                         SEQUENCE LISTING

<110>  University of Dundee
 
<120>  IL-10 Muteins

<130>  PE960351WO

<160>  24    

<170>  PatentIn version 3.5

<210>  1
<211>  178
<212>  PRT
<213>  Wild type dimeric IL-10

<400>  1

Met His Ser Ser Ala Leu Leu Cys Cys Leu Val Leu Leu Thr Gly Val 
1               5                   10                  15      


Arg Ala Ser Pro Gly Gln Gly Thr Gln Ser Glu Asn Ser Cys Thr His 
            20                  25                  30          


Phe Pro Gly Asn Leu Pro Asn Met Leu Arg Asp Leu Arg Asp Ala Phe 
        35                  40                  45              


Ser Arg Val Lys Thr Phe Phe Gln Met Lys Asp Gln Leu Asp Asn Leu 
    50                  55                  60                  


Leu Leu Lys Glu Ser Leu Leu Glu Asp Phe Lys Gly Tyr Leu Gly Cys 
65                  70                  75                  80  


Gln Ala Leu Ser Glu Met Ile Gln Phe Tyr Leu Glu Glu Val Met Pro 
                85                  90                  95      


Gln Ala Glu Asn Gln Asp Pro Asp Ile Lys Ala His Val Asn Ser Leu 
            100                 105                 110         


Gly Glu Asn Leu Lys Thr Leu Arg Leu Arg Leu Arg Arg Cys His Arg 
        115                 120                 125             


Phe Leu Pro Cys Glu Asn Lys Ser Lys Ala Val Glu Gln Val Lys Asn 
    130                 135                 140                 


Ala Phe Asn Lys Leu Gln Glu Lys Gly Ile Tyr Lys Ala Met Ser Glu 
145                 150                 155                 160 


Phe Asp Ile Phe Ile Asn Tyr Ile Glu Ala Tyr Met Thr Met Lys Ile 
                165                 170                 175     


Arg Asn 
        


<210>  2
<211>  534
<212>  DNA
<213>  Wild type dimeric IL-10

<400>  2
atgcacagct cagcactgct ctgttgcctg gtcctcctga ctggggtgag ggccagccca       60

ggccagggca cccagtccga gaacagttgc acgcactttc cgggcaacct tcctaacatg      120

cttcgcgacc tgcgagatgc gttctctcga gtgaaaactt tctttcagat gaaagatcag      180

ttggataatt tgctcctcaa ggagtccctt ctcgaagact ttaaaggata cctggggtgc      240

caggcgctta gcgagatgat acagttctat ctggaggaag tgatgcccca agcagaaaat      300

caagatcccg atattaaggc ccatgtcaac agccttggcg aaaatttgaa aactctcaga      360

ctccgacttc gccgatgcca caggtttttg ccatgtgaga ataagtccaa ggccgttgag      420

caggtgaaaa acgctttcaa caaactgcaa gaaaagggga tctacaaagc gatgtcagag      480

ttcgacatat ttataaacta catagaagcc tatatgacga tgaaaatccg caac            534


<210>  3
<211>  184
<212>  PRT
<213>  Wild type monomeric IL-10

<400>  3

Met His Ser Ser Ala Leu Leu Cys Cys Leu Val Leu Leu Thr Gly Val 
1               5                   10                  15      


Arg Ala Ser Pro Gly Gln Gly Thr Gln Ser Glu Asn Ser Cys Thr His 
            20                  25                  30          


Phe Pro Gly Asn Leu Pro Asn Met Leu Arg Asp Leu Arg Asp Ala Phe 
        35                  40                  45              


Ser Arg Val Lys Thr Phe Phe Gln Met Lys Asp Gln Leu Asp Asn Leu 
    50                  55                  60                  


Leu Leu Lys Glu Ser Leu Leu Glu Asp Phe Lys Gly Tyr Leu Gly Cys 
65                  70                  75                  80  


Gln Ala Leu Ser Glu Met Ile Gln Phe Tyr Leu Glu Glu Val Met Pro 
                85                  90                  95      


Gln Ala Glu Asn Gln Asp Pro Asp Ile Lys Ala His Val Asn Ser Leu 
            100                 105                 110         


Gly Glu Asn Leu Lys Thr Leu Arg Leu Arg Leu Arg Arg Cys His Arg 
        115                 120                 125             


Phe Leu Pro Cys Glu Asn Gly Gly Gly Ser Gly Gly Lys Ser Lys Ala 
    130                 135                 140                 


Val Glu Gln Val Lys Asn Ala Phe Asn Lys Leu Gln Glu Lys Gly Ile 
145                 150                 155                 160 


Tyr Lys Ala Met Ser Glu Phe Asp Ile Phe Ile Asn Tyr Ile Glu Ala 
                165                 170                 175     


Tyr Met Thr Met Lys Ile Arg Asn 
            180                 


<210>  4
<211>  552
<212>  DNA
<213>  Wild type monomeric IL-10

<400>  4
atgcacagct cagcactgct ctgttgcctg gtcctcctga ctggggtgag ggccagccca       60

ggccagggca cccagtccga gaacagttgc acgcactttc cgggcaacct tcctaacatg      120

cttcgcgacc tgcgagatgc gttctctcga gtgaaaactt tctttcagat gaaagatcag      180

ttggataatt tgctcctcaa ggagtccctt ctcgaagact ttaaaggata cctggggtgc      240

caggcgctta gcgagatgat acagttctat ctggaggaag tgatgcccca agcagaaaat      300

caagatcccg atattaaggc ccatgtcaac agccttggcg aaaatttgaa aactctcaga      360

ctccgacttc gccgatgcca caggtttttg ccatgtgaga atgggggtgg ttccgggggt      420

aagtccaagg ccgttgagca ggtgaaaaac gctttcaaca aactgcaaga aaaggggatc      480

tacaaagcga tgtcagagtt cgacatattt ataaactaca tagaagccta tatgacgatg      540

aaaatccgca ac                                                          552


<210>  5
<211>  178
<212>  PRT
<213>  A11 dimer mutein

<400>  5

Met His Ser Ser Ala Leu Leu Cys Cys Leu Val Leu Leu Thr Gly Val 
1               5                   10                  15      


Arg Ala Ser Pro Gly Gln Gly Thr Gln Ser Glu Asn Ser Cys Thr His 
            20                  25                  30          


Phe Pro Gly Tyr Leu Pro Asn Met Leu Arg Asp Leu Arg Asp Ala Phe 
        35                  40                  45              


Ser Arg Val Lys Thr Phe Phe Gln Met Lys Asp Gln Leu Asp Asn Leu 
    50                  55                  60                  


Leu Leu Lys Glu Ser Leu Leu Glu Asp Phe Lys Gly Tyr Leu Gly Cys 
65                  70                  75                  80  


Gln Ala Leu Ser Glu Met Ile Gln Phe Tyr Leu Glu Glu Val Met Pro 
                85                  90                  95      


Gln Ala Glu Asn Gln Asp Pro Asp Ile Lys Ala His Val Ile Ser Leu 
            100                 105                 110         


Gly Glu Asn Leu Asn Thr Leu Arg Leu Arg Leu Arg Arg Cys His Arg 
        115                 120                 125             


Phe Leu Pro Cys Glu Asn Lys Ser Lys Ala Val Glu Gln Val Lys Asn 
    130                 135                 140                 


Ala Phe Asn Lys Leu Gln Glu Lys Gly Ile Tyr Lys Ala Met Ser Glu 
145                 150                 155                 160 


Phe Asp Ile Phe Ile His Tyr Ile Glu Ala Tyr Met Thr Met Lys Ile 
                165                 170                 175     


Arg Asn 
        


<210>  6
<211>  534
<212>  DNA
<213>  A11 dimer mutein

<400>  6
atgcacagct cagcactgct ctgttgcctg gtcctcctga ctggggtgag ggccagccca       60

ggccagggca cccagtccga gaacagttgc acgcactttc cgggctacct tcctaacatg      120

cttcgcgacc tgcgagatgc gttctctcga gtgaaaactt tctttcagat gaaagatcag      180

ttggataatt tgctcctcaa ggagtccctt ctcgaagact ttaaaggata cctggggtgc      240

caggcgctta gcgagatgat acagttctat ctggaggaag tgatgcccca agcagaaaat      300

caagatcccg atattaaggc ccatgtcatc agccttggcg aaaatttgaa cactctcaga      360

ctccgacttc gccgatgcca caggtttttg ccatgtgaga ataagtccaa ggccgttgag      420

caggtgaaaa acgctttcaa caaactgcaa gaaaagggga tctacaaagc gatgtcagag      480

ttcgacatat ttatacacta catagaagcc tatatgacga tgaaaatccg caac            534


<210>  7
<211>  178
<212>  PRT
<213>  B11 dimer mutein

<400>  7

Met His Ser Ser Ala Leu Leu Cys Cys Leu Val Leu Leu Thr Gly Val 
1               5                   10                  15      


Arg Ala Ser Pro Gly Gln Gly Thr Gln Ser Glu Asn Ser Cys Thr His 
            20                  25                  30          


Phe Pro Gly Tyr Leu Pro Asn Met Leu Arg Asp Leu Arg Asp Ala Phe 
        35                  40                  45              


Ser Arg Val Lys Thr Phe Phe Gln Met Lys Asp Gln Leu Asp Asn Leu 
    50                  55                  60                  


Leu Leu Lys Glu Ser Leu Leu Glu Val Phe Lys Gly Tyr Leu Gly Cys 
65                  70                  75                  80  


Gln Ala Leu Ser Glu Met Met Gln Phe Tyr Leu Glu Glu Val Met Pro 
                85                  90                  95      


Gln Ala Glu Asn Gln Asp Pro Asp Ile Lys Ala His Val Ile Ser Leu 
            100                 105                 110         


Gly Glu Lys Leu Lys Thr Leu Arg Leu Arg Leu Arg Arg Cys His Trp 
        115                 120                 125             


Phe Leu Pro Cys Glu Asn Lys Ser Lys Ala Val Glu Gln Val Lys Asn 
    130                 135                 140                 


Ala Phe Asn Lys Leu Gln Glu Lys Gly Ile Tyr Lys Ala Met Ser Glu 
145                 150                 155                 160 


Phe Asp Ile Phe Ile Asn Tyr Ile Glu Ala Tyr Met Thr Met Lys Ile 
                165                 170                 175     


Arg Asn 
        


<210>  8
<211>  534
<212>  DNA
<213>  B11 dimer mutein

<400>  8
atgcacagct cagcactgct ctgttgcctg gtcctcctga ctggggtgag ggccagccca       60

ggccagggca cccagtccga gaacagttgc acgcactttc cgggctacct tcctaacatg      120

cttcgcgacc tgcgagatgc gttctctcga gtgaaaactt tctttcagat gaaagatcag      180

ttggataatt tgctcctcaa ggagtccctt ctcgaagtct ttaaaggata cctggggtgc      240

caggcgctta gcgagatgat gcagttctat ctggaggaag tgatgcccca agcagaaaat      300

caagatcccg atattaaggc ccatgtcatc agccttggcg aaaagttgaa aactctcaga      360

ctccgacttc gccgatgcca ctggtttttg ccatgtgaga ataagtccaa ggccgttgag      420

caggtgaaaa acgctttcaa caaactgcaa gaaaagggga tctacaaagc gatgtcagag      480

ttcgacatat ttataaacta catagaagcc tatatgacga tgaaaatccg caac            534


<210>  9
<211>  184
<212>  PRT
<213>  R5A11 monomer mutein

<400>  9

Met His Ser Ser Ala Leu Leu Cys Cys Leu Val Leu Leu Thr Gly Val 
1               5                   10                  15      


Arg Ala Ser Pro Gly Gln Gly Thr Gln Ser Glu Asn Ser Cys Thr His 
            20                  25                  30          


Phe Pro Gly Ile Leu Pro Asn Met Leu Arg Asp Leu Arg Asp Ala Phe 
        35                  40                  45              


Ser Arg Val Lys Thr Phe Phe Gln Met Lys Asp Gln Leu Asp Asn Leu 
    50                  55                  60                  


Leu Leu Lys Glu Ser Leu Leu Glu Asp Phe Lys Gly Tyr Leu Gly Cys 
65                  70                  75                  80  


Gln Ala Leu Ser Glu Met Ile Gln Phe Tyr Leu Glu Glu Val Met Pro 
                85                  90                  95      


Gln Ala Glu Asn Gln Asp Pro Asp Ile Lys Ala His Val Ile Ser Leu 
            100                 105                 110         


Gly Glu Asn Leu Asn Thr Leu Arg Leu Arg Leu Arg Arg Cys His Arg 
        115                 120                 125             


Leu Leu Pro Cys Glu Asn Gly Gly Gly Ser Gly Gly Lys Ser Lys Ala 
    130                 135                 140                 


Val Glu Gln Val Lys Asn Ala Phe Asn Lys Leu Gln Glu Lys Gly Ile 
145                 150                 155                 160 


Tyr Lys Ala Met Ser Glu Phe Asp Ile Phe Ile Asn Tyr Ile Glu Ala 
                165                 170                 175     


Tyr Met Thr Met Lys Ile Arg Asn 
            180                 


<210>  10
<211>  552
<212>  DNA
<213>  R5A11 monomer mutein

<400>  10
atgcacagct cagcactgct ctgttgcctg gtcctcctga ctggggtgag ggccagccca       60

ggccagggca cccagtccga gaacagttgc acgcactttc cgggcatcct tcctaacatg      120

cttcgcgacc tgcgagatgc gttctctcga gtgaaaactt tctttcagat gaaagatcag      180

ttggataatt tgctcctcaa ggagtccctt ctcgaagact ttaaaggata cctggggtgc      240

caggcgctta gcgagatgat acagttctat ctggaggaag tgatgcccca agcagaaaat      300

caagatcccg atattaaggc ccatgtcatc agccttggcg aaaatttgaa tactctcaga      360

ctccgacttc gccgatgcca caggcttttg ccatgtgaga atgggggtgg ttccgggggt      420

aagtccaagg ccgttgagca ggtgaaaaac gctttcaaca aactgcaaga aaaggggatc      480

tacaaagcga tgtcagagtt cgacatattt ataaactaca tagaagccta tatgacgatg      540

aaaatccgca ac                                                          552


<210>  11
<211>  178
<212>  PRT
<213>  R5A11 dimer mutein

<400>  11

Met His Ser Ser Ala Leu Leu Cys Cys Leu Val Leu Leu Thr Gly Val 
1               5                   10                  15      


Arg Ala Ser Pro Gly Gln Gly Thr Gln Ser Glu Asn Ser Cys Thr His 
            20                  25                  30          


Phe Pro Gly Ile Leu Pro Asn Met Leu Arg Asp Leu Arg Asp Ala Phe 
        35                  40                  45              


Ser Arg Val Lys Thr Phe Phe Gln Met Lys Asp Gln Leu Asp Asn Leu 
    50                  55                  60                  


Leu Leu Lys Glu Ser Leu Leu Glu Asp Phe Lys Gly Tyr Leu Gly Cys 
65                  70                  75                  80  


Gln Ala Leu Ser Glu Met Ile Gln Phe Tyr Leu Glu Glu Val Met Pro 
                85                  90                  95      


Gln Ala Glu Asn Gln Asp Pro Asp Ile Lys Ala His Val Ile Ser Leu 
            100                 105                 110         


Gly Glu Asn Leu Asn Thr Leu Arg Leu Arg Leu Arg Arg Cys His Arg 
        115                 120                 125             


Leu Leu Pro Cys Glu Asn Lys Ser Lys Ala Val Glu Gln Val Lys Asn 
    130                 135                 140                 


Ala Phe Asn Lys Leu Gln Glu Lys Gly Ile Tyr Lys Ala Met Ser Glu 
145                 150                 155                 160 


Phe Asp Ile Phe Ile Asn Tyr Ile Glu Ala Tyr Met Thr Met Lys Ile 
                165                 170                 175     


Arg Asn 
        


<210>  12
<211>  534
<212>  DNA
<213>  R5A11 dimer mutein

<400>  12
atgcacagct cagcactgct ctgttgcctg gtcctcctga ctggggtgag ggccagccca       60

ggccagggca cccagtccga gaacagttgc acgcactttc cgggcatcct tcctaacatg      120

cttcgcgacc tgcgagatgc gttctctcga gtgaaaactt tctttcagat gaaagatcag      180

ttggataatt tgctcctcaa ggagtccctt ctcgaagact ttaaaggata cctggggtgc      240

caggcgctta gcgagatgat acagttctat ctggaggaag tgatgcccca agcagaaaat      300

caagatcccg atattaaggc ccatgtcatc agccttggcg aaaatttgaa tactctcaga      360

ctccgacttc gccgatgcca caggcttttg ccatgtgaga ataagtccaa ggccgttgag      420

caggtgaaaa acgctttcaa caaactgcaa gaaaagggga tctacaaagc gatgtcagag      480

ttcgacatat ttataaacta catagaagcc tatatgacga tgaaaatccg caac            534


<210>  13
<211>  184
<212>  PRT
<213>  G3 monomer mutein

<400>  13

Met His Ser Ser Ala Leu Leu Cys Cys Leu Val Leu Leu Thr Gly Val 
1               5                   10                  15      


Arg Ala Ser Pro Gly Gln Gly Thr Gln Ser Glu Asn Ser Cys Thr His 
            20                  25                  30          


Phe Pro Gly Tyr Leu Pro Asn Met Leu Arg Asp Leu Arg Asp Ala Phe 
        35                  40                  45              


Ser Arg Val Lys Thr Phe Phe Gln Met Lys Asp Gln Leu Asp Asn Leu 
    50                  55                  60                  


Leu Leu Lys Glu Ser Leu Leu Glu Val Phe Lys Gly Tyr Leu Gly Cys 
65                  70                  75                  80  


Gln Ala Leu Ser Glu Met Met Gln Phe Tyr Leu Glu Glu Val Met Pro 
                85                  90                  95      


Gln Ala Glu Asn Gln Asp Pro Asp Ile Lys Ala His Val Ile Ser Leu 
            100                 105                 110         


Gly Glu Asn Leu Asn Thr Leu Arg Leu Arg Leu Arg Arg Cys His Trp 
        115                 120                 125             


Phe Leu Pro Cys Glu Asn Gly Gly Gly Ser Gly Gly Lys Ser Lys Ala 
    130                 135                 140                 


Val Glu Gln Val Lys Asn Ala Phe Asn Lys Leu Gln Glu Lys Gly Ile 
145                 150                 155                 160 


Tyr Lys Ala Met Ser Glu Phe Asp Ile Phe Ile Asn Tyr Ile Glu Ala 
                165                 170                 175     


Tyr Met Thr Met Lys Ile Arg Asn 
            180                 


<210>  14
<211>  552
<212>  DNA
<213>  G3 monomer mutein

<400>  14
atgcacagct cagcactgct ctgttgcctg gtcctcctga ctggggtgag ggccagccca       60

ggccagggca cccagtccga gaacagttgc acgcactttc cgggctacct tcctaacatg      120

cttcgcgacc tgcgagatgc gttctctcga gtgaaaactt tctttcagat gaaagatcag      180

ttggataatt tgctcctcaa ggagtccctt ctcgaagtct ttaaaggata cctggggtgc      240

caggcgctta gcgagatgat gcagttctat ctggaggaag tgatgcccca agcagaaaat      300

caagatcccg atattaaggc ccatgtcatc agccttggcg aaaatttgaa cactctcaga      360

ctccgacttc gccgatgcca ctggtttttg ccatgtgaga atgggggtgg ttccgggggt      420

aagtccaagg ccgttgagca ggtgaaaaac gctttcaaca aactgcaaga aaaggggatc      480

tacaaagcga tgtcagagtt cgacatattt ataaactaca tagaagccta tatgacgatg      540

aaaatccgca ac                                                          552


<210>  15
<211>  178
<212>  PRT
<213>  G3 dimer mutein

<400>  15

Met His Ser Ser Ala Leu Leu Cys Cys Leu Val Leu Leu Thr Gly Val 
1               5                   10                  15      


Arg Ala Ser Pro Gly Gln Gly Thr Gln Ser Glu Asn Ser Cys Thr His 
            20                  25                  30          


Phe Pro Gly Tyr Leu Pro Asn Met Leu Arg Asp Leu Arg Asp Ala Phe 
        35                  40                  45              


Ser Arg Val Lys Thr Phe Phe Gln Met Lys Asp Gln Leu Asp Asn Leu 
    50                  55                  60                  


Leu Leu Lys Glu Ser Leu Leu Glu Val Phe Lys Gly Tyr Leu Gly Cys 
65                  70                  75                  80  


Gln Ala Leu Ser Glu Met Met Gln Phe Tyr Leu Glu Glu Val Met Pro 
                85                  90                  95      


Gln Ala Glu Asn Gln Asp Pro Asp Ile Lys Ala His Val Ile Ser Leu 
            100                 105                 110         


Gly Glu Asn Leu Asn Thr Leu Arg Leu Arg Leu Arg Arg Cys His Trp 
        115                 120                 125             


Phe Leu Pro Cys Glu Asn Lys Ser Lys Ala Val Glu Gln Val Lys Asn 
    130                 135                 140                 


Ala Phe Asn Lys Leu Gln Glu Lys Gly Ile Tyr Lys Ala Met Ser Glu 
145                 150                 155                 160 


Phe Asp Ile Phe Ile Asn Tyr Ile Glu Ala Tyr Met Thr Met Lys Ile 
                165                 170                 175     


Arg Asn 
        


<210>  16
<211>  534
<212>  DNA
<213>  G3 dimer mutein

<400>  16
atgcacagct cagcactgct ctgttgcctg gtcctcctga ctggggtgag ggccagccca       60

ggccagggca cccagtccga gaacagttgc acgcactttc cgggctacct tcctaacatg      120

cttcgcgacc tgcgagatgc gttctctcga gtgaaaactt tctttcagat gaaagatcag      180

ttggataatt tgctcctcaa ggagtccctt ctcgaagtct ttaaaggata cctggggtgc      240

caggcgctta gcgagatgat gcagttctat ctggaggaag tgatgcccca agcagaaaat      300

caagatcccg atattaaggc ccatgtcatc agccttggcg aaaatttgaa tactctcaga      360

ctccgacttc gccgatgcca ctggtttttg ccatgtgaga ataagtccaa ggccgttgag      420

caggtgaaaa acgctttcaa caaactgcaa gaaaagggga tctacaaagc gatgtcagag      480

ttcgacatat ttataaacta catagaagcc tatatgacga tgaaaatccg caac            534


<210>  17
<211>  302
<212>  PRT
<213>  IL-10 pentameric variant

<400>  17

Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His Glu Gly 
1               5                   10                  15      


Gly Ser Phe His Thr Asp Trp Leu Thr Leu Asn Val Gly Gly Arg Tyr 
            20                  25                  30          


Phe Thr Thr Thr Arg Ser Thr Leu Val Asn Lys Glu Pro Asp Ser Met 
        35                  40                  45              


Leu Ala His Met Phe Lys Asp Lys Gly Val Trp Gly Asn Lys Gln Asp 
    50                  55                  60                  


His Arg Gly Ala Phe Leu Ile Asp Arg Ser Pro Glu Tyr Phe Glu Pro 
65                  70                  75                  80  


Ile Leu Asn Tyr Leu Arg His Gly Gln Leu Ile Val Asn Asp Gly Ile 
                85                  90                  95      


Asn Leu Leu Gly Val Leu Glu Glu Ala Arg Phe Phe Gly Ile Asp Ser 
            100                 105                 110         


Leu Ile Glu His Leu Glu Val Ala Ile Lys Asn Ser Gly Ser Gly Gly 
        115                 120                 125             


Ser Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Ser Ser 
    130                 135                 140                 


Glu Asn Ser Cys Thr His Phe Pro Gly Ile Leu Pro Asn Met Leu Arg 
145                 150                 155                 160 


Asp Leu Arg Asp Ala Phe Ser Arg Val Lys Thr Phe Phe Gln Met Lys 
                165                 170                 175     


Asp Gln Leu Asp Asn Leu Leu Leu Lys Glu Ser Leu Leu Glu Asp Phe 
            180                 185                 190         


Lys Gly Tyr Leu Gly Cys Gln Ala Leu Ser Glu Met Ile Gln Phe Tyr 
        195                 200                 205             


Leu Glu Glu Val Met Pro Gln Ala Glu Asn Gln Asp Pro Asp Ile Lys 
    210                 215                 220                 


Ala His Val Ile Ser Leu Gly Glu Asn Leu Asn Thr Leu Arg Leu Arg 
225                 230                 235                 240 


Leu Arg Arg Cys His Arg Leu Leu Pro Cys Glu Asn Gly Gly Gly Ser 
                245                 250                 255     


Gly Gly Lys Ser Lys Ala Val Glu Gln Val Lys Asn Ala Phe Asn Lys 
            260                 265                 270         


Leu Gln Glu Lys Gly Ile Tyr Lys Ala Met Ser Glu Phe Asp Ile Phe 
        275                 280                 285             


Ile Asn Tyr Ile Glu Ala Tyr Met Thr Met Lys Ile Arg Asn 
    290                 295                 300         


<210>  18
<211>  906
<212>  DNA
<213>  Il-10 pentameric variant

<400>  18
ggtctgaacg acatcttcga ggctcagaaa atcgaatggc acgaaggtgg aagtttccac       60

acagactggc tgacattaaa tgttggaggg cggtacttta caactacacg gagcacttta      120

gtgaataaag aacctgacag tatgctggcc cacatgttta aggacaaagg tgtctgggga      180

aataagcaag atcatagagg agctttctta attgaccgaa gtcctgagta cttcgaaccc      240

attttgaact acttgcgtca tggacagctc attgtaaatg atggcattaa tttattgggt      300

gtgttagaag aagcaagatt ttttggtatt gactcattga ttgaacacct agaagtggca      360

ataaagaatt ctggttcagg tggaagtggc agcggcggtg gtggttccgg aggcggcggt      420

tctggatcct ccgagaacag ttgcacgcac tttccgggca tccttcctaa catgcttcgc      480

gacctgcgag atgcgttctc tcgagtgaaa actttctttc agatgaaaga tcagttggat      540

aatttgctcc tcaaggagtc ccttctcgaa gactttaaag gatacctggg gtgccaggcg      600

cttagcgaga tgatacagtt ctatctggag gaagtgatgc cccaagcaga aaatcaagat      660

cccgatatta aggcccatgt catcagcctt ggcgaaaatt tgaatactct cagactccga      720

cttcgccgat gccacaggct tttgccatgt gagaatgggg gtggttccgg gggtaagtcc      780

aaggccgttg agcaggtgaa aaacgctttc aacaaactgc aagaaaaggg gatctacaaa      840

gcgatgtcag agttcgacat atttataaac tacatagaag cctatatgac gatgaaaatc      900

cgcaac                                                                 906


<210>  19
<211>  308
<212>  PRT
<213>  R5A11-IL4

<400>  19

Ser Glu Asn Ser Cys Thr His Phe Pro Gly Ile Leu Pro Asn Met Leu 
1               5                   10                  15      


Arg Asp Leu Arg Asp Ala Phe Ser Arg Val Lys Thr Phe Phe Gln Met 
            20                  25                  30          


Lys Asp Gln Leu Asp Asn Leu Leu Leu Lys Glu Ser Leu Leu Glu Asp 
        35                  40                  45              


Phe Lys Gly Tyr Leu Gly Cys Gln Ala Leu Ser Glu Met Ile Gln Phe 
    50                  55                  60                  


Tyr Leu Glu Glu Val Met Pro Gln Ala Glu Asn Gln Asp Pro Asp Ile 
65                  70                  75                  80  


Lys Ala His Val Ile Ser Leu Gly Glu Asn Leu Asn Thr Leu Arg Leu 
                85                  90                  95      


Arg Leu Arg Arg Cys His Arg Leu Leu Pro Cys Glu Asn Gly Gly Gly 
            100                 105                 110         


Ser Gly Gly Lys Ser Lys Ala Val Glu Gln Val Lys Asn Ala Phe Asn 
        115                 120                 125             


Lys Leu Gln Glu Lys Gly Ile Tyr Lys Ala Met Ser Glu Phe Asp Ile 
    130                 135                 140                 


Phe Ile Asn Tyr Ile Glu Ala Tyr Met Thr Met Lys Ile Arg Asn Gly 
145                 150                 155                 160 


Ile Leu Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
                165                 170                 175     


Gly Gly Ser His Lys Cys Asp Ile Thr Leu Gln Glu Ile Ile Lys Thr 
            180                 185                 190         


Leu Asn Ser Leu Thr Glu Gln Lys Thr Leu Cys Thr Glu Leu Thr Val 
        195                 200                 205             


Thr Asp Ile Phe Ala Ala Ser Lys Asn Thr Thr Glu Lys Glu Thr Phe 
    210                 215                 220                 


Cys Arg Ala Ala Thr Val Leu Arg Gln Phe Tyr Ser His His Glu Lys 
225                 230                 235                 240 


Asp Thr Arg Cys Leu Gly Ala Thr Ala Gln Gln Phe His Arg His Lys 
                245                 250                 255     


Gln Leu Ile Arg Phe Leu Lys Arg Leu Asp Arg Asn Leu Trp Gly Leu 
            260                 265                 270         


Ala Gly Leu Asn Ser Cys Pro Val Lys Glu Ala Asn Gln Ser Thr Leu 
        275                 280                 285             


Glu Asn Phe Leu Glu Arg Leu Lys Thr Ile Met Arg Glu Lys Tyr Ser 
    290                 295                 300                 


Lys Cys Ser Ser 
305             


<210>  20
<211>  924
<212>  DNA
<213>  R5A11-IL4

<400>  20
tccgagaaca gttgcacgca ctttccgggc atccttccta acatgcttcg cgacctgcga       60

gatgcgttct ctcgagtgaa aactttcttt cagatgaaag atcagttgga taatttgctc      120

ctcaaggagt cccttctcga agactttaaa ggatacctgg ggtgccaggc gcttagcgag      180

atgatacagt tctatctgga ggaagtgatg ccccaagcag aaaatcaaga tcccgatatt      240

aaggcccatg tcatcagcct tggcgaaaat ttgaatactc tcagactccg acttcgccga      300

tgccacaggc ttttgccatg tgagaatggg ggtggttccg ggggtaagtc caaggccgtt      360

gagcaggtga aaaacgcttt caacaaactg caagaaaagg ggatctacaa agcgatgtca      420

gagttcgaca tatttataaa ctacatagaa gcctatatga cgatgaaaat ccgcaacgga      480

attctaggtt ccggtggcgg tggcagcggc ggtggtggtt ccgggggcgg cggttctcac      540

aagtgcgata tcaccttaca ggagatcatc aaaactttga acagcctcac agagcagaag      600

actctgtgca ccgagttgac cgtaacagac atctttgctg cctccaagaa cacaactgag      660

aaggaaacct tctgcagggc tgcgactgtg ctccggcagt tctacagcca ccatgagaag      720

gacactcgct gcctgggtgc gactgcacag cagttccaca ggcacaagca gctgatccga      780

ttcctgaaac ggctcgacag gaacctctgg ggcctggcgg gcttgaattc ctgtcctgtg      840

aaggaagcca accagagtac gttggaaaac ttcttggaaa ggctaaagac gatcatgaga      900

gagaaatatt caaagtgttc gagc                                             924


<210>  21
<211>  307
<212>  PRT
<213>  R5A11-IL4 (Q8A/R121A/Y124A)

<400>  21

Glu Asn Ser Cys Thr His Phe Pro Gly Ile Leu Pro Asn Met Leu Arg 
1               5                   10                  15      


Asp Leu Arg Asp Ala Phe Ser Arg Val Lys Thr Phe Phe Gln Met Lys 
            20                  25                  30          


Asp Gln Leu Asp Asn Leu Leu Leu Lys Glu Ser Leu Leu Glu Asp Phe 
        35                  40                  45              


Lys Gly Tyr Leu Gly Cys Gln Ala Leu Ser Glu Met Ile Gln Phe Tyr 
    50                  55                  60                  


Leu Glu Glu Val Met Pro Gln Ala Glu Asn Gln Asp Pro Asp Ile Lys 
65                  70                  75                  80  


Ala His Val Ile Ser Leu Gly Glu Asn Leu Asn Thr Leu Arg Leu Arg 
                85                  90                  95      


Leu Arg Arg Cys His Arg Leu Leu Pro Cys Glu Asn Gly Gly Gly Ser 
            100                 105                 110         


Gly Gly Lys Ser Lys Ala Val Glu Gln Val Lys Asn Ala Phe Asn Lys 
        115                 120                 125             


Leu Gln Glu Lys Gly Ile Tyr Lys Ala Met Ser Glu Phe Asp Ile Phe 
    130                 135                 140                 


Ile Asn Tyr Ile Glu Ala Tyr Met Thr Met Lys Ile Arg Asn Gly Ile 
145                 150                 155                 160 


Leu Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
                165                 170                 175     


Gly Ser His Lys Cys Asp Ile Thr Leu Ala Glu Ile Ile Lys Thr Leu 
            180                 185                 190         


Asn Ser Leu Thr Glu Gln Lys Thr Leu Cys Thr Glu Leu Thr Val Thr 
        195                 200                 205             


Asp Ile Phe Ala Ala Ser Lys Asn Thr Thr Glu Lys Glu Thr Phe Cys 
    210                 215                 220                 


Arg Ala Ala Thr Val Leu Arg Gln Phe Tyr Ser His His Glu Lys Asp 
225                 230                 235                 240 


Thr Arg Cys Leu Gly Ala Thr Ala Gln Gln Phe His Arg His Lys Gln 
                245                 250                 255     


Leu Ile Arg Phe Leu Lys Arg Leu Asp Arg Asn Leu Trp Gly Leu Ala 
            260                 265                 270         


Gly Leu Asn Ser Cys Pro Val Lys Glu Ala Asn Gln Ser Thr Leu Glu 
        275                 280                 285             


Asn Phe Leu Glu Arg Leu Lys Thr Ile Met Ala Glu Lys Ala Ser Lys 
    290                 295                 300                 


Cys Ser Ser 
305         


<210>  22
<211>  924
<212>  DNA
<213>  R5A11-IL4 (Q8A/R121A/Y124A)

<400>  22
tccgagaaca gttgcacgca ctttccgggc atccttccta acatgcttcg cgacctgcga       60

gatgcgttct ctcgagtgaa aactttcttt cagatgaaag atcagttgga taatttgctc      120

ctcaaggagt cccttctcga agactttaaa ggatacctgg ggtgccaggc gcttagcgag      180

atgatacagt tctatctgga ggaagtgatg ccccaagcag aaaatcaaga tcccgatatt      240

aaggcccatg tcatcagcct tggcgaaaat ttgaatactc tcagactccg acttcgccga      300

tgccacaggc ttttgccatg tgagaatggg ggtggttccg ggggtaagtc caaggccgtt      360

gagcaggtga aaaacgcttt caacaaactg caagaaaagg ggatctacaa agcgatgtca      420

gagttcgaca tatttataaa ctacatagaa gcctatatga cgatgaaaat ccgcaacgga      480

attctaggtt ccggtggcgg tggcagcggc ggtggtggtt ccgggggcgg cggttctcac      540

aagtgcgata tcaccttagc ggagatcatc aaaactttga acagcctcac agagcagaag      600

actctgtgca ccgagttgac cgtaacagac atctttgctg cctccaagaa cacaactgag      660

aaggaaacct tctgcagggc tgcgactgtg ctccggcagt tctacagcca ccatgagaag      720

gacactcgct gcctgggtgc gactgcacag cagttccaca ggcacaagca gctgatccga      780

ttcctgaaac ggctcgacag gaacctctgg ggcctggcgg gcttgaattc ctgtcctgtg      840

aaggaagcca accagagtac gttggaaaac ttcttggaaa ggctaaagac gatcatggca      900

gagaaagctt caaagtgttc gagc                                             924


<210>  23
<211>  307
<212>  PRT
<213>  R5A11-IL4 (E12A/K84A)

<400>  23

Glu Asn Ser Cys Thr His Phe Pro Gly Ile Leu Pro Asn Met Leu Arg 
1               5                   10                  15      


Asp Leu Arg Asp Ala Phe Ser Arg Val Lys Thr Phe Phe Gln Met Lys 
            20                  25                  30          


Asp Gln Leu Asp Asn Leu Leu Leu Lys Glu Ser Leu Leu Glu Asp Phe 
        35                  40                  45              


Lys Gly Tyr Leu Gly Cys Gln Ala Leu Ser Glu Met Ile Gln Phe Tyr 
    50                  55                  60                  


Leu Glu Glu Val Met Pro Gln Ala Glu Asn Gln Asp Pro Asp Ile Lys 
65                  70                  75                  80  


Ala His Val Ile Ser Leu Gly Glu Asn Leu Asn Thr Leu Arg Leu Arg 
                85                  90                  95      


Leu Arg Arg Cys His Arg Leu Leu Pro Cys Glu Asn Gly Gly Gly Ser 
            100                 105                 110         


Gly Gly Lys Ser Lys Ala Val Glu Gln Val Lys Asn Ala Phe Asn Lys 
        115                 120                 125             


Leu Gln Glu Lys Gly Ile Tyr Lys Ala Met Ser Glu Phe Asp Ile Phe 
    130                 135                 140                 


Ile Asn Tyr Ile Glu Ala Tyr Met Thr Met Lys Ile Arg Asn Gly Ile 
145                 150                 155                 160 


Leu Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
                165                 170                 175     


Gly Ser His Lys Cys Asp Ile Thr Leu Gln Ala Ile Ile Lys Thr Leu 
            180                 185                 190         


Asn Ser Leu Thr Glu Gln Lys Thr Leu Cys Thr Glu Leu Thr Val Thr 
        195                 200                 205             


Asp Ile Phe Ala Ala Ser Lys Asn Thr Thr Glu Lys Glu Thr Phe Cys 
    210                 215                 220                 


Arg Ala Ala Thr Val Leu Arg Gln Phe Tyr Ser His His Glu Lys Asp 
225                 230                 235                 240 


Thr Arg Cys Leu Gly Ala Thr Ala Gln Gln Phe His Arg His Lys Gln 
                245                 250                 255     


Leu Ile Arg Phe Leu Ala Arg Leu Asp Arg Asn Leu Trp Gly Leu Ala 
            260                 265                 270         


Gly Leu Asn Ser Cys Pro Val Lys Glu Ala Asn Gln Ser Thr Leu Glu 
        275                 280                 285             


Asn Phe Leu Glu Arg Leu Lys Thr Ile Met Arg Glu Lys Tyr Ser Lys 
    290                 295                 300                 


Cys Ser Ser 
305         


<210>  24
<211>  924
<212>  DNA
<213>  R5A11-IL4 (E12A/K84A)

<400>  24
tccgagaaca gttgcacgca ctttccgggc atccttccta acatgcttcg cgacctgcga       60

gatgcgttct ctcgagtgaa aactttcttt cagatgaaag atcagttgga taatttgctc      120

ctcaaggagt cccttctcga agactttaaa ggatacctgg ggtgccaggc gcttagcgag      180

atgatacagt tctatctgga ggaagtgatg ccccaagcag aaaatcaaga tcccgatatt      240

aaggcccatg tcatcagcct tggcgaaaat ttgaatactc tcagactccg acttcgccga      300

tgccacaggc ttttgccatg tgagaatggg ggtggttccg ggggtaagtc caaggccgtt      360

gagcaggtga aaaacgcttt caacaaactg caagaaaagg ggatctacaa agcgatgtca      420

gagttcgaca tatttataaa ctacatagaa gcctatatga cgatgaaaat ccgcaacgga      480

attctaggtt ccggtggcgg tggcagcggc ggtggtggtt ccgggggcgg cggttctcac      540

aagtgcgata tcaccttaca ggctatcatc aaaactttga acagcctcac agagcagaag      600

actctgtgca ccgagttgac cgtaacagac atctttgctg cctccaagaa cacaactgag      660

aaggaaacct tctgcagggc tgcgactgtg ctccggcagt tctacagcca ccatgagaag      720

gacactcgct gcctgggtgc gactgcacag cagttccaca ggcacaagca gctgatccga      780

ttcctggctc ggctcgacag gaacctctgg ggcctggcgg gcttgaattc ctgtcctgtg      840

aaggaagcca accagagtac gttggaaaac ttcttggaaa ggctaaagac gatcatgaga      900

gagaaatatt caaagtgttc gagc                                             924


