                         SEQUENCE LISTING

<110>  IPSEN BIOPHARM LIMITED
 
<120>  TREATMENT OF LIMB SPASTICITY

<130>  P66414WO

<150>  GB2003803.0
<151>  2020-03-16

<150>  GB2003867.5
<151>  2020-03-17

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  3888
<212>  DNA
<213>  Clostridium botulinum

<400>  1
atgccattcg tcaacaagca attcaactac aaagacccag tcaacggcgt cgacatcgca       60

tacatcaaga ttccgaacgc cggtcaaatg cagccggtta aggcttttaa gatccacaac      120

aagatttggg ttatcccgga gcgtgacacc ttcacgaacc cggaagaagg cgatctgaac      180

ccgccaccgg aagcgaagca agtccctgtc agctactacg attcgacgta cctgagcacg      240

gataacgaaa aagataacta cctgaaaggt gtgaccaagc tgttcgaacg tatctacagc      300

acggatctgg gtcgcatgct gctgactagc attgttcgcg gtatcccgtt ctggggtggt      360

agcacgattg acaccgaact gaaggttatc gacactaact gcattaacgt tattcaaccg      420

gatggtagct atcgtagcga agagctgaat ctggtcatca ttggcccgag cgcagacatt      480

atccaattcg agtgcaagag ctttggtcac gaggttctga atctgacccg caatggctat      540

ggtagcaccc agtacattcg tttttcgccg gattttacct tcggctttga agagagcctg      600

gaggttgata ccaatccgtt gctgggtgcg ggcaaattcg ctaccgatcc ggctgtcacg      660

ctggcccatg aactgatcca cgcaggccac cgcctgtacg gcattgccat caacccaaac      720

cgtgtgttca aggttaatac gaatgcatac tacgagatga gcggcctgga agtcagcttc      780

gaagaactgc gcaccttcgg tggccatgac gctaaattca ttgacagctt gcaagagaat      840

gagttccgtc tgtactacta taacaaattc aaagacattg caagcacgtt gaacaaggcc      900

aaaagcatcg ttggtactac cgcgtcgttg cagtatatga agaatgtgtt taaagagaag      960

tacctgctgt ccgaggatac ctccggcaag tttagcgttg ataagctgaa gtttgacaaa     1020

ctgtacaaga tgctgaccga gatttacacc gaggacaact ttgtgaaatt cttcaaagtg     1080

ttgaatcgta aaacctatct gaattttgac aaagcggttt tcaagattaa catcgtgccg     1140

aaggtgaact acaccatcta tgacggtttt aacctgcgta acaccaacct ggcggcgaac     1200

tttaacggtc agaatacgga aatcaacaac atgaatttca cgaagttgaa gaacttcacg     1260

ggtctgttcg agttctataa gctgctgtgc gtgcgcggta tcatcaccag caaaaccaaa     1320

agcctggaca aaggctacaa caaggcgctg aatgacctgt gcattaaggt aaacaattgg     1380

gatctgttct tttcgccatc cgaagataat tttaccaacg acctgaacaa gggtgaagaa     1440

atcaccagcg atacgaatat tgaagcagcg gaagagaata tcagcctgga tctgatccag     1500

cagtactatc tgacctttaa cttcgacaat gaaccggaga acattagcat tgagaatctg     1560

agcagcgaca ttatcggtca gctggaactg atgccgaata tcgaacgttt cccgaacggc     1620

aaaaagtacg agctggacaa gtacactatg ttccattacc tgcgtgcaca ggagtttgaa     1680

cacggtaaaa gccgtatcgc gctgaccaac agcgttaacg aggccctgct gaacccgagc     1740

cgtgtctata ccttcttcag cagcgactat gttaagaaag tgaacaaagc cactgaggcc     1800

gcgatgttcc tgggctgggt ggaacagctg gtatatgact tcacggacga gacgagcgaa     1860

gtgagcacta ccgacaaaat tgctgatatt accatcatta tcccgtatat tggtccggca     1920

ctgaacattg gcaacatgct gtacaaagac gattttgtgg gtgccctgat cttctccggt     1980

gccgtgattc tgctggagtt cattccggag attgcgatcc cggtgttggg taccttcgcg     2040

ctggtgtcct acatcgcgaa taaggttctg acggttcaga ccatcgataa cgcgctgtcg     2100

aaacgtaatg aaaaatggga cgaggtttac aaatacattg ttacgaattg gctggcgaaa     2160

gtcaataccc agatcgacct gatccgtaag aaaatgaaag aggcgctgga gaatcaggcg     2220

gaggccacca aagcaattat caactaccaa tacaaccagt acacggaaga agagaagaat     2280

aacattaact tcaatatcga tgatttgagc agcaagctga atgaatctat caacaaagcg     2340

atgatcaata tcaacaagtt tttgaatcag tgtagcgttt cgtacctgat gaatagcatg     2400

attccgtatg gcgtcaaacg tctggaggac ttcgacgcca gcctgaaaga tgcgttgctg     2460

aaatacattt acgacaatcg tggtacgctg attggccaag ttgaccgctt gaaagacaaa     2520

gttaacaata ccctgagcac cgacatccca tttcaactga gcaagtatgt tgataatcaa     2580

cgtctgttga gcactttcac cgagtatatc aaaaacatca tcaatactag cattctgaac     2640

ctgcgttacg agagcaatca tctgattgat ctgagccgtt atgcaagcaa gatcaacatc     2700

ggtagcaagg tcaattttga cccgatcgat aagaaccaga tccagctgtt taatctggaa     2760

tcgagcaaaa ttgaggttat cctgaaaaac gccattgtct acaactccat gtacgagaat     2820

ttctccacca gcttctggat tcgcatcccg aaatacttca acagcattag cctgaacaac     2880

gagtatacta tcatcaactg tatggagaac aacagcggtt ggaaggtgtc tctgaactat     2940

ggtgagatca tttggacctt gcaggacacc caagagatca agcagcgcgt cgtgttcaag     3000

tactctcaaa tgatcaacat ttccgattac attaatcgtt ggatcttcgt gaccattacg     3060

aataaccgtc tgaataacag caagatttac atcaatggtc gcttgatcga tcagaaaccg     3120

attagcaacc tgggtaatat ccacgcaagc aacaacatta tgttcaaatt ggacggttgc     3180

cgcgataccc atcgttatat ctggatcaag tatttcaacc tgtttgataa agaactgaat     3240

gagaaggaga tcaaagattt gtatgacaac caatctaaca gcggcatttt gaaggacttc     3300

tggggcgatt atctgcaata cgataagccg tactatatgc tgaacctgta tgatccgaac     3360

aaatatgtgg atgtcaataa tgtgggtatt cgtggttaca tgtatttgaa gggtccgcgt     3420

ggcagcgtta tgacgaccaa catttacctg aactctagcc tgtaccgtgg tacgaaattc     3480

atcattaaga aatatgccag cggcaacaaa gataacattg tgcgtaataa cgatcgtgtc     3540

tacatcaacg tggtcgtgaa gaataaagag taccgtctgg cgaccaacgc ttcgcaggcg     3600

ggtgttgaga aaattctgag cgcgttggag atccctgatg tcggtaatct gagccaagtc     3660

gtggttatga agagcaagaa cgaccagggt atcactaaca agtgcaagat gaacctgcaa     3720

gacaacaatg gtaacgacat cggctttatt ggtttccacc agttcaacaa tattgctaaa     3780

ctggtagcga gcaattggta caatcgtcag attgagcgca gcagccgtac tttgggctgt     3840

agctgggagt ttatcccggt cgatgatggt tggggcgaac gtccgctg                  3888


<210>  2
<211>  1296
<212>  PRT
<213>  Clostridium botulinum

<400>  2

Met Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly 
1               5                   10                  15      


Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro 
            20                  25                  30          


Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg 
        35                  40                  45              


Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu 
    50                  55                  60                  


Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr 
65                  70                  75                  80  


Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu 
                85                  90                  95      


Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val 
            100                 105                 110         


Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys 
        115                 120                 125             


Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr 
    130                 135                 140                 


Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile 
145                 150                 155                 160 


Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr 
                165                 170                 175     


Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe 
            180                 185                 190         


Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu 
        195                 200                 205             


Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Glu 
    210                 215                 220                 


Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn 
225                 230                 235                 240 


Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu 
                245                 250                 255     


Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys 
            260                 265                 270         


Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn 
        275                 280                 285             


Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val 
    290                 295                 300                 


Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys 
305                 310                 315                 320 


Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu 
                325                 330                 335     


Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp 
            340                 345                 350         


Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu Asn 
        355                 360                 365             


Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr 
    370                 375                 380                 


Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn 
385                 390                 395                 400 


Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu 
                405                 410                 415     


Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Arg 
            420                 425                 430         


Gly Ile Ile Thr Ser Lys Thr Lys Ser Leu Asp Lys Gly Tyr Asn Lys 
        435                 440                 445             


Ala Leu Asn Asp Leu Cys Ile Lys Val Asn Asn Trp Asp Leu Phe Phe 
    450                 455                 460                 


Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys Gly Glu Glu 
465                 470                 475                 480 


Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu Asn Ile Ser Leu 
                485                 490                 495     


Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe Asp Asn Glu Pro 
            500                 505                 510         


Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile Ile Gly Gln Leu 
        515                 520                 525             


Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly Lys Lys Tyr Glu 
    530                 535                 540                 


Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala Gln Glu Phe Glu 
545                 550                 555                 560 


His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val Asn Glu Ala Leu 
                565                 570                 575     


Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp Tyr Val Lys 
            580                 585                 590         


Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu Gly Trp Val Glu 
        595                 600                 605             


Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu Val Ser Thr Thr 
    610                 615                 620                 


Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr Ile Gly Pro Ala 
625                 630                 635                 640 


Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe Val Gly Ala Leu 
                645                 650                 655     


Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile Pro Glu Ile Ala 
            660                 665                 670         


Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile Ala Asn Lys 
        675                 680                 685             


Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser Lys Arg Asn Glu 
    690                 695                 700                 


Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp Leu Ala Lys 
705                 710                 715                 720 


Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met Lys Glu Ala Leu 
                725                 730                 735     


Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn Tyr Gln Tyr Asn 
            740                 745                 750         


Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe Asn Ile Asp Asp 
        755                 760                 765             


Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala Met Ile Asn Ile 
    770                 775                 780                 


Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu Met Asn Ser Met 
785                 790                 795                 800 


Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp Ala Ser Leu Lys 
                805                 810                 815     


Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly Thr Leu Ile Gly 
            820                 825                 830         


Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr Leu Ser Thr Asp 
        835                 840                 845             


Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln Arg Leu Leu Ser 
    850                 855                 860                 


Thr Phe Thr Glu Tyr Ile Lys Asn Ile Ile Asn Thr Ser Ile Leu Asn 
865                 870                 875                 880 


Leu Arg Tyr Glu Ser Asn His Leu Ile Asp Leu Ser Arg Tyr Ala Ser 
                885                 890                 895     


Lys Ile Asn Ile Gly Ser Lys Val Asn Phe Asp Pro Ile Asp Lys Asn 
            900                 905                 910         


Gln Ile Gln Leu Phe Asn Leu Glu Ser Ser Lys Ile Glu Val Ile Leu 
        915                 920                 925             


Lys Asn Ala Ile Val Tyr Asn Ser Met Tyr Glu Asn Phe Ser Thr Ser 
    930                 935                 940                 


Phe Trp Ile Arg Ile Pro Lys Tyr Phe Asn Ser Ile Ser Leu Asn Asn 
945                 950                 955                 960 


Glu Tyr Thr Ile Ile Asn Cys Met Glu Asn Asn Ser Gly Trp Lys Val 
                965                 970                 975     


Ser Leu Asn Tyr Gly Glu Ile Ile Trp Thr Leu Gln Asp Thr Gln Glu 
            980                 985                 990         


Ile Lys Gln Arg Val Val Phe Lys  Tyr Ser Gln Met Ile  Asn Ile Ser 
        995                 1000                 1005             


Asp Tyr  Ile Asn Arg Trp Ile  Phe Val Thr Ile Thr  Asn Asn Arg 
    1010                 1015                 1020             


Leu Asn  Asn Ser Lys Ile Tyr  Ile Asn Gly Arg Leu  Ile Asp Gln 
    1025                 1030                 1035             


Lys Pro  Ile Ser Asn Leu Gly  Asn Ile His Ala Ser  Asn Asn Ile 
    1040                 1045                 1050             


Met Phe  Lys Leu Asp Gly Cys  Arg Asp Thr His Arg  Tyr Ile Trp 
    1055                 1060                 1065             


Ile Lys  Tyr Phe Asn Leu Phe  Asp Lys Glu Leu Asn  Glu Lys Glu 
    1070                 1075                 1080             


Ile Lys  Asp Leu Tyr Asp Asn  Gln Ser Asn Ser Gly  Ile Leu Lys 
    1085                 1090                 1095             


Asp Phe  Trp Gly Asp Tyr Leu  Gln Tyr Asp Lys Pro  Tyr Tyr Met 
    1100                 1105                 1110             


Leu Asn  Leu Tyr Asp Pro Asn  Lys Tyr Val Asp Val  Asn Asn Val 
    1115                 1120                 1125             


Gly Ile  Arg Gly Tyr Met Tyr  Leu Lys Gly Pro Arg  Gly Ser Val 
    1130                 1135                 1140             


Met Thr  Thr Asn Ile Tyr Leu  Asn Ser Ser Leu Tyr  Arg Gly Thr 
    1145                 1150                 1155             


Lys Phe  Ile Ile Lys Lys Tyr  Ala Ser Gly Asn Lys  Asp Asn Ile 
    1160                 1165                 1170             


Val Arg  Asn Asn Asp Arg Val  Tyr Ile Asn Val Val  Val Lys Asn 
    1175                 1180                 1185             


Lys Glu  Tyr Arg Leu Ala Thr  Asn Ala Ser Gln Ala  Gly Val Glu 
    1190                 1195                 1200             


Lys Ile  Leu Ser Ala Leu Glu  Ile Pro Asp Val Gly  Asn Leu Ser 
    1205                 1210                 1215             


Gln Val  Val Val Met Lys Ser  Lys Asn Asp Gln Gly  Ile Thr Asn 
    1220                 1225                 1230             


Lys Cys  Lys Met Asn Leu Gln  Asp Asn Asn Gly Asn  Asp Ile Gly 
    1235                 1240                 1245             


Phe Ile  Gly Phe His Gln Phe  Asn Asn Ile Ala Lys  Leu Val Ala 
    1250                 1255                 1260             


Ser Asn  Trp Tyr Asn Arg Gln  Ile Glu Arg Ser Ser  Arg Thr Leu 
    1265                 1270                 1275             


Gly Cys  Ser Trp Glu Phe Ile  Pro Val Asp Asp Gly  Trp Gly Glu 
    1280                 1285                 1290             


Arg Pro  Leu 
    1295     


<210>  3
<211>  3888
<212>  DNA
<213>  artificial

<220>
<223>  Modified BoNT/A "Cat-A"

<400>  3
atgccattcg tcaacaagca attcaactac aaagacccag tcaacggcgt cgacatcgca       60

tacatcaaga ttccgaacgc cggtcaaatg cagccggtta aggcttttaa gatccacaac      120

aagatttggg ttatcccgga gcgtgacacc ttcacgaacc cggaagaagg cgatctgaac      180

ccgccaccgg aagcgaagca agtccctgtc agctactacg attcgacgta cctgagcacg      240

gataacgaaa aagataacta cctgaaaggt gtgaccaagc tgttcgaacg tatctacagc      300

acggatctgg gtcgcatgct gctgactagc attgttcgcg gtatcccgtt ctggggtggt      360

agcacgattg acaccgaact gaaggttatc gacactaact gcattaacgt tattcaaccg      420

gatggtagct atcgtagcga agagctgaat ctggtcatca ttggcccgag cgcagacatt      480

atccaattcg agtgcaagag ctttggtcac gaggttctga atctgacccg caatggctat      540

ggtagcaccc agtacattcg tttttcgccg gattttacct tcggctttga agagagcctg      600

gaggttgata ccaatccgtt gctgggtgcg ggcaaattcg ctaccgatcc ggctgtcacg      660

ctggcccatg aactgatcca cgcaggccac cgcctgtacg gcattgccat caacccaaac      720

cgtgtgttca aggttaatac gaatgcatac tacgagatga gcggcctgga agtcagcttc      780

gaagaactgc gcaccttcgg tggccatgac gctaaattca ttgacagctt gcaagagaat      840

gagttccgtc tgtactacta taacaaattc aaagacattg caagcacgtt gaacaaggcc      900

aaaagcatcg ttggtactac cgcgtcgttg cagtatatga agaatgtgtt taaagagaag      960

tacctgctgt ccgaggatac ctccggcaag tttagcgttg ataagctgaa gtttgacaaa     1020

ctgtacaaga tgctgaccga gatttacacc gaggacaact ttgtgaaatt cttcaaagtg     1080

ttgaatcgta aaacctatct gaattttgac aaagcggttt tcaagattaa catcgtgccg     1140

aaggtgaact acaccatcta tgacggtttt aacctgcgta acaccaacct ggcggcgaac     1200

tttaacggtc agaatacgga aatcaacaac atgaatttca cgaagttgaa gaacttcacg     1260

ggtctgttcg agttctataa gctgctgtgc gtgcgcggta tcatcaccag caaaaccaaa     1320

agcctggaca aaggctacaa caaggcgctg aatgacctgt gcattaaggt aaacaattgg     1380

gatctgttct tttcgccatc cgaagataat tttaccaacg acctgaacaa gggtgaagaa     1440

atcaccagcg atacgaatat tgaagcagcg gaagagaata tcagcctgga tctgatccag     1500

cagtactatc tgacctttaa cttcgacaat gaaccggaga acattagcat tgagaatctg     1560

agcagcgaca ttatcggtca gctggaactg atgccgaata tcgaacgttt cccgaacggc     1620

aaaaagtacg agctggacaa gtacactatg ttccattacc tgcgtgcaca ggagtttgaa     1680

cacggtaaaa gccgtatcgc gctgaccaac agcgttaacg aggccctgct gaacccgagc     1740

cgtgtctata ccttcttcag cagcgactat gttaagaaag tgaacaaagc cactgaggcc     1800

gcgatgttcc tgggctgggt ggaacagctg gtatatgact tcacggacga gacgagcgaa     1860

gtgagcacta ccgacaaaat tgctgatatt accatcatta tcccgtatat tggtccggca     1920

ctgaacattg gcaacatgct gtacaaagac gattttgtgg gtgccctgat cttctccggt     1980

gccgtgattc tgctggagtt cattccggag attgcgatcc cggtgttggg taccttcgcg     2040

ctggtgtcct acatcgcgaa taaggttctg acggttcaga ccatcgataa cgcgctgtcg     2100

aaacgtaatg aaaaatggga cgaggtttac aaatacattg ttacgaattg gctggcgaaa     2160

gtcaataccc agatcgacct gatccgtaag aaaatgaaag aggcgctgga gaatcaggcg     2220

gaggccacca aagcaattat caactaccaa tacaaccagt acacggaaga agagaagaat     2280

aacattaact tcaatatcga tgatttgagc agcaagctga atgaatctat caacaaagcg     2340

atgatcaata tcaacaagtt tttgaatcag tgtagcgttt cgtacctgat gaatagcatg     2400

attccgtatg gcgtcaaacg tctggaggac ttcgacgcca gcctgaaaga tgcgttgctg     2460

aaatacattt acgacaatcg tggtacgctg attggccaag ttgaccgctt gaaagacaaa     2520

gttaacaata ccctgagcac cgacatccca tttcaactga gcaagtatgt tgataatcaa     2580

cgtctgttga gcactttcac cgagtatatc aaaaacatca tcaatactag cattctgaac     2640

ctgcgttacg agagcaagca tctgattgat ctgagccgtt atgctagcaa gatcaacatc     2700

ggtagcaagg tcaattttga cccgatcgat aagaaccaga tccagctgtt taatctggaa     2760

tcgagcaaaa ttgaggttat cctgaaaaag gccattgtct acaactccat gtacgagaat     2820

ttctccacca gcttctggat tcgcatcccg aaatacttca acaagattag cctgaacaac     2880

gagtatacta tcatcaactg tatggagaac aacagcggtt ggaaggtgtc tctgaactat     2940

ggtgagatca tttggacctt gcaggacacc aaagagatca agcagcgcgt cgtgttcaag     3000

tactctcaaa tgatcaacat ttccgattac attaatcgtt ggatcttcgt gaccattacg     3060

aataaccgtc tgaataagag caagatttac atcaatggtc gcttgatcga tcagaaaccg     3120

attagcaacc tgggtaatat ccacgcaagc aacaagatta tgttcaaatt ggacggttgc     3180

cgcgataccc atcgttatat ctggatcaag tatttcaacc tgtttgataa agaactgaat     3240

gagaaggaga tcaaagattt gtatgacaac caatctaaca gcggcatttt gaaggacttc     3300

tggggcgatt atctgcaata cgataagccg tactatatgc tgaacctgta tgatccgaac     3360

aaatatgtgg atgtcaataa tgtgggtatt cgtggttaca tgtatttgaa gggtccgcgt     3420

ggcagcgtta tgacgaccaa catttacctg aactctagcc tgtaccgtgg tacgaaattc     3480

atcattaaga aatatgccag cggcaacaaa gataacattg tgcgtaataa cgatcgtgtc     3540

tacatcaacg tggtcgtgaa gaataaagag taccgtctgg cgaccaacgc ttcgcaggcg     3600

ggtgttgaga aaattctgag cgcgttggag atccctgatg tcggtaatct gagccaagtc     3660

gtggttatga agagcaagaa cgacaagggt atcactaaca agtgcaagat gaacctgcaa     3720

gacaacaatg gtaacgacat cggctttatt ggtttccacc agttcaacaa tattgctaaa     3780

ctggtagcga gcaattggta caatcgtcag attgagcgca gcagccgtac tttgggctgt     3840

agctgggagt ttatcccggt cgatgatggt tggggcgaac gtccgctg                  3888


<210>  4
<211>  1296
<212>  PRT
<213>  Artificial

<220>
<223>  Modified BoNT/A "Cat-A"

<400>  4

Met Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly 
1               5                   10                  15      


Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro 
            20                  25                  30          


Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg 
        35                  40                  45              


Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu 
    50                  55                  60                  


Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr 
65                  70                  75                  80  


Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu 
                85                  90                  95      


Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val 
            100                 105                 110         


Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys 
        115                 120                 125             


Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr 
    130                 135                 140                 


Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile 
145                 150                 155                 160 


Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr 
                165                 170                 175     


Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe 
            180                 185                 190         


Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu 
        195                 200                 205             


Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Glu 
    210                 215                 220                 


Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn 
225                 230                 235                 240 


Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu 
                245                 250                 255     


Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys 
            260                 265                 270         


Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn 
        275                 280                 285             


Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val 
    290                 295                 300                 


Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys 
305                 310                 315                 320 


Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu 
                325                 330                 335     


Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp 
            340                 345                 350         


Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu Asn 
        355                 360                 365             


Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr 
    370                 375                 380                 


Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn 
385                 390                 395                 400 


Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu 
                405                 410                 415     


Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Arg 
            420                 425                 430         


Gly Ile Ile Thr Ser Lys Thr Lys Ser Leu Asp Lys Gly Tyr Asn Lys 
        435                 440                 445             


Ala Leu Asn Asp Leu Cys Ile Lys Val Asn Asn Trp Asp Leu Phe Phe 
    450                 455                 460                 


Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys Gly Glu Glu 
465                 470                 475                 480 


Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu Asn Ile Ser Leu 
                485                 490                 495     


Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe Asp Asn Glu Pro 
            500                 505                 510         


Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile Ile Gly Gln Leu 
        515                 520                 525             


Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly Lys Lys Tyr Glu 
    530                 535                 540                 


Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala Gln Glu Phe Glu 
545                 550                 555                 560 


His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val Asn Glu Ala Leu 
                565                 570                 575     


Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp Tyr Val Lys 
            580                 585                 590         


Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu Gly Trp Val Glu 
        595                 600                 605             


Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu Val Ser Thr Thr 
    610                 615                 620                 


Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr Ile Gly Pro Ala 
625                 630                 635                 640 


Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe Val Gly Ala Leu 
                645                 650                 655     


Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile Pro Glu Ile Ala 
            660                 665                 670         


Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile Ala Asn Lys 
        675                 680                 685             


Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser Lys Arg Asn Glu 
    690                 695                 700                 


Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp Leu Ala Lys 
705                 710                 715                 720 


Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met Lys Glu Ala Leu 
                725                 730                 735     


Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn Tyr Gln Tyr Asn 
            740                 745                 750         


Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe Asn Ile Asp Asp 
        755                 760                 765             


Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala Met Ile Asn Ile 
    770                 775                 780                 


Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu Met Asn Ser Met 
785                 790                 795                 800 


Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp Ala Ser Leu Lys 
                805                 810                 815     


Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly Thr Leu Ile Gly 
            820                 825                 830         


Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr Leu Ser Thr Asp 
        835                 840                 845             


Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln Arg Leu Leu Ser 
    850                 855                 860                 


Thr Phe Thr Glu Tyr Ile Lys Asn Ile Ile Asn Thr Ser Ile Leu Asn 
865                 870                 875                 880 


Leu Arg Tyr Glu Ser Lys His Leu Ile Asp Leu Ser Arg Tyr Ala Ser 
                885                 890                 895     


Lys Ile Asn Ile Gly Ser Lys Val Asn Phe Asp Pro Ile Asp Lys Asn 
            900                 905                 910         


Gln Ile Gln Leu Phe Asn Leu Glu Ser Ser Lys Ile Glu Val Ile Leu 
        915                 920                 925             


Lys Lys Ala Ile Val Tyr Asn Ser Met Tyr Glu Asn Phe Ser Thr Ser 
    930                 935                 940                 


Phe Trp Ile Arg Ile Pro Lys Tyr Phe Asn Lys Ile Ser Leu Asn Asn 
945                 950                 955                 960 


Glu Tyr Thr Ile Ile Asn Cys Met Glu Asn Asn Ser Gly Trp Lys Val 
                965                 970                 975     


Ser Leu Asn Tyr Gly Glu Ile Ile Trp Thr Leu Gln Asp Thr Lys Glu 
            980                 985                 990         


Ile Lys Gln Arg Val Val Phe Lys  Tyr Ser Gln Met Ile  Asn Ile Ser 
        995                 1000                 1005             


Asp Tyr  Ile Asn Arg Trp Ile  Phe Val Thr Ile Thr  Asn Asn Arg 
    1010                 1015                 1020             


Leu Asn  Lys Ser Lys Ile Tyr  Ile Asn Gly Arg Leu  Ile Asp Gln 
    1025                 1030                 1035             


Lys Pro  Ile Ser Asn Leu Gly  Asn Ile His Ala Ser  Asn Lys Ile 
    1040                 1045                 1050             


Met Phe  Lys Leu Asp Gly Cys  Arg Asp Thr His Arg  Tyr Ile Trp 
    1055                 1060                 1065             


Ile Lys  Tyr Phe Asn Leu Phe  Asp Lys Glu Leu Asn  Glu Lys Glu 
    1070                 1075                 1080             


Ile Lys  Asp Leu Tyr Asp Asn  Gln Ser Asn Ser Gly  Ile Leu Lys 
    1085                 1090                 1095             


Asp Phe  Trp Gly Asp Tyr Leu  Gln Tyr Asp Lys Pro  Tyr Tyr Met 
    1100                 1105                 1110             


Leu Asn  Leu Tyr Asp Pro Asn  Lys Tyr Val Asp Val  Asn Asn Val 
    1115                 1120                 1125             


Gly Ile  Arg Gly Tyr Met Tyr  Leu Lys Gly Pro Arg  Gly Ser Val 
    1130                 1135                 1140             


Met Thr  Thr Asn Ile Tyr Leu  Asn Ser Ser Leu Tyr  Arg Gly Thr 
    1145                 1150                 1155             


Lys Phe  Ile Ile Lys Lys Tyr  Ala Ser Gly Asn Lys  Asp Asn Ile 
    1160                 1165                 1170             


Val Arg  Asn Asn Asp Arg Val  Tyr Ile Asn Val Val  Val Lys Asn 
    1175                 1180                 1185             


Lys Glu  Tyr Arg Leu Ala Thr  Asn Ala Ser Gln Ala  Gly Val Glu 
    1190                 1195                 1200             


Lys Ile  Leu Ser Ala Leu Glu  Ile Pro Asp Val Gly  Asn Leu Ser 
    1205                 1210                 1215             


Gln Val  Val Val Met Lys Ser  Lys Asn Asp Lys Gly  Ile Thr Asn 
    1220                 1225                 1230             


Lys Cys  Lys Met Asn Leu Gln  Asp Asn Asn Gly Asn  Asp Ile Gly 
    1235                 1240                 1245             


Phe Ile  Gly Phe His Gln Phe  Asn Asn Ile Ala Lys  Leu Val Ala 
    1250                 1255                 1260             


Ser Asn  Trp Tyr Asn Arg Gln  Ile Glu Arg Ser Ser  Arg Thr Leu 
    1265                 1270                 1275             


Gly Cys  Ser Trp Glu Phe Ile  Pro Val Asp Asp Gly  Trp Gly Glu 
    1280                 1285                 1290             


Arg Pro  Leu 
    1295     


<210>  5
<211>  3888
<212>  DNA
<213>  Artificial

<220>
<223>  Modified BoNT/A "Cat-B"

<400>  5
atgccattcg tcaacaagca attcaactac aaagacccag tcaacggcgt cgacatcgca       60

tacatcaaga ttccgaacgc cggtcaaatg cagccggtta aggcttttaa gatccacaac      120

aagatttggg ttatcccgga gcgtgacacc ttcacgaacc cggaagaagg cgatctgaac      180

ccgccaccgg aagcgaagca agtccctgtc agctactacg attcgacgta cctgagcacg      240

gataacgaaa aagataacta cctgaaaggt gtgaccaagc tgttcgaacg tatctacagc      300

acggatctgg gtcgcatgct gctgactagc attgttcgcg gtatcccgtt ctggggtggt      360

agcacgattg acaccgaact gaaggttatc gacactaact gcattaacgt tattcaaccg      420

gatggtagct atcgtagcga agagctgaat ctggtcatca ttggcccgag cgcagacatt      480

atccaattcg agtgcaagag ctttggtcac gaggttctga atctgacccg caatggctat      540

ggtagcaccc agtacattcg tttttcgccg gattttacct tcggctttga agagagcctg      600

gaggttgata ccaatccgtt gctgggtgcg ggcaaattcg ctaccgatcc ggctgtcacg      660

ctggcccatg aactgatcca cgcaggccac cgcctgtacg gcattgccat caacccaaac      720

cgtgtgttca aggttaatac gaatgcatac tacgagatga gcggcctgga agtcagcttc      780

gaagaactgc gcaccttcgg tggccatgac gctaaattca ttgacagctt gcaagagaat      840

gagttccgtc tgtactacta taacaaattc aaagacattg caagcacgtt gaacaaggcc      900

aaaagcatcg ttggtactac cgcgtcgttg cagtatatga agaatgtgtt taaagagaag      960

tacctgctgt ccgaggatac ctccggcaag tttagcgttg ataagctgaa gtttgacaaa     1020

ctgtacaaga tgctgaccga gatttacacc gaggacaact ttgtgaaatt cttcaaagtg     1080

ttgaatcgta aaacctatct gaattttgac aaagcggttt tcaagattaa catcgtgccg     1140

aaggtgaact acaccatcta tgacggtttt aacctgcgta acaccaacct ggcggcgaac     1200

tttaacggtc agaatacgga aatcaacaac atgaatttca cgaagttgaa gaacttcacg     1260

ggtctgttcg agttctataa gctgctgtgc gtgcgcggta tcatcaccag caaaaccaaa     1320

agcctggaca aaggctacaa caaggcgctg aatgacctgt gcattaaggt aaacaattgg     1380

gatctgttct tttcgccatc cgaagataat tttaccaacg acctgaacaa gggtgaagaa     1440

atcaccagcg atacgaatat tgaagcagcg gaagagaata tcagcctgga tctgatccag     1500

cagtactatc tgacctttaa cttcgacaat gaaccggaga acattagcat tgagaatctg     1560

agcagcgaca ttatcggtca gctggaactg atgccgaata tcgaacgttt cccgaacggc     1620

aaaaagtacg agctggacaa gtacactatg ttccattacc tgcgtgcaca ggagtttgaa     1680

cacggtaaaa gccgtatcgc gctgaccaac agcgttaacg aggccctgct gaacccgagc     1740

cgtgtctata ccttcttcag cagcgactat gttaagaaag tgaacaaagc cactgaggcc     1800

gcgatgttcc tgggctgggt ggaacagctg gtatatgact tcacggacga gacgagcgaa     1860

gtgagcacta ccgacaaaat tgctgatatt accatcatta tcccgtatat tggtccggca     1920

ctgaacattg gcaacatgct gtacaaagac gattttgtgg gtgccctgat cttctccggt     1980

gccgtgattc tgctggagtt cattccggag attgcgatcc cggtgttggg taccttcgcg     2040

ctggtgtcct acatcgcgaa taaggttctg acggttcaga ccatcgataa cgcgctgtcg     2100

aaacgtaatg aaaaatggga cgaggtttac aaatacattg ttacgaattg gctggcgaaa     2160

gtcaataccc agatcgacct gatccgtaag aaaatgaaag aggcgctgga gaatcaggcg     2220

gaggccacca aagcaattat caactaccaa tacaaccagt acacggaaga agagaagaat     2280

aacattaact tcaatatcga tgatttgagc agcaagctga atgaatctat caacaaagcg     2340

atgatcaata tcaacaagtt tttgaatcag tgtagcgttt cgtacctgat gaatagcatg     2400

attccgtatg gcgtcaaacg tctggaggac ttcgacgcca gcctgaaaga tgcgttgctg     2460

aaatacattt acgacaatcg tggtacgctg attggccaag ttgaccgctt gaaagacaaa     2520

gttaacaata ccctgagcac cgacatccca tttcaactga gcaagtatgt tgataatcaa     2580

cgtctgttga gcactttcac cgagtatatc aaaaacatca tcaatactag cattctgaac     2640

ctgcgttacg agagcaatca tctgattgat ctgagccgtt atgctagcaa gatcaacatc     2700

ggtagcaagg tcaattttga cccgatcgat aagaaccaga tccagctgtt taatctggaa     2760

tcgagcaaaa ttgaggttat cctgaaaaag gccattgtct acaactccat gtacgagaat     2820

ttctccacca gcttctggat tcgcatcccg aaatacttca agaagattag cctgaacaac     2880

gagtatacta tcatcaactg tatggagaac aacagcggtt ggaaggtgtc tctgaactat     2940

ggtgagatca tttggacctt gcaggacacc aaagagatca agcagcgcgt cgtgttcaag     3000

tactctcaaa tgatcaacat ttccgattac attaatcgtt ggatcttcgt gaccattacg     3060

aataaccgtc tgaataagag caagatttac atcaatggtc gcttgatcga tcagaaaccg     3120

attagcaacc tgggtaatat ccacgcaagc aacaagatta tgttcaaatt ggacggttgc     3180

cgcgataccc atcgttatat ctggatcaag tatttcaacc tgtttgataa agaactgaat     3240

gagaaggaga tcaaagattt gtatgacaac caatctaaca gcggcatttt gaaggacttc     3300

tggggcgatt atctgcaata cgataagccg tactatatgc tgaacctgta tgatccgaac     3360

aaatatgtgg atgtcaataa tgtgggtatt cgtggttaca tgtatttgaa gggtccgcgt     3420

ggcagcgtta tgacgaccaa catttacctg aactctagcc tgtaccgtgg tacgaaattc     3480

atcattaaga aatatgccag cggcaacaaa gataacattg tgcgtaataa cgatcgtgtc     3540

tacatcaacg tggtcgtgaa gaataaagag taccgtctgg cgaccaacgc ttcgcaggcg     3600

ggtgttgaga aaattctgag cgcgttggag atccctgatg tcggtaatct gagccaagtc     3660

gtggttatga agagcaagaa cgacaagggt atcactaaca agtgcaagat gaacctgcaa     3720

gacaacaatg gtaacgacat cggctttatt ggtttccacc agttcaacaa tattgctaaa     3780

ctggtagcga gcaattggta caatcgtcag attgagcgca gcagccgtac tttgggctgt     3840

agctgggagt ttatcccggt cgatgatggt tggggcgaac gtccgctg                  3888


<210>  6
<211>  1296
<212>  PRT
<213>  artificial

<220>
<223>  Modified BoNT/A "Cat-B"

<400>  6

Met Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly 
1               5                   10                  15      


Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro 
            20                  25                  30          


Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg 
        35                  40                  45              


Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu 
    50                  55                  60                  


Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr 
65                  70                  75                  80  


Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu 
                85                  90                  95      


Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val 
            100                 105                 110         


Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys 
        115                 120                 125             


Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr 
    130                 135                 140                 


Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile 
145                 150                 155                 160 


Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr 
                165                 170                 175     


Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe 
            180                 185                 190         


Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu 
        195                 200                 205             


Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Glu 
    210                 215                 220                 


Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn 
225                 230                 235                 240 


Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu 
                245                 250                 255     


Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys 
            260                 265                 270         


Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn 
        275                 280                 285             


Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val 
    290                 295                 300                 


Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys 
305                 310                 315                 320 


Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu 
                325                 330                 335     


Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp 
            340                 345                 350         


Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu Asn 
        355                 360                 365             


Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr 
    370                 375                 380                 


Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn 
385                 390                 395                 400 


Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu 
                405                 410                 415     


Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Arg 
            420                 425                 430         


Gly Ile Ile Thr Ser Lys Thr Lys Ser Leu Asp Lys Gly Tyr Asn Lys 
        435                 440                 445             


Ala Leu Asn Asp Leu Cys Ile Lys Val Asn Asn Trp Asp Leu Phe Phe 
    450                 455                 460                 


Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys Gly Glu Glu 
465                 470                 475                 480 


Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu Asn Ile Ser Leu 
                485                 490                 495     


Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe Asp Asn Glu Pro 
            500                 505                 510         


Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile Ile Gly Gln Leu 
        515                 520                 525             


Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly Lys Lys Tyr Glu 
    530                 535                 540                 


Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala Gln Glu Phe Glu 
545                 550                 555                 560 


His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val Asn Glu Ala Leu 
                565                 570                 575     


Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp Tyr Val Lys 
            580                 585                 590         


Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu Gly Trp Val Glu 
        595                 600                 605             


Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu Val Ser Thr Thr 
    610                 615                 620                 


Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr Ile Gly Pro Ala 
625                 630                 635                 640 


Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe Val Gly Ala Leu 
                645                 650                 655     


Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile Pro Glu Ile Ala 
            660                 665                 670         


Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile Ala Asn Lys 
        675                 680                 685             


Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser Lys Arg Asn Glu 
    690                 695                 700                 


Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp Leu Ala Lys 
705                 710                 715                 720 


Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met Lys Glu Ala Leu 
                725                 730                 735     


Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn Tyr Gln Tyr Asn 
            740                 745                 750         


Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe Asn Ile Asp Asp 
        755                 760                 765             


Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala Met Ile Asn Ile 
    770                 775                 780                 


Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu Met Asn Ser Met 
785                 790                 795                 800 


Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp Ala Ser Leu Lys 
                805                 810                 815     


Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly Thr Leu Ile Gly 
            820                 825                 830         


Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr Leu Ser Thr Asp 
        835                 840                 845             


Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln Arg Leu Leu Ser 
    850                 855                 860                 


Thr Phe Thr Glu Tyr Ile Lys Asn Ile Ile Asn Thr Ser Ile Leu Asn 
865                 870                 875                 880 


Leu Arg Tyr Glu Ser Asn His Leu Ile Asp Leu Ser Arg Tyr Ala Ser 
                885                 890                 895     


Lys Ile Asn Ile Gly Ser Lys Val Asn Phe Asp Pro Ile Asp Lys Asn 
            900                 905                 910         


Gln Ile Gln Leu Phe Asn Leu Glu Ser Ser Lys Ile Glu Val Ile Leu 
        915                 920                 925             


Lys Lys Ala Ile Val Tyr Asn Ser Met Tyr Glu Asn Phe Ser Thr Ser 
    930                 935                 940                 


Phe Trp Ile Arg Ile Pro Lys Tyr Phe Lys Lys Ile Ser Leu Asn Asn 
945                 950                 955                 960 


Glu Tyr Thr Ile Ile Asn Cys Met Glu Asn Asn Ser Gly Trp Lys Val 
                965                 970                 975     


Ser Leu Asn Tyr Gly Glu Ile Ile Trp Thr Leu Gln Asp Thr Lys Glu 
            980                 985                 990         


Ile Lys Gln Arg Val Val Phe Lys  Tyr Ser Gln Met Ile  Asn Ile Ser 
        995                 1000                 1005             


Asp Tyr  Ile Asn Arg Trp Ile  Phe Val Thr Ile Thr  Asn Asn Arg 
    1010                 1015                 1020             


Leu Asn  Lys Ser Lys Ile Tyr  Ile Asn Gly Arg Leu  Ile Asp Gln 
    1025                 1030                 1035             


Lys Pro  Ile Ser Asn Leu Gly  Asn Ile His Ala Ser  Asn Lys Ile 
    1040                 1045                 1050             


Met Phe  Lys Leu Asp Gly Cys  Arg Asp Thr His Arg  Tyr Ile Trp 
    1055                 1060                 1065             


Ile Lys  Tyr Phe Asn Leu Phe  Asp Lys Glu Leu Asn  Glu Lys Glu 
    1070                 1075                 1080             


Ile Lys  Asp Leu Tyr Asp Asn  Gln Ser Asn Ser Gly  Ile Leu Lys 
    1085                 1090                 1095             


Asp Phe  Trp Gly Asp Tyr Leu  Gln Tyr Asp Lys Pro  Tyr Tyr Met 
    1100                 1105                 1110             


Leu Asn  Leu Tyr Asp Pro Asn  Lys Tyr Val Asp Val  Asn Asn Val 
    1115                 1120                 1125             


Gly Ile  Arg Gly Tyr Met Tyr  Leu Lys Gly Pro Arg  Gly Ser Val 
    1130                 1135                 1140             


Met Thr  Thr Asn Ile Tyr Leu  Asn Ser Ser Leu Tyr  Arg Gly Thr 
    1145                 1150                 1155             


Lys Phe  Ile Ile Lys Lys Tyr  Ala Ser Gly Asn Lys  Asp Asn Ile 
    1160                 1165                 1170             


Val Arg  Asn Asn Asp Arg Val  Tyr Ile Asn Val Val  Val Lys Asn 
    1175                 1180                 1185             


Lys Glu  Tyr Arg Leu Ala Thr  Asn Ala Ser Gln Ala  Gly Val Glu 
    1190                 1195                 1200             


Lys Ile  Leu Ser Ala Leu Glu  Ile Pro Asp Val Gly  Asn Leu Ser 
    1205                 1210                 1215             


Gln Val  Val Val Met Lys Ser  Lys Asn Asp Lys Gly  Ile Thr Asn 
    1220                 1225                 1230             


Lys Cys  Lys Met Asn Leu Gln  Asp Asn Asn Gly Asn  Asp Ile Gly 
    1235                 1240                 1245             


Phe Ile  Gly Phe His Gln Phe  Asn Asn Ile Ala Lys  Leu Val Ala 
    1250                 1255                 1260             


Ser Asn  Trp Tyr Asn Arg Gln  Ile Glu Arg Ser Ser  Arg Thr Leu 
    1265                 1270                 1275             


Gly Cys  Ser Trp Glu Phe Ile  Pro Val Asp Asp Gly  Trp Gly Glu 
    1280                 1285                 1290             


Arg Pro  Leu 
    1295     


<210>  7
<211>  3888
<212>  DNA
<213>  artificial

<220>
<223>  Modified BoNT/A "Cat-C"

<400>  7
atgccattcg tcaacaagca attcaactac aaagacccag tcaacggcgt cgacatcgca       60

tacatcaaga ttccgaacgc cggtcaaatg cagccggtta aggcttttaa gatccacaac      120

aagatttggg ttatcccgga gcgtgacacc ttcacgaacc cggaagaagg cgatctgaac      180

ccgccaccgg aagcgaagca agtccctgtc agctactacg attcgacgta cctgagcacg      240

gataacgaaa aagataacta cctgaaaggt gtgaccaagc tgttcgaacg tatctacagc      300

acggatctgg gtcgcatgct gctgactagc attgttcgcg gtatcccgtt ctggggtggt      360

agcacgattg acaccgaact gaaggttatc gacactaact gcattaacgt tattcaaccg      420

gatggtagct atcgtagcga agagctgaat ctggtcatca ttggcccgag cgcagacatt      480

atccaattcg agtgcaagag ctttggtcac gaggttctga atctgacccg caatggctat      540

ggtagcaccc agtacattcg tttttcgccg gattttacct tcggctttga agagagcctg      600

gaggttgata ccaatccgtt gctgggtgcg ggcaaattcg ctaccgatcc ggctgtcacg      660

ctggcccatg aactgatcca cgcaggccac cgcctgtacg gcattgccat caacccaaac      720

cgtgtgttca aggttaatac gaatgcatac tacgagatga gcggcctgga agtcagcttc      780

gaagaactgc gcaccttcgg tggccatgac gctaaattca ttgacagctt gcaagagaat      840

gagttccgtc tgtactacta taacaaattc aaagacattg caagcacgtt gaacaaggcc      900

aaaagcatcg ttggtactac cgcgtcgttg cagtatatga agaatgtgtt taaagagaag      960

tacctgctgt ccgaggatac ctccggcaag tttagcgttg ataagctgaa gtttgacaaa     1020

ctgtacaaga tgctgaccga gatttacacc gaggacaact ttgtgaaatt cttcaaagtg     1080

ttgaatcgta aaacctatct gaattttgac aaagcggttt tcaagattaa catcgtgccg     1140

aaggtgaact acaccatcta tgacggtttt aacctgcgta acaccaacct ggcggcgaac     1200

tttaacggtc agaatacgga aatcaacaac atgaatttca cgaagttgaa gaacttcacg     1260

ggtctgttcg agttctataa gctgctgtgc gtgcgcggta tcatcaccag caaaaccaaa     1320

agcctggaca aaggctacaa caaggcgctg aatgacctgt gcattaaggt aaacaattgg     1380

gatctgttct tttcgccatc cgaagataat tttaccaacg acctgaacaa gggtgaagaa     1440

atcaccagcg atacgaatat tgaagcagcg gaagagaata tcagcctgga tctgatccag     1500

cagtactatc tgacctttaa cttcgacaat gaaccggaga acattagcat tgagaatctg     1560

agcagcgaca ttatcggtca gctggaactg atgccgaata tcgaacgttt cccgaacggc     1620

aaaaagtacg agctggacaa gtacactatg ttccattacc tgcgtgcaca ggagtttgaa     1680

cacggtaaaa gccgtatcgc gctgaccaac agcgttaacg aggccctgct gaacccgagc     1740

cgtgtctata ccttcttcag cagcgactat gttaagaaag tgaacaaagc cactgaggcc     1800

gcgatgttcc tgggctgggt ggaacagctg gtatatgact tcacggacga gacgagcgaa     1860

gtgagcacta ccgacaaaat tgctgatatt accatcatta tcccgtatat tggtccggca     1920

ctgaacattg gcaacatgct gtacaaagac gattttgtgg gtgccctgat cttctccggt     1980

gccgtgattc tgctggagtt cattccggag attgcgatcc cggtgttggg taccttcgcg     2040

ctggtgtcct acatcgcgaa taaggttctg acggttcaga ccatcgataa cgcgctgtcg     2100

aaacgtaatg aaaaatggga cgaggtttac aaatacattg ttacgaattg gctggcgaaa     2160

gtcaataccc agatcgacct gatccgtaag aaaatgaaag aggcgctgga gaatcaggcg     2220

gaggccacca aagcaattat caactaccaa tacaaccagt acacggaaga agagaagaat     2280

aacattaact tcaatatcga tgatttgagc agcaagctga atgaatctat caacaaagcg     2340

atgatcaata tcaacaagtt tttgaatcag tgtagcgttt cgtacctgat gaatagcatg     2400

attccgtatg gcgtcaaacg tctggaggac ttcgacgcca gcctgaaaga tgcgttgctg     2460

aaatacattt acgacaatcg tggtacgctg attggccaag ttgaccgctt gaaagacaaa     2520

gttaacaata ccctgagcac cgacatccca tttcaactga gcaagtatgt tgataatcaa     2580

cgtctgttga gcactttcac cgagtatatc aaaaacatca tcaatactag cattctgaac     2640

ctgcgttacg agagcaatca tctgattgat ctgagccgtt atgctagcaa gatcaacatc     2700

ggtagcaagg tcaattttga cccgatcgat aagaaccaga tccagctgtt taatctggaa     2760

tcgagcaaaa ttgaggttat cctgaaaaag gccattgtct acaactccat gtacgagaat     2820

ttctccacca gcttctggat tcgcatcccg aaatacttca acaagattag cctgaacaac     2880

gagtatacta tcatcaactg tatggagaac aacagcggtt ggaaggtgtc tctgaactat     2940

ggtgagatca tttggacctt gcaggacacc aaagagatca agcagcgcgt cgtgttcaag     3000

tactctcaaa tgatcaacat ttccgattac attaatcgtt ggatcttcgt gaccattacg     3060

aataaccgtc tgaagaagag caagatttac atcaatggtc gcttgatcga tcagaaaccg     3120

attagcaacc tgggtaatat ccacgcaagc aacaagatta tgttcaaatt ggacggttgc     3180

cgcgataccc atcgttatat ctggatcaag tatttcaacc tgtttgataa agaactgaat     3240

gagaaggaga tcaaagattt gtatgacaac caatctaaca gcggcatttt gaaggacttc     3300

tggggcgatt atctgcaata cgataagccg tactatatgc tgaacctgta tgatccgaac     3360

aaatatgtgg atgtcaataa tgtgggtatt cgtggttaca tgtatttgaa gggtccgcgt     3420

ggcagcgtta tgacgaccaa catttacctg aactctagcc tgtaccgtgg tacgaaattc     3480

atcattaaga aatatgccag cggcaacaaa gataacattg tgcgtaataa cgatcgtgtc     3540

tacatcaacg tggtcgtgaa gaataaagag taccgtctgg cgaccaacgc ttcgcaggcg     3600

ggtgttgaga aaattctgag cgcgttggag atccctgatg tcggtaatct gagccaagtc     3660

gtggttatga agagcaagaa cgacaagggt atcactaaca agtgcaagat gaacctgcaa     3720

gacaacaatg gtaacgacat cggctttatt ggtttccacc agttcaacaa tattgctaaa     3780

ctggtagcga gcaattggta caatcgtcag attgagcgca gcagccgtac tttgggctgt     3840

agctgggagt ttatcccggt cgatgatggt tggggcgaac gtccgctg                  3888


<210>  8
<211>  1296
<212>  PRT
<213>  artificial

<220>
<223>  Modified BoNT/A "Cat-C"

<400>  8

Met Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly 
1               5                   10                  15      


Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro 
            20                  25                  30          


Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg 
        35                  40                  45              


Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu 
    50                  55                  60                  


Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr 
65                  70                  75                  80  


Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu 
                85                  90                  95      


Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val 
            100                 105                 110         


Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys 
        115                 120                 125             


Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr 
    130                 135                 140                 


Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile 
145                 150                 155                 160 


Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr 
                165                 170                 175     


Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe 
            180                 185                 190         


Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu 
        195                 200                 205             


Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Glu 
    210                 215                 220                 


Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn 
225                 230                 235                 240 


Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu 
                245                 250                 255     


Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys 
            260                 265                 270         


Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn 
        275                 280                 285             


Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val 
    290                 295                 300                 


Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys 
305                 310                 315                 320 


Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu 
                325                 330                 335     


Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp 
            340                 345                 350         


Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu Asn 
        355                 360                 365             


Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr 
    370                 375                 380                 


Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn 
385                 390                 395                 400 


Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu 
                405                 410                 415     


Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Arg 
            420                 425                 430         


Gly Ile Ile Thr Ser Lys Thr Lys Ser Leu Asp Lys Gly Tyr Asn Lys 
        435                 440                 445             


Ala Leu Asn Asp Leu Cys Ile Lys Val Asn Asn Trp Asp Leu Phe Phe 
    450                 455                 460                 


Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys Gly Glu Glu 
465                 470                 475                 480 


Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu Asn Ile Ser Leu 
                485                 490                 495     


Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe Asp Asn Glu Pro 
            500                 505                 510         


Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile Ile Gly Gln Leu 
        515                 520                 525             


Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly Lys Lys Tyr Glu 
    530                 535                 540                 


Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala Gln Glu Phe Glu 
545                 550                 555                 560 


His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val Asn Glu Ala Leu 
                565                 570                 575     


Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp Tyr Val Lys 
            580                 585                 590         


Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu Gly Trp Val Glu 
        595                 600                 605             


Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu Val Ser Thr Thr 
    610                 615                 620                 


Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr Ile Gly Pro Ala 
625                 630                 635                 640 


Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe Val Gly Ala Leu 
                645                 650                 655     


Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile Pro Glu Ile Ala 
            660                 665                 670         


Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile Ala Asn Lys 
        675                 680                 685             


Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser Lys Arg Asn Glu 
    690                 695                 700                 


Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp Leu Ala Lys 
705                 710                 715                 720 


Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met Lys Glu Ala Leu 
                725                 730                 735     


Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn Tyr Gln Tyr Asn 
            740                 745                 750         


Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe Asn Ile Asp Asp 
        755                 760                 765             


Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala Met Ile Asn Ile 
    770                 775                 780                 


Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu Met Asn Ser Met 
785                 790                 795                 800 


Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp Ala Ser Leu Lys 
                805                 810                 815     


Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly Thr Leu Ile Gly 
            820                 825                 830         


Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr Leu Ser Thr Asp 
        835                 840                 845             


Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln Arg Leu Leu Ser 
    850                 855                 860                 


Thr Phe Thr Glu Tyr Ile Lys Asn Ile Ile Asn Thr Ser Ile Leu Asn 
865                 870                 875                 880 


Leu Arg Tyr Glu Ser Asn His Leu Ile Asp Leu Ser Arg Tyr Ala Ser 
                885                 890                 895     


Lys Ile Asn Ile Gly Ser Lys Val Asn Phe Asp Pro Ile Asp Lys Asn 
            900                 905                 910         


Gln Ile Gln Leu Phe Asn Leu Glu Ser Ser Lys Ile Glu Val Ile Leu 
        915                 920                 925             


Lys Lys Ala Ile Val Tyr Asn Ser Met Tyr Glu Asn Phe Ser Thr Ser 
    930                 935                 940                 


Phe Trp Ile Arg Ile Pro Lys Tyr Phe Asn Lys Ile Ser Leu Asn Asn 
945                 950                 955                 960 


Glu Tyr Thr Ile Ile Asn Cys Met Glu Asn Asn Ser Gly Trp Lys Val 
                965                 970                 975     


Ser Leu Asn Tyr Gly Glu Ile Ile Trp Thr Leu Gln Asp Thr Lys Glu 
            980                 985                 990         


Ile Lys Gln Arg Val Val Phe Lys  Tyr Ser Gln Met Ile  Asn Ile Ser 
        995                 1000                 1005             


Asp Tyr  Ile Asn Arg Trp Ile  Phe Val Thr Ile Thr  Asn Asn Arg 
    1010                 1015                 1020             


Leu Lys  Lys Ser Lys Ile Tyr  Ile Asn Gly Arg Leu  Ile Asp Gln 
    1025                 1030                 1035             


Lys Pro  Ile Ser Asn Leu Gly  Asn Ile His Ala Ser  Asn Lys Ile 
    1040                 1045                 1050             


Met Phe  Lys Leu Asp Gly Cys  Arg Asp Thr His Arg  Tyr Ile Trp 
    1055                 1060                 1065             


Ile Lys  Tyr Phe Asn Leu Phe  Asp Lys Glu Leu Asn  Glu Lys Glu 
    1070                 1075                 1080             


Ile Lys  Asp Leu Tyr Asp Asn  Gln Ser Asn Ser Gly  Ile Leu Lys 
    1085                 1090                 1095             


Asp Phe  Trp Gly Asp Tyr Leu  Gln Tyr Asp Lys Pro  Tyr Tyr Met 
    1100                 1105                 1110             


Leu Asn  Leu Tyr Asp Pro Asn  Lys Tyr Val Asp Val  Asn Asn Val 
    1115                 1120                 1125             


Gly Ile  Arg Gly Tyr Met Tyr  Leu Lys Gly Pro Arg  Gly Ser Val 
    1130                 1135                 1140             


Met Thr  Thr Asn Ile Tyr Leu  Asn Ser Ser Leu Tyr  Arg Gly Thr 
    1145                 1150                 1155             


Lys Phe  Ile Ile Lys Lys Tyr  Ala Ser Gly Asn Lys  Asp Asn Ile 
    1160                 1165                 1170             


Val Arg  Asn Asn Asp Arg Val  Tyr Ile Asn Val Val  Val Lys Asn 
    1175                 1180                 1185             


Lys Glu  Tyr Arg Leu Ala Thr  Asn Ala Ser Gln Ala  Gly Val Glu 
    1190                 1195                 1200             


Lys Ile  Leu Ser Ala Leu Glu  Ile Pro Asp Val Gly  Asn Leu Ser 
    1205                 1210                 1215             


Gln Val  Val Val Met Lys Ser  Lys Asn Asp Lys Gly  Ile Thr Asn 
    1220                 1225                 1230             


Lys Cys  Lys Met Asn Leu Gln  Asp Asn Asn Gly Asn  Asp Ile Gly 
    1235                 1240                 1245             


Phe Ile  Gly Phe His Gln Phe  Asn Asn Ile Ala Lys  Leu Val Ala 
    1250                 1255                 1260             


Ser Asn  Trp Tyr Asn Arg Gln  Ile Glu Arg Ser Ser  Arg Thr Leu 
    1265                 1270                 1275             


Gly Cys  Ser Trp Glu Phe Ile  Pro Val Asp Asp Gly  Trp Gly Glu 
    1280                 1285                 1290             


Arg Pro  Leu 
    1295     


<210>  9
<211>  3888
<212>  DNA
<213>  artificial

<220>
<223>  Modified BoNT/A "Cat-D"

<400>  9
atgccattcg tcaacaagca attcaactac aaagacccag tcaacggcgt cgacatcgca       60

tacatcaaga ttccgaacgc cggtcaaatg cagccggtta aggcttttaa gatccacaac      120

aagatttggg ttatcccgga gcgtgacacc ttcacgaacc cggaagaagg cgatctgaac      180

ccgccaccgg aagcgaagca agtccctgtc agctactacg attcgacgta cctgagcacg      240

gataacgaaa aagataacta cctgaaaggt gtgaccaagc tgttcgaacg tatctacagc      300

acggatctgg gtcgcatgct gctgactagc attgttcgcg gtatcccgtt ctggggtggt      360

agcacgattg acaccgaact gaaggttatc gacactaact gcattaacgt tattcaaccg      420

gatggtagct atcgtagcga agagctgaat ctggtcatca ttggcccgag cgcagacatt      480

atccaattcg agtgcaagag ctttggtcac gaggttctga atctgacccg caatggctat      540

ggtagcaccc agtacattcg tttttcgccg gattttacct tcggctttga agagagcctg      600

gaggttgata ccaatccgtt gctgggtgcg ggcaaattcg ctaccgatcc ggctgtcacg      660

ctggcccatg aactgatcca cgcaggccac cgcctgtacg gcattgccat caacccaaac      720

cgtgtgttca aggttaatac gaatgcatac tacgagatga gcggcctgga agtcagcttc      780

gaagaactgc gcaccttcgg tggccatgac gctaaattca ttgacagctt gcaagagaat      840

gagttccgtc tgtactacta taacaaattc aaagacattg caagcacgtt gaacaaggcc      900

aaaagcatcg ttggtactac cgcgtcgttg cagtatatga agaatgtgtt taaagagaag      960

tacctgctgt ccgaggatac ctccggcaag tttagcgttg ataagctgaa gtttgacaaa     1020

ctgtacaaga tgctgaccga gatttacacc gaggacaact ttgtgaaatt cttcaaagtg     1080

ttgaatcgta aaacctatct gaattttgac aaagcggttt tcaagattaa catcgtgccg     1140

aaggtgaact acaccatcta tgacggtttt aacctgcgta acaccaacct ggcggcgaac     1200

tttaacggtc agaatacgga aatcaacaac atgaatttca cgaagttgaa gaacttcacg     1260

ggtctgttcg agttctataa gctgctgtgc gtgcgcggta tcatcaccag caaaaccaaa     1320

agcctggaca aaggctacaa caaggcgctg aatgacctgt gcattaaggt aaacaattgg     1380

gatctgttct tttcgccatc cgaagataat tttaccaacg acctgaacaa gggtgaagaa     1440

atcaccagcg atacgaatat tgaagcagcg gaagagaata tcagcctgga tctgatccag     1500

cagtactatc tgacctttaa cttcgacaat gaaccggaga acattagcat tgagaatctg     1560

agcagcgaca ttatcggtca gctggaactg atgccgaata tcgaacgttt cccgaacggc     1620

aaaaagtacg agctggacaa gtacactatg ttccattacc tgcgtgcaca ggagtttgaa     1680

cacggtaaaa gccgtatcgc gctgaccaac agcgttaacg aggccctgct gaacccgagc     1740

cgtgtctata ccttcttcag cagcgactat gttaagaaag tgaacaaagc cactgaggcc     1800

gcgatgttcc tgggctgggt ggaacagctg gtatatgact tcacggacga gacgagcgaa     1860

gtgagcacta ccgacaaaat tgctgatatt accatcatta tcccgtatat tggtccggca     1920

ctgaacattg gcaacatgct gtacaaagac gattttgtgg gtgccctgat cttctccggt     1980

gccgtgattc tgctggagtt cattccggag attgcgatcc cggtgttggg taccttcgcg     2040

ctggtgtcct acatcgcgaa taaggttctg acggttcaga ccatcgataa cgcgctgtcg     2100

aaacgtaatg aaaaatggga cgaggtttac aaatacattg ttacgaattg gctggcgaaa     2160

gtcaataccc agatcgacct gatccgtaag aaaatgaaag aggcgctgga gaatcaggcg     2220

gaggccacca aagcaattat caactaccaa tacaaccagt acacggaaga agagaagaat     2280

aacattaact tcaatatcga tgatttgagc agcaagctga atgaatctat caacaaagcg     2340

atgatcaata tcaacaagtt tttgaatcag tgtagcgttt cgtacctgat gaatagcatg     2400

attccgtatg gcgtcaaacg tctggaggac ttcgacgcca gcctgaaaga tgcgttgctg     2460

aaatacattt acgacaatcg tggtacgctg attggccaag ttgaccgctt gaaagacaaa     2520

gttaacaata ccctgagcac cgacatccca tttcaactga gcaagtatgt tgataatcaa     2580

cgtctgttga gcactttcac cgagtatatc aaaaacatca tcaatactag cattctgaac     2640

ctgcgttacg agagcaatca tctgattgat ctgagccgtt atgcaagcaa gatcaacatc     2700

ggtagcaagg tcaattttga cccgatcgat aagaaccaga tccagctgtt taatctggaa     2760

tcgagcaaaa ttgaggttat cctgaaaaac gccattgtct acaactccat gtacgagaat     2820

ttctccacca gcttctggat tcgcatcccg aaatacttca acagcattag cctgaacaac     2880

gagtatacta tcatcaactg tatggagaac aacagcggtt ggaaggtgtc tctgaactat     2940

ggtgagatca tttggacctt gcaggacacc caagagatca agcagcgcgt cgtgttcaag     3000

tactctcaaa tgatcaacat ttccgattac attaatcgtt ggatcttcgt gaccattacg     3060

aataaccgtc tgaataacag caagatttac atcaatggtc gcttgatcga tcagaaaccg     3120

attagcaacc tgggtaatat ccacgcaagc aacaacatta tgttcaaatt ggacggttgc     3180

cgcgataccc atcgttatat ctggatcaag tatttcaacc tgtttgataa agaactgaat     3240

gagaaggaga tcaaagattt gtatgacaac caatctaaca gcggcatttt gaaggacttc     3300

tggggcgatt atctgcaata cgataagccg tactatatgc tgaacctgta tgatccgaac     3360

aaatatgtgg atgtcaataa tgtgggtatt cgtggttaca tgtatttgaa gggtccgcgt     3420

ggcagcgtta tgacgaccaa catttacctg aactctagcc tgtaccgtgg tacgaaattc     3480

atcattaaga aatatgccag cggcaacaaa gataacattg tgcgtaataa cgatcgtgtc     3540

tacatcaacg tggtcgtgaa gcgtaaagag taccgtctgg cgaccaacgc ttcgcaggcg     3600

ggtgttgaga aaattctgag cgcgttggag atccctcgtg tccgtcgtct gagccaagtc     3660

gtggttatga agagcaagaa cgaccagggt atcactaaca agtgcaagat gaacctgcaa     3720

gaccgtcgtg gtaacgacat cggctttatt ggtttccacc agttcaacaa tattgctaaa     3780

ctggtagcga gcaattggta caatcgtcag attgagcgcc gtagccgtcg tttgggctgt     3840

agctgggagt ttatcccggt cgatgatggt tggggcgaac gtccgctg                  3888


<210>  10
<211>  1296
<212>  PRT
<213>  artificial

<220>
<223>  Modified BoNT/A "Cat-D"

<400>  10

Met Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly 
1               5                   10                  15      


Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro 
            20                  25                  30          


Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg 
        35                  40                  45              


Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu 
    50                  55                  60                  


Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr 
65                  70                  75                  80  


Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu 
                85                  90                  95      


Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val 
            100                 105                 110         


Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys 
        115                 120                 125             


Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr 
    130                 135                 140                 


Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile 
145                 150                 155                 160 


Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr 
                165                 170                 175     


Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe 
            180                 185                 190         


Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu 
        195                 200                 205             


Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Glu 
    210                 215                 220                 


Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn 
225                 230                 235                 240 


Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu 
                245                 250                 255     


Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys 
            260                 265                 270         


Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn 
        275                 280                 285             


Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val 
    290                 295                 300                 


Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys 
305                 310                 315                 320 


Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu 
                325                 330                 335     


Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp 
            340                 345                 350         


Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu Asn 
        355                 360                 365             


Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr 
    370                 375                 380                 


Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn 
385                 390                 395                 400 


Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu 
                405                 410                 415     


Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Arg 
            420                 425                 430         


Gly Ile Ile Thr Ser Lys Thr Lys Ser Leu Asp Lys Gly Tyr Asn Lys 
        435                 440                 445             


Ala Leu Asn Asp Leu Cys Ile Lys Val Asn Asn Trp Asp Leu Phe Phe 
    450                 455                 460                 


Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys Gly Glu Glu 
465                 470                 475                 480 


Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu Asn Ile Ser Leu 
                485                 490                 495     


Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe Asp Asn Glu Pro 
            500                 505                 510         


Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile Ile Gly Gln Leu 
        515                 520                 525             


Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly Lys Lys Tyr Glu 
    530                 535                 540                 


Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala Gln Glu Phe Glu 
545                 550                 555                 560 


His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val Asn Glu Ala Leu 
                565                 570                 575     


Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp Tyr Val Lys 
            580                 585                 590         


Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu Gly Trp Val Glu 
        595                 600                 605             


Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu Val Ser Thr Thr 
    610                 615                 620                 


Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr Ile Gly Pro Ala 
625                 630                 635                 640 


Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe Val Gly Ala Leu 
                645                 650                 655     


Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile Pro Glu Ile Ala 
            660                 665                 670         


Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile Ala Asn Lys 
        675                 680                 685             


Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser Lys Arg Asn Glu 
    690                 695                 700                 


Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp Leu Ala Lys 
705                 710                 715                 720 


Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met Lys Glu Ala Leu 
                725                 730                 735     


Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn Tyr Gln Tyr Asn 
            740                 745                 750         


Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe Asn Ile Asp Asp 
        755                 760                 765             


Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala Met Ile Asn Ile 
    770                 775                 780                 


Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu Met Asn Ser Met 
785                 790                 795                 800 


Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp Ala Ser Leu Lys 
                805                 810                 815     


Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly Thr Leu Ile Gly 
            820                 825                 830         


Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr Leu Ser Thr Asp 
        835                 840                 845             


Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln Arg Leu Leu Ser 
    850                 855                 860                 


Thr Phe Thr Glu Tyr Ile Lys Asn Ile Ile Asn Thr Ser Ile Leu Asn 
865                 870                 875                 880 


Leu Arg Tyr Glu Ser Asn His Leu Ile Asp Leu Ser Arg Tyr Ala Ser 
                885                 890                 895     


Lys Ile Asn Ile Gly Ser Lys Val Asn Phe Asp Pro Ile Asp Lys Asn 
            900                 905                 910         


Gln Ile Gln Leu Phe Asn Leu Glu Ser Ser Lys Ile Glu Val Ile Leu 
        915                 920                 925             


Lys Asn Ala Ile Val Tyr Asn Ser Met Tyr Glu Asn Phe Ser Thr Ser 
    930                 935                 940                 


Phe Trp Ile Arg Ile Pro Lys Tyr Phe Asn Ser Ile Ser Leu Asn Asn 
945                 950                 955                 960 


Glu Tyr Thr Ile Ile Asn Cys Met Glu Asn Asn Ser Gly Trp Lys Val 
                965                 970                 975     


Ser Leu Asn Tyr Gly Glu Ile Ile Trp Thr Leu Gln Asp Thr Gln Glu 
            980                 985                 990         


Ile Lys Gln Arg Val Val Phe Lys  Tyr Ser Gln Met Ile  Asn Ile Ser 
        995                 1000                 1005             


Asp Tyr  Ile Asn Arg Trp Ile  Phe Val Thr Ile Thr  Asn Asn Arg 
    1010                 1015                 1020             


Leu Asn  Asn Ser Lys Ile Tyr  Ile Asn Gly Arg Leu  Ile Asp Gln 
    1025                 1030                 1035             


Lys Pro  Ile Ser Asn Leu Gly  Asn Ile His Ala Ser  Asn Asn Ile 
    1040                 1045                 1050             


Met Phe  Lys Leu Asp Gly Cys  Arg Asp Thr His Arg  Tyr Ile Trp 
    1055                 1060                 1065             


Ile Lys  Tyr Phe Asn Leu Phe  Asp Lys Glu Leu Asn  Glu Lys Glu 
    1070                 1075                 1080             


Ile Lys  Asp Leu Tyr Asp Asn  Gln Ser Asn Ser Gly  Ile Leu Lys 
    1085                 1090                 1095             


Asp Phe  Trp Gly Asp Tyr Leu  Gln Tyr Asp Lys Pro  Tyr Tyr Met 
    1100                 1105                 1110             


Leu Asn  Leu Tyr Asp Pro Asn  Lys Tyr Val Asp Val  Asn Asn Val 
    1115                 1120                 1125             


Gly Ile  Arg Gly Tyr Met Tyr  Leu Lys Gly Pro Arg  Gly Ser Val 
    1130                 1135                 1140             


Met Thr  Thr Asn Ile Tyr Leu  Asn Ser Ser Leu Tyr  Arg Gly Thr 
    1145                 1150                 1155             


Lys Phe  Ile Ile Lys Lys Tyr  Ala Ser Gly Asn Lys  Asp Asn Ile 
    1160                 1165                 1170             


Val Arg  Asn Asn Asp Arg Val  Tyr Ile Asn Val Val  Val Lys Arg 
    1175                 1180                 1185             


Lys Glu  Tyr Arg Leu Ala Thr  Asn Ala Ser Gln Ala  Gly Val Glu 
    1190                 1195                 1200             


Lys Ile  Leu Ser Ala Leu Glu  Ile Pro Arg Val Arg  Arg Leu Ser 
    1205                 1210                 1215             


Gln Val  Val Val Met Lys Ser  Lys Asn Asp Gln Gly  Ile Thr Asn 
    1220                 1225                 1230             


Lys Cys  Lys Met Asn Leu Gln  Asp Arg Arg Gly Asn  Asp Ile Gly 
    1235                 1240                 1245             


Phe Ile  Gly Phe His Gln Phe  Asn Asn Ile Ala Lys  Leu Val Ala 
    1250                 1255                 1260             


Ser Asn  Trp Tyr Asn Arg Gln  Ile Glu Arg Arg Ser  Arg Arg Leu 
    1265                 1270                 1275             


Gly Cys  Ser Trp Glu Phe Ile  Pro Val Asp Asp Gly  Trp Gly Glu 
    1280                 1285                 1290             


Arg Pro  Leu 
    1295     


<210>  11
<211>  1315
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Modified BoNT/A "Chimera 1"

<400>  11

Met Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly 
1               5                   10                  15      


Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro 
            20                  25                  30          


Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg 
        35                  40                  45              


Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu 
    50                  55                  60                  


Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr 
65                  70                  75                  80  


Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu 
                85                  90                  95      


Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val 
            100                 105                 110         


Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys 
        115                 120                 125             


Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr 
    130                 135                 140                 


Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile 
145                 150                 155                 160 


Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr 
                165                 170                 175     


Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe 
            180                 185                 190         


Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu 
        195                 200                 205             


Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Glu 
    210                 215                 220                 


Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn 
225                 230                 235                 240 


Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu 
                245                 250                 255     


Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys 
            260                 265                 270         


Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn 
        275                 280                 285             


Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val 
    290                 295                 300                 


Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys 
305                 310                 315                 320 


Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu 
                325                 330                 335     


Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp 
            340                 345                 350         


Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu Asn 
        355                 360                 365             


Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr 
    370                 375                 380                 


Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn 
385                 390                 395                 400 


Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu 
                405                 410                 415     


Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Arg 
            420                 425                 430         


Gly Ile Ile Thr Ser Lys Thr Lys Ser Leu Asp Lys Gly Tyr Asn Lys 
        435                 440                 445             


Ala Leu Asn Asp Leu Cys Ile Lys Val Asn Asn Trp Asp Leu Phe Phe 
    450                 455                 460                 


Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys Gly Glu Glu 
465                 470                 475                 480 


Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu Asn Ile Ser Leu 
                485                 490                 495     


Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe Asp Asn Glu Pro 
            500                 505                 510         


Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile Ile Gly Gln Leu 
        515                 520                 525             


Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly Lys Lys Tyr Glu 
    530                 535                 540                 


Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala Gln Glu Phe Glu 
545                 550                 555                 560 


His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val Asn Glu Ala Leu 
                565                 570                 575     


Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp Tyr Val Lys 
            580                 585                 590         


Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu Gly Trp Val Glu 
        595                 600                 605             


Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu Val Ser Thr Thr 
    610                 615                 620                 


Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr Ile Gly Pro Ala 
625                 630                 635                 640 


Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe Val Gly Ala Leu 
                645                 650                 655     


Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile Pro Glu Ile Ala 
            660                 665                 670         


Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile Ala Asn Lys 
        675                 680                 685             


Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser Lys Arg Asn Glu 
    690                 695                 700                 


Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp Leu Ala Lys 
705                 710                 715                 720 


Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met Lys Glu Ala Leu 
                725                 730                 735     


Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn Tyr Gln Tyr Asn 
            740                 745                 750         


Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe Asn Ile Asp Asp 
        755                 760                 765             


Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala Met Ile Asn Ile 
    770                 775                 780                 


Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu Met Asn Ser Met 
785                 790                 795                 800 


Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp Ala Ser Leu Lys 
                805                 810                 815     


Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly Thr Leu Ile Gly 
            820                 825                 830         


Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr Leu Ser Thr Asp 
        835                 840                 845             


Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln Arg Leu Leu Ser 
    850                 855                 860                 


Thr Phe Thr Glu Tyr Ile Lys Ser Glu Ile Leu Asn Asn Ile Ile Leu 
865                 870                 875                 880 


Asn Leu Arg Tyr Lys Asp Asn Asn Leu Ile Asp Leu Ser Gly Tyr Gly 
                885                 890                 895     


Ala Lys Val Glu Val Tyr Asp Gly Val Glu Leu Asn Asp Lys Asn Gln 
            900                 905                 910         


Phe Lys Leu Thr Ser Ser Ala Asn Ser Lys Ile Arg Val Thr Gln Asn 
        915                 920                 925             


Gln Asn Ile Ile Phe Asn Ser Val Phe Leu Asp Phe Ser Val Ser Phe 
    930                 935                 940                 


Trp Ile Arg Ile Pro Lys Tyr Lys Asn Asp Gly Ile Gln Asn Tyr Ile 
945                 950                 955                 960 


His Asn Glu Tyr Thr Ile Ile Asn Cys Met Lys Asn Asn Ser Gly Trp 
                965                 970                 975     


Lys Ile Ser Ile Arg Gly Asn Arg Ile Ile Trp Thr Leu Ile Asp Ile 
            980                 985                 990         


Asn Gly Lys Thr Lys Ser Val Phe  Phe Glu Tyr Asn Ile  Arg Glu Asp 
        995                 1000                 1005             


Ile Ser  Glu Tyr Ile Asn Arg  Trp Phe Phe Val Thr  Ile Thr Asn 
    1010                 1015                 1020             


Asn Leu  Asn Asn Ala Lys Ile  Tyr Ile Asn Gly Lys  Leu Glu Ser 
    1025                 1030                 1035             


Asn Thr  Asp Ile Lys Asp Ile  Arg Glu Val Ile Ala  Asn Gly Glu 
    1040                 1045                 1050             


Ile Ile  Phe Lys Leu Asp Gly  Asp Ile Asp Arg Thr  Gln Phe Ile 
    1055                 1060                 1065             


Trp Met  Lys Tyr Phe Ser Ile  Phe Asn Thr Glu Leu  Ser Gln Ser 
    1070                 1075                 1080             


Asn Ile  Glu Glu Arg Tyr Lys  Ile Gln Ser Tyr Ser  Glu Tyr Leu 
    1085                 1090                 1095             


Lys Asp  Phe Trp Gly Asn Pro  Leu Met Tyr Asn Lys  Glu Tyr Tyr 
    1100                 1105                 1110             


Met Phe  Asn Ala Gly Asn Lys  Asn Ser Tyr Ile Lys  Leu Lys Lys 
    1115                 1120                 1125             


Asp Ser  Pro Val Gly Glu Ile  Leu Thr Arg Ser Lys  Tyr Asn Gln 
    1130                 1135                 1140             


Asn Ser  Lys Tyr Ile Asn Tyr  Arg Asp Leu Tyr Ile  Gly Glu Lys 
    1145                 1150                 1155             


Phe Ile  Ile Arg Arg Lys Ser  Asn Ser Gln Ser Ile  Asn Asp Asp 
    1160                 1165                 1170             


Ile Val  Arg Lys Glu Asp Tyr  Ile Tyr Leu Asp Phe  Phe Asn Leu 
    1175                 1180                 1185             


Asn Gln  Glu Trp Arg Val Tyr  Thr Tyr Lys Tyr Phe  Lys Lys Glu 
    1190                 1195                 1200             


Glu Met  Lys Leu Phe Leu Ala  Pro Ile Tyr Asp Ser  Asp Glu Phe 
    1205                 1210                 1215             


Tyr Asn  Thr Ile Gln Ile Lys  Glu Tyr Asp Glu Gln  Pro Thr Tyr 
    1220                 1225                 1230             


Ser Cys  Gln Leu Leu Phe Lys  Lys Asp Glu Glu Ser  Thr Asp Glu 
    1235                 1240                 1245             


Ile Gly  Leu Ile Gly Ile His  Arg Phe Tyr Glu Ser  Gly Ile Val 
    1250                 1255                 1260             


Phe Glu  Glu Tyr Lys Asp Tyr  Phe Cys Ile Ser Lys  Trp Tyr Leu 
    1265                 1270                 1275             


Lys Glu  Val Lys Arg Lys Pro  Tyr Asn Leu Lys Leu  Gly Cys Asn 
    1280                 1285                 1290             


Trp Gln  Phe Ile Pro Lys Asp  Glu Gly Trp Thr Glu  His His His 
    1295                 1300                 1305             


His His  His His His His His  
    1310                 1315 


<210>  12
<211>  1327
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Modified BoNT/A "Chimera 2"

<400>  12

Met Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly 
1               5                   10                  15      


Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro 
            20                  25                  30          


Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg 
        35                  40                  45              


Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu 
    50                  55                  60                  


Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr 
65                  70                  75                  80  


Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu 
                85                  90                  95      


Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val 
            100                 105                 110         


Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys 
        115                 120                 125             


Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr 
    130                 135                 140                 


Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile 
145                 150                 155                 160 


Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr 
                165                 170                 175     


Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe 
            180                 185                 190         


Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu 
        195                 200                 205             


Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Glu 
    210                 215                 220                 


Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn 
225                 230                 235                 240 


Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu 
                245                 250                 255     


Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys 
            260                 265                 270         


Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn 
        275                 280                 285             


Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val 
    290                 295                 300                 


Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys 
305                 310                 315                 320 


Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu 
                325                 330                 335     


Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp 
            340                 345                 350         


Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu Asn 
        355                 360                 365             


Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr 
    370                 375                 380                 


Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn 
385                 390                 395                 400 


Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu 
                405                 410                 415     


Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Arg 
            420                 425                 430         


Gly Ile Ile Thr Ser Lys Thr Lys Ser Leu Asp Lys Gly Tyr Asn Lys 
        435                 440                 445             


Ala Leu Asn Asp Leu Cys Ile Lys Val Asn Asn Trp Asp Leu Phe Phe 
    450                 455                 460                 


Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys Gly Glu Glu 
465                 470                 475                 480 


Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu Asn Ile Ser Leu 
                485                 490                 495     


Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe Asp Asn Glu Pro 
            500                 505                 510         


Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile Ile Gly Gln Leu 
        515                 520                 525             


Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly Lys Lys Tyr Glu 
    530                 535                 540                 


Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala Gln Glu Phe Glu 
545                 550                 555                 560 


His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val Asn Glu Ala Leu 
                565                 570                 575     


Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp Tyr Val Lys 
            580                 585                 590         


Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu Gly Trp Val Glu 
        595                 600                 605             


Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu Val Ser Thr Thr 
    610                 615                 620                 


Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr Ile Gly Pro Ala 
625                 630                 635                 640 


Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe Val Gly Ala Leu 
                645                 650                 655     


Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile Pro Glu Ile Ala 
            660                 665                 670         


Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile Ala Asn Lys 
        675                 680                 685             


Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser Lys Arg Asn Glu 
    690                 695                 700                 


Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp Leu Ala Lys 
705                 710                 715                 720 


Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met Lys Glu Ala Leu 
                725                 730                 735     


Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn Tyr Gln Tyr Asn 
            740                 745                 750         


Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe Asn Ile Asp Asp 
        755                 760                 765             


Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala Met Ile Asn Ile 
    770                 775                 780                 


Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu Met Asn Ser Met 
785                 790                 795                 800 


Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp Ala Ser Leu Lys 
                805                 810                 815     


Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly Thr Leu Ile Gly 
            820                 825                 830         


Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr Leu Ser Thr Asp 
        835                 840                 845             


Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln Arg Leu Leu Ser 
    850                 855                 860                 


Thr Phe Thr Glu Tyr Ile Lys Asn Ile Ile Glu Leu Gly Gly Gly Gly 
865                 870                 875                 880 


Ser Glu Leu Ser Glu Ile Leu Asn Asn Ile Ile Leu Asn Leu Arg Tyr 
                885                 890                 895     


Lys Asp Asn Asn Leu Ile Asp Leu Ser Gly Tyr Gly Ala Lys Val Glu 
            900                 905                 910         


Val Tyr Asp Gly Val Glu Leu Asn Asp Lys Asn Gln Phe Lys Leu Thr 
        915                 920                 925             


Ser Ser Ala Asn Ser Lys Ile Arg Val Thr Gln Asn Gln Asn Ile Ile 
    930                 935                 940                 


Phe Asn Ser Val Phe Leu Asp Phe Ser Val Ser Phe Trp Ile Arg Ile 
945                 950                 955                 960 


Pro Lys Tyr Lys Asn Asp Gly Ile Gln Asn Tyr Ile His Asn Glu Tyr 
                965                 970                 975     


Thr Ile Ile Asn Cys Met Lys Asn Asn Ser Gly Trp Lys Ile Ser Ile 
            980                 985                 990         


Arg Gly Asn Arg Ile Ile Trp Thr  Leu Ile Asp Ile Asn  Gly Lys Thr 
        995                 1000                 1005             


Lys Ser  Val Phe Phe Glu Tyr  Asn Ile Arg Glu Asp  Ile Ser Glu 
    1010                 1015                 1020             


Tyr Ile  Asn Arg Trp Phe Phe  Val Thr Ile Thr Asn  Asn Leu Asn 
    1025                 1030                 1035             


Asn Ala  Lys Ile Tyr Ile Asn  Gly Lys Leu Glu Ser  Asn Thr Asp 
    1040                 1045                 1050             


Ile Lys  Asp Ile Arg Glu Val  Ile Ala Asn Gly Glu  Ile Ile Phe 
    1055                 1060                 1065             


Lys Leu  Asp Gly Asp Ile Asp  Arg Thr Gln Phe Ile  Trp Met Lys 
    1070                 1075                 1080             


Tyr Phe  Ser Ile Phe Asn Thr  Glu Leu Ser Gln Ser  Asn Ile Glu 
    1085                 1090                 1095             


Glu Arg  Tyr Lys Ile Gln Ser  Tyr Ser Glu Tyr Leu  Lys Asp Phe 
    1100                 1105                 1110             


Trp Gly  Asn Pro Leu Met Tyr  Asn Lys Glu Tyr Tyr  Met Phe Asn 
    1115                 1120                 1125             


Ala Gly  Asn Lys Asn Ser Tyr  Ile Lys Leu Lys Lys  Asp Ser Pro 
    1130                 1135                 1140             


Val Gly  Glu Ile Leu Thr Arg  Ser Lys Tyr Asn Gln  Asn Ser Lys 
    1145                 1150                 1155             


Tyr Ile  Asn Tyr Arg Asp Leu  Tyr Ile Gly Glu Lys  Phe Ile Ile 
    1160                 1165                 1170             


Arg Arg  Lys Ser Asn Ser Gln  Ser Ile Asn Asp Asp  Ile Val Arg 
    1175                 1180                 1185             


Lys Glu  Asp Tyr Ile Tyr Leu  Asp Phe Phe Asn Leu  Asn Gln Glu 
    1190                 1195                 1200             


Trp Arg  Val Tyr Thr Tyr Lys  Tyr Phe Lys Lys Glu  Glu Met Lys 
    1205                 1210                 1215             


Leu Phe  Leu Ala Pro Ile Tyr  Asp Ser Asp Glu Phe  Tyr Asn Thr 
    1220                 1225                 1230             


Ile Gln  Ile Lys Glu Tyr Asp  Glu Gln Pro Thr Tyr  Ser Cys Gln 
    1235                 1240                 1245             


Leu Leu  Phe Lys Lys Asp Glu  Glu Ser Thr Asp Glu  Ile Gly Leu 
    1250                 1255                 1260             


Ile Gly  Ile His Arg Phe Tyr  Glu Ser Gly Ile Val  Phe Glu Glu 
    1265                 1270                 1275             


Tyr Lys  Asp Tyr Phe Cys Ile  Ser Lys Trp Tyr Leu  Lys Glu Val 
    1280                 1285                 1290             


Lys Arg  Lys Pro Tyr Asn Leu  Lys Leu Gly Cys Asn  Trp Gln Phe 
    1295                 1300                 1305             


Ile Pro  Lys Asp Glu Gly Trp  Thr Glu His His His  His His His 
    1310                 1315                 1320             


His His  His His 
    1325         


<210>  13
<211>  1314
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Modified BoNT/A "Chimera 3A"

<400>  13

Met Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly 
1               5                   10                  15      


Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro 
            20                  25                  30          


Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg 
        35                  40                  45              


Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu 
    50                  55                  60                  


Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr 
65                  70                  75                  80  


Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu 
                85                  90                  95      


Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val 
            100                 105                 110         


Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys 
        115                 120                 125             


Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr 
    130                 135                 140                 


Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile 
145                 150                 155                 160 


Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr 
                165                 170                 175     


Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe 
            180                 185                 190         


Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu 
        195                 200                 205             


Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Glu 
    210                 215                 220                 


Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn 
225                 230                 235                 240 


Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu 
                245                 250                 255     


Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys 
            260                 265                 270         


Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn 
        275                 280                 285             


Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val 
    290                 295                 300                 


Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys 
305                 310                 315                 320 


Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu 
                325                 330                 335     


Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp 
            340                 345                 350         


Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu Asn 
        355                 360                 365             


Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr 
    370                 375                 380                 


Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn 
385                 390                 395                 400 


Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu 
                405                 410                 415     


Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Arg 
            420                 425                 430         


Gly Ile Ile Thr Ser Lys Thr Lys Ser Leu Asp Lys Gly Tyr Asn Lys 
        435                 440                 445             


Ala Leu Asn Asp Leu Cys Ile Lys Val Asn Asn Trp Asp Leu Phe Phe 
    450                 455                 460                 


Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys Gly Glu Glu 
465                 470                 475                 480 


Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu Asn Ile Ser Leu 
                485                 490                 495     


Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe Asp Asn Glu Pro 
            500                 505                 510         


Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile Ile Gly Gln Leu 
        515                 520                 525             


Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly Lys Lys Tyr Glu 
    530                 535                 540                 


Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala Gln Glu Phe Glu 
545                 550                 555                 560 


His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val Asn Glu Ala Leu 
                565                 570                 575     


Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp Tyr Val Lys 
            580                 585                 590         


Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu Gly Trp Val Glu 
        595                 600                 605             


Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu Val Ser Thr Thr 
    610                 615                 620                 


Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr Ile Gly Pro Ala 
625                 630                 635                 640 


Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe Val Gly Ala Leu 
                645                 650                 655     


Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile Pro Glu Ile Ala 
            660                 665                 670         


Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile Ala Asn Lys 
        675                 680                 685             


Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser Lys Arg Asn Glu 
    690                 695                 700                 


Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp Leu Ala Lys 
705                 710                 715                 720 


Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met Lys Glu Ala Leu 
                725                 730                 735     


Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn Tyr Gln Tyr Asn 
            740                 745                 750         


Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe Asn Ile Asp Asp 
        755                 760                 765             


Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala Met Ile Asn Ile 
    770                 775                 780                 


Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu Met Asn Ser Met 
785                 790                 795                 800 


Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp Ala Ser Leu Lys 
                805                 810                 815     


Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly Thr Leu Ile Gly 
            820                 825                 830         


Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr Leu Ser Thr Asp 
        835                 840                 845             


Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln Arg Leu Leu Ser 
    850                 855                 860                 


Thr Phe Thr Glu Tyr Ile Lys Asn Ile Leu Asn Asn Ile Ile Leu Asn 
865                 870                 875                 880 


Leu Arg Tyr Lys Asp Asn Asn Leu Ile Asp Leu Ser Gly Tyr Gly Ala 
                885                 890                 895     


Lys Val Glu Val Tyr Asp Gly Val Glu Leu Asn Asp Lys Asn Gln Phe 
            900                 905                 910         


Lys Leu Thr Ser Ser Ala Asn Ser Lys Ile Arg Val Thr Gln Asn Gln 
        915                 920                 925             


Asn Ile Ile Phe Asn Ser Val Phe Leu Asp Phe Ser Val Ser Phe Trp 
    930                 935                 940                 


Ile Arg Ile Pro Lys Tyr Lys Asn Asp Gly Ile Gln Asn Tyr Ile His 
945                 950                 955                 960 


Asn Glu Tyr Thr Ile Ile Asn Cys Met Lys Asn Asn Ser Gly Trp Lys 
                965                 970                 975     


Ile Ser Ile Arg Gly Asn Arg Ile Ile Trp Thr Leu Ile Asp Ile Asn 
            980                 985                 990         


Gly Lys Thr Lys Ser Val Phe Phe  Glu Tyr Asn Ile Arg  Glu Asp Ile 
        995                 1000                 1005             


Ser Glu  Tyr Ile Asn Arg Trp  Phe Phe Val Thr Ile  Thr Asn Asn 
    1010                 1015                 1020             


Leu Asn  Asn Ala Lys Ile Tyr  Ile Asn Gly Lys Leu  Glu Ser Asn 
    1025                 1030                 1035             


Thr Asp  Ile Lys Asp Ile Arg  Glu Val Ile Ala Asn  Gly Glu Ile 
    1040                 1045                 1050             


Ile Phe  Lys Leu Asp Gly Asp  Ile Asp Arg Thr Gln  Phe Ile Trp 
    1055                 1060                 1065             


Met Lys  Tyr Phe Ser Ile Phe  Asn Thr Glu Leu Ser  Gln Ser Asn 
    1070                 1075                 1080             


Ile Glu  Glu Arg Tyr Lys Ile  Gln Ser Tyr Ser Glu  Tyr Leu Lys 
    1085                 1090                 1095             


Asp Phe  Trp Gly Asn Pro Leu  Met Tyr Asn Lys Glu  Tyr Tyr Met 
    1100                 1105                 1110             


Phe Asn  Ala Gly Asn Lys Asn  Ser Tyr Ile Lys Leu  Lys Lys Asp 
    1115                 1120                 1125             


Ser Pro  Val Gly Glu Ile Leu  Thr Arg Ser Lys Tyr  Asn Gln Asn 
    1130                 1135                 1140             


Ser Lys  Tyr Ile Asn Tyr Arg  Asp Leu Tyr Ile Gly  Glu Lys Phe 
    1145                 1150                 1155             


Ile Ile  Arg Arg Lys Ser Asn  Ser Gln Ser Ile Asn  Asp Asp Ile 
    1160                 1165                 1170             


Val Arg  Lys Glu Asp Tyr Ile  Tyr Leu Asp Phe Phe  Asn Leu Asn 
    1175                 1180                 1185             


Gln Glu  Trp Arg Val Tyr Thr  Tyr Lys Tyr Phe Lys  Lys Glu Glu 
    1190                 1195                 1200             


Met Lys  Leu Phe Leu Ala Pro  Ile Tyr Asp Ser Asp  Glu Phe Tyr 
    1205                 1210                 1215             


Asn Thr  Ile Gln Ile Lys Glu  Tyr Asp Glu Gln Pro  Thr Tyr Ser 
    1220                 1225                 1230             


Cys Gln  Leu Leu Phe Lys Lys  Asp Glu Glu Ser Thr  Asp Glu Ile 
    1235                 1240                 1245             


Gly Leu  Ile Gly Ile His Arg  Phe Tyr Glu Ser Gly  Ile Val Phe 
    1250                 1255                 1260             


Glu Glu  Tyr Lys Asp Tyr Phe  Cys Ile Ser Lys Trp  Tyr Leu Lys 
    1265                 1270                 1275             


Glu Val  Lys Arg Lys Pro Tyr  Asn Leu Lys Leu Gly  Cys Asn Trp 
    1280                 1285                 1290             


Gln Phe  Ile Pro Lys Asp Glu  Gly Trp Thr Glu His  His His His 
    1295                 1300                 1305             


His His  His His His His 
    1310                 


<210>  14
<211>  1304
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Modified BoNT/A "Chimera 3B"

<400>  14

Met Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly 
1               5                   10                  15      


Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro 
            20                  25                  30          


Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg 
        35                  40                  45              


Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu 
    50                  55                  60                  


Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr 
65                  70                  75                  80  


Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu 
                85                  90                  95      


Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val 
            100                 105                 110         


Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys 
        115                 120                 125             


Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr 
    130                 135                 140                 


Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile 
145                 150                 155                 160 


Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr 
                165                 170                 175     


Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe 
            180                 185                 190         


Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu 
        195                 200                 205             


Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Glu 
    210                 215                 220                 


Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn 
225                 230                 235                 240 


Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu 
                245                 250                 255     


Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys 
            260                 265                 270         


Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn 
        275                 280                 285             


Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val 
    290                 295                 300                 


Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys 
305                 310                 315                 320 


Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu 
                325                 330                 335     


Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp 
            340                 345                 350         


Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu Asn 
        355                 360                 365             


Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr 
    370                 375                 380                 


Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn 
385                 390                 395                 400 


Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu 
                405                 410                 415     


Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Arg 
            420                 425                 430         


Gly Ile Ile Thr Ser Lys Thr Lys Ser Leu Asp Lys Gly Tyr Asn Lys 
        435                 440                 445             


Ala Leu Asn Asp Leu Cys Ile Lys Val Asn Asn Trp Asp Leu Phe Phe 
    450                 455                 460                 


Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys Gly Glu Glu 
465                 470                 475                 480 


Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu Asn Ile Ser Leu 
                485                 490                 495     


Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe Asp Asn Glu Pro 
            500                 505                 510         


Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile Ile Gly Gln Leu 
        515                 520                 525             


Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly Lys Lys Tyr Glu 
    530                 535                 540                 


Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala Gln Glu Phe Glu 
545                 550                 555                 560 


His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val Asn Glu Ala Leu 
                565                 570                 575     


Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp Tyr Val Lys 
            580                 585                 590         


Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu Gly Trp Val Glu 
        595                 600                 605             


Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu Val Ser Thr Thr 
    610                 615                 620                 


Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr Ile Gly Pro Ala 
625                 630                 635                 640 


Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe Val Gly Ala Leu 
                645                 650                 655     


Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile Pro Glu Ile Ala 
            660                 665                 670         


Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile Ala Asn Lys 
        675                 680                 685             


Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser Lys Arg Asn Glu 
    690                 695                 700                 


Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp Leu Ala Lys 
705                 710                 715                 720 


Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met Lys Glu Ala Leu 
                725                 730                 735     


Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn Tyr Gln Tyr Asn 
            740                 745                 750         


Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe Asn Ile Asp Asp 
        755                 760                 765             


Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala Met Ile Asn Ile 
    770                 775                 780                 


Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu Met Asn Ser Met 
785                 790                 795                 800 


Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp Ala Ser Leu Lys 
                805                 810                 815     


Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly Thr Leu Ile Gly 
            820                 825                 830         


Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr Leu Ser Thr Asp 
        835                 840                 845             


Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln Arg Leu Leu Ser 
    850                 855                 860                 


Thr Phe Thr Glu Tyr Ile Lys Asn Ile Leu Asn Asn Ile Ile Leu Asn 
865                 870                 875                 880 


Leu Arg Tyr Lys Asp Asn Asn Leu Ile Asp Leu Ser Gly Tyr Gly Ala 
                885                 890                 895     


Lys Val Glu Val Tyr Asp Gly Val Glu Leu Asn Asp Lys Asn Gln Phe 
            900                 905                 910         


Lys Leu Thr Ser Ser Ala Asn Ser Lys Ile Arg Val Thr Gln Asn Gln 
        915                 920                 925             


Asn Ile Ile Phe Asn Ser Val Phe Leu Asp Phe Ser Val Ser Phe Trp 
    930                 935                 940                 


Ile Arg Ile Pro Lys Tyr Lys Asn Asp Gly Ile Gln Asn Tyr Ile His 
945                 950                 955                 960 


Asn Glu Tyr Thr Ile Ile Asn Cys Met Lys Asn Asn Ser Gly Trp Lys 
                965                 970                 975     


Ile Ser Ile Arg Gly Asn Arg Ile Ile Trp Thr Leu Ile Asp Ile Asn 
            980                 985                 990         


Gly Lys Thr Lys Ser Val Phe Phe  Glu Tyr Asn Ile Arg  Glu Asp Ile 
        995                 1000                 1005             


Ser Glu  Tyr Ile Asn Arg Trp  Phe Phe Val Thr Ile  Thr Asn Asn 
    1010                 1015                 1020             


Leu Asn  Asn Ala Lys Ile Tyr  Ile Asn Gly Lys Leu  Glu Ser Asn 
    1025                 1030                 1035             


Thr Asp  Ile Lys Asp Ile Arg  Glu Val Ile Ala Asn  Gly Glu Ile 
    1040                 1045                 1050             


Ile Phe  Lys Leu Asp Gly Asp  Ile Asp Arg Thr Gln  Phe Ile Trp 
    1055                 1060                 1065             


Met Lys  Tyr Phe Ser Ile Phe  Asn Thr Glu Leu Ser  Gln Ser Asn 
    1070                 1075                 1080             


Ile Glu  Glu Arg Tyr Lys Ile  Gln Ser Tyr Ser Glu  Tyr Leu Lys 
    1085                 1090                 1095             


Asp Phe  Trp Gly Asn Pro Leu  Met Tyr Asn Lys Glu  Tyr Tyr Met 
    1100                 1105                 1110             


Phe Asn  Ala Gly Asn Lys Asn  Ser Tyr Ile Lys Leu  Lys Lys Asp 
    1115                 1120                 1125             


Ser Pro  Val Gly Glu Ile Leu  Thr Arg Ser Lys Tyr  Asn Gln Asn 
    1130                 1135                 1140             


Ser Lys  Tyr Ile Asn Tyr Arg  Asp Leu Tyr Ile Gly  Glu Lys Phe 
    1145                 1150                 1155             


Ile Ile  Arg Arg Lys Ser Asn  Ser Gln Ser Ile Asn  Asp Asp Ile 
    1160                 1165                 1170             


Val Arg  Lys Glu Asp Tyr Ile  Tyr Leu Asp Phe Phe  Asn Leu Asn 
    1175                 1180                 1185             


Gln Glu  Trp Arg Val Tyr Thr  Tyr Lys Tyr Phe Lys  Lys Glu Glu 
    1190                 1195                 1200             


Met Lys  Leu Phe Leu Ala Pro  Ile Tyr Asp Ser Asp  Glu Phe Tyr 
    1205                 1210                 1215             


Asn Thr  Ile Gln Ile Lys Glu  Tyr Asp Glu Gln Pro  Thr Tyr Ser 
    1220                 1225                 1230             


Cys Gln  Leu Leu Phe Lys Lys  Asp Glu Glu Ser Thr  Asp Glu Ile 
    1235                 1240                 1245             


Gly Leu  Ile Gly Ile His Arg  Phe Tyr Glu Ser Gly  Ile Val Phe 
    1250                 1255                 1260             


Glu Glu  Tyr Lys Asp Tyr Phe  Cys Ile Ser Lys Trp  Tyr Leu Lys 
    1265                 1270                 1275             


Glu Val  Lys Arg Lys Pro Tyr  Asn Leu Lys Leu Gly  Cys Asn Trp 
    1280                 1285                 1290             


Gln Phe  Ile Pro Lys Asp Glu  Gly Trp Thr Glu 
    1295                 1300                 


<210>  15
<211>  1304
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Modified BoNT/A "Chimera 3C"

<400>  15

Met Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly 
1               5                   10                  15      


Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro 
            20                  25                  30          


Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg 
        35                  40                  45              


Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu 
    50                  55                  60                  


Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr 
65                  70                  75                  80  


Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu 
                85                  90                  95      


Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val 
            100                 105                 110         


Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys 
        115                 120                 125             


Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr 
    130                 135                 140                 


Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile 
145                 150                 155                 160 


Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr 
                165                 170                 175     


Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe 
            180                 185                 190         


Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu 
        195                 200                 205             


Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Glu 
    210                 215                 220                 


Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn 
225                 230                 235                 240 


Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu 
                245                 250                 255     


Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys 
            260                 265                 270         


Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn 
        275                 280                 285             


Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val 
    290                 295                 300                 


Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys 
305                 310                 315                 320 


Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu 
                325                 330                 335     


Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp 
            340                 345                 350         


Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu Asn 
        355                 360                 365             


Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr 
    370                 375                 380                 


Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn 
385                 390                 395                 400 


Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu 
                405                 410                 415     


Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Arg 
            420                 425                 430         


Gly Ile Ile Thr Ser Lys Thr Lys Ser Leu Asp Lys Gly Tyr Asn Lys 
        435                 440                 445             


Ala Leu Asn Asp Leu Cys Ile Lys Val Asn Asn Trp Asp Leu Phe Phe 
    450                 455                 460                 


Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys Gly Glu Glu 
465                 470                 475                 480 


Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu Asn Ile Ser Leu 
                485                 490                 495     


Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe Asp Asn Glu Pro 
            500                 505                 510         


Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile Ile Gly Gln Leu 
        515                 520                 525             


Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly Lys Lys Tyr Glu 
    530                 535                 540                 


Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala Gln Glu Phe Glu 
545                 550                 555                 560 


His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val Asn Glu Ala Leu 
                565                 570                 575     


Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp Tyr Val Lys 
            580                 585                 590         


Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu Gly Trp Val Glu 
        595                 600                 605             


Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu Val Ser Thr Thr 
    610                 615                 620                 


Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr Ile Gly Pro Ala 
625                 630                 635                 640 


Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe Val Gly Ala Leu 
                645                 650                 655     


Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile Pro Glu Ile Ala 
            660                 665                 670         


Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile Ala Asn Lys 
        675                 680                 685             


Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser Lys Arg Asn Glu 
    690                 695                 700                 


Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp Leu Ala Lys 
705                 710                 715                 720 


Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met Lys Glu Ala Leu 
                725                 730                 735     


Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn Tyr Gln Tyr Asn 
            740                 745                 750         


Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe Asn Ile Asp Asp 
        755                 760                 765             


Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala Met Ile Asn Ile 
    770                 775                 780                 


Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu Met Asn Ser Met 
785                 790                 795                 800 


Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp Ala Ser Leu Lys 
                805                 810                 815     


Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly Thr Leu Ile Gly 
            820                 825                 830         


Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr Leu Ser Thr Asp 
        835                 840                 845             


Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln Arg Leu Leu Ser 
    850                 855                 860                 


Thr Phe Thr Glu Tyr Ile Lys Asn Ile Leu Asn Asn Ile Ile Leu Asn 
865                 870                 875                 880 


Leu Arg Tyr Lys Asp Asn Asn Leu Ile Asp Leu Ser Gly Tyr Gly Ala 
                885                 890                 895     


Lys Val Glu Val Tyr Asp Gly Val Glu Leu Asn Asp Lys Asn Gln Phe 
            900                 905                 910         


Lys Leu Thr Ser Ser Ala Asn Ser Lys Ile Arg Val Thr Gln Asn Gln 
        915                 920                 925             


Asn Ile Ile Phe Asn Ser Val Phe Leu Asp Phe Ser Val Ser Phe Trp 
    930                 935                 940                 


Ile Arg Ile Pro Lys Tyr Lys Asn Asp Gly Ile Gln Asn Tyr Ile His 
945                 950                 955                 960 


Asn Glu Tyr Thr Ile Ile Asn Cys Met Lys Asn Asn Ser Gly Trp Lys 
                965                 970                 975     


Ile Ser Ile Arg Gly Asn Arg Ile Ile Trp Thr Leu Ile Asp Ile Asn 
            980                 985                 990         


Gly Lys Thr Lys Ser Val Phe Phe  Glu Tyr Asn Ile Arg  Glu Asp Ile 
        995                 1000                 1005             


Ser Glu  Tyr Ile Asn Arg Trp  Phe Phe Val Thr Ile  Thr Asn Asn 
    1010                 1015                 1020             


Leu Asn  Asn Ala Lys Ile Tyr  Ile Asn Gly Lys Leu  Glu Ser Asn 
    1025                 1030                 1035             


Thr Asp  Ile Lys Asp Ile Arg  Glu Val Ile Ala Asn  Gly Glu Ile 
    1040                 1045                 1050             


Ile Phe  Lys Leu Asp Gly Asp  Ile Asp Arg Thr Gln  Phe Ile Trp 
    1055                 1060                 1065             


Met Lys  Tyr Phe Ser Ile Phe  Asn Thr Glu Leu Ser  Gln Ser Asn 
    1070                 1075                 1080             


Ile Glu  Glu Arg Tyr Lys Ile  Gln Ser Tyr Ser Glu  Tyr Leu Lys 
    1085                 1090                 1095             


Asp Phe  Trp Gly Asn Pro Leu  Met Tyr Asn Lys Glu  Tyr Tyr Met 
    1100                 1105                 1110             


Phe Asn  Ala Gly Asn Lys Asn  Ser Tyr Ile Lys Leu  Lys Lys Asp 
    1115                 1120                 1125             


Ser Pro  Val Gly Glu Ile Leu  Thr Arg Ser Lys Tyr  Asn Gln Asn 
    1130                 1135                 1140             


Ser Lys  Tyr Ile Asn Tyr Arg  Asp Leu Tyr Ile Gly  Glu Lys Phe 
    1145                 1150                 1155             


Ile Ile  Arg Arg Lys Ser Asn  Ser Gln Ser Ile Asn  Asp Asp Ile 
    1160                 1165                 1170             


Val Arg  Lys Glu Asp Tyr Ile  Tyr Leu Asp Phe Phe  Asn Leu Asn 
    1175                 1180                 1185             


Gln Glu  Trp Arg Val Tyr Thr  Tyr Lys Tyr Phe Lys  Lys Glu Glu 
    1190                 1195                 1200             


Glu Lys  Leu Phe Leu Ala Pro  Ile Ser Asp Ser Asp  Glu Phe Tyr 
    1205                 1210                 1215             


Asn Thr  Ile Gln Ile Lys Glu  Tyr Asp Glu Gln Pro  Thr Tyr Ser 
    1220                 1225                 1230             


Cys Gln  Leu Leu Phe Lys Lys  Asp Glu Glu Ser Thr  Asp Glu Ile 
    1235                 1240                 1245             


Gly Leu  Ile Gly Ile His Arg  Phe Tyr Glu Ser Gly  Ile Val Phe 
    1250                 1255                 1260             


Glu Glu  Tyr Lys Asp Tyr Phe  Cys Ile Ser Lys Trp  Tyr Leu Lys 
    1265                 1270                 1275             


Glu Val  Lys Arg Lys Pro Tyr  Asn Leu Lys Leu Gly  Cys Asn Trp 
    1280                 1285                 1290             


Gln Phe  Ile Pro Lys Asp Glu  Gly Trp Thr Glu 
    1295                 1300                 


<210>  16
<211>  1291
<212>  PRT
<213>  Clostridium botulinum

<400>  16

Met Pro Val Thr Ile Asn Asn Phe Asn Tyr Asn Asp Pro Ile Asp Asn 
1               5                   10                  15      


Asn Asn Ile Ile Met Met Glu Pro Pro Phe Ala Arg Gly Thr Gly Arg 
            20                  25                  30          


Tyr Tyr Lys Ala Phe Lys Ile Thr Asp Arg Ile Trp Ile Ile Pro Glu 
        35                  40                  45              


Arg Tyr Thr Phe Gly Tyr Lys Pro Glu Asp Phe Asn Lys Ser Ser Gly 
    50                  55                  60                  


Ile Phe Asn Arg Asp Val Cys Glu Tyr Tyr Asp Pro Asp Tyr Leu Asn 
65                  70                  75                  80  


Thr Asn Asp Lys Lys Asn Ile Phe Leu Gln Thr Met Ile Lys Leu Phe 
                85                  90                  95      


Asn Arg Ile Lys Ser Lys Pro Leu Gly Glu Lys Leu Leu Glu Met Ile 
            100                 105                 110         


Ile Asn Gly Ile Pro Tyr Leu Gly Asp Arg Arg Val Pro Leu Glu Glu 
        115                 120                 125             


Phe Asn Thr Asn Ile Ala Ser Val Thr Val Asn Lys Leu Ile Ser Asn 
    130                 135                 140                 


Pro Gly Glu Val Glu Arg Lys Lys Gly Ile Phe Ala Asn Leu Ile Ile 
145                 150                 155                 160 


Phe Gly Pro Gly Pro Val Leu Asn Glu Asn Glu Thr Ile Asp Ile Gly 
                165                 170                 175     


Ile Gln Asn His Phe Ala Ser Arg Glu Gly Phe Gly Gly Ile Met Gln 
            180                 185                 190         


Met Lys Phe Cys Pro Glu Tyr Val Ser Val Phe Asn Asn Val Gln Glu 
        195                 200                 205             


Asn Lys Gly Ala Ser Ile Phe Asn Arg Arg Gly Tyr Phe Ser Asp Pro 
    210                 215                 220                 


Ala Leu Ile Leu Met His Glu Leu Ile His Val Leu His Gly Leu Tyr 
225                 230                 235                 240 


Gly Ile Lys Val Asp Asp Leu Pro Ile Val Pro Asn Glu Lys Lys Phe 
                245                 250                 255     


Phe Met Gln Ser Thr Asp Ala Ile Gln Ala Glu Glu Leu Tyr Thr Phe 
            260                 265                 270         


Gly Gly Gln Asp Pro Ser Ile Ile Thr Pro Ser Thr Asp Lys Ser Ile 
        275                 280                 285             


Tyr Asp Lys Val Leu Gln Asn Phe Arg Gly Ile Val Asp Arg Leu Asn 
    290                 295                 300                 


Lys Val Leu Val Cys Ile Ser Asp Pro Asn Ile Asn Ile Asn Ile Tyr 
305                 310                 315                 320 


Lys Asn Lys Phe Lys Asp Lys Tyr Lys Phe Val Glu Asp Ser Glu Gly 
                325                 330                 335     


Lys Tyr Ser Ile Asp Val Glu Ser Phe Asp Lys Leu Tyr Lys Ser Leu 
            340                 345                 350         


Met Phe Gly Phe Thr Glu Thr Asn Ile Ala Glu Asn Tyr Lys Ile Lys 
        355                 360                 365             


Thr Arg Ala Ser Tyr Phe Ser Asp Ser Leu Pro Pro Val Lys Ile Lys 
    370                 375                 380                 


Asn Leu Leu Asp Asn Glu Ile Tyr Thr Ile Glu Glu Gly Phe Asn Ile 
385                 390                 395                 400 


Ser Asp Lys Asp Met Glu Lys Glu Tyr Arg Gly Gln Asn Lys Ala Ile 
                405                 410                 415     


Asn Lys Gln Ala Tyr Glu Glu Ile Ser Lys Glu His Leu Ala Val Tyr 
            420                 425                 430         


Lys Ile Gln Met Cys Lys Ser Val Lys Ala Pro Gly Ile Cys Ile Asp 
        435                 440                 445             


Val Asp Asn Glu Asp Leu Phe Phe Ile Ala Asp Lys Asn Ser Phe Ser 
    450                 455                 460                 


Asp Asp Leu Ser Lys Asn Glu Arg Ile Glu Tyr Asn Thr Gln Ser Asn 
465                 470                 475                 480 


Tyr Ile Glu Asn Asp Phe Pro Ile Asn Glu Leu Ile Leu Asp Thr Asp 
                485                 490                 495     


Leu Ile Ser Lys Ile Glu Leu Pro Ser Glu Asn Thr Glu Ser Leu Thr 
            500                 505                 510         


Asp Phe Asn Val Asp Val Pro Val Tyr Glu Lys Gln Pro Ala Ile Lys 
        515                 520                 525             


Lys Ile Phe Thr Asp Glu Asn Thr Ile Phe Gln Tyr Leu Tyr Ser Gln 
    530                 535                 540                 


Thr Phe Pro Leu Asp Ile Arg Asp Ile Ser Leu Thr Ser Ser Phe Asp 
545                 550                 555                 560 


Asp Ala Leu Leu Phe Ser Asn Lys Val Tyr Ser Phe Phe Ser Met Asp 
                565                 570                 575     


Tyr Ile Lys Thr Ala Asn Lys Val Val Glu Ala Gly Leu Phe Ala Gly 
            580                 585                 590         


Trp Val Lys Gln Ile Val Asn Asp Phe Val Ile Glu Ala Asn Lys Ser 
        595                 600                 605             


Asn Thr Met Asp Lys Ile Ala Asp Ile Ser Leu Ile Val Pro Tyr Ile 
    610                 615                 620                 


Gly Leu Ala Leu Asn Val Gly Asn Glu Thr Ala Lys Gly Asn Phe Glu 
625                 630                 635                 640 


Asn Ala Phe Glu Ile Ala Gly Ala Ser Ile Leu Leu Glu Phe Ile Pro 
                645                 650                 655     


Glu Leu Leu Ile Pro Val Val Gly Ala Phe Leu Leu Glu Ser Tyr Ile 
            660                 665                 670         


Asp Asn Lys Asn Lys Ile Ile Lys Thr Ile Asp Asn Ala Leu Thr Lys 
        675                 680                 685             


Arg Asn Glu Lys Trp Ser Asp Met Tyr Gly Leu Ile Val Ala Gln Trp 
    690                 695                 700                 


Leu Ser Thr Val Asn Thr Gln Phe Tyr Thr Ile Lys Glu Gly Met Tyr 
705                 710                 715                 720 


Lys Ala Leu Asn Tyr Gln Ala Gln Ala Leu Glu Glu Ile Ile Lys Tyr 
                725                 730                 735     


Arg Tyr Asn Ile Tyr Ser Glu Lys Glu Lys Ser Asn Ile Asn Ile Asp 
            740                 745                 750         


Phe Asn Asp Ile Asn Ser Lys Leu Asn Glu Gly Ile Asn Gln Ala Ile 
        755                 760                 765             


Asp Asn Ile Asn Asn Phe Ile Asn Gly Cys Ser Val Ser Tyr Leu Met 
    770                 775                 780                 


Lys Lys Met Ile Pro Leu Ala Val Glu Lys Leu Leu Asp Phe Asp Asn 
785                 790                 795                 800 


Thr Leu Lys Lys Asn Leu Leu Asn Tyr Ile Asp Glu Asn Lys Leu Tyr 
                805                 810                 815     


Leu Ile Gly Ser Ala Glu Tyr Glu Lys Ser Lys Val Asn Lys Tyr Leu 
            820                 825                 830         


Lys Thr Ile Met Pro Phe Asp Leu Ser Ile Tyr Thr Asn Asp Thr Ile 
        835                 840                 845             


Leu Ile Glu Met Phe Asn Lys Tyr Asn Ser Glu Ile Leu Asn Asn Ile 
    850                 855                 860                 


Ile Leu Asn Leu Arg Tyr Lys Asp Asn Asn Leu Ile Asp Leu Ser Gly 
865                 870                 875                 880 


Tyr Gly Ala Lys Val Glu Val Tyr Asp Gly Val Glu Leu Asn Asp Lys 
                885                 890                 895     


Asn Gln Phe Lys Leu Thr Ser Ser Ala Asn Ser Lys Ile Arg Val Thr 
            900                 905                 910         


Gln Asn Gln Asn Ile Ile Phe Asn Ser Val Phe Leu Asp Phe Ser Val 
        915                 920                 925             


Ser Phe Trp Ile Arg Ile Pro Lys Tyr Lys Asn Asp Gly Ile Gln Asn 
    930                 935                 940                 


Tyr Ile His Asn Glu Tyr Thr Ile Ile Asn Cys Met Lys Asn Asn Ser 
945                 950                 955                 960 


Gly Trp Lys Ile Ser Ile Arg Gly Asn Arg Ile Ile Trp Thr Leu Ile 
                965                 970                 975     


Asp Ile Asn Gly Lys Thr Lys Ser Val Phe Phe Glu Tyr Asn Ile Arg 
            980                 985                 990         


Glu Asp Ile Ser Glu Tyr Ile Asn  Arg Trp Phe Phe Val  Thr Ile Thr 
        995                 1000                 1005             


Asn Asn  Leu Asn Asn Ala Lys  Ile Tyr Ile Asn Gly  Lys Leu Glu 
    1010                 1015                 1020             


Ser Asn  Thr Asp Ile Lys Asp  Ile Arg Glu Val Ile  Ala Asn Gly 
    1025                 1030                 1035             


Glu Ile  Ile Phe Lys Leu Asp  Gly Asp Ile Asp Arg  Thr Gln Phe 
    1040                 1045                 1050             


Ile Trp  Met Lys Tyr Phe Ser  Ile Phe Asn Thr Glu  Leu Ser Gln 
    1055                 1060                 1065             


Ser Asn  Ile Glu Glu Arg Tyr  Lys Ile Gln Ser Tyr  Ser Glu Tyr 
    1070                 1075                 1080             


Leu Lys  Asp Phe Trp Gly Asn  Pro Leu Met Tyr Asn  Lys Glu Tyr 
    1085                 1090                 1095             


Tyr Met  Phe Asn Ala Gly Asn  Lys Asn Ser Tyr Ile  Lys Leu Lys 
    1100                 1105                 1110             


Lys Asp  Ser Pro Val Gly Glu  Ile Leu Thr Arg Ser  Lys Tyr Asn 
    1115                 1120                 1125             


Gln Asn  Ser Lys Tyr Ile Asn  Tyr Arg Asp Leu Tyr  Ile Gly Glu 
    1130                 1135                 1140             


Lys Phe  Ile Ile Arg Arg Lys  Ser Asn Ser Gln Ser  Ile Asn Asp 
    1145                 1150                 1155             


Asp Ile  Val Arg Lys Glu Asp  Tyr Ile Tyr Leu Asp  Phe Phe Asn 
    1160                 1165                 1170             


Leu Asn  Gln Glu Trp Arg Val  Tyr Thr Tyr Lys Tyr  Phe Lys Lys 
    1175                 1180                 1185             


Glu Glu  Glu Lys Leu Phe Leu  Ala Pro Ile Ser Asp  Ser Asp Glu 
    1190                 1195                 1200             


Phe Tyr  Asn Thr Ile Gln Ile  Lys Glu Tyr Asp Glu  Gln Pro Thr 
    1205                 1210                 1215             


Tyr Ser  Cys Gln Leu Leu Phe  Lys Lys Asp Glu Glu  Ser Thr Asp 
    1220                 1225                 1230             


Glu Ile  Gly Leu Ile Gly Ile  His Arg Phe Tyr Glu  Ser Gly Ile 
    1235                 1240                 1245             


Val Phe  Glu Glu Tyr Lys Asp  Tyr Phe Cys Ile Ser  Lys Trp Tyr 
    1250                 1255                 1260             


Leu Lys  Glu Val Lys Arg Lys  Pro Tyr Asn Leu Lys  Leu Gly Cys 
    1265                 1270                 1275             


Asn Trp  Gln Phe Ile Pro Lys  Asp Glu Gly Trp Thr  Glu 
    1280                 1285                 1290     


