                         SEQUENCE LISTING

<110>  Commonwealth Scientific and Industrial Research 
       Organisation
       Nourish Ingredients Pty Ltd
 
<120>  Production of short chain fatty acids

<130>  531934PCT

<150>  AU 2020900780
<151>  2020-03-13

<160>  30    

<170>  PatentIn version 3.5

<210>  1
<211>  363
<212>  PRT
<213>  Euonymus alatus

<400>  1

Met Met Asp Ala His Gln Glu Ile Lys Asn Phe Ile Lys Val Trp Val 
1               5                   10                  15      


Gln Ala Met Val Cys Leu Ser Tyr Ala Tyr Tyr Phe Ser Ser Arg Leu 
            20                  25                  30          


Pro Lys Gly Leu Leu Arg Leu Leu Ser Leu Leu Pro Val Leu Tyr Leu 
        35                  40                  45              


Leu Leu Ile Ala Pro Leu Asn Ile Ser Ser Phe Ile Leu Ser Ser Ile 
    50                  55                  60                  


Thr Gly Phe Phe Leu Ala Trp Leu Thr Thr Phe Lys Val Ile Ser Phe 
65                  70                  75                  80  


Ala Phe Asp Gln Gly Pro Leu Tyr Pro Leu Pro Gln Asn Leu Leu His 
                85                  90                  95      


Phe Ile Ser Ile Ala Cys Leu Pro Ile Thr Ile Lys Arg Asn Pro Ser 
            100                 105                 110         


Pro Lys Leu Lys Ser Thr Thr Asn Pro Ser Pro Ile Ser His Leu Leu 
        115                 120                 125             


Lys Lys Ala Phe Met Ser Phe Pro Ser Lys Val Leu Phe His Trp Val 
    130                 135                 140                 


Ile Ala His Leu Tyr Gln Tyr Lys Lys Tyr Met Asp Pro Asn Val Val 
145                 150                 155                 160 


Leu Val Ile Tyr Cys Cys His Val Tyr Val Met Leu Asp Ile Ser Leu 
                165                 170                 175     


Ser Leu Cys Ala Thr Leu Ala Glu Phe Leu Cys Gly Phe Asp Val Glu 
            180                 185                 190         


Pro Gln Phe Lys Glu Pro Tyr Leu Ala Thr Ser Leu Gln Asp Phe Trp 
        195                 200                 205             


Gly Arg Arg Trp Asn Ile Ile Val Ser Ser Val Leu Arg Ser Thr Val 
    210                 215                 220                 


Tyr Ala Pro Thr Arg Asn Ile Ala Ser Tyr Leu Ile Gly Ser Arg Trp 
225                 230                 235                 240 


Ala Tyr Phe Pro Ala Ile Ile Ala Thr Phe Val Val Ser Gly Val Met 
                245                 250                 255     


His Asp Val Val Tyr Tyr Val Tyr Met Met His Met Tyr Pro Lys Trp 
            260                 265                 270         


Asp Met Thr Gly His Phe Val Leu His Gly Ile Cys Glu Ala Leu Glu 
        275                 280                 285             


Val Glu Met Lys Cys Lys Arg Ser Arg Ser Asp Lys Trp Arg Arg His 
    290                 295                 300                 


Pro Ala Val Asp Trp Val Met Val Met Gly Phe Val Met Gly Thr Ser 
305                 310                 315                 320 


Val Ser Leu Leu Phe Val Pro Leu Leu Arg Asp Asn Val Asp Gln Ile 
                325                 330                 335     


Val Ala Glu Glu Tyr Ser Ile Leu Phe Asn Phe Val Arg Glu Lys Ile 
            340                 345                 350         


Val Met Leu Gly Thr Arg Phe Val Cys Gly Asn 
        355                 360             


<210>  2
<211>  1092
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of the protein coding region of Euonymus 
       alatus 1,2-diacyl-sn-glycerol:acetyl-CoA acetyltransferase 
       (EaDAcT) in pAT001, codon optimised for expression in yeast.

<400>  2
atgatggacg cccaccaaga gatcaagaac tttattaagg tttgggttca agccatggtc       60

tgtttgtctt atgcttacta cttcagctct agattgccaa aaggtttgtt gaggttgttg      120

tctttgttgc cagtcttgta cttgttgttg attgccccat tgaacatctc gtccttcatt      180

ttgtcatcta tcaccggttt tttcttggct tggttgacta ccttcaaggt tatttctttc      240

gcttttgatc agggtccatt atacccattg ccacaaaatt tgttgcactt catttccatt      300

gcctgcttgc caattaccat taagagaaat ccatctccaa agctgaagtc tactactaat      360

ccatcaccaa tctctcactt gttgaaaaag gctttcatga gcttcccatc caaggttttg      420

tttcattggg ttattgctca cctgtaccag tacaaaaagt acatggatcc aaacgttgtc      480

ttggttatct actgttgcca tgtttacgtt atgctggaca tctctttgtc attgtgtgct      540

actttggctg aattcttgtg tggttttgat gtcgaaccac agttcaaaga accatacttg      600

gctacttcat tgcaagattt ttggggtaga cgttggaaca tcatcgtttc ttcagttttg      660

agatctaccg tttacgctcc aactagaaac attgcctctt acttgattgg ttctagatgg      720

gcttatttcc cagctattat tgctaccttt gttgtctctg gtgttatgca cgatgttgtt      780

tactacgttt acatgatgca catgtaccca aagtgggata tgactggtca tttcgtttta      840

catggtattt gcgaagcctt ggaagttgag atgaagtgta aaagatccag atctgataag      900

tggcgtagac atccagctgt tgattgggta atggttatgg gttttgttat gggtacttcc      960

gtcagcttgt tgtttgttcc attattgaga gataacgtcg atcaaatcgt tgccgaagaa     1020

tactccattt tgttcaactt cgtcagggaa aagatcgtca tgttgggtac tagatttgtt     1080

tgcggtaact aa                                                         1092


<210>  3
<211>  489
<212>  PRT
<213>  Capra hircus

<400>  3

Met Gly Asp Arg Gly Gly Ala Gly Gly Ser Arg Arg Arg Arg Thr Gly 
1               5                   10                  15      


Ser Arg Pro Ser Ile Gln Gly Gly Ser Arg Pro Ala Ala Ala Glu Glu 
            20                  25                  30          


Glu Val Arg Asp Val Gly Ala Gly Gly Asp Ala Pro Val Arg Asp Thr 
        35                  40                  45              


Asp Lys Asp Gly Asp Val Asp Val Gly Ser Gly His Trp Asp Leu Arg 
    50                  55                  60                  


Cys His Arg Leu Gln Asp Ser Leu Phe Ser Ser Asp Ser Gly Phe Ser 
65                  70                  75                  80  


Asn Tyr Arg Gly Ile Leu Asn Trp Cys Val Val Met Leu Ile Leu Ser 
                85                  90                  95      


Asn Ala Arg Leu Phe Leu Glu Asn Leu Ile Lys Tyr Gly Ile Leu Val 
            100                 105                 110         


Asp Pro Ile Gln Val Val Ser Leu Phe Leu Lys Asp Pro Tyr Ser Trp 
        115                 120                 125             


Pro Ala Leu Cys Leu Val Ile Val Ala Asn Ile Phe Ala Val Ala Ala 
    130                 135                 140                 


Phe Gln Val Glu Lys Arg Leu Ala Val Gly Ala Leu Thr Glu Gln Ala 
145                 150                 155                 160 


Gly Leu Leu Leu His Gly Val Asn Leu Ala Thr Ile Leu Cys Phe Pro 
                165                 170                 175     


Ala Ala Val Ala Phe Leu Leu Glu Ser Ile Thr Pro Val Gly Ser Val 
            180                 185                 190         


Leu Ala Leu Met Val Tyr Thr Ile Leu Phe Leu Lys Leu Phe Ser Tyr 
        195                 200                 205             


Arg Asp Val Asn Leu Trp Cys Arg Glu Arg Arg Ala Gly Ala Lys Ala 
    210                 215                 220                 


Lys Ala Ala Leu Ala Gly Lys Lys Ala Asn Gly Gly Ala Ala Gln Arg 
225                 230                 235                 240 


Thr Val Ser Tyr Pro Asp Asn Leu Thr Tyr Arg Asp Leu Tyr Tyr Phe 
                245                 250                 255     


Leu Phe Ala Pro Thr Leu Cys Tyr Glu Leu Asn Phe Pro Arg Ser Pro 
            260                 265                 270         


Arg Ile Arg Lys Arg Phe Leu Leu Arg Arg Leu Leu Glu Met Leu Phe 
        275                 280                 285             


Leu Thr Gln Leu Gln Val Gly Leu Ile Gln Gln Trp Met Val Pro Ala 
    290                 295                 300                 


Ile Gln Asn Ser Met Lys Pro Phe Lys Asp Met Asp Tyr Ser Arg Ile 
305                 310                 315                 320 


Val Glu Arg Leu Leu Lys Leu Ala Val Pro Asn His Leu Ile Trp Leu 
                325                 330                 335     


Ile Phe Phe Tyr Trp Leu Phe His Ser Cys Leu Asn Ala Val Ala Glu 
            340                 345                 350         


Leu Met Gln Phe Gly Asp Arg Glu Phe Tyr Arg Asp Trp Trp Asn Ser 
        355                 360                 365             


Glu Ser Ile Thr Tyr Phe Trp Gln Asn Trp Asn Ile Pro Val His Lys 
    370                 375                 380                 


Trp Cys Leu Arg His Phe Tyr Lys Pro Met Leu Arg Arg Gly Ser Ser 
385                 390                 395                 400 


Lys Trp Ala Ala Arg Thr Gly Val Phe Leu Ala Ser Ala Phe Phe His 
                405                 410                 415     


Glu Tyr Leu Val Ser Ile Pro Leu Arg Met Phe Arg Leu Trp Ala Phe 
            420                 425                 430         


Thr Gly Met Met Ala Gln Ile Pro Leu Ala Trp Ile Val Gly Arg Phe 
        435                 440                 445             


Phe Arg Gly Asn Tyr Gly Asn Ala Ala Val Trp Leu Ser Leu Ile Ile 
    450                 455                 460                 


Gly Gln Pro Val Ala Val Leu Met Tyr Val His Asp Tyr Tyr Val Leu 
465                 470                 475                 480 


Asn Arg Glu Ala Pro Thr Ala Gly Thr 
                485                 


<210>  4
<211>  1470
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of the protein coding region of Capra hircus 
       (goat) diacylglycerol O-acyltransferase 1 isoform X1 (ChDGAT1-X1)
       in pAT002, codon optimised for expression in yeast.

<400>  4
atgggtgata gaggtggtgc tggtggttct agaagaagaa ggacaggttc tagaccatct       60

attcaaggtg gttcaagacc agctgctgct gaagaagagg ttagagatgt tggtgccggt      120

ggtgatgctc cagttagaga tactgataag gatggtgatg ttgatgttgg ttctggtcat      180

tgggatttga gatgtcatag attgcaggac agcttgttct cttctgattc tggtttttct      240

aactacaggg gtattttgaa ctggtgcgtt gttatgttga ttttgtctaa cgctaggctg      300

ttcttggaga acttgattaa gtacggtatc ttggttgatc caatccaggt tgtttctttg      360

ttcttgaagg atccatattc ttggccagct ttgtgtttgg ttatcgttgc taacattttt      420

gctgttgctg ccttccaagt cgaaaaaaga ttggctgttg gtgctttgac tgaacaagca      480

ggtttgttgt tgcatggtgt taacttggct accattttgt gttttccagc tgcagttgct      540

ttcttgttgg aatctattac tccagtcggt tctgttttgg ctttgatggt ttacactatc      600

ctgttcctaa agctgttctc ctacagagat gttaatttgt ggtgtagaga aagacgtgct      660

ggtgctaaag ctaaagctgc tttggctggt aaaaaagcta atggtggtgc agctcaaaga      720

actgtttctt acccagataa cttgacttac agggacttgt actacttttt gttcgctcca      780

actttgtgct acgaattgaa ttttccaaga agcccaagga tcagaaagag gtttttgttg      840

agaaggttgt tggagatgtt gtttttgacc caattgcaag tcggtttgat ccaacaatgg      900

atggttccag ctattcagaa ttctatgaag ccattcaagg acatggacta ctctagaatc      960

gtcgaaagat tattgaagtt ggccgttcca aaccatttga tctggttgat ttttttctac     1020

tggctgttcc actcttgctt gaatgctgtt gcagaattga tgcaatttgg tgacagagaa     1080

ttttacagag actggtggaa ctctgaatcc attacttact tttggcagaa ctggaacatc     1140

ccagttcata agtggtgttt gagacatttc tacaagccaa tgttgcgtag aggttcttct     1200

aaatgggctg ctagaactgg tgtttttttg gcttctgctt tcttccacga atacttggtt     1260

tctatcccat tgagaatgtt cagattgtgg gcttttactg gtatgatggc tcaaattcca     1320

ttggcatgga tagttggtag attcttcaga ggtaattacg gtaatgctgc tgtttggttg     1380

tccttgatta ttggtcaacc agttgctgtt ttgatgtacg ttcatgatta ctacgtcttg     1440

aacagagaag ctccaactgc tggtacttaa                                      1470


<210>  5
<211>  482
<212>  PRT
<213>  Capra hircus

<400>  5

Met Gly Pro Leu Arg Pro Asn Leu Val Pro Gln Ala Tyr Arg Arg Leu 
1               5                   10                  15      


Leu Gly Leu Glu Ser Glu Ser Leu Ala Pro Gly Leu Cys Val His Ser 
            20                  25                  30          


Cys Ala Pro Leu Ala Ser Ala Gly Lys Leu Ser Gly Arg Leu Gly Ala 
        35                  40                  45              


Glu Gly Leu Leu Pro Gly Thr Pro Arg Cys His Arg Leu Gln Asp Ser 
    50                  55                  60                  


Leu Phe Ser Ser Asp Ser Gly Phe Ser Asn Tyr Arg Gly Ile Leu Asn 
65                  70                  75                  80  


Trp Cys Val Val Met Leu Ile Leu Ser Asn Ala Arg Leu Phe Leu Glu 
                85                  90                  95      


Asn Leu Ile Lys Tyr Gly Ile Leu Val Asp Pro Ile Gln Val Val Ser 
            100                 105                 110         


Leu Phe Leu Lys Asp Pro Tyr Ser Trp Pro Ala Leu Cys Leu Val Ile 
        115                 120                 125             


Val Ala Asn Ile Phe Ala Val Ala Ala Phe Gln Val Glu Lys Arg Leu 
    130                 135                 140                 


Ala Val Gly Ala Leu Thr Glu Gln Ala Gly Leu Leu Leu His Gly Val 
145                 150                 155                 160 


Asn Leu Ala Thr Ile Leu Cys Phe Pro Ala Ala Val Ala Phe Leu Leu 
                165                 170                 175     


Glu Ser Ile Thr Pro Val Gly Ser Val Leu Ala Leu Met Val Tyr Thr 
            180                 185                 190         


Ile Leu Phe Leu Lys Leu Phe Ser Tyr Arg Asp Val Asn Leu Trp Cys 
        195                 200                 205             


Arg Glu Arg Arg Ala Gly Ala Lys Ala Lys Ala Ala Leu Ala Gly Lys 
    210                 215                 220                 


Lys Ala Asn Gly Gly Ala Ala Gln Arg Thr Val Ser Tyr Pro Asp Asn 
225                 230                 235                 240 


Leu Thr Tyr Arg Asp Leu Tyr Tyr Phe Leu Phe Ala Pro Thr Leu Cys 
                245                 250                 255     


Tyr Glu Leu Asn Phe Pro Arg Ser Pro Arg Ile Arg Lys Arg Phe Leu 
            260                 265                 270         


Leu Arg Arg Leu Leu Glu Met Leu Phe Leu Thr Gln Leu Gln Val Gly 
        275                 280                 285             


Leu Ile Gln Gln Trp Met Val Pro Ala Ile Gln Asn Ser Met Lys Pro 
    290                 295                 300                 


Phe Lys Asp Met Asp Tyr Ser Arg Ile Val Glu Arg Leu Leu Lys Leu 
305                 310                 315                 320 


Ala Val Pro Asn His Leu Ile Trp Leu Ile Phe Phe Tyr Trp Leu Phe 
                325                 330                 335     


His Ser Cys Leu Asn Ala Val Ala Glu Leu Met Gln Phe Gly Asp Arg 
            340                 345                 350         


Glu Phe Tyr Arg Asp Trp Trp Asn Ser Glu Ser Ile Thr Tyr Phe Trp 
        355                 360                 365             


Gln Asn Trp Asn Ile Pro Val His Lys Trp Cys Leu Arg His Phe Tyr 
    370                 375                 380                 


Lys Pro Met Leu Arg Arg Gly Ser Ser Lys Trp Ala Ala Arg Thr Gly 
385                 390                 395                 400 


Val Phe Leu Ala Ser Ala Phe Phe His Glu Tyr Leu Val Ser Ile Pro 
                405                 410                 415     


Leu Arg Met Phe Arg Leu Trp Ala Phe Thr Gly Met Met Ala Gln Ile 
            420                 425                 430         


Pro Leu Ala Trp Ile Val Gly Arg Phe Phe Arg Gly Asn Tyr Gly Asn 
        435                 440                 445             


Ala Ala Val Trp Leu Ser Leu Ile Ile Gly Gln Pro Val Ala Val Leu 
    450                 455                 460                 


Met Tyr Val His Asp Tyr Tyr Val Leu Asn Arg Glu Ala Pro Thr Ala 
465                 470                 475                 480 


Gly Thr 
        


<210>  6
<211>  1449
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of the protein coding region of Capra hircus 
       (goat) diacylglycerol O-acyltransferase 1 isoform X2 (ChDGAT1-X2)
       in pAT003, codon optimised for expression in yeast.

<400>  6
atgggtcccc tgaggcctaa tttggttcca caagcctata gaagattgct aggtttggaa       60

tctgaatctt tggctccagg tttgtgtgtt cattcttgtg ctccattggc ttcagctggt      120

aaattgtctg gtagattggg tgctgaaggt ttgttgccag gtactccaag atgtcataga      180

ttgcaagatt ccttgttctc ctctgattct ggtttctcta actacagagg tattttgaac      240

tggtgcgttg tcatgttgat tttgtctaat gctaggctgt tcttggagaa cttgattaag      300

tacggtatct tggttgatcc aatccaggtt gtttctttgt tcttgaagga tccatattct      360

tggccagctt tgtgtttggt tatcgttgct aacatttttg ctgttgctgc cttccaagtc      420

gaaaaaagat tggctgttgg tgctttgact gaacaagctg gtttgttgtt gcatggtgtt      480

aacttggcta ccattttgtg ttttccagct gctgttgcat ttctgttgga atctattact      540

ccagttggtt ccgttttggc tttgatggtt tacactatcc tgttcctaaa gctgttctcc      600

tacagagatg ttaatttgtg gtgtagagaa agacgtgctg gtgctaaagc taaagctgct      660

ttggctggta aaaaagctaa tggtggtgct gctcaaagaa ctgtttctta cccagataac      720

ttgacttaca gggacttgta ctactttttg ttcgctccaa ctttgtgcta cgaattgaat      780

tttccaagaa gcccaagaat ccgtaagagg tttttgttga gaaggttgtt ggagatgttg      840

tttttgaccc aattgcaagt cggtttgatc caacaatgga tggttccagc tattcagaat      900

tctatgaagc cattcaagga catggactac tctagaatcg tcgaaagatt attgaagttg      960

gccgttccaa accatttgat ctggttgatt tttttctact ggctgttcca ctcttgcttg     1020

aatgcagttg ctgaattgat gcaatttggt gacagagaat tttacagaga ctggtggaac     1080

tccgaatcca ttacttactt ttggcaaaac tggaacatcc cagttcataa gtggtgtttg     1140

agacatttct acaagccaat gcttagaagg ggttcttcta aatgggctgc tagaactggt     1200

gtttttttgg cttctgcttt cttccacgaa tacttggttt ctatcccatt gagaatgttc     1260

agattgtggg cttttactgg tatgatggct caaattccat tggcatggat agttggtaga     1320

ttcttcagag gtaattacgg taatgctgct gtttggttgt ccttgattat tggtcaacca     1380

gttgctgttt tgatgtacgt tcatgattac tacgtcttga acagagaagc tccaactgct     1440

ggtacttaa                                                             1449


<210>  7
<211>  489
<212>  PRT
<213>  Ovis aries

<400>  7

Met Gly Asp Arg Gly Gly Ala Gly Gly Ser Arg Arg Arg Arg Thr Gly 
1               5                   10                  15      


Ser Arg Pro Ser Ile Gln Gly Gly Ser Arg Pro Ala Ala Ala Glu Glu 
            20                  25                  30          


Glu Val Arg Asp Val Gly Ala Gly Gly Asp Ala Pro Val Arg Asp Thr 
        35                  40                  45              


Asp Lys Asp Gly Asp Val Asp Val Gly Ser Gly His Trp Asp Leu Arg 
    50                  55                  60                  


Cys His Arg Leu Gln Asp Ser Leu Phe Ser Ser Asp Ser Gly Phe Ser 
65                  70                  75                  80  


Asn Tyr Arg Gly Ile Leu Asn Trp Cys Val Val Met Leu Ile Leu Ser 
                85                  90                  95      


Asn Ala Arg Leu Phe Leu Glu Asn Leu Ile Lys Tyr Gly Ile Leu Val 
            100                 105                 110         


Asp Pro Ile Gln Val Val Ser Leu Phe Leu Lys Asp Pro Tyr Ser Trp 
        115                 120                 125             


Pro Ala Leu Cys Leu Val Ile Val Ala Asn Ile Phe Ala Val Ala Ala 
    130                 135                 140                 


Phe Gln Val Glu Lys Arg Leu Ala Val Gly Ala Leu Thr Glu Gln Ala 
145                 150                 155                 160 


Gly Leu Leu Leu His Gly Val Asn Leu Ala Thr Ile Leu Cys Phe Pro 
                165                 170                 175     


Ala Ala Val Ala Phe Leu Leu Glu Ser Ile Thr Pro Val Gly Ser Val 
            180                 185                 190         


Leu Ala Leu Met Val Tyr Thr Ile Leu Phe Leu Lys Leu Phe Ser Tyr 
        195                 200                 205             


Arg Asp Val Asn Leu Trp Cys Arg Glu Arg Arg Ala Gly Ala Lys Ala 
    210                 215                 220                 


Lys Ala Ala Leu Ala Gly Lys Lys Ala Asn Gly Gly Ala Ala Gln Arg 
225                 230                 235                 240 


Thr Val Ser Tyr Pro Asp Asn Leu Thr Tyr Arg Asp Leu Tyr Tyr Phe 
                245                 250                 255     


Leu Phe Ala Pro Thr Leu Cys Tyr Glu Leu Asn Phe Pro Arg Ser Pro 
            260                 265                 270         


Arg Ile Arg Lys Arg Phe Leu Leu Arg Arg Leu Leu Glu Met Leu Phe 
        275                 280                 285             


Leu Thr Gln Leu Gln Val Gly Leu Ile Gln Gln Trp Met Val Pro Ala 
    290                 295                 300                 


Ile Gln Asn Ser Met Lys Pro Phe Lys Asp Met Asp Tyr Ser Arg Ile 
305                 310                 315                 320 


Val Glu Arg Leu Leu Lys Leu Ala Val Pro Asn His Leu Ile Trp Leu 
                325                 330                 335     


Ile Phe Phe Tyr Trp Leu Phe His Ser Cys Leu Asn Ala Val Ala Glu 
            340                 345                 350         


Leu Met Gln Phe Gly Asp Arg Glu Phe Tyr Arg Asp Trp Trp Asn Ser 
        355                 360                 365             


Glu Ser Ile Thr Tyr Phe Trp Gln Asn Trp Asn Ile Pro Val His Lys 
    370                 375                 380                 


Trp Cys Leu Arg His Phe Tyr Lys Pro Met Leu Arg Arg Gly Ser Ser 
385                 390                 395                 400 


Lys Trp Ala Ala Arg Thr Gly Val Phe Leu Ala Ser Ala Phe Phe His 
                405                 410                 415     


Glu Tyr Leu Val Ser Ile Pro Leu Arg Met Phe Arg Leu Trp Ala Phe 
            420                 425                 430         


Thr Gly Met Met Ala Gln Ile Pro Leu Ala Trp Ile Val Gly Arg Phe 
        435                 440                 445             


Phe Arg Gly Asn Tyr Gly Asn Ala Ala Val Trp Leu Ser Leu Ile Ile 
    450                 455                 460                 


Gly Gln Pro Val Ala Val Leu Met Tyr Val His Asp Tyr Tyr Val Leu 
465                 470                 475                 480 


Asn Arg Glu Ala Pro Thr Ala Gly Thr 
                485                 


<210>  8
<211>  1470
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of the protein coding region of Ovis aries 
       (sheep) diacylglycerol O-acyltransferase 1 isoform X1 
       (OaDGAT1-X1) in pAT004, codon optimised for expression in yeast.

<400>  8
atgggtgata gaggtggtgc tggtggttct agaagaagaa ggacaggttc tagaccatct       60

attcaaggtg gttcaagacc agctgctgct gaagaagagg ttagagatgt tggtgccggt      120

ggtgatgctc cagttagaga tactgataag gatggtgatg ttgatgttgg ttctggtcat      180

tgggatttga gatgtcatag attgcaggac agcttgttct cttctgattc tggtttttct      240

aactacaggg gtattttgaa ctggtgcgtt gttatgttga ttttgtctaa cgctaggctg      300

ttcttggaga acttgattaa gtacggtatc ttggttgatc caatccaggt tgtttctttg      360

ttcttgaagg atccatattc ttggccagct ttgtgtttgg ttatcgttgc taacattttt      420

gctgttgctg ccttccaagt cgaaaaaaga ttggctgttg gtgctttgac tgaacaagca      480

ggtttgttgt tgcatggtgt taacttggct accattttgt gttttccagc tgcagttgct      540

ttcttgttgg aatctattac tccagtcggt tctgttttgg ctttgatggt ttacactatc      600

ctgttcctaa agctgttctc ctacagagat gttaatttgt ggtgtagaga aagacgtgct      660

ggtgctaaag ctaaagctgc tttggctggt aaaaaagcta atggtggtgc agctcaaaga      720

actgtttctt acccagataa cttgacttac agggacttgt actacttttt gttcgctcca      780

actttgtgct acgaattgaa ttttccaaga agcccaagga tcagaaagag gtttttgttg      840

agaaggttgt tggagatgtt gtttttgacc caattgcaag tcggtttgat ccaacaatgg      900

atggttccag ctattcagaa ttctatgaag ccattcaagg acatggacta ctctagaatc      960

gtcgaaagat tattgaagtt ggccgttcca aaccatttga tctggttgat ttttttctac     1020

tggctgttcc actcttgctt gaatgctgtt gcagaattga tgcaatttgg tgacagagaa     1080

ttttacagag actggtggaa ctctgaatcc attacttact tttggcagaa ctggaacatc     1140

ccagttcata agtggtgttt gagacatttc tacaagccaa tgttgcgtag aggttcttct     1200

aaatgggctg ctagaactgg tgtttttttg gcttctgctt tcttccacga atacttggtt     1260

tctatcccat tgagaatgtt cagattgtgg gcttttactg gtatgatggc tcaaattcca     1320

ttggcatgga tagttggtag attcttcaga ggtaattacg gtaatgctgc tgtttggttg     1380

tccttgatta ttggtcaacc agttgctgtt ttgatgtacg ttcatgatta ctacgtcttg     1440

aacagagaag ctccaactgc tggtacttaa                                      1470


<210>  9
<211>  467
<212>  PRT
<213>  Ovis aries

<400>  9

Met Gly Asp Arg Gly Gly Ala Gly Gly Ser Arg Arg Arg Arg Thr Gly 
1               5                   10                  15      


Ser Arg Pro Ser Ile Gln Gly Gly Ser Arg Pro Ala Ala Ala Glu Glu 
            20                  25                  30          


Glu Val Arg Asp Val Gly Ala Gly Gly Asp Ala Pro Val Arg Asp Thr 
        35                  40                  45              


Asp Lys Asp Gly Asp Val Asp Val Gly Ser Gly His Trp Asp Leu Arg 
    50                  55                  60                  


Cys His Arg Leu Gln Asp Ser Leu Phe Ser Ser Asp Ser Gly Phe Ser 
65                  70                  75                  80  


Asn Tyr Arg Gly Ile Leu Asn Trp Cys Val Val Met Leu Ile Leu Ser 
                85                  90                  95      


Asn Ala Arg Leu Phe Leu Glu Asn Leu Ile Lys Tyr Gly Ile Leu Val 
            100                 105                 110         


Asp Pro Ile Gln Val Val Ser Leu Phe Leu Lys Asp Pro Tyr Ser Trp 
        115                 120                 125             


Pro Ala Leu Cys Leu Val Ile Val Ala Asn Ile Phe Ala Val Ala Ala 
    130                 135                 140                 


Phe Gln Val Glu Lys Arg Leu Ala Val Gly Ala Leu Thr Glu Gln Ala 
145                 150                 155                 160 


Gly Leu Leu Leu His Gly Val Asn Leu Ala Thr Ile Leu Cys Phe Pro 
                165                 170                 175     


Ala Ala Val Ala Phe Leu Leu Glu Ser Ile Thr Pro Val Gly Ser Val 
            180                 185                 190         


Leu Ala Leu Met Val Tyr Thr Ile Leu Phe Leu Lys Leu Phe Ser Tyr 
        195                 200                 205             


Arg Asp Val Asn Leu Trp Cys Arg Glu Arg Arg Ala Gly Ala Lys Ala 
    210                 215                 220                 


Lys Ala Ala Leu Ala Asp Leu Tyr Tyr Phe Leu Phe Ala Pro Thr Leu 
225                 230                 235                 240 


Cys Tyr Glu Leu Asn Phe Pro Arg Ser Pro Arg Ile Arg Lys Arg Phe 
                245                 250                 255     


Leu Leu Arg Arg Leu Leu Glu Met Leu Phe Leu Thr Gln Leu Gln Val 
            260                 265                 270         


Gly Leu Ile Gln Gln Trp Met Val Pro Ala Ile Gln Asn Ser Met Lys 
        275                 280                 285             


Pro Phe Lys Asp Met Asp Tyr Ser Arg Ile Val Glu Arg Leu Leu Lys 
    290                 295                 300                 


Leu Ala Val Pro Asn His Leu Ile Trp Leu Ile Phe Phe Tyr Trp Leu 
305                 310                 315                 320 


Phe His Ser Cys Leu Asn Ala Val Ala Glu Leu Met Gln Phe Gly Asp 
                325                 330                 335     


Arg Glu Phe Tyr Arg Asp Trp Trp Asn Ser Glu Ser Ile Thr Tyr Phe 
            340                 345                 350         


Trp Gln Asn Trp Asn Ile Pro Val His Lys Trp Cys Leu Arg His Phe 
        355                 360                 365             


Tyr Lys Pro Met Leu Arg Arg Gly Ser Ser Lys Trp Ala Ala Arg Thr 
    370                 375                 380                 


Gly Val Phe Leu Ala Ser Ala Phe Phe His Glu Tyr Leu Val Ser Ile 
385                 390                 395                 400 


Pro Leu Arg Met Phe Arg Leu Trp Ala Phe Thr Gly Met Met Ala Gln 
                405                 410                 415     


Ile Pro Leu Ala Trp Ile Val Gly Arg Phe Phe Arg Gly Asn Tyr Gly 
            420                 425                 430         


Asn Ala Ala Val Trp Leu Ser Leu Ile Ile Gly Gln Pro Val Ala Val 
        435                 440                 445             


Leu Met Tyr Val His Asp Tyr Tyr Val Leu Asn Arg Glu Ala Pro Thr 
    450                 455                 460                 


Ala Gly Thr 
465         


<210>  10
<211>  1404
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of the protein coding region of Ovis aries 
       (sheep) diacylglycerol O-acyltransferase 1 isoform X2 
       (OaDGAT1-X2) in pAT005, codon optimised for expression in yeast.

<400>  10
atgggtgata gaggtggtgc tggtggttct agaagaagaa ggacaggttc tagaccatct       60

attcaaggtg gttcaagacc agctgctgct gaagaagagg ttagagatgt tggtgccggt      120

ggtgatgctc cagttagaga tactgataag gatggtgatg ttgatgttgg ttctggtcat      180

tgggatttga gatgtcatag attgcaggac agcttgttct cttctgattc tggtttttct      240

aactacaggg gtattttgaa ctggtgcgtt gttatgttga ttttgtctaa cgctaggctg      300

ttcttggaga acttgattaa gtacggtatc ttggttgatc caatccaggt tgtttctttg      360

ttcttgaagg atccatattc ttggccagct ttgtgtttgg ttatcgttgc taacattttt      420

gctgttgctg ccttccaagt cgaaaaaaga ttggctgttg gtgctttgac tgaacaagca      480

ggtttgttgt tgcatggtgt taacttggct accattttgt gttttccagc tgcagttgct      540

ttcttgttgg aatctattac tccagtcggt tctgttttgg ctttgatggt ttacactatc      600

ctgttcctaa agctgttctc ctacagagat gttaatttgt ggtgtagaga aagacgtgct      660

ggtgctaaag ctaaagctgc tttggctgac ttgtactact ttttgtttgc tccaaccttg      720

tgctacgaat tgaattttcc aagatctccc aggatcagaa agaggttttt gttgagaaga      780

ttgctggaaa tgctgttttt gacccaattg caagtcggtt tgattcaaca atggatggtt      840

ccagctatcc agaattctat gaagccattc aaggatatgg actactccag aatcgtcgaa      900

aggttgttga aattggcagt tccaaaccat ttgatctggc tgattttttt ctactggttg      960

ttccactctt gcttgaatgc tgttgcagaa ttgatgcaat ttggtgacag agaattttac     1020

agagactggt ggaactctga atccattact tacttttggc agaactggaa catcccagtt     1080

cataagtggt gtttgagaca tttctacaag ccaatgttgc gtagaggttc ttctaaatgg     1140

gctgctagaa ctggtgtttt tttggcttct gctttcttcc acgaatactt ggtttctatc     1200

ccattgagaa tgttcagatt gtgggctttt actggtatga tggctcaaat tccattagct     1260

tggatcgttg gtagattctt cagaggtaat tatggtaatg ctgccgtttg gttgtccttg     1320

attattggtc aaccagttgc tgttttgatg tacgttcatg attactacgt cttgaacaga     1380

gaagctccaa ctgctggtac ttaa                                            1404


<210>  11
<211>  566
<212>  PRT
<213>  Bos taurus

<400>  11

Met Gly Asp Arg Gly Gly Ala Gly Gly Ser Arg Arg Arg Arg Thr Gly 
1               5                   10                  15      


Ser Arg Pro Ser Ile Gln Gly Gly Ser Gly Pro Ala Ala Ala Glu Glu 
            20                  25                  30          


Glu Val Arg Asp Val Gly Ala Gly Gly Asp Ala Pro Val Arg Asp Thr 
        35                  40                  45              


Asp Lys Asp Gly Asp Val Asp Val Gly Ser Gly His Trp Asp Leu Arg 
    50                  55                  60                  


Cys His Arg Leu Gln Asp Ser Leu Phe Ser Ser Asp Ser Gly Phe Ser 
65                  70                  75                  80  


Asn Tyr Arg Gly Ile Leu Asn Trp Cys Val Val Met Leu Ile Leu Ser 
                85                  90                  95      


Asn Ala Arg Leu Phe Leu Glu Asn Leu Ile Lys Tyr Gly Ile Leu Val 
            100                 105                 110         


Asp Pro Ile Gln Val Val Ser Leu Phe Leu Lys Asp Pro Tyr Ser Trp 
        115                 120                 125             


Pro Ala Leu Cys Leu Val Ile Val Ala Asn Ile Phe Ala Val Ala Ala 
    130                 135                 140                 


Phe Gln Val Glu Lys Arg Leu Ala Val Gly Ala Leu Thr Glu Gln Ala 
145                 150                 155                 160 


Gly Leu Leu Leu His Gly Val Asn Leu Ala Thr Ile Leu Cys Phe Pro 
                165                 170                 175     


Ala Ala Val Ala Phe Leu Leu Glu Ser Ile Thr Pro Val Gly Ser Val 
            180                 185                 190         


Leu Ala Leu Met Val Tyr Thr Ile Leu Phe Leu Lys Leu Phe Ser Tyr 
        195                 200                 205             


Arg Asp Val Asn Leu Trp Cys Arg Glu Arg Arg Ala Gly Ala Lys Ala 
    210                 215                 220                 


Lys Ala Ala Leu Ala Gly Lys Ala Ala Asn Gly Gly Ala Ala Gln Arg 
225                 230                 235                 240 


Thr Val Ser Tyr Pro Asp Asn Leu Thr Tyr Arg Gly Glu Asp Pro Ala 
                245                 250                 255     


Gly Gly Trp Gly Asp Cys Pro Ala Ala Trp Pro Ala Ser Pro Ala Leu 
            260                 265                 270         


Pro Ser Arg Ser Leu Leu Leu Pro Leu Arg Pro His Pro Val Leu Arg 
        275                 280                 285             


Ala Gln Leu Pro Pro Leu Pro Pro His Pro Lys Ala Leu Pro Ala Ala 
    290                 295                 300                 


Ala Thr Pro Gly Asp Ala Val Pro His Pro Ala Pro Gly Gly Ala Asp 
305                 310                 315                 320 


Pro Ala Val Asp Gly Pro Gly His Pro Glu Leu His Glu Ala Leu Gln 
                325                 330                 335     


Gly His Gly Leu Leu Pro His Arg Gly Ala Pro Pro Glu Ala Gly Gly 
            340                 345                 350         


Glu Trp Pro Ala Gly Trp Gly Arg Val Gly Ala Gly Gly Ala Val Leu 
        355                 360                 365             


Ala Pro Gly Thr His Ser Pro Gln Val Pro Asn His Leu Ile Trp Leu 
    370                 375                 380                 


Ile Phe Phe Tyr Trp Leu Phe His Ser Cys Leu Asn Ala Val Ala Glu 
385                 390                 395                 400 


Leu Met Gln Phe Gly Asp Arg Glu Phe Tyr Arg Asp Trp Trp Trp Val 
                405                 410                 415     


Ala Leu Pro Gly Arg Gly Trp Trp Gly Pro Pro Leu Gly Leu Gly Pro 
            420                 425                 430         


Glu Pro Leu Pro Thr Leu Pro Arg Pro Arg Arg Asn Ser Glu Ser Ile 
        435                 440                 445             


Thr Tyr Phe Trp Gln Asn Trp Asn Ile Pro Val His Lys Trp Cys Ile 
    450                 455                 460                 


Arg His Phe Tyr Lys Pro Met Leu Arg Arg Gly Ser Ser Lys Trp Ala 
465                 470                 475                 480 


Ala Arg Thr Ala Val Phe Leu Ala Ser Ala Phe Phe His Glu Tyr Leu 
                485                 490                 495     


Val Ser Ile Pro Leu Arg Met Phe Arg Leu Trp Ala Phe Thr Gly Met 
            500                 505                 510         


Met Ala Gln Ile Pro Leu Ala Trp Ile Val Gly Arg Phe Phe Arg Gly 
        515                 520                 525             


Asn Tyr Gly Asn Ala Ala Val Trp Leu Ser Leu Ile Ile Gly Gln Pro 
    530                 535                 540                 


Val Ala Val Leu Met Tyr Val His Asp Tyr Tyr Val Leu Asn Arg Glu 
545                 550                 555                 560 


Ala Pro Ala Ala Gly Thr 
                565     


<210>  12
<211>  1701
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of the protein coding region of Bos taurus 
       (cattle) diacylglycerol O-acyltransferase 1 isoform X1 
       (BtDGAT1-X1) in pAT006, codon optimised for expression in yeast.

<400>  12
atgggtgata gaggtggtgc tggtggttct agaagaagaa ggacaggttc tagaccatct       60

attcaaggtg gttctggtcc agctgctgct gaagaagagg ttagagatgt tggtgccggt      120

ggtgatgctc cagttagaga tactgataag gatggtgatg ttgatgttgg ttcaggtcat      180

tgggatttga gatgtcatag attgcaggac agcttgttct cttctgattc tggtttttct      240

aactacaggg gtattttgaa ctggtgcgtt gttatgttga ttttgtctaa cgctaggctg      300

ttcttggaga acttgattaa gtacggtatc ttggttgatc caatccaggt tgtttctttg      360

ttcttgaagg atccatattc ttggccagct ttgtgtttgg ttatcgttgc taacattttt      420

gctgttgctg ccttccaagt cgaaaaaaga ttggctgttg gtgctttgac tgaacaagca      480

ggtttgttgt tgcatggtgt taacttggct accattttgt gttttccagc tgcagttgct      540

ttcttgttgg aatctattac tccagttggt tccgttttgg ctttgatggt ttacactatc      600

ctgttcctaa agctgttctc ctacagagat gttaatttgt ggtgtagaga aagacgtgct      660

ggtgctaaag ctaaagctgc tttggctggt aaagctgcaa atggtggtgc agctcaaaga      720

actgtttctt acccagataa cttgacctac agaggtgaag atccagccgg tggttggggt      780

gattgtccag cagcttggcc tgcttctcca gctttgccat ctagatcttt gttgttacca      840

ttaagaccac atccagtttt gagagcacaa ttgccaccat tgcctccaca tccaaaggct      900

ttgcctgctg ctgctactcc aggtgatgct gttccacatc ctgctccagg tggtgccgat      960

ccagctgttg atggtccagg tcatccagaa ttgcatgaag ccttacaagg tcatggtttg     1020

ttgccacata gaggtgctcc acctgaagct ggtggtgaat ggccagcagg ttggggtaga     1080

gttggtgcag gcggtgctgt tttggctcca ggtactcatt ctccacaagt tccaaaccat     1140

ttgatctggc tgattttctt ctactggttg ttccattctt gcttgaatgc tgttgcagaa     1200

ttgatgcaat ttggtgacag agaattttac agagattggt ggtgggttgc tttgccaggt     1260

agaggatggt ggggtccacc attaggtttg ggtccagaac cattgccaac tttgccaaga     1320

ccaagaagaa actctgaatc cattacctac ttttggcaga actggaacat tcctgttcat     1380

aagtggtgca tcagacattt ctataagcca atgttgagaa ggggttcttc taaatgggct     1440

gctagaactg ctgttttttt ggcttctgct ttcttccacg aatacttggt ttctatccca     1500

ttgagaatgt tcagattgtg ggcttttact ggtatgatgg ctcaaattcc attggcttgg     1560

atagttggta gattcttcag aggtaattac ggtaatgctg ctgtttggtt gtccttgatt     1620

attggtcaac cagttgcagt tttgatgtac gttcatgatt actacgtctt gaacagagaa     1680

gctccagctg ccggtactta a                                               1701


<210>  13
<211>  467
<212>  PRT
<213>  Bos taurus

<400>  13

Met Gly Asp Arg Gly Gly Ala Gly Gly Ser Arg Arg Arg Arg Thr Gly 
1               5                   10                  15      


Ser Arg Pro Ser Ile Gln Gly Gly Ser Gly Pro Ala Ala Ala Glu Glu 
            20                  25                  30          


Glu Val Arg Asp Val Gly Ala Gly Gly Asp Ala Pro Val Arg Asp Thr 
        35                  40                  45              


Asp Lys Asp Gly Asp Val Asp Val Gly Ser Gly His Trp Asp Leu Arg 
    50                  55                  60                  


Cys His Arg Leu Gln Asp Ser Leu Phe Ser Ser Asp Ser Gly Phe Ser 
65                  70                  75                  80  


Asn Tyr Arg Gly Ile Leu Asn Trp Cys Val Val Met Leu Ile Leu Ser 
                85                  90                  95      


Asn Ala Arg Leu Phe Leu Glu Asn Leu Ile Lys Tyr Gly Ile Leu Val 
            100                 105                 110         


Asp Pro Ile Gln Val Val Ser Leu Phe Leu Lys Asp Pro Tyr Ser Trp 
        115                 120                 125             


Pro Ala Leu Cys Leu Val Ile Val Ala Asn Ile Phe Ala Val Ala Ala 
    130                 135                 140                 


Phe Gln Val Glu Lys Arg Leu Ala Val Gly Ala Leu Thr Glu Gln Ala 
145                 150                 155                 160 


Gly Leu Leu Leu His Gly Val Asn Leu Ala Thr Ile Leu Cys Phe Pro 
                165                 170                 175     


Ala Ala Val Ala Phe Leu Leu Glu Ser Ile Thr Pro Val Gly Ser Val 
            180                 185                 190         


Leu Ala Leu Met Val Tyr Thr Ile Leu Phe Leu Lys Leu Phe Ser Tyr 
        195                 200                 205             


Arg Asp Val Asn Leu Trp Cys Arg Glu Arg Arg Ala Gly Ala Lys Ala 
    210                 215                 220                 


Lys Ala Ala Leu Ala Asp Leu Tyr Tyr Phe Leu Phe Ala Pro Thr Leu 
225                 230                 235                 240 


Cys Tyr Glu Leu Asn Phe Pro Arg Ser Pro Arg Ile Arg Lys Arg Phe 
                245                 250                 255     


Leu Leu Arg Arg Leu Leu Glu Met Leu Phe Leu Thr Gln Leu Gln Val 
            260                 265                 270         


Gly Leu Ile Gln Gln Trp Met Val Pro Ala Ile Gln Asn Ser Met Lys 
        275                 280                 285             


Pro Phe Lys Asp Met Asp Tyr Ser Arg Ile Val Glu Arg Leu Leu Lys 
    290                 295                 300                 


Leu Ala Val Pro Asn His Leu Ile Trp Leu Ile Phe Phe Tyr Trp Leu 
305                 310                 315                 320 


Phe His Ser Cys Leu Asn Ala Val Ala Glu Leu Met Gln Phe Gly Asp 
                325                 330                 335     


Arg Glu Phe Tyr Arg Asp Trp Trp Asn Ser Glu Ser Ile Thr Tyr Phe 
            340                 345                 350         


Trp Gln Asn Trp Asn Ile Pro Val His Lys Trp Cys Ile Arg His Phe 
        355                 360                 365             


Tyr Lys Pro Met Leu Arg Arg Gly Ser Ser Lys Trp Ala Ala Arg Thr 
    370                 375                 380                 


Ala Val Phe Leu Ala Ser Ala Phe Phe His Glu Tyr Leu Val Ser Ile 
385                 390                 395                 400 


Pro Leu Arg Met Phe Arg Leu Trp Ala Phe Thr Gly Met Met Ala Gln 
                405                 410                 415     


Ile Pro Leu Ala Trp Ile Val Gly Arg Phe Phe Arg Gly Asn Tyr Gly 
            420                 425                 430         


Asn Ala Ala Val Trp Leu Ser Leu Ile Ile Gly Gln Pro Val Ala Val 
        435                 440                 445             


Leu Met Tyr Val His Asp Tyr Tyr Val Leu Asn Arg Glu Ala Pro Ala 
    450                 455                 460                 


Ala Gly Thr 
465         


<210>  14
<211>  1404
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of the protein coding region of Bos taurus 
       (cattle) diacylglycerol O-acyltransferase 1 isoform X2 
       (BtDGAT1-X2) in pAT007, codon optimised for expression in yeast.

<400>  14
atgggtgata gaggtggtgc tggtggttct agaagaagaa ggacaggttc tagaccatct       60

attcaaggtg gttctggtcc agctgctgct gaagaagagg ttagagatgt tggtgccggt      120

ggtgatgctc cagttagaga tactgataag gatggtgatg ttgatgttgg ttcaggtcat      180

tgggatttga gatgtcatag attgcaggac agcttgttct cttctgattc tggtttttct      240

aactacaggg gtattttgaa ctggtgcgtt gttatgttga ttttgtctaa cgctaggctg      300

ttcttggaga acttgattaa gtacggtatc ttggttgatc caatccaggt tgtttctttg      360

ttcttgaagg atccatattc ttggccagct ttgtgtttgg ttatcgttgc taacattttt      420

gctgttgctg ccttccaagt cgaaaaaaga ttggctgttg gtgctttgac tgaacaagca      480

ggtttgttgt tgcatggtgt taacttggct accattttgt gttttccagc tgcagttgct      540

ttcttgttgg aatctattac tccagttggt tccgttttgg ctttgatggt ttacactatc      600

ctgttcctaa agctgttctc ctacagagat gttaatttgt ggtgtagaga aagacgtgct      660

ggtgctaaag ctaaagctgc tttggctgac ttgtactact ttttgtttgc tccaaccttg      720

tgctacgaat tgaattttcc aagatctccc aggatcagaa agaggttttt gttgagaaga      780

ttgctggaaa tgctgttttt gacccaattg caagtcggtt tgattcaaca atggatggtt      840

ccagctatcc agaattctat gaagccattc aaggatatgg actactccag aatcgtcgaa      900

aggttgttga aattggcagt tccaaaccat ttgatctggc tgattttttt ctactggttg      960

ttccactctt gcttgaatgc tgttgcagaa ttgatgcaat ttggtgacag agaattttac     1020

agagactggt ggaactctga atccattact tacttttggc agaactggaa catcccagtt     1080

cataagtggt gtatcagaca tttctacaag ccaatgttgc gtagaggttc ttctaaatgg     1140

gctgctagaa cagcagtttt tttggcttct gctttcttcc acgaatactt ggtttctatc     1200

ccattgagaa tgttcagatt gtgggctttt actggtatga tggctcaaat tccattagct     1260

tggatcgttg gtagattctt cagaggtaat tatggtaatg ctgccgtttg gttgtccttg     1320

attattggtc aaccagttgc tgttttgatg tacgttcatg attactacgt cttgaacaga     1380

gaagctcctg ctgctggtac ttaa                                            1404


<210>  15
<211>  519
<212>  PRT
<213>  Bubalus bubalis

<400>  15

Met Cys Pro Gln Glu Gly Lys Gly Ser Pro Gly Leu Ser Pro Tyr Lys 
1               5                   10                  15      


Thr Gly Gly Leu Ala Gln Cys Pro Gly Trp Pro Pro Gly Gly Arg Val 
            20                  25                  30          


Val Gly Gly Arg Trp Gly Cys Cys Leu Glu Pro Ala Ser Gly Ala Gly 
        35                  40                  45              


Pro Gly Leu Ser Cys Ala Pro Pro Pro Leu Pro Pro Ala Glu Gly Trp 
    50                  55                  60                  


Leu Pro Ser Pro Pro Asp Pro Ala Leu Gly Ala Gly Leu Ser Glu Val 
65                  70                  75                  80  


Gly Ser Glu Gly Pro Leu Leu Ala Ser Ala Val Asp Ser Arg Cys His 
                85                  90                  95      


Arg Leu Gln Asp Ser Leu Phe Ser Ser Asp Ser Gly Phe Ser Asn Tyr 
            100                 105                 110         


Arg Gly Ile Leu Asn Trp Cys Val Val Met Leu Ile Leu Ser Asn Ala 
        115                 120                 125             


Arg Leu Phe Leu Glu Asn Leu Ile Lys Tyr Gly Ile Leu Val Asp Pro 
    130                 135                 140                 


Ile Gln Val Val Ser Leu Phe Leu Lys Asp Pro Tyr Ser Trp Pro Ala 
145                 150                 155                 160 


Leu Cys Leu Val Ile Val Ala Asn Ile Phe Ala Val Ala Ala Phe Gln 
                165                 170                 175     


Val Glu Lys Arg Leu Ala Val Gly Ala Leu Thr Glu Gln Ala Gly Leu 
            180                 185                 190         


Leu Leu His Gly Val Asn Leu Ala Thr Ile Leu Cys Phe Pro Ala Ala 
        195                 200                 205             


Val Ala Phe Leu Leu Glu Ser Ile Thr Pro Val Gly Ser Val Leu Ala 
    210                 215                 220                 


Leu Met Val Tyr Thr Ile Leu Phe Leu Lys Leu Phe Ser Tyr Arg Asp 
225                 230                 235                 240 


Val Asn Leu Trp Cys Arg Glu Arg Arg Ala Gly Ala Lys Ala Lys Ala 
                245                 250                 255     


Ala Leu Ala Gly Lys Lys Ala Asn Gly Gly Ala Ala Gln Arg Thr Val 
            260                 265                 270         


Ser Tyr Pro Asp Asn Leu Thr Tyr Arg Asp Leu Tyr Tyr Phe Leu Phe 
        275                 280                 285             


Ala Pro Thr Leu Cys Tyr Glu Leu Asn Phe Pro Arg Ser Pro Arg Ile 
    290                 295                 300                 


Arg Lys Arg Phe Leu Leu Arg Arg Leu Leu Glu Met Leu Phe Leu Thr 
305                 310                 315                 320 


Gln Leu Gln Val Gly Leu Ile Gln Gln Trp Met Val Pro Ala Ile Gln 
                325                 330                 335     


Asn Ser Met Lys Pro Phe Lys Asp Met Asp Tyr Ser Arg Ile Val Glu 
            340                 345                 350         


Arg Leu Leu Lys Leu Ala Val Pro Asn His Leu Ile Trp Leu Ile Phe 
        355                 360                 365             


Phe Tyr Trp Leu Phe His Ser Cys Leu Asn Ala Val Ala Glu Leu Met 
    370                 375                 380                 


Gln Phe Gly Asp Arg Glu Phe Tyr Arg Asp Trp Trp Asn Ser Glu Ser 
385                 390                 395                 400 


Ile Thr Tyr Phe Trp Gln Asn Trp Asn Ile Pro Val His Lys Trp Cys 
                405                 410                 415     


Ile Arg His Phe Tyr Lys Pro Met Leu Arg Arg Gly Ser Ser Lys Trp 
            420                 425                 430         


Ala Ala Arg Thr Ala Val Phe Leu Ala Ser Ala Phe Phe His Glu Tyr 
        435                 440                 445             


Leu Val Ser Ile Pro Leu Arg Met Phe Arg Leu Trp Ala Phe Thr Gly 
    450                 455                 460                 


Met Met Ala Gln Ile Pro Leu Ala Trp Ile Val Gly Arg Phe Phe Arg 
465                 470                 475                 480 


Gly Asn Tyr Gly Asn Ala Ala Val Trp Leu Ser Leu Ile Ile Gly Gln 
                485                 490                 495     


Pro Val Ala Val Leu Met Tyr Val His Asp Tyr Tyr Val Leu Asn Arg 
            500                 505                 510         


Glu Ala Pro Ala Ala Gly Thr 
        515                 


<210>  16
<211>  1560
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of the protein coding region of Bubulus 
       bubulis (water buffalo) diacylglycerol O-acyltransferase 1 
       isoform X1 (BbDGAT1-X1) in pAT008, codon optimised for expression
       in yeast.

<400>  16
atgtgcccac aagaaggtaa aggttctcca ggtttgtctc catacaaaac tggtggtttg       60

gctcaatgtc caggttggcc accaggtggt agagttgttg gtggtagatg gggttgttgt      120

ttggaaccag cttctggtgc tggtccaggt ttatcttgtg ctccaccacc attgccacca      180

gctgaaggtt ggttgccatc tccaccagat ccagctttag gtgccggttt gtctgaagtt      240

ggttctgaag gtcctttgtt ggcttctgct gttgattcta gatgtcacag attgcaggat      300

tccttgttct cttctgattc tggtttctct aactacaggg gtattttgaa ctggtgcgtt      360

gttatgttga ttttgtctaa cgctaggctg ttcttggaga acttgattaa gtacggtatc      420

ttggttgatc caatccaggt tgtttctttg ttcttgaagg atccatattc ttggccagct      480

ttgtgtttgg ttatcgttgc taacattttt gctgttgctg ccttccaagt cgaaaaaaga      540

ttggctgttg gtgctttgac tgaacaagct ggtttgttgt tgcatggtgt taacttggct      600

accattttgt gttttccagc tgctgttgca tttctgttgg aatctattac tccagtcggt      660

tctgttttgg ctttgatggt ttacactatc ctgttcctaa agttgttctc ctacagagat      720

gttaatttgt ggtgcagaga aagacgtgct ggtgctaaag ctaaagctgc tttggctggt      780

aaaaaagcta acggtggtgc tgctcaaaga actgtttctt atccagataa cttgacctac      840

agggacttgt actacttttt gtttgctcca accttgtgct acgaattgaa ttttccaaga      900

tctcccagga tcagaaagag gtttttgttg agaagattgc tggaaatgct gtttttgacc      960

caattgcaag tcggtttgat tcaacaatgg atggttccag ctatccagaa ttctatgaag     1020

ccattcaagg atatggacta ctccagaatc gtcgaaaggt tgttgaaatt ggcagttcca     1080

aaccatttga tctggctgat ttttttctac tggttgttcc actcttgctt gaatgcagtt     1140

gctgaattga tgcaatttgg tgacagagaa ttttacagag actggtggaa ctctgaatcc     1200

attacttact tttggcagaa ctggaacatc ccagttcata agtggtgtat cagacatttc     1260

tacaagccaa tgcttagaag gggttcttct aaatgggctg ctagaacagc agtttttttg     1320

gcctctgctt ttttccacga atacttggtt tctatcccat tgagaatgtt cagattgtgg     1380

gcttttactg gtatgatggc tcaaattcca ttagcttgga tcgttggtag attcttcaga     1440

ggtaattatg gtaatgctgc cgtttggttg tccttgatta ttggtcaacc agttgctgtt     1500

ttgatgtacg ttcatgatta ctacgtcttg aacagagaag ctccagctgc aggtacttaa     1560


<210>  17
<211>  504
<212>  PRT
<213>  Bubalus bubalis

<400>  17

Met Cys Pro Gln Glu Gly Lys Gly Ser Pro Gly Leu Ser Pro Tyr Lys 
1               5                   10                  15      


Thr Gly Gly Leu Ala Gln Cys Pro Gly Trp Pro Pro Gly Gly Arg Val 
            20                  25                  30          


Val Gly Gly Arg Trp Gly Cys Cys Leu Glu Pro Ala Ser Gly Ala Gly 
        35                  40                  45              


Pro Gly Leu Ser Cys Ala Pro Pro Pro Leu Pro Pro Ala Glu Gly Trp 
    50                  55                  60                  


Leu Pro Ser Pro Pro Asp Pro Ala Leu Gly Ala Gly Leu Ser Glu Val 
65                  70                  75                  80  


Gly Ser Glu Gly Pro Leu Leu Ala Ser Ala Val Asp Ser Arg Cys His 
                85                  90                  95      


Arg Leu Gln Asp Ser Leu Phe Ser Ser Asp Ser Gly Phe Ser Asn Tyr 
            100                 105                 110         


Arg Gly Ile Leu Asn Trp Cys Val Val Met Leu Ile Leu Ser Asn Ala 
        115                 120                 125             


Arg Leu Phe Leu Glu Asn Leu Ile Lys Tyr Gly Ile Leu Val Asp Pro 
    130                 135                 140                 


Ile Gln Val Val Ser Leu Phe Leu Lys Asp Pro Tyr Ser Trp Pro Ala 
145                 150                 155                 160 


Leu Cys Leu Val Ile Val Ala Asn Ile Phe Ala Val Ala Ala Phe Gln 
                165                 170                 175     


Val Glu Lys Arg Leu Ala Val Gly Ala Leu Thr Glu Gln Ala Gly Leu 
            180                 185                 190         


Leu Leu His Gly Val Asn Leu Ala Thr Ile Leu Cys Phe Pro Ala Ala 
        195                 200                 205             


Val Ala Phe Leu Leu Glu Ser Ile Thr Pro Val Gly Ser Val Leu Ala 
    210                 215                 220                 


Leu Met Val Tyr Thr Ile Leu Phe Leu Lys Leu Phe Ser Tyr Arg Asp 
225                 230                 235                 240 


Val Asn Leu Trp Cys Arg Glu Arg Arg Ala Gly Ala Lys Ala Lys Ala 
                245                 250                 255     


Ala Leu Ala Gly Lys Lys Ala Asn Gly Gly Ala Ala Gln Arg Thr Val 
            260                 265                 270         


Ser Tyr Pro Asp Asn Leu Thr Tyr Arg Asp Leu Tyr Tyr Phe Leu Phe 
        275                 280                 285             


Ala Pro Thr Leu Cys Tyr Glu Leu Asn Phe Pro Arg Ser Pro Arg Ile 
    290                 295                 300                 


Arg Lys Arg Phe Leu Leu Arg Arg Leu Leu Glu Met Leu Phe Leu Thr 
305                 310                 315                 320 


Gln Leu Gln Val Gly Leu Ile Gln Gln Trp Met Val Pro Ala Ile Gln 
                325                 330                 335     


Asn Ser Met Lys Pro Phe Lys Val Pro Asn His Leu Ile Trp Leu Ile 
            340                 345                 350         


Phe Phe Tyr Trp Leu Phe His Ser Cys Leu Asn Ala Val Ala Glu Leu 
        355                 360                 365             


Met Gln Phe Gly Asp Arg Glu Phe Tyr Arg Asp Trp Trp Asn Ser Glu 
    370                 375                 380                 


Ser Ile Thr Tyr Phe Trp Gln Asn Trp Asn Ile Pro Val His Lys Trp 
385                 390                 395                 400 


Cys Ile Arg His Phe Tyr Lys Pro Met Leu Arg Arg Gly Ser Ser Lys 
                405                 410                 415     


Trp Ala Ala Arg Thr Ala Val Phe Leu Ala Ser Ala Phe Phe His Glu 
            420                 425                 430         


Tyr Leu Val Ser Ile Pro Leu Arg Met Phe Arg Leu Trp Ala Phe Thr 
        435                 440                 445             


Gly Met Met Ala Gln Ile Pro Leu Ala Trp Ile Val Gly Arg Phe Phe 
    450                 455                 460                 


Arg Gly Asn Tyr Gly Asn Ala Ala Val Trp Leu Ser Leu Ile Ile Gly 
465                 470                 475                 480 


Gln Pro Val Ala Val Leu Met Tyr Val His Asp Tyr Tyr Val Leu Asn 
                485                 490                 495     


Arg Glu Ala Pro Ala Ala Gly Thr 
            500                 


<210>  18
<211>  1515
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of the protein coding region of Bubulus 
       bubulis (water buffalo) diacylglycerol O-acyltransferase 1 
       isoform X2 (BbDGAT1-X2) in pAT009, codon optimised for expression
       in yeast.

<400>  18
atgtgcccac aagaaggtaa aggttctcca ggtttgtctc catacaaaac tggtggtttg       60

gctcaatgtc caggttggcc accaggtggt agagttgttg gtggtagatg gggttgttgt      120

ttggaaccag cttctggtgc tggtccaggt ttatcttgtg ctccaccacc attgccacca      180

gctgaaggtt ggttgccatc tccaccagat ccagctttag gtgccggttt gtctgaagtt      240

ggttctgaag gtcctttgtt ggcttctgct gttgattcta gatgtcacag attgcaggat      300

tccttgttct cttctgattc tggtttctct aactacaggg gtattttgaa ctggtgcgtt      360

gttatgttga ttttgtctaa cgctaggctg ttcttggaga acttgattaa gtacggtatc      420

ttggttgatc caatccaggt tgtttctttg ttcttgaagg atccatattc ttggccagct      480

ttgtgtttgg ttatcgttgc taacattttt gctgttgctg ccttccaagt cgaaaaaaga      540

ttggctgttg gtgctttgac tgaacaagct ggtttgttgt tgcatggtgt taacttggct      600

accattttgt gttttccagc tgctgttgca tttctgttgg aatctattac tccagtcggt      660

tctgttttgg ctttgatggt ttacactatc ctgttcctaa agttgttctc ctacagagat      720

gttaatttgt ggtgcagaga aagacgtgct ggtgctaaag ctaaagctgc tttggctggt      780

aaaaaagcta acggtggtgc tgctcaaaga actgtttctt atccagataa cttgacctac      840

agggacttgt actacttttt gtttgctcca accttgtgct acgaattgaa ttttccaaga      900

tctcccagga tcagaaagag gtttttgttg agaagattgc tggaaatgct gtttttgacc      960

caattgcaag tcggtttgat tcaacaatgg atggttccag ctatccagaa ttctatgaag     1020

ccattcaagg ttcctaacca cttgatctgg ttgattttct tctactggtt gttccactct     1080

tgcttgaatg cagttgctga attgatgcaa tttggtgaca gagaatttta cagagactgg     1140

tggaactctg aatccattac ttacttttgg cagaactgga acatcccagt tcataagtgg     1200

tgtatcagac atttctacaa gccaatgctt agaaggggtt cttctaaatg ggctgctaga     1260

acagcagttt ttttggcctc tgcttttttc cacgaatact tggtttctat cccattgaga     1320

atgttcagat tgtgggcttt tactggtatg atggctcaaa ttccattagc ttggatcgtt     1380

ggtagattct tcagaggtaa ttatggtaat gctgccgttt ggttgtcctt gattattggt     1440

caaccagttg ctgttttgat gtacgttcat gattactacg tcttgaacag agaagctcca     1500

gctgcaggta cttaa                                                      1515


<210>  19
<211>  499
<212>  PRT
<213>  Bubalus bubalis

<400>  19

Met Arg Trp Leu Trp Asp Gly Ser Ser Asp Ser Glu Trp Ala Gly Tyr 
1               5                   10                  15      


Gly Gly Phe Trp Ala Gly Pro Ser Ser Gly Tyr Pro His Arg Leu Ser 
            20                  25                  30          


Ser Trp Asp Ser Trp Ala Trp Glu Pro Cys Ser Pro Cys Gln Lys Ala 
        35                  40                  45              


Gly Ala Glu Ser Arg Arg Arg Gly Pro Gly Gly Ser Glu Pro His Cys 
    50                  55                  60                  


Val Cys Ser Pro Gly Trp Thr Trp Arg Arg Cys His Arg Leu Gln Asp 
65                  70                  75                  80  


Ser Leu Phe Ser Ser Asp Ser Gly Phe Ser Asn Tyr Arg Gly Ile Leu 
                85                  90                  95      


Asn Trp Cys Val Val Met Leu Ile Leu Ser Asn Ala Arg Leu Phe Leu 
            100                 105                 110         


Glu Asn Leu Ile Lys Tyr Gly Ile Leu Val Asp Pro Ile Gln Val Val 
        115                 120                 125             


Ser Leu Phe Leu Lys Asp Pro Tyr Ser Trp Pro Ala Leu Cys Leu Val 
    130                 135                 140                 


Ile Val Ala Asn Ile Phe Ala Val Ala Ala Phe Gln Val Glu Lys Arg 
145                 150                 155                 160 


Leu Ala Val Gly Ala Leu Thr Glu Gln Ala Gly Leu Leu Leu His Gly 
                165                 170                 175     


Val Asn Leu Ala Thr Ile Leu Cys Phe Pro Ala Ala Val Ala Phe Leu 
            180                 185                 190         


Leu Glu Ser Ile Thr Pro Val Gly Ser Val Leu Ala Leu Met Val Tyr 
        195                 200                 205             


Thr Ile Leu Phe Leu Lys Leu Phe Ser Tyr Arg Asp Val Asn Leu Trp 
    210                 215                 220                 


Cys Arg Glu Arg Arg Ala Gly Ala Lys Ala Lys Ala Ala Leu Ala Gly 
225                 230                 235                 240 


Lys Lys Ala Asn Gly Gly Ala Ala Gln Arg Thr Val Ser Tyr Pro Asp 
                245                 250                 255     


Asn Leu Thr Tyr Arg Asp Leu Tyr Tyr Phe Leu Phe Ala Pro Thr Leu 
            260                 265                 270         


Cys Tyr Glu Leu Asn Phe Pro Arg Ser Pro Arg Ile Arg Lys Arg Phe 
        275                 280                 285             


Leu Leu Arg Arg Leu Leu Glu Met Leu Phe Leu Thr Gln Leu Gln Val 
    290                 295                 300                 


Gly Leu Ile Gln Gln Trp Met Val Pro Ala Ile Gln Asn Ser Met Lys 
305                 310                 315                 320 


Pro Phe Lys Asp Met Asp Tyr Ser Arg Ile Val Glu Arg Leu Leu Lys 
                325                 330                 335     


Leu Ala Val Pro Asn His Leu Ile Trp Leu Ile Phe Phe Tyr Trp Leu 
            340                 345                 350         


Phe His Ser Cys Leu Asn Ala Val Ala Glu Leu Met Gln Phe Gly Asp 
        355                 360                 365             


Arg Glu Phe Tyr Arg Asp Trp Trp Asn Ser Glu Ser Ile Thr Tyr Phe 
    370                 375                 380                 


Trp Gln Asn Trp Asn Ile Pro Val His Lys Trp Cys Ile Arg His Phe 
385                 390                 395                 400 


Tyr Lys Pro Met Leu Arg Arg Gly Ser Ser Lys Trp Ala Ala Arg Thr 
                405                 410                 415     


Ala Val Phe Leu Ala Ser Ala Phe Phe His Glu Tyr Leu Val Ser Ile 
            420                 425                 430         


Pro Leu Arg Met Phe Arg Leu Trp Ala Phe Thr Gly Met Met Ala Gln 
        435                 440                 445             


Ile Pro Leu Ala Trp Ile Val Gly Arg Phe Phe Arg Gly Asn Tyr Gly 
    450                 455                 460                 


Asn Ala Ala Val Trp Leu Ser Leu Ile Ile Gly Gln Pro Val Ala Val 
465                 470                 475                 480 


Leu Met Tyr Val His Asp Tyr Tyr Val Leu Asn Arg Glu Ala Pro Ala 
                485                 490                 495     


Ala Gly Thr 
            


<210>  20
<211>  1500
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of the protein coding region of Bubulus 
       bubulis (water buffalo) diacylglycerol O-acyltransferase 1 
       isoform X3 (BbDGAT1-X3) in pAT010, codon optimised for expression
       in yeast.

<400>  20
atgaggtggt tgtgggatgg ttcctctgat tctgaatggg ctggttatgg tggtttttgg       60

gctggtccat cttctggtta tccacataga ttgtcatctt gggattcttg ggcttgggaa      120

ccatgttctc catgtcaaaa agctggtgct gaatctagaa gaagaggtcc aggcggttct      180

gaaccacatt gtgtttgttc tccaggttgg acttggagaa gatgtcatag attgcaagac      240

agcttgttct cttccgattc tggtttttct aactacaggg gtattttgaa ctggtgcgtt      300

gttatgttga ttttgtctaa cgctaggctg ttcttggaga acttgattaa gtacggtatc      360

ttggttgatc caatccaggt tgtttctttg ttcttgaagg atccatattc ttggccagct      420

ttgtgtttgg ttatcgttgc taacattttt gctgttgctg ccttccaagt cgaaaaaaga      480

ttggctgttg gtgctttgac tgaacaagca ggtttgttgt tgcatggtgt taacttggct      540

accattttgt gttttccagc tgctgttgca ttcttgttgg aatctattac tccagttggt      600

tccgttttgg ctttgatggt ttacactatc ctgttcctaa agctgttctc ctacagagat      660

gttaatttgt ggtgtagaga aagacgtgct ggtgctaaag ctaaagctgc tttggctggt      720

aaaaaagcta atggtggtgc tgctcaaaga actgtttctt acccagataa cttgacttac      780

agggacttgt actacttttt gttcgctcca actttgtgct acgaattgaa ttttccaaga      840

tctcccagga tcagaaagag gtttttgttg agaagattgc tggaaatgct gtttttgacc      900

caattgcaag tcggtttgat tcaacaatgg atggtcccag ctattcagaa ttctatgaag      960

ccattcaagg acatggacta ctctagaatc gtcgaaaggt tgttgaaatt ggccgttcca     1020

aaccatttga tctggctgat ttttttctac tggttgttcc actcttgctt gaatgcagtt     1080

gctgaattga tgcaatttgg tgacagagaa ttttacagag actggtggaa ctctgaatcc     1140

attacttact tttggcagaa ctggaacatc ccagttcata agtggtgtat cagacatttc     1200

tacaagccaa tgcttagaag gggttcttca aaatgggctg ctagaacagc agtttttttg     1260

gcttctgctt tcttccacga atacttggtt tctatcccat tgagaatgtt cagattgtgg     1320

gcttttactg gtatgatggc tcaaattcca ttggcatgga tagttggtag attcttcaga     1380

ggtaattacg gtaatgctgc tgtttggttg tccttgatta ttggtcaacc agttgctgtt     1440

ttgatgtacg ttcatgatta ctacgtcttg aacagagaag ctccagctgc aggtacttaa     1500


<210>  21
<211>  497
<212>  PRT
<213>  Bubalus bubalis

<400>  21

Met Cys Pro Gln Glu Gly Lys Gly Ser Pro Gly Leu Ser Pro Tyr Lys 
1               5                   10                  15      


Thr Gly Gly Leu Ala Gln Cys Pro Gly Trp Pro Pro Gly Gly Arg Val 
            20                  25                  30          


Val Gly Gly Arg Trp Gly Cys Cys Leu Glu Pro Ala Ser Gly Ala Gly 
        35                  40                  45              


Pro Gly Leu Ser Cys Ala Pro Pro Pro Leu Pro Pro Ala Glu Gly Trp 
    50                  55                  60                  


Leu Pro Ser Pro Pro Asp Pro Ala Leu Gly Ala Gly Leu Ser Glu Val 
65                  70                  75                  80  


Gly Ser Glu Gly Pro Leu Leu Ala Ser Ala Val Asp Ser Arg Cys His 
                85                  90                  95      


Arg Leu Gln Asp Ser Leu Phe Ser Ser Asp Ser Gly Phe Ser Asn Tyr 
            100                 105                 110         


Arg Gly Ile Leu Asn Trp Cys Val Val Met Leu Ile Leu Ser Asn Ala 
        115                 120                 125             


Arg Leu Phe Leu Glu Asn Leu Ile Lys Tyr Gly Ile Leu Val Asp Pro 
    130                 135                 140                 


Ile Gln Val Val Ser Leu Phe Leu Lys Asp Pro Tyr Ser Trp Pro Ala 
145                 150                 155                 160 


Leu Cys Leu Val Ile Val Ala Asn Ile Phe Ala Val Ala Ala Phe Gln 
                165                 170                 175     


Val Glu Lys Arg Leu Ala Val Gly Ala Leu Thr Glu Gln Ala Gly Leu 
            180                 185                 190         


Leu Leu His Gly Val Asn Leu Ala Thr Ile Leu Cys Phe Pro Ala Ala 
        195                 200                 205             


Val Ala Phe Leu Leu Glu Ser Ile Thr Pro Val Gly Ser Val Leu Ala 
    210                 215                 220                 


Leu Met Val Tyr Thr Ile Leu Phe Leu Lys Leu Phe Ser Tyr Arg Asp 
225                 230                 235                 240 


Val Asn Leu Trp Cys Arg Glu Arg Arg Ala Gly Ala Lys Ala Lys Ala 
                245                 250                 255     


Ala Leu Ala Asp Leu Tyr Tyr Phe Leu Phe Ala Pro Thr Leu Cys Tyr 
            260                 265                 270         


Glu Leu Asn Phe Pro Arg Ser Pro Arg Ile Arg Lys Arg Phe Leu Leu 
        275                 280                 285             


Arg Arg Leu Leu Glu Met Leu Phe Leu Thr Gln Leu Gln Val Gly Leu 
    290                 295                 300                 


Ile Gln Gln Trp Met Val Pro Ala Ile Gln Asn Ser Met Lys Pro Phe 
305                 310                 315                 320 


Lys Asp Met Asp Tyr Ser Arg Ile Val Glu Arg Leu Leu Lys Leu Ala 
                325                 330                 335     


Val Pro Asn His Leu Ile Trp Leu Ile Phe Phe Tyr Trp Leu Phe His 
            340                 345                 350         


Ser Cys Leu Asn Ala Val Ala Glu Leu Met Gln Phe Gly Asp Arg Glu 
        355                 360                 365             


Phe Tyr Arg Asp Trp Trp Asn Ser Glu Ser Ile Thr Tyr Phe Trp Gln 
    370                 375                 380                 


Asn Trp Asn Ile Pro Val His Lys Trp Cys Ile Arg His Phe Tyr Lys 
385                 390                 395                 400 


Pro Met Leu Arg Arg Gly Ser Ser Lys Trp Ala Ala Arg Thr Ala Val 
                405                 410                 415     


Phe Leu Ala Ser Ala Phe Phe His Glu Tyr Leu Val Ser Ile Pro Leu 
            420                 425                 430         


Arg Met Phe Arg Leu Trp Ala Phe Thr Gly Met Met Ala Gln Ile Pro 
        435                 440                 445             


Leu Ala Trp Ile Val Gly Arg Phe Phe Arg Gly Asn Tyr Gly Asn Ala 
    450                 455                 460                 


Ala Val Trp Leu Ser Leu Ile Ile Gly Gln Pro Val Ala Val Leu Met 
465                 470                 475                 480 


Tyr Val His Asp Tyr Tyr Val Leu Asn Arg Glu Ala Pro Ala Ala Gly 
                485                 490                 495     


Thr 
    


<210>  22
<211>  1494
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of the protein coding region of Bubulus 
       bubulis (water buffalo) diacylglycerol O-acyltransferase 1 
       isoform X4 (BbDGAT1-X4) in pAT011, codon optimised for expression
       in yeast.

<400>  22
atgtgcccac aagaaggtaa aggttctcca ggtttgtctc catacaaaac tggtggtttg       60

gctcaatgtc caggttggcc accaggtggt agagttgttg gtggtagatg gggttgttgt      120

ttggaaccag cttctggtgc tggtccaggt ttatcttgtg ctccaccacc attgccacca      180

gctgaaggtt ggttgccatc tccaccagat ccagctttag gtgccggttt gtctgaagtt      240

ggttctgaag gtcctttgtt ggcttctgct gttgattcta gatgtcacag attgcaggat      300

tccttgttct cttctgattc tggtttctct aactacaggg gtattttgaa ctggtgcgtt      360

gttatgttga ttttgtctaa cgctaggctg ttcttggaga acttgattaa gtacggtatc      420

ttggttgatc caatccaggt tgtttctttg ttcttgaagg atccatattc ttggccagct      480

ttgtgtttgg ttatcgttgc taacattttt gctgttgctg ccttccaagt cgaaaaaaga      540

ttggctgttg gtgctttgac tgaacaagct ggtttgttgt tgcatggtgt taacttggct      600

accattttgt gttttccagc tgctgttgca tttctgttgg aatctattac tccagtcggt      660

tctgttttgg ctttgatggt ttacactatc ctgttcctaa agttgttctc ctacagagat      720

gttaatttgt ggtgcagaga aagacgtgct ggtgctaaag ctaaagctgc tttggctgac      780

ttgtactact ttttgtttgc tccaaccttg tgctacgaat tgaattttcc aagatctccc      840

aggatcagaa agaggttttt gttgagaaga ttgctggaaa tgctgttttt gacccaattg      900

caagtcggtt tgattcaaca atggatggtt ccagctatcc agaattctat gaagccattc      960

aaggatatgg actactccag aatcgtcgaa aggttgttga aattggcagt tccaaaccat     1020

ttgatctggc tgattttttt ctactggttg ttccactctt gcttgaatgc agttgctgaa     1080

ttgatgcaat ttggtgacag agaattttac agagactggt ggaactctga atccattact     1140

tacttttggc agaactggaa catcccagtt cataagtggt gtatcagaca tttctacaag     1200

ccaatgctta gaaggggttc ttctaaatgg gctgctagaa cagcagtttt tttggcctct     1260

gcttttttcc acgaatactt ggtttctatc ccattgagaa tgttcagatt gtgggctttt     1320

actggtatga tggctcaaat tccattagct tggatcgttg gtagattctt cagaggtaat     1380

tatggtaatg ctgccgtttg gttgtccttg attattggtc aaccagttgc tgttttgatg     1440

tacgttcatg attactacgt cttgaacaga gaagctccag ctgcaggtac ttaa           1494


<210>  23
<211>  474
<212>  PRT
<213>  Bubalus bubalis

<400>  23

Met Cys Pro Gln Glu Gly Lys Gly Ser Pro Gly Leu Ser Pro Tyr Lys 
1               5                   10                  15      


Thr Gly Gly Leu Ala Gln Cys Pro Gly Trp Pro Pro Gly Gly Arg Val 
            20                  25                  30          


Val Gly Gly Arg Trp Gly Cys Cys Leu Glu Pro Ala Ser Gly Ala Gly 
        35                  40                  45              


Pro Gly Leu Ser Cys Ala Pro Pro Pro Leu Pro Pro Ala Glu Gly Trp 
    50                  55                  60                  


Leu Pro Ser Pro Pro Asp Pro Ala Leu Gly Ala Gly Leu Ser Glu Val 
65                  70                  75                  80  


Gly Ser Glu Gly Pro Leu Leu Ala Ser Ala Val Asp Ser Arg Cys His 
                85                  90                  95      


Arg Leu Gln Asp Ser Leu Phe Ser Ser Asp Ser Gly Phe Ser Asn Tyr 
            100                 105                 110         


Arg Gly Ile Leu Asn Trp Cys Val Val Met Leu Ile Leu Ser Asn Ala 
        115                 120                 125             


Arg Leu Phe Leu Glu Asn Leu Ile Lys Tyr Gly Ile Leu Val Asp Pro 
    130                 135                 140                 


Ile Gln Val Val Ser Leu Phe Leu Lys Asp Pro Tyr Ser Trp Pro Ala 
145                 150                 155                 160 


Leu Cys Leu Val Ile Val Ala Asn Ile Phe Ala Val Ala Ala Phe Gln 
                165                 170                 175     


Val Glu Lys Arg Leu Ala Val Gly Ala Leu Thr Glu Gln Ala Gly Leu 
            180                 185                 190         


Leu Leu His Gly Val Asn Leu Ala Thr Ile Leu Cys Phe Pro Ala Ala 
        195                 200                 205             


Val Ala Phe Leu Leu Glu Ser Ile Thr Pro Ala Val Leu Leu Pro Gly 
    210                 215                 220                 


Arg Gln Pro Leu Val Pro Arg Ala Gln Gly Trp Gly Gln Gly Gln Gly 
225                 230                 235                 240 


Cys Phe Gly Arg Ser Leu Leu Leu Pro Leu Arg Pro Asp Pro Val Leu 
                245                 250                 255     


Arg Ala Gln Leu Pro Pro Leu Pro Pro His Pro Lys Ala Leu Pro Ala 
            260                 265                 270         


Ala Ala Thr Pro Gly Asp Ala Val Pro His Pro Ala Pro Gly Gly Ala 
        275                 280                 285             


Asp Pro Ala Val Asp Gly Pro Gly His Pro Glu Leu His Glu Ala Leu 
    290                 295                 300                 


Gln Gly His Gly Leu Leu Pro His Arg Gly Ala Pro Pro Glu Ala Gly 
305                 310                 315                 320 


Gly Pro Gln Pro Pro His Leu Ala His Leu Leu Leu Leu Ala Leu Pro 
                325                 330                 335     


Leu Leu Pro Glu Arg Arg Gly Arg Ala His Ala Val Trp Arg Pro Arg 
            340                 345                 350         


Val Leu Pro Gly Leu Val Glu Leu Arg Val Tyr His Leu Leu Leu Ala 
        355                 360                 365             


Glu Leu Glu His Pro Cys Ser Gln Val Val His Gln Thr Leu Leu Gln 
    370                 375                 380                 


Ala His Ala Pro Ala Gly Gln Gln Gln Val Gly Ser Gln Asp Gly Ser 
385                 390                 395                 400 


Ile Ser Gly Leu Arg Leu Leu Pro Arg Val Pro Gly Glu His Pro Pro 
                405                 410                 415     


Ala His Val Pro Pro Leu Gly Leu His Gly His Asp Gly Ala Asp Pro 
            420                 425                 430         


Ala Gly Leu Asp Ser Gly Pro Leu Leu Pro Arg Gln Leu Arg Gln Arg 
        435                 440                 445             


Ser Arg Val Ala Val Thr His His Trp Ala Ala Gly Gly Arg Pro Asp 
    450                 455                 460                 


Val Arg Pro Arg Leu Leu Arg Ala Gln Pro 
465                 470                 


<210>  24
<211>  1425
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of the protein coding region of Bubulus 
       bubulis (water buffalo) diacylglycerol O-acyltransferase 1 
       isoform X5 (BbDGAT1-X5) in pAT012, codon optimised for expression
       in yeast.

<400>  24
atgtgcccac aagaaggtaa aggttctcca ggtttgtctc catacaaaac tggtggtttg       60

gctcaatgtc caggttggcc accaggtggt agagttgttg gtggtagatg gggttgttgt      120

ttggaaccag cttctggtgc tggtccaggt ttatcttgtg ctccaccacc attgccacca      180

gctgaaggtt ggttgccatc tccaccagat ccagctttag gtgccggttt gtctgaagtt      240

ggttctgaag gtcctttgtt ggcttctgct gttgattcta gatgtcacag attgcaggat      300

tccttgttct cttctgattc tggtttctct aactacaggg gtattttgaa ctggtgcgtt      360

gttatgttga ttttgtctaa cgctaggctg ttcttggaga acttgattaa gtacggtatc      420

ttggttgatc caatccaggt tgtttctttg ttcttgaagg atccatattc ttggccagct      480

ttgtgtttgg ttatcgttgc taacattttt gctgttgctg ccttccaagt cgaaaaaaga      540

ttggctgttg gtgctttgac tgaacaagct ggtttgttgt tgcatggtgt taacttggct      600

accattttgt gttttccagc tgctgttgca tttctgttgg aatctattac tccagctgtt      660

ttgttgccag gtagacaacc attggttcca agagcacaag gttggggtca aggtcaaggt      720

tgttttggta gatctttgtt gttaccattg aggccagatc ctgttttgag agcacaattg      780

ccacctttac caccacatcc aaaggctttg cctgctgctg ctactccagg tgatgctgtt      840

ccacatccag ctccaggtgg tgcagatcct gcagttgatg gtcctggtca tccagaattg      900

catgaagcct tacaaggtca tggtttgtta ccacatagag gtgccccacc tgaagccggt      960

ggtccacaac cacctcattt ggctcatttg ttattattgg cattgcctct gttgccagag     1020

agaagaggta gagcacatgc tgtttggagg ccaagagttt tgccaggttt ggttgaattg     1080

agagtttacc acttgttgtt ggccgaattg gaacatccat gttctcaagt tgttcaccaa     1140

actttgttgc aagctcatgc tccagctggt caacaacagg ttggttctca agatggttct     1200

atttccggtt tgagattatt gcctagagtt ccaggtgaac atccaccagc tcatgttcca     1260

ccattgggtt tacatggtca tgatggtgct gacccagctg gtttagattc tggtccatta     1320

ttgccaagac aattgagaca gagatctaga gttgctgtta ctcatcattg ggctgccggt     1380

ggtagaccag atgttagacc aagattattg agagcccaac cataa                     1425


<210>  25
<211>  467
<212>  PRT
<213>  Bubalus bubalis

<400>  25

Met Gly Asp Arg Gly Gly Ala Gly Gly Ser Arg Arg Arg Arg Thr Gly 
1               5                   10                  15      


Ser Arg Pro Ser Ile Gln Gly Gly Ser Gly Pro Ala Ala Val Glu Glu 
            20                  25                  30          


Glu Val Arg Asp Ala Gly Ala Gly Gly Asp Ala Pro Val Arg Asp Thr 
        35                  40                  45              


Asp Lys Asp Gly Asp Val Asp Val Gly Ser Gly His Trp Asp Leu Arg 
    50                  55                  60                  


Cys His Arg Leu Gln Asp Ser Leu Phe Ser Ser Asp Ser Gly Phe Ser 
65                  70                  75                  80  


Asn Tyr Arg Gly Ile Leu Asn Trp Cys Val Val Met Leu Ile Leu Ser 
                85                  90                  95      


Asn Ala Arg Leu Phe Leu Glu Asn Leu Ile Lys Tyr Gly Ile Leu Val 
            100                 105                 110         


Asp Pro Ile Gln Val Val Ser Leu Phe Leu Lys Asp Pro Tyr Ser Trp 
        115                 120                 125             


Pro Ala Leu Cys Leu Val Ile Val Ala Asn Ile Phe Ala Val Ala Ala 
    130                 135                 140                 


Phe Gln Val Glu Lys Arg Leu Ala Val Gly Ala Leu Thr Glu Gln Ala 
145                 150                 155                 160 


Gly Leu Leu Leu His Gly Val Asn Leu Ala Thr Ile Leu Cys Phe Pro 
                165                 170                 175     


Ala Ala Val Ala Phe Leu Leu Glu Ser Ile Thr Pro Val Gly Ser Val 
            180                 185                 190         


Leu Ala Leu Met Val Tyr Thr Ile Leu Phe Leu Lys Leu Phe Ser Tyr 
        195                 200                 205             


Arg Asp Val Asn Leu Trp Cys Arg Glu Arg Arg Ala Gly Ala Lys Ala 
    210                 215                 220                 


Lys Ala Ala Leu Ala Asp Leu Tyr Tyr Phe Leu Phe Ala Pro Thr Leu 
225                 230                 235                 240 


Cys Tyr Glu Leu Asn Phe Pro Arg Ser Pro Arg Ile Arg Lys Arg Phe 
                245                 250                 255     


Leu Leu Arg Arg Leu Leu Glu Met Leu Phe Leu Thr Gln Leu Gln Val 
            260                 265                 270         


Gly Leu Ile Gln Gln Trp Met Val Pro Ala Ile Gln Asn Ser Met Lys 
        275                 280                 285             


Pro Phe Lys Asp Met Asp Tyr Ser Arg Ile Val Glu Arg Leu Leu Lys 
    290                 295                 300                 


Leu Ala Val Pro Asn His Leu Ile Trp Leu Ile Phe Phe Tyr Trp Leu 
305                 310                 315                 320 


Phe His Ser Cys Leu Asn Ala Val Ala Glu Leu Met Gln Phe Gly Asp 
                325                 330                 335     


Arg Glu Phe Tyr Arg Asp Trp Trp Asn Ser Glu Ser Ile Thr Tyr Phe 
            340                 345                 350         


Trp Gln Asn Trp Asn Ile Pro Val His Lys Trp Cys Ile Arg His Phe 
        355                 360                 365             


Tyr Lys Pro Met Leu Arg Arg Gly Ser Ser Lys Trp Ala Ala Arg Thr 
    370                 375                 380                 


Ala Val Phe Leu Ala Ser Ala Phe Phe His Glu Tyr Leu Val Ser Ile 
385                 390                 395                 400 


Pro Leu Arg Met Phe Arg Leu Trp Ala Phe Thr Gly Met Met Ala Gln 
                405                 410                 415     


Ile Pro Leu Ala Trp Ile Val Gly Arg Phe Phe Arg Gly Asn Tyr Gly 
            420                 425                 430         


Asn Ala Ala Val Trp Leu Ser Leu Ile Ile Gly Gln Pro Val Ala Val 
        435                 440                 445             


Leu Met Tyr Val His Asp Tyr Tyr Val Leu Asn Arg Glu Ala Pro Ala 
    450                 455                 460                 


Ala Gly Thr 
465         


<210>  26
<211>  1404
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of the protein coding region of Bubulus 
       bubulis (water buffalo) diacylglycerol O-acyltransferase 1 
       isoform X6 (BbDGAT1-X6) in pAT013, codon optimised for expression
       in yeast.

<400>  26
atgggtgata gaggtggtgc tggtggttct agaagaagaa ggacaggttc tagaccatct       60

attcaaggtg gttctggtcc agctgctgtt gaagaagagg ttagagatgc tggtgccggt      120

ggtgatgctc cagttagaga tactgataag gatggtgatg ttgatgttgg ttcaggtcat      180

tgggatttga gatgtcatag attgcaggac agcttgttct cttctgattc tggtttttct      240

aactacaggg gtattttgaa ctggtgcgtt gttatgttga ttttgtctaa cgctaggctg      300

ttcttggaga acttgattaa gtacggtatc ttggttgatc caatccaggt tgtttctttg      360

ttcttgaagg atccatattc ttggccagct ttgtgtttgg ttatcgttgc taacattttt      420

gctgttgctg ccttccaagt cgaaaaaaga ttggctgttg gtgctttgac tgaacaagca      480

ggtttgttgt tgcatggtgt taacttggct accattttgt gttttccagc agcagttgct      540

ttcttgttgg aatctattac tccagttggt tccgttttgg ctttgatggt ttacactatc      600

ctgttcctaa agctgttctc ctacagagat gttaatttgt ggtgtagaga aagacgtgct      660

ggtgctaaag ctaaagctgc tttggctgac ttgtactact ttttgtttgc tccaaccttg      720

tgctacgaat tgaattttcc aagatctccc aggatcagaa agaggttttt gttgagaaga      780

ttgctggaaa tgctgttttt gacccaattg caagtcggtt tgattcaaca atggatggtt      840

ccagctatcc agaattctat gaagccattc aaggatatgg actactccag aatcgtcgaa      900

aggttgttga aattggcagt tccaaaccat ttgatctggc tgattttttt ctactggttg      960

ttccactctt gcttgaatgc tgttgcagaa ttgatgcaat ttggtgacag agaattttac     1020

agagactggt ggaactctga atccattact tacttttggc agaactggaa catcccagtt     1080

cataagtggt gtatcagaca tttctacaag ccaatgttgc gtagaggttc ttctaaatgg     1140

gctgctagaa cagcagtttt tttggcttct gctttcttcc acgaatactt ggtttctatc     1200

ccattgagaa tgttcagatt gtgggctttt actggtatga tggctcaaat tccattagct     1260

tggatcgttg gtagattctt cagaggtaat tatggtaatg ctgccgtttg gttgtccttg     1320

attattggtc aaccagttgc tgttttgatg tacgttcatg attactacgt cttgaacaga     1380

gaagctccag ctgcaggtac ttaa                                            1404


<210>  27
<211>  459
<212>  PRT
<213>  Bubalus bubalis

<400>  27

Met Cys Pro Gln Glu Gly Lys Gly Ser Pro Gly Leu Ser Pro Tyr Lys 
1               5                   10                  15      


Thr Gly Gly Leu Ala Gln Cys Pro Gly Trp Pro Pro Gly Gly Arg Val 
            20                  25                  30          


Val Gly Gly Arg Trp Gly Cys Cys Leu Glu Pro Ala Ser Gly Ala Gly 
        35                  40                  45              


Pro Gly Leu Ser Cys Ala Pro Pro Pro Leu Pro Pro Ala Glu Gly Trp 
    50                  55                  60                  


Leu Pro Ser Pro Pro Asp Pro Ala Leu Gly Ala Gly Leu Ser Glu Val 
65                  70                  75                  80  


Gly Ser Glu Gly Pro Leu Leu Ala Ser Ala Val Asp Ser Arg Cys His 
                85                  90                  95      


Arg Leu Gln Asp Ser Leu Phe Ser Ser Asp Ser Gly Phe Ser Asn Tyr 
            100                 105                 110         


Arg Gly Ile Leu Asn Trp Cys Val Val Met Leu Ile Leu Ser Asn Ala 
        115                 120                 125             


Arg Leu Phe Leu Glu Asn Leu Ile Lys Tyr Gly Ile Leu Val Asp Pro 
    130                 135                 140                 


Ile Gln Val Val Ser Leu Phe Leu Lys Asp Pro Tyr Ser Trp Pro Ala 
145                 150                 155                 160 


Leu Cys Leu Val Ile Val Ala Asn Ile Phe Ala Val Ala Ala Phe Gln 
                165                 170                 175     


Val Glu Lys Arg Leu Ala Val Gly Ala Leu Thr Glu Gln Ala Gly Leu 
            180                 185                 190         


Leu Leu His Gly Val Asn Leu Ala Thr Ile Leu Cys Phe Pro Ala Ala 
        195                 200                 205             


Val Ala Phe Leu Leu Glu Ser Ile Thr Pro Ala Val Leu Leu Pro Gly 
    210                 215                 220                 


Arg Gln Pro Leu Val Pro Arg Ala Gln Gly Trp Gly Gln Gly Gln Gly 
225                 230                 235                 240 


Cys Phe Gly Arg Ser Leu Leu Leu Pro Leu Arg Pro Asp Pro Val Leu 
                245                 250                 255     


Arg Ala Gln Leu Pro Pro Leu Pro Pro His Pro Lys Ala Leu Pro Ala 
            260                 265                 270         


Ala Ala Thr Pro Gly Asp Ala Val Pro His Pro Ala Pro Gly Gly Ala 
        275                 280                 285             


Asp Pro Ala Val Asp Gly Pro Gly His Pro Glu Leu His Glu Ala Leu 
    290                 295                 300                 


Gln Gly Pro Gln Pro Pro His Leu Ala His Leu Leu Leu Leu Ala Leu 
305                 310                 315                 320 


Pro Leu Leu Pro Glu Arg Arg Gly Arg Ala His Ala Val Trp Arg Pro 
                325                 330                 335     


Arg Val Leu Pro Gly Leu Val Glu Leu Arg Val Tyr His Leu Leu Leu 
            340                 345                 350         


Ala Glu Leu Glu His Pro Cys Ser Gln Val Val His Gln Thr Leu Leu 
        355                 360                 365             


Gln Ala His Ala Pro Ala Gly Gln Gln Gln Val Gly Ser Gln Asp Gly 
    370                 375                 380                 


Ser Ile Ser Gly Leu Arg Leu Leu Pro Arg Val Pro Gly Glu His Pro 
385                 390                 395                 400 


Pro Ala His Val Pro Pro Leu Gly Leu His Gly His Asp Gly Ala Asp 
                405                 410                 415     


Pro Ala Gly Leu Asp Ser Gly Pro Leu Leu Pro Arg Gln Leu Arg Gln 
            420                 425                 430         


Arg Ser Arg Val Ala Val Thr His His Trp Ala Ala Gly Gly Arg Pro 
        435                 440                 445             


Asp Val Arg Pro Arg Leu Leu Arg Ala Gln Pro 
    450                 455                 


<210>  28
<211>  1380
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of the protein coding region of Bubulus 
       bubulis (water buffalo) diacylglycerol O-acyltransferase 1 
       isoform X7 (BbDGAT1-X7) in pAT014, codon optimised for expression
       in yeast.

<400>  28
atgtgcccac aagaaggtaa aggttctcca ggtttgtctc catacaaaac tggtggtttg       60

gctcaatgtc caggttggcc accaggtggt agagttgttg gtggtagatg gggttgttgt      120

ttggaaccag cttctggtgc tggtccaggt ttatcttgtg ctccaccacc attgccacca      180

gctgaaggtt ggttgccatc tccaccagat ccagctttag gtgccggttt gtctgaagtt      240

ggttctgaag gtcctttgtt ggcttctgct gttgattcta gatgtcacag attgcaggat      300

tccttgttct cttctgattc tggtttctct aactacaggg gtattttgaa ctggtgcgtt      360

gttatgttga ttttgtctaa cgctaggctg ttcttggaga acttgattaa gtacggtatc      420

ttggttgatc caatccaggt tgtttctttg ttcttgaagg atccatattc ttggccagct      480

ttgtgtttgg ttatcgttgc taacattttt gctgttgctg ccttccaagt cgaaaaaaga      540

ttggctgttg gtgctttgac tgaacaagct ggtttgttgt tgcatggtgt taacttggct      600

accattttgt gttttccagc tgctgttgca tttctgttgg aatctattac tccagctgtt      660

ttgttgccag gtagacaacc attggttcca agagcacaag gttggggtca aggtcaaggt      720

tgttttggta gatctttgtt gttaccattg aggccagatc ctgttttgag agcacaattg      780

ccacctttac caccacatcc aaaggctttg cctgctgctg ctactccagg tgatgctgtt      840

ccacatccag ctccaggtgg tgcagatcct gcagttgatg gtcctggtca tccagaattg      900

catgaagcct tacaaggtcc acaaccacct catttggctc acttgttgtt gttagctttg      960

ccattattgc cagagagaag aggtagagca catgctgttt ggaggccaag agttttgcca     1020

ggtttggttg aattgagagt ttaccatttg ttgctggccg aattggaaca tccatgttct     1080

caagttgttc accaaacttt gttgcaagct catgctccag ctggtcaaca acaggttggt     1140

tctcaagatg gttctatttc cggtttgaga ttgctaccaa gagttccagg tgaacatcca     1200

ccagctcatg ttccaccatt gggtttacat ggtcatgatg gtgctgaccc agctggttta     1260

gattctggtc cattattacc aagacagttg agacagagat ctagagttgc tgttactcat     1320

cattgggctg ccggtggtag accagatgtt agaccaagat tattgagagc ccaaccataa     1380


<210>  29
<211>  489
<212>  PRT
<213>  Bos taurus

<400>  29

Met Gly Asp Arg Gly Gly Ala Gly Gly Ser Arg Arg Arg Arg Thr Gly 
1               5                   10                  15      


Ser Arg Pro Ser Ile Gln Gly Gly Ser Gly Pro Ala Ala Ala Glu Glu 
            20                  25                  30          


Glu Val Arg Asp Val Gly Ala Gly Gly Asp Ala Pro Val Arg Asp Thr 
        35                  40                  45              


Asp Lys Asp Gly Asp Val Asp Val Gly Ser Gly His Trp Asp Leu Arg 
    50                  55                  60                  


Cys His Arg Leu Gln Asp Ser Leu Phe Ser Ser Asp Ser Gly Phe Ser 
65                  70                  75                  80  


Asn Tyr Arg Gly Ile Leu Asn Trp Cys Val Val Met Leu Ile Leu Ser 
                85                  90                  95      


Asn Ala Arg Leu Phe Leu Glu Asn Leu Ile Lys Tyr Gly Ile Leu Val 
            100                 105                 110         


Asp Pro Ile Gln Val Val Ser Leu Phe Leu Lys Asp Pro Tyr Ser Trp 
        115                 120                 125             


Pro Ala Leu Cys Leu Val Ile Val Ala Asn Ile Phe Ala Val Ala Ala 
    130                 135                 140                 


Phe Gln Val Glu Lys Arg Leu Ala Val Gly Ala Leu Thr Glu Gln Ala 
145                 150                 155                 160 


Gly Leu Leu Leu His Gly Val Asn Leu Ala Thr Ile Leu Cys Phe Pro 
                165                 170                 175     


Ala Ala Val Ala Phe Leu Leu Glu Ser Ile Thr Pro Val Gly Ser Val 
            180                 185                 190         


Leu Ala Leu Met Val Tyr Thr Ile Leu Phe Leu Lys Leu Phe Ser Tyr 
        195                 200                 205             


Arg Asp Val Asn Leu Trp Cys Arg Glu Arg Arg Ala Gly Ala Lys Ala 
    210                 215                 220                 


Lys Ala Ala Leu Ala Gly Lys Ala Ala Asn Gly Gly Ala Ala Gln Arg 
225                 230                 235                 240 


Thr Val Ser Tyr Pro Asp Asn Leu Thr Tyr Arg Asp Leu Tyr Tyr Phe 
                245                 250                 255     


Leu Phe Ala Pro Thr Leu Cys Tyr Glu Leu Asn Phe Pro Arg Ser Pro 
            260                 265                 270         


Arg Ile Arg Lys Arg Phe Leu Leu Arg Arg Leu Leu Glu Met Leu Phe 
        275                 280                 285             


Leu Thr Gln Leu Gln Val Gly Leu Ile Gln Gln Trp Met Val Pro Ala 
    290                 295                 300                 


Ile Gln Asn Ser Met Lys Pro Phe Lys Asp Met Asp Tyr Ser Arg Ile 
305                 310                 315                 320 


Val Glu Arg Leu Leu Lys Leu Ala Val Pro Asn His Leu Ile Trp Leu 
                325                 330                 335     


Ile Phe Phe Tyr Trp Leu Phe His Ser Cys Leu Asn Ala Val Ala Glu 
            340                 345                 350         


Leu Met Gln Phe Gly Asp Arg Glu Phe Tyr Arg Asp Trp Trp Asn Ser 
        355                 360                 365             


Glu Ser Ile Thr Tyr Phe Trp Gln Asn Trp Asn Ile Pro Val His Lys 
    370                 375                 380                 


Trp Cys Ile Arg His Phe Tyr Lys Pro Met Leu Arg Arg Gly Ser Ser 
385                 390                 395                 400 


Lys Trp Ala Ala Arg Thr Ala Val Phe Leu Ala Ser Ala Phe Phe His 
                405                 410                 415     


Glu Tyr Leu Val Ser Ile Pro Leu Arg Met Phe Arg Leu Trp Ala Phe 
            420                 425                 430         


Thr Gly Met Met Ala Gln Ile Pro Leu Ala Trp Ile Val Gly Arg Phe 
        435                 440                 445             


Phe Arg Gly Asn Tyr Gly Asn Ala Ala Val Trp Leu Ser Leu Ile Ile 
    450                 455                 460                 


Gly Gln Pro Val Ala Val Leu Met Tyr Val His Asp Tyr Tyr Val Leu 
465                 470                 475                 480 


Asn Arg Glu Ala Pro Ala Ala Gly Thr 
                485                 


<210>  30
<211>  1470
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of the protein coding region of Bos taurus 
       (cattle) diacylglycerol O-acyltransferase 1 lactation isoform X1 
       (BtDGAT1lac) in pAT019, codon optimised for expression in yeast.

<400>  30
atgggtgata gaggtggtgc tggtggttct agaagaagaa ggacaggttc tagaccatct       60

attcaaggtg gttctggtcc agctgctgct gaagaagagg ttagagatgt tggtgccggt      120

ggtgatgctc cagttagaga tactgataag gatggtgatg ttgatgttgg ttcaggtcat      180

tgggatttga gatgtcatag attgcaggac agcttgttct cttctgattc tggtttttct      240

aactacaggg gtattttgaa ctggtgcgtt gttatgttga ttttgtctaa cgctaggctg      300

ttcttggaga acttgattaa gtacggtatc ttggttgatc caatccaggt tgtttctttg      360

ttcttgaagg atccatattc ttggccagct ttgtgtttgg ttatcgttgc taacattttt      420

gctgttgctg ccttccaagt cgaaaaaaga ttggctgttg gtgctttgac tgaacaagca      480

ggtttgttgt tgcatggtgt taacttggct accattttgt gttttccagc tgcagttgct      540

ttcttgttgg aatctattac tccagttggt tccgttttgg ctttgatggt ttacactatc      600

ctgttcctaa agctgttctc ctacagagat gttaatttgt ggtgtagaga aagacgtgct      660

ggtgctaaag ctaaagctgc tttggctggt aaagctgcaa atggtggtgc agctcaaaga      720

actgtttctt acccagataa cttgacttac agggacttgt actacttttt gttcgctcca      780

actttgtgct acgaattgaa ttttccaaga agcccaagga tcagaaagag gtttttgttg      840

agaaggttgt tggagatgtt gtttttgacc caattgcaag tcggtttgat ccaacaatgg      900

atggttccag ctattcagaa ttctatgaag ccattcaagg acatggacta ctctagaatc      960

gtcgaaagat tattgaagtt ggccgttcca aaccatttga tctggttgat ttttttctac     1020

tggctgttcc actcttgctt gaatgctgtt gcagaattga tgcaatttgg tgacagagaa     1080

ttttacagag actggtggaa ctctgaatcc attacttact tttggcagaa ctggaacatc     1140

ccagttcata agtggtgtat cagacatttc tacaagccaa tgttgcgtag aggttcttct     1200

aaatgggctg ctagaactgc tgtttttttg gcttctgctt tcttccacga atacttggtt     1260

tctatcccat tgagaatgtt cagattgtgg gcttttactg gtatgatggc tcaaattcca     1320

ttagcttgga tcgttggtag attcttcaga ggtaattatg gtaatgctgc cgtttggttg     1380

tccttgatta ttggtcaacc agttgctgtt ttgatgtacg ttcatgatta ctacgtcttg     1440

aacagagaag ctcctgctgc tggtacttaa                                      1470


