                         SEQUENCE LISTING

<110>  iNtRON Biotechnology, Inc.
 
<120>  ANTIBACTERIAL PROTEIN HAVING LYTIC ACTIVITY TO BACILLUS GENUS AND
        METHOD FOR PREPARING THE SAME

<130>  200010053WO

<150>  US 62/986,904
<151>  2020-03-09

<160>  2     

<170>  PatentIn version 3.5

<210>  1
<211>  247
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Antibacterial protein BAL200

<400>  1

Met Ala Met Ala Leu Gln Thr Leu Ile Asp Lys Ala Asn Arg Lys Leu 
1               5                   10                  15      


Asn Val Ser Gly Met Arg Lys Asp Val Ala Asp Arg Thr Arg Ala Val 
            20                  25                  30          


Ile Thr Gln Met His Ala Ser Gly Ile Tyr Ile Cys Val Ala Gln Gly 
        35                  40                  45              


Trp Arg Ser Phe Ala Glu Gln Asn Ala Leu Tyr Ala Gln Gly Arg Thr 
    50                  55                  60                  


Lys Pro Gly Ser Ile Val Thr Asn Ala Arg Gly Gly Gln Ser Asn His 
65                  70                  75                  80  


Asn Tyr Gly Val Ala Val Asp Leu Cys Leu Tyr Thr Gln Asp Gly Ser 
                85                  90                  95      


Asp Val Ile Trp Thr Val Glu Gly Asn Phe Arg Lys Val Ile Ala Ala 
            100                 105                 110         


Met Lys Ala Gln Gly Phe Lys Trp Gly Gly Asp Trp Val Ser Phe Lys 
        115                 120                 125             


Asp Tyr Pro His Phe Glu Leu Tyr Asp Val Val Gly Gly Gly Ser Gly 
    130                 135                 140                 


Ser Thr Gly Gly Ser Gly Gly Gly Ser Thr Gly Gly Gly Ser Thr Gly 
145                 150                 155                 160 


Gly Leu Tyr Asp Ser Ser Trp Phe Thr Lys Glu Thr Gly Thr Phe Val 
                165                 170                 175     


Thr Asn Thr Ser Ile Lys Leu Arg Thr Ala Pro Phe Thr Ser Ala Asp 
            180                 185                 190         


Val Ile Ala Thr Leu Pro Ala Gly Ser Pro Val Asn Tyr Asn Gly Phe 
        195                 200                 205             


Gly Ile Glu Tyr Asp Gly Tyr Val Trp Ile Arg Gln Pro Arg Ser Asn 
    210                 215                 220                 


Gly Tyr Gly Tyr Leu Ala Thr Gly Glu Ser Lys Gly Gly Lys Arg Gln 
225                 230                 235                 240 


Asn Tyr Trp Gly Thr Phe Lys 
                245         


<210>  2
<211>  4811
<212>  DNA
<213>  Artificial sequence

<220>
<223>  BL21-pBAD-BAL200 cell plasmid

<400>  2
aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct       60

tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca      120

aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg      180

attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg      240

atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg      300

ctagaaataa ttttgtttaa ctttaagaag gagatataca tccatggcaa tggcattaca      360

aactttaatc gacaaggcga accgtaaatt gaacgtttct ggtatgcgta aggacgtagc      420

agaccgtacc cgcgctgtca ttacacaaat gcatgcatcc ggtatttata tctgtgtagc      480

acaaggttgg cgttcgtttg ctgaacagaa cgctttatac gcgcaaggtc gtactaaacc      540

gggtagcatc gtaacaaatg cacgaggcgg acaatcgaac cacaactacg gagtagcggt      600

agacttatgc ttgtacacac aagacggttc tgacgttatc tggacagttg aaggtaattt      660

ccgtaaggtt atcgcagcaa tgaaagcaca aggcttcaaa tggggcggag attgggtttc      720

atttaaagat taccctcact ttgaattgta cgatgtagta ggcggaggct ctggtagcac      780

aggcggttct ggcggaggaa gtacaggagg tggctctaca ggtggactgt acgattctag      840

ctggtttaca aaagagactg gtactttcgt aacaaatact tcaatcaaat tacgtacagc      900

accattcaca agtgcagacg taatcgctac acttccggct ggttctccag ttaactacaa      960

tggcttcggt atcgaatatg atggttacgt ttggattcgt caaccacgta gcaatggtta     1020

cggctatctt gctacaggtg aatctaaagg cggaaaacgt cagaactact ggggtacgtt     1080

caaataagcg gccgcaaggg cgagcttgaa ggtaagccta tccctaaccc tctcctcggt     1140

ctcgattcta cgcgtaccgg tcatcatcac catcaccatt gagtttaaac ggtctccagc     1200

ttggctgttt tggcggatga gagaagattt tcagcctgat acagattaaa tcagaacgca     1260

gaagcggtct gataaaacag aatttgcctg gcggcagtag cgcggtggtc ccacctgacc     1320

ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg tctccccatg     1380

cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc     1440

tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga     1500

gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa     1560

actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt tgcgtttcta     1620

caaactcttt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagattatc     1680

aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag     1740

tatatatgag taaacttggt ctgacagtta ggcgtcgctt ggtcggtcat ttcgaacccc     1800

agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg     1860

gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag     1920

caatatcacg ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac     1980

agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc     2040

catgtgtcac gacgagatcc tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt     2100

cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt     2160

ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag     2220

ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag     2280

gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc     2340

ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc     2400

acgatagccg cgctgcctcg tcctgcagtt cattcagggc accggacagg tcggtcttga     2460

caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga     2520

ttgtcagttg tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg     2580

cgtgcaatcc atcttgttca atcatactct tcctttttca atattattga agcatttatc     2640

agggttattg tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag     2700

accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct     2760

gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac     2820

caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc     2880

tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg     2940

ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt     3000

tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt     3060

gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc     3120

tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca     3180

gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata     3240

gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg     3300

ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gcattttgct     3360

ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta     3420

ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag     3480

tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta     3540

tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc     3600

agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac     3660

acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt     3720

gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag     3780

gcagcagatc aattcgcgcg cgaaggcgaa gcggcatgca taatgtgcct gtcaaatgga     3840

cgaagcaggg attctgcaaa ccctatgcta ctccgtcaag ccgtcaattg tctgattcgt     3900

taccaattat gacaacttga cggctacatc attcactttt tcttcacaac cggcacggaa     3960

ctcgctcggg ctggccccgg tgcatttttt aaatacccgc gagaaataga gttgatcgtc     4020

aaaaccaaca ttgcgaccga cggtggcgat aggcatccgg gtggtgctca aaagcagctt     4080

cgcctggctg atacgttggt cctcgcgcca gcttaagacg ctaatcccta actgctggcg     4140

gaaaagatgt gacagacgcg acggcgacaa gcaaacatgc tgtgcgacgc tggcgatatc     4200

aaaattgctg tctgccaggt gatcgctgat gtactgacaa gcctcgcgta cccgattatc     4260

catcggtgga tggagcgact cgttaatcgc ttccatgcgc cgcagtaaca attgctcaag     4320

cagatttatc gccagcagct ccgaatagcg cccttcccct tgcccggcgt taatgatttg     4380

cccaaacagg tcgctgaaat gcggctggtg cgcttcatcc gggcgaaaga accccgtatt     4440

ggcaaatatt gacggccagt taagccattc atgccagtag gcgcgcggac gaaagtaaac     4500

ccactggtga taccattcgc gagcctccgg atgacgaccg tagtgatgaa tctctcctgg     4560

cgggaacagc aaaatatcac ccggtcggca aacaaattct cgtccctgat ttttcaccac     4620

cccctgaccg cgaatggtga gattgagaat ataacctttc attcccagcg gtcggtcgat     4680

aaaaaaatcg agataaccgt tggcctcaat cggcgttaaa cccgccacca gatgggcatt     4740

aaacgagtat cccggcagca ggggatcatt ttgcgcttca gccatacttt tcatactccc     4800

gccattcaga g                                                          4811


