                         SEQUENCE LISTING

<110>  BASF SE

<120>  Shuttle vector for expression in E. coli and Bacilli

<130> 191608WO01

<160>  104

<170>  According Wipo Std 25



<210> 1
<211> 32
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of pE194

<400> 1
tatatacagc tggattcaca aaaaataggc ac                                   32

<210> 2
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of pE194

<400> 2
tatatacagc tggattatgt cttttgcgca gtc                                  33

<210> 3
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of functional mRFP 
      cassette

<400> 3
tatatggatc cgtaatcagg gtatcgaggc                                      30

<210> 4
<211> 31
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of functional mRFP
      cassette

<400> 4
tatatggatc cctcattagg cgggctacta a                                    31

<210> 5
<211> 1345
<212> DNA
<213> Artificial Sequence

<220>
<223> functional fragment: T2A mRFP cassette

<400> 5
ggaatagatc tggccaacga ggcctcgagg atccgatatc atgcatggcg cgccaccctg     60
agacgaccct gatgcaggtg accctgagac cttcgcgccc agctgtctag ggcggcggat    120
ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt    180
tcgactgagc ctttcgtttt atttgatgcc tttaattaag taccttgtcg gataaagctg    240
tgttatatta tgtcttggtg ttaaatacac acgcttaacg atttatgcag agggtgctgc    300
aggcggcagt tctgtacaaa aatgacctaa gcggaggaaa aaaaccatta tattaggagg    360
aaataacatg gcctcttcag aggatgttat taaagaattt atgcggttta aggtgaggat    420
ggaaggctcg gtgaacggac atgagttcga aattgaggga gaaggtgaag gccgccctta    480
tgaaggtact cagacagcga aattgaaagt cacgaaaggc ggaccgctgc cgtttgcttg    540
ggacattctc tcacctcaat ttcaatatgg ctcaaaagcc tacgtaaaac acccggctga    600
catccctgat tacttaaagc tatccttccc ggagggcttt aaatgggaac gagttatgaa    660
ttttgaggac ggcggcgtcg ttactgtcac acaggattct tcccttcagg atggcgaatt    720
tatttacaaa gtaaaacttc gtggaactaa cttcccaagt gatggtcccg tgatgcaaaa    780
aaaaacaatg ggatgggaag catctacgga acgtatgtat ccggaggatg gagccttaaa    840
gggtgaaatc aaaatgcgcc tgaaacttaa agatggcgga cactatgacg cggaagttaa    900
aacaacatat atggctaaaa aaccagtcca actgccggga gcatataaga cggatataaa    960
gttggacatt accagccata atgaagatta cacgattgtg gaacagtatg agagagcaga   1020
gggcagacat agcacaggcg cgtaagaatt aatgaaaaat aagcggcagc ctgcttttcc   1080
atgcgggctg ccgcttatcg ggttattgtc gtgactggga aaaccctggc gactagtctt   1140
ggactcctgt tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg   1200
ctcggttgcc gccgggcgtt ttttattggt gagaatccag gggtccccaa taattacgat   1260
ttggtctcac tcacacctgc tcgtctcact caatttaaat ggcggccgcg gatcctcgac   1320
gggccaataa ggccagatct ggatt                                         1345

<210> 6
<211> 1012
<212> DNA
<213> Artificial Sequence

<220>
<223> homology regions: Fusion of 5- and 3-prime regions of amyB
      gene with flanking XmaI sites

<400> 6
cccgggataa tgccgtcgca ctggccgata ttgagagatt tccttgtgac aagctgcaaa     60
gcataatgat gacggtccag ctcgcggctg attcccgtta acagattcat ataataaggt    120
tctgttgtat ccatttcttc cagtatgagc agcttgacga cctgtgttct gttttgaacg    180
agcgctcttg ctgcatagtt cggtatataa ttgagctcct tcattgcgga atgaacaagc    240
tttttcaatt catccgtcac agtctcagga tgattgatca cccgcgatac cgtcattttc    300
gacacatttg ctttctttgc tacatcagat aacgttgcca tttcatcccc gccttaccta    360
tgcgattcaa actgtcagca agtccttcct gagggctgat gacactttgt taaaattaat    420
tataaaatgt aatcaaagaa atttataaga cgggcaaaat aaaaaaacgg atttccttca    480
ggaaatccgt cctctctgct cttctagatt ctcctcccct ttcaatgtga aacatatgat    540
attgtataaa tattccgaat ttttaacaaa taccattttc cctatatttt cttccaaaag    600
aaaagcgccg atatggcgct ttctactcat ttattcaata gcctctctgc ttcttcactt    660
cttcaagctg agatacagtt accaattgat agccttttgc tttcagcttt ttaataatct    720
cttcagcagc atctgcggac gttgcataaa tatcgtgcat taagacgatt tttccgtctc    780
ccgcatggct catgacatga ttgacaatct tttgcttatt tttgtacttc caatcttccg    840
gatcaacatc ccacaatgaa accttcagat tggaaagcga gcggacggaa tcattgatcc    900
cgccgtatgg aggacgcaag tgtacaggca ggtgtccgct gattttttcg atcatttctt    960
gcgtgtcgtt aatctcctga tacgcttttt cgtttgacag ccttgtcccg gg           1012

<210> 7
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: Protospacer target sequence for amyB gene
      with 4nt 5-prime extension

<400> 7
tacgtcgcag cagaaattaa gaga                                            24

<210> 8
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: Protospacer target sequence for amyB gene
      with 4nt 5-prime extension

<400> 8
aaactctctt aatttctgct gcga                                            24

<210> 9
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of amyB genomic region

<400> 9
ttgcccgaat acaacgacag                                                 20

<210> 10
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of amyB genomic region

<400> 10
caacaacagg ctgtctgacg g                                               21

<210> 11
<211> 2143
<212> DNA
<213> Bacillus licheniforms

<220>
<223> P300 DSM641 RMS: RMS region and flanking region

<400> 11
aacttctata aatgtaacga tacgatttat tgtttcatta aagtcttcct ttatttcatg     60
ttcccatatt cttttaatgt tccaatcctt ttcctcgtaa tatttattaa cttccttatc    120
tcttttttta tttctttcga gttttttctc ccaatattcc gtattacttt ttggtatatt    180
cccgtgtttt tcacacgcat gccagaaaca agaatcaatg aatatgacta ttttatattt    240
ctgtattact atatctggac taccgtataa tttcttaaca ttttttcgga atcttattcc    300
acggtgccat agttctttag taaccttatc ttctaatttt gaacgagatt tgattgcctg    360
catgtttttt cttctttgtt cttttgaaac cgtgtcagtc atagaagagt cctccaaagc    420
cacaataatt gtattctata aacgaggaag caagccctca agcttacccc ctcttagttc    480
cttttttgcc tacttattta tttgttttca ttttcaaatt catcataaga acccatttca    540
caaaaatcaa tggagtaatg ttgttcgctg tgtttataaa caattactga gtcaatattt    600
agtctttcca gcatttcata ggttagaagt tctttttcct ctaagttcat aacttgtctt    660
agtagccatt ctccaagagc actatttgga ttagacataa gtgctttact attgtcttgg    720
cataccttgg ctgataaaag cgatttgtct ggcaagcgta actgaaaagg tttatcacga    780
gctgggaaaa atgttgggaa tacattatga atccattttg gaattggtat ataaatctcg    840
ttagggtttc gtggtcgacc taaagcattc cattggttta gaccgctttt ttctggtaca    900
tgacgctttg agccacggtc tgaaaagagt ggaagaataa cgtgctcaag gttttcaaaa    960
ggattgacag ttggtgctgg aatttttgga atttcaaagc caaatagttt agccaattca   1020
tgataaggat tttctaagat ttcaacatta atttcttcaa taggtttatc agtgataaaa   1080
cgcttataaa gggtgctctt agtgacatta aagctgtatt cgtgtagacc gtcttcaaag   1140
gtgattgtat ttctgttgtt acttactttc acatttgtaa ttgaggagat ttcaaccaag   1200
tccattggct cttcaaaaat aagaattttc cctggctttc ttgttacaca gtggtatatc   1260
attgaatcaa taccatatgt tcttttagta aattcaattc tctcgttacg gagagaagca   1320
accgtgttta ttagctcttt tggagatttc ccacgataca agtctgagtc tttattgaat   1380
tcagctactt tttgaagagt atgaccatta ccatgaagaa aagtcttaat accaattccg   1440
acacgattta atgaagcgtc agcagaacag tctgacctcc ccaagttttc agctccaaat   1500
gcttcacaaa aagcattttc cacattcctt gagaccaaat aaggcgagtc actttcagag   1560
aacaaattgg atagcgaacc agttgagcgg agcatttgtt tgtatgtagt gcagttgatg   1620
gctggttgat tagtatagaa cattattttt cctcctcttt tatgcttgtc atttcttctt   1680
tcagacccaa aaggtagtca gctgatacgt tcaatgtttc agctattctt ttgaaagtgt   1740
ccaatgatgg agttctattt tcactttcat atagtgacca agtgcttcta gtgaccccga   1800
ctttttcagc gatttggctg ggtaataacc tacgagcttc tcttgcattt tgaatacgat   1860
ttccaaggaa aggtatcatt tttgcacctc caagatttgt tgttttcaga gtatcaccag   1920
aacccccgaa aatagtccaa agttagctaa cagcaaacaa ataaaaataa ataagttgtt   1980
tactcttagc aaacttgtta ctaaaatttg ataaagttat tcatttaatc cagctcttat   2040
gctaaaattg cattagcgga caagcttaat gtttgcaagg aggtataatt ttgacttatc   2100
gagtaggtag tatgtttgct gggataggtg gaacttgttt agg                     2143

<210> 12
<211> 1146
<212> DNA
<213> Bacillus licheniforms

<220>
<223> cds: Restrictase P300 DSM641

<400> 12
ttatttgttt tcattttcaa attcatcata agaacccatt tcacaaaaat caatggagta     60
atgttgttcg ctgtgtttat aaacaattac tgagtcaata tttagtcttt ccagcatttc    120
ataggttaga agttcttttt cctctaagtt cataacttgt cttagtagcc attctccaag    180
agcactattt ggattagaca taagtgcttt actattgtct tggcatacct tggctgataa    240
aagcgatttg tctggcaagc gtaactgaaa aggtttatca cgagctggga aaaatgttgg    300
gaatacatta tgaatccatt ttggaattgg tatataaatc tcgttagggt ttcgtggtcg    360
acctaaagca ttccattggt ttagaccgct tttttctggt acatgacgct ttgagccacg    420
gtctgaaaag agtggaagaa taacgtgctc aaggttttca aaaggattga cagttggtgc    480
tggaattttt ggaatttcaa agccaaatag tttagccaat tcatgataag gattttctaa    540
gatttcaaca ttaatttctt caataggttt atcagtgata aaacgcttat aaagggtgct    600
cttagtgaca ttaaagctgt attcgtgtag accgtcttca aaggtgattg tatttctgtt    660
gttacttact ttcacatttg taattgagga gatttcaacc aagtccattg gctcttcaaa    720
aataagaatt ttccctggct ttcttgttac acagtggtat atcattgaat caataccata    780
tgttctttta gtaaattcaa ttctctcgtt acggagagaa gcaaccgtgt ttattagctc    840
ttttggagat ttcccacgat acaagtctga gtctttattg aattcagcta ctttttgaag    900
agtatgacca ttaccatgaa gaaaagtctt aataccaatt ccgacacgat ttaatgaagc    960
gtcagcagaa cagtctgacc tccccaagtt ttcagctcca aatgcttcac aaaaagcatt   1020
ttccacattc cttgagacca aataaggcga gtcactttca gagaacaaat tggatagcga   1080
accagttgag cggagcattt gtttgtatgt agtgcagttg atggctggtt gattagtata   1140
gaacat                                                              1146

<210> 13
<211> 1022
<212> DNA
<213> Artificial Sequence

<220>
<223> homology regions: Fusion of 5- and 3-prime regions of
      restrictase gene with flanking BsaI sites

<400> 13
ggtctcgacc caacttctat aaatgtaacg atacgattta ttgtttcatt aaagtcttcc     60
tttatttcat gttcccatat tcttttaatg ttccaatcct tttcctcgta atatttatta    120
acttccttat ctcttttttt atttctttcg agttttttct cccaatattc cgtattactt    180
tttggtatat tcccgtgttt ttcacacgca tgccagaaac aagaatcaat gaatatgact    240
attttatatt tctgtattac tatatctgga ctaccgtata atttcttaac attttttcgg    300
aatcttattc cacggtgcca tagttcttta gtaaccttat cttctaattt tgaacgagat    360
ttgattgcct gcatgttttt tcttctttgt tcttttgaaa ccgtgtcagt catagaagag    420
tcctccaaag ccacaataat tgtattctat aaacgaggaa gcaagccctc aagcttaccc    480
cctcttagtt ccttttttgc ctacttattt atatttttcc tcctctttta tgcttgtcat    540
ttcttctttc agacccaaaa ggtagtcagc tgatacgttc aatgtttcag ctattctttt    600
gaaagtgtcc aatgatggag ttctattttc actttcatat agtgaccaag tgcttctagt    660
gaccccgact ttttcagcga tttggctggg taataaccta cgagcttctc ttgcattttg    720
aatacgattt ccaaggaaag gtatcatttt tgcacctcca agatttgttg ttttcagagt    780
atcaccagaa cccccgaaaa tagtccaaag ttagctaaca gcaaacaaat aaaaataaat    840
aagttgttta ctcttagcaa acttgttact aaaatttgat aaagttattc atttaatcca    900
gctcttatgc taaaattgca ttagcggaca agcttaatgt ttgcaaggag gtataatttt    960
gacttatcga gtaggtagta tgtttgctgg gataggtgga acttgtttag gctcaggaga   1020
cc                                                                  1022

<210> 14
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of restrictase genomic
      region

<400> 14
gacaatcccc ttttactgac c                                               21

<210> 15
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of restrictase genomic
      region

<400> 15
ctatttcatt tgcccagaca atcc                                            24

<210> 16
<211> 1363
<212> DNA
<213> Artificial Sequence

<220>
<223> homology regions: Fusion of 5- and 3-prime regions of pga
      genes with flanking BsaI sites

<400> 16
ggtctcgacc cgaacactga aattttagac cgggctggga tatcgagaca tatatagggg     60
cgtttaatgg cgaatacagt aacaatgaga atagtaagaa aaattaaaaa tgttaaagtt    120
tgatgaatta tcattgaaaa aaattaatgg ctttttaaat cctaggattt taacctaaaa    180
tctgaagaaa taaggtggat cgaacgactc acaaaatatt tggatttgtc aatgaatccc    240
gctttatgct aaaagagatt ttcatttttt gatagatggt ctgattgtca taggacggat    300
ttgttttgaa gagggaacat tggtgacttt ttaacctgtt cgaaaagagc gaaaatacta    360
aaagaaaaga gacatcccgg ctgacagccc atttaaaggg gattgcggcc gggggaaaaa    420
agagatcctg aatccatcct tcaacctttc atctgaaata gggagaaaag tacaaaaatc    480
ataatgtcga attttgaaag cgcatactta aaacgctgac aaaaatctga taggaattaa    540
gaactttcga tttccaaaaa tatcaataaa aagataggca ttaatgactc gggcgaggtg    600
atctttgtca cggaaaattt cgtcgtcttc tgttacataa tgccgattgt gatttcatag    660
tgaaccctga tcccggttat aaaagacctg tgaaaagcgg ccggtttgaa agggaaacac    720
gacaattttc ttaaccggtc agtgtataaa gttttataga aaatcaggag gatatataca    780
tggttttggg gttcatgttt attgtattct tttgaaggga ataaaaactg acaaatttcg    840
actgaagcaa aatttgaaaa tgcatcacct taccaattcg ggatgggaac cgcacctcat    900
gttcatgacc tctttagaat atttcccttc atctttttaa tccgcgctta ggtgaaaaag    960
ctgatcatgc tgtgctgagc gtttcttctc gctatgacgc tgctgtacat gcaaaaaaag   1020
tcctttaaat atcccagttg aatgacgatg aaagaggaaa gaagaggagg aacagatcaa   1080
ttgataaaaa aagcggcaaa caaaaagttg gttttgtttt gtggaattgc ggtgctttgg   1140
atgtctttat ttttaacgaa tcataatgat gtacgcgccg atacgatcgg cgagaaaata   1200
gcggaaactg ccagacagct tgagggtgcg aaatacagct acggcggaga gaagccgaaa   1260
acggggtttg actcgtcagg ctttgtgcaa tatgtgtttc aatcgctcga tattacgctt   1320
ccgagaacgg taaaggaaca atcgactctt ggctcaggag acc                     1363

<210> 17
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of pga genomic region

<400> 17
aaagccttct cctctctatt                                                 20

<210> 18
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of pga genomic region

<400> 18
ttcttgaaaa agacaaggtc                                                 20

<210> 19
<211> 1027
<212> DNA
<213> Artificial Sequence

<220>
<223> homology regions: Fusion of 5- and 3-prime regions of the
      aprE gene with flanking BsaI sites

<400> 19
ggtctcgacc cgaagttctt ttttaacata taggtaaaac aatacgaaaa aaggcgccaa     60
gtattgaaga attgcagcag ccgcggcatt tcccttttcg attgaagcaa aaaacgtata    120
ttgaacagta agcattccaa aaatggaaaa tactaaaatc gaacaaatat ctgttttttt    180
cttccatatc tgacacacat gttgaaaacc gtttttcatt gaaacatata acaagagaat    240
gactcccgat gccagaagcc tgacagagac aagcgagccg gcttcaaccg ctcccctttc    300
aaatatgtac tgtgcagcgc ttcccgataa tccccacaat gaagcccctg caagcaccat    360
caatacgcct ttcacatgag ctgatttcat atctttcacc cgtttctgta tgcgatatat    420
tgcatatttt aatagatgat cgacaaggcc gcaacctcct tcggcaaaaa atgatctcat    480
aaaataaatg aatagtattt tcataaaatg agctcaataa catattctaa caaatagcat    540
atagaaaaag ctagtgtttt tagcactagc tttttcttca ttctgatgaa ggttgttcaa    600
tattttgaat ccgttccatg atcgtcggat ggccgtattt aaaaatcttg acgagaaacg    660
gcgggtttgc ctcgctcagc ccggcttttg agagctcttg aaacgtcgaa accgctgcat    720
cgctgttttg cgtcagttca atcgcatact ggtcagcagc tttttcctga tgcctcgaaa    780
ctgcgttcgt aaatggagac gacgcgaaag agatgacccc catcagcatc agaagaagcg    840
gaagtgcggc tagatcggat tttcctgcaa tatgaaggct tcttccatag cggccgatga    900
tccgcttgta cagcttgtcg atcacataaa agacagcaag ggataaaagc agatacccgc    960
caagtcctat gtaaacatgc ttcatcacat agtgccccat ttcgtgcgcc atgatgctca   1020
ggagacc                                                             1027

<210> 20
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of aprE genomic region

<400> 20
ccggttgtca ttgatccttt a                                               21

<210> 21
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of aprE genomic region

<400> 21
atcctcctgc aaaaaccgta t                                               21

<210> 22
<211> 41
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of pBW742

<400> 22
caaatttaca aaagcgactc gtgagttttc gttccactga g                         41

<210> 23
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of pBW742

<400> 23
gaacgttgct ctagagttaa gggattttgg tcatgg                               36

<210> 24
<211> 38
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of terminator region of
      pMutin2

<400> 24
gtggaacgaa aactcacgag tcgcttttgt aaatttgg                             38

<210> 25
<211> 40
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of terminator region of
      pMutin2

<400> 25
catgaccaaa atcccttaac tctagagcaa cgttcttgcc                           40

<210> 26
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> functional fragment: region of terminator region of
      pMutin2

<400> 26
ggggatctct gcagtgagat ct                                              22

<210> 27
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Sequence of terminator region in pCC009

<400> 27
ggggatctct gcagtcggga agat                                            24

<210> 28
<211> 128
<212> DNA
<213> Artificial Sequence

<220>
<223> promoter variant

<400> 28
aattttgtca aaataatttt attgacaacg tcttattaac gttgataccg gttaaatttt     60
atttgacaaa aatgggctcg tgttggagaa taaatgtgga gaaagattaa ctaataagga    120
ggacaaac                                                             128

<210> 29
<211> 128
<212> DNA
<213> Artificial Sequence

<220>
<223> promoter variant

<400> 29
aattttgtca aaataatttt attgacaacg tcttattaac gttgataccg gttaaatttt     60
atttgacaaa aatgggctcg tgttgaagaa taaatgtgga gaaagattaa ctaataagga    120
ggacaaac                                                             128

<210> 30
<211> 101
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide

<400> 30
aacacgagcc catttttgtc aaataaaatt taaccggtat caacgttaat aagacgttgt     60
caataaaatt attttgacaa aattttaata atccaaatga g                        101

<210> 31
<211> 87
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide

<400> 31
ctattgagta tttcttatcc atgtttgtcc tccttattag ttaatctttc tccacattta     60
ttctccaaca cgagcccatt tttgtca                                         87

<210> 32
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide

<400> 32
gattaactaa taaggaggac aaacatggat aagaaatact caataggc                  48

<210> 33
<211> 87
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide

<400> 33
ctattgagta tttcttatcc atgtttgtcc tccttattag ttaatctttc tccacattta     60
ttcttcaaca cgagcccatt tttgtca                                         87

<210> 34
<211> 127
<212> DNA
<213> Artificial Sequence

<220>
<223> promoter variant

<400> 34
aattttgtca aaataatttt attgacaacg tcttattaac gttgataccg gttaaatttt     60
atttgacaaa atgggctcgt gttggagaat aaatgtggag aaagattaac taataaggag    120
gacaaac                                                              127

<210> 35
<211> 126
<212> DNA
<213> Artificial Sequence

<220>
<223> promoter variant

<400> 35
aattttgtca aaataatttt attgacaacg tcttattaac gttgataccg gttaaatttt     60
atttgacaaa aatgggctcg tgttggagaa aatgtggaga aagattaact aataaggagg    120
acaaac                                                               126

<210> 36
<211> 127
<212> DNA
<213> Artificial Sequence

<220>
<223> promoter variant

<400> 36
aattttgtca aaataatttt attgacaacg tcttattaac gttgataccg gttaaatttt     60
atttgacaaa aatgggctcg tgttgggaat aaatgtggag aaagattaac taataaggag    120
gacaaac                                                              127

<210> 37
<211> 129
<212> DNA
<213> Artificial Sequence

<220>
<223> promoter variant

<400> 37
aattttgtca aaataatttt attgacaacg tcttattaac gttgataccg gttaaatttt     60
atttgaacaa aaatgggctc gtgttggaga ataaatgtgg agaaagatta actaataagg    120
aggacaaac                                                            129

<210> 38
<211> 127
<212> DNA
<213> Artificial Sequence

<220>
<223> promoter variant

<400> 38
aattttgtca aaataatttt attgacaacg tcttattaac gttgataccg gttaaatttt     60
atttgacaaa aatgggctcg tgttgagaat aaatgtggag aaagattaac taataaggag    120
gacaaac                                                              127

<210> 39
<211> 127
<212> DNA
<213> Artificial Sequence

<220>
<223> promoter variant

<400> 39
aattttgtca aaataatttt attgacaacg tcttattaac gttgataccg gttaaatttt     60
atttgacaaa aatgggctcg tgttggaaat aaatgtggag aaagattaac taataaggag    120
gacaaac                                                              127

<210> 40
<211> 127
<212> DNA
<213> Artificial Sequence

<220>
<223> promoter variant

<400> 40
aattttgtca aaataatttt attgacaacg tcttattaac gttgataccg gttaaatttt     60
atttgacaaa aagggctcgt gttggagaat aaatgtggag aaagattaac taataaggag    120
gacaaac                                                              127

<210> 41
<211> 127
<212> DNA
<213> Artificial Sequence

<220>
<223> promoter variant

<400> 41
aattttgtca aaataatttt attgacaacg tcttattaac gttgataccg gttaaatttt     60
atttgacaaa aatgggctcg tgttgaagaa aaatgtggag aaagattaac taataaggag    120
gacaaac                                                              127

<210> 42
<211> 130
<212> DNA
<213> Artificial Sequence

<220>
<223> promoter variant

<400> 42
aattttgtca aaataatttt attgacaacg tcttattaac cgttgatacc ggttaaattt     60
tatttgacaa aaatgggctc gtgttgaaga ataaatgtgg agaaagatta actaataagg    120
gaggacaaac                                                           130

<210> 43
<211> 127
<212> DNA
<213> Artificial Sequence

<220>
<223> promoter variant

<400> 43
aattttgtca aaataatttt attgacaacg tcttattaac gttgataccg gttaaatttt     60
atttgacaaa aatgggctcg tgttgaagaa taaatgtgga aaagattaac taataaggag    120
gacaaac                                                              127

<210> 44
<211> 128
<212> DNA
<213> Artificial Sequence

<220>
<223> promoter variant

<400> 44
aattttgtca aaataatttt attgacaacg tcttattaac gttgataccc ggttaaattt     60
tatttgacaa aaatgggctc gtgtgaagaa taaatgtgga gaaagattaa ctaataagga    120
ggacaaac                                                             128

<210> 45
<211> 127
<212> DNA
<213> Artificial Sequence

<220>
<223> promoter variant

<400> 45
aattttgtca aaataatttt attgacaacg tcttattaac gttgataccg gttaaatttt     60
atttgacaaa aatgggctcg tgttgagaat aaatgtggag aaagattaac taataaggag    120
gacaaac                                                              127

<210> 46
<211> 127
<212> DNA
<213> Artificial Sequence

<220>
<223> promoter variant

<400> 46
aattttgtca aaataatttt attgacaacg tcttattaac gttgataccg gttaaatttt     60
atttgacaaa atgggctcgt gttgaagaat aaatgtggag aaagattaac taataaggag    120
gacaaac                                                              127

<210> 47
<211> 129
<212> DNA
<213> Artificial Sequence

<220>
<223> promoter variant

<400> 47
aattttgtca aaataatttt attgacaacg tcttattaac gttgataccg gttaaatttt     60
attttgacaa aaatgggctc gtgttgaaga ataaatgtgg agaaagatta actaataagg    120
aggacaaac                                                            129

<210> 48
<211> 40
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide

<400> 48
gaagttttag atgccactct tatccatcaa tccatcactg                           40

<210> 49
<211> 41
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide

<400> 49
catctcaaat ttcgcattta ttccaatttc ctttttgcgt g                         41

<210> 50
<211> 40
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide

<400> 50
cacacgcaaa aaggaaattg gaataaatgc gaaatttgag                           40

<210> 51
<211> 40
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide

<400> 51
gaccagtgat ggattgatgg ataagagtgg catctaaaac                           40

<210> 52
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide

<400> 52
caagctatgc ttgctcaagc                                                 20

<210> 53
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide

<400> 53
ctgttggttc gcttgagcaa gcatagcttg gacggttcag cgtgttaagc                50

<210> 54
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide

<400> 54
ggtggtggtc tctaccccga tcgaagagcc attcgag                              37

<210> 55
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide

<400> 55
ggtggtggtc tcttgagctt cctctgtccg attgtcc                              37

<210> 56
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide

<400> 56
tacggcaatc tctgaaaaaa tgag                                            24

<210> 57
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide

<400> 57
aaacctcatt ttttcagaga ttgc                                            24

<210> 58
<211> 814
<212> DNA
<213> Artificial Sequence

<220>
<223> homology regions: Homology region of the mutated degU gene
      with flanking BsaI sites

<400> 58
ggtctcgacc cgatttggga ttgataccga cgctcagaaa atacttgaac acgatcgaag     60
attatcatgg aaaagcaaag attcatttcc aatgcatcgg agaatccgaa gaaagaagaa    120
tagcaccgcg gtttgaggtt gcactattcc ggcttgcaca ggaagcggtg acaaacgcct    180
taaaacactc cgaatcaact gaaattcatg ttaaagtaga agtgacaaaa gattttgtga    240
cgctgattat caaagacaat ggaaacggct ttgacttaaa agaagtaaaa ggcaagaaga    300
acaaatcttt cggtctgcta ggtatgaaag aaagagtcga tttgctcgaa ggctcaatga    360
caatcgattc gaaaataggt cttgggacat ttatattgat taaagttcca ctgtctttgt    420
aaagataatt gtaaaataga gacaaaagac atattgacca taaaagcggt gtgtttaaca    480
atgagaatgg ggaggcgtag cttgtgacta aagtaaatat tgtaattatt gacgatctgc    540
agttattccg tgaaggtgtc aaacggattt tggatttcga gcctaccttt gaagtagtgg    600
ccgaaggaga cgacggagat gaagcggctc gcattgtcga gcactaccat cctgatgttg    660
ttatcatgga tattaatatg ccgaatgtga acggagtaga agcgacaaaa caactggtcg    720
acttgtatcc ggaatcaaag gttattattt tatccatcca tgatgacgaa aactatgtta    780
cacatgcatt aaaaacagga gccctcagga gacc                                814

<210> 59
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide

<400> 59
tacgggattt cgaacctacc tttg                                            24

<210> 60
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide

<400> 60
aaaccaaagg taggttcgaa atcc                                            24

<210> 61
<211> 39
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide

<400> 61
ggttgcggtc tcatacgtga agatcaggct atcactggt                            39

<210> 62
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide

<400> 62
caacgggtct cttgagttgg caggccgctg aatttc                               36

<210> 63
<211> 1435
<212> DNA
<213> Artificial Sequence

<220>
<223> sgRNA and homology regions: protospacer aprE-sgRNA,
      homology region of the aprE gene with flanking BsaI sites

<400> 63
ggttgcggtc tcatacggaa acaaacccat accaggagtt ttagagctag aaatagcaag     60
ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgctttttac    120
tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatc taaagcttag    180
gcccagtcga aagactgggc ctttttaata cgactcacta tagggtcgac ggccaacgag    240
gcccattttc ttctgctatc aaaataacag actcgtgatt ttccaaacga gctttcaaaa    300
aagcctctgc cccttgcaaa tcggatgcct gtctataaaa ttcccgatat tggttaaaca    360
gcggcgcaat ggcggccgca tctgatgtct ttgcttggcg aatgttcatc ttatttcttc    420
ctccctctca ataatttttt cattctatcc cttttctgta aagtttattt ttcagaatac    480
ttttatcatc atgctttgaa aaaatatcac gataatatcc attgttctca cggaagcaca    540
cgcaggtcat ttgaacgaat tttttcgaca ggaatttgcc gggactcagg agcatttaac    600
ctaaaaaagc atgacatttc agcataatga acatttactc atgtctattt tcgttctttt    660
ctgtatgaaa atagttattt cgagtctcta cggaaatagc gagagatgat atacctaaat    720
agagataaaa tcatctcaaa aaaatgggtc tactaaaata ttattccatc tattacaata    780
aattcacaga atagtctttt aagtaagtct actctgaatt tttttaaaag gagagggtaa    840
agataatagt aaaaagaagc aggttcctcc atacctgctt ctttttattt gtcagcatcc    900
tgatgttccg gcgcattctc ttctttctcc gcatgttgaa tccgttccat gatcgacgga    960
tggctgcctc tgaaaatctt cacaagcacc ggaggatcaa cctggctcag ccccgtcacg   1020
gccaaatcct gaaacgtttt aacagcggct tctctgttct ctgtcaactc gatcccatac   1080
tggtcagcct tattctcctg ataacgcgag acagcattag aaaaaggcgt aaccgcaaag   1140
ctcaaaacag aaaacaaaag caataacagc ggaagtgccg caagatcatg ccgcccttct   1200
aaatgaaaca tgctgcgggt taggcgaacc gtccgcttgt aaagcttatc aatgacataa   1260
aatccggcga gcgacacgag caaatagcca gccagaccga tgtaaacgtg cttcatgaca   1320
taatggccca tttcgtggcc cataataaac agaatttctg aatcgtcaag tttgttcagc   1380
gtcgtatccc acaatacaat ccgtttattg gccccaattc tcaagagacc cgttg        1435

<210> 64
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Protospacer: protospacer target sequence for aprE gene

<400> 64
gaaacaaacc cataccagga                                                 20

<210> 65
<211> 1241
<212> DNA
<213> Artificial Sequence

<220>
<223> sgRNA and homology regions: protospacer vpr-sgRNA,
      homology region of the vpr gene with flanking BsaI sites

<400> 65
ggttgcggtc tcatacgatc aggaacggaa cgagtcggtt ttagagctag aaatagcaag     60
ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgctttttac    120
tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatc taaactagta    180
cagtcgatac ggatccacta gtctatctct tctctttttt ccgaaaagcc gcctgcctga    240
taagcatcgg cagcctgata tgtaccgccg gcgaacgccg tcaccttcat ctgattttct    300
ctgacaggca tcaccgcatt cagcagcacg gctgtccata attttaaaaa tgatatcaaa    360
cctttcatac cgatccctcc agtttcgttt tgataaaact agcaactcta ttaaactttc    420
ttgctctatc ttatcccagc aaaatgaaaa tgtttgtcac aatgtgtgtg caaaatgatt    480
ctagttttta gaagttttgt tgaaaactga aggaatcgca tgattcagcg gatacaaacc    540
atgaatgtaa cttactcaca gcttatccta aggataaaca catattaccc acaggatata    600
tccacatatc cacatactta ttcaatattt agtataagaa cgtatattcc ctacaatatc    660
tatacacaag tttattcact tatacacagt aaattgtgca taaatctaat gacaagcctt    720
gttgagaacc actcaacaag gcttttttat gttaaaatac ggataatgcg ttcaggagaa    780
gctccccttc tcttcaaaac gtgaaaaaag caatcggagg acatcgtgta tatgctttct    840
tttatcgtat tattcggctt atccttcatt attgtctgct ttatattttt cacgactttg    900
tacttcgccg tcaacctgca gaagcgcgag cccaagcctt ttcaaaaagc tgcggagcaa    960
accgtcgata ccatcatcct cattccgctc agctggctgt ttaccgcttt atacatatgc   1020
attctgttta ttcttttccc aatccgccat tttctcgatt tttttcagca aaaacgctaa   1080
attgactgat gaaacgcttc ggccagcagc cggtatgaat ccaatctgtc ttgaaaatcg   1140
tgggtgatcg tcaccgccat gatttcgtcc gttccgtaag cgccggccag ttcaagcagc   1200
tgttcctagc tcgagccatg gctcactcaa gagacccgtt g                       1241

<210> 66
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Protospacer: protospacer target sequence for vpr gene

<400> 66
atcaggaacg gaacgagtcg                                                 20

<210> 67
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Protospacer: protospacer target sequence for vpr gene

<400> 67
gcttccgtat aatgagtatt                                                 20

<210> 68
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Protospacer: protospacer target sequence for vpr gene

<400> 68
cacgatccga aaaacccgta                                                 20

<210> 69
<211> 1338
<212> DNA
<213> Artificial Sequence

<220>
<223> sgRNA and homology regions: protospacer epr-sgRNA,
      homology region of the epr gene with flanking BsaI sites

<400> 69
ggttgcggtc tcatacgatt ccggtccagc gcttttagtt ttagagctag aaatagcaag     60
ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgctttttac    120
tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatc taaactagta    180
ggcccagtcg atacgtaaag actgggcctt tttaatacga ctcactatag ggtcgacggc    240
caacgaggcc tcgaggatcc gatatcatgc atggcgcgcc accctgagac gaccctgatg    300
caggtgaccc ggatccacta gtccgatttg acatcgtgct gtttaaagga cctgacaaag    360
atatattcat taaaagggtg atcgggcttc cgggcgaaac cctcaggtat gaagatgatc    420
agctgtatat caacgaagaa aagatcaaag agccttatct ggacgactta aaggccgtca    480
ccgccggagg ggacttgaca ggggatttta cactgcagga agtgaccgga gaggagaagg    540
tgcctgaaaa cgagtacttc gtcctcgggg acaaccggat ccacagcttt gacagccgcc    600
atttcggctt tgtttcagaa cgggacatcg tcgggattgt gacggaaaga attgataaga    660
agtgattgga gagtacgggg gagagtaagc ggccgaccaa ggaatacgat tacgcaaatg    720
acgagcccga aatgtcaatt agtacaacag catcaataat gacgcatttg ctaaatatga    780
aaattaaaag gcccggatga ttccgggctt ttttccgtac taagcggcgt tcgctatata    840
tatcggagga tttttgaatt ttcaaagaga aagaaagctt atcttaaggt cgcttgtcat    900
gcacctttag tttttaaaac gttaaaaaca ctgttatatc aacatttgtg aagcttcctg    960
tttattcggg aagttaaatt gggtactcca agttagtttt aaaaaagagt cataaggcca   1020
gcttctatcg atgaatcatt tttaagcgac gccttttgtc taaaatgtat aatgttactt   1080
ttgtttttgt tgaaagtgaa caatgttatt gactggctta caacctacaa tttaattaaa   1140
taaaaaatag attaaaagaa gggagcgttc tcataccgtg gaaaaaacaa ttaaacatga   1200
ccccaattat tacaaaaaga taattattgc attgtgttta gggtgggtcg ctatttggat   1260
ttatcgtaca atacttacgc caatatatcc gcagattcaa gaatcattag ggaatattag   1320
tgctcaagag acccgttg                                                 1338

<210> 70
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Protospacer: protospacer target sequence for epr gene

<400> 70
attccggtcc agcgctttta                                                 20

<210> 71
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Protospacer: protospacer target sequence for epr gene

<400> 71
cgctttttca gctttggcaa                                                 20

<210> 72
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Protospacer: protospacer target sequence for epr gene

<400> 72
gcaaacatgc cggtgacgtg                                                 20

<210> 73
<211> 865
<212> DNA
<213> Artificial Sequence

<220>
<223> functional fragment: T2A lacZ cassette

<400> 73
gcgatgttaa ggaccgtctc atctcacctg caaggtctca tctcttttac tccatctgga     60
tttgttcaga acgctcggtt gccgccgggc gttttttatc taaaactagt gtcgagggtc    120
ttcggtaccg cgatttacat atgctggcac gacaggtttc ccgactggaa agcgggcagt    180
gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt    240
atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac    300
agctatgacc atgattacgc caagcttgca tgcctgcagg tcgactctag aggatccccg    360
ggtaccgagc tcgaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg    420
cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga    480
agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct    540
gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct    600
cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc    660
tgccgcgttt ataatgaaga ccctagcagg catcaaataa aacgaaaggc tcagtcgaaa    720
gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat    780
ccgccgccct agacagctgt cgctgagacg atcgctgaga cctcgcaagt tctcgccatc    840
gcaggtgaaa tctagatgta ttcgc                                          865

<210> 74
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of promoter PaprE

<400> 74
tatatgaaga ccttcgactc gggacctctt tccctcg                              37

<210> 75
<211> 41
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of promoter PaprE

<400> 75
tatagaagac ttatatctca ctctcctcct ctttattcag a                         41

<210> 76
<211> 747
<212> DNA
<213> Artificial Sequence

<220>
<223> functional fragment: GFPmut2 AF302837 with flanking BpiI
      restriction sites

<400> 76
attagaagac ctatatgagt aaaggagaag aacttttcac tggagttgtc ccaattcttg     60
ttgaattaga tggcgatgtt aatgggcaaa aattctctgt cagtggagag ggtgaaggtg    120
atgcaacata cggaaaactt acccttaaat ttatttgcac tactgggaag ctacctgttc    180
catggccaac acttgtcact actttcgcgt atggtcttca atgctttgcg agatacccag    240
atcatatgaa acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa    300
gaactatatt ttacaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag    360
gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca    420
ttcttggaca caaaatggaa tacaactata actcacataa tgtatacatc atggcagaca    480
aaccaaagaa tggaatcaaa gttaacttca aaattagaca caacattaaa gatggaagcg    540
ttcaattagc agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac    600
cagacaacca ttacctgtcc acacaatctg ccctttccaa agatcccaac gaaaagagag    660
atcacatgat ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac    720
tatacaaata atagcttgtc ttcatta                                        747

<210> 77
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of amyB genomic region

<400> 77
tataggtctc aacccataat gccgtcgcac tgg                                  33

<210> 78
<211> 35
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of amyB genomic region

<400> 78
ccttggtctc gtcgctagaa gagcagagag gacgg                                35

<210> 79
<211> 39
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of amyB genomic region

<400> 79
ttgagaggtc tcagagacat tttccctata ttttcttcc                            39

<210> 80
<211> 35
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of amyB genomic region

<400> 80
gtgaggtctc atgagaccat ccgttattga caagg                                35

<210> 81
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Protospacer: protospacer target sequence for sigE gene

<400> 81
cggtgaggaa aaaacccaaa                                                 20

<210> 82
<211> 1338
<212> DNA
<213> Artificial Sequence

<220>
<223> sgRNA and homology regions: protospacer epr-sgRNA,
      homology region of the sigE gene with flanking BsaI sites

<400> 82
ggttgcggtc tcatacgcgg tgaggaaaaa acccaaagtt ttagagctag aaatagcaag     60
ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgctttttac    120
tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatc taaactagta    180
ggcccagtcg atacgtaaag actgggcctt tttaatacga ctcactatag ggtcgacggc    240
caacgaggcc tcgaggatcc gatatcatgc atggcgcgcc accctgagac gaccctgatg    300
caggtgaccc ggatccacta gtaagacagt atgatgaaca agtgcttgtg gaactacaca    360
ttcacggaga gacaattcgt ttaaagggac tcgtcgattc tggcaaccag ctgtatgatc    420
ctatgaccaa aacaccggtc atgatcgtcc aggccgacca tctaacggcc atttgcggag    480
aatcgtttat agaccttatg aaacagtctc atcctgttga agtcatgcaa aagatcgatg    540
atcaatttcc tcttcttgat cgattaagac ttgttccata tcgagcagtc ggtcatgatc    600
acggttttct actatgccta aaaccagata cagttgtcat ttattcaaag acgcatatga    660
ttcagccagc taagtgtttt gtaggattga gtctgagcgg cttatcggca gatcaggaat    720
ttcaatccat cattcatcca gatatgttag acgggaaaat catccagggg gtgtcgtagt    780
ttttggtgat gtcttttatc ttacgaggtc aacttgacat tttgaaaaat ttttttgaaa    840
agctctgtcc ctgtactgtc aaaggaaaca accttttttc tcatgattct cgtcatcgct    900
cgtgcatatt tttccaaccc aaggagatac tgaactttgt acaacagctc ctgtagggag    960
ggaaaaaagt gtccagaaat aaagtggaaa tctgcggagt cgacacctcc aagctgcctg   1020
ttctgaaaaa cgacgagatg agaaaattgt tcagacagct gcaagatgag ggtgacgata   1080
cagcaagaga aaagctagtc aatggcaatt tacggttggt tttaagtgtg attcagcgtt   1140
tcaacaacag aggcgagtat gtcgatgatc tctttcaagt aggctgtatc ggattaatga   1200
aatcaattga taattttgat ttaagccaca atgtcagatt ttcaacttat gcggttccta   1260
tgatcatagg agaaatccgt cgatacttgc gtgataataa tccgattcgg gtgtctcgct   1320
cactcaagag acccgttg                                                 1338

<210> 83
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Protospacer: protospacer target sequence for sigF gene

<400> 83
tgtcgtcctc gctcaagaag                                                 20

<210> 84
<211> 1338
<212> DNA
<213> Artificial Sequence

<220>
<223> sgRNA and homology regions: protospacer epr-sgRNA,
      homology region of the sigF gene with flanking BsaI sites

<400> 84
ggttgcggtc tcatacgtgt cgtcctcgct caagaaggtt ttagagctag aaatagcaag     60
ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgctttttac    120
tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatc taaactagta    180
ggcccagtcg atacgtaaag actgggcctt tttaatacga ctcactatag ggtcgacggc    240
caacgaggcc tcgaggatcc gatatcatgc atggcgcgcc accctgagac gaccctgatg    300
caggtgaccc ggatccacta gtaaattatc cgtatggagc cttcagagca aacggcattg    360
caaacattgg gggtggcatc atgaggaatg aaatgaacct gaccttctct gccttaagtc    420
aaaatgaatc ctttgcgagg gtgacagtcg cggcgtttat cgcccagctt gatccgacat    480
tagatgaatt aactgaaatc aaaaccgttg tgtcagaggc ggtgacaaac tccattatcc    540
atggctatga tgggaatcca gatggcaagg tgcatattga agtcacactt gatgatcatg    600
ttgtgtacct gaccatccgt gacgaaggaa tgggtattac agatcttgag gaagcaagac    660
agccgctttt cacgacaaaa ccagacttag aacgctctgg catgggcttt accattatgg    720
agaattttat ggatgatgtc atgatagact catctccaga aatgggcaca accatccgtt    780
taacaaagca tctatcaaaa agcaaagcgc tttgtaatta aatagccaat tcggctggct    840
ttttttgtgt ggtaattacc ggtaaatgaa gttctctcgg tatgagaacc atttttcacc    900
acatactatt tttaacccat cgtataggaa gtgacttgga tggacggaca aatctttctt    960
cggctgcgcc atcgcattaa gaccggtaat gatcagctca tttatttaga agatatcgcc   1020
caaatcactg gtgatgagtt ggctgtgcaa aagcttagca agatgccgat atatcatgtc   1080
agtaaaaagg atcgtcacat tgccgttctt gatatcatgc atgtggtcaa aacgatcaaa   1140
aaaacatggc caaccatcga cattcaaact gtcggaggcg ctgaagccat tgttgaaatt   1200
gatacaggca aacgccagct ttctcccgta ttatttgtgt tcgtgtggct tttattattt   1260
gtcggagcgg cgcttgccat tatgaatttc cacgaggatg tcagtatgcg gctcgtccat   1320
atctcaagag acccgttg                                                 1338

<210> 85
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Protospacer: protospacer target sequence for spoIIE gene

<400> 85
cttgtagctg aacagctgat                                                 20

<210> 86
<211> 1338
<212> DNA
<213> Artificial Sequence

<220>
<223> sgRNA and homology regions: protospacer epr-sgRNA,
      homology region of the spoIIE gene with flanking BsaI sites

<400> 86
ggttgcggtc tcatacgctt gtagctgaac agctgatgtt ttagagctag aaatagcaag     60
ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgctttttac    120
tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatc taaactagta    180
ggcccagtcg atacgtaaag actgggcctt tttaatacga ctcactatag ggtcgacggc    240
caacgaggcc tcgaggatcc gatatcatgc atggcgcgcc accctgagac gaccctgatg    300
caggtgaccc ggatccacta gtcctgctgt ccgcaggtgc tttttttctt gacccacacg    360
acattttttg agatttcgtc atttaattta aaacttccta ttgacggaca agcgattcct    420
ttgtattata gatcttgtgc ttcttagcgc atttttatta tggcggtgta gctcagctgg    480
ctagagcgta cggttcatac ccgtgaggtc gggggttcga tcccctccgc cgctatcctt    540
ttgattagaa cataaaagca aggcccgttg gtcaagcggt taagacaccg ccctttcacg    600
gcggtaacac gggttcgaat cccgtacggg tcatcttcga aaacagcttt ctttaggaaa    660
gctgtttttt tgtgtcttca taaaattctg atgaaagaca tcgactttca agaaagtatg    720
cctctttgac gaataaagcg tcgaacgttt tatggaaacg acaacttctt ttgacaaaat    780
ttctttttca ccttcgctat aatgacaagc aacgaatatc agtgaaatat cgtataatat    840
gaatttcttc tggcgatgat ggggatataa agcattcagt acgatcccag gaggaatgaa    900
gatgcgaaaa ggtcacgtaa accaaatctt attgattaca gatggctgct caaatcacgg    960
ggaagatcca cttgcgattg cctcattggc aaaggaacaa gggattacag tcaatgttat   1020
tggcattatg gaggaaaaca gacacgacca tgaagcaatg aaagaagttg aagggattgc   1080
tctcgcaggt ggaggcatcc atcaagttgt ctacgtccag cagttatctc aaaccgtaca   1140
aatggttaca aaaaaagcga tgacacaaac cttgcaaggt gttgtgaata aagaattgca   1200
gcaaatactt ggcaaggaca ctgaaattga agagctgcca cctgataaac gcggggaagt   1260
gatggaagta gtcgatgagt taggagagac ggttcatctt caagtgcttg tgcttgttga   1320
tactcaagag acccgttg                                                 1338

<210> 87
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of hag genomic region

<400> 87
tgatcttgat gaaacgacgg                                                 20

<210> 88
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of hag genomic region

<400> 88
taatcctgat attctgatcg cc                                              22

<210> 89
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of degU genomic region

<400> 89
atgatttaag gccgatggc                                                  19

<210> 90
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of degU genomic region

<400> 90
atccgccttc agctactact t                                               21

<210> 91
<211> 26
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of amyE genomic region

<400> 91
ggtcatgaat aatctgcgta atagac                                          26

<210> 92
<211> 26
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of amyE genomic region

<400> 92
gcgtgtacgt tttgaggcgc tgcgcc                                          26

<210> 93
<211> 26
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of aprE genomic region
      B. subtilis

<400> 93
gagctggcag atgaagccaa tattcc                                          26

<210> 94
<211> 27
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of aprE genomic region
      B. subtilis

<400> 94
gtacgcgcat gaggaacgac aaataag                                         27

<210> 95
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of vpr genomic region

<400> 95
ctgtcaccca cttcccatta tgag                                            24

<210> 96
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of vpr genomic region

<400> 96
gtgaccgaag gctttccatc attg                                            24

<210> 97
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of epr genomic region

<400> 97
cttgtcatcg tcgtcgggat tcag                                            24

<210> 98
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of epr genomic region

<400> 98
gtgccaatca caaatgtagc cagc                                            24

<210> 99
<211> 25
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of sigE genomic region

<400> 99
gaggaggcat gatcggagtt cattc                                           25

<210> 100
<211> 25
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of sigE genomic region

<400> 100
ctcctccgtc attatagatc ggttc                                           25

<210> 101
<211> 25
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of sigF genomic region

<400> 101
gtgacgaatt atttggaaac agagg                                           25

<210> 102
<211> 25
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of sigF genomic region

<400> 102
cgacataatg atcgagatcg agctg                                           25

<210> 103
<211> 25
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of spoIIE genomic
      region

<400> 103
ctcaacaaca acaatcaaga ccgag                                           25

<210> 104
<211> 25
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of spoIIE genomic
      region

<400> 104
gatagtgaat cgaatcgagg cgtcc                                           25
