                         SEQUENCE LISTING

<110>  MASSACHUSETTS INSTITUTE OF TECHNOLOGY
        
<120>  COMPOSITIONS AND METHODS FOR IMMUNOTHERAPY OF NPM1c-POSITIVE 
       CANCER

<130>  MITN-057PC

<140>  PCT/US2021/012701
<141>  2021-01-08

<150>  US 62/987,612
<151>  2020-03-10

<160>  96    

<170>  PatentIn version 3.5

<210>  1
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Leukemia-specific neoantigen epitope of NPM1c

<400>  1

Ala Ile Gln Asp Leu Cys Leu Ala Val 
1               5                   


<210>  2
<211>  249
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: YG1 scFv amino acid sequence

<400>  2

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Ser Ile Ser Ser Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ala Ala Ser Ser Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Tyr Ser Thr Pro Leu 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Ser Gly Ile Leu Gly 
            100                 105                 110         


Thr Thr Ala Ala Ser Gly Ser Ser Gly Gly Ser Ser Ser Gly Ala Glu 
        115                 120                 125             


Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser 
    130                 135                 140                 


Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr Ala 
145                 150                 155                 160 


Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ser 
                165                 170                 175     


Ala Ile Ser Gly Ser Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val Lys 
            180                 185                 190         


Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu 
        195                 200                 205             


Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
    210                 215                 220                 


Arg Leu Gly Tyr Pro Thr Thr Thr Leu Leu Pro Phe Asp Tyr Trp Gly 
225                 230                 235                 240 


Gln Gly Thr Leu Val Thr Val Ser Ser 
                245                 


<210>  3
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: YG1 scFv VL amino acid sequence

<400>  3

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Ser Ile Ser Ser Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ala Ala Ser Ser Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Tyr Ser Thr Pro Leu 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Ser Gly Ile Leu Gly 
            100                 105                 110         


Thr Thr Ala Ala 
        115     


<210>  4
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: YG1 scFv linker amino acid sequence

<400>  4

Ser Gly Ser Ser Gly Gly Ser Ser Ser Gly 
1               5                   10  


<210>  5
<211>  123
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: YG1 scFv VH amino acid sequence

<400>  5

Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly 
1               5                   10                  15      


Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser 
            20                  25                  30          


Tyr Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp 
        35                  40                  45              


Val Ser Ala Ile Ser Gly Ser Gly Gly Ser Thr Tyr Tyr Ala Asp Ser 
    50                  55                  60                  


Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu 
65                  70                  75                  80  


Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr 
                85                  90                  95      


Cys Ala Arg Leu Gly Tyr Pro Thr Thr Thr Leu Leu Pro Phe Asp Tyr 
            100                 105                 110         


Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
        115                 120             


<210>  6
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: YG1 scFv VL CDR1 amino acid sequence (IMGT)

<400>  6

Gln Ser Ile Ser Ser Tyr 
1               5       


<210>  7
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: YG1 scFv VL CDR2 amino acid sequence (IMGT)

<400>  7

Ala Ala Ser 
1           


<210>  8
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: YG1 scFv VL CDR3 amino acid sequence (IMGT)

<400>  8

Gln Gln Ser Tyr Ser Thr Pro Leu Thr 
1               5                   


<210>  9
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: YG1 scFv VH CDR1 amino acid sequence (IMGT)

<400>  9

Gly Phe Thr Phe Ser Ser Tyr Ala 
1               5               


<210>  10
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: YG1 scFv VH CDR2 amino acid sequence (IMGT)

<400>  10

Ile Ser Gly Ser Gly Gly Ser Thr 
1               5               


<210>  11
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: YG1 scFv VH CDR3 amino acid sequence (IMGT)

<400>  11

Ala Arg Leu Gly Tyr Pro Thr Thr Thr Leu Leu Pro Phe Asp Tyr 
1               5                   10                  15  


<210>  12
<211>  747
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: YG1 scFv nucleic acid sequence

<400>  12
gacatccaga tgacccagtc tccatcctcc ctgtctgcat ctgtaggaga cagagtcacc       60

atcacttgcc gggcaagtca gagcattagc agctatttaa attggtatca gcagaaacca      120

gggaaagccc ctaagctcct gatctatgct gcatccagtt tgcaaagtgg ggtcccatca      180

aggttcagtg gcagtggatc tgggacagat ttcactctca ccatcagcag tctgcaacct      240

gaagattttg caacttacta ctgtcaacag agttacagta ccccgctcac gttcggccaa      300

gggaccaagg tggaaatcaa atccggaatt ctaggtacta ctgccgctag tggtagtagt      360

ggtggcagta gcagtggtgc cgaggtgcag ctggtggagt ctgggggagg cttggtacag      420

cctggggggt ccctgagact ctcctgtgca gcctctggat tcacctttag cagctatgcc      480

atgagctggg tccgccaggc tccagggaag gggctggagt gggtctcagc tattagtggt      540

agtggtggta gcacatacta cgcagactcc gtgaagggcc ggttcaccat ctccagagac      600

aattccaaga acacgctgta tctgcaaatg aacagcctga gagccgagga cacggccgtg      660

tattactgtg cgaggctggg ttaccctact actaccctac taccctttga ttactggggc      720

caaggtaccc tggtcactgt ctccagt                                          747


<210>  13
<211>  348
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: YG1 scFv VL nucleic acid sequence

<400>  13
gacatccaga tgacccagtc tccatcctcc ctgtctgcat ctgtaggaga cagagtcacc       60

atcacttgcc gggcaagtca gagcattagc agctatttaa attggtatca gcagaaacca      120

gggaaagccc ctaagctcct gatctatgct gcatccagtt tgcaaagtgg ggtcccatca      180

aggttcagtg gcagtggatc tgggacagat ttcactctca ccatcagcag tctgcaacct      240

gaagattttg caacttacta ctgtcaacag agttacagta ccccgctcac gttcggccaa      300

gggaccaagg tggaaatcaa atccggaatt ctaggtacta ctgccgct                   348


<210>  14
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: YG1 scFv linker nucleic acid sequence

<400>  14
agtggtagta gtggtggcag tagcagtggt                                        30


<210>  15
<211>  369
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: YG1 scFv VH nucleic acid sequence

<400>  15
gccgaggtgc agctggtgga gtctggggga ggcttggtac agcctggggg gtccctgaga       60

ctctcctgtg cagcctctgg attcaccttt agcagctatg ccatgagctg ggtccgccag      120

gctccaggga aggggctgga gtgggtctca gctattagtg gtagtggtgg tagcacatac      180

tacgcagact ccgtgaaggg ccggttcacc atctccagag acaattccaa gaacacgctg      240

tatctgcaaa tgaacagcct gagagccgag gacacggccg tgtattactg tgcgaggctg      300

ggttacccta ctactaccct actacccttt gattactggg gccaaggtac cctggtcact      360

gtctccagt                                                              369


<210>  16
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: YG1 scFv VL CDR1 nucleic acid sequence (IMGT)

<400>  16
cagagcatta gcagctat                                                     18


<210>  17
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: YG1 scFv VL CDR2 nucleic acid sequence (IMGT)

<400>  17
gctgcatcc                                                                9


<210>  18
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: YG1 scFv VL CDR3 nucleic acid sequence (IMGT)

<400>  18
caacagagtt acagtacccc gctcacg                                           27


<210>  19
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: YG1 scFv VH CDR1 nucleic acid sequence (IMGT)

<400>  19
ggattcacct ttagcagcta tgcc                                              24


<210>  20
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: YG1 scFv VH CDR2 nucleic acid sequence (IMGT)

<400>  20
attagtggta gtggtggtag caca                                              24


<210>  21
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: YG1 scFv VH CDR3 nucleic acid sequence (IMGT)

<400>  21
gcgaggctgg gttaccctac tactacccta ctaccctttg attac                       45


<210>  22
<211>  751
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: NPM1c CAR amino acid sequence (with YG1 scFv)

<400>  22

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln 
        35                  40                  45              


Ser Ile Ser Ser Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala 
    50                  55                  60                  


Pro Lys Leu Leu Ile Tyr Ala Ala Ser Ser Leu Gln Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile 
                85                  90                  95      


Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser 
            100                 105                 110         


Tyr Ser Thr Pro Leu Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
        115                 120                 125             


Ser Gly Ile Leu Gly Thr Thr Ala Ala Ser Gly Ser Ser Gly Gly Ser 
    130                 135                 140                 


Ser Ser Gly Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val 
145                 150                 155                 160 


Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr 
                165                 170                 175     


Phe Ser Ser Tyr Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly 
            180                 185                 190         


Leu Glu Trp Val Ser Ala Ile Ser Gly Ser Gly Gly Ser Thr Tyr Tyr 
        195                 200                 205             


Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys 
    210                 215                 220                 


Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
225                 230                 235                 240 


Val Tyr Tyr Cys Ala Arg Leu Gly Tyr Pro Thr Thr Thr Leu Leu Pro 
                245                 250                 255     


Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Thr Thr 
            260                 265                 270         


Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln 
        275                 280                 285             


Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala 
    290                 295                 300                 


Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala 
305                 310                 315                 320 


Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr 
                325                 330                 335     


Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln 
            340                 345                 350         


Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser 
        355                 360                 365             


Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys 
    370                 375                 380                 


Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly Gln Asn Gln 
385                 390                 395                 400 


Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu 
                405                 410                 415     


Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg 
            420                 425                 430         


Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
        435                 440                 445             


Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
    450                 455                 460                 


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
465                 470                 475                 480 


Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Ala Thr Asn 
                485                 490                 495     


Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro 
            500                 505                 510         


Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
        515                 520                 525             


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
    530                 535                 540                 


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
545                 550                 555                 560 


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
                565                 570                 575     


Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
            580                 585                 590         


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
        595                 600                 605             


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
    610                 615                 620                 


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
625                 630                 635                 640 


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
                645                 650                 655     


Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
            660                 665                 670         


Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
        675                 680                 685             


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
    690                 695                 700                 


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
705                 710                 715                 720 


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
                725                 730                 735     


Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
            740                 745                 750     


<210>  23
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Amino acid sequence of the leading sequence in the 
       NPM1c CAR

<400>  23

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro 
            20      


<210>  24
<211>  249
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Amino acid sequence of the YG1 scFv in the NPM1c CAR

<400>  24

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Ser Ile Ser Ser Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ala Ala Ser Ser Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Tyr Ser Thr Pro Leu 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Ser Gly Ile Leu Gly 
            100                 105                 110         


Thr Thr Ala Ala Ser Gly Ser Ser Gly Gly Ser Ser Ser Gly Ala Glu 
        115                 120                 125             


Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser 
    130                 135                 140                 


Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr Ala 
145                 150                 155                 160 


Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ser 
                165                 170                 175     


Ala Ile Ser Gly Ser Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val Lys 
            180                 185                 190         


Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu 
        195                 200                 205             


Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
    210                 215                 220                 


Arg Leu Gly Tyr Pro Thr Thr Thr Leu Leu Pro Phe Asp Tyr Trp Gly 
225                 230                 235                 240 


Gln Gly Thr Leu Val Thr Val Ser Ser 
                245                 


<210>  25
<211>  69
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Amino acid sequence of the CD8 hinge and transmembrane
       regions in the NPM1c CAR

<400>  25

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile 
        35                  40                  45              


Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val 
    50                  55                  60                  


Ile Thr Leu Tyr Cys 
65                  


<210>  26
<211>  42
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Amino acid sequence of the 4-1BB signaling domain in 
       the NPM1c CAR

<400>  26

Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40          


<210>  27
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Amino acid sequence of the CD3-zeta signaling domain 
       in the NPM1c CAR

<400>  27

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  28
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Amino acid sequence of the P2A self-cleaving peptide 
       in the NPM1c CAR

<400>  28

Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  29
<211>  239
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Amino acid sequence of the EGFP region in the NPM1c 
       CAR

<400>  29

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235                 


<210>  30
<211>  2265
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: NPM1c CAR nucleic acid sequence (with YG1 scFv)

<400>  30
atggccctcc ctgtcaccgc cctgctgctt ccgctggctc ttctgctcca cgccgctcgg       60

cccgacatcc agatgaccca gtctccatcc tccctgtctg catctgtagg agacagagtc      120

accatcactt gccgggcaag tcagagcatt agcagctatt taaattggta tcagcagaaa      180

ccagggaaag cccctaagct cctgatctat gctgcatcca gtttgcaaag tggggtccca      240

tcaaggttca gtggcagtgg atctgggaca gatttcactc tcaccatcag cagtctgcaa      300

cctgaagatt ttgcaactta ctactgtcaa cagagttaca gtaccccgct cacgttcggc      360

caagggacca aggtggaaat caaatccgga attctaggta ctactgccgc tagtggtagt      420

agtggtggca gtagcagtgg tgccgaggtg cagctggtgg agtctggggg aggcttggta      480

cagcctgggg ggtccctgag actctcctgt gcagcctctg gattcacctt tagcagctat      540

gccatgagct gggtccgcca ggctccaggg aaggggctgg agtgggtctc agctattagt      600

ggtagtggtg gtagcacata ctacgcagac tccgtgaagg gccggttcac catctccaga      660

gacaattcca agaacacgct gtatctgcaa atgaacagcc tgagagccga ggacacggcc      720

gtgtattact gtgcgaggct gggttaccct actactaccc tactaccctt tgattactgg      780

ggccaaggta ccctggtcac tgtctccagt accactaccc cagcaccgag gccacccacc      840

ccggctccta ccatcgcctc ccagcctctg tccctgcgtc cggaggcatg tagacccgca      900

gctggtgggg ccgtgcatac ccggggtctt gacttcgcct gcgatatcta catttgggcc      960

cctctggctg gtacttgcgg ggtcctgctg ctttcactcg tgatcactct ttactgtaag     1020

cgcggtcgga agaagctgct gtacatcttt aagcaaccct tcatgaggcc tgtgcagact     1080

actcaagagg aggacggctg ttcatgccgg ttcccagagg aggaggaagg cggctgcgaa     1140

ctgcgcgtga aattcagccg cagcgcagat gctccagcct acaagcaggg gcagaaccag     1200

ctctacaacg aactcaatct tggtcggaga gaggagtacg acgtgctgga caagcggaga     1260

ggacgggacc cagaaatggg cgggaagccg cgcagaaaga atccccaaga gggcctgtac     1320

aacgagctcc aaaaggataa gatggcagaa gcctatagcg agattggtat gaaaggggaa     1380

cgcagaagag gcaaaggcca cgacggactg taccagggac tcagcaccgc caccaaggac     1440

acctatgacg ctcttcacat gcaggccctg ccgcctcggg gatccggcgc aacaaacttc     1500

tctctgctga aacaagccgg agatgtcgaa gagaatcctg gaccgatggt gagcaagggc     1560

gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc     1620

cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg     1680

aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg     1740

acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc     1800

aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc     1860

aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag     1920

ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac     1980

tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac     2040

ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag     2100

aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag     2160

tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg     2220

accgccgccg ggatcactct cggcatggac gagctgtaca agtga                     2265


<210>  31
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Nucleic acid sequence of the leading sequence in the 
       NPM1c CAR

<400>  31
atggccctcc ctgtcaccgc cctgctgctt ccgctggctc ttctgctcca cgccgctcgg       60

ccc                                                                     63


<210>  32
<211>  747
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Nucleic acid sequence of the YG1 scFv in the NPM1c CAR

<400>  32
gacatccaga tgacccagtc tccatcctcc ctgtctgcat ctgtaggaga cagagtcacc       60

atcacttgcc gggcaagtca gagcattagc agctatttaa attggtatca gcagaaacca      120

gggaaagccc ctaagctcct gatctatgct gcatccagtt tgcaaagtgg ggtcccatca      180

aggttcagtg gcagtggatc tgggacagat ttcactctca ccatcagcag tctgcaacct      240

gaagattttg caacttacta ctgtcaacag agttacagta ccccgctcac gttcggccaa      300

gggaccaagg tggaaatcaa atccggaatt ctaggtacta ctgccgctag tggtagtagt      360

ggtggcagta gcagtggtgc cgaggtgcag ctggtggagt ctgggggagg cttggtacag      420

cctggggggt ccctgagact ctcctgtgca gcctctggat tcacctttag cagctatgcc      480

atgagctggg tccgccaggc tccagggaag gggctggagt gggtctcagc tattagtggt      540

agtggtggta gcacatacta cgcagactcc gtgaagggcc ggttcaccat ctccagagac      600

aattccaaga acacgctgta tctgcaaatg aacagcctga gagccgagga cacggccgtg      660

tattactgtg cgaggctggg ttaccctact actaccctac taccctttga ttactggggc      720

caaggtaccc tggtcactgt ctccagt                                          747


<210>  33
<211>  207
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Nucleic acid sequence of the CD8 hinge and 
       transmembrane regions in the NPM1c CAR

<400>  33
accactaccc cagcaccgag gccacccacc ccggctccta ccatcgcctc ccagcctctg       60

tccctgcgtc cggaggcatg tagacccgca gctggtgggg ccgtgcatac ccggggtctt      120

gacttcgcct gcgatatcta catttgggcc cctctggctg gtacttgcgg ggtcctgctg      180

ctttcactcg tgatcactct ttactgt                                          207


<210>  34
<211>  126
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Nucleic acid sequence of the 4-1BB signaling domain in
       the NPM1c CAR

<400>  34
aagcgcggtc ggaagaagct gctgtacatc tttaagcaac ccttcatgag gcctgtgcag       60

actactcaag aggaggacgg ctgttcatgc cggttcccag aggaggagga aggcggctgc      120

gaactg                                                                 126


<210>  35
<211>  336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Nucleic acid sequence of the CD3-zeta signaling domain
       in the NPM1c CAR

<400>  35
cgcgtgaaat tcagccgcag cgcagatgct ccagcctaca agcaggggca gaaccagctc       60

tacaacgaac tcaatcttgg tcggagagag gagtacgacg tgctggacaa gcggagagga      120

cgggacccag aaatgggcgg gaagccgcgc agaaagaatc cccaagaggg cctgtacaac      180

gagctccaaa aggataagat ggcagaagcc tatagcgaga ttggtatgaa aggggaacgc      240

agaagaggca aaggccacga cggactgtac cagggactca gcaccgccac caaggacacc      300

tatgacgctc ttcacatgca ggccctgccg cctcgg                                336


<210>  36
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Nucleic acid sequence of the P2A self-cleaving peptide
       in the NPM1c CAR

<400>  36
gcaacaaact tctctctgct gaaacaagcc ggagatgtcg aagagaatcc tggaccg          57


<210>  37
<211>  720
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Nucleic acid sequence of the EGFP region in the NPM1c 
       CAR

<400>  37
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac       60

ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac      120

ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc      180

ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag      240

cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc      300

ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg      360

gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac      420

aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac      480

ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc      540

gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac      600

tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc      660

ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtga      720


<210>  38
<211>  330
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(330)
<223>  Human IgG1

<400>  38

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
225                 230                 235                 240 


Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  39
<211>  326
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(326)
<223>  Human IgG4 (terminal K absent)

<400>  39

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro Ala Pro 
            100                 105                 110         


Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
        115                 120                 125             


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
    130                 135                 140                 


Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp 
145                 150                 155                 160 


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe 
                165                 170                 175     


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
            180                 185                 190         


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu 
        195                 200                 205             


Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
    210                 215                 220                 


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys 
225                 230                 235                 240 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
                245                 250                 255     


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
            260                 265                 270         


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
        275                 280                 285             


Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser 
    290                 295                 300                 


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
305                 310                 315                 320 


Leu Ser Leu Ser Leu Gly 
                325     


<210>  40
<211>  326
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(326)
<223>  Human IgG4 single mutant (S228P) (terminal K absent)

<400>  40

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro 
            100                 105                 110         


Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
        115                 120                 125             


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
    130                 135                 140                 


Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp 
145                 150                 155                 160 


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe 
                165                 170                 175     


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
            180                 185                 190         


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu 
        195                 200                 205             


Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
    210                 215                 220                 


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys 
225                 230                 235                 240 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
                245                 250                 255     


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
            260                 265                 270         


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
        275                 280                 285             


Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser 
    290                 295                 300                 


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
305                 310                 315                 320 


Leu Ser Leu Ser Leu Gly 
                325     


<210>  41
<211>  326
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(326)
<223>  Human IgG4 double mutant (S228P) (L235E) (terminal K absent)

<400>  41

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro 
            100                 105                 110         


Glu Phe Glu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
        115                 120                 125             


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
    130                 135                 140                 


Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp 
145                 150                 155                 160 


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe 
                165                 170                 175     


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
            180                 185                 190         


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu 
        195                 200                 205             


Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
    210                 215                 220                 


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys 
225                 230                 235                 240 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
                245                 250                 255     


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
            260                 265                 270         


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
        275                 280                 285             


Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser 
    290                 295                 300                 


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
305                 310                 315                 320 


Leu Ser Leu Ser Leu Gly 
                325     


<210>  42
<211>  326
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(326)
<223>  Human IgG4 double mutant (S228P) (L235A) (terminal K absent)

<400>  42

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro 
            100                 105                 110         


Glu Phe Ala Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
        115                 120                 125             


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
    130                 135                 140                 


Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp 
145                 150                 155                 160 


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe 
                165                 170                 175     


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
            180                 185                 190         


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu 
        195                 200                 205             


Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
    210                 215                 220                 


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys 
225                 230                 235                 240 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
                245                 250                 255     


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
            260                 265                 270         


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
        275                 280                 285             


Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser 
    290                 295                 300                 


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
305                 310                 315                 320 


Leu Ser Leu Ser Leu Gly 
                325     


<210>  43
<211>  297
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: CD20 amino acid sequence

<400>  43

Met Thr Thr Pro Arg Asn Ser Val Asn Gly Thr Phe Pro Ala Glu Pro 
1               5                   10                  15      


Met Lys Gly Pro Ile Ala Met Gln Ser Gly Pro Lys Pro Leu Phe Arg 
            20                  25                  30          


Arg Met Ser Ser Leu Val Gly Pro Thr Gln Ser Phe Phe Met Arg Glu 
        35                  40                  45              


Ser Lys Thr Leu Gly Ala Val Gln Ile Met Asn Gly Leu Phe His Ile 
    50                  55                  60                  


Ala Leu Gly Gly Leu Leu Met Ile Pro Ala Gly Ile Tyr Ala Pro Ile 
65                  70                  75                  80  


Cys Val Thr Val Trp Tyr Pro Leu Trp Gly Gly Ile Met Tyr Ile Ile 
                85                  90                  95      


Ser Gly Ser Leu Leu Ala Ala Thr Glu Lys Asn Ser Arg Lys Cys Leu 
            100                 105                 110         


Val Lys Gly Lys Met Ile Met Asn Ser Leu Ser Leu Phe Ala Ala Ile 
        115                 120                 125             


Ser Gly Met Ile Leu Ser Ile Met Asp Ile Leu Asn Ile Lys Ile Ser 
    130                 135                 140                 


His Phe Leu Lys Met Glu Ser Leu Asn Phe Ile Arg Ala His Thr Pro 
145                 150                 155                 160 


Tyr Ile Asn Ile Tyr Asn Cys Glu Pro Ala Asn Pro Ser Glu Lys Asn 
                165                 170                 175     


Ser Pro Ser Thr Gln Tyr Cys Tyr Ser Ile Gln Ser Leu Phe Leu Gly 
            180                 185                 190         


Ile Leu Ser Val Met Leu Ile Phe Ala Phe Phe Gln Glu Leu Val Ile 
        195                 200                 205             


Ala Gly Ile Val Glu Asn Glu Trp Lys Arg Thr Cys Ser Arg Pro Lys 
    210                 215                 220                 


Ser Asn Ile Val Leu Leu Ser Ala Glu Glu Lys Lys Glu Gln Thr Ile 
225                 230                 235                 240 


Glu Ile Lys Glu Glu Val Val Gly Leu Thr Glu Thr Ser Ser Gln Pro 
                245                 250                 255     


Lys Asn Glu Glu Asp Ile Glu Ile Ile Pro Ile Gln Glu Glu Glu Glu 
            260                 265                 270         


Glu Glu Thr Glu Thr Asn Phe Pro Glu Pro Pro Gln Asp Gln Glu Ser 
        275                 280                 285             


Ser Pro Ile Glu Asn Asp Ser Ser Pro 
    290                 295         


<210>  44
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: FLAG

<400>  44

Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


<210>  45
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: polyhistidine (6-His)

<400>  45

His His His His His His 
1               5       


<210>  46
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: hemagglutinin (HA)

<400>  46

Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
1               5                   


<210>  47
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: HCDR1.1 (anti-CD3) amino acid sequence

<400>  47

Gly Tyr Thr Phe Thr Arg Tyr Thr Met His 
1               5                   10  


<210>  48
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: HCDR1.2 (anti-CD3) amino acid sequence

<400>  48

Arg Tyr Thr Met His 
1               5   


<210>  49
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: HCDR2 (anti-CD3) amino acid sequence

<400>  49

Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Phe Lys 
1               5                   10                  15      


Asp 
    


<210>  50
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: HCDR3 (anti-CD3) amino acid sequence

<400>  50

Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr 
1               5                   10  


<210>  51
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: LCDR1 (anti-CD3) amino acid sequence

<400>  51

Arg Ala Ser Ser Ser Val Ser Tyr Met Asn 
1               5                   10  


<210>  52
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: LCDR2 (anti-CD3) amino acid sequence

<400>  52

Asp Thr Ser Lys Val Ala Ser 
1               5           


<210>  53
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: LCDR3 (anti-CD3) amino acid sequence

<400>  53

Gln Gln Trp Ser Ser Asn Pro Leu Thr 
1               5                   


<210>  54
<211>  294
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(294)
<223>  Human wild type nucleophosmin (amino acid sequence)

<400>  54

Met Glu Asp Ser Met Asp Met Asp Met Ser Pro Leu Arg Pro Gln Asn 
1               5                   10                  15      


Tyr Leu Phe Gly Cys Glu Leu Lys Ala Asp Lys Asp Tyr His Phe Lys 
            20                  25                  30          


Val Asp Asn Asp Glu Asn Glu His Gln Leu Ser Leu Arg Thr Val Ser 
        35                  40                  45              


Leu Gly Ala Gly Ala Lys Asp Glu Leu His Ile Val Glu Ala Glu Ala 
    50                  55                  60                  


Met Asn Tyr Glu Gly Ser Pro Ile Lys Val Thr Leu Ala Thr Leu Lys 
65                  70                  75                  80  


Met Ser Val Gln Pro Thr Val Ser Leu Gly Gly Phe Glu Ile Thr Pro 
                85                  90                  95      


Pro Val Val Leu Arg Leu Lys Cys Gly Ser Gly Pro Val His Ile Ser 
            100                 105                 110         


Gly Gln His Leu Val Ala Val Glu Glu Asp Ala Glu Ser Glu Asp Glu 
        115                 120                 125             


Glu Glu Glu Asp Val Lys Leu Leu Ser Ile Ser Gly Lys Arg Ser Ala 
    130                 135                 140                 


Pro Gly Gly Gly Ser Lys Val Pro Gln Lys Lys Val Lys Leu Ala Ala 
145                 150                 155                 160 


Asp Glu Asp Asp Asp Asp Asp Asp Glu Glu Asp Asp Asp Glu Asp Asp 
                165                 170                 175     


Asp Asp Asp Asp Phe Asp Asp Glu Glu Ala Glu Glu Lys Ala Pro Val 
            180                 185                 190         


Lys Lys Ser Ile Arg Asp Thr Pro Ala Lys Asn Ala Gln Lys Ser Asn 
        195                 200                 205             


Gln Asn Gly Lys Asp Ser Lys Pro Ser Ser Thr Pro Arg Ser Lys Gly 
    210                 215                 220                 


Gln Glu Ser Phe Lys Lys Gln Glu Lys Thr Pro Lys Thr Pro Lys Gly 
225                 230                 235                 240 


Pro Ser Ser Val Glu Asp Ile Lys Ala Lys Met Gln Ala Ser Ile Glu 
                245                 250                 255     


Lys Gly Gly Ser Leu Pro Lys Val Glu Ala Lys Phe Ile Asn Tyr Val 
            260                 265                 270         


Lys Asn Cys Phe Arg Met Thr Asp Gln Glu Ala Ile Gln Asp Leu Trp 
        275                 280                 285             


Gln Trp Arg Lys Ser Leu 
    290                 


<210>  55
<211>  17
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(17)
<223>  C-terminus of human wild type nucleophosmin  (amino acid 
       sequence)

<400>  55

Met Thr Asp Gln Glu Ala Ile Gln Asp Leu Trp Gln Trp Arg Lys Ser 
1               5                   10                  15      


Leu 
    


<210>  56
<211>  298
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(298)
<223>  Human nucleophosmin encoded by mutant NPM1c gene (amino acid 
       sequence)

<400>  56

Met Glu Asp Ser Met Asp Met Asp Met Ser Pro Leu Arg Pro Gln Asn 
1               5                   10                  15      


Tyr Leu Phe Gly Cys Glu Leu Lys Ala Asp Lys Asp Tyr His Phe Lys 
            20                  25                  30          


Val Asp Asn Asp Glu Asn Glu His Gln Leu Ser Leu Arg Thr Val Ser 
        35                  40                  45              


Leu Gly Ala Gly Ala Lys Asp Glu Leu His Ile Val Glu Ala Glu Ala 
    50                  55                  60                  


Met Asn Tyr Glu Gly Ser Pro Ile Lys Val Thr Leu Ala Thr Leu Lys 
65                  70                  75                  80  


Met Ser Val Gln Pro Thr Val Ser Leu Gly Gly Phe Glu Ile Thr Pro 
                85                  90                  95      


Pro Val Val Leu Arg Leu Lys Cys Gly Ser Gly Pro Val His Ile Ser 
            100                 105                 110         


Gly Gln His Leu Val Ala Val Glu Glu Asp Ala Glu Ser Glu Asp Glu 
        115                 120                 125             


Glu Glu Glu Asp Val Lys Leu Leu Ser Ile Ser Gly Lys Arg Ser Ala 
    130                 135                 140                 


Pro Gly Gly Gly Ser Lys Val Pro Gln Lys Lys Val Lys Leu Ala Ala 
145                 150                 155                 160 


Asp Glu Asp Asp Asp Asp Asp Asp Glu Glu Asp Asp Asp Glu Asp Asp 
                165                 170                 175     


Asp Asp Asp Asp Phe Asp Asp Glu Glu Ala Glu Glu Lys Ala Pro Val 
            180                 185                 190         


Lys Lys Ser Ile Arg Asp Thr Pro Ala Lys Asn Ala Gln Lys Ser Asn 
        195                 200                 205             


Gln Asn Gly Lys Asp Ser Lys Pro Ser Ser Thr Pro Arg Ser Lys Gly 
    210                 215                 220                 


Gln Glu Ser Phe Lys Lys Gln Glu Lys Thr Pro Lys Thr Pro Lys Gly 
225                 230                 235                 240 


Pro Ser Ser Val Glu Asp Ile Lys Ala Lys Met Gln Ala Ser Ile Glu 
                245                 250                 255     


Lys Gly Gly Ser Leu Pro Lys Val Glu Ala Lys Phe Ile Asn Tyr Val 
            260                 265                 270         


Lys Asn Cys Phe Arg Met Thr Asp Gln Glu Ala Ile Gln Asp Leu Cys 
        275                 280                 285             


Leu Ala Val Glu Glu Val Ser Leu Arg Lys 
    290                 295             


<210>  57
<211>  21
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(21)
<223>  C-terminus of human nucleophosmin encoded by mutant NPM1c gene 
       (amino acid sequence)

<400>  57

Met Thr Asp Gln Glu Ala Ile Gln Asp Leu Cys Leu Ala Val Glu Glu 
1               5                   10                  15      


Val Ser Leu Arg Lys 
            20      


<210>  58
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Linker

<400>  58

Gly Gly Gly Gly Ser 
1               5   


<210>  59
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Linker

<400>  59

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10  


<210>  60
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Linker

<400>  60

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  61
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Linker

<400>  61

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser 
            20  


<210>  62
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: NY-ESO-1 neoepitope (SLL)

<400>  62

Ser Leu Leu Met Trp Ile Thr Gln Cys 
1               5                   


<210>  63
<211>  9
<212>  PRT
<213>  Influenza virus


<220>
<221>  misc_feature
<222>  (1)..(9)
<223>  influenza virus M1 protein neoepitope (GIL)

<400>  63

Gly Ile Leu Gly Phe Val Phe Thr Leu 
1               5                   


<210>  64
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Forward primer

<400>  64
gtcagtaatt gcggttctca cc                                                22


<210>  65
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Reverse primer

<400>  65
gtacagtggg aacaaagtcg                                                   20


<210>  66
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Forward primer

<400>  66
ccggggtaga acctaaaagt tccg                                              24


<210>  67
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Reverse primer

<400>  67
tttgttctgc acgcgtggat c                                                 21


<210>  68
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Forward primer

<400>  68
gggtaattaa tcagcgaagc gatg                                              24


<210>  69
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Forward primer

<400>  69
gttaggccag cttggcactt gatgt                                             25


<210>  70
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Reverse primer

<400>  70
aggcacaatc agcattggta gctg                                              24


<210>  71
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: NPM1c neoepitope

<400>  71

Ala Ile Gln Asp Leu Cys Val Ala Val 
1               5                   


<210>  72
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: NPM1c neoepitope

<400>  72

Cys Leu Ala Val Glu Glu Val Ser Leu 
1               5                   


<210>  73
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: NPM1c neoepitope

<400>  73

Val Glu Glu Val Ser Leu Arg Lys 
1               5               


<210>  74
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: NPM1c neoepitope

<400>  74

Ala Val Glu Glu Val Ser Leu Arg 
1               5               


<210>  75
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: NPM1c neoepitope

<400>  75

Ala Val Glu Glu Val Ser Leu Arg Lys 
1               5                   


<210>  76
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: NPM1c neoepitope

<400>  76

Cys Leu Ala Val Glu Glu Val Ser Leu Arg Lys 
1               5                   10      


<210>  77
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: AIQ X1 substitution

<400>  77

Val Ile Gln Asp Leu Cys Leu Ala Val 
1               5                   


<210>  78
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: AIQ X1 substitution

<400>  78

Leu Ile Gln Asp Leu Cys Leu Ala Val 
1               5                   


<210>  79
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: AIQ X1 substitution

<400>  79

Ile Ile Gln Asp Leu Cys Leu Ala Val 
1               5                   


<210>  80
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: AIQ X3 substitution

<400>  80

Ala Ile Asn Asp Leu Cys Leu Ala Val 
1               5                   


<210>  81
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: AIQ X4 substitution

<400>  81

Ala Ile Gln Glu Leu Cys Leu Ala Val 
1               5                   


<210>  82
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: AIQ X5 substitution

<400>  82

Ala Ile Gln Asp Ile Cys Leu Ala Val 
1               5                   


<210>  83
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: AIQ X5 substitution

<400>  83

Ala Ile Gln Asp Val Cys Leu Ala Val 
1               5                   


<210>  84
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: AIQ X5 substitution

<400>  84

Ala Ile Gln Asp Met Cys Leu Ala Val 
1               5                   


<210>  85
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: AIQ X5 substitution

<400>  85

Ala Ile Gln Asp Ala Cys Leu Ala Val 
1               5                   


<210>  86
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: AIQ X5 substitution

<400>  86

Ala Ile Gln Asp Phe Cys Leu Ala Val 
1               5                   


<210>  87
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: AIQ X6 substitution

<400>  87

Ala Ile Gln Asp Leu Ser Leu Ala Val 
1               5                   


<210>  88
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: AIQ X6 substitution

<400>  88

Ala Ile Gln Asp Leu Ala Leu Ala Val 
1               5                   


<210>  89
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: AIQ X7 substitution

<400>  89

Ala Ile Gln Asp Leu Cys Ile Ala Val 
1               5                   


<210>  90
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: AIQ X7 substitution

<400>  90

Ala Ile Gln Asp Leu Cys Val Ala Val 
1               5                   


<210>  91
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: AIQ X7 substitution

<400>  91

Ala Ile Gln Asp Leu Cys Met Ala Val 
1               5                   


<210>  92
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: AIQ X7 substitution

<400>  92

Ala Ile Gln Asp Leu Cys Ala Ala Val 
1               5                   


<210>  93
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: AIQ X7 substitution

<400>  93

Ala Ile Gln Asp Leu Cys Phe Ala Val 
1               5                   


<210>  94
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: AIQ X8 substitution

<400>  94

Ala Ile Gln Asp Leu Cys Leu Val Val 
1               5                   


<210>  95
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: AIQ X8 substitution

<400>  95

Ala Ile Gln Asp Leu Cys Leu Leu Val 
1               5                   


<210>  96
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: AIQ X8 substitution

<400>  96

Ala Ile Gln Asp Leu Cys Leu Ile Val 
1               5                   


