                         SEQUENCE LISTING

<110>  Duke University
 
<120>  METHODS AND COMPOSITIONS FOR IMPROVED TYPE I-E CRISPR BASED GENE 
       SILENCING

<130>  49186-45

<150>  62/990,172
<151>  2020-03-16

<160>  61    

<170>  PatentIn version 3.5

<210>  1
<211>  112
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic guide array assembly

<400>  1
aagttatgaa gttcgagttc cccgcgccag cggggataaa ccgtattgac caattcattc       60

ggacagttat tagttcgagt tccccgcgcc agcggggata aaccgttaca tt              112


<210>  2
<211>  93
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic guide array assembly

<400>  2
tcgagttccc cgcgccagcg gggataaacc gtattgacca attcattcgg acagttatta       60

gttcgagttc cccgcgccag cggggataaa ccg                                    93


<210>  3
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic guide array assembly

<400>  3
agttccccgc gccagcgggg ataaaccgta ttgaccaatt cattcggaca gttattagtt       60

cgagttcccc gcgccagcgg ggataaaccg                                        90


<210>  4
<211>  89
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic guide array assembly

<400>  4
gttccccgcg ccagcgggga taaaccgtat tgaccaattc attcggacag ttattagttc       60

gagttccccg cgccagcggg gataaaccg                                         89


<210>  5
<211>  88
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic guide array assembly

<400>  5
ttccccgcgc cagcggggat aaaccgtatt gaccaattca ttcggacagt tattagttcg       60

agttccccgc gccagcgggg ataaaccg                                          88


<210>  6
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic guide array assembly

<400>  6
attgaccaat tcattcggac agttattagt tcgagttccc cgcgccagcg gggataaacc       60

g                                                                       61


<210>  7
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic guide array assembly

<400>  7
tcgagttccc cgcgccagcg gggataaacc gtattgacca attcattcgg acagttatta       60

gttcgag                                                                 67


<210>  8
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic guide array assembly

<400>  8
ttccccgcgc cagcggggat aaaccgtatt gaccaattca ttcggacagt tattagttcg       60

ag                                                                      62


<210>  9
<211>  92
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sgRNA guide sequences

<400>  9
tcgagttccc cgcgccagcg gggataaacc gttgattata ataaccgttt atctgttcgt       60

atcgagttcc ccgcgccagc ggggataaac cg                                     92


<210>  10
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  10
gtttatctgt tcgtatcgag ttccccgcgc cagcggggat aaaccgaaaa aaaaacccc        59


<210>  11
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  11
ggttattata atcaacggtt tatccccgct ggcgcgggga actcgaggtg gtaccagatc       60


<210>  12
<211>  154
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sgRNA guide sequences

<400>  12
tcgagttccc cgcgccagcg gggataaacc gttgattata ataaccgttt atctgttcgt       60

atcgagttcc ccgcgccagc ggggataaac cgaaaagcat ataatgcgta aaagttatga      120

agttcgagtt ccccgcgcca gcggggataa accg                                  154


<210>  13
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  13
gcgccagcgg ggataaaccg aaaagcatat aatgcg                                 36


<210>  14
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic  primer

<400>  14
cttgcccgcc tgatgaatgc tcatccgg                                          28


<210>  15
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  15
ccggatgagc attcatcagg cgggcaag                                          28


<210>  16
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  16
cggtttatcc ccgctggcgc ggggaactcg atacgaacag ataaacggtt attataatc        59


<210>  17
<211>  155
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SyntheticsgRNA guide sequences

<400>  17
tcgagttccc cgcgccagcg gggataaacc gttgattata ataaccgttt atctgttcgt       60

atcgagttcc ccgcgccagc ggggataaac cgtattgacc aattcattcg ggacagttat      120

tagttcgagt tccccgcgcc agcggggata aaccg                                 155


<210>  18
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  18
gcgccagcgg ggataaaccg tattgaccaa ttcattc                                37


<210>  19
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  19
cttgcccgcc tgatgaatgc tcatccgg                                          28


<210>  20
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic  primer

<400>  20
ccggatgagc attcatcagg cgggcaag                                          28


<210>  21
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic  primer

<400>  21
cggtttatcc ccgctggcgc ggggaactcg atacgaacag ataaacggtt attataatc        59


<210>  22
<211>  153
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sgRNA guide sequences

<400>  22
tcgagttccc cgcgccagcg gggataaacc gttgattata ataaccgttt atctgttcgt       60

atcgagttcc ccgcgccagc ggggataaac cgttaccatt ctgttgcttt tatgtataag      120

aatcgagttc cccgcgccag cggggataaa ccg                                   153


<210>  23
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  23
gcgccagcgg ggataaaccg ttaccattct gttg                                   34


<210>  24
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  24
cttgcccgcc tgatgaatgc tcatccgg                                          28


<210>  25
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  25
ccggatgagc attcatcagg cgggcaag                                          28


<210>  26
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  26
cggtttatcc ccgctggcgc ggggaactcg atacgaacag ataaacggtt attataatc        59


<210>  27
<211>  153
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sgRNA guide sequences

<400>  27
tcgagttccc cgcgccagcg gggataaacc gttgattata ataaccgttt atctgttcgt       60

atcgagttcc ccgcgccagc ggggataaac cgctcgtaaa agcagtacag tgcaccgtaa      120

gatcgagttc cccgcgccag cggggataaa ccg                                   153


<210>  28
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  28
gcgccagcgg ggataaaccg ctcgtaaaag                                        30


<210>  29
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  29
cttgcccgcc tgatgaatgc tcatccgg                                          28


<210>  30
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  30
ccggatgagc attcatcagg cgggcaag                                          28


<210>  31
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  31
cggtttatcc ccgctggcgc ggggaactcg atacgaacag ataaacggtt attataatc        59


<210>  32
<211>  217
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sgRNA guide sequences

<400>  32
tcgagttccc cgcgccagcg gggataaacc gttgattata ataaccgttt atctgttcgt       60

atcgagttcc ccgcgccagc ggggataaac cgaaaagcat ataatgcgta aaagttatga      120

agttcgagtt ccccgcgcca gcggggataa accgtattga ccaattcatt cgggacagtt      180

attagttcga gttccccgcg ccagcgggga taaaccg                               217


<210>  33
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  33
gcgccagcgg ggataaaccg tattgaccaa ttcattc                                37


<210>  34
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  34
cttgcccgcc tgatgaatgc tcatccgg                                          28


<210>  35
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  35
ccggatgagc attcatcagg cgggcaag                                          28


<210>  36
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  36
cggtttatcc ccgctggcgc ggggaactcg aacttcataa cttttac                     47


<210>  37
<211>  278
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sgRNA guide sequences

<400>  37
tcgagttccc cgcgccagcg gggataaacc gttgattata ataaccgttt atctgttcgt       60

atcgagttcc ccgcgccagc ggggataaac cgaaaagcat ataatgcgta aaagttatga      120

agttcgagtt ccccgcgcca gcggggataa accgtattga ccaattcatt cgggacagtt      180

attagttcga gttccccgcg ccagcgggga taaaccgtta ccattctgtt gcttttatgt      240

ataagaatcg agttccccgc gccagcgggg ataaaccg                              278


<210>  38
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  38
gcgccagcgg ggataaaccg tattgaccaa ttcattc                                37


<210>  39
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  39
cttgcccgcc tgatgaatgc tcatccgg                                          28


<210>  40
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  40
ccggatgagc attcatcagg cgggcaag                                          28


<210>  41
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  41
cggtttatcc ccgctggcgc ggggaactcg aacttcataa cttttac                     47


<210>  42
<211>  278
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sgRNA guide sequences

<400>  42
tcgagttccc cgcgccagcg gggataaacc gttgattata ataaccgttt atctgttcgt       60

atcgagttcc ccgcgccagc ggggataaac cgaaaagcat ataatgcgta aaagttatga      120

agttcgagtt ccccgcgcca gcggggataa accgtattga ccaattcatt cgggacagtt      180

attagttcga gttccccgcg ccagcgggga taaaccgctc gtaaaagcag tacagtgcac      240

cgtaagatcg agttccccgc gccagcgggg ataaaccg                              278


<210>  43
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  43
gcgccagcgg ggataaaccg tattgaccaa ttcattc                                37


<210>  44
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  44
cttgcccgcc tgatgaatgc tcatccgg                                          28


<210>  45
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  45
ccggatgagc attcatcagg cgggcaag                                          28


<210>  46
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  46
cggtttatcc ccgctggcgc ggggaactcg aacttcataa cttttac                     47


<210>  47
<211>  339
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sgRNA guide sequences

<400>  47
tcgagttccc cgcgccagcg gggataaacc gttgattata ataaccgttt atctgttcgt       60

atcgagttcc ccgcgccagc ggggataaac cgaaaagcat ataatgcgta aaagttatga      120

agttcgagtt ccccgcgcca gcggggataa accgtattga ccaattcatt cgggacagtt      180

attagttcga gttccccgcg ccagcgggga taaaccgtta ccattctgtt gcttttatgt      240

ataagaatcg agttccccgc gccagcgggg ataaaccgct cgtaaaagca gtacagtgca      300

ccgtaagatc gagttccccg cgccagcggg gataaaccg                             339


<210>  48
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  48
gcgccagcgg ggataaaccg ctcgtaaaag                                        30


<210>  49
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  49
cttgcccgcc tgatgaatgc tcatccgg                                          28


<210>  50
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  50
ccggatgagc attcatcagg cgggcaag                                          28


<210>  51
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  51
cggtttatcc ccgctggcgc ggggaactcg attcttatac ataaaagc                    48


<210>  52
<211>  3527
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic plasmid

<400>  52
tcctaatttt tgttgacact ctatcattga tagagttatt ttaccactcc ctatcagtga       60

tagagaaaag tgaaatgaat agttcgacaa agatcgcatt ggtaattacg ttactcgatg      120

ccatggggat tggccttatc atgccagtct tgccaacgtt attacgtgaa tttattgctt      180

cggaagatat cgctaaccac tttggcgtat tgcttgcact ttatgcgtta atgcaggtta      240

tctttgctcc ttggcttgga aaaatgtctg accgatttgg tcggcgccca gtgctgttgt      300

tgtcattaat aggcgcatcg ctggattact tattgctggc tttttcaagt gcgctttgga      360

tgctgtattt aggccgtttg ctttcaggga tcacaggagc tactggggct gtcgcggcat      420

cggtcattgc cgataccacc tcagcttctc aacgcgtgaa gtggttcggt tggttagggg      480

caagttttgg gcttggttta atagcggggc ctattattgg tggttttgca ggagagattt      540

caccgcatag tccctttttt atcgctgcgt tgctaaatat tgtcactttc cttgtggtta      600

tgttttggtt ccgtgaaacc aaaaatacac gtgataatac agataccgaa gtaggggttg      660

agacgcaatc gaattcggta tacatcactt tatttaaaac gatgcccatt ttgttgatta      720

tttatttttc agcgcaattg ataggccaaa ttcccgcaac ggtgtgggtg ctatttaccg      780

aaaatcgttt tggatggaat agcatgatgg ttggcttttc attagcgggt cttggtcttt      840

tacactcagt attccaagcc tttgtggcag gaagaatagc cactaaatgg ggcgaaaaaa      900

cggcagtact gctcggattt attgcagata gtagtgcatt tgccttttta gcgtttatat      960

ctgaaggttg gttagttttc cctgttttaa ttttattggc tggtggtggg atcgctttac     1020

ctgcattaca gggagtgatg tctatccaaa caaagagtca tcagcaaggt gctttacagg     1080

gattattggt gagccttacc aatgcaaccg gtgttattgg cccattactg tttgctgtta     1140

tttataatca ttcactacca atttgggatg gctggatttg gattattggt ttagcgtttt     1200

actgtattat tatcctgcta tcgatgacct tcatgttaac ccctcaagct caggggagta     1260

aacaggagac aagtgcttag ttatttcgtc accaaatgat gttattccgc gaaatataat     1320

gaccctcttg ataacccaag agcatcacat atacctgccg ttcactatta tttagtgaaa     1380

tgagatatta tgatattttc tgaattgtga ttaaaaaggc aactttatgc ccatgcaaca     1440

gaaactataa aaaatacaga gaatgaaaag aaacagatag attttttagt tctttaggcc     1500

cgtagtctgc aaatcctttt atgattttct atcaaacaaa agaggaaaat agaccagttg     1560

caatccaaac gagagtctaa tagaatgagg tcgaaaagta aatcgcgcgg gtttgttact     1620

gataaagcag gcaagaccta aaatgtgtaa agggcaaagt gtatactttg gcgtcacccc     1680

ttacatattt taggtctttt tttattgtgc gtaactaact tgccatcttc aaacaggagg     1740

gctggaagaa gcagaccgct aacacagtac ataaaaaagg agacatgaac gatgaacatc     1800

aaaaagtttg caaaacaagc aacagtatta acctttacta ccgcactgct ggcaggaggc     1860

gcaactcaag cgtttgcgaa agaaacgaac caaaagccat ataaggaaac atacggcatt     1920

tcccatatta cacgccatga tatgctgcaa atccctgaac agcaaaaaaa tgaaaaatat     1980

caagttcctg agttcgattc gtccacaatt aaaaatatct cttctgcaaa aggcctggac     2040

gtttgggaca gctggccatt acaaaacgct gacggcactg tcgcaaacta tcacggctac     2100

cacatcgtct ttgcattagc cggagatcct aaaaatgcgg atgacacatc gatttacatg     2160

ttctatcaaa aagtcggcga aacttctatt gacagctgga aaaacgctgg ccgcgtcttt     2220

aaagacagcg acaaattcga tgcaaatgat tctatcctaa aagaccaaac acaagaatgg     2280

tcaggttcag ccacatttac atctgacgga aaaatccgtt tattctacac tgatttctcc     2340

ggtaaacatt acggcaaaca aacactgaca actgcacaag ttaacgtatc agcatcagac     2400

agctctttga acatcaacgg tgtagaggat tataaatcaa tctttgacgg tgacggaaaa     2460

acgtatcaaa atgtacagca gttcatcgat gaaggcaact acagctcagg cgacaaccat     2520

acgctgagag atcctcacta cgtagaagat aaaggccaca aatacttagt atttgaagca     2580

aacactggaa ctgaagatgg ctaccaaggc gaagaatctt tatttaacaa agcatactat     2640

ggcaaaagca catcattctt ccgtcaagaa agtcaaaaac ttctgcaaag cgataaaaaa     2700

cgcacggctg agttagcaaa cggcgctctc ggtatgattg agctaaacga tgattacaca     2760

ctgaaaaaag tgatgaaacc gctgattgca tctaacacag taacagatga aattgaacgc     2820

gcgaacgtct ttaaaatgaa cggcaaatgg tacctgttca ctgactcccg cggatcaaaa     2880

atgacgattg acggcattac gtctaacgat atttacatgc ttggttatgt ttctaattct     2940

ttaactggcc catacaagcc gctgaacaaa actggccttg tgttaaaaat ggatcttgat     3000

cctaacgatg taacctttac ttactcacac ttcgctgtac ctcaagcgaa aggaaacaat     3060

gtcgtgatta caagctatat gacaaacaga ggattctacg cagacaaaca atcaacgttt     3120

gcgccaagct tcctgctgaa catcaaaggc aagaaaacat ctgttgtcaa agacagcatc     3180

cttgaacaag gacaattaac agttaacaaa taaaaacgca aaagaaaatg ccgatattga     3240

ctaccggaag cagtgtgacc gtgtgcttct caaatgcctg attcaggctg tctatgtgtg     3300

actgttgagc tgtaacaagt tgtctcaggt gttcaatttc atgttctagt tgctttgttt     3360

tactggtttc acctgttcta ttaggtgtta catgctgttc atctgttaca ttgtcgatct     3420

gttcatggtg aacagcttta aatgcaccaa aaactcgtaa aagctctgat gtatctatct     3480

tttttacacc gttttcatct gtgcatatgg acagttttcc ctttgat                   3527


<210>  53
<211>  2250
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic plasmid

<400>  53
ccatctctac cggttcgctt tcactggata tcgcgcttgg ggcaggtggt ctgccgatgg       60

gccgtatcgt cgaaatctac ggaccggaat cttccggtaa aaccacgctg acgctgcagg      120

tgatcgccgc agcgcagcgt gaaggtaaaa cctgtgcgtt tatcgatgct gaacacgcgc      180

tggacccaat ctacgcacgt aaactgggcg tcgatatcga caacctgctg tgctcccagc      240

cggacaccgg cgagcaggca ctggaaatct gtgacgccct ggcgcgttct ggcgcagtag      300

acgttatcgt cgttgactcc gtggcggcac tgacgccgaa agcggaaatc gaaggcgaaa      360

tcgatgactc tcacatgggc cttgcggcac gtatgatgag ccaggcgatg cgtaagctgg      420

cgggtaacct gaagcagtcc aacacgctgc tgatcttcat caaccagatc cgtatgaaaa      480

ttggtgtgat gttcggtaac ccggaaacca ctaccggtgg taacgcgctg aaattctacg      540

cctctgttcg tctcgacatc cgtcgtatcg gcgcggtgaa agagggcgaa aacgtggtgg      600

gtagcgaaac ccgcgtgaaa gtggtgaaga acaaaatcgc tgcgccgttt aaacaggctg      660

aattccagat cctctacggc gaaggtatca acttctacgg cgaactggtt gacctgggcg      720

taaaagagaa gctgatcgag aaagcaggcg cgtggtacag ctacaaaggt gagaagatcg      780

gtcagggtaa agcgaatgcg actgcctggc tgaaagataa cccggaaacc gcgaaagaga      840

tcgagaagaa agtacgtgag ttgctgctga gcaacccgaa ctcaacgccg gatttctctg      900

tagatgatag cgaaggcgta gcagaaacta acgaagattt ttaatgattg cagtccagtt      960

acgctggagt ctgaggctcg tcctgaatga tatcaagctt gaattcgttg gttcatcccg     1020

tgggcattgc atagggataa cagggtaatc taaatacatt caaatatcta tccgctcatg     1080

agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagaatat gagtattcaa     1140

catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac     1200

ccagaaacgc tggtgaaagt aaaagatgcc gaagatcagt tgggtgcacg tgtgggttac     1260

atcgaactgg acctcaacag cggtaagatt cttgagagtt ttcgccccga agaacgtttc     1320

ccaatgatga gcacttttaa agttctgctc tgtggcgcgg tattatcccg tattgacgcc     1380

gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca     1440

ccagtcacag aaaagcatct tacggacggc atgacagtac gcgaattatg cagcgctgcc     1500

ataaccatga gtgataacac ggcggccaac ttacttctga caacgatcgg aggaccgaag     1560

gagcttaccg cttttttgca caacatgggt gatcatgtaa ctcgccttga tcgttgggaa     1620

ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagctatg     1680

gcaacaacgt tgcgcaaact cttaactggc gaacttctta ctctcgcttc ccggcaacaa     1740

ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg     1800

gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtcccg cggtattatt     1860

gcagccctgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagc     1920

caggcaacta tggacgaacg taatcgccag atcgctgaga taggtgcctc cctgattaag     1980

cattggtaat aaccaggcat ctcgtcttgt ttgatacaca agggtcgcat ctgcggccct     2040

tttgcttttt taagttgtaa ggatatgcca tgacagaatc aacatcccgt cgcccggcat     2100

atgctcgcct gttggatcgt gcggtacgca ttctggcggt gcgcgatcac agtgagcaag     2160

aactgcgacg taaactcgcg gcaccgatta tgggcaaaaa tggcccagaa gagattgatg     2220

ctacggcaga agattacgag cgcgttattg                                      2250


<210>  54
<211>  1056
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic plasmid

<400>  54
tgattgcagt ccagttacgc tggagtctga ggctcgtcct gaatgatatc aagcttgaat       60

tcgttggttc atcccgtggg cattgcatag ggataacagg gtaatctaaa tacattcaaa      120

tatctatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa      180

gaatatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct      240

tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgccgaag atcagttggg      300

tgcacgtgtg ggttacatcg aactggacct caacagcggt aagattcttg agagttttcg      360

ccccgaagaa cgtttcccaa tgatgagcac ttttaaagtt ctgctctgtg gcgcggtatt      420

atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga      480

cttggttgag tactcaccag tcacagaaaa gcatcttacg gacggcatga cagtacgcga      540

attatgcagc gctgccataa ccatgagtga taacacggcg gccaacttac ttctgacaac      600

gatcggagga ccgaaggagc ttaccgcttt tttgcacaac atgggtgatc atgtaactcg      660

ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac      720

gatgcctgta gctatggcaa caacgttgcg caaactctta actggcgaac ttcttactct      780

cgcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct      840

gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg      900

gtcccgcggt attattgcag ccctggggcc agatggtaag ccctcccgta tcgtagttat      960

ctacacgacg gggagccagg caactatgga cgaacgtaat cgccagatcg ctgagatagg     1020

tgcctccctg attaagcatt ggtaataacc aggcat                               1056


<210>  55
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence

<400>  55
gatttccgtg gcgcagaaaa aagcaaatgg cacatctgtt tgggtataat cgcgcccatg       60

ctttttcgcc atgattgcag tccagttacg                                        90


<210>  56
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence

<400>  56
gggtgaaatc aatcttccat tcgcctttta atgagttcag gtttttcagg cgcaggctga       60

gaattatgcc tggttattac caatgcttaa                                        90


<210>  57
<211>  1000
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic plasmid

<400>  57
gatcatttca cgaatttttg cggatacatc acctacatat acccctgcac gtacctccaa       60

caaccagatg gctaatctgc ctcgtaagcg cggaggtaca ttttcagtga ccacgaccaa      120

catactcatt tcctgacgga tggccttttt gcgtttctac aaactctttt tgtttatttt      180

tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat      240

aatattgaaa aaggaagagt atgactgaat acaagcccac ggtacgcttg gcgacgcgcg      300

acgatgttcc ccgcgctgtt cgtacattag ctgcggcctt tgcagattac ccagcgacgc      360

gccatacggt cgatccggac cgccatatcg agcgtgtcac agaattgcag gaacttttct      420

taactcgcgt gggccttgac atcggaaagg tctgggtggc tgacgatggc gctgcagtgg      480

ctgtttggac cactccggag agtgtagagg ctggtgcagt gttcgccgaa attggtcctc      540

gtatggccga attaagtgga agtcgtctgg cagcccaaca acaaatggaa gggttgcttg      600

cgccccaccg tccgaaagaa cccgcgtggt tccttgccac cgttggagta agcccagatc      660

accaggggaa gggtttagga tctgccgtag ttttaccagg tgtggaggca gcagaacgtg      720

cgggagttcc ggccttcctt gagacgtcgg cgccgcgcaa tttaccgttt tacgaacgtc      780

ttggattcac cgttacggcg gacgtggagg tgccggaggg accccgtact tggtgtatga      840

ctcgtaaacc gggagcctga taatttatta cacctcaatc acagtggagc caaagatagc      900

aagccacatc ccatcgattt agctggccca ataccttgct gtacaagatc tattaacgct      960

ggcgcgtcgt tgatggtgag cacaccttca aagcaaaccg                           1000


<210>  58
<211>  1000
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic plasmid

<400>  58
gcggtttatc cccgctggcg cggggaactc tctaaaagta tacatttgtt cttaaagcat       60

tttttcccat aaaaacaacc caccaacctt aatgtaacat ttccttatta ttaaagatca      120

gctaattctt tgttttcctg acggatggcc tttttgcgtt tctacaaact ctttttgttt      180

atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct      240

tcaataatat tgaaaaagga agagtatgac tgaatacaag cccacggtac gcttggcgac      300

gcgcgacgat gttccccgcg ctgttcgtac attagctgcg gcctttgcag attacccagc      360

gacgcgccat acggtcgatc cggaccgcca tatcgagcgt gtcacagaat tgcaggaact      420

tttcttaact cgcgtgggcc ttgacatcgg aaaggtctgg gtggctgacg atggcgctgc      480

agtggctgtt tggaccactc cggagagtgt agaggctggt gcagtgttcg ccgaaattgg      540

tcctcgtatg gccgaattaa gtggaagtcg tctggcagcc caacaacaaa tggaagggtt      600

gcttgcgccc caccgtccga aagaacccgc gtggttcctt gccaccgttg gagtaagccc      660

agatcaccag gggaagggtt taggatctgc cgtagtttta ccaggtgtgg aggcagcaga      720

acgtgcggga gttccggcct tccttgagac gtcggcgccg cgcaatttac cgttttacga      780

acgtcttgga ttcaccgtta cggcggacgt ggaggtgccg gagggacccc gtacttggtg      840

tatgactcgt aaaccgggag cctgataatt cagctactcc gatggcctgc atctcccagt      900

gaaacaggaa gcggaatggc aacaggctgt gcatcttcag gtggggccgg cggttgtatt      960

tctccagcgg caagcacgtc ctctataagc ggaatcaatt                           1000


<210>  59
<211>  1496
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic plasmid

<400>  59
tatgagcagc atcgaaaaat agcccgctga tatcatcgat aatactaaaa aaacagggag       60

gctattacca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt      120

atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg      180

cctttctgcg tttatatctt tctgacacct tactatctta caaatgtaac aaaaaagtta      240

tttttctgta attcgagcat gtcatgttac cccgcgagca taaaacgcgt gtgtaggagg      300

ataatctatg gatttgtcac agctaacacc acgtcgtccc tatctgctgc gtgcattcta      360

tgagtggttg ctggataacc agctcacgcc gcacctggtg gtggatgtga cgctccctgg      420

cgtgcaggtt cctatggaat atgcgcgtga cgggcaaatc gtactcaaca ttgcgccgcg      480

tgctgtcggc aatctggaac tggcgaatga tgaggtgcgc tttaacgcgc gctttggtgg      540

cattccgcgt caggtttctg tgccgctggc tgccgtgctg gctatctacg cccgtgaaaa      600

tggcgcaggc acgatgtttg agcctgaagc tgcctacgat gaagatacca gcatcatgaa      660

tgatgaagag gcatcggcag acaacgaaac cgttatgtcg gttattgatg gcgacaagcc      720

agatcacgat gatgacactc atcctgacga tgaacctccg cagccaccac gcggtggtcg      780

accggcatta cgcgttgtga agtaatcgac ctagcataac cccgcggggc ctcttcgggg      840

gtctcgcggg gttttttgct gaaagaagct tcaaataaaa cgaaaggctc agtcgaaaga      900

ctgggccttt cgttttatct gttgtttgtc gctgcggccg ggtcaggtat gatttaaatg      960

gtcagtaacg ggtcttgagg ggttttttgc cacagctaac accacgtcgt ccctatctgc     1020

tgccctaggt ctatgagtgg ttgctggata acgtgcgtaa ttgtgctgat ctcttatata     1080

gctgctctca ttatctctct accctgaagt gactctctca cctgtaaaaa taatatctca     1140

caggcttaat agtttcttaa tacaaagcct gtaaaacgtc aggataactt ctatattcag     1200

ggagaccaca acggtttccc tctacaaata attttgttta acttttgaag gagaacaaat     1260

gaatttgctt attgataact ggatccctgt acgcccgcga aacgggggga aagtccaaat     1320

cataaatctg caatcgctat actgcagtag agatcagtgg cgattaagtt tgccccgtga     1380

cgatatggaa ctggccgctt tagcactgct ggtttgcatt gggcaaatta tcgccccggc     1440

aaaagatgac gttgaatttc gacatcgcat aatgaatccg ctcactgaag atgagt         1496


<210>  60
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  60
gggagaccac aacgg                                                        15


<210>  61
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  61
cgcagtcgaa cgaccg                                                       16


