                         SEQUENCE LISTING

<110>  Eutilex Co., Ltd.
 
<120>  GPC3 CAR- T CELLS SECRETING IL-18 AND METHODS OF MAKING AND USING
        THE SAME

<130>  47683-0041WO1

<150>  62/991,497
<151>  2020-03-18

<150>  63/004,912
<151>  2020-04-03

<150>  63/043,401
<151>  2020-06-24

<160>  22    

<170>  PatentIn version 3.5

<210>  1
<211>  16
<212>  PRT
<213>  Artificial

<220>
<223>  Light chain CDR1

<400>  1

Arg Ser Ser Gln Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His 
1               5                   10                  15      


<210>  2
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Light chain CDR2

<400>  2

Lys Val Ser Asn Arg Phe Ser 
1               5           


<210>  3
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  Light chain CDR3

<400>  3

Ser Gln Asn Thr His Val Pro Pro Thr 
1               5                   


<210>  4
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  Heavy chain CDR1

<400>  4

Asp Tyr Glu Met His 
1               5   


<210>  5
<211>  17
<212>  PRT
<213>  Artificial

<220>
<223>  Heavy chain CDR2

<400>  5

Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  6
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  Heavy chain CDR3

<400>  6

Phe Tyr Ser Tyr Thr Tyr 
1               5       


<210>  7
<211>  345
<212>  DNA
<213>  Artificial

<220>
<223>  Heavy chain variable region

<400>  7
caagtgcaac tcgtacaatc aggtgctgaa gtcaaaaagc cgggagcctc tgttaaagtg       60

tcctgtaaag ccagcggcta cacctttacc gattatgaga tgcactgggt tcggcaggct      120

ccgggccaag gtctcgagtg gatcggggct cttgacccaa agacgggcga cacggcttat      180

tcacaaaaat tcaaaggtag ggctactctg actgccgata agtccaccag caccgcgtat      240

atggagctct ctagcttgcg aagcgaggac acggcggtgt actattgcac acgcttctat      300

agttacacat attggggtca aggcacgctt gtgaccgtgt ctagc                      345


<210>  8
<211>  115
<212>  PRT
<213>  Artificial

<220>
<223>  Heavy chain variable region

<400>  8

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Glu Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe 
    50                  55                  60                  


Lys Gly Arg Ala Thr Leu Thr Ala Asp Lys Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ser 
        115 


<210>  9
<211>  336
<212>  DNA
<213>  Artificial

<220>
<223>  Light chain variable region

<400>  9
gacgtcgtta tgacacagag tcccctctcc ttgccggtga ccctgggtca gcctgcgtcc       60

atctcttgca gatcctccca gtctctggta cactccaacg gcaacacata cttgcactgg      120

taccaacaaa gacctggtca gtcaccgcga cttctcatat ataaagtttc caataggttc      180

agtggagtgc cagacaggtt cagtggttca ggatcaggca ctgatttcac gcttaaaatc      240

agtcgggttg aggcggagga cgtaggagtt tactattgca gccagaatac gcacgtgccg      300

cctacttttg gctctggaac caagttggaa ataaag                                336


<210>  10
<211>  112
<212>  PRT
<213>  Artificial

<220>
<223>  Light chain variable region

<400>  10

Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Leu Gly 
1               5                   10                  15      


Gln Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu His Trp Tyr Gln Gln Arg Pro Gly Gln Ser 
        35                  40                  45              


Pro Arg Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Ser Gln Asn 
                85                  90                  95      


Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


<210>  11
<211>  474
<212>  DNA
<213>  Homo sapiens

<400>  11
tactttggca agcttgaatc taaattatca gtcataagaa atttgaatga ccaagttctc       60

ttcattgacc aaggaaatcg gcctctattt gaagatatga ctgattctga ctgtagagat      120

aatgcacccc ggaccatatt tattataagt atgtataaag atagccagcc tagaggtatg      180

gctgtaacta tctctgtgaa gtgtgagaaa atttcaactc tctcctgtga gaacaaaatt      240

atttccttta aggaaatgaa tcctcctgat aacatcaagg atacaaaaag tgacatcata      300

ttctttcaga gaagtgtccc aggacatgat aataagatgc aatttgaatc ttcatcatac      360

gaaggatact ttctagcttg tgaaaaagag agagaccttt ttaaactcat tttgaaaaaa      420

gaggatgaat tgggggatag atctataatg ttcactgttc aaaacgaaga ctag            474


<210>  12
<211>  474
<212>  DNA
<213>  Homo sapiens

<400>  12
ctagtcttcg ttttgaacag tgaacattat agatctatcc cccaattcat cctctttttt       60

caaaatgagt ttaaaaaggt ctctctcttt ttcacaagct agaaagtatc cttcgtatga      120

tgaagattca aattgcatct tattatcatg tcctgggaca cttctctgaa agaatatgat      180

gtcacttttt gtatccttga tgttatcagg aggattcatt tccttaaagg aaataatttt      240

gttctcacag gagagagttg aaattttctc acacttcaca gagatagtta cagccatacc      300

tctaggctgg ctatctttat acatacttat aataaatatg gtccggggtg cattatctct      360

acagtcagaa tcagtcatat cttcaaatag aggccgattt ccttggtcaa tgaagagaac      420

ttggtcattc aaatttctta tgactgataa tttagattca agcttgccaa agta            474


<210>  13
<211>  60
<212>  DNA
<213>  Homo sapiens

<400>  13
atgtacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacaaacagt       60


<210>  14
<211>  60
<212>  DNA
<213>  Homo sapiens

<400>  14
actgtttgtg acaagtgcaa gacttagtgc aatgcaagac aggagttgca tcctgtacat       60


<210>  15
<211>  726
<212>  DNA
<213>  Artificial

<220>
<223>  huGC33 VHVL

<400>  15
caagtgcaac tcgtacaatc aggtgctgaa gtcaaaaagc cgggagcctc tgttaaagtg       60

tcctgtaaag ccagcggcta cacctttacc gattatgaga tgcactgggt tcggcaggct      120

ccgggccaag gtctggagtg gatcggggct cttgacccaa agacgggcga cacggcttat      180

tcacaaaaat tcaaaggtag ggctactctg actgccgata agtccaccag caccgcgtat      240

atggagctct ctagcttgcg aagcgaggac acggcggtgt actattgcac acgcttctat      300

agttacacat attggggtca aggcacgctt gtgaccgtgt ctagcggtgg cggcggaagt      360

ggtggtggtg gttctggggg cgggggttcc gacgtcgtta tgacacagag tcccctctcc      420

ttgccggtga ccctgggtca gcctgcgtcc atctcttgca gatcctccca gtctctggta      480

cactccaacg gcaacacata cttgcactgg taccaacaaa gacctggtca gtcaccgcga      540

cttctcatat ataaagtttc caataggttc agtggagtgc cagacaggtt cagtggttca      600

ggatcaggca ctgatttcac gcttaaaatc agtcgggttg aggcggagga cgtaggagtt      660

tactattgca gccagaatac gcacgtgccg cctacttttg gctctggaac caagttggaa      720

ataaag                                                                 726


<210>  16
<211>  15
<212>  DNA
<213>  Artificial

<220>
<223>  additional 5 amino acids

<400>  16
cgtttctctg ttgtt                                                        15


<210>  17
<211>  126
<212>  DNA
<213>  Artificial

<220>
<223>  4-1BB intracellular domain

<400>  17
aaacggggca gaaagaaact cctgtatata ttcaaacaac catttatgag accagtacaa       60

actactcaag aggaagatgg ctgtagctgc cgatttccag aagaagaaga aggaggatgt      120

gaactg                                                                 126


<210>  18
<211>  141
<212>  DNA
<213>  Artificial

<220>
<223>  4-1BB intracellular domain with five additional amino acids 
       (euBBz)

<400>  18
cgtttctctg ttgttaaacg gggcagaaag aaactcctgt atatattcaa acaaccattt       60

atgagaccag tacaaactac tcaagaggaa gatggctgta gctgccgatt tccagaagaa      120

gaagaaggag gatgtgaact g                                                141


<210>  19
<211>  339
<212>  DNA
<213>  Artificial

<220>
<223>  CD3-zeta

<400>  19
agagtgaagt tcagcaggag cgcagacgcc cccgcgtaca agcagggcca gaaccagctc       60

tataacgagc tcaatctagg acgaagagag gagtacgatg ttttggacaa gagacgtggc      120

cgggaccctg agatgggggg aaagccgaga aggaagaacc ctcaggaagg cctgtacaat      180

gaactgcaga aagataagat ggcggaggcc tacagtgaga ttgggatgaa aggcgagcgc      240

cggaggggca aggggcacga tggcctttac cagggtctca gtacagccac caaggacacc      300

tacgacgccc ttcacatgca ggccctgccc cctcgctaa                             339


<210>  20
<211>  66
<212>  DNA
<213>  Artificial

<220>
<223>  P2A

<400>  20
ggcagcggcg ccacaaactt ctctctgcta aagcaagcag gtgatgttga agaaaacccc       60

gggcct                                                                  66


<210>  21
<211>  301
<212>  DNA
<213>  Artificial

<220>
<223>  NFAT

<400>  21
acgccttctg tatgaaacag tttttcctcc acgccttctg tatgaaacag tttttcctcc       60

acgccttctg tatgaaacag tttttcctcc acgccttctg tatgaaacag tttttcctcc      120

acgccttctg tatgaaacag tttttcctcc acgccttctg tatgaaacag tttttcctcc      180

tcgaggacat tttgacaccc ccataatatt tttccagaat taacagtata aattgcatct      240

cttgttcaag agttccctat cactctcttt aatcactact cacagtaacc tcaactcctg      300

c                                                                      301


<210>  22
<211>  237
<212>  DNA
<213>  Artificial

<220>
<223>  poly A

<400>  22
cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa       60

aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca      120

ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt      180

gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatc         237


