                         SEQUENCE LISTING

<110>  Amgen Inc.
 
<120>  DETERMINATION OF UNPEGGYLATED FREE N-TERMINUS OF PEGFILGRASTIM 
       USING AN ACID PROTEASE

<130>  01017/55200

<150>  US 62/992,540
<151>  2020-03-20

<160>  2     

<170>  PatentIn version 3.5

<210>  1
<211>  175
<212>  PRT
<213>  Pegfilgrastim

<400>  1

Met Thr Pro Leu Gly Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu 
1               5                   10                  15      


Lys Cys Leu Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu 
            20                  25                  30          


Gln Glu Lys Leu Cys Ala Thr Tyr Lys Leu Cys His Pro Glu Glu Leu 
        35                  40                  45              


Val Leu Leu Gly His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser 
    50                  55                  60                  


Cys Pro Ser Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser Gln Leu His 
65                  70                  75                  80  


Ser Gly Leu Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu Gly Ile 
                85                  90                  95      


Ser Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Ala 
            100                 105                 110         


Asp Phe Ala Thr Thr Ile Trp Gln Gln Met Glu Glu Leu Gly Met Ala 
        115                 120                 125             


Pro Ala Leu Gln Pro Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala 
    130                 135                 140                 


Phe Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His Leu Gln Ser 
145                 150                 155                 160 


Phe Leu Glu Val Ser Tyr Arg Val Leu Arg His Leu Ala Gln Pro 
                165                 170                 175 


<210>  2
<211>  17
<212>  PRT
<213>  Pegfilgrastim

<400>  2

Met Thr Pro Leu Gly Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu 
1               5                   10                  15      


Lys 
    


