                         SEQUENCE LISTING

<110>  Doublerainbow Biosciences Inc.
 
<120>  Ivacaftor Glycosides, Methods Of Making, And Uses Thereof In 
       Treating Cystic Fibrosis

<130>  5767.1000001

<150>  US 62/990,101
<151>  2020-03-16

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  392
<212>  PRT
<213>  Bacillus subtilis

<400>  1

Met Lys Lys Tyr His Ile Ser Met Ile Asn Ile Pro Ala Tyr Gly His 
1               5                   10                  15      


Val Asn Pro Thr Leu Ala Leu Val Glu Lys Leu Cys Glu Lys Gly His 
            20                  25                  30          


Arg Val Thr Tyr Ala Thr Thr Glu Glu Phe Ala Pro Ala Val Gln Gln 
        35                  40                  45              


Ala Gly Gly Glu Ala Leu Ile Tyr His Thr Ser Leu Asn Ile Asp Pro 
    50                  55                  60                  


Lys Gln Ile Arg Glu Met Met Glu Lys Asn Asp Ala Pro Leu Ser Leu 
65                  70                  75                  80  


Leu Lys Glu Ser Leu Ser Ile Leu Pro Gln Leu Glu Glu Leu Tyr Lys 
                85                  90                  95      


Asp Asp Gln Pro Asp Leu Ile Ile Tyr Asp Phe Val Ala Leu Ala Gly 
            100                 105                 110         


Lys Leu Phe Ala Glu Lys Leu Asn Val Pro Val Ile Lys Leu Cys Ser 
        115                 120                 125             


Ser Tyr Ala Gln Asn Glu Ser Phe Gln Leu Gly Asn Glu Asp Met Leu 
    130                 135                 140                 


Lys Lys Ile Arg Glu Ala Glu Ala Glu Phe Lys Ala Tyr Leu Glu Gln 
145                 150                 155                 160 


Glu Lys Leu Pro Ala Val Ser Phe Glu Gln Leu Ala Val Pro Glu Ala 
                165                 170                 175     


Leu Asn Ile Val Phe Met Pro Lys Ser Phe Gln Ile Gln His Glu Thr 
            180                 185                 190         


Phe Asp Asp Arg Phe Cys Phe Val Gly Pro Ser Leu Gly Glu Arg Lys 
        195                 200                 205             


Glu Lys Glu Ser Leu Leu Ile Asp Lys Asp Asp Arg Pro Leu Met Leu 
    210                 215                 220                 


Ile Ser Leu Gly Thr Ala Phe Asn Ala Trp Pro Glu Phe Tyr Lys Met 
225                 230                 235                 240 


Cys Ile Lys Ala Phe Arg Asp Ser Ser Trp Gln Val Ile Met Ser Val 
                245                 250                 255     


Gly Lys Thr Ile Asp Pro Glu Ser Leu Glu Asp Ile Pro Ala Asn Phe 
            260                 265                 270         


Thr Ile Arg Gln Ser Val Pro Gln Leu Glu Val Leu Glu Lys Ala Asp 
        275                 280                 285             


Leu Phe Ile Ser His Gly Gly Met Asn Ser Thr Met Glu Ala Met Asn 
    290                 295                 300                 


Ala Gly Val Pro Leu Val Val Ile Pro Gln Met Tyr Glu Gln Glu Leu 
305                 310                 315                 320 


Thr Ala Asn Arg Val Asp Glu Leu Gly Leu Gly Val Tyr Leu Pro Lys 
                325                 330                 335     


Glu Glu Val Thr Val Ser Ser Leu Gln Glu Ala Val Gln Ala Val Ser 
            340                 345                 350         


Ser Asp Gln Glu Leu Leu Ser Arg Val Lys Asn Met Gln Lys Asp Val 
        355                 360                 365             


Lys Glu Ala Gly Gly Ala Glu Arg Ala Ala Ala Glu Ile Glu Ala Phe 
    370                 375                 380                 


Met Lys Lys Ser Ala Val Pro Gln 
385                 390         


<210>  2
<211>  400
<212>  PRT
<213>  Bacillus cereus

<400>  2

Met Ala Asn Val Leu Val Ile Asn Phe Pro Gly Glu Gly His Ile Asn 
1               5                   10                  15      


Pro Thr Leu Ala Ile Val Ser Glu Leu Ile Arg Arg Gly Glu Thr Val 
            20                  25                  30          


Val Ser Tyr Cys Ile Glu Asp Tyr Arg Lys Lys Ile Glu Ala Thr Gly 
        35                  40                  45              


Ala Gln Phe Arg Val Phe Glu Asn Phe Leu Ser Gln Ile Asn Ile Met 
    50                  55                  60                  


Glu Arg Val Asn Glu Gly Gly Ser Pro Leu Thr Met Leu Ser His Met 
65                  70                  75                  80  


Met Glu Ala Ser Glu Arg Ile Val Thr Gln Ile Val Glu Glu Thr Lys 
                85                  90                  95      


Gly Glu Lys Tyr Asp Tyr Leu Ile Tyr Asp Asn His Phe Pro Val Gly 
            100                 105                 110         


Arg Ile Ile Ala Asn Val Leu Lys Leu Pro Ser Val Ser Ser Cys Thr 
        115                 120                 125             


Thr Phe Ala Phe Asn Gln Tyr Ile Thr Phe Asn Asp Glu His Glu Ser 
    130                 135                 140                 


Arg Glu Val Asp Glu Thr Asn Pro Leu Tyr Gln Ser Cys Leu Ala Gly 
145                 150                 155                 160 


Met Glu Lys Trp Asn Lys Gln Tyr Gly Met Lys Cys Asn Ser Met Tyr 
                165                 170                 175     


Asp Ile Met Asn His Pro Gly Asp Ile Thr Ile Val Tyr Thr Ser Lys 
            180                 185                 190         


Glu Tyr Gln Pro Arg Ser Asp Val Phe Asp Glu Ser Tyr Lys Phe Val 
        195                 200                 205             


Gly Pro Ser Ile Ala Thr Arg Lys Glu Val Gly Ser Phe Pro Met Glu 
    210                 215                 220                 


Asp Leu Lys Asp Glu Lys Leu Ile Phe Ile Ser Met Gly Thr Val Phe 
225                 230                 235                 240 


Asn Glu Gln Pro Glu Leu Tyr Glu Lys Cys Phe Glu Ala Phe Lys Asp 
                245                 250                 255     


Val Glu Ala Thr Val Val Leu Val Val Gly Lys Lys Ile Asn Ile Ser 
            260                 265                 270         


Gln Phe Glu Asn Ile Pro Asn Asn Phe Lys Leu Tyr Asn Tyr Val Pro 
        275                 280                 285             


Gln Leu Glu Leu Leu Gln Tyr Ala Asp Val Phe Val Thr His Gly Gly 
    290                 295                 300                 


Met Asn Ser Ser Ser Glu Ala Leu Tyr Tyr Gly Val Pro Leu Val Val 
305                 310                 315                 320 


Ile Pro Val Thr Gly Asp Gln Pro Leu Val Ala Lys Arg Val Asn Glu 
                325                 330                 335     


Val Gly Ala Gly Ile Arg Leu Asn Arg Lys Glu Leu Thr Ser Glu Met 
            340                 345                 350         


Leu Arg Glu Ser Val Lys Lys Val Met Asp Asp Val Thr Phe Lys Glu 
        355                 360                 365             


Lys Ser Arg Lys Val Gly Glu Ser Leu Arg Asn Ala Gly Gly Tyr Asn 
    370                 375                 380                 


Arg Ala Val Asp Glu Ile Leu Lys Met Asn Ser Tyr Ser Lys Leu Lys 
385                 390                 395                 400 


<210>  3
<211>  394
<212>  PRT
<213>  Bacillus methylotrophicus

<400>  3

Met Arg Lys Thr His Ile Ala Ile Ile Asn Val Ala Ala His Gly His 
1               5                   10                  15      


Val Asn Pro Thr Leu Pro Val Ala Glu Glu Leu Val Asn Arg Gly Tyr 
            20                  25                  30          


Lys Val Thr Phe Ala Thr Thr Glu Glu Phe Glu Ala Ser Val Ala Lys 
        35                  40                  45              


Thr Gly Ala Ile Pro Val Leu Tyr Arg Thr Ser Ile Lys Ala Asp Pro 
    50                  55                  60                  


Glu Thr Ile Lys Glu Arg Val Asn Lys Asn Asp Ala Phe Val Met Phe 
65                  70                  75                  80  


Leu Glu Glu Ala Val Glu Val Leu Pro Gln Leu Glu Glu Leu Tyr Lys 
                85                  90                  95      


Asp Asp Leu Pro Asp Ile Val Leu Phe Asp Phe Leu Ala Leu Ala Gly 
            100                 105                 110         


Arg Leu Phe Ala Asp Asn Gly Gly Ile Pro Ala Val Lys Phe Cys Pro 
        115                 120                 125             


Ser Tyr Ala Met Asn Glu Tyr Phe Gln Leu Gly Arg Asp Glu Glu Thr 
    130                 135                 140                 


Leu Glu Ala Ala Lys Gln Ala Ile Glu Glu Tyr Gln Glu Gln Ile Glu 
145                 150                 155                 160 


Asn Glu Gln Leu Lys Arg Met Thr Met Glu Glu Phe Phe Met Pro Glu 
                165                 170                 175     


Lys Leu Asn Ile Val Phe Met Pro Arg Ala Phe Gln Pro Lys Gln Glu 
            180                 185                 190         


Thr Phe Asp Glu Arg Phe Cys Phe Val Gly Pro Ser Leu Gly Glu Arg 
        195                 200                 205             


Thr Asn Thr Gly Ser Leu Glu Ile Asp Ala Ala Asp Asp Arg Pro Leu 
    210                 215                 220                 


Met Leu Ile Ser Leu Gly Thr Ala Phe Asn Ala Trp Pro Glu Phe Tyr 
225                 230                 235                 240 


Arg Met Cys Ile Asp Ala Phe Arg Asp Ser Asp Trp Arg Val Ile Met 
                245                 250                 255     


Ser Val Gly Thr Thr Ile Asp Pro Glu Ser Phe Ser Asp Val Pro Asp 
            260                 265                 270         


His Phe Ile Ile Arg Gln His Val Pro Gln Leu Asp Val Leu Glu Lys 
        275                 280                 285             


Ala Lys Leu Phe Val Ser His Gly Gly Met Asn Ser Thr Met Glu Ala 
    290                 295                 300                 


Met Asn Ala Gly Val Pro Leu Val Val Val Pro Gln Met His Glu Gln 
305                 310                 315                 320 


Glu Val Thr Ala Lys Arg Val Asp Glu Leu Gly Leu Gly Val His Leu 
                325                 330                 335     


Pro Pro Lys Glu Val Thr Val Ala Arg Leu Gln Lys Asp Val Gln Asn 
            340                 345                 350         


Val Tyr Gly Asp Lys Asn Ile Leu Ser Arg Val Lys Ser Met Gln Gln 
        355                 360                 365             


Lys Ile Glu Glu Thr Gly Gly Pro Lys Gln Ala Val Cys Ala Ile Glu 
    370                 375                 380                 


Glu Phe Leu Lys Thr Ala Ala Val Glu Asn 
385                 390                 


<210>  4
<211>  396
<212>  PRT
<213>  Bacillus licheniformis

<400>  4

Met Gly His Lys His Ile Ala Ile Phe Asn Ile Pro Ala His Gly His 
1               5                   10                  15      


Ile Asn Pro Thr Leu Ala Leu Thr Ala Ser Leu Val Lys Arg Gly Tyr 
            20                  25                  30          


Arg Val Thr Tyr Pro Val Thr Asp Glu Phe Val Lys Ala Val Glu Glu 
        35                  40                  45              


Thr Gly Ala Glu Pro Leu Asn Tyr Arg Ser Thr Leu Asn Ile Asp Pro 
    50                  55                  60                  


Gln Gln Ile Arg Glu Leu Met Lys Asn Lys Lys Asp Met Ser Gln Ala 
65                  70                  75                  80  


Pro Leu Met Phe Ile Lys Glu Met Glu Glu Val Leu Pro Gln Leu Glu 
                85                  90                  95      


Ala Leu Tyr Glu Asn Asp Lys Pro Asp Leu Ile Leu Phe Asp Phe Met 
            100                 105                 110         


Ala Met Ala Gly Lys Leu Leu Ala Glu Lys Phe Gly Ile Glu Ala Val 
        115                 120                 125             


Arg Leu Cys Ser Thr Tyr Ala Gln Asn Glu His Phe Thr Phe Arg Ser 
    130                 135                 140                 


Ile Ser Glu Glu Phe Lys Ile Glu Leu Thr Pro Glu Gln Glu Asp Ala 
145                 150                 155                 160 


Leu Lys Asn Ser Asn Leu Pro Ser Phe Asn Phe Glu Asp Met Phe Glu 
                165                 170                 175     


Pro Ala Lys Leu Asn Ile Val Phe Met Pro Arg Ala Phe Gln Pro Tyr 
            180                 185                 190         


Gly Glu Thr Phe Asp Glu Arg Phe Ser Phe Val Gly Pro Ser Leu Ala 
        195                 200                 205             


Lys Arg Lys Phe Gln Glu Lys Glu Thr Pro Ile Ile Ser Asp Ser Gly 
    210                 215                 220                 


Arg Pro Val Met Leu Ile Ser Leu Gly Thr Ala Phe Asn Ala Trp Pro 
225                 230                 235                 240 


Glu Phe Tyr His Met Cys Ile Glu Ala Phe Arg Asp Thr Lys Trp Gln 
                245                 250                 255     


Val Ile Met Ala Val Gly Thr Thr Ile Asp Pro Glu Ser Phe Asp Asp 
            260                 265                 270         


Ile Pro Glu Asn Phe Ser Ile His Gln Arg Val Pro Gln Leu Glu Ile 
        275                 280                 285             


Leu Lys Lys Ala Glu Leu Phe Ile Thr His Gly Gly Met Asn Ser Thr 
    290                 295                 300                 


Met Glu Gly Leu Asn Ala Gly Val Pro Leu Val Ala Val Pro Gln Met 
305                 310                 315                 320 


Pro Glu Gln Glu Ile Thr Ala Arg Arg Val Glu Glu Leu Gly Leu Gly 
                325                 330                 335     


Lys His Leu Gln Pro Glu Asp Thr Thr Ala Ala Ser Leu Arg Glu Ala 
            340                 345                 350         


Val Ser Gln Thr Asp Gly Asp Pro His Val Leu Lys Arg Ile Gln Asp 
        355                 360                 365             


Met Gln Lys His Ile Lys Gln Ala Gly Gly Ala Glu Lys Ala Ala Asp 
    370                 375                 380                 


Glu Ile Glu Ala Phe Leu Ala Pro Ala Gly Val Lys 
385                 390                 395     


