                         SEQUENCE LISTING

<110>  Nanyang Technological University
       Agency for Science, Technology and Research
 
<120>  DIRECT CLEAVAGE OF HUMAN NLRP1 BY ENTEROVIRAL 3C PROTEASE 
       TRIGGERS N-GLYCINE DEGRON-MEDIATED INFLAMMASOME ACTIVATION IN 
       AIRWAY EPITHELIA

<130>  SP102476WO

<150>  SG10202002526P
<151>  2020-03-19

<160>  41    

<170>  PatentIn version 3.5

<210>  1
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NLRP1#1

<400>  1
gatagcccga gtgacatcgg                                                   20


<210>  2
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NLRP1#2

<400>  2
agcccgagtg acatcggtgg                                                   20


<210>  3
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CASP1#1

<400>  3
acagacaagg gtgctgaaca                                                   20


<210>  4
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CASP1#2

<400>  4
attgactccg ttattccgaa                                                   20


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CASP1#3

<400>  5
tgactccgtt attccgaaag                                                   20


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ASC#2

<400>  6
gctaacgtgc tgcgcgacat                                                   20


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ASC#3

<400>  7
catgtcgcgc agcacgttag                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  UBR2#1

<400>  8
tgcataactt gaactttgag                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  UBR2#2

<400>  9
tccatgcaca aaacacaagt                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  UBR2#3

<400>  10
actgtggtga tactgaagcc                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  UBR2#4

<400>  11
tgatactgaa gcctggaaag                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  UBR2#5

<400>  12
ttgcatgctg tttaatgatg                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ZER1#5

<400>  13
cagggaccca atcaatcatg                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ZER1#6

<400>  14
ggcaggacga gtctatccag                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ZER1#7

<400>  15
aggctgaaga agctctcgtg                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ZYG11B#5

<400>  16
agcgctcgta aggatcctcg                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ZYG11B#6

<400>  17
gcagtggctt tgcaaccatg                                                   20


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ZYG11B#7

<400>  18
cttggttaag ttaaatacac                                                   20


<210>  19
<211>  53
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human 3Cpro cleavage site

<400>  19

Leu Met Pro Trp Ile His Glu Leu Pro Ala Gly Cys Thr Gln Gly Ser 
1               5                   10                  15      


Glu Arg Arg Val Leu Arg Gln Leu Pro Asp Thr Ser Gly Arg Arg Trp 
            20                  25                  30          


Arg Glu Ile Ser Ala Ser Leu Leu Tyr Gln Ala Leu Pro Ser Ser Pro 
        35                  40                  45              


Asp His Glu Ser Pro 
    50              


<210>  20
<211>  53
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chimpanzee 3Cpro cleavage site

<400>  20

Leu Met Pro Trp Ile His Glu Leu Pro Ala Gly Cys Thr Gln Gly Ser 
1               5                   10                  15      


Glu Arg Arg Val Leu Arg Gln Leu Pro Asp Thr Ser Gly Arg Arg Trp 
            20                  25                  30          


Arg Glu Ile Ser Ala Ser Leu Leu Tyr Gln Ala Leu Pro Ser Ser Pro 
        35                  40                  45              


Asp His Glu Ser Pro 
    50              


<210>  21
<211>  53
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Bonobo 3Cpro cleavage site

<400>  21

Leu Met Pro Trp Ile His Glu Leu Pro Ala Gly Cys Thr Gln Gly Ser 
1               5                   10                  15      


Glu Arg Arg Val Leu Arg Gln Leu Pro Asp Thr Ser Gly Arg Arg Trp 
            20                  25                  30          


Arg Glu Ile Ser Ala Ser Leu Leu Tyr Gln Ala Leu Pro Ser Ser Pro 
        35                  40                  45              


Asp His Glu Ser Pro 
    50              


<210>  22
<211>  53
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Gorilla 3Cpro cleavage site

<400>  22

Leu Met Pro Trp Ile His Glu Leu Pro Ala Gly Cys Thr Gln Gly Ser 
1               5                   10                  15      


Glu Arg Arg Val Leu Arg Gln Leu Pro Asp Thr Ser Gly Arg Arg Trp 
            20                  25                  30          


Arg Glu Ile Ser Ala Ser Leu Leu Tyr Gln Ala Leu Pro Ser Ser Pro 
        35                  40                  45              


Asp His Glu Ser Pro 
    50              


<210>  23
<211>  53
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Orangutan 3Cpro cleavage site

<400>  23

Leu Met Pro Trp Ile His Glu Leu Pro Ala Gly Cys Thr Gln Gly Ser 
1               5                   10                  15      


Glu Arg Arg Val Leu Arg Gln Leu Pro Asp Thr Ser Gly Arg Arg Trp 
            20                  25                  30          


Arg Glu Ile Ser Ser Ser Leu Leu Tyr Gln Ala Leu Pro Ser Ser Pro 
        35                  40                  45              


Asp Tyr Glu Ser Pro 
    50              


<210>  24
<211>  53
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Gibbon 3Cpro cleavage site

<400>  24

Leu Met Pro Trp Ile Cys Glu Leu Pro Ala Gly Cys Thr Gln Gly Ser 
1               5                   10                  15      


Glu Arg Arg Val Leu Arg Gln Leu Pro Asp Thr Ser Gly Arg Arg Trp 
            20                  25                  30          


Arg Glu Ile Ser Ser Ser Leu Leu Tyr Gln Ala Leu Pro Ser Ser Pro 
        35                  40                  45              


Asp His Glu Ser Pro 
    50              


<210>  25
<211>  53
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Olive Baboon 3Cpro cleavage site

<400>  25

Leu Arg Pro Trp Asn Arg Glu Leu Pro Gly Glu Cys Thr Gln Gly Ser 
1               5                   10                  15      


Glu Arg Arg Val Leu Arg Gln Leu Pro Asp Thr Ser Gly Arg Arg Trp 
            20                  25                  30          


Arg Glu Ile Ser Ser Ser Leu Leu Tyr Gln Ala Phe Pro Ser Ser Pro 
        35                  40                  45              


Asp Phe Glu Ser Pro 
    50              


<210>  26
<211>  53
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Gelada 3Cpro cleavage site

<400>  26

Leu Arg Pro Trp Asn Arg Glu Leu Pro Ala Glu Cys Thr Gln Gly Ser 
1               5                   10                  15      


Glu Arg Arg Val Leu Arg Gln Leu Pro Asp Thr Ser Gly Arg Arg Trp 
            20                  25                  30          


Arg Glu Ile Ser Ser Ser Leu Leu Tyr Gln Ala Leu Pro Ser Ser Pro 
        35                  40                  45              


Asp Phe Glu Ser Pro 
    50              


<210>  27
<211>  53
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sooty Mangabey 3Cpro cleavage site

<400>  27

Leu Arg Pro Trp Asn Arg Glu Leu Pro Ala Glu Cys Thr Gln Gly Ser 
1               5                   10                  15      


Glu Arg Arg Val Leu Arg Gln Leu Pro Asp Thr Ser Gly His Arg Trp 
            20                  25                  30          


Arg Glu Ile Ser Ser Ser Leu Leu Tyr Gln Ala Phe Pro Ser Ser Pro 
        35                  40                  45              


Asp Phe Glu Ser Pro 
    50              


<210>  28
<211>  53
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Drill 3Cpro cleavage site

<400>  28

Leu Arg Pro Trp Asn Arg Glu Leu Pro Ala Glu Cys Thr Gln Gly Ser 
1               5                   10                  15      


Glu Arg Arg Val Leu Arg Gln Leu Pro Asp Thr Ser Gly Arg Arg Trp 
            20                  25                  30          


Arg Glu Ile Ser Ser Ser Leu Leu Tyr Gln Ala Phe Pro Ser Ser Pro 
        35                  40                  45              


Asp Phe Glu Ser Pro 
    50              


<210>  29
<211>  53
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Macaque 3Cpro cleavage site

<400>  29

Leu Arg Pro Trp Asn Arg Glu Leu Pro Ala Glu Cys Thr Gln Gly Ser 
1               5                   10                  15      


Glu Arg Arg Val Leu Arg Gln Leu Pro Asp Thr Ser Gly Arg Arg Trp 
            20                  25                  30          


Arg Glu Ile Ser Ser Ser Leu Leu Tyr Gln Ala Leu Pro Ser Ser Pro 
        35                  40                  45              


Asp Phe Glu Ser Pro 
    50              


<210>  30
<211>  53
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Pig-tailed Macaque 3Cpro cleavage site

<400>  30

Leu Arg Pro Trp Asn Arg Glu Leu Pro Ala Glu Cys Thr Gln Gly Ser 
1               5                   10                  15      


Glu Arg Arg Val Leu Arg Gln Leu Pro Asp Thr Ser Gly Arg Arg Trp 
            20                  25                  30          


Arg Glu Ile Ser Ser Ser Leu Leu Tyr Gln Val Leu Pro Ser Ser Pro 
        35                  40                  45              


Asp Phe Glu Ser Pro 
    50              


<210>  31
<211>  53
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Vervet-AGM 3Cpro cleavage site

<400>  31

Leu Arg Pro Trp Asn Arg Glu Leu Pro Ala Glu Cys Thr Gln Gly Ser 
1               5                   10                  15      


Glu Arg Arg Val Leu Arg Gln Leu Pro Asp Thr Ser Gly Arg Arg Trp 
            20                  25                  30          


Arg Glu Ile Ser Ser Ser Phe Leu Tyr Gln Ala Leu Pro Ser Ser Pro 
        35                  40                  45              


Asp Leu Glu Ser Pro 
    50              


<210>  32
<211>  53
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Black Snub-nosed Monkey 3Cpro cleavage sit

<400>  32

Leu Arg Pro Trp Asn Cys Asp Leu Pro Ala Glu Cys Thr Gln Gly Ser 
1               5                   10                  15      


Glu Arg Arg Val Leu Arg Gln Leu Pro Asp Thr Ser Gly His Arg Trp 
            20                  25                  30          


Arg Glu Ile Ser Ser Ser Leu Leu Tyr Gln Ala Leu Pro Ser Ser Pro 
        35                  40                  45              


Asp Phe Glu Ser Pro 
    50              


<210>  33
<211>  53
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Golden Snub-nosed Monkey 3Cpro cleavage sit

<400>  33

Leu Arg Pro Trp Asn Arg Asp Leu Pro Ala Glu Cys Thr Gln Gly Ser 
1               5                   10                  15      


Glu Arg Arg Val Leu Arg Gln Leu Pro Asp Thr Ser Gly His Arg Trp 
            20                  25                  30          


Arg Glu Ile Ser Ser Ser Leu Leu Tyr Gln Ala Leu Pro Ser Ser Pro 
        35                  40                  45              


Asp Phe Glu Ser Pro 
    50              


<210>  34
<211>  53
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Angola Colobus 3Cpro cleavage site

<400>  34

Leu Arg Ser Trp Asn His Glu Leu Pro Ala Glu Cys Thr Gln Val Ser 
1               5                   10                  15      


Glu Arg Arg Val Leu Arg Gln Leu Pro Asp Thr Ser Gly His Arg Trp 
            20                  25                  30          


Arg Glu Ile Ser Ser Ser Leu Leu Tyr Gln Ala Leu Pro Ser Ser Pro 
        35                  40                  45              


Asp Phe Glu Ser Pro 
    50              


<210>  35
<211>  52
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Capuchin 3Cpro cleavage site

<400>  35

Leu Arg Ser Trp Ile Pro Glu Leu Gln Gly Cys Thr Gln Gly Ser Glu 
1               5                   10                  15      


Arg Ser Ile Leu Arg His Leu Pro Asp Thr Ser Gly His His Trp Arg 
            20                  25                  30          


Glu Ile Thr Ser Ser Leu Leu Tyr Gln Ala Pro Leu Ser Ser Pro Asp 
        35                  40                  45              


Tyr Gly Ser Pro 
    50          


<210>  36
<211>  52
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Bolivian Squirrel Monkey 3Cpro cleavage site

<400>  36

Leu Arg Ser Trp Ile Pro Glu Leu Gln Gly Cys Thr Gln Gly Ser Glu 
1               5                   10                  15      


Arg Ser Val Leu Arg His Leu Pro Asp Met Ser Gly His Arg Trp Arg 
            20                  25                  30          


Glu Ile Thr Ser Ser Leu Leu Tyr Gln Ala Pro Leu Ser Ser Pro Asp 
        35                  40                  45              


Tyr Gly Ser Pro 
    50          


<210>  37
<211>  52
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Marmoset 3Cpro cleavage site

<400>  37

Leu Arg Ser Gln Ile Pro Glu Leu His Gly Cys Thr Gln Arg Ser Glu 
1               5                   10                  15      


Arg Ser Val Ser Arg Arg Leu Pro Asp Thr Ser Gly His Gln Trp Arg 
            20                  25                  30          


Glu Ile Thr Ser Ser Leu Leu Tyr Gln Ala Pro Leu Ser Ser Pro Asp 
        35                  40                  45              


Tyr Gly Ser Pro 
    50          


<210>  38
<211>  52
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ma's Night Monkey 3Cpro cleavage site

<400>  38

Leu Arg Ser Ser Ile Pro Glu Leu Gln Gly Cys Thr Gln Gly Ser Lys 
1               5                   10                  15      


Arg Ser Ile Leu Arg His Leu Pro Asp Thr Ser Gly His Arg Trp Arg 
            20                  25                  30          


Glu Ile Thr Ser Ser Leu Leu Tyr Gln Ala Pro Leu Ser Ser Pro Asp 
        35                  40                  45              


Tyr Gly Ser Pro 
    50          


<210>  39
<211>  53
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Tarsier 3Cpro cleavage site

<400>  39

Leu Gly Ile Leu Gly Pro Gly Ser Leu Glu Ser Pro Gln Asp Ser Glu 
1               5                   10                  15      


Lys Arg Arg Ile Leu Arg Trp Pro Pro Asp Thr Ser Gly Asn Asn Trp 
            20                  25                  30          


Arg Glu Ile Lys Pro Leu Pro Leu Phe Glu Ala Leu Ser Ser Phe Pro 
        35                  40                  45              


Val Tyr Gln Ser Pro 
    50              


<210>  40
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mouse Lemur 3Cpro cleavage site

<400>  40

Ser Ser Pro Asp Phe Pro Cys Phe Gly Leu Cys His Pro Glu Ser Pro 
1               5                   10                  15      


Arg Leu Gln 
            


<210>  41
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Coquerel's Sifaka 3Cpro cleavage site

<400>  41

Pro Pro Pro Leu Leu Pro Trp Gly Thr Pro Arg Ser Gln Lys Arg Glu 
1               5                   10                  15      


Gly Phe Gln 
            


