                         SEQUENCE LISTING

<110>  Telum Therapeutics S.L.
 
<120>  NEW RECOMBINANT LYSIN AND ITS USE IN THE TREATMENT OF GRAM-NEGATIVE BACTERIAL INFECTIONS

<130>  906 002

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  163
<212>  PRT
<213>  Enterobacter phage Arya


<220>
<221>  MISC_FEATURE
<222>  (1)..(163)
<223>  Endolysin

<400>  1

Met Lys Thr Ser Pro Asn Gly Ile Ala Val Thr Lys Tyr Phe Glu Ser 
1               5                   10                  15      


Phe Glu Ala Arg Ala Tyr Pro Asp Pro Ala Thr Gly Gly Lys Pro Tyr 
            20                  25                  30          


Thr Ile Gly Phe Gly Thr Thr Val Tyr Pro Ser Gly Ala Pro Val Arg 
        35                  40                  45              


Leu Gly Asp Val Cys Thr Lys Glu Gln Ala Glu Lys Tyr Leu Gln Asn 
    50                  55                  60                  


Asp Leu Ala Lys Phe Glu Lys Ile Val Ser Asp Ala Val Arg Val Pro 
65                  70                  75                  80  


Leu Asn Gln Gly Gln Phe Asp Ala Leu Val Ser Phe Thr Tyr Asn Leu 
                85                  90                  95      


Gly Pro Ala Asn Leu Arg Ser Ser Thr Leu Leu Lys Lys Leu Asn Ala 
            100                 105                 110         


Gly Asp Tyr Ala Gly Ala Ala Lys Glu Phe Pro Arg Trp Asn Arg Ala 
        115                 120                 125             


Asn Gly Lys Val Met Lys Gly Leu Thr Arg Arg Arg Ala Ala Glu Gln 
    130                 135                 140                 


Cys Leu Phe Glu Gly Met Gly Gly Ala Ser Ala Ile Glu Arg Gly Val 
145                 150                 155                 160 


Ala Ala Ala 
            


<210>  2
<211>  116
<212>  PRT
<213>  Bacillus amyloliquefaciens phage


<220>
<221>  MISC_FEATURE
<222>  (1)..(116)
<223>  cell permeability domain (D8)

<400>  2

Asn Ser Gly Thr Pro Lys Asn Val Ser Arg Gly Thr Ser Ser Thr Lys 
1               5                   10                  15      


Thr Thr Pro Lys Tyr Lys Val Lys Asn Gly Asp Asn Leu Thr Lys Ile 
            20                  25                  30          


Ala Lys Lys His Asn Thr Thr Val Ala Thr Leu Leu Lys Leu Asn Pro 
        35                  40                  45              


Gly Ile Lys Asp Pro Asn Met Ile Arg Val Gly Gln Thr Leu Asn Val 
    50                  55                  60                  


Thr Gly Ser Gly Gly Lys Thr His Lys Val Lys Ser Gly Asp Thr Leu 
65                  70                  75                  80  


Ser Lys Ile Ala Val Asp Asn Lys Thr Thr Val Ser Lys Leu Met Asn 
                85                  90                  95      


Leu Asn Pro Glu Ile Thr Asn Pro Asn His Ile Lys Val Gly Gln Thr 
            100                 105                 110         


Ile Arg Leu Ser 
        115     


<210>  3
<211>  279
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chimeric endolysin (SEQ ID NO:1 + SEQ ID NO:2)


<220>
<221>  MISC_FEATURE
<222>  (1)..(179)

<400>  3

Met Lys Thr Ser Pro Asn Gly Ile Ala Val Thr Lys Tyr Phe Glu Ser 
1               5                   10                  15      


Phe Glu Ala Arg Ala Tyr Pro Asp Pro Ala Thr Gly Gly Lys Pro Tyr 
            20                  25                  30          


Thr Ile Gly Phe Gly Thr Thr Val Tyr Pro Ser Gly Ala Pro Val Arg 
        35                  40                  45              


Leu Gly Asp Val Cys Thr Lys Glu Gln Ala Glu Lys Tyr Leu Gln Asn 
    50                  55                  60                  


Asp Leu Ala Lys Phe Glu Lys Ile Val Ser Asp Ala Val Arg Val Pro 
65                  70                  75                  80  


Leu Asn Gln Gly Gln Phe Asp Ala Leu Val Ser Phe Thr Tyr Asn Leu 
                85                  90                  95      


Gly Pro Ala Asn Leu Arg Ser Ser Thr Leu Leu Lys Lys Leu Asn Ala 
            100                 105                 110         


Gly Asp Tyr Ala Gly Ala Ala Lys Glu Phe Pro Arg Trp Asn Arg Ala 
        115                 120                 125             


Asn Gly Lys Val Met Lys Gly Leu Thr Arg Arg Arg Ala Ala Glu Gln 
    130                 135                 140                 


Cys Leu Phe Glu Gly Met Gly Gly Ala Ser Ala Ile Glu Arg Gly Val 
145                 150                 155                 160 


Ala Ala Ala Asn Ser Gly Thr Pro Lys Asn Val Ser Arg Gly Thr Ser 
                165                 170                 175     


Ser Thr Lys Thr Thr Pro Lys Tyr Lys Val Lys Asn Gly Asp Asn Leu 
            180                 185                 190         


Thr Lys Ile Ala Lys Lys His Asn Thr Thr Val Ala Thr Leu Leu Lys 
        195                 200                 205             


Leu Asn Pro Gly Ile Lys Asp Pro Asn Met Ile Arg Val Gly Gln Thr 
    210                 215                 220                 


Leu Asn Val Thr Gly Ser Gly Gly Lys Thr His Lys Val Lys Ser Gly 
225                 230                 235                 240 


Asp Thr Leu Ser Lys Ile Ala Val Asp Asn Lys Thr Thr Val Ser Lys 
                245                 250                 255     


Leu Met Asn Leu Asn Pro Glu Ile Thr Asn Pro Asn His Ile Lys Val 
            260                 265                 270         


Gly Gln Thr Ile Arg Leu Ser 
        275                 


<210>  4
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  thrombin cutting site


<220>
<221>  MISC_FEATURE
<222>  (1)..(6)

<400>  4

Leu Val Pro Arg Gly Ser 
1               5       


<210>  5
<211>  329
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chimeric endolysin (IKB207)


<220>
<221>  MISC_FEATURE
<222>  (1)..(329)

<400>  5

Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln His Met Asp Ser 
1               5                   10                  15      


Pro Asp Leu Gly Thr Leu Val Pro Arg Gly Ser Met Ala Ile Ser Asp 
            20                  25                  30          


Pro Met Lys Thr Ser Pro Asn Gly Ile Ala Val Thr Lys Tyr Phe Glu 
        35                  40                  45              


Ser Phe Glu Ala Arg Ala Tyr Pro Asp Pro Ala Thr Gly Gly Lys Pro 
    50                  55                  60                  


Tyr Thr Ile Gly Phe Gly Thr Thr Val Tyr Pro Ser Gly Ala Pro Val 
65                  70                  75                  80  


Arg Leu Gly Asp Val Cys Thr Lys Glu Gln Ala Glu Lys Tyr Leu Gln 
                85                  90                  95      


Asn Asp Leu Ala Lys Phe Glu Lys Ile Val Ser Asp Ala Val Arg Val 
            100                 105                 110         


Pro Leu Asn Gln Gly Gln Phe Asp Ala Leu Val Ser Phe Thr Tyr Asn 
        115                 120                 125             


Leu Gly Pro Ala Asn Leu Arg Ser Ser Thr Leu Leu Lys Lys Leu Asn 
    130                 135                 140                 


Ala Gly Asp Tyr Ala Gly Ala Ala Lys Glu Phe Pro Arg Trp Asn Arg 
145                 150                 155                 160 


Ala Asn Gly Lys Val Met Lys Gly Leu Thr Arg Arg Arg Ala Ala Glu 
                165                 170                 175     


Gln Cys Leu Phe Glu Gly Met Gly Gly Ala Ser Ala Ile Glu Arg Gly 
            180                 185                 190         


Val Ala Ala Ala Asn Ser Gly Thr Pro Lys Asn Val Ser Arg Gly Thr 
        195                 200                 205             


Ser Ser Thr Lys Thr Thr Pro Lys Tyr Lys Val Lys Asn Gly Asp Asn 
    210                 215                 220                 


Leu Thr Lys Ile Ala Lys Lys His Asn Thr Thr Val Ala Thr Leu Leu 
225                 230                 235                 240 


Lys Leu Asn Pro Gly Ile Lys Asp Pro Asn Met Ile Arg Val Gly Gln 
                245                 250                 255     


Thr Leu Asn Val Thr Gly Ser Gly Gly Lys Thr His Lys Val Lys Ser 
            260                 265                 270         


Gly Asp Thr Leu Ser Lys Ile Ala Val Asp Asn Lys Thr Thr Val Ser 
        275                 280                 285             


Lys Leu Met Asn Leu Asn Pro Glu Ile Thr Asn Pro Asn His Ile Lys 
    290                 295                 300                 


Val Gly Gln Thr Ile Arg Leu Ser Leu Gly Thr Leu Val Pro Arg Gly 
305                 310                 315                 320 


Ser Leu Glu His His His His His His 
                325                 


<210>  6
<211>  489
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  polynucleotide encoding SEQ ID NO:1


<220>
<221>  misc_feature
<222>  (1)..(489)

<400>  6
atgaaaacct ctccaaatgg tatcgccgtt accaagtact tcgaatcatt tgaagcccgc       60

gcataccctg accccgccac tggcggtaaa ccatacacga ttggcttcgg aaccactgtc      120

tacccgtctg gcgcacccgt ccgtttaggg gatgtgtgta cgaaagaaca ggccgagaaa      180

tatttacaaa atgacttggc gaaattcgag aagattgtat ctgacgcagt gcgcgttccc      240

cttaatcaag gtcagtttga cgcgttagtg tcatttacgt ataacttagg acccgccaat      300

ttgcgcagca gtaccctgtt aaaaaagttg aacgctgggg actatgcggg ggccgctaaa      360

gagtttccgc gttggaaccg tgcaaacggt aaagtgatga aaggtttgac acgtcgccgc      420

gcggcagaac aatgtttgtt tgaagggatg ggaggcgcga gcgcgattga acgtggtgta      480

gccgctgca                                                              489


<210>  7
<211>  348
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding a polypeptide of SEQ ID NO:2


<220>
<221>  misc_feature
<222>  (1)..(348)

<400>  7
aacagtggga caccaaagaa tgtttcccgc ggaacctcgt ccacgaagac aacacctaag       60

tataaggtaa aaaatggtga caacttaact aaaatcgcga agaaacataa tactacagta      120

gcgacattgc tgaaacttaa tccagggatc aaagacccca acatgattcg tgtagggcag      180

actttaaatg ttacagggtc cggtgggaaa actcataaag tcaagtcggg tgacacactg      240

agtaaaatcg cagttgataa taagacgact gttagcaagt tgatgaatct taacccggaa      300

atcactaatc ctaaccatat caaagtcggc cagacaatcc gtttgagc                   348


<210>  8
<211>  837
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide sequence encoding SEQ ID NO:3


<220>
<221>  misc_feature
<222>  (1)..(837)

<400>  8
atgaaaacct ctccaaatgg tatcgccgtt accaagtact tcgaatcatt tgaagcccgc       60

gcataccctg accccgccac tggcggtaaa ccatacacga ttggcttcgg aaccactgtc      120

tacccgtctg gcgcacccgt ccgtttaggg gatgtgtgta cgaaagaaca ggccgagaaa      180

tatttacaaa atgacttggc gaaattcgag aagattgtat ctgacgcagt gcgcgttccc      240

cttaatcaag gtcagtttga cgcgttagtg tcatttacgt ataacttagg acccgccaat      300

ttgcgcagca gtaccctgtt aaaaaagttg aacgctgggg actatgcggg ggccgctaaa      360

gagtttccgc gttggaaccg tgcaaacggt aaagtgatga aaggtttgac acgtcgccgc      420

gcggcagaac aatgtttgtt tgaagggatg ggaggcgcga gcgcgattga acgtggtgta      480

gccgctgcaa acagtgggac accaaagaat gtttcccgcg gaacctcgtc cacgaagaca      540

acacctaagt ataaggtaaa aaatggtgac aacttaacta aaatcgcgaa gaaacataat      600

actacagtag cgacattgct gaaacttaat ccagggatca aagaccccaa catgattcgt      660

gtagggcaga ctttaaatgt tacagggtcc ggtgggaaaa ctcataaagt caagtcgggt      720

gacacactga gtaaaatcgc agttgataat aagacgactg ttagcaagtt gatgaatctt      780

aacccggaaa tcactaatcc taaccatatc aaagtcggcc agacaatccg tttgagc         837


<210>  9
<211>  987
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  polynucleotide encoding SEQ ID NO:5


<220>
<221>  misc_feature
<222>  (1)..(987)

<400>  9
atgaaagaaa ccgctgctgc taaattcgaa cgccagcaca tggacagccc agatctgggt       60

accctggtgc cacgcggttc catggcgata tcggatccga tgaaaacctc tccaaatggt      120

atcgccgtta ccaagtactt cgaatcattt gaagcccgcg cataccctga ccccgccact      180

ggcggtaaac catacacgat tggcttcgga accactgtct acccgtctgg cgcacccgtc      240

cgtttagggg atgtgtgtac gaaagaacag gccgagaaat atttacaaaa tgacttggcg      300

aaattcgaga agattgtatc tgacgcagtg cgcgttcccc ttaatcaagg tcagtttgac      360

gcgttagtgt catttacgta taacttagga cccgccaatt tgcgcagcag taccctgtta      420

aaaaagttga acgctgggga ctatgcgggg gccgctaaag agtttccgcg ttggaaccgt      480

gcaaacggta aagtgatgaa aggtttgaca cgtcgccgcg cggcagaaca atgtttgttt      540

gaagggatgg gaggcgcgag cgcgattgaa cgtggtgtag ccgctgcaaa cagtgggaca      600

ccaaagaatg tttcccgcgg aacctcgtcc acgaagacaa cacctaagta taaggtaaaa      660

aatggtgaca acttaactaa aatcgcgaag aaacataata ctacagtagc gacattgctg      720

aaacttaatc cagggatcaa agaccccaac atgattcgtg tagggcagac tttaaatgtt      780

acagggtccg gtgggaaaac tcataaagtc aagtcgggtg acacactgag taaaatcgca      840

gttgataata agacgactgt tagcaagttg atgaatctta acccggaaat cactaatcct      900

aaccatatca aagtcggcca gacaatccgt ttgagcctgg gtaccctggt gccacgcggt      960

tccctcgagc accaccacca ccaccac                                          987


