                               SEQUENCE LISTING

<110> THE JOHNS HOPKINS UNIVERSITY
      CAPRICOR, INC.
      GOULD, STEPHEN JOHN
      MARBAN, LINDA
      TSAI, SHANG-JUI
      GUO, CHENXU
 
<120> SELECTABLE MARKER PROTEINS, EXPRESSION VECTORS, ENGINEERED CELLS 
      AND EXTRACELLULAR VESICLES FOR THE PRODUCTION OF VIRUS-LIKE 
      PARTICLES FOR THERAPEUTIC AND PROPHYLACTIC APPLICATIONS

<130> JHU4250-1WO

<140> PCT/US2021/022200
<141> 2021-03-12

<150> 63/155,268
<151> 2021-03-01

<150> 63/110,325
<151> 2020-11-05

<150> 63/108,847
<151> 2020-11-02

<150> 63/061,766
<151> 2020-08-05

<150> 63/000,211
<151> 2020-03-26

<150> 62/990,946
<151> 2020-03-17

<150> 62/989,525
<151> 2020-03-13

<160> 13    

<170> PatentIn version 3.5

<210> 1
<211> 200
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 1
Met Thr Ser Asn Thr Pro Ala Val Arg Pro Ala Thr Arg Asp Asp Leu 
1               5                   10                  15      


Pro Arg Ala Leu Arg Thr Leu Gln Arg Ala Phe Ala Asp Tyr Pro Phe 
            20                  25                  30          


Thr Arg His Thr Ile Ala Ala Asp Asp His Leu Ala Arg Leu His Arg 
        35                  40                  45              


Phe Asn Glu Leu Phe Val Ser Arg Ile Gly Leu Asp His Gly Lys Val 
    50                  55                  60                  


Trp Val Ala Asp Asp Gly Asp Ala Val Ala Val Trp Thr Thr Pro Glu 
65                  70                  75                  80  


Thr Ala Asp Ala Gly Asn Val Phe Ala Glu Ile Gly Pro Gln Phe Ala 
                85                  90                  95      


Glu Ile Ala Gly Asp Arg Ala Asp Phe Ser Ala Gln Ala Glu Ala Ala 
            100                 105                 110         


Met Gly Pro His Arg Pro Thr Glu Pro Val Trp Phe Leu Gly Ser Val 
        115                 120                 125             


Gly Val Asp Pro Gly Arg Gln Gly Gln Gly Leu Gly Gly Ala Val Ile 
    130                 135                 140                 


Arg Pro Gly Leu Glu Ala Ala Glu Gln Ala Gly Val Pro Ala Phe Leu 
145                 150                 155                 160 


Glu Thr Ser Asp Glu Arg Asn Val Arg Phe Tyr Glu Arg Leu Gly Phe 
                165                 170                 175     


Glu Val Thr Ala Asp Tyr Pro Leu Pro Gly Gly Gly Pro Arg Thr Trp 
            180                 185                 190         


Ala Met Thr Arg Lys Pro Gly Ala 
        195                 200 


<210> 2
<211> 297
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 2
Met Arg Lys Pro Ile Val Ser Arg Ser Ser Leu Thr Ala Thr Ile Thr 
1               5                   10                  15      


Arg Ala Leu Gly Glu Val Ser Asp Leu Thr Gln Ile Thr Glu Gly Glu 
            20                  25                  30          


Glu Ser Arg Ala Phe Ser Phe Arg Ala Asn Gly Glu Asn Tyr Ile Val 
        35                  40                  45              


Arg Ile Asn Glu Thr Val Asn Gly Phe Asn Lys Asp Ala Tyr Ala Tyr 
    50                  55                  60                  


Gln Arg Phe Ala Thr Ala Ala Leu Pro Ile Pro Glu Val Val Ala Leu 
65                  70                  75                  80  


Gly Glu Leu Asp Asn Gly His Ala Tyr Cys Val Ser Arg Arg Ala Leu 
                85                  90                  95      


Gly Val Thr Leu Gln Asp Leu Thr Arg Thr Glu Leu Pro Ala Val Val 
            100                 105                 110         


Gly Pro Val Ala Ser Val Leu Glu Ala Ile Ala Ser Ser Thr Ile Gly 
        115                 120                 125             


Thr Ala Ser Gly Tyr Gly Pro Phe Asp Ser Gln Gly Arg Gly Ala Tyr 
    130                 135                 140                 


Ala Thr Trp Arg Asp Phe Leu Thr Ala Ile Ala Asn Pro His Gln Tyr 
145                 150                 155                 160 


Lys Trp Asn Thr Leu Arg His Gln Val Asp Val Asn Arg Ile Cys Leu 
                165                 170                 175     


Leu Leu Asn Glu Val Leu Tyr Leu Ala Glu Gln Cys Pro Glu Val Arg 
            180                 185                 190         


Gln Leu Val His Gly Asp Phe Gly Ser Asn Asn Val Leu Thr Asp Gly 
        195                 200                 205             


His Arg Ile Thr Gly Val Ile Asp Trp Ser Glu Ala Met Val Gly Asp 
    210                 215                 220                 


Pro Leu Tyr Asp Val Ala Asn Ile Leu Phe Trp Arg Thr Trp Leu Glu 
225                 230                 235                 240 


Cys Met Glu Gln Gln Ala Arg Phe Phe Glu Val His Cys Ala Asp His 
                245                 250                 255     


Leu Arg Pro Lys Glu Arg Leu Arg Cys Tyr Gln Leu Arg Ile Gly Leu 
            260                 265                 270         


Glu Glu Ile Tyr Glu Asn Ala Leu His Gly Thr Ala Asp Asn Val Ala 
        275                 280                 285             


Trp Ala Ile Asn Arg Cys Glu Glu Leu 
    290                 295         


<210> 3
<211> 264
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 3
Met Leu Glu Lys Asp Lys Phe Thr Thr Gly Ser Pro Ala Ala Trp Lys 
1               5                   10                  15      


Val Thr Leu Ala Gly Tyr Arg Trp Ile Gln Gln Thr Ile Gly Cys Ser 
            20                  25                  30          


Glu Ala Thr Val Phe Arg Leu Asp Ala Leu Gly Lys Pro Thr Leu Phe 
        35                  40                  45              


Val Lys Thr Glu Pro Ala Ser Pro Leu Ser Glu Leu Gln Asp Glu Ala 
    50                  55                  60                  


Ala Arg Leu Arg Trp Leu Ala Thr Val Gly Leu Ser Cys Ala Gln Val 
65                  70                  75                  80  


Leu Asp Ser Ala Asn Glu Ala Gly Arg Asp Trp Leu Leu Leu Asn Val 
                85                  90                  95      


Val Pro Gly Glu Asn Leu Leu Leu Ala Ser Leu Asp Pro Val Asp Lys 
            100                 105                 110         


Val Thr Ile Met Ala Asp Ala Leu Arg Arg Leu His Gln Leu Asp Pro 
        115                 120                 125             


Gly Thr Cys Pro Phe Asp His Arg Val Arg His Arg Ile Glu Arg Ala 
    130                 135                 140                 


Arg Asp Arg Ile Glu Ala Gly Leu Val Asp Gln Asp Asp Leu Asp Glu 
145                 150                 155                 160 


Glu His Gln Gly Leu Glu Pro Ala Lys Leu Phe Ala Arg Leu Arg Ala 
                165                 170                 175     


His Lys Pro Thr Thr Glu Asp Leu Val Val Thr His Gly Asp Ala Cys 
            180                 185                 190         


Leu Pro Asn Ile Met Val Glu Asn Gly Arg Phe Ser Gly Phe Ile Asp 
        195                 200                 205             


Cys Gly Arg Leu Gly Val Ala Asp Arg Tyr Gln Asp Leu Ala Leu Ala 
    210                 215                 220                 


Thr Arg Asp Ile Ala Glu Glu Leu Gly Asp Glu Trp Ile Lys Pro Leu 
225                 230                 235                 240 


Leu Val Gln Tyr Gly Ile Asn Asp Leu Asp Pro Asp Arg Thr Ala Phe 
                245                 250                 255     


Tyr Arg Leu Leu Asp Glu Phe Tyr 
            260                 


<210> 4
<211> 136
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 4
Gln Ile Thr Leu Thr Asp Lys Val Thr Ser Lys Asp Gln Asp Glu Leu 
1               5                   10                  15      


Arg Leu Gly Leu Asn Ala His Asn Ser Lys Phe Phe Asp Val Asp Leu 
            20                  25                  30          


Ile Lys Pro Leu Gly Leu Phe Ile Cys Asp Ser Gln Gly Lys Lys Leu 
        35                  40                  45              


Ala Gly Leu Thr Gly Thr Thr Thr Gly Asn Trp Leu Arg Ile Asp Leu 
    50                  55                  60                  


Leu Trp Val Ser Asp Ser Leu Arg Gly Gln Gly Thr Gly Ser Gln Leu 
65                  70                  75                  80  


Val Leu Ala Ala Glu Lys Glu Ala Arg Gln Arg Gly Cys Arg Phe Ala 
                85                  90                  95      


Gln Val Asp Thr Ala Ser Phe Gln Ala Arg Pro Phe Tyr Glu Lys Leu 
            100                 105                 110         


Gly Tyr His Val Arg Leu Thr Leu Gly Asp Tyr Ile His His His Gln 
        115                 120                 125             


Arg His Tyr Leu Thr Lys Ile Leu 
    130                 135     


<210> 5
<211> 128
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 5
Met Pro Leu Thr Thr Asp Glu Thr Ala Leu Val Asp Ala Ala Thr Ser 
1               5                   10                  15      


Thr Ile Thr Ser Ile Pro Ile Ser Asp Thr Tyr Ser Val Ala Ser Ala 
            20                  25                  30          


Ala Arg Ser Ser Asp Gly Arg Ile Phe Thr Gly Val Asn Val Phe His 
        35                  40                  45              


Phe Thr Gly Gly Pro Cys Ala Glu Leu Val Val Leu Gly Cys Ala Ala 
    50                  55                  60                  


Ala Ala Gly Ala Thr His Leu Thr His Ile Val Ala Val Gly Asn Glu 
65                  70                  75                  80  


Asn Arg Gly Ile Ile Ser Pro Cys Gly Arg Cys Arg Gln Thr Leu Ile 
                85                  90                  95      


Asp Leu His Pro Gly Ile Lys Val Val Val Leu Asp Arg Gly Glu Pro 
            100                 105                 110         


Arg Ala Val Ala Val Glu Glu Leu Leu Pro Phe Ala Tyr Leu Val Asp 
        115                 120                 125             


<210> 6
<211> 18
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 6
Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro 
1               5                   10                  15      


Gly Pro 
        


<210> 7
<211> 19
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 7
Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210> 8
<211> 20
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 8
Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp Val Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210> 9
<211> 22
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 9
Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val 
1               5                   10                  15      


Glu Ser Asn Pro Gly Pro 
            20          


<210> 10
<211> 67
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 10
ccaagcttat ctagaatcgg taccgcgatc gccgggttta aacacgcgtc tcgaggtcgg       60

atccgga                                                                 67


<210> 11
<211> 117
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 11
ccaagcttat ctagaatcgg taccgcgatc gccgggttta aacacgcgtc tcgagtaact       60

aactaacgga tcctaagtaa gtaagggccg gcctaaaata aaataattag cggccgc         117


<210> 12
<211> 199
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 12
Thr Ser Asn Thr Pro Ala Val Arg Pro Ala Thr Arg Asp Asp Leu Pro 
1               5                   10                  15      


Arg Ala Leu Arg Thr Leu Gln Arg Ala Phe Ala Asp Tyr Pro Phe Thr 
            20                  25                  30          


Arg His Thr Ile Ala Ala Asp Asp His Leu Ala Arg Leu His Arg Phe 
        35                  40                  45              


Asn Glu Leu Phe Val Ser Arg Ile Gly Leu Asp His Gly Lys Val Trp 
    50                  55                  60                  


Val Ala Asp Asp Gly Asp Ala Val Ala Val Trp Thr Thr Pro Glu Thr 
65                  70                  75                  80  


Ala Asp Ala Gly Asn Val Phe Ala Glu Ile Gly Pro Gln Phe Ala Glu 
                85                  90                  95      


Ile Ala Gly Asp Arg Ala Asp Phe Ser Ala Gln Ala Glu Ala Ala Met 
            100                 105                 110         


Gly Pro His Arg Pro Thr Glu Pro Val Trp Phe Leu Gly Ser Val Gly 
        115                 120                 125             


Val Asp Pro Gly Arg Gln Gly Gln Gly Leu Gly Gly Ala Val Ile Arg 
    130                 135                 140                 


Pro Gly Leu Glu Ala Ala Glu Gln Ala Gly Val Pro Ala Phe Leu Glu 
145                 150                 155                 160 


Thr Ser Asp Glu Arg Asn Val Arg Phe Tyr Glu Arg Leu Gly Phe Glu 
                165                 170                 175     


Val Thr Ala Asp Tyr Pro Leu Pro Gly Gly Gly Pro Arg Thr Trp Ala 
            180                 185                 190         


Met Thr Arg Lys Pro Gly Ala 
        195                 


<210> 13
<211> 198
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 13
Thr Glu Tyr Lys Pro Thr Val Arg Leu Ala Thr Arg Asp Asp Val Pro 
1               5                   10                  15      


Arg Ala Val Arg Thr Leu Ala Ala Ala Phe Ala Asp Tyr Pro Ala Thr 
            20                  25                  30          


Arg His Thr Val Asp Pro Asp Arg His Ile Glu Arg Val Thr Glu Leu 
        35                  40                  45              


Gln Glu Leu Phe Leu Thr Arg Val Gly Leu Asp Ile Gly Lys Val Trp 
    50                  55                  60                  


Val Ala Asp Asp Gly Ala Ala Val Ala Val Trp Thr Thr Pro Glu Ser 
65                  70                  75                  80  


Val Glu Ala Gly Ala Val Phe Ala Glu Ile Gly Pro Arg Met Ala Glu 
                85                  90                  95      


Leu Ser Gly Ser Arg Leu Ala Ala Gln Gln Gln Met Glu Gly Leu Leu 
            100                 105                 110         


Ala Pro His Arg Pro Lys Glu Pro Ala Trp Phe Leu Ala Thr Val Gly 
        115                 120                 125             


Val Ser Pro Asp His Gln Gly Lys Gly Leu Gly Ser Ala Val Val Leu 
    130                 135                 140                 


Pro Gly Val Glu Ala Ala Glu Arg Ala Gly Val Pro Ala Phe Leu Glu 
145                 150                 155                 160 


Thr Ser Ala Pro Arg Asn Leu Pro Phe Tyr Glu Arg Leu Gly Phe Thr 
                165                 170                 175     


Val Thr Ala Asp Val Glu Val Pro Glu Gly Pro Arg Thr Trp Cys Met 
            180                 185                 190         


Thr Arg Lys Pro Gly Ala 
        195             


