                         SEQUENCE LISTING

<110>  University of Maryland, Baltimore
 
<120>  LIVE SALMONELLA TYPHI VECTORS ENGINEERED TO EXPRESS CANCER 
       PROTEIN ANTIGENS AND METHODS OF USE THEREOF

<130>  UMB-057PCT1

<150>  62/985,699
<151>  2020-03-05

<160>  31    

<170>  PatentIn version 3.5

<210>  1
<211>  650
<212>  DNA
<213>  Artificial sequence

<220>
<223>  pagL

<400>  1
ggatccaatt gaaaataagg aggaaaaaat gactagtatg aagcgcatct ttatttatct       60

gttacttccg tgtgcattcg catgttctgc taatgataat gttttttttg gcaagggcaa      120

caagcatcag atctcttttg ctgcgggaga aagtataaga agaggagggg ttgagcactt      180

atatacggct tttctgacat acagtgaacc cagcgatttt ttctttttac aggcaagaaa      240

taatctggag ttaggaggat ttaaggctaa gggtagcgat gattgcagta aacattctgg      300

cagcgttccc tgtaataaat ataaccaggg cgtattgggt atctcgaagg atgtggcgct      360

ggttcatttc gctggtatct ataccggtat tggtctgggg gcttatataa aatctaagtc      420

gcgagatgat atgcgtgtca attctgcatt tacctttgga gaaaaagcgt ttcttggctg      480

gaactttggg gctttttcta cagaagctta tatccggcat ttctcgaatg gatcacttac      540

ggataaaaat tcagggcata attttgtagg tgcttcaatt agttataatt tctgataata      600

gttgataacc taggccgcct aatgagcggg cttttttttc tcgggctagc                 650


<210>  2
<211>  188
<212>  PRT
<213>  Salmonella enterica

<400>  2

Met Thr Ser Met Lys Arg Ile Phe Ile Tyr Leu Leu Leu Pro Cys Ala 
1               5                   10                  15      


Phe Ala Cys Ser Ala Asn Asp Asn Val Phe Phe Gly Lys Gly Asn Lys 
            20                  25                  30          


His Gln Ile Ser Phe Ala Ala Gly Glu Ser Ile Arg Arg Gly Gly Val 
        35                  40                  45              


Glu His Leu Tyr Thr Ala Phe Leu Thr Tyr Ser Glu Pro Ser Asp Phe 
    50                  55                  60                  


Phe Phe Leu Gln Ala Arg Asn Asn Leu Glu Leu Gly Gly Phe Lys Ala 
65                  70                  75                  80  


Lys Gly Ser Asp Asp Cys Ser Lys His Ser Gly Ser Val Pro Cys Asn 
                85                  90                  95      


Lys Tyr Asn Gln Gly Val Leu Gly Ile Ser Lys Asp Val Ala Leu Val 
            100                 105                 110         


His Phe Ala Gly Ile Tyr Thr Gly Ile Gly Leu Gly Ala Tyr Ile Lys 
        115                 120                 125             


Ser Lys Ser Arg Asp Asp Met Arg Val Asn Ser Ala Phe Thr Phe Gly 
    130                 135                 140                 


Glu Lys Ala Phe Leu Gly Trp Asn Phe Gly Ala Phe Ser Thr Glu Ala 
145                 150                 155                 160 


Tyr Ile Arg His Phe Ser Asn Gly Ser Leu Thr Asp Lys Asn Ser Gly 
                165                 170                 175     


His Asn Phe Val Gly Ala Ser Ile Ser Tyr Asn Phe 
            180                 185             


<210>  3
<211>  647
<212>  DNA
<213>  Artificial sequence

<220>
<223>  pagL

<400>  3
ggatccaatt gaaaataagg aggaaaaagt gtatatgaag cgcatcttta tttatctgtt       60

acttccgtgt gcattcgcat gttctgctaa tgataatgtt ttttttggca agggcaacaa      120

gcatcagatc tcttttgctg cgggagaaag tataagaaga ggaggggttg agcacttata      180

tacggctttt ctgacataca gtgaacccag cgattttttc tttttacagg caagaaataa      240

tctggagtta ggaggattta aggctaaggg tagcgatgat tgcagtaaac attctggcag      300

cgttccctgt aataaatata accagggcgt attgggtatc tcgaaggatg tggcgctggt      360

tcatttcgct ggtatctata ccggtattgg tctgggggct tatataaaat ctaagtcgcg      420

agatgatatg cgtgtcaatt ctgcatttac ctttggagaa aaagcgtttc ttggctggaa      480

ctttggggct ttttctacag aagcttatat ccggcatttc tcgaatggat cacttacgga      540

taaaaattca gggcataatt ttgtaggtgc ttcaattagt tataatttct gataatagtt      600

gataacctag gccgcctaat gagcgggctt ttttttctcg ggctagc                    647


<210>  4
<211>  187
<212>  PRT
<213>  Salmonella enterica

<400>  4

Met Tyr Met Lys Arg Ile Phe Ile Tyr Leu Leu Leu Pro Cys Ala Phe 
1               5                   10                  15      


Ala Cys Ser Ala Asn Asp Asn Val Phe Phe Gly Lys Gly Asn Lys His 
            20                  25                  30          


Gln Ile Ser Phe Ala Ala Gly Glu Ser Ile Arg Arg Gly Gly Val Glu 
        35                  40                  45              


His Leu Tyr Thr Ala Phe Leu Thr Tyr Ser Glu Pro Ser Asp Phe Phe 
    50                  55                  60                  


Phe Leu Gln Ala Arg Asn Asn Leu Glu Leu Gly Gly Phe Lys Ala Lys 
65                  70                  75                  80  


Gly Ser Asp Asp Cys Ser Lys His Ser Gly Ser Val Pro Cys Asn Lys 
                85                  90                  95      


Tyr Asn Gln Gly Val Leu Gly Ile Ser Lys Asp Val Ala Leu Val His 
            100                 105                 110         


Phe Ala Gly Ile Tyr Thr Gly Ile Gly Leu Gly Ala Tyr Ile Lys Ser 
        115                 120                 125             


Lys Ser Arg Asp Asp Met Arg Val Asn Ser Ala Phe Thr Phe Gly Glu 
    130                 135                 140                 


Lys Ala Phe Leu Gly Trp Asn Phe Gly Ala Phe Ser Thr Glu Ala Tyr 
145                 150                 155                 160 


Ile Arg His Phe Ser Asn Gly Ser Leu Thr Asp Lys Asn Ser Gly His 
                165                 170                 175     


Asn Phe Val Gly Ala Ser Ile Ser Tyr Asn Phe 
            180                 185         


<210>  5
<211>  655
<212>  DNA
<213>  Artificial sequence

<220>
<223>  pagL

<400>  5
ggatccaatt gctattgaca ttgaaatggt ggtggaatgt atatgaagag aatatttata       60

tatctattat taccttgtgc attcgcatgt tctgctaatg ataatgtttt ttttggcaag      120

ggcaacaagc atcagatctc ttttgctgcg ggagaaagta taagaagagg aggggttgag      180

cacttatata cggcttttct gacatacagt gaacccagcg attttttctt tttacaggca      240

agaaataatc tggagttagg aggatttaag gctaagggta gcgatgattg cagtaaacat      300

tctggcagcg ttccctgtaa taaatataac cagggcgtat tgggtatctc gaaggatgtg      360

gcgctggttc atttcgctgg tatctatacc ggtattggtc tgggggctta tataaaatct      420

aagtcgcgag atgatatgcg tgtcaattct gcatttacct ttggagaaaa agcgtttctt      480

ggctggaact ttggggcttt ttctacagaa gcttatatcc ggcatttctc gaatggatca      540

cttacggata aaaattcagg gcataatttt gtaggtgctt caattagtta taatttctga      600

taatagttga taacctaggc cgcctaatga gcgggctttt ttttctcggg ctagc           655


<210>  6
<211>  6909
<212>  DNA
<213>  Artificial sequence

<220>
<223>  pagL

<400>  6
gaattctgtg gtagcacaga ataatgaaaa gtgtgtaaag aagggtaaaa aaaaccgaat       60

gcgaggcatc cggttgaaat aggggtaaac agacattcag aaatgaatga cggtaataaa      120

taaagttaat gatgatagcg ggagttattc tagttgcgag tgaaggtttt gttttgacat      180

tcagtgctgt caaatactta agaataagtt attgatttta accttgaatt attattgctt      240

gatgttaggt gcttatttcg ccattccgca ataatcttaa aaagttccct tgcatttaca      300

ttttgaaaca tctatagcga taaatgaaac atcttaaaag ttttagtatc atattcgtgt      360

tggattattc tgcatttttg gggagaatgg acttgccgac tgattaatga gggttaatca      420

gtatgcagtg gcataaaaaa gcaaataaag gcatataaca gatcgatctt aaacatccac      480

aggaggatgg gatccaattg aaaataagga ggaaaaaatg actagtatga agcgcatctt      540

tatttatctg ttacttccgt gtgcattcgc atgttctgct aatgataatg ttttttttgg      600

caagggcaac aagcatcaga tctcttttgc tgcgggagaa agtataagaa gaggaggggt      660

tgagcactta tatacggctt ttctgacata cagtgaaccc agcgattttt tctttttaca      720

ggcaagaaat aatctggagt taggaggatt taaggctaag ggtagcgatg attgcagtaa      780

acattctggc agcgttccct gtaataaata taaccagggc gtattgggta tctcgaagga      840

tgtggcgctg gttcatttcg ctggtatcta taccggtatt ggtctggggg cttatataaa      900

atctaagtcg cgagatgata tgcgtgtcaa ttctgcattt acctttggag aaaaagcgtt      960

tcttggctgg aactttgggg ctttttctac agaagcttat atccggcatt tctcgaatgg     1020

atcacttacg gataaaaatt cagggcataa ttttgtaggt gcttcaatta gttataattt     1080

ctgataatag ttgataacct aggccgccta atgagcgggc ttttttttct cgggctagct     1140

gataacctag cccgcctaat gagcgggctt ttttttctcg gcctaggttt cacctgttct     1200

attaggtgtt acatgctgtt catctgttac attgtcgatc tgttcatggt gaacagcttt     1260

aaatgcacca aaaactcgta aaagctctga tgtatctatc ttttttacac cgttttcatc     1320

tgtgcatatg gacagttttc cctttgatat ctaacggtga acagttgttc tacttttgtt     1380

tgttagtctt gatgcttcac tgatagatac aagagccata agaacctcag atccttccgt     1440

atttagccag tatgttctct agtgtggttc gttgtttttg cgtgagccat gagaacgaac     1500

cattgagatc atgcttactt tgcatgtcac tcaaaaattt tgcctcaaaa ctggtgagct     1560

gaatttttgc agttaaagca tcgtgtagtg tttttcttag tccgttacgt aggtaggaat     1620

ctgatgtaat ggttgttggt attttgtcac cattcatttt tatctggttg ttctcaagtt     1680

cggttacgag atccatttgt ctatctagtt caacttggaa aatcaacgta tcagtcgggc     1740

ggcctcgctt atcaaccacc aatttcatat tgctgtaagt gtttaaatct ttacttattg     1800

gtttcaaaac ccattggtta agccttttaa actcatggta gttattttca agcattaaca     1860

tgaacttaaa ttcatcaagg ctaatctcta tatttgcctt gtgagttttc ttttgtgtta     1920

gttcttttaa taaccactca taaatcctca tagagtattt gttttcaaaa gacttaacat     1980

gttccagatt atattttatg aattttttta actggaaaag ataaggcaat atctcttcac     2040

taaaaactaa ttctaatttt tcgcttgaga acttggcata gtttgtccac tggaaaatct     2100

caaagccttt aaccaaagga ttcctgattt ccacagttct cgtcatcagc tctctggttg     2160

ctttagctaa tacaccataa gcattttccc tactgatgtt catcatctga gcgtattggt     2220

tataagtgaa cgataccgtc cgttctttcc ttgtagggtt ttcaatcgtg gggttgagta     2280

gtgccacaca gcataaaatt agcttggttt catgctccgt taagtcatag cgactaatcg     2340

ctagttcatt tgctttgaaa acaactaatt cagacataca tctcaattgg tctaggtgat     2400

tttaatcact ataccaattg agatgggcta gtcaatgata attactagtc cttttccttt     2460

gagttgtggg tatctgtaaa ttctgctaga cctttgctgg aaaacttgta aattctgcta     2520

gaccctctgt aaattccgct agacctttgt gtgttttttt tgtttatatt caagtggtta     2580

taatttatag aataaagaaa gaataaaaaa agataaaaag aatagatccc agccctgtgt     2640

ataactcact actttagtca gttccgcagt attacaaaag gatgtcgcaa acgctgtttg     2700

ctcctctaca aaacagacct taaaacccta aaggcttaag tagcaccctc gcaagctcgg     2760

gcaaatcgct gaatattcct tttgtctccg accatcaggc acctgagtcg ctgtcttttt     2820

cgtgacattc agttcgctgc gctcacggct ctggcagtga atgggggtaa atggcactac     2880

aggcgccttt tatggattca tgcaaggaaa ctacccataa tacaagaaaa gcccgtcacg     2940

ggcttctcag ggcgttttat ggcgggtctg ctatgtggtg ctatctgact ttttgctgtt     3000

cagcagttcc tgccctctga ttttccagtc tgaccacttc ggattatccc gtgacaggtc     3060

attcagactg gctaatgcac ccagtaaggc agcggtatca tcaacaggct tacccgtctt     3120

actgtcaacc ggatctaaaa cactaggccc aagagtttgt agaaacgcaa aaaggccatc     3180

cgtcaggatg gccttctgct taatttgatg cctggcagtt tatggcgggc gtcctgcccg     3240

ccaccctccg ggccgttgct tcgcaacgtt caaatccgct cccggcggat ttgtcctact     3300

caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc     3360

ctttcgtttt atttgatgcc tggcagttcc ctactctcgc atggggagac cccacactac     3420

catcggcgct acggcgtttc acttctgagt tcggcatggg gtcaggtggg accaccgcgc     3480

tactgccgcc aggcaaattc tgttttatca gaccgcttct gcgttctgat ttaatctgta     3540

tcaggctgaa aatcttctct catccgccaa aacagccaag ctggatctaa aacactagcc     3600

caacctttca tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc     3660

ttggtcggtc atttcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag     3720

aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc     3780

cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg     3840

tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg     3900

atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgcgc     3960

gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca     4020

tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct     4080

tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc     4140

atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact     4200

tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa     4260

ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctgcag ttcattcagg     4320

gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac     4380

acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc     4440

acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct     4500

catcctgtct cttgatcaga tcttgatccc ctgcgccatc agatccttgg cggcaagaaa     4560

gccatccagt ttactttgca gggcttccca accttaccag agggcgcccc agccgtggca     4620

attccggttc gctgctagac aacatcagca aggagaaagg ggctaccggc gaaccagcag     4680

cccctttata aaggcgcttc agtagtcaga ccagcatcag tcctgaaaag gcgggcctgc     4740

gcccgcctcc aggttgctac ttaccggatt cgtaagccat gaaagccgcc acctccctgt     4800

gtccgtctct gtaacgaatc tcgcacagcg attttcgtgt cagataagtg aatatcaaca     4860

gtgtgagaca cacgatcaac acacaccaga caagggaact tcgtggtagt ttcatggcct     4920

tcttctcctt gcgcaaagcg cggtaagagg ctatcctgat gtggactaga catagggatg     4980

cctcgtggtg gttaatgaaa attaacttac tacggggcta tcttctttct gccacacaac     5040

acggcaacaa accaccttca cgtcatgagg cagaaagcct caagcgccgg gcacatcata     5100

gcccatatac ctgcacgctg accacactca ctttccctga aaataatccg ctcattcaga     5160

ccgttcacgg gaaatccgtg tgattgttgc cgcatcacgc tgcctcccgg agtttgtctc     5220

gagcactttt gttacccgcc aaacaaaacc caaaaacaac ccatacccaa cccaataaaa     5280

caccaaaaca agacaaataa tcattgattg atggttgaaa tggggtaaac ttgacaaaca     5340

aacccactta aaacccaaaa catacccaaa cacacaccaa aaaaacacca taaggagttt     5400

tataaatgtt ggtattcatt gatgacggtt caacaaacat caaactacag tggcaggaaa     5460

gcgacggaac aattaaacag cacattagcc cgaacagctt caaacgcgag tgggcagtct     5520

cttttggtga taaaaaggtc tttaactaca cactgaacgg cgaacagtat tcatttgatc     5580

caatcagccc ggatgctgta gtcacaacca atatcgcatg gcaatacagc gacgttaatg     5640

tcgttgcagt gcatcacgcc ttactgacca gtggtctgcc ggtaagcgaa gtggatattg     5700

tttgcacact tcctctgaca gagtattacg acagaaataa ccaacccaat acggaaaata     5760

ttgagcgtaa gaaagcaaac ttccggaaaa aaattacatt aaatggcggg gatacattca     5820

caataaaaga tgtaaaagtc atgcctgaat ctataccggc aggttatgaa gttctacaag     5880

aactggatga gttagattct ttattaatta tagatctcgg gggcaccaca ttagatattt     5940

ctcaggtaat ggggaaatta tcggggatca gtaaaatata cggagactca tctcttggtg     6000

tctctctggt tacatctgca gtaaaagatg ccctttctct tgcgagaaca aaaggaagta     6060

gctatcttgc tgacgatata atcattcaca gaaaagataa taactatctg aagcaacgaa     6120

ttaatgatga gaacaaaata tcaatagtca ccgaagcaat gaatgaagca cttcgtaaac     6180

ttgagcaacg tgtattaaat acgctcaatg aattttctgg ttatactcat gttatggtta     6240

taggcggtgg cgcagaatta atatgcgatg cagtaaaaaa acacacacag attcgtgatg     6300

aacgtttttt caaaaccaat aactctcaat atgatttagt taacggtatg tatctcatag     6360

gtaattaatg atggacaagc gcagaaccat tgccttcaaa ctaaatccag atgtaaatca     6420

aacagataaa attgtttgtg atacactgga cagtatcccg caaggggaac gaagccgcct     6480

taaccgggcc gcactgacgg caggtctggc cttatacaga caagatcccc ggaccccttt     6540

ccttttatgt gagctgctga cgaaagaaac cacattttca gatatcgtga atatattgag     6600

atcgctattt ccaaaagaga tggccgattt taattcttca atagtcactc aatcctcttc     6660

acaacaagag caaaaaagtg atgaagagac caaaaaaaat gcgatgaagc taataaatta     6720

attcaattat tattgagttc cctttatcca ctatcaggct ggataaaggg aactcaatca     6780

agttattttc ttaccagtca ttacataatc gttattatga aataatcgtt tgcactgtct     6840

ctgttattca ggcaatttca ataaaggcac ttgctcacgc tctgtcattt tctgaaactc     6900

ttcatgctg                                                             6909


<210>  7
<211>  3080
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PompC-bamA (in pSEC10 vector)

<400>  7
gaattctgtg gtagcacaga ataatgaaaa gtgtgtaaag aagggtaaaa aaaaccgaat       60

gcgaggcatc cggttgaaat aggggtaaac agacattcag aaatgaatga cggtaataaa      120

taaagttaat gatgatagcg ggagttattc tagttgcgag tgaaggtttt gttttgacat      180

tcagtgctgt caaatactta agaataagtt attgatttta accttgaatt attattgctt      240

gatgttaggt gcttatttcg ccattccgca ataatcttaa aaagttccct tgcatttaca      300

ttttgaaaca tctatagcga taaatgaaac atcttaaaag ttttagtatc atattcgtgt      360

tggattattc tgcatttttg gggagaatgg acttgccgac tgattaatga gggttaatca      420

gtatgcagtg gcataaaaaa gcaaataaag gcatataaca gatcgatctt aaacatccac      480

aggaggatgg gatccgctag caaaataagg aggaaaaagt gcggcacaca cattttttaa      540

tgcctttggc acttgttagc gctatggcag cggtacaaca agcatatgca gctgatgatt      600

tcgttgttag agatattcgt gtaaacgggt tggtccgtct tacccctgca aatgtttata      660

ccatgttgcc aatcaacagt ggcgatcgcg ttaatgaacc gatgatcgcc gaagcaattc      720

gaacattgta tgccactgga ctttttgatg atattaaagc atcaaaagaa aatgatactt      780

tggtttttaa tgttattgag cgcccaatta tttcaaagct tgaatttaag ggtaataaac      840

ttattcctaa agaggctcta gaacaaggcc tcaaaaaaat gggtattgct gagggtgagg      900

tttttaagaa atctgctttg caaaccattg agacagaatt agagcaacaa tatactcaac      960

aaggccgtta tgatgctgat gttacagttg atacagttgc tcgcccaaat aaccgcgttg     1020

aattaaaaat taatttcaat gaaggaacgc cagcaaaagt ctttgatatt aatgtaattg     1080

gtaatactgt ttttaaagac agtgaaatta aacaagcatt tgcagttaaa gaaagtggtt     1140

gggcttcagt agtcacacgt aatgaccgct atgcacgtga aaaaatggct gcaagccttg     1200

aagctttacg tgccatgtac cttaacaaag gctatattaa ttttaatatt aataattccc     1260

aactcaatat tagtgaagat aaaaagcata tctttattga ggttgctgtt gatgaaggta     1320

gccagtttaa atttggtcaa actaaattct taggtgatgc gctttataag cctgaagaat     1380

tacaagcttt aaaaatttat aaagacggtg atacctattc acaagaaaaa gtaaatgcgg     1440

ttaagcaatt acttttacgt aagtatggta atgcaggtta ttactttgca gatgtaaata     1500

ttgtgccgca gattaataat gaaactggcg ttgttgactt aaattattat gtgaatccag     1560

gtcaacaagt taccgtacgt cgtattaatt ttactggtaa tagcaaaact tctgatgaag     1620

tattacgtcg tgaaatgcgt cagatggaag gtgcattagc gagcaatgaa aaaattgact     1680

tatctaaagt tcgtttagag cgtacaggtt tctttaaaac cgttgatatt aaacctgctc     1740

gtattccaaa ctctccagac caagttgact taaacgtaaa tgttgaagaa caacattcag     1800

gtactactac attagcagtt ggttattcgc aaagtggtgg tattacgttc caagcaggac     1860

ttagtcaaac taactttatg ggtacaggta accgagttgc aattgactta tctcgttctg     1920

aaactcaaga ctattacaac ttaagtgtta ctgaccctta ctttacaatt gatggtgtaa     1980

gccgcggtta taatgtctat tatcgtaaaa cgaaactgaa tgatgactat aacgttaaca     2040

actatgtaac ggatagtttt ggtggtagtt taagttttgg ttaccctatt gatgaaaatc     2100

aaagtttaag tgcttcggtg ggtgttgata atactaaggt aacaacaggt gcatttgtat     2160

ccacttatgt acgtgattac ttattggcaa atggtgggaa aactacttct acaaatacgt     2220

actgcctcgt tgatcttgtt caagatcccc aaacaggatt atataaatgc ccagaaggtc     2280

aaacttctca accttatgga aatgcgtttg aaggagagtt ctttacttat aacctgaatt     2340

tgggttggtc gtacaatact ttgaatagac caatctttcc aacatcaggc atgtctcacc     2400

gtgtaggtct tgaaattggt ttaccgggta gtgatgttga ttatcaaaaa gtaacttatg     2460

atactcaagc atttttccct attggaagta ccggctttgt acttcgtggt tatggtaagc     2520

ttgggtatgg taatgattta ccattctata agaacttcta tgcaggtggt tatggctctg     2580

tacgtggtta tgataacagt actttagggc ctaaatatgc cagtgtaaat ctacaagaag     2640

agaaaaaaaa tgactcttct ccagaagaag tgggtggtaa tgctctagtg cagttcggca     2700

cagaattggt tttaccaatg ccatttaaag gtgactggac tcgccaagta cgccctgttc     2760

tatttgctga aggtggtcaa gtatttgata ccaagtgcga cgttcgatct tattctatga     2820

ttatgaatgg tcagcaaatt tctgatgcta aaaaatactg tgaagataat tatggatttg     2880

atttaggtaa cttgcgttat agtgtgggtg tcggtgtaac ctggattact atgattggtc     2940

cattgtcact tagctatgca ttcccgttaa atgataaacc gggtgatgaa actaaagaaa     3000

ttcagtttga aattggtcgt actttctgat aatagatctg cacgccgcct aatgagcggg     3060

cttttttttc tcggcctagg                                                 3080


<210>  8
<211>  836
<212>  PRT
<213>  Acinetobacter baumannii

<400>  8

Met Arg His Thr His Phe Leu Met Pro Leu Ala Leu Val Ser Ala Met 
1               5                   10                  15      


Ala Ala Val Gln Gln Ala Tyr Ala Ala Asp Asp Phe Val Val Arg Asp 
            20                  25                  30          


Ile Arg Val Asn Gly Leu Val Arg Leu Thr Pro Ala Asn Val Tyr Thr 
        35                  40                  45              


Met Leu Pro Ile Asn Ser Gly Asp Arg Val Asn Glu Pro Met Ile Ala 
    50                  55                  60                  


Glu Ala Ile Arg Thr Leu Tyr Ala Thr Gly Leu Phe Asp Asp Ile Lys 
65                  70                  75                  80  


Ala Ser Lys Glu Asn Asp Thr Leu Val Phe Asn Val Ile Glu Arg Pro 
                85                  90                  95      


Ile Ile Ser Lys Leu Glu Phe Lys Gly Asn Lys Leu Ile Pro Lys Glu 
            100                 105                 110         


Ala Leu Glu Gln Gly Leu Lys Lys Met Gly Ile Ala Glu Gly Glu Val 
        115                 120                 125             


Phe Lys Lys Ser Ala Leu Gln Thr Ile Glu Thr Glu Leu Glu Gln Gln 
    130                 135                 140                 


Tyr Thr Gln Gln Gly Arg Tyr Asp Ala Asp Val Thr Val Asp Thr Val 
145                 150                 155                 160 


Ala Arg Pro Asn Asn Arg Val Glu Leu Lys Ile Asn Phe Asn Glu Gly 
                165                 170                 175     


Thr Pro Ala Lys Val Phe Asp Ile Asn Val Ile Gly Asn Thr Val Phe 
            180                 185                 190         


Lys Asp Ser Glu Ile Lys Gln Ala Phe Ala Val Lys Glu Ser Gly Trp 
        195                 200                 205             


Ala Ser Val Val Thr Arg Asn Asp Arg Tyr Ala Arg Glu Lys Met Ala 
    210                 215                 220                 


Ala Ser Leu Glu Ala Leu Arg Ala Met Tyr Leu Asn Lys Gly Tyr Ile 
225                 230                 235                 240 


Asn Phe Asn Ile Asn Asn Ser Gln Leu Asn Ile Ser Glu Asp Lys Lys 
                245                 250                 255     


His Ile Phe Ile Glu Val Ala Val Asp Glu Gly Ser Gln Phe Lys Phe 
            260                 265                 270         


Gly Gln Thr Lys Phe Leu Gly Asp Ala Leu Tyr Lys Pro Glu Glu Leu 
        275                 280                 285             


Gln Ala Leu Lys Ile Tyr Lys Asp Gly Asp Thr Tyr Ser Gln Glu Lys 
    290                 295                 300                 


Val Asn Ala Val Lys Gln Leu Leu Leu Arg Lys Tyr Gly Asn Ala Gly 
305                 310                 315                 320 


Tyr Tyr Phe Ala Asp Val Asn Ile Val Pro Gln Ile Asn Asn Glu Thr 
                325                 330                 335     


Gly Val Val Asp Leu Asn Tyr Tyr Val Asn Pro Gly Gln Gln Val Thr 
            340                 345                 350         


Val Arg Arg Ile Asn Phe Thr Gly Asn Ser Lys Thr Ser Asp Glu Val 
        355                 360                 365             


Leu Arg Arg Glu Met Arg Gln Met Glu Gly Ala Leu Ala Ser Asn Glu 
    370                 375                 380                 


Lys Ile Asp Leu Ser Lys Val Arg Leu Glu Arg Thr Gly Phe Phe Lys 
385                 390                 395                 400 


Thr Val Asp Ile Lys Pro Ala Arg Ile Pro Asn Ser Pro Asp Gln Val 
                405                 410                 415     


Asp Leu Asn Val Asn Val Glu Glu Gln His Ser Gly Thr Thr Thr Leu 
            420                 425                 430         


Ala Val Gly Tyr Ser Gln Ser Gly Gly Ile Thr Phe Gln Ala Gly Leu 
        435                 440                 445             


Ser Gln Thr Asn Phe Met Gly Thr Gly Asn Arg Val Ala Ile Asp Leu 
    450                 455                 460                 


Ser Arg Ser Glu Thr Gln Asp Tyr Tyr Asn Leu Ser Val Thr Asp Pro 
465                 470                 475                 480 


Tyr Phe Thr Ile Asp Gly Val Ser Arg Gly Tyr Asn Val Tyr Tyr Arg 
                485                 490                 495     


Lys Thr Lys Leu Asn Asp Asp Tyr Asn Val Asn Asn Tyr Val Thr Asp 
            500                 505                 510         


Ser Phe Gly Gly Ser Leu Ser Phe Gly Tyr Pro Ile Asp Glu Asn Gln 
        515                 520                 525             


Ser Leu Ser Ala Ser Val Gly Val Asp Asn Thr Lys Val Thr Thr Gly 
    530                 535                 540                 


Ala Phe Val Ser Thr Tyr Val Arg Asp Tyr Leu Leu Ala Asn Gly Gly 
545                 550                 555                 560 


Lys Thr Thr Ser Thr Asn Thr Tyr Cys Leu Val Asp Leu Val Gln Asp 
                565                 570                 575     


Pro Gln Thr Gly Leu Tyr Lys Cys Pro Glu Gly Gln Thr Ser Gln Pro 
            580                 585                 590         


Tyr Gly Asn Ala Phe Glu Gly Glu Phe Phe Thr Tyr Asn Leu Asn Leu 
        595                 600                 605             


Gly Trp Ser Tyr Asn Thr Leu Asn Arg Pro Ile Phe Pro Thr Ser Gly 
    610                 615                 620                 


Met Ser His Arg Val Gly Leu Glu Ile Gly Leu Pro Gly Ser Asp Val 
625                 630                 635                 640 


Asp Tyr Gln Lys Val Thr Tyr Asp Thr Gln Ala Phe Phe Pro Ile Gly 
                645                 650                 655     


Ser Thr Gly Phe Val Leu Arg Gly Tyr Gly Lys Leu Gly Tyr Gly Asn 
            660                 665                 670         


Asp Leu Pro Phe Tyr Lys Asn Phe Tyr Ala Gly Gly Tyr Gly Ser Val 
        675                 680                 685             


Arg Gly Tyr Asp Asn Ser Thr Leu Gly Pro Lys Tyr Ala Ser Val Asn 
    690                 695                 700                 


Leu Gln Glu Glu Lys Lys Asn Asp Ser Ser Pro Glu Glu Val Gly Gly 
705                 710                 715                 720 


Asn Ala Leu Val Gln Phe Gly Thr Glu Leu Val Leu Pro Met Pro Phe 
                725                 730                 735     


Lys Gly Asp Trp Thr Arg Gln Val Arg Pro Val Leu Phe Ala Glu Gly 
            740                 745                 750         


Gly Gln Val Phe Asp Thr Lys Cys Asp Val Arg Ser Tyr Ser Met Ile 
        755                 760                 765             


Met Asn Gly Gln Gln Ile Ser Asp Ala Lys Lys Tyr Cys Glu Asp Asn 
    770                 775                 780                 


Tyr Gly Phe Asp Leu Gly Asn Leu Arg Tyr Ser Val Gly Val Gly Val 
785                 790                 795                 800 


Thr Trp Ile Thr Met Ile Gly Pro Leu Ser Leu Ser Tyr Ala Phe Pro 
                805                 810                 815     


Leu Asn Asp Lys Pro Gly Asp Glu Thr Lys Glu Ile Gln Phe Glu Ile 
            820                 825                 830         


Gly Arg Thr Phe 
        835     


<210>  9
<211>  495
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PompC osmotically controlled promoter

<400>  9
gaattctgtg gtagcacaga ataatgaaaa gtgtgtaaag aagggtaaaa aaaaccgaat       60

gcgaggcatc cggttgaaat aggggtaaac agacattcag aaatgaatga cggtaataaa      120

taaagttaat gatgatagcg ggagttattc tagttgcgag tgaaggtttt gttttgacat      180

tcagtgctgt caaatactta agaataagtt attgatttta accttgaatt attattgctt      240

gatgttaggt gcttatttcg ccattccgca ataatcttaa aaagttccct tgcatttaca      300

ttttgaaaca tctatagcga taaatgaaac atcttaaaag ttttagtatc atattcgtgt      360

tggattattc tgcatttttg gggagaatgg acttgccgac tgattaatga gggttaatca      420

gtatgcagtg gcataaaaaa gcaaataaag gcatataaca gatcgatctt aaacatccac      480

aggaggatgg gatcc                                                       495


<210>  10
<211>  2517
<212>  DNA
<213>  Acinetobacter baumannii

<400>  10
gtgcggcaca cacatttttt aatgcctttg gcacttgtta gcgctatggc agcggtacaa       60

caagcatatg cagctgatga tttcgttgtt agagatattc gtgtaaacgg gttggtccgt      120

cttacccctg caaatgttta taccatgttg ccaatcaaca gtggcgatcg cgttaatgaa      180

ccgatgatcg ccgaagcaat tcgaacattg tatgccactg gactttttga tgatattaaa      240

gcatcaaaag aaaatgatac tttggttttt aatgttattg agcgcccaat tatttcaaag      300

cttgaattta agggtaataa acttattcct aaagaggctc tagaacaagg cctcaaaaaa      360

atgggtattg ctgagggtga ggtttttaag aaatctgctt tgcaaaccat tgagacagaa      420

ttagagcaac aatatactca acaaggccgt tatgatgctg atgttacagt tgatacagtt      480

gctcgcccaa ataaccgcgt tgaattaaaa attaatttca atgaaggaac gccagcaaaa      540

gtctttgata ttaatgtaat tggtaatact gtttttaaag acagtgaaat taaacaagca      600

tttgcagtta aagaaagtgg ttgggcttca gtagtcacac gtaatgaccg ctatgcacgt      660

gaaaaaatgg ctgcaagcct tgaagcttta cgtgccatgt accttaacaa aggctatatt      720

aattttaata ttaataattc ccaactcaat attagtgaag ataaaaagca tatctttatt      780

gaggttgctg ttgatgaagg tagccagttt aaatttggtc aaactaaatt cttaggtgat      840

gcgctttata agcctgaaga attacaagct ttaaaaattt ataaagacgg tgatacctat      900

tcacaagaaa aagtaaatgc ggttaagcaa ttacttttac gtaagtatgg taatgcaggt      960

tattactttg cagatgtaaa tattgtgccg cagattaata atgaaactgg cgttgttgac     1020

ttaaattatt atgtgaatcc aggtcaacaa gttaccgtac gtcgtattaa ttttactggt     1080

aatagcaaaa cttctgatga agtattacgt cgtgaaatgc gtcagatgga aggtgcatta     1140

gcgagcaatg aaaaaattga cttatctaaa gttcgtttag agcgtacagg tttctttaaa     1200

accgttgata ttaaacctgc tcgtattcca aactctccag accaagttga cttaaacgta     1260

aatgttgaag aacaacattc aggtactact acattagcag ttggttattc gcaaagtggt     1320

ggtattacgt tccaagcagg acttagtcaa actaacttta tgggtacagg taaccgagtt     1380

gcaattgact tatctcgttc tgaaactcaa gactattaca acttaagtgt tactgaccct     1440

tactttacaa ttgatggtgt aagccgcggt tataatgtct attatcgtaa aacgaaactg     1500

aatgatgact ataacgttaa caactatgta acggatagtt ttggtggtag tttaagtttt     1560

ggttacccta ttgatgaaaa tcaaagttta agtgcttcgg tgggtgttga taatactaag     1620

gtaacaacag gtgcatttgt atccacttat gtacgtgatt acttattggc aaatggtggg     1680

aaaactactt ctacaaatac gtactgcctc gttgatcttg ttcaagatcc ccaaacagga     1740

ttatataaat gcccagaagg tcaaacttct caaccttatg gaaatgcgtt tgaaggagag     1800

ttctttactt ataacctgaa tttgggttgg tcgtacaata ctttgaatag accaatcttt     1860

ccaacatcag gcatgtctca ccgtgtaggt cttgaaattg gtttaccggg tagtgatgtt     1920

gattatcaaa aagtaactta tgatactcaa gcatttttcc ctattggaag taccggcttt     1980

gtacttcgtg gttatggtaa gcttgggtat ggtaatgatt taccattcta taagaacttc     2040

tatgcaggtg gttatggctc tgtacgtggt tatgataaca gtactttagg gcctaaatat     2100

gccagtgtaa atctacaaga agagaaaaaa aatgactctt ctccagaaga agtgggtggt     2160

aatgctctag tgcagttcgg cacagaattg gttttaccaa tgccatttaa aggtgactgg     2220

actcgccaag tacgccctgt tctatttgct gaaggtggtc aagtatttga taccaagtgc     2280

gacgttcgat cttattctat gattatgaat ggtcagcaaa tttctgatgc taaaaaatac     2340

tgtgaagata attatggatt tgatttaggt aacttgcgtt atagtgtggg tgtcggtgta     2400

acctggatta ctatgattgg tccattgtca cttagctatg cattcccgtt aaatgataaa     2460

ccgggtgatg aaactaaaga aattcagttt gaaattggtc gtactttctg ataatag        2517


<210>  11
<211>  1594
<212>  DNA
<213>  Artificial sequence

<220>
<223>  LOA-linker-A3B3-linker-MUC1 ORF

<400>  11
ggatccaaaa taaggaggaa aaaaaatgaa agcgaccaaa ctggtgcttg gcgccgtgat       60

tctgggtagc acccttctgg ccggttgtag cagtaatgcg aaaatcgatc agggcattaa      120

cccgtatgtt ggctttgaaa tgggttacga ctggttaggt cgtatgccgt acaaaggcag      180

cgttgaaaac ggtgcataca aagctcaggg cgttcaactg accgctaaac tgggttaccc      240

gatcactgac gacctggaca tctacactcg tctgggtggc atggtatggc gtgcagacac      300

taaatccaac gtttatggta aaaaccacga caccggcgtt tctccggtct tcgctggcgg      360

tgttgagtac gcgatcactc ctgaaatcgc tacccgtctg gaataccagt ggaccaacaa      420

catcggtgac gcacacacca tcggcactcg tccggacaac ggcattccgg gtgcggaagc      480

cgctgcaaag gaggctgccg caaaagaagc agccgctaag gaggctgcgg caaaagcggc      540

tagcgtctct gcggagctgc cgaagccttc catctccagc aacaactcca aaccggtgga      600

ggacaaggat gctgtggcct tcacctgtga acctgaggct cagaacacaa cctacctttg      660

gtgggtaaat ggtcagagcc tgccggtcag tcctcgtctg cagctttcca atggcaaccg      720

caccctgact cttttcaatg tcacacgtaa tgacgcacgc gcctatgtat gtggaatcca      780

gaactcagtg agtgcaaacc gcagtgaccc tgtcaccctg gatgtccttt atgggccgga      840

caccccgatc atttcccctc cggactcgtc ttacctttcg ggagcggatt taaacctgtc      900

ctgtcactcg gcctctaacc cttccccgca gtattcttgg tttgtgaatg ggacctttca      960

gcaacacaca caagttattt ttatcaccaa aatcacgcct aataataacg ggacctatgc     1020

ctgttttgtc tctaacctgg ctactggccg caataattcc attgtcaaga gcatcacagt     1080

cgcggaagcc gctgcaaagg aggctgccgc aaaagaagca gccgctaagg aggctgcggc     1140

aaaagcgtct agaccggctc ctggtagcac ggcaccgcct gcgcatggtg taacttctgc     1200

tccggataat cgtcctgcgt taggaagtac tgctcctccg gctcacgggg tgacgagtgc     1260

acctgacacg cgcccggcac ctgggtctac agcgccgcct gcacatggag ttacatccgc     1320

gccggataat cgtcctgctt tgggatcgac tgcaccgcct gcccatgggg taaccagtgc     1380

ccctgatacc cgtccggcac ctggctcaac ggcccctccg gcacacggcg tcacgtcagc     1440

accggacaac cgtcctgctt tagggagcac agctccgcct gcgcatggag tcacttcagc     1500

gccggacaca cgccctgccc cgggctccac cgcccctccg gcccacggtg tcacctcggc     1560

ccctgacacc cgtcctaggt aatgatagac tagt                                 1594


<210>  12
<211>  518
<212>  PRT
<213>  Artificial sequence

<220>
<223>  LOA-linker-A3B3-linker-MUC1

<400>  12

Met Lys Ala Thr Lys Leu Val Leu Gly Ala Val Ile Leu Gly Ser Thr 
1               5                   10                  15      


Leu Leu Ala Gly Cys Ser Ser Asn Ala Lys Ile Asp Gln Gly Ile Asn 
            20                  25                  30          


Pro Tyr Val Gly Phe Glu Met Gly Tyr Asp Trp Leu Gly Arg Met Pro 
        35                  40                  45              


Tyr Lys Gly Ser Val Glu Asn Gly Ala Tyr Lys Ala Gln Gly Val Gln 
    50                  55                  60                  


Leu Thr Ala Lys Leu Gly Tyr Pro Ile Thr Asp Asp Leu Asp Ile Tyr 
65                  70                  75                  80  


Thr Arg Leu Gly Gly Met Val Trp Arg Ala Asp Thr Lys Ser Asn Val 
                85                  90                  95      


Tyr Gly Lys Asn His Asp Thr Gly Val Ser Pro Val Phe Ala Gly Gly 
            100                 105                 110         


Val Glu Tyr Ala Ile Thr Pro Glu Ile Ala Thr Arg Leu Glu Tyr Gln 
        115                 120                 125             


Trp Thr Asn Asn Ile Gly Asp Ala His Thr Ile Gly Thr Arg Pro Asp 
    130                 135                 140                 


Asn Gly Ile Pro Gly Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
145                 150                 155                 160 


Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Ala Ala Ser Val Ser Ala 
                165                 170                 175     


Glu Leu Pro Lys Pro Ser Ile Ser Ser Asn Asn Ser Lys Pro Val Glu 
            180                 185                 190         


Asp Lys Asp Ala Val Ala Phe Thr Cys Glu Pro Glu Ala Gln Asn Thr 
        195                 200                 205             


Thr Tyr Leu Trp Trp Val Asn Gly Gln Ser Leu Pro Val Ser Pro Arg 
    210                 215                 220                 


Leu Gln Leu Ser Asn Gly Asn Arg Thr Leu Thr Leu Phe Asn Val Thr 
225                 230                 235                 240 


Arg Asn Asp Ala Arg Ala Tyr Val Cys Gly Ile Gln Asn Ser Val Ser 
                245                 250                 255     


Ala Asn Arg Ser Asp Pro Val Thr Leu Asp Val Leu Tyr Gly Pro Asp 
            260                 265                 270         


Thr Pro Ile Ile Ser Pro Pro Asp Ser Ser Tyr Leu Ser Gly Ala Asp 
        275                 280                 285             


Leu Asn Leu Ser Cys His Ser Ala Ser Asn Pro Ser Pro Gln Tyr Ser 
    290                 295                 300                 


Trp Phe Val Asn Gly Thr Phe Gln Gln His Thr Gln Val Ile Phe Ile 
305                 310                 315                 320 


Thr Lys Ile Thr Pro Asn Asn Asn Gly Thr Tyr Ala Cys Phe Val Ser 
                325                 330                 335     


Asn Leu Ala Thr Gly Arg Asn Asn Ser Ile Val Lys Ser Ile Thr Val 
            340                 345                 350         


Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
        355                 360                 365             


Glu Ala Ala Ala Lys Ala Ser Arg Pro Ala Pro Gly Ser Thr Ala Pro 
    370                 375                 380                 


Pro Ala His Gly Val Thr Ser Ala Pro Asp Asn Arg Pro Ala Leu Gly 
385                 390                 395                 400 


Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr Arg 
                405                 410                 415     


Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala 
            420                 425                 430         


Pro Asp Asn Arg Pro Ala Leu Gly Ser Thr Ala Pro Pro Ala His Gly 
        435                 440                 445             


Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala Pro 
    450                 455                 460                 


Pro Ala His Gly Val Thr Ser Ala Pro Asp Asn Arg Pro Ala Leu Gly 
465                 470                 475                 480 


Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr Arg 
                485                 490                 495     


Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala 
            500                 505                 510         


Pro Asp Thr Arg Pro Arg 
        515             


<210>  13
<211>  1168
<212>  DNA
<213>  Artificial sequence

<220>
<223>  LOA-linker-A3B3-linker ORF

<400>  13
ggatccaaaa taaggaggaa aaaaaatgaa agcgaccaaa ctggtgcttg gcgccgtgat       60

tctgggtagc acccttctgg ccggttgtag cagtaatgcg aaaatcgatc agggcattaa      120

cccgtatgtt ggctttgaaa tgggttacga ctggttaggt cgtatgccgt acaaaggcag      180

cgttgaaaac ggtgcataca aagctcaggg cgttcaactg accgctaaac tgggttaccc      240

gatcactgac gacctggaca tctacactcg tctgggtggc atggtatggc gtgcagacac      300

taaatccaac gtttatggta aaaaccacga caccggcgtt tctccggtct tcgctggcgg      360

tgttgagtac gcgatcactc ctgaaatcgc tacccgtctg gaataccagt ggaccaacaa      420

catcggtgac gcacacacca tcggcactcg tccggacaac ggcattccgg gtgcggaagc      480

cgctgcaaag gaggctgccg caaaagaagc agccgctaag gaggctgcgg caaaagcggc      540

tagcgtctct gcggagctgc cgaagccttc catctccagc aacaactcca aaccggtgga      600

ggacaaggat gctgtggcct tcacctgtga acctgaggct cagaacacaa cctacctttg      660

gtgggtaaat ggtcagagcc tgccggtcag tcctcgtctg cagctttcca atggcaaccg      720

caccctgact cttttcaatg tcacacgtaa tgacgcacgc gcctatgtat gtggaatcca      780

gaactcagtg agtgcaaacc gcagtgaccc tgtcaccctg gatgtccttt atgggccgga      840

caccccgatc atttcccctc cggactcgtc ttacctttcg ggagcggatt taaacctgtc      900

ctgtcactcg gcctctaacc cttccccgca gtattcttgg tttgtgaatg ggacctttca      960

gcaacacaca caagttattt ttatcaccaa aatcacgcct aataataacg ggacctatgc     1020

ctgttttgtc tctaacctgg ctactggccg caataattcc attgtcaaga gcatcacagt     1080

cgcggaagcc gctgcaaagg aggctgccgc aaaagaagca gccgctaagg aggctgcggc     1140

aaaagcgtct aggtaatgat agactagt                                        1168


<210>  14
<211>  376
<212>  PRT
<213>  Artificial sequence

<220>
<223>  LOA-linker-A3B3-linker

<400>  14

Met Lys Ala Thr Lys Leu Val Leu Gly Ala Val Ile Leu Gly Ser Thr 
1               5                   10                  15      


Leu Leu Ala Gly Cys Ser Ser Asn Ala Lys Ile Asp Gln Gly Ile Asn 
            20                  25                  30          


Pro Tyr Val Gly Phe Glu Met Gly Tyr Asp Trp Leu Gly Arg Met Pro 
        35                  40                  45              


Tyr Lys Gly Ser Val Glu Asn Gly Ala Tyr Lys Ala Gln Gly Val Gln 
    50                  55                  60                  


Leu Thr Ala Lys Leu Gly Tyr Pro Ile Thr Asp Asp Leu Asp Ile Tyr 
65                  70                  75                  80  


Thr Arg Leu Gly Gly Met Val Trp Arg Ala Asp Thr Lys Ser Asn Val 
                85                  90                  95      


Tyr Gly Lys Asn His Asp Thr Gly Val Ser Pro Val Phe Ala Gly Gly 
            100                 105                 110         


Val Glu Tyr Ala Ile Thr Pro Glu Ile Ala Thr Arg Leu Glu Tyr Gln 
        115                 120                 125             


Trp Thr Asn Asn Ile Gly Asp Ala His Thr Ile Gly Thr Arg Pro Asp 
    130                 135                 140                 


Asn Gly Ile Pro Gly Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
145                 150                 155                 160 


Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Ala Ala Ser Val Ser Ala 
                165                 170                 175     


Glu Leu Pro Lys Pro Ser Ile Ser Ser Asn Asn Ser Lys Pro Val Glu 
            180                 185                 190         


Asp Lys Asp Ala Val Ala Phe Thr Cys Glu Pro Glu Ala Gln Asn Thr 
        195                 200                 205             


Thr Tyr Leu Trp Trp Val Asn Gly Gln Ser Leu Pro Val Ser Pro Arg 
    210                 215                 220                 


Leu Gln Leu Ser Asn Gly Asn Arg Thr Leu Thr Leu Phe Asn Val Thr 
225                 230                 235                 240 


Arg Asn Asp Ala Arg Ala Tyr Val Cys Gly Ile Gln Asn Ser Val Ser 
                245                 250                 255     


Ala Asn Arg Ser Asp Pro Val Thr Leu Asp Val Leu Tyr Gly Pro Asp 
            260                 265                 270         


Thr Pro Ile Ile Ser Pro Pro Asp Ser Ser Tyr Leu Ser Gly Ala Asp 
        275                 280                 285             


Leu Asn Leu Ser Cys His Ser Ala Ser Asn Pro Ser Pro Gln Tyr Ser 
    290                 295                 300                 


Trp Phe Val Asn Gly Thr Phe Gln Gln His Thr Gln Val Ile Phe Ile 
305                 310                 315                 320 


Thr Lys Ile Thr Pro Asn Asn Asn Gly Thr Tyr Ala Cys Phe Val Ser 
                325                 330                 335     


Asn Leu Ala Thr Gly Arg Asn Asn Ser Ile Val Lys Ser Ile Thr Val 
            340                 345                 350         


Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
        355                 360                 365             


Glu Ala Ala Ala Lys Ala Ser Arg 
    370                 375     


<210>  15
<211>  921
<212>  DNA
<213>  Artificial sequence

<220>
<223>  ClyA

<400>  15
atgactagta tttttgcaga acaaactgta gaggtagtta aaagcgcgat cgaaaccgca       60

gatggggcat tagatcttta taacaaatac ctcgaccagg tcatcccctg gaagaccttt      120

gatgaaacca taaaagagtt aagccgtttt aaacaggagt actcgcagga agcttctgtt      180

ttagttggtg atattaaagt tttgcttatg gacagccagg acaagtattt tgaagcgaca      240

caaactgttt atgaatggtg tggtgtcgtg acgcaattac tctcagcgta tattttacta      300

tttgatgaat ataatgagaa aaaagcatca gcccagaaag acattctcat taggatatta      360

gatgatggtg tcaagaaact gaatgaagcg caaaaatctc tcctgacaag ttcacaaagt      420

ttcaacaacg cttccggaaa actgctggca ttagatagcc agttaactaa tgatttttcg      480

gaaaaaagta gttatttcca gtcacaggtg gatagaattc gtaaggaagc ttatgccggt      540

gctgcagccg gcatagtcgc cggtccgttt ggattaatta tttcctattc tattgctgcg      600

ggcgtgattg aagggaaatt gattccagaa ttgaataaca ggctaaaaac agtgcaaaat      660

ttctttacta gcttatcagc tacagtgaaa caagcgaata aagatatcga tgcggcaaaa      720

ttgaaattag ccactgaaat agcagcaatt ggggagataa aaacggaaac cgaaacaacc      780

agattctacg ttgattatga tgatttaatg ctttctttat taaaaggagc tgcaaagaaa      840

atgattaaca cctgtaatga ataccaacaa cgtcatggta agaagacgct tttcgaggtt      900

cctgacgtcg ctagctgata a                                                921


<210>  16
<211>  305
<212>  PRT
<213>  Salmonella enterica

<400>  16

Met Thr Ser Ile Phe Ala Glu Gln Thr Val Glu Val Val Lys Ser Ala 
1               5                   10                  15      


Ile Glu Thr Ala Asp Gly Ala Leu Asp Leu Tyr Asn Lys Tyr Leu Asp 
            20                  25                  30          


Gln Val Ile Pro Trp Lys Thr Phe Asp Glu Thr Ile Lys Glu Leu Ser 
        35                  40                  45              


Arg Phe Lys Gln Glu Tyr Ser Gln Glu Ala Ser Val Leu Val Gly Asp 
    50                  55                  60                  


Ile Lys Val Leu Leu Met Asp Ser Gln Asp Lys Tyr Phe Glu Ala Thr 
65                  70                  75                  80  


Gln Thr Val Tyr Glu Trp Cys Gly Val Val Thr Gln Leu Leu Ser Ala 
                85                  90                  95      


Tyr Ile Leu Leu Phe Asp Glu Tyr Asn Glu Lys Lys Ala Ser Ala Gln 
            100                 105                 110         


Lys Asp Ile Leu Ile Arg Ile Leu Asp Asp Gly Val Lys Lys Leu Asn 
        115                 120                 125             


Glu Ala Gln Lys Ser Leu Leu Thr Ser Ser Gln Ser Phe Asn Asn Ala 
    130                 135                 140                 


Ser Gly Lys Leu Leu Ala Leu Asp Ser Gln Leu Thr Asn Asp Phe Ser 
145                 150                 155                 160 


Glu Lys Ser Ser Tyr Phe Gln Ser Gln Val Asp Arg Ile Arg Lys Glu 
                165                 170                 175     


Ala Tyr Ala Gly Ala Ala Ala Gly Ile Val Ala Gly Pro Phe Gly Leu 
            180                 185                 190         


Ile Ile Ser Tyr Ser Ile Ala Ala Gly Val Ile Glu Gly Lys Leu Ile 
        195                 200                 205             


Pro Glu Leu Asn Asn Arg Leu Lys Thr Val Gln Asn Phe Phe Thr Ser 
    210                 215                 220                 


Leu Ser Ala Thr Val Lys Gln Ala Asn Lys Asp Ile Asp Ala Ala Lys 
225                 230                 235                 240 


Leu Lys Leu Ala Thr Glu Ile Ala Ala Ile Gly Glu Ile Lys Thr Glu 
                245                 250                 255     


Thr Glu Thr Thr Arg Phe Tyr Val Asp Tyr Asp Asp Leu Met Leu Ser 
            260                 265                 270         


Leu Leu Lys Gly Ala Ala Lys Lys Met Ile Asn Thr Cys Asn Glu Tyr 
        275                 280                 285             


Gln Gln Arg His Gly Lys Lys Thr Leu Phe Glu Val Pro Asp Val Ala 
    290                 295                 300                 


Ser 
305 


<210>  17
<211>  985
<212>  DNA
<213>  Artificial sequence

<220>
<223>  LOA-linker- MUC1 ORF

<400>  17
ggatccaaaa taaggaggaa aaaaaatgaa agcgaccaaa ctggtgcttg gcgccgtgat       60

tctgggtagc acccttctgg ccggttgtag cagtaatgcg aaaatcgatc agggcattaa      120

cccgtatgtt ggctttgaaa tgggttacga ctggttaggt cgtatgccgt acaaaggcag      180

cgttgaaaac ggtgcataca aagctcaggg cgttcaactg accgctaaac tgggttaccc      240

gatcactgac gacctggaca tctacactcg tctgggtggc atggtatggc gtgcagacac      300

taaatccaac gtttatggta aaaaccacga caccggcgtt tctccggtct tcgctggcgg      360

tgttgagtac gcgatcactc ctgaaatcgc tacccgtctg gaataccagt ggaccaacaa      420

catcggtgac gcacacacca tcggcactcg tccggacaac ggcattccgg gtgcggaagc      480

cgctgcaaag gaggctgccg caaaagaagc agccgctaag gaggctgcgg caaaagcggc      540

tagaccggct cctggtagca cggcaccgcc tgcgcatggt gtaacttctg ctccggataa      600

tcgtcctgcg ttaggaagta ctgctcctcc ggctcacggg gtgacgagtg cacctgacac      660

gcgcccggca cctgggtcta cagcgccgcc tgcacatgga gttacatccg cgccggataa      720

tcgtcctgct ttgggatcga ctgcaccgcc tgcccatggg gtaaccagtg cccctgatac      780

ccgtccggca cctggctcaa cggcccctcc ggcacacggc gtcacgtcag caccggacaa      840

ccgtcctgct ttagggagca cagctccgcc tgcgcatgga gtcacttcag cgccggacac      900

acgccctgcc ccgggctcca ccgcccctcc ggcccacggt gtcacctcgg cccctgacac      960

ccgtcctagg taatgataga ctagt                                            985


<210>  18
<211>  315
<212>  PRT
<213>  Artificial sequence

<220>
<223>  LOA-linker- MUC1

<400>  18

Met Lys Ala Thr Lys Leu Val Leu Gly Ala Val Ile Leu Gly Ser Thr 
1               5                   10                  15      


Leu Leu Ala Gly Cys Ser Ser Asn Ala Lys Ile Asp Gln Gly Ile Asn 
            20                  25                  30          


Pro Tyr Val Gly Phe Glu Met Gly Tyr Asp Trp Leu Gly Arg Met Pro 
        35                  40                  45              


Tyr Lys Gly Ser Val Glu Asn Gly Ala Tyr Lys Ala Gln Gly Val Gln 
    50                  55                  60                  


Leu Thr Ala Lys Leu Gly Tyr Pro Ile Thr Asp Asp Leu Asp Ile Tyr 
65                  70                  75                  80  


Thr Arg Leu Gly Gly Met Val Trp Arg Ala Asp Thr Lys Ser Asn Val 
                85                  90                  95      


Tyr Gly Lys Asn His Asp Thr Gly Val Ser Pro Val Phe Ala Gly Gly 
            100                 105                 110         


Val Glu Tyr Ala Ile Thr Pro Glu Ile Ala Thr Arg Leu Glu Tyr Gln 
        115                 120                 125             


Trp Thr Asn Asn Ile Gly Asp Ala His Thr Ile Gly Thr Arg Pro Asp 
    130                 135                 140                 


Asn Gly Ile Pro Gly Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
145                 150                 155                 160 


Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Ala Ala Arg Pro Ala Pro 
                165                 170                 175     


Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Asn 
            180                 185                 190         


Arg Pro Ala Leu Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser 
        195                 200                 205             


Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His 
    210                 215                 220                 


Gly Val Thr Ser Ala Pro Asp Asn Arg Pro Ala Leu Gly Ser Thr Ala 
225                 230                 235                 240 


Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro 
                245                 250                 255     


Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Asn 
            260                 265                 270         


Arg Pro Ala Leu Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser 
        275                 280                 285             


Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His 
    290                 295                 300                 


Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Arg 
305                 310                 315 


<210>  19
<211>  2062
<212>  DNA
<213>  Artificial sequence

<220>
<223>  I198N-linker-A3B3-linker-MUC1

<400>  19
ggatccaaaa taaggaggaa aaaaaatgat ttttgcagaa caaactgtag aggtagttaa       60

aagcgcgatc gaaaccgcag atggggcatt agatctttat aacaaatacc tggaccaggt      120

catcccgtgg aagacctttg atgaaaccat taaagagtta agccgtttta aacaggagta      180

ctcgcaggaa gcttctgttt tagttggtga tattaaagtt ttgcttatgg acagccagga      240

caagtatttt gaagcgacac aaactgttta tgaatggtgt ggtgtcgtga cgcaattact      300

gtcagcgtat attttacttt ttgatgaata taatgagaaa aaagcatcag cccagaaaga      360

cattctgatc cgtattttag atgatggtgt caagaaactg aatgaagcgc aaaaatctct      420

tctgacaagt tcacaaagtt tcaacaacgc ttccggaaaa ctgcttgcat tagatagcca      480

gctgactaat gatttttcgg aaaaaagtag ttatttccag tcacaggtgg atcgcattcg      540

taaggaagct tatgccggtg ctgcagccgg catcgtcgcc ggtccgtttg gattaattat      600

ctcctattct aatgctgcgg gcgtgattga agggaaattg attccggaac tgaataaccg      660

tcttaaaaca gtgcaaaatt tctttactag cttatcagct acagtgaaac aagcgaataa      720

agatatcgat gcggcaaaat tgaaattagc cactgaaatc gcagcaatcg gggagatcaa      780

aacggaaacc gaaacaaccc gcttctacgt tgattatgat gatttaatgc tttctttgtt      840

aaaaggagct gcaaagaaaa tgattaacac ctgtaatgaa taccaacaac gtcatggtaa      900

gaagacgctt ttcgaggttc ctgacgtcgg cattccgggt gcggaagccg ctgcaaagga      960

ggctgccgca aaagaagcag ccgctaagga ggctgcggca aaagcggcta gcgtctctgc     1020

ggagctgccg aagccttcca tctccagcaa caactccaaa ccggtggagg acaaggatgc     1080

tgtggccttc acctgtgaac ctgaggctca gaacacaacc tacctttggt gggtaaatgg     1140

tcagagcctg ccggtcagtc ctcgtctgca gctttccaat ggcaaccgca ccctgactct     1200

tttcaatgtc acacgtaatg acgcacgcgc ctatgtatgt ggaatccaga actcagtgag     1260

tgcaaaccgc agtgaccctg tcaccctgga tgtcctttat gggccggaca ccccgatcat     1320

ttcccctccg gactcgtctt acctttcggg agcggattta aacctgtcct gtcactcggc     1380

ctctaaccct tccccgcagt attcttggtt tgtgaatggg acctttcagc aacacacaca     1440

agttattttt atcaccaaaa tcacgcctaa taataacggg acctatgcct gttttgtctc     1500

taacctggct actggccgca ataattccat tgtcaagagc atcacagtcg cggaagccgc     1560

tgcaaaggag gctgccgcaa aagaagcagc cgctaaggag gctgcggcaa aagcgtctag     1620

accggctcct ggtagcacgg caccgcctgc gcatggtgta acttctgctc cggataatcg     1680

tcctgcgtta ggaagtactg ctcctccggc tcacggggtg acgagtgcac ctgacacgcg     1740

cccggcacct gggtctacag cgccgcctgc acatggagtt acatccgcgc cggataatcg     1800

tcctgctttg ggatcgactg caccgcctgc ccatggggta accagtgccc ctgatacccg     1860

tccggcacct ggctcaacgg cccctccggc acacggcgtc acgtcagcac cggacaaccg     1920

tcctgcttta gggagcacag ctccgcctgc gcatggagtc acttcagcgc cggacacacg     1980

ccctgccccg ggctccaccg cccctccggc ccacggtgtc acctcggccc ctgacacccg     2040

tcctaggtaa tgatagacta gt                                              2062


<210>  20
<211>  674
<212>  PRT
<213>  Artificial sequence

<220>
<223>  I198N-linker-A3B3-linker-MUC1

<400>  20

Met Ile Phe Ala Glu Gln Thr Val Glu Val Val Lys Ser Ala Ile Glu 
1               5                   10                  15      


Thr Ala Asp Gly Ala Leu Asp Leu Tyr Asn Lys Tyr Leu Asp Gln Val 
            20                  25                  30          


Ile Pro Trp Lys Thr Phe Asp Glu Thr Ile Lys Glu Leu Ser Arg Phe 
        35                  40                  45              


Lys Gln Glu Tyr Ser Gln Glu Ala Ser Val Leu Val Gly Asp Ile Lys 
    50                  55                  60                  


Val Leu Leu Met Asp Ser Gln Asp Lys Tyr Phe Glu Ala Thr Gln Thr 
65                  70                  75                  80  


Val Tyr Glu Trp Cys Gly Val Val Thr Gln Leu Leu Ser Ala Tyr Ile 
                85                  90                  95      


Leu Leu Phe Asp Glu Tyr Asn Glu Lys Lys Ala Ser Ala Gln Lys Asp 
            100                 105                 110         


Ile Leu Ile Arg Ile Leu Asp Asp Gly Val Lys Lys Leu Asn Glu Ala 
        115                 120                 125             


Gln Lys Ser Leu Leu Thr Ser Ser Gln Ser Phe Asn Asn Ala Ser Gly 
    130                 135                 140                 


Lys Leu Leu Ala Leu Asp Ser Gln Leu Thr Asn Asp Phe Ser Glu Lys 
145                 150                 155                 160 


Ser Ser Tyr Phe Gln Ser Gln Val Asp Arg Ile Arg Lys Glu Ala Tyr 
                165                 170                 175     


Ala Gly Ala Ala Ala Gly Ile Val Ala Gly Pro Phe Gly Leu Ile Ile 
            180                 185                 190         


Ser Tyr Ser Asn Ala Ala Gly Val Ile Glu Gly Lys Leu Ile Pro Glu 
        195                 200                 205             


Leu Asn Asn Arg Leu Lys Thr Val Gln Asn Phe Phe Thr Ser Leu Ser 
    210                 215                 220                 


Ala Thr Val Lys Gln Ala Asn Lys Asp Ile Asp Ala Ala Lys Leu Lys 
225                 230                 235                 240 


Leu Ala Thr Glu Ile Ala Ala Ile Gly Glu Ile Lys Thr Glu Thr Glu 
                245                 250                 255     


Thr Thr Arg Phe Tyr Val Asp Tyr Asp Asp Leu Met Leu Ser Leu Leu 
            260                 265                 270         


Lys Gly Ala Ala Lys Lys Met Ile Asn Thr Cys Asn Glu Tyr Gln Gln 
        275                 280                 285             


Arg His Gly Lys Lys Thr Leu Phe Glu Val Pro Asp Val Gly Ile Pro 
    290                 295                 300                 


Gly Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala 
305                 310                 315                 320 


Lys Glu Ala Ala Ala Lys Ala Ala Ser Val Ser Ala Glu Leu Pro Lys 
                325                 330                 335     


Pro Ser Ile Ser Ser Asn Asn Ser Lys Pro Val Glu Asp Lys Asp Ala 
            340                 345                 350         


Val Ala Phe Thr Cys Glu Pro Glu Ala Gln Asn Thr Thr Tyr Leu Trp 
        355                 360                 365             


Trp Val Asn Gly Gln Ser Leu Pro Val Ser Pro Arg Leu Gln Leu Ser 
    370                 375                 380                 


Asn Gly Asn Arg Thr Leu Thr Leu Phe Asn Val Thr Arg Asn Asp Ala 
385                 390                 395                 400 


Arg Ala Tyr Val Cys Gly Ile Gln Asn Ser Val Ser Ala Asn Arg Ser 
                405                 410                 415     


Asp Pro Val Thr Leu Asp Val Leu Tyr Gly Pro Asp Thr Pro Ile Ile 
            420                 425                 430         


Ser Pro Pro Asp Ser Ser Tyr Leu Ser Gly Ala Asp Leu Asn Leu Ser 
        435                 440                 445             


Cys His Ser Ala Ser Asn Pro Ser Pro Gln Tyr Ser Trp Phe Val Asn 
    450                 455                 460                 


Gly Thr Phe Gln Gln His Thr Gln Val Ile Phe Ile Thr Lys Ile Thr 
465                 470                 475                 480 


Pro Asn Asn Asn Gly Thr Tyr Ala Cys Phe Val Ser Asn Leu Ala Thr 
                485                 490                 495     


Gly Arg Asn Asn Ser Ile Val Lys Ser Ile Thr Val Ala Glu Ala Ala 
            500                 505                 510         


Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala 
        515                 520                 525             


Lys Ala Ser Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His Gly 
    530                 535                 540                 


Val Thr Ser Ala Pro Asp Asn Arg Pro Ala Leu Gly Ser Thr Ala Pro 
545                 550                 555                 560 


Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro Gly 
                565                 570                 575     


Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Asn Arg 
            580                 585                 590         


Pro Ala Leu Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala 
        595                 600                 605             


Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His Gly 
    610                 615                 620                 


Val Thr Ser Ala Pro Asp Asn Arg Pro Ala Leu Gly Ser Thr Ala Pro 
625                 630                 635                 640 


Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro Gly 
                645                 650                 655     


Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr Arg 
            660                 665                 670         


Pro Arg 
        


<210>  21
<211>  145
<212>  PRT
<213>  Artificial sequence

<220>
<223>  lpp-ompA

<400>  21

Met Lys Ala Thr Lys Leu Val Leu Gly Ala Val Ile Leu Gly Ser Thr 
1               5                   10                  15      


Leu Leu Ala Gly Cys Ser Ser Asn Ala Lys Ile Asp Gln Gly Ile Asn 
            20                  25                  30          


Pro Tyr Val Gly Phe Glu Met Gly Tyr Asp Trp Leu Gly Arg Met Pro 
        35                  40                  45              


Tyr Lys Gly Ser Val Glu Asn Gly Ala Tyr Lys Ala Gln Gly Val Gln 
    50                  55                  60                  


Leu Thr Ala Lys Leu Gly Tyr Pro Ile Thr Asp Asp Leu Asp Ile Tyr 
65                  70                  75                  80  


Thr Arg Leu Gly Gly Met Val Trp Arg Ala Asp Thr Lys Ser Asn Val 
                85                  90                  95      


Tyr Gly Lys Asn His Asp Thr Gly Val Ser Pro Val Phe Ala Gly Gly 
            100                 105                 110         


Val Glu Tyr Ala Ile Thr Pro Glu Ile Ala Thr Arg Leu Glu Tyr Gln 
        115                 120                 125             


Trp Thr Asn Asn Ile Gly Asp Ala His Thr Ile Gly Thr Arg Pro Asp 
    130                 135                 140                 


Asn 
145 


<210>  22
<211>  21
<212>  PRT
<213>  Artificial sequence

<220>
<223>  linker sequence

<400>  22

Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
1               5                   10                  15      


Glu Ala Ala Ala Lys 
            20      


<210>  23
<211>  179
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CEA A3B3

<400>  23

Val Ser Ala Glu Leu Pro Lys Pro Ser Ile Ser Ser Asn Asn Ser Lys 
1               5                   10                  15      


Pro Val Glu Asp Lys Asp Ala Val Ala Phe Thr Cys Glu Pro Glu Ala 
            20                  25                  30          


Gln Asn Thr Thr Tyr Leu Trp Trp Val Asn Gly Gln Ser Leu Pro Val 
        35                  40                  45              


Ser Pro Arg Leu Gln Leu Ser Asn Gly Asn Arg Thr Leu Thr Leu Phe 
    50                  55                  60                  


Asn Val Thr Arg Asn Asp Ala Arg Ala Tyr Val Cys Gly Ile Gln Asn 
65                  70                  75                  80  


Ser Val Ser Ala Asn Arg Ser Asp Pro Val Thr Leu Asp Val Leu Tyr 
                85                  90                  95      


Gly Pro Asp Thr Pro Ile Ile Ser Pro Pro Asp Ser Ser Tyr Leu Ser 
            100                 105                 110         


Gly Ala Asp Leu Asn Leu Ser Cys His Ser Ala Ser Asn Pro Ser Pro 
        115                 120                 125             


Gln Tyr Ser Trp Phe Val Asn Gly Thr Phe Gln Gln His Thr Gln Val 
    130                 135                 140                 


Ile Phe Ile Thr Lys Ile Thr Pro Asn Asn Asn Gly Thr Tyr Ala Cys 
145                 150                 155                 160 


Phe Val Ser Asn Leu Ala Thr Gly Arg Asn Asn Ser Ile Val Lys Ser 
                165                 170                 175     


Ile Thr Val 
            


<210>  24
<211>  142
<212>  PRT
<213>  Artificial sequence

<220>
<223>  MUC-1

<400>  24

Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala 
1               5                   10                  15      


Pro Asp Asn Arg Pro Ala Leu Gly Ser Thr Ala Pro Pro Ala His Gly 
            20                  25                  30          


Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala Pro 
        35                  40                  45              


Pro Ala His Gly Val Thr Ser Ala Pro Asp Asn Arg Pro Ala Leu Gly 
    50                  55                  60                  


Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr Arg 
65                  70                  75                  80  


Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala 
                85                  90                  95      


Pro Asp Asn Arg Pro Ala Leu Gly Ser Thr Ala Pro Pro Ala His Gly 
            100                 105                 110         


Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala Pro 
        115                 120                 125             


Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Arg 
    130                 135                 140         


<210>  25
<211>  301
<212>  PRT
<213>  Artificial sequence

<220>
<223>  ClyAI198N

<400>  25

Met Ile Phe Ala Glu Gln Thr Val Glu Val Val Lys Ser Ala Ile Glu 
1               5                   10                  15      


Thr Ala Asp Gly Ala Leu Asp Leu Tyr Asn Lys Tyr Leu Asp Gln Val 
            20                  25                  30          


Ile Pro Trp Lys Thr Phe Asp Glu Thr Ile Lys Glu Leu Ser Arg Phe 
        35                  40                  45              


Lys Gln Glu Tyr Ser Gln Glu Ala Ser Val Leu Val Gly Asp Ile Lys 
    50                  55                  60                  


Val Leu Leu Met Asp Ser Gln Asp Lys Tyr Phe Glu Ala Thr Gln Thr 
65                  70                  75                  80  


Val Tyr Glu Trp Cys Gly Val Val Thr Gln Leu Leu Ser Ala Tyr Ile 
                85                  90                  95      


Leu Leu Phe Asp Glu Tyr Asn Glu Lys Lys Ala Ser Ala Gln Lys Asp 
            100                 105                 110         


Ile Leu Ile Arg Ile Leu Asp Asp Gly Val Lys Lys Leu Asn Glu Ala 
        115                 120                 125             


Gln Lys Ser Leu Leu Thr Ser Ser Gln Ser Phe Asn Asn Ala Ser Gly 
    130                 135                 140                 


Lys Leu Leu Ala Leu Asp Ser Gln Leu Thr Asn Asp Phe Ser Glu Lys 
145                 150                 155                 160 


Ser Ser Tyr Phe Gln Ser Gln Val Asp Arg Ile Arg Lys Glu Ala Tyr 
                165                 170                 175     


Ala Gly Ala Ala Ala Gly Ile Val Ala Gly Pro Phe Gly Leu Ile Ile 
            180                 185                 190         


Ser Tyr Ser Asn Ala Ala Gly Val Ile Glu Gly Lys Leu Ile Pro Glu 
        195                 200                 205             


Leu Asn Asn Arg Leu Lys Thr Val Gln Asn Phe Phe Thr Ser Leu Ser 
    210                 215                 220                 


Ala Thr Val Lys Gln Ala Asn Lys Asp Ile Asp Ala Ala Lys Leu Lys 
225                 230                 235                 240 


Leu Ala Thr Glu Ile Ala Ala Ile Gly Glu Ile Lys Thr Glu Thr Glu 
                245                 250                 255     


Thr Thr Arg Phe Tyr Val Asp Tyr Asp Asp Leu Met Leu Ser Leu Leu 
            260                 265                 270         


Lys Gly Ala Ala Lys Lys Met Ile Asn Thr Cys Asn Glu Tyr Gln Gln 
        275                 280                 285             


Arg His Gly Lys Lys Thr Leu Phe Glu Val Pro Asp Val 
    290                 295                 300     


<210>  26
<211>  745
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Francisella novicida (lpxEFn ) synthetic gene sequence

<400>  26
aaaataagga ggaaaaaaaa tgctgaaaca gaccctgcaa acgaactttc agggttttaa       60

agatattttt aagaaaccga aactacacaa tcataaattg cctcgctatc tacagttgaa      120

atatacgttt ataccattat taattttggt aatttttgca tactataact tagatacccc      180

agttgagaac tatatcaagc attctatgcc aaatattgtt ggtgtaattt ttggtaaaat      240

aactgatgtt ggtaaggccg agtatatttt gataatttgc ggtgtgatag tgttagcgcg      300

tttatttaca gatagccaaa aattatctgc taatactcgc gctatgtttg acaaggtgtc      360

ggcatatgcg ggttttatct tagcaactgt agctattagt ggtattttgg gacaaatact      420

caagatgata ataggtcgcg cgcgtcctaa gtttttcttg gaatatggtt cgcattattt      480

ccaacatttt catgcacctg gatatgattt tgcaagtatg ccgtcagggc actcaatcac      540

agttggagca atgtttatag cattttttta tattttccct aagctacgct atttttggta      600

tttgctgata gtggtatttg ctgggagtcg tattatggtt ggttcacatt atcctagtga      660

tgtaattttt ggcgttgctt ttggttgtta ctgtacagca tatatctact attggatgcg      720

caatcgtgag attatttagt gataa                                            745


<210>  27
<211>  239
<212>  PRT
<213>  Francisella novicida

<400>  27

Met Leu Lys Gln Thr Leu Gln Thr Asn Phe Gln Gly Phe Lys Asp Ile 
1               5                   10                  15      


Phe Lys Lys Pro Lys Leu His Asn His Lys Leu Pro Arg Tyr Leu Gln 
            20                  25                  30          


Leu Lys Tyr Thr Phe Ile Pro Leu Leu Ile Leu Val Ile Phe Ala Tyr 
        35                  40                  45              


Tyr Asn Leu Asp Thr Pro Val Glu Asn Tyr Ile Lys His Ser Met Pro 
    50                  55                  60                  


Asn Ile Val Gly Val Ile Phe Gly Lys Ile Thr Asp Val Gly Lys Ala 
65                  70                  75                  80  


Glu Tyr Ile Leu Ile Ile Cys Gly Val Ile Val Leu Ala Arg Leu Phe 
                85                  90                  95      


Thr Asp Ser Gln Lys Leu Ser Ala Asn Thr Arg Ala Met Phe Asp Lys 
            100                 105                 110         


Val Ser Ala Tyr Ala Gly Phe Ile Leu Ala Thr Val Ala Ile Ser Gly 
        115                 120                 125             


Ile Leu Gly Gln Ile Leu Lys Met Ile Ile Gly Arg Ala Arg Pro Lys 
    130                 135                 140                 


Phe Phe Leu Glu Tyr Gly Ser His Tyr Phe Gln His Phe His Ala Pro 
145                 150                 155                 160 


Gly Tyr Asp Phe Ala Ser Met Pro Ser Gly His Ser Ile Thr Val Gly 
                165                 170                 175     


Ala Met Phe Ile Ala Phe Phe Tyr Ile Phe Pro Lys Leu Arg Tyr Phe 
            180                 185                 190         


Trp Tyr Leu Leu Ile Val Val Phe Ala Gly Ser Arg Ile Met Val Gly 
        195                 200                 205             


Ser His Tyr Pro Ser Asp Val Ile Phe Gly Val Ala Phe Gly Cys Tyr 
    210                 215                 220                 


Cys Thr Ala Tyr Ile Tyr Tyr Trp Met Arg Asn Arg Glu Ile Ile 
225                 230                 235                 


<210>  28
<211>  435
<212>  DNA
<213>  Artificial sequence

<220>
<223>  lpp-ompA sequence

<400>  28
atgaaagcga ccaaactggt gcttggcgcc gtgattctgg gtagcaccct tctggccggt       60

tgtagcagta atgcgaaaat cgatcagggc attaacccgt atgttggctt tgaaatgggt      120

tacgactggt taggtcgtat gccgtacaaa ggcagcgttg aaaacggtgc atacaaagct      180

cagggcgttc aactgaccgc taaactgggt tacccgatca ctgacgacct ggacatctac      240

actcgtctgg gtggcatggt atggcgtgca gacactaaat ccaacgttta tggtaaaaac      300

cacgacaccg gcgtttctcc ggtcttcgct ggcggtgttg agtacgcgat cactcctgaa      360

atcgctaccc gtctggaata ccagtggacc aacaacatcg gtgacgcaca caccatcggc      420

actcgtccgg acaac                                                       435


<210>  29
<211>  1521
<212>  DNA
<213>  Salmonella enterica

<400>  29
atggcacaag tcattaatac aaacagcctg tcgctgttga cccagaataa cctgaacaaa       60

tcccagtccg cactgggcac tgctatcgag cgtttgtctt ccggtctgcg tatcaacagc      120

gcgaaagacg atgcggcagg acaggcgatt gctaaccgtt ttaccgcgaa catcaaaggt      180

ctgactcagg cttcccgtaa cgctaacgac ggtatctcca ttgcgcagac cactgaaggc      240

gcgctgaacg aaatcaacaa caacctgcag cgtgtgcgtg aactggcggt tcagtctgcg      300

aatggtacta actcccagtc tgacctcgac tccatccagg ctgaaatcac ccagcgcctg      360

aacgaaatcg accgtgtatc cggccagact cagttcaacg gcgtgaaagt cctggcgcag      420

gacaacaccc tgaccatcca ggttggtgcc aacgacggtg aaactatcga tattgattta      480

aaagaaatca gctctaaaac actgggactt gataagctta atgtccaaga tgcctacacc      540

ccgaaagaaa ctgctgtaac cgttgataaa actacctata aaaatggtac agatcctatt      600

acagcccaga gcaatactga tatccaaact gcaattggcg gtggtgcaac gggggttact      660

ggggctgata tcaaatttaa agatggtcaa tactatttag atgttaaagg cggtgcttct      720

gctggtgttt ataaagccac ttatgatgaa actacaaaga aagttaatat tgatacgact      780

gataaaactc cgttggcaac tgcggaagct acagctattc ggggaacggc cactataacc      840

cacaaccaaa ttgctgaagt aacaaaagag ggtgttgata cgaccacagt tgcggctcaa      900

cttgctgcag caggggttac tggcgccgat aaggacaata ctagccttgt aaaactatcg      960

tttgaggata aaaacggtaa ggttattgat ggtggctatg cagtgaaaat gggcgacgat     1020

ttctatgccg ctacatatga tgagaaaaca ggtgcaatta ctgctaaaac cactacttat     1080

acagatggta ctggcgttgc tcaaactgga gctgtgaaat ttggtggcgc aaatggtaaa     1140

tctgaagttg ttactgctac cgatggtaag acttacttag caagcgacct tgacaaacat     1200

aacttcagaa caggcggtga gcttaaagag gttaatacag ataagactga aaacccactg     1260

cagaaaattg atgctgcctt ggcacaggtt gatacacttc gttctgacct gggtgcggtt     1320

cagaaccgtt tcaactccgc tatcaccaac ctgggcaata ccgtaaataa cctgtcttct     1380

gcccgtagcc gtatcgaaga ttccgactac gcaaccgaag tctccaacat gtctcgcgcg     1440

cagattctgc agcaggccgg tacctccgtt ctggcgcagg cgaaccaggt tccgcaaaac     1500

gtcctctctt tactgcgtta a                                               1521


<210>  30
<211>  537
<212>  DNA
<213>  Artificial sequence

<220>
<223>   A3B3

<400>  30
gtctctgcgg agctgccgaa gccttccatc tccagcaaca actccaaacc ggtggaggac       60

aaggatgctg tggccttcac ctgtgaacct gaggctcaga acacaaccta cctttggtgg      120

gtaaatggtc agagcctgcc ggtcagtcct cgtctgcagc tttccaatgg caaccgcacc      180

ctgactcttt tcaatgtcac acgtaatgac gcacgcgcct atgtatgtgg aatccagaac      240

tcagtgagtg caaaccgcag tgaccctgtc accctggatg tcctttatgg gccggacacc      300

ccgatcattt cccctccgga ctcgtcttac ctttcgggag cggatttaaa cctgtcctgt      360

cactcggcct ctaacccttc cccgcagtat tcttggtttg tgaatgggac ctttcagcaa      420

cacacacaag ttatttttat caccaaaatc acgcctaata ataacgggac ctatgcctgt      480

tttgtctcta acctggctac tggccgcaat aattccattg tcaagagcat cacagtc         537


<210>  31
<211>  426
<212>  DNA
<213>  Artificial sequence

<220>
<223>  MUC-1

<400>  31
ccggctcctg gtagcacggc accgcctgcg catggtgtaa cttctgctcc ggataatcgt       60

cctgcgttag gaagtactgc tcctccggct cacggggtga cgagtgcacc tgacacgcgc      120

ccggcacctg ggtctacagc gccgcctgca catggagtta catccgcgcc ggataatcgt      180

cctgctttgg gatcgactgc accgcctgcc catggggtaa ccagtgcccc tgatacccgt      240

ccggcacctg gctcaacggc ccctccggca cacggcgtca cgtcagcacc ggacaaccgt      300

cctgctttag ggagcacagc tccgcctgcg catggagtca cttcagcgcc ggacacacgc      360

cctgccccgg gctccaccgc ccctccggcc cacggtgtca cctcggcccc tgacacccgt      420

cctagg                                                                 426


