                         SEQUENCE LISTING

<110>  National University Of Singapore
 
<120>  Modulation Of Cxcr3 Chemokine Signalling For Osteoporosis Therapy

<130>  70681SG2

<160>  35    

<170>  PatentIn version 3.5

<210>  1
<211>  41
<212>  DNA
<213>  Artificial sequence

<220>
<223>  mpeg1.2F

<400>  1
atagcgagcg gatccagaac agtttctggc agaagttagt c                           41


<210>  2
<211>  41
<212>  DNA
<213>  Artificial sequence

<220>
<223>  mpeg1.2R

<400>  2
aatatgcggc cgctgaattc atccatgttc cgcaggttgt c                           41


<210>  3
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  cxcl9lUP

<400>  3
tgatccgctg ctgggataga                                                   20


<210>  4
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  cxcl9lDOWN

<400>  4
ttagtcccaa gggcttgtcc                                                   20


<210>  5
<211>  28
<212>  DNA
<213>  Artificial sequenece

<220>
<223>  cxcr3.2UP

<400>  5
ctaatatggg ttcacacaca tccaccac                                          28


<210>  6
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  cxcr3.2Down

<400>  6
ctcagaacaa ggggctccaa tc                                                22


<210>  7
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  beta-actinUP

<400>  7
gccaacaggg agaagatgac                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  beta-actinDOWN

<400>  8
catcaccaga gtccatgacg                                                   20


<210>  9
<211>  28
<212>  DNA
<213>  Artificial sequence

<220>
<223>  cxcl9lF

<400>  9
gtttagcttg tctcccgggc aggccacc                                          28


<210>  10
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  cxcl9lR

<400>  10
ttgtgaggct gaggagga                                                     18


<210>  11
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  cxcl9l crRNA1

<400>  11
tcagacaata cccggtgtca ggg                                               23


<210>  12
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  cxcl9l crRNA2

<400>  12
tttgaatcca gagcaaccta tgg                                               23


<210>  13
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  cxcr3.2 crRNA1

<400>  13
ccaatgaaga ttggagcccc ttg                                               23


<210>  14
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  cxcr3.2 crRNA2

<400>  14
cctcggttta agcggagtgg aca                                               23


<210>  15
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  cxcr3.2 crRNA3

<400>  15
tctgctgtac ggatttgtcg ggg                                               23


<210>  16
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  cxcl9lgenoF

<400>  16
tctgtatagt gagggaaacg gacgg                                             25


<210>  17
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  cxcl9lgenoR

<400>  17
cctgggcttt ctttttgaac aatgc                                             25


<210>  18
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  cxcr3.2genoF

<400>  18
tgtttgtgca gaaaataggc attcc                                             25


<210>  19
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  cxcr3.2genoR

<400>  19
tcggagcctc tttcctttcc                                                   20


<210>  20
<211>  10
<212>  DNA
<213>  Artificial sequence

<220>
<223>  5'-cxcr3.2 WT DNA


<220>
<221>  misc_feature
<222>  (3)..(5)
<223>  PAM sequence

<220>
<221>  misc_feature
<222>  (3)..(10)
<223>  gRNA1

<400>  20
atccaatgaa                                                              10


<210>  21
<211>  8
<212>  DNA
<213>  Artificial sequence

<220>
<223>  3'-cxcr3.2 WT DNA


<220>
<221>  misc_feature
<222>  (1)..(8)
<223>  gRNA3

<220>
<221>  misc_feature
<222>  (6)..(8)
<223>  PAM sequence

<400>  21
tgtcgggg                                                                 8


<210>  22
<211>  5
<212>  DNA
<213>  Artificial sequence

<220>
<223>  5'-cxcr3.2 mutant DNA


<220>
<221>  misc_feature
<222>  (3)..(5)
<223>  PAM sequence

<220>
<221>  misc_feature
<222>  (3)..(5)
<223>  gRNA1

<400>  22
atcca                                                                    5


<210>  23
<211>  4
<212>  DNA
<213>  Artificial sequence

<220>
<223>  3'-cxcr3.2 mutant DNA


<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  gRNA3

<220>
<221>  misc_feature
<222>  (2)..(4)
<223>  PAM sequence

<400>  23
gggg                                                                     4


<210>  24
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  5'-cxcr3.2 WT predicted amino acid sequence


<220>
<221>  MISC_FEATURE
<222>  (4)..(6)
<223>  gRNA1

<220>
<221>  MISC_FEATURE
<222>  (5)..(6)
<223>  prematurely truncated amino acid sequence

<400>  24

Pro Gly Asp Pro Met Lys 
1               5       


<210>  25
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  mid-cxcr3.2 WT predicted amino acid sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(2)
<223>  prematurely truncated amino acid sequence

<220>
<221>  MISC_FEATURE
<222>  (1)..(3)
<223>  gRNA3

<400>  25

Phe Val Gly Glu Lys 
1               5   


<210>  26
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  3'-cxcr3.2 WT predicted amino acid sequence


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  stop

<400>  26

Val Met Val Xaa 
1               


<210>  27
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  5'-cxcr3.2 mutant predicted amino acid sequence


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  gRNA1

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  gRNA3

<400>  27

Pro Gly Asp Pro Gly Glu Lys 
1               5           


<210>  28
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  3'-cxcr3.2 mutant predicted amino acid sequence


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  stop

<400>  28

Val Met Val Xaa 
1               


<210>  29
<211>  12
<212>  DNA
<213>  Artificial sequence

<220>
<223>  5'-cxcl9l WT DNA


<220>
<221>  misc_feature
<222>  (1)..(12)
<223>  gRNA1

<220>
<221>  misc_feature
<222>  (10)..(12)
<223>  PAM sequence

<400>  29
ccggtgtcag gg                                                           12


<210>  30
<211>  10
<212>  DNA
<213>  Artificial sequence

<220>
<223>  3'-cxcl9l WT DNA


<220>
<221>  misc_feature
<222>  (1)..(10)
<223>  gRNA3

<220>
<221>  misc_feature
<222>  (8)..(10)
<223>  PAM sequence

<400>  30
caacctatgg                                                              10


<210>  31
<211>  6
<212>  DNA
<213>  Artificial sequence

<220>
<223>  5'-cxcl9l mutant DNA


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  gRNA1

<220>
<221>  misc_feature
<222>  (3)..(6)
<223>  deletion

<400>  31
cccaca                                                                   6


<210>  32
<211>  5
<212>  DNA
<213>  Artificial sequence

<220>
<223>  3'-cxcl9l mutant DNA


<220>
<221>  misc_feature
<222>  (1)..(5)
<223>  gRNA3

<220>
<221>  misc_feature
<222>  (3)..(5)
<223>  PAM sequence

<400>  32
tatgg                                                                    5


<210>  33
<211>  10
<212>  PRT
<213>  Artificial sequence

<220>
<223>  5'-cxcl9l WT predicted amino acid sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(6)
<223>  gRNA1

<400>  33

Thr Ile Pro Gly Val Arg Gly Arg Leu Lys 
1               5                   10  


<210>  34
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  3'-cxcl9l WT predicted amino acid sequence


<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  stop

<400>  34

Ser Ser Ala Ser Gln Xaa 
1               5       


<210>  35
<211>  10
<212>  PRT
<213>  Artificial sequence

<220>
<223>  cxcl9l mutant predicted amino acid sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(3)
<223>  gRNA1

<220>
<221>  MISC_FEATURE
<222>  (4)..(10)
<223>  prematurely truncated amino acid sequence

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  stop

<400>  35

Thr Ile Pro His Ile Trp Pro Asn Ser Xaa 
1               5                   10  


