagatca gcaatctgaa gggctacacc ggcacacaca acctgagcct gaaggccatc     1200

aatctgatcc tggatgagct gtggcacaca aacgacaatc agatcgccat ctttaaccgg     1260

ctgaagctgg tgccaaagaa ggtggacctg tcccagcaga aggagatccc aaccacactg     1320

gtggacgatt tcatcctgtc tcccgtggtg aagcggagct tcatccagag catcaaagtg     1380

atcaacgcca tcatcaagaa gtacggcctg cccaatgata tcatcatcga gctggccagg     1440

gagaagaact ccaaggacgc ccagaagatg atcaatgaga tgcagaagag gaaccgccag     1500

accaatgagc ggatcgagga gatcatcaga accacaggca aggagaacgc caagtacctg     1560

atcgagaaga tcaagctgca cgatatgcag gagggcaagt gtctgtattc tctggaggcc     1620

atccctctgg aggacctgct gaacaatcca ttcaactacg aggtggatca catcatcccc     1680

cggagcgtga gcttcgacaa ttcttttaac aataaggtgc tggtgaagca ggaggagaac     1740

agcaagaagg gcaataggac ccctttccag tacctgtcta gctccgattc taagatcagc     1800

tacgagacat tcaagaagca catcctgaat ctggccaagg gcaagggccg catcagcaag     1860

accaagaagg agtacctgct ggaggagcgg gacatcaaca gattctccgt gcagaaggac     1920

ttcatcaacc ggaatctggt ggacaccaga tacgccacac gcggcctgat gaatctgctg     1980

cggtcttatt tcagagtgaa caatctggat gtgaaggtga agagcatcaa cggcggcttc     2040

acctcctttc tgcggagaaa gtggaagttt aagaaggagc gcaacaaggg ctataagcac     2100

cacgccgagg atgccctgat catcgccaat gccgacttca tctttaagga gtggaagaag     2160

ctggacaagg ccaagaaagt gatggagaac cagccgaaga agaaacgtaa ggtgggtagc     2220

agcggtagcc cgaaaaagaa gcgcaaagtg ggcagcgacg cgctggacga tttcgatctg     2280

gacatgctgg gcagcgatgc gctggatgat ttcggcggtg gcagctctga ggtggagttt     2340

tcccacgagt actggatgag acatgccctg accctggcca agagggcacg cgatgagagg     2400

gaggtgcctg tgggagccgt gctggtgctg aacaatagag tgatcggcga gggctggaac     2460

agagccatcg gcctgcacga cccaacagcc catgccgaaa ttatggccct gagacagggc     2520

ggcctggtca tgcagaacta cagactgatt gacgccaccc tgtacggcac attcgagcct     2580

tgcgtgatgt gcgccggcgc catgatccac tctaggatcg gccgcgtggt gtttggcgtg     2640

aggaacgcaa aaaccggcgc cgcaggctcc ctgatggacg tgctgcacta ccccggcatg     2700

aatcaccgcg tcgaaattac cgagggaatc ctggcagatg aatgtgccgc cctgctgtgc     2760

tatttctttc ggatgcctag acaggtgttc aatgctcaga agaaggccca gagctccacc     2820

gacggcggta gcggcggtag cggtggtagc ggcggcagcg gtggtagcgg tggcagcggt     2880

ggtcagatgt tcgaggagaa gcaggccgag agcatgcccg agatcgagac agagcaggag     2940

tacaaggaga ttttcatcac acctcaccag atcaagcaca tcaaggactt caaggactac     3000

aagtattctc acagggtgga taagaagccc aaccgcgagc tgatcaatga caccctgtat     3060

agcacacgga aggacgataa gggcaatacc ctgatcgtga acaatctgaa cggcctgtac     3120

gacaaggata atgacaagct gaagaagctg atcaacaagt ctcccgagaa gctgctgatg     3180

taccaccacg atcctcagac atatcagaag ctgaagctga tcatggagca gtacggcgac     3240

gagaagaacc cactgtataa gtactatgag gagacaggca actacctgac aaagtatagc     3300

aagaaggata atggccccgt gatcaagaag atcaagtact atggcaacaa gctgaatgcc     3360

cacctggaca tcaccgacga ttaccctaac tctcgcaata aggtggtgaa gctgagcctg     3420

aagccatacc ggttcgacgt gtacctggac aacggcgtgt ataagtttgt gacagtgaag     3480

aatctggatg tgatcaagaa ggagaactac tatgaggtga acagcaagtg ctacgaggag     3540

gccaagaagc tgaagaagat cagcaaccag gccgagttca tcgcctcttt ttacaacaat     3600

gacctgatca agatcaatgg cgagctgtat agagtgatcg gcgtgaacaa tgatctgctg     3660

aacagaatcg aagtgaatat gatcgacatc acctacaggg agtatctgga gaacatgaat     3720

gataagaggc cccctcgcat catcaagacc atcgcctcta agacacagag catcaagaag     3780

tacagcacag acatcctggg gaacctgtat gaagtcaaga gcaagaaaca tcctcagatt     3840

atcaagaaag gctctggtgg ttctaagcga cctgccgcca caaagaaggc tggacaggct     3900

aagaagaaga aagattacaa agacgatgac gataagggat ccggcgcaac aaacttctct     3960

ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgatggccaa gcctttgtct     4020

caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct     4080

gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc     4140

aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct     4200

gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc     4260

ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc     4320

atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct     4380

ggttatgtgt gggagggcta a                                               4401


<210>  146
<211>  4401
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nSaCas9 Q737 miniABEMax V82G

<400>  146
atgaagcgaa attacattct ggggctggcc attggcatta catcagtggg ctatggcatc       60

attgactacg agacaaggga cgtgatcgac gccggcgtga gactgttcaa ggaggccaac      120

gtggagaaca atgagggccg gagatccaag aggggagcaa ggcgcctgaa gcggagaagg      180

cgccacagaa tccagagagt gaagaagctg ctgttcgatt acaacctgct gaccgaccac      240

tccgagctgt ctggcatcaa tccttatgag gccagagtga agggcctgtc ccagaagctg      300

tctgaggagg agtttagcgc cgccctgctg cacctggcaa agaggagagg cgtgcacaac      360

gtgaatgagg tggaggagga caccggcaac gagctgtcca caaaggagca gatcagccgc      420

aattccaagg ccctggagga gaagtatgtg gccgagctgc agctggagcg gctgaagaag      480

gatggcgagg tgaggggctc catcaatcgc ttcaagacct ctgactacgt gaaggaggcc      540

aagcagctgc tgaaggtgca gaaggcctac caccagctgg atcagtcctt tatcgataca      600

tatatcgacc tgctggagac aaggcgcaca tactatgagg gaccaggaga gggctctccc      660

ttcggctgga aggacatcaa ggagtggtac gagatgctga tgggccactg cacctatttt      720

ccagaggagc tgagaagcgt gaagtacgcc tataacgccg atctgtacaa cgccctgaat      780

gacctgaaca acctggtcat caccagggat gagaacgaga agctggagta ctatgagaag      840

ttccagatca tcgagaacgt gttcaagcag aagaagaagc ctacactgaa gcagatcgcc      900

aaggagatcc tggtgaacga ggaggacatc aagggctacc gcgtgacctc cacaggcaag      960

ccagagttca ccaatctgaa ggtgtatcac gatatcaagg acatcacagc ccggaaggag     1020

atcatcgaga acgccgagct gctggatcag atcgccaaga tcctgaccat ctatcagagc     1080

tccgaggaca tccaggagga gctgaccaac ctgaatagcg agctgacaca ggaggagatc     1140

gagcagatca gcaatctgaa gggctacacc ggcacacaca acctgagcct gaaggccatc     1200

aatctgatcc tggatgagct gtggcacaca aacgacaatc agatcgccat ctttaaccgg     1260

ctgaagctgg tgccaaagaa ggtggacctg tcccagcaga aggagatccc aaccacactg     1320

gtggacgatt tcatcctgtc tcccgtggtg aagcggagct tcatccagag catcaaagtg     1380

atcaacgcca tcatcaagaa gtacggcctg cccaatgata tcatcatcga gctggccagg     1440

gagaagaact ccaaggacgc ccagaagatg atcaatgaga tgcagaagag gaaccgccag     1500

accaatgagc ggatcgagga gatcatcaga accacaggca aggagaacgc caagtacctg     1560

atcgagaaga tcaagctgca cgatatgcag gagggcaagt gtctgtattc tctggaggcc     1620

atccctctgg aggacctgct gaacaatcca ttcaactacg aggtggatca catcatcccc     1680

cggagcgtga gcttcgacaa ttcttttaac aataaggtgc tggtgaagca ggaggagaac     1740

agcaagaagg gcaataggac ccctttccag tacctgtcta gctccgattc taagatcagc     1800

tacgagacat tcaagaagca catcctgaat ctggccaagg gcaagggccg catcagcaag     1860

accaagaagg agtacctgct ggaggagcgg gacatcaaca gattctccgt gcagaaggac     1920

ttcatcaacc ggaatctggt ggacaccaga tacgccacac gcggcctgat gaatctgctg     1980

cggtcttatt tcagagtgaa caatctggat gtgaaggtga agagcatcaa cggcggcttc     2040

acctcctttc tgcggagaaa gtggaagttt aagaaggagc gcaacaaggg ctataagcac     2100

cacgccgagg atgccctgat catcgccaat gccgacttca tctttaagga gtggaagaag     2160

ctggacaagg ccaagaaagt gatggagaac cagatgttcg aggagaagca gccgaagaag     2220

aaacgtaagg tgggtagcag cggtagcccg aaaaagaagc gcaaagtggg cagcgacgcg     2280

ctggacgatt tcgatctgga catgctgggc agcgatgcgc tggatgattt cggcggtggc     2340

agctctgagg tggagttttc ccacgagtac tggatgagac atgccctgac cctggccaag     2400

agggcacgcg atgagaggga ggtgcctgtg ggagccgtgc tggtgctgaa caatagagtg     2460

atcggcgagg gctggaacag agccatcggc ctgcacgacc caacagccca tgccgaaatt     2520

atggccctga gacagggcgg cctggtcatg cagaactaca gactgattga cgccaccctg     2580

tacggcacat tcgagccttg cgtgatgtgc gccggcgcca tgatccactc taggatcggc     2640

cgcgtggtgt ttggcgtgag gaacgcaaaa accggcgccg caggctccct gatggacgtg     2700

ctgcactacc ccggcatgaa tcaccgcgtc gaaattaccg agggaatcct ggcagatgaa     2760

tgtgccgccc tgctgtgcta tttctttcgg atgcctagac aggtgttcaa tgctcagaag     2820

aaggcccaga gctccaccga cggcggtagc ggcggtagcg gtggtagcgg cggcagcggt     2880

ggtagcggtg gcagcggtgg tcaggccgag agcatgcccg agatcgagac agagcaggag     2940

tacaaggaga ttttcatcac acctcaccag atcaagcaca tcaaggactt caaggactac     3000

aagtattctc acagggtgga taagaagccc aaccgcgagc tgatcaatga caccctgtat     3060

agcacacgga aggacgataa gggcaatacc ctgatcgtga acaatctgaa cggcctgtac     3120

gacaaggata atgacaagct gaagaagctg atcaacaagt ctcccgagaa gctgctgatg     3180

taccaccacg atcctcagac atatcagaag ctgaagctga tcatggagca gtacggcgac     3240

gagaagaacc cactgtataa gtactatgag gagacaggca actacctgac aaagtatagc     3300

aagaaggata atggccccgt gatcaagaag atcaagtact atggcaacaa gctgaatgcc     3360

cacctggaca tcaccgacga ttaccctaac tctcgcaata aggtggtgaa gctgagcctg     3420

aagccatacc ggttcgacgt gtacctggac aacggcgtgt ataagtttgt gacagtgaag     3480

aatctggatg tgatcaagaa ggagaactac tatgaggtga acagcaagtg ctacgaggag     3540

gccaagaagc tgaagaagat cagcaaccag gccgagttca tcgcctcttt ttacaacaat     3600

gacctgatca agatcaatgg cgagctgtat agagtgatcg gcgtgaacaa tgatctgctg     3660

aacagaatcg aagtgaatat gatcgacatc acctacaggg agtatctgga gaacatgaat     3720

gataagaggc cccctcgcat catcaagacc atcgcctcta agacacagag catcaagaag     3780

tacagcacag acatcctggg gaacctgtat gaagtcaaga gcaagaaaca tcctcagatt     3840

atcaagaaag gctctggtgg ttctaagcga cctgccgcca caaagaaggc tggacaggct     3900

aagaagaaga aagattacaa agacgatgac gataagggat ccggcgcaac aaacttctct     3960

ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgatggccaa gcctttgtct     4020

caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct     4080

gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc     4140

aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct     4200

gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc     4260

ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc     4320

atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct     4380

ggttatgtgt gggagggcta a                                               4401


<210>  147
<211>  4401
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nSaCas9 S740 miniABEMax V82G

<400>  147
atgaagcgaa attacattct ggggctggcc attggcatta catcagtggg ctatggcatc       60

attgactacg agacaaggga cgtgatcgac gccggcgtga gactgttcaa ggaggccaac      120

gtggagaaca atgagggccg gagatccaag aggggagcaa ggcgcctgaa gcggagaagg      180

cgccacagaa tccagagagt gaagaagctg ctgttcgatt acaacctgct gaccgaccac      240

tccgagctgt ctggcatcaa tccttatgag gccagagtga agggcctgtc ccagaagctg      300

tctgaggagg agtttagcgc cgccctgctg cacctggcaa agaggagagg cgtgcacaac      360

gtgaatgagg tggaggagga caccggcaac gagctgtcca caaaggagca gatcagccgc      420

aattccaagg ccctggagga gaagtatgtg gccgagctgc agctggagcg gctgaagaag      480

gatggcgagg tgaggggctc catcaatcgc ttcaagacct ctgactacgt gaaggaggcc      540

aagcagctgc tgaaggtgca gaaggcctac caccagctgg atcagtcctt tatcgataca      600

tatatcgacc tgctggagac aaggcgcaca tactatgagg gaccaggaga gggctctccc      660

ttcggctgga aggacatcaa ggagtggtac gagatgctga tgggccactg cacctatttt      720

ccagaggagc tgagaagcgt gaagtacgcc tataacgccg atctgtacaa cgccctgaat      780

gacctgaaca acctggtcat caccagggat gagaacgaga agctggagta ctatgagaag      840

ttccagatca tcgagaacgt gttcaagcag aagaagaagc ctacactgaa gcagatcgcc      900

aaggagatcc tggtgaacga ggaggacatc aagggctacc gcgtgacctc cacaggcaag      960

ccagagttca ccaatctgaa ggtgtatcac gatatcaagg acatcacagc ccggaaggag     1020

atcatcgaga acgccgagct gctggatcag atcgccaaga tcctgaccat ctatcagagc     1080

tccgaggaca tccaggagga gctgaccaac ctgaatagcg agctgacaca ggaggagatc     1140

gagcagatca gcaatctgaa gggctacacc ggcacacaca acctgagcct gaaggccatc     1200

aatctgatcc tggatgagct gtggcacaca aacgacaatc agatcgccat ctttaaccgg     1260

ctgaagctgg tgccaaagaa ggtggacctg tcccagcaga aggagatccc aaccacactg     1320

gtggacgatt tcatcctgtc tcccgtggtg aagcggagct tcatccagag catcaaagtg     1380

atcaacgcca tcatcaagaa gtacggcctg cccaatgata tcatcatcga gctggccagg     1440

gagaagaact ccaaggacgc ccagaagatg atcaatgaga tgcagaagag gaaccgccag     1500

accaatgagc ggatcgagga gatcatcaga accacaggca aggagaacgc caagtacctg     1560

atcgagaaga tcaagctgca cgatatgcag gagggcaagt gtctgtattc tctggaggcc     1620

atccctctgg aggacctgct gaacaatcca ttcaactacg aggtggatca catcatcccc     1680

cggagcgtga gcttcgacaa ttcttttaac aataaggtgc tggtgaagca ggaggagaac     1740

agcaagaagg gcaataggac ccctttccag tacctgtcta gctccgattc taagatcagc     1800

tacgagacat tcaagaagca catcctgaat ctggccaagg gcaagggccg catcagcaag     1860

accaagaagg agtacctgct ggaggagcgg gacatcaaca gattctccgt gcagaaggac     1920

ttcatcaacc ggaatctggt ggacaccaga tacgccacac gcggcctgat gaatctgctg     1980

cggtcttatt tcagagtgaa caatctggat gtgaaggtga agagcatcaa cggcggcttc     2040

acctcctttc tgcggagaaa gtggaagttt aagaaggagc gcaacaaggg ctataagcac     2100

cacgccgagg atgccctgat catcgccaat gccgacttca tctttaagga gtggaagaag     2160

ctggacaagg ccaagaaagt gatggagaac cagatgttcg aggagaagca ggccgagagc     2220

ccgaagaaga aacgtaaggt gggtagcagc ggtagcccga aaaagaagcg caaagtgggc     2280

agcgacgcgc tggacgattt cgatctggac atgctgggca gcgatgcgct ggatgatttc     2340

ggcggtggca gctctgaggt ggagttttcc cacgagtact ggatgagaca tgccctgacc     2400

ctggccaaga gggcacgcga tgagagggag gtgcctgtgg gagccgtgct ggtgctgaac     2460

aatagagtga tcggcgaggg ctggaacaga gccatcggcc tgcacgaccc aacagcccat     2520

gccgaaatta tggccctgag acagggcggc ctggtcatgc agaactacag actgattgac     2580

gccaccctgt acggcacatt cgagccttgc gtgatgtgcg ccggcgccat gatccactct     2640

aggatcggcc gcgtggtgtt tggcgtgagg aacgcaaaaa ccggcgccgc aggctccctg     2700

atggacgtgc tgcactaccc cggcatgaat caccgcgtcg aaattaccga gggaatcctg     2760

gcagatgaat gtgccgccct gctgtgctat ttctttcgga tgcctagaca ggtgttcaat     2820

gctcagaaga aggcccagag ctccaccgac ggcggtagcg gcggtagcgg tggtagcggc     2880

ggcagcggtg gtagcggtgg cagcggtggt agcatgcccg agatcgagac agagcaggag     2940

tacaaggaga ttttcatcac acctcaccag atcaagcaca tcaaggactt caaggactac     3000

aagtattctc acagggtgga taagaagccc aaccgcgagc tgatcaatga caccctgtat     3060

agcacacgga aggacgataa gggcaatacc ctgatcgtga acaatctgaa cggcctgtac     3120

gacaaggata atgacaagct gaagaagctg atcaacaagt ctcccgagaa gctgctgatg     3180

taccaccacg atcctcagac atatcagaag ctgaagctga tcatggagca gtacggcgac     3240

gagaagaacc cactgtataa gtactatgag gagacaggca actacctgac aaagtatagc     3300

aagaaggata atggccccgt gatcaagaag atcaagtact atggcaacaa gctgaatgcc     3360

cacctggaca tcaccgacga ttaccctaac tctcgcaata aggtggtgaa gctgagcctg     3420

aagccatacc ggttcgacgt gtacctggac aacggcgtgt ataagtttgt gacagtgaag     3480

aatctggatg tgatcaagaa ggagaactac tatgaggtga acagcaagtg ctacgaggag     3540

gccaagaagc tgaagaagat cagcaaccag gccgagttca tcgcctcttt ttacaacaat     3600

gacctgatca agatcaatgg cgagctgtat agagtgatcg gcgtgaacaa tgatctgctg     3660

aacagaatcg aagtgaatat gatcgacatc acctacaggg agtatctgga gaacatgaat     3720

gataagaggc cccctcgcat catcaagacc atcgcctcta agacacagag catcaagaag     3780

tacagcacag acatcctggg gaacctgtat gaagtcaaga gcaagaaaca tcctcagatt     3840

atcaagaaag gctctggtgg ttctaagcga cctgccgcca caaagaaggc tggacaggct     3900

aagaagaaga aagattacaa agacgatgac gataagggat ccggcgcaac aaacttctct     3960

ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgatggccaa gcctttgtct     4020

caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct     4080

gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc     4140

aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct     4200

gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc     4260

ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc     4320

atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct     4380

ggttatgtgt gggagggcta a                                               4401


<210>  148
<211>  4401
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nSaCas9 T128 miniABEMax V82G

<400>  148
atgaagcgaa attacattct ggggctggcc attggcatta catcagtggg ctatggcatc       60

attgactacg agacaaggga cgtgatcgac gccggcgtga gactgttcaa ggaggccaac      120

gtggagaaca atgagggccg gagatccaag aggggagcaa ggcgcctgaa gcggagaagg      180

cgccacagaa tccagagagt gaagaagctg ctgttcgatt acaacctgct gaccgaccac      240

tccgagctgt ctggcatcaa tccttatgag gccagagtga agggcctgtc ccagaagctg      300

tctgaggagg agtttagcgc cgccctgctg cacctggcaa agaggagagg cgtgcacaac      360

gtgaatgagg tggaggagga caccccgaag aagaaacgta aggtgggtag cagcggtagc      420

ccgaaaaaga agcgcaaagt gggcagcgac gcgctggacg atttcgatct ggacatgctg      480

ggcagcgatg cgctggatga tttcggcggt ggcagctctg aggtggagtt ttcccacgag      540

tactggatga gacatgccct gaccctggcc aagagggcac gcgatgagag ggaggtgcct      600

gtgggagccg tgctggtgct gaacaataga gtgatcggcg agggctggaa cagagccatc      660

ggcctgcacg acccaacagc ccatgccgaa attatggccc tgagacaggg cggcctggtc      720

atgcagaact acagactgat tgacgccacc ctgtacggca cattcgagcc ttgcgtgatg      780

tgcgccggcg ccatgatcca ctctaggatc ggccgcgtgg tgtttggcgt gaggaacgca      840

aaaaccggcg ccgcaggctc cctgatggac gtgctgcact accccggcat gaatcaccgc      900

gtcgaaatta ccgagggaat cctggcagat gaatgtgccg ccctgctgtg ctatttcttt      960

cggatgccta gacaggtgtt caatgctcag aagaaggccc agagctccac cgacggcggt     1020

agcggcggta gcggtggtag cggcggcagc ggtggtagcg gtggcagcgg tggtaccggc     1080

aacgagctgt ccacaaagga gcagatcagc cgcaattcca aggccctgga ggagaagtat     1140

gtggccgagc tgcagctgga gcggctgaag aaggatggcg aggtgagggg ctccatcaat     1200

cgcttcaaga cctctgacta cgtgaaggag gccaagcagc tgctgaaggt gcagaaggcc     1260

taccaccagc tggatcagtc ctttatcgat acatatatcg acctgctgga gacaaggcgc     1320

acatactatg agggaccagg agagggctct cccttcggct ggaaggacat caaggagtgg     1380

tacgagatgc tgatgggcca ctgcacctat tttccagagg agctgagaag cgtgaagtac     1440

gcctataacg ccgatctgta caacgccctg aatgacctga acaacctggt catcaccagg     1500

gatgagaacg agaagctgga gtactatgag aagttccaga tcatcgagaa cgtgttcaag     1560

cagaagaaga agcctacact gaagcagatc gccaaggaga tcctggtgaa cgaggaggac     1620

atcaagggct accgcgtgac ctccacaggc aagccagagt tcaccaatct gaaggtgtat     1680

cacgatatca aggacatcac agcccggaag gagatcatcg agaacgccga gctgctggat     1740

cagatcgcca agatcctgac catctatcag agctccgagg acatccagga ggagctgacc     1800

aacctgaata gcgagctgac acaggaggag atcgagcaga tcagcaatct gaagggctac     1860

accggcacac acaacctgag cctgaaggcc atcaatctga tcctggatga gctgtggcac     1920

acaaacgaca atcagatcgc catctttaac cggctgaagc tggtgccaaa gaaggtggac     1980

ctgtcccagc agaaggagat cccaaccaca ctggtggacg atttcatcct gtctcccgtg     2040

gtgaagcgga gcttcatcca gagcatcaaa gtgatcaacg ccatcatcaa gaagtacggc     2100

ctgcccaatg atatcatcat cgagctggcc agggagaaga actccaagga cgcccagaag     2160

atgatcaatg agatgcagaa gaggaaccgc cagaccaatg agcggatcga ggagatcatc     2220

agaaccacag gcaaggagaa cgccaagtac ctgatcgaga agatcaagct gcacgatatg     2280

caggagggca agtgtctgta ttctctggag gccatccctc tggaggacct gctgaacaat     2340

ccattcaact acgaggtgga tcacatcatc ccccggagcg tgagcttcga caattctttt     2400

aacaataagg tgctggtgaa gcaggaggag aacagcaaga agggcaatag gacccctttc     2460

cagtacctgt ctagctccga ttctaagatc agctacgaga cattcaagaa gcacatcctg     2520

aatctggcca agggcaaggg ccgcatcagc aagaccaaga aggagtacct gctggaggag     2580

cgggacatca acagattctc cgtgcagaag gacttcatca accggaatct ggtggacacc     2640

agatacgcca cacgcggcct gatgaatctg ctgcggtctt atttcagagt gaacaatctg     2700

gatgtgaagg tgaagagcat caacggcggc ttcacctcct ttctgcggag aaagtggaag     2760

tttaagaagg agcgcaacaa gggctataag caccacgccg aggatgccct gatcatcgcc     2820

aatgccgact tcatctttaa ggagtggaag aagctggaca aggccaagaa agtgatggag     2880

aaccagatgt tcgaggagaa gcaggccgag agcatgcccg agatcgagac agagcaggag     2940

tacaaggaga ttttcatcac acctcaccag atcaagcaca tcaaggactt caaggactac     3000

aagtattctc acagggtgga taagaagccc aaccgcgagc tgatcaatga caccctgtat     3060

agcacacgga aggacgataa gggcaatacc ctgatcgtga acaatctgaa cggcctgtac     3120

gacaaggata atgacaagct gaagaagctg atcaacaagt ctcccgagaa gctgctgatg     3180

taccaccacg atcctcagac atatcagaag ctgaagctga tcatggagca gtacggcgac     3240

gagaagaacc cactgtataa gtactatgag gagacaggca actacctgac aaagtatagc     3300

aagaaggata atggccccgt gatcaagaag atcaagtact atggcaacaa gctgaatgcc     3360

cacctggaca tcaccgacga ttaccctaac tctcgcaata aggtggtgaa gctgagcctg     3420

aagccatacc ggttcgacgt gtacctggac aacggcgtgt ataagtttgt gacagtgaag     3480

aatctggatg tgatcaagaa ggagaactac tatgaggtga acagcaagtg ctacgaggag     3540

gccaagaagc tgaagaagat cagcaaccag gccgagttca tcgcctcttt ttacaacaat     3600

gacctgatca agatcaatgg cgagctgtat agagtgatcg gcgtgaacaa tgatctgctg     3660

aacagaatcg aagtgaatat gatcgacatc acctacaggg agtatctgga gaacatgaat     3720

gataagaggc cccctcgcat catcaagacc atcgcctcta agacacagag catcaagaag     3780

tacagcacag acatcctggg gaacctgtat gaagtcaaga gcaagaaaca tcctcagatt     3840

atcaagaaag gctctggtgg ttctaagcga cctgccgcca caaagaaggc tggacaggct     3900

aagaagaaga aagattacaa agacgatgac gataagggat ccggcgcaac aaacttctct     3960

ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgatggccaa gcctttgtct     4020

caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct     4080

gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc     4140

aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct     4200

gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc     4260

ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc     4320

atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct     4380

ggttatgtgt gggagggcta a                                               4401


<210>  149
<211>  4722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nSaCas9 G129 AIDx

<400>  149
gctagcgcca ccatgaagcg aaattacatt ctggggctgg ccattggcat tacatcagtg       60

ggctatggca tcattgacta cgagacaagg gacgtgatcg acgccggcgt gagactgttc      120

aaggaggcca acgtggagaa caatgagggc cggagatcca agaggggagc aaggcgcctg      180

aagcggagaa ggcgccacag aatccagaga gtgaagaagc tgctgttcga ttacaacctg      240

ctgaccgacc actccgagct gtctggcatc aatccttatg aggccagagt gaagggcctg      300

tcccagaagc tgtctgagga ggagtttagc gccgccctgc tgcacctggc aaagaggaga      360

ggcgtgcaca acgtgaatga ggtggaggag gacaccggcc cgaagaagaa acgtaaggtg      420

ggtagcagcg gtagcccgaa aaagaagcgc aaagtgggca gcgacgcgct ggacgatttc      480

gatctggaca tgctgggcag cgatgcgctg gatgatttcg gcggtggcag catggatagc      540

ctgctgatga accgtcgcaa gttcctgtac cagttcaaaa acgtgcgttg ggcgaagggt      600

cgtcgtgaga cctacctgtg ctacgtggtg aagcgtcgtg acagcgcgac cagcttcagc      660

ctggattttg gctatctgcg taacaaaaac ggttgccacg tggaactgct gtttctgcgc      720

tacattagcg actgggacct ggaccccggt cgctgctacc gcgtgacctg gtttaccagc      780

tggagcccgt gctatgattg cgcgcgtcac gtggccgatt tcctgcgcgg taacccgaac      840

ctgagcctgc gcattttcac cgcgcgcctg tatttctgcg aggatcgcaa ggcggagccg      900

gagggcctgc gtcgtctgca ccgtgctggt gtgcagattg cgattatgac ctttaaggac      960

tacttttatt gctggaacac ctttgtggag aaccacgagc gcaccttcaa agcgtgggag     1020

ggtctgcacg agaacagcgt gcgtctgagc cgccagctgc gtcgcatcct gctgccgggc     1080

ggtagcggcg gtagcggtgg tagcggcggc agcggtggta gcggtggcag cggtggtggc     1140

aacgagctgt ccacaaagga gcagatcagc cgcaattcca aggccctgga ggagaagtat     1200

gtggccgagc tgcagctgga gcggctgaag aaggatggcg aggtgagggg ctccatcaat     1260

cgcttcaaga cctctgacta cgtgaaggag gccaagcagc tgctgaaggt gcagaaggcc     1320

taccaccagc tggatcagtc ctttatcgat acatatatcg acctgctgga gacaaggcgc     1380

acatactatg agggaccagg agagggctct cccttcggct ggaaggacat caaggagtgg     1440

tacgagatgc tgatgggcca ctgcacctat tttccagagg agctgagaag cgtgaagtac     1500

gcctataacg ccgatctgta caacgccctg aatgacctga acaacctggt catcaccagg     1560

gatgagaacg agaagctgga gtactatgag aagttccaga tcatcgagaa cgtgttcaag     1620

cagaagaaga agcctacact gaagcagatc gccaaggaga tcctggtgaa cgaggaggac     1680

atcaagggct accgcgtgac ctccacaggc aagccagagt tcaccaatct gaaggtgtat     1740

cacgatatca aggacatcac agcccggaag gagatcatcg agaacgccga gctgctggat     1800

cagatcgcca agatcctgac catctatcag agctccgagg acatccagga ggagctgacc     1860

aacctgaata gcgagctgac acaggaggag atcgagcaga tcagcaatct gaagggctac     1920

accggcacac acaacctgag cctgaaggcc atcaatctga tcctggatga gctgtggcac     1980

acaaacgaca atcagatcgc catctttaac cggctgaagc tggtgccaaa gaaggtggac     2040

ctgtcccagc agaaggagat cccaaccaca ctggtggacg atttcatcct gtctcccgtg     2100

gtgaagcgga gcttcatcca gagcatcaaa gtgatcaacg ccatcatcaa gaagtacggc     2160

ctgcccaatg atatcatcat cgagctggcc agggagaaga actccaagga cgcccagaag     2220

atgatcaatg agatgcagaa gaggaaccgc cagaccaatg agcggatcga ggagatcatc     2280

agaaccacag gcaaggagaa cgccaagtac ctgatcgaga agatcaagct gcacgatatg     2340

caggagggca agtgtctgta ttctctggag gccatccctc tggaggacct gctgaacaat     2400

ccattcaact acgaggtgga tcacatcatc ccccggagcg tgagcttcga caattctttt     2460

aacaataagg tgctggtgaa gcaggaggag aacagcaaga agggcaatag gacccctttc     2520

cagtacctgt ctagctccga ttctaagatc agctacgaga cattcaagaa gcacatcctg     2580

aatctggcca agggcaaggg ccgcatcagc aagaccaaga aggagtacct gctggaggag     2640

cgggacatca acagattctc cgtgcagaag gacttcatca accggaatct ggtggacacc     2700

agatacgcca cacgcggcct gatgaatctg ctgcggtctt atttcagagt gaacaatctg     2760

gatgtgaagg tgaagagcat caacggcggc ttcacctcct ttctgcggag aaagtggaag     2820

tttaagaagg agcgcaacaa gggctataag caccacgccg aggatgccct gatcatcgcc     2880

aatgccgact tcatctttaa ggagtggaag aagctggaca aggccaagaa agtgatggag     2940

aaccagatgt tcgaggagaa gcaggccgag agcatgcccg agatcgagac agagcaggag     3000

tacaaggaga ttttcatcac acctcaccag atcaagcaca tcaaggactt caaggactac     3060

aagtattctc acagggtgga taagaagccc aaccgcgagc tgatcaatga caccctgtat     3120

agcacacgga aggacgataa gggcaatacc ctgatcgtga acaatctgaa cggcctgtac     3180

gacaaggata atgacaagct gaagaagctg atcaacaagt ctcccgagaa gctgctgatg     3240

taccaccacg atcctcagac atatcagaag ctgaagctga tcatggagca gtacggcgac     3300

gagaagaacc cactgtataa gtactatgag gagacaggca actacctgac aaagtatagc     3360

aagaaggata atggccccgt gatcaagaag atcaagtact atggcaacaa gctgaatgcc     3420

cacctggaca tcaccgacga ttaccctaac tctcgcaata aggtggtgaa gctgagcctg     3480

aagccatacc ggttcgacgt gtacctggac aacggcgtgt ataagtttgt gacagtgaag     3540

aatctggatg tgatcaagaa ggagaactac tatgaggtga acagcaagtg ctacgaggag     3600

gccaagaagc tgaagaagat cagcaaccag gccgagttca tcgcctcttt ttacaacaat     3660

gacctgatca agatcaatgg cgagctgtat agagtgatcg gcgtgaacaa tgatctgctg     3720

aacagaatcg aagtgaatat gatcgacatc acctacaggg agtatctgga gaacatgaat     3780

gataagaggc cccctcgcat catcaagacc atcgcctcta agacacagag catcaagaag     3840

tacagcacag acatcctggg gaacctgtat gaagtcaaga gcaagaaaca tcctcagatt     3900

atcaagaaag gctctggtgg ttctaccaac ctgagcgaca tcattgagaa agagaccggc     3960

aagcagctgg taatccagga aagcattctg atgctgccgg aagaagtgga ggaagtgatt     4020

ggtaacaaac cggaaagcga catcctggtg cacaccgctt atgacgagag caccgacgaa     4080

aacgtgatgc tgctgaccag cgacgcgccg gagtataagc cgtgggcgct ggtaattcag     4140

gatagcaacg gcgaaaacaa aatcaagatg ctgtctggtg gttctaagcg acctgccgcc     4200

acaaagaagg ctggacaggc taagaagaag aaagattaca aagacgatga cgataaggga     4260

tccggcgcaa caaacttctc tctgctgaaa caagccggag atgtcgaaga gaatcctgga     4320

ccgatggcca agcctttgtc tcaagaagaa tccaccctca ttgaaagagc aacggctaca     4380

atcaacagca tccccatctc tgaagactac agcgtcgcca gcgcagctct ctctagcgac     4440

ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg tgcagaactc     4500

gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc     4560

ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt gcttctcgat     4620

ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac ggcagttggg     4680

attcgtgaat tgctgccctc tggttatgtg tgggagggct aa                        4722


<210>  150
<211>  4722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nSaCas9 I744 AIDx

<400>  150
gctagcgcca ccatgaagcg aaattacatt ctggggctgg ccattggcat tacatcagtg       60

ggctatggca tcattgacta cgagacaagg gacgtgatcg acgccggcgt gagactgttc      120

aaggaggcca acgtggagaa caatgagggc cggagatcca agaggggagc aaggcgcctg      180

aagcggagaa ggcgccacag aatccagaga gtgaagaagc tgctgttcga ttacaacctg      240

ctgaccgacc actccgagct gtctggcatc aatccttatg aggccagagt gaagggcctg      300

tcccagaagc tgtctgagga ggagtttagc gccgccctgc tgcacctggc aaagaggaga      360

ggcgtgcaca acgtgaatga ggtggaggag gacaccggca acgagctgtc cacaaaggag      420

cagatcagcc gcaattccaa ggccctggag gagaagtatg tggccgagct gcagctggag      480

cggctgaaga aggatggcga ggtgaggggc tccatcaatc gcttcaagac ctctgactac      540

gtgaaggagg ccaagcagct gctgaaggtg cagaaggcct accaccagct ggatcagtcc      600

tttatcgata catatatcga cctgctggag acaaggcgca catactatga gggaccagga      660

gagggctctc ccttcggctg gaaggacatc aaggagtggt acgagatgct gatgggccac      720

tgcacctatt ttccagagga gctgagaagc gtgaagtacg cctataacgc cgatctgtac      780

aacgccctga atgacctgaa caacctggtc atcaccaggg atgagaacga gaagctggag      840

tactatgaga agttccagat catcgagaac gtgttcaagc agaagaagaa gcctacactg      900

aagcagatcg ccaaggagat cctggtgaac gaggaggaca tcaagggcta ccgcgtgacc      960

tccacaggca agccagagtt caccaatctg aaggtgtatc acgatatcaa ggacatcaca     1020

gcccggaagg agatcatcga gaacgccgag ctgctggatc agatcgccaa gatcctgacc     1080

atctatcaga gctccgagga catccaggag gagctgacca acctgaatag cgagctgaca     1140

caggaggaga tcgagcagat cagcaatctg aagggctaca ccggcacaca caacctgagc     1200

ctgaaggcca tcaatctgat cctggatgag ctgtggcaca caaacgacaa tcagatcgcc     1260

atctttaacc ggctgaagct ggtgccaaag aaggtggacc tgtcccagca gaaggagatc     1320

ccaaccacac tggtggacga tttcatcctg tctcccgtgg tgaagcggag cttcatccag     1380

agcatcaaag tgatcaacgc catcatcaag aagtacggcc tgcccaatga tatcatcatc     1440

gagctggcca gggagaagaa ctccaaggac gcccagaaga tgatcaatga gatgcagaag     1500

aggaaccgcc agaccaatga gcggatcgag gagatcatca gaaccacagg caaggagaac     1560

gccaagtacc tgatcgagaa gatcaagctg cacgatatgc aggagggcaa gtgtctgtat     1620

tctctggagg ccatccctct ggaggacctg ctgaacaatc cattcaacta cgaggtggat     1680

cacatcatcc cccggagcgt gagcttcgac aattctttta acaataaggt gctggtgaag     1740

caggaggaga acagcaagaa gggcaatagg acccctttcc agtacctgtc tagctccgat     1800

tctaagatca gctacgagac attcaagaag cacatcctga atctggccaa gggcaagggc     1860

cgcatcagca agaccaagaa ggagtacctg ctggaggagc gggacatcaa cagattctcc     1920

gtgcagaagg acttcatcaa ccggaatctg gtggacacca gatacgccac acgcggcctg     1980

atgaatctgc tgcggtctta tttcagagtg aacaatctgg atgtgaaggt gaagagcatc     2040

aacggcggct tcacctcctt tctgcggaga aagtggaagt ttaagaagga gcgcaacaag     2100

ggctataagc accacgccga ggatgccctg atcatcgcca atgccgactt catctttaag     2160

gagtggaaga agctggacaa ggccaagaaa gtgatggaga accagatgtt cgaggagaag     2220

caggccgaga gcatgcccga gatcccgaag aagaaacgta aggtgggtag cagcggtagc     2280

ccgaaaaaga agcgcaaagt gggcagcgac gcgctggacg atttcgatct ggacatgctg     2340

ggcagcgatg cgctggatga tttcggcggt ggcagcatgg atagcctgct gatgaaccgt     2400

cgcaagttcc tgtaccagtt caaaaacgtg cgttgggcga agggtcgtcg tgagacctac     2460

ctgtgctacg tggtgaagcg tcgtgacagc gcgaccagct tcagcctgga ttttggctat     2520

ctgcgtaaca aaaacggttg ccacgtggaa ctgctgtttc tgcgctacat tagcgactgg     2580

gacctggacc ccggtcgctg ctaccgcgtg acctggttta ccagctggag cccgtgctat     2640

gattgcgcgc gtcacgtggc cgatttcctg cgcggtaacc cgaacctgag cctgcgcatt     2700

ttcaccgcgc gcctgtattt ctgcgaggat cgcaaggcgg agccggaggg cctgcgtcgt     2760

ctgcaccgtg ctggtgtgca gattgcgatt atgaccttta aggactactt ttattgctgg     2820

aacacctttg tggagaacca cgagcgcacc ttcaaagcgt gggagggtct gcacgagaac     2880

agcgtgcgtc tgagccgcca gctgcgtcgc atcctgctgc cgggcggtag cggcggtagc     2940

ggtggtagcg gcggcagcgg tggtagcggt ggcagcggtg gtatcgagac agagcaggag     3000

tacaaggaga ttttcatcac acctcaccag atcaagcaca tcaaggactt caaggactac     3060

aagtattctc acagggtgga taagaagccc aaccgcgagc tgatcaatga caccctgtat     3120

agcacacgga aggacgataa gggcaatacc ctgatcgtga acaatctgaa cggcctgtac     3180

gacaaggata atgacaagct gaagaagctg atcaacaagt ctcccgagaa gctgctgatg     3240

taccaccacg atcctcagac atatcagaag ctgaagctga tcatggagca gtacggcgac     3300

gagaagaacc cactgtataa gtactatgag gagacaggca actacctgac aaagtatagc     3360

aagaaggata atggccccgt gatcaagaag atcaagtact atggcaacaa gctgaatgcc     3420

cacctggaca tcaccgacga ttaccctaac tctcgcaata aggtggtgaa gctgagcctg     3480

aagccatacc ggttcgacgt gtacctggac aacggcgtgt ataagtttgt gacagtgaag     3540

aatctggatg tgatcaagaa ggagaactac tatgaggtga acagcaagtg ctacgaggag     3600

gccaagaagc tgaagaagat cagcaaccag gccgagttca tcgcctcttt ttacaacaat     3660

gacctgatca agatcaatgg cgagctgtat agagtgatcg gcgtgaacaa tgatctgctg     3720

aacagaatcg aagtgaatat gatcgacatc acctacaggg agtatctgga gaacatgaat     3780

gataagaggc cccctcgcat catcaagacc atcgcctcta agacacagag catcaagaag     3840

tacagcacag acatcctggg gaacctgtat gaagtcaaga gcaagaaaca tcctcagatt     3900

atcaagaaag gctctggtgg ttctaccaac ctgagcgaca tcattgagaa agagaccggc     3960

aagcagctgg taatccagga aagcattctg atgctgccgg aagaagtgga ggaagtgatt     4020

ggtaacaaac cggaaagcga catcctggtg cacaccgctt atgacgagag caccgacgaa     4080

aacgtgatgc tgctgaccag cgacgcgccg gagtataagc cgtgggcgct ggtaattcag     4140

gatagcaacg gcgaaaacaa aatcaagatg ctgtctggtg gttctaagcg acctgccgcc     4200

acaaagaagg ctggacaggc taagaagaag aaagattaca aagacgatga cgataaggga     4260

tccggcgcaa caaacttctc tctgctgaaa caagccggag atgtcgaaga gaatcctgga     4320

ccgatggcca agcctttgtc tcaagaagaa tccaccctca ttgaaagagc aacggctaca     4380

atcaacagca tccccatctc tgaagactac agcgtcgcca gcgcagctct ctctagcgac     4440

ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg tgcagaactc     4500

gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc     4560

ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt gcttctcgat     4620

ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac ggcagttggg     4680

attcgtgaat tgctgccctc tggttatgtg tgggagggct aa                        4722


<210>  151
<211>  4722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nSaCas9 N730 AIDx

<400>  151
gctagcgcca ccatgaagcg aaattacatt ctggggctgg ccattggcat tacatcagtg       60

ggctatggca tcattgacta cgagacaagg gacgtgatcg acgccggcgt gagactgttc      120

aaggaggcca acgtggagaa caatgagggc cggagatcca agaggggagc aaggcgcctg      180

aagcggagaa ggcgccacag aatccagaga gtgaagaagc tgctgttcga ttacaacctg      240

ctgaccgacc actccgagct gtctggcatc aatccttatg aggccagagt gaagggcctg      300

tcccagaagc tgtctgagga ggagtttagc gccgccctgc tgcacctggc aaagaggaga      360

ggcgtgcaca acgtgaatga ggtggaggag gacaccggca acgagctgtc cacaaaggag      420

cagatcagcc gcaattccaa ggccctggag gagaagtatg tggccgagct gcagctggag      480

cggctgaaga aggatggcga ggtgaggggc tccatcaatc gcttcaagac ctctgactac      540

gtgaaggagg ccaagcagct gctgaaggtg cagaaggcct accaccagct ggatcagtcc      600

tttatcgata catatatcga cctgctggag acaaggcgca catactatga gggaccagga      660

gagggctctc ccttcggctg gaaggacatc aaggagtggt acgagatgct gatgggccac      720

tgcacctatt ttccagagga gctgagaagc gtgaagtacg cctataacgc cgatctgtac      780

aacgccctga atgacctgaa caacctggtc atcaccaggg atgagaacga gaagctggag      840

tactatgaga agttccagat catcgagaac gtgttcaagc agaagaagaa gcctacactg      900

aagcagatcg ccaaggagat cctggtgaac gaggaggaca tcaagggcta ccgcgtgacc      960

tccacaggca agccagagtt caccaatctg aaggtgtatc acgatatcaa ggacatcaca     1020

gcccggaagg agatcatcga gaacgccgag ctgctggatc agatcgccaa gatcctgacc     1080

atctatcaga gctccgagga catccaggag gagctgacca acctgaatag cgagctgaca     1140

caggaggaga tcgagcagat cagcaatctg aagggctaca ccggcacaca caacctgagc     1200

ctgaaggcca tcaatctgat cctggatgag ctgtggcaca caaacgacaa tcagatcgcc     1260

atctttaacc ggctgaagct ggtgccaaag aaggtggacc tgtcccagca gaaggagatc     1320

ccaaccacac tggtggacga tttcatcctg tctcccgtgg tgaagcggag cttcatccag     1380

agcatcaaag tgatcaacgc catcatcaag aagtacggcc tgcccaatga tatcatcatc     1440

gagctggcca gggagaagaa ctccaaggac gcccagaaga tgatcaatga gatgcagaag     1500

aggaaccgcc agaccaatga gcggatcgag gagatcatca gaaccacagg caaggagaac     1560

gccaagtacc tgatcgagaa gatcaagctg cacgatatgc aggagggcaa gtgtctgtat     1620

tctctggagg ccatccctct ggaggacctg ctgaacaatc cattcaacta cgaggtggat     1680

cacatcatcc cccggagcgt gagcttcgac aattctttta acaataaggt gctggtgaag     1740

caggaggaga acagcaagaa gggcaatagg acccctttcc agtacctgtc tagctccgat     1800

tctaagatca gctacgagac attcaagaag cacatcctga atctggccaa gggcaagggc     1860

cgcatcagca agaccaagaa ggagtacctg ctggaggagc gggacatcaa cagattctcc     1920

gtgcagaagg acttcatcaa ccggaatctg gtggacacca gatacgccac acgcggcctg     1980

atgaatctgc tgcggtctta tttcagagtg aacaatctgg atgtgaaggt gaagagcatc     2040

aacggcggct tcacctcctt tctgcggaga aagtggaagt ttaagaagga gcgcaacaag     2100

ggctataagc accacgccga ggatgccctg atcatcgcca atgccgactt catctttaag     2160

gagtggaaga agctggacaa ggccaagaaa gtgatggaga acccgaagaa gaaacgtaag     2220

gtgggtagca gcggtagccc gaaaaagaag cgcaaagtgg gcagcgacgc gctggacgat     2280

ttcgatctgg acatgctggg cagcgatgcg ctggatgatt tcggcggtgg cagcatggat     2340

agcctgctga tgaaccgtcg caagttcctg taccagttca aaaacgtgcg ttgggcgaag     2400

ggtcgtcgtg agacctacct gtgctacgtg gtgaagcgtc gtgacagcgc gaccagcttc     2460

agcctggatt ttggctatct gcgtaacaaa aacggttgcc acgtggaact gctgtttctg     2520

cgctacatta gcgactggga cctggacccc ggtcgctgct accgcgtgac ctggtttacc     2580

agctggagcc cgtgctatga ttgcgcgcgt cacgtggccg atttcctgcg cggtaacccg     2640

aacctgagcc tgcgcatttt caccgcgcgc ctgtatttct gcgaggatcg caaggcggag     2700

ccggagggcc tgcgtcgtct gcaccgtgct ggtgtgcaga ttgcgattat gacctttaag     2760

gactactttt attgctggaa cacctttgtg gagaaccacg agcgcacctt caaagcgtgg     2820

gagggtctgc acgagaacag cgtgcgtctg agccgccagc tgcgtcgcat cctgctgccg     2880

ggcggtagcg gcggtagcgg tggtagcggc ggcagcggtg gtagcggtgg cagcggtggt     2940

aaccagatgt tcgaggagaa gcaggccgag agcatgcccg agatcgagac agagcaggag     3000

tacaaggaga ttttcatcac acctcaccag atcaagcaca tcaaggactt caaggactac     3060

aagtattctc acagggtgga taagaagccc aaccgcgagc tgatcaatga caccctgtat     3120

agcacacgga aggacgataa gggcaatacc ctgatcgtga acaatctgaa cggcctgtac     3180

gacaaggata atgacaagct gaagaagctg atcaacaagt ctcccgagaa gctgctgatg     3240

taccaccacg atcctcagac atatcagaag ctgaagctga tcatggagca gtacggcgac     3300

gagaagaacc cactgtataa gtactatgag gagacaggca actacctgac aaagtatagc     3360

aagaaggata atggccccgt gatcaagaag atcaagtact atggcaacaa gctgaatgcc     3420

cacctggaca tcaccgacga ttaccctaac tctcgcaata aggtggtgaa gctgagcctg     3480

aagccatacc ggttcgacgt gtacctggac aacggcgtgt ataagtttgt gacagtgaag     3540

aatctggatg tgatcaagaa ggagaactac tatgaggtga acagcaagtg ctacgaggag     3600

gccaagaagc tgaagaagat cagcaaccag gccgagttca tcgcctcttt ttacaacaat     3660

gacctgatca agatcaatgg cgagctgtat agagtgatcg gcgtgaacaa tgatctgctg     3720

aacagaatcg aagtgaatat gatcgacatc acctacaggg agtatctgga gaacatgaat     3780

gataagaggc cccctcgcat catcaagacc atcgcctcta agacacagag catcaagaag     3840

tacagcacag acatcctggg gaacctgtat gaagtcaaga gcaagaaaca tcctcagatt     3900

atcaagaaag gctctggtgg ttctaccaac ctgagcgaca tcattgagaa agagaccggc     3960

aagcagctgg taatccagga aagcattctg atgctgccgg aagaagtgga ggaagtgatt     4020

ggtaacaaac cggaaagcga catcctggtg cacaccgctt atgacgagag caccgacgaa     4080

aacgtgatgc tgctgaccag cgacgcgccg gagtataagc cgtgggcgct ggtaattcag     4140

gatagcaacg gcgaaaacaa aatcaagatg ctgtctggtg gttctaagcg acctgccgcc     4200

acaaagaagg ctggacaggc taagaagaag aaagattaca aagacgatga cgataaggga     4260

tccggcgcaa caaacttctc tctgctgaaa caagccggag atgtcgaaga gaatcctgga     4320

ccgatggcca agcctttgtc tcaagaagaa tccaccctca ttgaaagagc aacggctaca     4380

atcaacagca tccccatctc tgaagactac agcgtcgcca gcgcagctct ctctagcgac     4440

ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg tgcagaactc     4500

gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc     4560

ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt gcttctcgat     4620

ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac ggcagttggg     4680

attcgtgaat tgctgccctc tggttatgtg tgggagggct aa                        4722


<210>  152
<211>  7502
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Px601 SCP1_nSaCas9_I744_miniABEMax_V82G hU6_sgRNA_site11

<400>  152
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt       60

ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact      120

aggggttcct gcggcctcta gatcgaggta cttatataag ggggtggggg cgcgttcgtc      180

ctcagtcgcg atcgaacact cgagccgagc agacgtgcct acggaccgac cggtgctagc      240

gccaccatga agcgaaatta cattctgggg ctggccattg gcattacatc agtgggctat      300

ggcatcattg actacgagac aagggacgtg atcgacgccg gcgtgagact gttcaaggag      360

gccaacgtgg agaacaatga gggccggaga tccaagaggg gagcaaggcg cctgaagcgg      420

agaaggcgcc acagaatcca gagagtgaag aagctgctgt tcgattacaa cctgctgacc      480

gaccactccg agctgtctgg catcaatcct tatgaggcca gagtgaaggg cctgtcccag      540

aagctgtctg aggaggagtt tagcgccgcc ctgctgcacc tggcaaagag gagaggcgtg      600

cacaacgtga atgaggtgga ggaggacacc ggcaacgagc tgtccacaaa ggagcagatc      660

agccgcaatt ccaaggccct ggaggagaag tatgtggccg agctgcagct ggagcggctg      720

aagaaggatg gcgaggtgag gggctccatc aatcgcttca agacctctga ctacgtgaag      780

gaggccaagc agctgctgaa ggtgcagaag gcctaccacc agctggatca gtcctttatc      840

gatacatata tcgacctgct ggagacaagg cgcacatact atgagggacc aggagagggc      900

tctcccttcg gctggaagga catcaaggag tggtacgaga tgctgatggg ccactgcacc      960

tattttccag aggagctgag aagcgtgaag tacgcctata acgccgatct gtacaacgcc     1020

ctgaatgacc tgaacaacct ggtcatcacc agggatgaga acgagaagct ggagtactat     1080

gagaagttcc agatcatcga gaacgtgttc aagcagaaga agaagcctac actgaagcag     1140

atcgccaagg agatcctggt gaacgaggag gacatcaagg gctaccgcgt gacctccaca     1200

ggcaagccag agttcaccaa tctgaaggtg tatcacgata tcaaggacat cacagcccgg     1260

aaggagatca tcgagaacgc cgagctgctg gatcagatcg ccaagatcct gaccatctat     1320

cagagctccg aggacatcca ggaggagctg accaacctga atagcgagct gacacaggag     1380

gagatcgagc agatcagcaa tctgaagggc tacaccggca cacacaacct gagcctgaag     1440

gccatcaatc tgatcctgga tgagctgtgg cacacaaacg acaatcagat cgccatcttt     1500

aaccggctga agctggtgcc aaagaaggtg gacctgtccc agcagaagga gatcccaacc     1560

acactggtgg acgatttcat cctgtctccc gtggtgaagc ggagcttcat ccagagcatc     1620

aaagtgatca acgccatcat caagaagtac ggcctgccca atgatatcat catcgagctg     1680

gccagggaga agaactccaa ggacgcccag aagatgatca atgagatgca gaagaggaac     1740

cgccagacca atgagcggat cgaggagatc atcagaacca caggcaagga gaacgccaag     1800

tacctgatcg agaagatcaa gctgcacgat atgcaggagg gcaagtgtct gtattctctg     1860

gaggccatcc ctctggagga cctgctgaac aatccattca actacgaggt ggatcacatc     1920

atcccccgga gcgtgagctt cgacaattct tttaacaata aggtgctggt gaagcaggag     1980

gagaacagca agaagggcaa taggacccct ttccagtacc tgtctagctc cgattctaag     2040

atcagctacg agacattcaa gaagcacatc ctgaatctgg ccaagggcaa gggccgcatc     2100

agcaagacca agaaggagta cctgctggag gagcgggaca tcaacagatt ctccgtgcag     2160

aaggacttca tcaaccggaa tctggtggac accagatacg ccacacgcgg cctgatgaat     2220

ctgctgcggt cttatttcag agtgaacaat ctggatgtga aggtgaagag catcaacggc     2280

ggcttcacct cctttctgcg gagaaagtgg aagtttaaga aggagcgcaa caagggctat     2340

aagcaccacg ccgaggatgc cctgatcatc gccaatgccg acttcatctt taaggagtgg     2400

aagaagctgg acaaggccaa gaaagtgatg gagaaccaga tgttcgagga gaagcaggcc     2460

gagagcatgc ccgagatccc gaagaagaaa cgtaaggtgg gtagcagcgg tagcccgaaa     2520

aagaagcgca aagtgggcag cgacgcgctg gacgatttcg atctggacat gctgggcagc     2580

gatgcgctgg atgatttcgg cggtggcagc tctgaggtgg agttttccca cgagtactgg     2640

atgagacatg ccctgaccct ggccaagagg gcacgcgatg agagggaggt gcctgtggga     2700

gccgtgctgg tgctgaacaa tagagtgatc ggcgagggct ggaacagagc catcggcctg     2760

cacgacccaa cagcccatgc cgaaattatg gccctgagac agggcggcct ggtcatgcag     2820

aactacagac tgattgacgc caccctgtac ggcacattcg agccttgcgt gatgtgcgcc     2880

ggcgccatga tccactctag gatcggccgc gtggtgtttg gcgtgaggaa cgcaaaaacc     2940

ggcgccgcag gctccctgat ggacgtgctg cactaccccg gcatgaatca ccgcgtcgaa     3000

attaccgagg gaatcctggc agatgaatgt gccgccctgc tgtgctattt ctttcggatg     3060

cctagacagg tgttcaatgc tcagaagaag gcccagagct ccaccgacgg cggtagcggc     3120

ggtagcggtg gtagcggcgg cagcggtggt agcggtggca gcggtggtat cgagacagag     3180

caggagtaca aggagatttt catcacacct caccagatca agcacatcaa ggacttcaag     3240

gactacaagt attctcacag ggtggataag aagcccaacc gcgagctgat caatgacacc     3300

ctgtatagca cacggaagga cgataagggc aataccctga tcgtgaacaa tctgaacggc     3360

ctgtacgaca aggataatga caagctgaag aagctgatca acaagtctcc cgagaagctg     3420

ctgatgtacc accacgatcc tcagacatat cagaagctga agctgatcat ggagcagtac     3480

ggcgacgaga agaacccact gtataagtac tatgaggaga caggcaacta cctgacaaag     3540

tatagcaaga aggataatgg ccccgtgatc aagaagatca agtactatgg caacaagctg     3600

aatgcccacc tggacatcac cgacgattac cctaactctc gcaataaggt ggtgaagctg     3660

agcctgaagc cataccggtt cgacgtgtac ctggacaacg gcgtgtataa gtttgtgaca     3720

gtgaagaatc tggatgtgat caagaaggag aactactatg aggtgaacag caagtgctac     3780

gaggaggcca agaagctgaa gaagatcagc aaccaggccg agttcatcgc ctctttttac     3840

aacaatgacc tgatcaagat caatggcgag ctgtatagag tgatcggcgt gaacaatgat     3900

ctgctgaaca gaatcgaagt gaatatgatc gacatcacct acagggagta tctggagaac     3960

atgaatgata agaggccccc tcgcatcatc aagaccatcg cctctaagac acagagcatc     4020

aagaagtaca gcacagacat cctggggaac ctgtatgaag tcaagagcaa gaaacatcct     4080

cagattatca agaaaggctc tggtggttct aagcgacctg ccgccacaaa gaaggctgga     4140

caggctaaga agaagaaaga ttacaaagac gatgacgata agtaaggatc cctgtgcctt     4200

ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg     4260

ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt     4320

gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaga     4380

atagcaggca tgctggggag gtaccgaggg cctatttccc atgattcctt catatttgca     4440

tatacgatac aaggctgtta gagagataat tggaattaat ttgactgtaa acacaaagat     4500

attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa     4560

attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt     4620

ggctttatat atcttgtgga aaggacgaaa caccgggagc tagacaaaat ggagtgtttt     4680

agtactctgg aaacagaatc tactaaaaca aggcaaaatg ccgtgtttat ctcgtcaact     4740

tgttggcgag atttttgcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc     4800

tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg     4860

cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt     4920

attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta     4980

cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc     5040

tacacttgcc agcgccttag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac     5100

gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag     5160

tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc     5220

atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg     5280

actcttgttc caaactggaa caacactcaa ctctatctcg ggctattctt ttgatttata     5340

agggattttg ccgatttcgg tctattggtt aaaaaatgag ctgatttaac aaaaatttaa     5400

cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg     5460

ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg     5520

acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg     5580

catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat     5640

acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac     5700

ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat     5760

gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag     5820

tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc     5880

tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc     5940

acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc     6000

cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc     6060

ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt     6120

ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt     6180

atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat     6240

cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct     6300

tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat     6360

gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc     6420

ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg     6480

ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtggaag     6540

ccgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta     6600

cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc     6660

ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga     6720

tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat     6780

gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat     6840

caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa     6900

accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa     6960

ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt     7020

aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt     7080

accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata     7140

gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt     7200

ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac     7260

gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga     7320

gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg     7380

ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa     7440

aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat     7500

gt                                                                    7502


<210>  153
<211>  7501
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Px601 SCP1_nSaCas9_I744_miniABEMax_V82G hU6_sgRNA_LacZ

<400>  153
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt       60

ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact      120

aggggttcct gcggcctcta gatcgaggta cttatataag ggggtggggg cgcgttcgtc      180

ctcagtcgcg atcgaacact cgagccgagc agacgtgcct acggaccgac cggtgctagc      240

gccaccatga agcgaaatta cattctgggg ctggccattg gcattacatc agtgggctat      300

ggcatcattg actacgagac aagggacgtg atcgacgccg gcgtgagact gttcaaggag      360

gccaacgtgg agaacaatga gggccggaga tccaagaggg gagcaaggcg cctgaagcgg      420

agaaggcgcc acagaatcca gagagtgaag aagctgctgt tcgattacaa cctgctgacc      480

gaccactccg agctgtctgg catcaatcct tatgaggcca gagtgaaggg cctgtcccag      540

aagctgtctg aggaggagtt tagcgccgcc ctgctgcacc tggcaaagag gagaggcgtg      600

cacaacgtga atgaggtgga ggaggacacc ggcaacgagc tgtccacaaa ggagcagatc      660

agccgcaatt ccaaggccct ggaggagaag tatgtggccg agctgcagct ggagcggctg      720

aagaaggatg gcgaggtgag gggctccatc aatcgcttca agacctctga ctacgtgaag      780

gaggccaagc agctgctgaa ggtgcagaag gcctaccacc agctggatca gtcctttatc      840

gatacatata tcgacctgct ggagacaagg cgcacatact atgagggacc aggagagggc      900

tctcccttcg gctggaagga catcaaggag tggtacgaga tgctgatggg ccactgcacc      960

tattttccag aggagctgag aagcgtgaag tacgcctata acgccgatct gtacaacgcc     1020

ctgaatgacc tgaacaacct ggtcatcacc agggatgaga acgagaagct ggagtactat     1080

gagaagttcc agatcatcga gaacgtgttc aagcagaaga agaagcctac actgaagcag     1140

atcgccaagg agatcctggt gaacgaggag gacatcaagg gctaccgcgt gacctccaca     1200

ggcaagccag agttcaccaa tctgaaggtg tatcacgata tcaaggacat cacagcccgg     1260

aaggagatca tcgagaacgc cgagctgctg gatcagatcg ccaagatcct gaccatctat     1320

cagagctccg aggacatcca ggaggagctg accaacctga atagcgagct gacacaggag     1380

gagatcgagc agatcagcaa tctgaagggc tacaccggca cacacaacct gagcctgaag     1440

gccatcaatc tgatcctgga tgagctgtgg cacacaaacg acaatcagat cgccatcttt     1500

aaccggctga agctggtgcc aaagaaggtg gacctgtccc agcagaagga gatcccaacc     1560

acactggtgg acgatttcat cctgtctccc gtggtgaagc ggagcttcat ccagagcatc     1620

aaagtgatca acgccatcat caagaagtac ggcctgccca atgatatcat catcgagctg     1680

gccagggaga agaactccaa ggacgcccag aagatgatca atgagatgca gaagaggaac     1740

cgccagacca atgagcggat cgaggagatc atcagaacca caggcaagga gaacgccaag     1800

tacctgatcg agaagatcaa gctgcacgat atgcaggagg gcaagtgtct gtattctctg     1860

gaggccatcc ctctggagga cctgctgaac aatccattca actacgaggt ggatcacatc     1920

atcccccgga gcgtgagctt cgacaattct tttaacaata aggtgctggt gaagcaggag     1980

gagaacagca agaagggcaa taggacccct ttccagtacc tgtctagctc cgattctaag     2040

atcagctacg agacattcaa gaagcacatc ctgaatctgg ccaagggcaa gggccgcatc     2100

agcaagacca agaaggagta cctgctggag gagcgggaca tcaacagatt ctccgtgcag     2160

aaggacttca tcaaccggaa tctggtggac accagatacg ccacacgcgg cctgatgaat     2220

ctgctgcggt cttatttcag agtgaacaat ctggatgtga aggtgaagag catcaacggc     2280

ggcttcacct cctttctgcg gagaaagtgg aagtttaaga aggagcgcaa caagggctat     2340

aagcaccacg ccgaggatgc cctgatcatc gccaatgccg acttcatctt taaggagtgg     2400

aagaagctgg acaaggccaa gaaagtgatg gagaaccaga tgttcgagga gaagcaggcc     2460

gagagcatgc ccgagatccc gaagaagaaa cgtaaggtgg gtagcagcgg tagcccgaaa     2520

aagaagcgca aagtgggcag cgacgcgctg gacgatttcg atctggacat gctgggcagc     2580

gatgcgctgg atgatttcgg cggtggcagc tctgaggtgg agttttccca cgagtactgg     2640

atgagacatg ccctgaccct ggccaagagg gcacgcgatg agagggaggt gcctgtggga     2700

gccgtgctgg tgctgaacaa tagagtgatc ggcgagggct ggaacagagc catcggcctg     2760

cacgacccaa cagcccatgc cgaaattatg gccctgagac agggcggcct ggtcatgcag     2820

aactacagac tgattgacgc caccctgtac ggcacattcg agccttgcgt gatgtgcgcc     2880

ggcgccatga tccactctag gatcggccgc gtggtgtttg gcgtgaggaa cgcaaaaacc     2940

ggcgccgcag gctccctgat ggacgtgctg cactaccccg gcatgaatca ccgcgtcgaa     3000

attaccgagg gaatcctggc agatgaatgt gccgccctgc tgtgctattt ctttcggatg     3060

cctagacagg tgttcaatgc tcagaagaag gcccagagct ccaccgacgg cggtagcggc     3120

ggtagcggtg gtagcggcgg cagcggtggt agcggtggca gcggtggtat cgagacagag     3180

caggagtaca aggagatttt catcacacct caccagatca agcacatcaa ggacttcaag     3240

gactacaagt attctcacag ggtggataag aagcccaacc gcgagctgat caatgacacc     3300

ctgtatagca cacggaagga cgataagggc aataccctga tcgtgaacaa tctgaacggc     3360

ctgtacgaca aggataatga caagctgaag aagctgatca acaagtctcc cgagaagctg     3420

ctgatgtacc accacgatcc tcagacatat cagaagctga agctgatcat ggagcagtac     3480

ggcgacgaga agaacccact gtataagtac tatgaggaga caggcaacta cctgacaaag     3540

tatagcaaga aggataatgg ccccgtgatc aagaagatca agtactatgg caacaagctg     3600

aatgcccacc tggacatcac cgacgattac cctaactctc gcaataaggt ggtgaagctg     3660

agcctgaagc cataccggtt cgacgtgtac ctggacaacg gcgtgtataa gtttgtgaca     3720

gtgaagaatc tggatgtgat caagaaggag aactactatg aggtgaacag caagtgctac     3780

gaggaggcca agaagctgaa gaagatcagc aaccaggccg agttcatcgc ctctttttac     3840

aacaatgacc tgatcaagat caatggcgag ctgtatagag tgatcggcgt gaacaatgat     3900

ctgctgaaca gaatcgaagt gaatatgatc gacatcacct acagggagta tctggagaac     3960

atgaatgata agaggccccc tcgcatcatc aagaccatcg cctctaagac acagagcatc     4020

aagaagtaca gcacagacat cctggggaac ctgtatgaag tcaagagcaa gaaacatcct     4080

cagattatca agaaaggctc tggtggttct aagcgacctg ccgccacaaa gaaggctgga     4140

caggctaaga agaagaaaga ttacaaagac gatgacgata agtaaggatc cctgtgcctt     4200

ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg     4260

ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt     4320

gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaga     4380

atagcaggca tgctggggag gtaccgaggg cctatttccc atgattcctt catatttgca     4440

tatacgatac aaggctgtta gagagataat tggaattaat ttgactgtaa acacaaagat     4500

attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa     4560

attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt     4620

ggctttatat atcttgtgga aaggacgaaa cacctgcgaa tacgcccacg cgatgtttta     4680

gtactctgga aacagaatct actaaaacaa ggcaaaatgc cgtgtttatc tcgtcaactt     4740

gttggcgaga tttttgcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct     4800

gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc     4860

ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta     4920

ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac     4980

gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct     5040

acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg     5100

ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt     5160

gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca     5220

tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga     5280

ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa     5340

gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac     5400

gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc     5460

tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga     5520

cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc     5580

atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata     5640

cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact     5700

tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg     5760

tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt     5820

atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct     5880

gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca     5940

cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc     6000

gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc     6060

cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg     6120

gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta     6180

tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc     6240

ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt     6300

gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg     6360

cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct     6420

tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc     6480

tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtggaagc     6540

cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac     6600

acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc     6660

tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat     6720

ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg     6780

accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc     6840

aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa     6900

ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag     6960

gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta     7020

ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta     7080

ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag     7140

ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg     7200

gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg     7260

cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag     7320

cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc     7380

cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa     7440

aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg     7500

t                                                                     7501


