                         SEQUENCE LISTING

<110>  Universitt zu Kln
 
<120>  VEGF dimer molecules and columns comprising a VEGF dimer molecule
        as well as uses, production methods and methods involving the same

<130>  P72781PC

<160>  13    

<170>  PatentIn version 3.5

<210>  1
<211>  232
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Asn Phe Leu Leu Ser Trp Val His Trp Ser Leu Ala Leu Leu Leu 
1               5                   10                  15      


Tyr Leu His His Ala Lys Trp Ser Gln Ala Ala Pro Met Ala Glu Gly 
            20                  25                  30          


Gly Gly Gln Asn His His Glu Val Val Lys Phe Met Asp Val Tyr Gln 
        35                  40                  45              


Arg Ser Tyr Cys His Pro Ile Glu Thr Leu Val Asp Ile Phe Gln Glu 
    50                  55                  60                  


Tyr Pro Asp Glu Ile Glu Tyr Ile Phe Lys Pro Ser Cys Val Pro Leu 
65                  70                  75                  80  


Met Arg Cys Gly Gly Cys Cys Asn Asp Glu Gly Leu Glu Cys Val Pro 
                85                  90                  95      


Thr Glu Glu Ser Asn Ile Thr Met Gln Ile Met Arg Ile Lys Pro His 
            100                 105                 110         


Gln Gly Gln His Ile Gly Glu Met Ser Phe Leu Gln His Asn Lys Cys 
        115                 120                 125             


Glu Cys Arg Pro Lys Lys Asp Arg Ala Arg Gln Glu Lys Lys Ser Val 
    130                 135                 140                 


Arg Gly Lys Gly Lys Gly Gln Lys Arg Lys Arg Lys Lys Ser Arg Tyr 
145                 150                 155                 160 


Lys Ser Trp Ser Val Tyr Val Gly Ala Arg Cys Cys Leu Met Pro Trp 
                165                 170                 175     


Ser Leu Pro Gly Pro His Pro Cys Gly Pro Cys Ser Glu Arg Arg Lys 
            180                 185                 190         


His Leu Phe Val Gln Asp Pro Gln Thr Cys Lys Cys Ser Cys Lys Asn 
        195                 200                 205             


Thr Asp Ser Arg Cys Lys Ala Arg Gln Leu Glu Leu Asn Glu Arg Thr 
    210                 215                 220                 


Cys Arg Cys Asp Lys Pro Arg Arg 
225                 230         


<210>  2
<211>  26
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Asn Phe Leu Leu Ser Trp Val His Trp Ser Leu Ala Leu Leu Leu 
1               5                   10                  15      


Tyr Leu His His Ala Lys Trp Ser Gln Ala 
            20                  25      


<210>  3
<211>  167
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Example of a first VEGF molecule

<400>  3

Ala Ser Ala Pro Met Ala Glu Gly Gly Gly Gln Asn His His Glu Val 
1               5                   10                  15      


Val Lys Phe Met Asp Val Tyr Gln Arg Ser Tyr Cys His Pro Ile Glu 
            20                  25                  30          


Thr Leu Val Asp Ile Phe Gln Glu Tyr Pro Asp Glu Ile Glu Tyr Ile 
        35                  40                  45              


Phe Lys Pro Ser Cys Val Pro Leu Met Arg Cys Gly Gly Cys Cys Asn 
    50                  55                  60                  


Asp Glu Gly Leu Glu Cys Val Pro Thr Glu Glu Ser Asn Ile Thr Met 
65                  70                  75                  80  


Gln Ile Met Arg Ile Lys Pro His Gln Gly Gln His Ile Gly Glu Met 
                85                  90                  95      


Ser Phe Leu Gln His Asn Lys Cys Glu Cys Arg Pro Lys Lys Asp Arg 
            100                 105                 110         


Ala Arg Gln Glu Asn Pro Cys Gly Pro Cys Ser Glu Arg Arg Lys His 
        115                 120                 125             


Leu Phe Val Gln Asp Pro Gln Thr Cys Lys Cys Ser Cys Lys Asn Thr 
    130                 135                 140                 


Asp Ser Arg Cys Lys Ala Arg Gln Leu Glu Leu Asn Glu Arg Thr Cys 
145                 150                 155                 160 


Arg Cys Asp Lys Pro Arg Arg 
                165         


<210>  4
<211>  170
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Example of a second VEGF molecule

<400>  4

Thr Arg Ala Pro Met Ala Glu Gly Gly Gly Gln Asn His His Glu Val 
1               5                   10                  15      


Val Lys Phe Met Asp Val Tyr Gln Arg Ser Tyr Cys His Pro Ile Glu 
            20                  25                  30          


Thr Leu Val Asp Ile Phe Gln Glu Tyr Pro Asp Glu Ile Glu Tyr Ile 
        35                  40                  45              


Phe Lys Pro Ser Cys Val Pro Leu Met Arg Cys Gly Gly Cys Cys Asn 
    50                  55                  60                  


Asp Glu Gly Leu Glu Cys Val Pro Thr Glu Glu Ser Asn Ile Thr Met 
65                  70                  75                  80  


Gln Ile Met Arg Ile Lys Pro His Gln Gly Gln His Ile Gly Glu Met 
                85                  90                  95      


Ser Phe Leu Gln His Asn Lys Cys Glu Cys Arg Pro Lys Lys Asp Arg 
            100                 105                 110         


Ala Arg Gln Glu Asn Pro Cys Gly Pro Cys Ser Glu Arg Arg Lys His 
        115                 120                 125             


Leu Phe Val Gln Asp Pro Gln Thr Cys Lys Cys Ser Cys Lys Asn Thr 
    130                 135                 140                 


Asp Ser Arg Cys Lys Ala Arg Gln Leu Glu Leu Asn Glu Arg Thr Cys 
145                 150                 155                 160 


Arg Cys Asp Lys Pro Arg Arg Gly Ser Thr 
                165                 170 


<210>  5
<211>  351
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Example of a VEGF dimer molecule

<400>  5

Ala Ser Ala Pro Met Ala Glu Gly Gly Gly Gln Asn His His Glu Val 
1               5                   10                  15      


Val Lys Phe Met Asp Val Tyr Gln Arg Ser Tyr Cys His Pro Ile Glu 
            20                  25                  30          


Thr Leu Val Asp Ile Phe Gln Glu Tyr Pro Asp Glu Ile Glu Tyr Ile 
        35                  40                  45              


Phe Lys Pro Ser Cys Val Pro Leu Met Arg Cys Gly Gly Cys Cys Asn 
    50                  55                  60                  


Asp Glu Gly Leu Glu Cys Val Pro Thr Glu Glu Ser Asn Ile Thr Met 
65                  70                  75                  80  


Gln Ile Met Arg Ile Lys Pro His Gln Gly Gln His Ile Gly Glu Met 
                85                  90                  95      


Ser Phe Leu Gln His Asn Lys Cys Glu Cys Arg Pro Lys Lys Asp Arg 
            100                 105                 110         


Ala Arg Gln Glu Asn Pro Cys Gly Pro Cys Ser Glu Arg Arg Lys His 
        115                 120                 125             


Leu Phe Val Gln Asp Pro Gln Thr Cys Lys Cys Ser Cys Lys Asn Thr 
    130                 135                 140                 


Asp Ser Arg Cys Lys Ala Arg Gln Leu Glu Leu Asn Glu Arg Thr Cys 
145                 150                 155                 160 


Arg Cys Asp Lys Pro Arg Arg Gly Ser Thr Ser Gly Ser Gly Lys Ser 
                165                 170                 175     


Ser Glu Gly Lys Gly Thr Arg Ala Pro Met Ala Glu Gly Gly Gly Gln 
            180                 185                 190         


Asn His His Glu Val Val Lys Phe Met Asp Val Tyr Gln Arg Ser Tyr 
        195                 200                 205             


Cys His Pro Ile Glu Thr Leu Val Asp Ile Phe Gln Glu Tyr Pro Asp 
    210                 215                 220                 


Glu Ile Glu Tyr Ile Phe Lys Pro Ser Cys Val Pro Leu Met Arg Cys 
225                 230                 235                 240 


Gly Gly Cys Cys Asn Asp Glu Gly Leu Glu Cys Val Pro Thr Glu Glu 
                245                 250                 255     


Ser Asn Ile Thr Met Gln Ile Met Arg Ile Lys Pro His Gln Gly Gln 
            260                 265                 270         


His Ile Gly Glu Met Ser Phe Leu Gln His Asn Lys Cys Glu Cys Arg 
        275                 280                 285             


Pro Lys Lys Asp Arg Ala Arg Gln Glu Asn Pro Cys Gly Pro Cys Ser 
    290                 295                 300                 


Glu Arg Arg Lys His Leu Phe Val Gln Asp Pro Gln Thr Cys Lys Cys 
305                 310                 315                 320 


Ser Cys Lys Asn Thr Asp Ser Arg Cys Lys Ala Arg Gln Leu Glu Leu 
                325                 330                 335     


Asn Glu Arg Thr Cys Arg Cys Asp Lys Pro Arg Arg Gly Ser Thr 
            340                 345                 350     


<210>  6
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Example of a linker

<400>  6

Gly Ser Thr Ser Gly Ser Gly Lys Ser Ser Glu Gly Lys Gly 
1               5                   10                  


<210>  7
<211>  1053
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  scVEGF165 as used in the examples

<400>  7
gctagcgctc ctatggctga aggcggagga cagaatcacc acgaggtggt caagttcatg       60

gacgtgtacc agcggagcta ctgtcacccc atcgagacac tggtggacat cttccaagag      120

taccccgacg agatcgagta catcttcaag cccagctgcg tgcccctgat gagatgtggc      180

ggctgctgca atgacgaagg cctggaatgt gtgcccaccg aggaatccaa catcaccatg      240

cagatcatgc ggatcaagcc ccaccagggc cagcatatcg gcgagatgtc tttcctgcag      300

cacaacaagt gcgagtgcag acccaagaag gaccgggcca gacaagagaa tccttgcggc      360

ccttgcagcg agcggagaaa gcacctgttt gtgcaggacc ctcagacctg caagtgctcc      420

tgcaagaaca ccgacagccg gtgcaaagcc agacagctgg aactgaacga gcggacctgc      480

agatgcgaca agcctagaag aggcagcaca agcggcagcg gcaaaagctc tgaaggcaag      540

ggaacgcgtg ccccaatggc agaaggtggc ggccagaacc accatgaggt cgtgaagttt      600

atggatgtct atcagcggtc ctactgccat cctatcgaaa ccctggtcga tatttttcaa      660

gagtatccgg atgagattga gtatattttc aaaccctcct gtgtgccgct catgcgctgc      720

ggcggatgct gtaatgatga gggacttgag tgcgtgccaa ccgaagagtc taatattacg      780

atgcagatta tgagaatcaa accgcatcaa gggcagcata ttggggaaat gagcttcctc      840

cagcataaca aatgtgaatg ccggccgaag aaggacagag cccggcaaga aaacccatgc      900

ggcccctgtt ccgagaggcg gaaacatctg ttcgttcaag atccccagac ctgtaaatgt      960

agctgtaaaa acaccgactc caggtgcaag gcccggcaac tcgagctgaa cgagagaaca     1020

tgtcgctgcg ataagcccag acggggatcc aca                                  1053


<210>  8
<211>  846
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  scPIGF as used in the examples

<400>  8
acagctagcc tgcctgctgt tcctcctcaa caatgggccc tgtctgccgg caatggcagc       60

tctgaagttg aggtggtgcc cttccaagaa gtgtggggca gaagctactg cagagccctg      120

gaaagactgg tggacgtggt gtctgagtac cccagcgagg tggaacacat gttcagccct      180

agctgcgtgt ccctgctgag atgcacaggc tgttgcggcg acgagaatct gcactgcgtg      240

ccagtggaaa ccgccaacgt gacaatgcag ctgctgaaaa tcagaagcgg cgacagaccc      300

agctacgtgg aactgacctt cagccagcac gtccgctgcg agtgtagacc cctgcgggaa      360

aagatgaagc ccgagagatg cggagatgcc gtgcctagaa gaggcagcac atctggctct      420

ggcaagagca gcgaaggcaa gggacttcct gctgtgccac cacagcagtg ggcactgagt      480

gccggaaatg gctcctctga ggtggaagtc gtgccttttc aagaagtctg gggacgctcc      540

tactgtcgcg ctcttgagag actggtcgat gtcgtcagcg agtacccctc cgaagtcgag      600

cacatgtttt ccccatcctg tgtgtctctg ctgcggtgta ccggatgctg cggggatgag      660

aacctgcatt gtgtgcctgt cgagacagcc aatgtcacca tgcagctcct caagatcaga      720

tccggcgatc ggccctccta cgtcgagctg acattttctc agcacgttcg atgcgaatgc      780

cggcctctgc gcgagaaaat gaagcctgaa cgctgtggcg acgccgtgcc tcgtagagga      840

tccaca                                                                 846


<210>  9
<211>  282
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein sequence of scPIGF as used in the examples

<400>  9

Thr Ala Ser Leu Pro Ala Val Pro Pro Gln Gln Trp Ala Leu Ser Ala 
1               5                   10                  15      


Gly Asn Gly Ser Ser Glu Val Glu Val Val Pro Phe Gln Glu Val Trp 
            20                  25                  30          


Gly Arg Ser Tyr Cys Arg Ala Leu Glu Arg Leu Val Asp Val Val Ser 
        35                  40                  45              


Glu Tyr Pro Ser Glu Val Glu His Met Phe Ser Pro Ser Cys Val Ser 
    50                  55                  60                  


Leu Leu Arg Cys Thr Gly Cys Cys Gly Asp Glu Asn Leu His Cys Val 
65                  70                  75                  80  


Pro Val Glu Thr Ala Asn Val Thr Met Gln Leu Leu Lys Ile Arg Ser 
                85                  90                  95      


Gly Asp Arg Pro Ser Tyr Val Glu Leu Thr Phe Ser Gln His Val Arg 
            100                 105                 110         


Cys Glu Cys Arg Pro Leu Arg Glu Lys Met Lys Pro Glu Arg Cys Gly 
        115                 120                 125             


Asp Ala Val Pro Arg Arg Gly Ser Thr Ser Gly Ser Gly Lys Ser Ser 
    130                 135                 140                 


Glu Gly Lys Gly Leu Pro Ala Val Pro Pro Gln Gln Trp Ala Leu Ser 
145                 150                 155                 160 


Ala Gly Asn Gly Ser Ser Glu Val Glu Val Val Pro Phe Gln Glu Val 
                165                 170                 175     


Trp Gly Arg Ser Tyr Cys Arg Ala Leu Glu Arg Leu Val Asp Val Val 
            180                 185                 190         


Ser Glu Tyr Pro Ser Glu Val Glu His Met Phe Ser Pro Ser Cys Val 
        195                 200                 205             


Ser Leu Leu Arg Cys Thr Gly Cys Cys Gly Asp Glu Asn Leu His Cys 
    210                 215                 220                 


Val Pro Val Glu Thr Ala Asn Val Thr Met Gln Leu Leu Lys Ile Arg 
225                 230                 235                 240 


Ser Gly Asp Arg Pro Ser Tyr Val Glu Leu Thr Phe Ser Gln His Val 
                245                 250                 255     


Arg Cys Glu Cys Arg Pro Leu Arg Glu Lys Met Lys Pro Glu Arg Cys 
            260                 265                 270         


Gly Asp Ala Val Pro Arg Arg Gly Ser Thr 
        275                 280         


<210>  10
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer scVEGF165 forward

<400>  10
acagctagcg ctcctatggc tgaaggcgg                                         29


<210>  11
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer scVEGF165 reverse

<400>  11
tgtggatccc cgtctgggct tatcgcagc                                         29


<210>  12
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer scPlGF forward

<400>  12
acagctagcc tgcctgctgt tcctcctc                                          28


<210>  13
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer scPlGF reverse

<400>  13
tgtggatccc tctacgaggc acggcgtcg                                         29


