                         SEQUENCE LISTING

<110>  TAIPEI UNIVERSITY OF TECHNOLOGY
 
<120>  METHODS OF DETECTION OF COMPOUND, ANTIBODY OR PROTEIN USING 
       RECOMBINANT ENDOSPORES OR BACTERIA AS SENSING ELEMENT

<130>  Q253724

<150>  US 16/813,028
<151>  2020-03-09

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  380
<212>  PRT
<213>  Bacillus subtilis

<400>  1

Met Ser Lys Arg Arg Met Lys Tyr His Ser Asn Asn Glu Ile Ser Tyr 
1               5                   10                  15      


Tyr Asn Phe Leu His Ser Met Lys Asp Lys Ile Val Thr Val Tyr Arg 
            20                  25                  30          


Gly Gly Pro Glu Ser Lys Lys Gly Lys Leu Thr Ala Val Lys Ser Asp 
        35                  40                  45              


Tyr Ile Ala Leu Gln Ala Glu Lys Lys Ile Ile Tyr Tyr Gln Leu Glu 
    50                  55                  60                  


His Val Lys Ser Ile Thr Glu Asp Thr Asn Asn Ser Thr Thr Thr Ile 
65                  70                  75                  80  


Glu Thr Glu Glu Met Leu Asp Ala Asp Asp Phe His Ser Leu Ile Gly 
                85                  90                  95      


His Leu Ile Asn Gln Ser Val Gln Phe Asn Gln Gly Gly Pro Glu Ser 
            100                 105                 110         


Lys Lys Gly Arg Leu Val Trp Leu Gly Asp Asp Tyr Ala Ala Leu Asn 
        115                 120                 125             


Thr Asn Glu Asp Gly Val Val Tyr Phe Asn Ile His His Ile Lys Ser 
    130                 135                 140                 


Ile Ser Lys His Glu Pro Asp Leu Lys Ile Glu Glu Gln Thr Pro Val 
145                 150                 155                 160 


Gly Val Leu Glu Ala Asp Asp Leu Ser Glu Val Phe Lys Ser Leu Thr 
                165                 170                 175     


His Lys Trp Val Ser Ile Asn Arg Gly Gly Pro Glu Ala Ile Glu Gly 
            180                 185                 190         


Ile Leu Val Asp Asn Ala Asp Gly His Tyr Thr Ile Val Lys Asn Gln 
        195                 200                 205             


Glu Val Leu Arg Ile Tyr Pro Phe His Ile Lys Ser Ile Ser Leu Gly 
    210                 215                 220                 


Pro Lys Gly Ser Tyr Lys Lys Glu Asp Gln Lys Asn Glu Gln Asn Gln 
225                 230                 235                 240 


Glu Asp Asn Asn Asp Lys Asp Ser Asn Ser Phe Ile Ser Ser Lys Ser 
                245                 250                 255     


Tyr Ser Ser Ser Lys Ser Ser Lys Arg Ser Leu Lys Ser Ser Asp Asp 
            260                 265                 270         


Gln Ser Ser Lys Ser Gly Arg Ser Ser Arg Ser Lys Ser Ser Ser Lys 
        275                 280                 285             


Ser Ser Lys Arg Ser Leu Lys Ser Ser Asp Tyr Gln Ser Ser Lys Ser 
    290                 295                 300                 


Gly Arg Ser Ser Arg Ser Lys Ser Ser Ser Lys Ser Ser Lys Arg Ser 
305                 310                 315                 320 


Leu Lys Ser Ser Asp Tyr Gln Ser Ser Lys Ser Ser Lys Arg Ser Pro 
                325                 330                 335     


Arg Ser Ser Asp Tyr Gln Ser Ser Arg Ser Pro Gly Tyr Ser Ser Ser 
            340                 345                 350         


Ile Lys Ser Ser Gly Lys Gln Lys Glu Asp Tyr Ser Tyr Glu Thr Ile 
        355                 360                 365             


Val Arg Thr Ile Asp Tyr His Trp Lys Arg Lys Phe 
    370                 375                 380 


<210>  2
<211>  1226
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  2
ctgcagaaat cgtttgggcc gatgaaaaat cggctcttta ttttgatttg tttttgtgtc       60

atctgtcttt ttctatcatt tggacagccc ttttttcctt ctatgatttt aactgtccaa      120

gccgcaaaat ctactcgccg tataataaag cgtagtaaaa ataaaggagg agtatatatg      180

ggttattaca aaaaatacaa agaagagtat tatacggtca aaaaaacgta ttataagaag      240

tattacgaat atgataaaaa agattatgac tgtgattacg acaaaaaata tgatgactat      300

gataaaaaat attatgatca cgataaaaaa gactatgatt atgttgtaga gtataaaaag      360

cataaaaaac actacgcagc tgcacttgca cttgcagcac tactagcact agctctagct      420

atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac      480

ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac      540

ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc      600

ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag      660

cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc      720

ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg      780

gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac      840

aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac      900

ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc      960

gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac     1020

tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc     1080

ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa     1140

gcggcctaaa cgccattaac atctcctcgt ttttactttc ccccggctat tgccgggtct     1200

tttttgtttg tgcactatat ctcgag                                          1226


<210>  3
<211>  3147
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  3
aagcttcaat gaaaaaaggg cccgcaggcc ctttgttcga tatcaatcga gattatgaca       60

acttgacggc tacatcattc actttttctt cacaaccggc acggaactcg ctcgggctgg      120

ccccggtgca ttttttaaat acccgcgaga aatagagttg atcgtcaaaa ccaacattgc      180

gaccgacggt ggcgataggc atccgggtgg tgctcaaaag cagcttcgcc tggctgatac      240

gttggtcctc gcgccagctt aagacgctaa tccctaactg ctggcggaaa agatgtgaca      300

gacgcgacgg cgacaagcaa acatgctgtg cgacgctggc gatatcaaaa ttgctgtctg      360

ccaggtgatc gctgatgtac tgacaagcct cgcgtacccg attatccatc ggtggatgga      420

gcgactcgtt aatcgcttcc atgcgccgca gtaacaattg ctcaagcaga tttatcgcca      480

gcagctccga atagcgccct tccccttgcc cggcgttaat gatttgccca aacaggtcgc      540

tgaaatgcgg ctggtgcgct tcatccgggc gaaagaaccc cgtattggca aatattgacg      600

gccagttaag ccattcatgc cagtaggcgc gcggacgaaa gtaaacccac tggtgatacc      660

attcgcgagc ctccggatga cgaccgtagt gatgaatctc tcctggcggg aacagcaaaa      720

tatcacccgg tcggcaaaca aattctcgtc cctgattttt caccaccccc tgaccgcgaa      780

tggtgagatt gagaatataa cctttcattc ccagcggtcg gtcgataaaa aaatcgagat      840

aaccgttggc ctcaatcggc gttaaacccg ccaccagatg ggcattaaac gagtatcccg      900

gcagcagggg atcattttgc gcttcagcca tacttttcat actcccgcca ttcagagaag      960

aaaccaattg tccatattgc atcagacatt gccgtcactg cgtcttttac tggctcttct     1020

cgctaaccaa accggtaacc ccgcttatta aaagcattct gtaacaaagc gggaccaaag     1080

ccatgacaaa aacgcgtaac aaaagtgtct ataatcacgg cagaaaagtc cacattgatt     1140

atttgcacgg cgtcacactt tgctatgcca tagcattttt atccataaga ttagcggatc     1200

ctacctgacg ctttttatcg caactctcta ctgtttctcc atacccgttt ttttggacat     1260

gtaggaggaa ttcacatgaa agcaaccaag ctggttctgg gtgccgtgat tctgggcagt     1320

accctgttag caggttgttc tagcaatgcc aaaatcgacc aaggcatcaa caacaatggc     1380

ccgacccacg aaaaccagct gggtgccggt gcctttggtg gttatcaggt gaacccgtac     1440

gtgggctttg aaatgggcta tgattggctg ggccgcatgc cgtacaaagg cagtgtggag     1500

aacggcgcct ataaagcaca gggcgtgcag ctgacagcaa aactgggcta ccctattacc     1560

gacgacctgg acatctacac acgcttaggc ggcatggtgt ggcgcgccga taccaagagc     1620

aacgtgtacg gcaagaacca cgataccggc gtgagtccgg tgtttgccgg cggtgtggag     1680

tatgcaatca ccccggaaat tgccacacgt ggaattgcta gcggtacccc cggggccgag     1740

gccggcatca ccggcacctg gtacaaccag ctcggctcga ccttcatcgt gaccgcgggc     1800

gccgacggcg ccctgaccgg aacctacgag tcggccgtcg gcaacgccga gagccgctac     1860

gtcctgaccg gtcgttacga cagcgccccg gccaccgacg gcagcggcac cgccctcggt     1920

tggacggtgg cctggaagaa taactaccgc aacgcccact ccgcgaccac gtggagcggc     1980

cagtacgtcg gcggcgccga ggcgaggatc aacacccagt ggctgctgac ctccggcacc     2040

accgaggcca acgcctggaa gtccacgctg gtcggccacg acaccttcac caaggtgaag     2100

ccgtccgccg cctccatcta gtgactgtat aaaaccacag ccaatcaaac gaaactcgag     2160

cgatcggacg tcccaggcta tactcaagcc tggttttttg atggctgcag catatgtcta     2220

gacgtatttt acactttatg cttccggctc gtataatgtg tggaattgtg agcggataac     2280

aatttcacac aggagacagc tatggtgagc aagggcgagg agctgttcac cggggtggtg     2340

cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag     2400

ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag     2460

ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc     2520

cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac     2580

gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg     2640

aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag     2700

gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc     2760

atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag     2820

gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc     2880

gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac     2940

gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc     3000

atggacgagc tgtacaagta acaaagcccg aaaggaagct gagttggctg ctgccaccgc     3060

tgagcaataa cctagggtcg acgagctcct agcataaccc cttggggcct ctaaacgggt     3120

cttgaggggt tttttgtcta caagctt                                         3147


<210>  4
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  4
caatgaaaaa agggcccgca                                                   20


<210>  5
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  5
tcttccgctt cctcgctca                                                    19


<210>  6
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  6
cgtcgtcgtc gtcgtcgatc g                                                 21


<210>  7
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  7
cgatcgcgtc gtcgtcgtcg tcgtcgtcgt cgtcgt                                 36


