                         SEQUENCE LISTING

<110>  SORRENTO THERAPEUTICS, INC.
 
<120>  Dimeric Antigen Receptors (DAR) that Bind CD20

<130>  01223-0068-00PCT

<140>  PCT/US21/20089
<141>  2021-02-26

<150>  US 62/982,348
<151>  2020-02-27

<150>  US 63/089,869
<151>  2020-10-09

<160>  62    

<170>  PatentIn version 3.5

<210>  1
<211>  297
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Thr Thr Pro Arg Asn Ser Val Asn Gly Thr Phe Pro Ala Glu Pro 
1               5                   10                  15      


Met Lys Gly Pro Ile Ala Met Gln Ser Gly Pro Lys Pro Leu Phe Arg 
            20                  25                  30          


Arg Met Ser Ser Leu Val Gly Pro Thr Gln Ser Phe Phe Met Arg Glu 
        35                  40                  45              


Ser Lys Thr Leu Gly Ala Val Gln Ile Met Asn Gly Leu Phe His Ile 
    50                  55                  60                  


Ala Leu Gly Gly Leu Leu Met Ile Pro Ala Gly Ile Tyr Ala Pro Ile 
65                  70                  75                  80  


Cys Val Thr Val Trp Tyr Pro Leu Trp Gly Gly Ile Met Tyr Ile Ile 
                85                  90                  95      


Ser Gly Ser Leu Leu Ala Ala Thr Glu Lys Asn Ser Arg Lys Cys Leu 
            100                 105                 110         


Val Lys Gly Lys Met Ile Met Asn Ser Leu Ser Leu Phe Ala Ala Ile 
        115                 120                 125             


Ser Gly Met Ile Leu Ser Ile Met Asp Ile Leu Asn Ile Lys Ile Ser 
    130                 135                 140                 


His Phe Leu Lys Met Glu Ser Leu Asn Phe Ile Arg Ala His Thr Pro 
145                 150                 155                 160 


Tyr Ile Asn Ile Tyr Asn Cys Glu Pro Ala Asn Pro Ser Glu Lys Asn 
                165                 170                 175     


Ser Pro Ser Thr Gln Tyr Cys Tyr Ser Ile Gln Ser Leu Phe Leu Gly 
            180                 185                 190         


Ile Leu Ser Val Met Leu Ile Phe Ala Phe Phe Gln Glu Leu Val Ile 
        195                 200                 205             


Ala Gly Ile Val Glu Asn Glu Trp Lys Arg Thr Cys Ser Arg Pro Lys 
    210                 215                 220                 


Ser Asn Ile Val Leu Leu Ser Ala Glu Glu Lys Lys Glu Gln Thr Ile 
225                 230                 235                 240 


Glu Ile Lys Glu Glu Val Val Gly Leu Thr Glu Thr Ser Ser Gln Pro 
                245                 250                 255     


Lys Asn Glu Glu Asp Ile Glu Ile Ile Pro Ile Gln Glu Glu Glu Glu 
            260                 265                 270         


Glu Glu Thr Glu Thr Asn Phe Pro Glu Pro Pro Gln Asp Gln Glu Ser 
        275                 280                 285             


Ser Pro Ile Glu Asn Asp Ser Ser Pro 
    290                 295         


<210>  2
<211>  19
<212>  PRT
<213>  Mus musculus

<400>  2

Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      


Val His Ser 
            


<210>  3
<211>  121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-CD20 heavy chain variable region

<400>  3

Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Asn Met His Trp Val Lys Gln Thr Pro Gly Arg Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ser Thr Tyr Tyr Gly Gly Asp Trp Tyr Phe Asn Val Trp Gly 
            100                 105                 110         


Ala Gly Thr Thr Val Thr Val Ser Ala 
        115                 120     


<210>  4
<211>  108
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-CD20 heavy chain constant region (CH1)

<400>  4

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr 
            100                 105             


<210>  5
<211>  42
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 hinge

<400>  5

Pro Arg Lys Ile Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu 
1               5                   10                  15      


Lys Ser Asn Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro 
            20                  25                  30          


Ser Pro Leu Phe Pro Gly Pro Ser Lys Pro 
        35                  40          


<210>  6
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 transmembrane domain

<400>  6

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
            20                  25          


<210>  7
<211>  42
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  4-1BB intracellular costimulatory sequence

<400>  7

Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40          


<210>  8
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD3zeta intracellular signaling region, ITAM3 only

<400>  8

Arg Val Lys Phe Ser Arg Ser Ala Asp Lys Gly Glu Arg Arg Arg Gly 
1               5                   10                  15      


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
            20                  25                  30          


Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
        35                  40                  45  


<210>  9
<211>  21
<212>  PRT
<213>  Thosea asigna virus

<400>  9

Gly Ser Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu 
1               5                   10                  15      


Glu Asn Pro Gly Pro 
            20      


<210>  10
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Light chain leader peptide

<400>  10

Met Ser Val Pro Thr Gln Val Leu Gly Leu Leu Leu Leu Trp Leu Thr 
1               5                   10                  15      


Asp Ala Arg Cys 
            20  


<210>  11
<211>  106
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-CD20 light chain variable region

<400>  11

Gln Ile Val Leu Ser Gln Ser Pro Ala Ile Leu Ser Ala Ser Pro Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Thr Cys Arg Ala Ser Ser Ser Val Ser Tyr Ile 
            20                  25                  30          


His Trp Phe Gln Gln Lys Pro Gly Ser Ser Pro Lys Pro Trp Ile Tyr 
        35                  40                  45              


Ala Thr Ser Asn Leu Ala Ser Gly Val Pro Val Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Arg Val Glu Ala Glu 
65                  70                  75                  80  


Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Thr Ser Asn Pro Pro Thr 
                85                  90                  95      


Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105     


<210>  12
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-CD20 light chain constant region (kappa)

<400>  12

Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
1               5                   10                  15      


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
            20                  25                  30          


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
        35                  40                  45              


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
    50                  55                  60                  


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
65                  70                  75                  80  


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
                85                  90                  95      


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
            100                 105         


<210>  13
<211>  658
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-CD20 DAR precursor polypeptide

<400>  13

Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      


Val His Ser Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Val Lys 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Ser Tyr Asn Met His Trp Val Lys Gln Thr Pro Gly Arg Gly Leu 
    50                  55                  60                  


Glu Trp Ile Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Arg Ser Thr Tyr Tyr Gly Gly Asp Trp Tyr Phe Asn 
        115                 120                 125             


Val Trp Gly Ala Gly Thr Thr Val Thr Val Ser Ala Ala Ser Thr Lys 
    130                 135                 140                 


Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly 
145                 150                 155                 160 


Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro 
                165                 170                 175     


Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr 
            180                 185                 190         


Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val 
        195                 200                 205             


Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn 
    210                 215                 220                 


Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg Val Glu Pro 
225                 230                 235                 240 


Lys Ser Cys Asp Lys Thr His Thr Pro Arg Lys Ile Glu Val Met Tyr 
                245                 250                 255     


Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn Gly Thr Ile Ile His 
            260                 265                 270         


Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu Phe Pro Gly Pro Ser 
        275                 280                 285             


Lys Pro Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr 
    290                 295                 300                 


Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Lys Arg Gly 
305                 310                 315                 320 


Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val 
                325                 330                 335     


Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu 
            340                 345                 350         


Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp 
        355                 360                 365             


Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly 
    370                 375                 380                 


Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala 
385                 390                 395                 400 


Leu Pro Pro Arg Gly Ser Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys 
                405                 410                 415     


Gly Asp Val Glu Glu Asn Pro Gly Pro Met Ser Val Pro Thr Gln Val 
            420                 425                 430         


Leu Gly Leu Leu Leu Leu Trp Leu Thr Asp Ala Arg Cys Gln Ile Val 
        435                 440                 445             


Leu Ser Gln Ser Pro Ala Ile Leu Ser Ala Ser Pro Gly Glu Lys Val 
    450                 455                 460                 


Thr Met Thr Cys Arg Ala Ser Ser Ser Val Ser Tyr Ile His Trp Phe 
465                 470                 475                 480 


Gln Gln Lys Pro Gly Ser Ser Pro Lys Pro Trp Ile Tyr Ala Thr Ser 
                485                 490                 495     


Asn Leu Ala Ser Gly Val Pro Val Arg Phe Ser Gly Ser Gly Ser Gly 
            500                 505                 510         


Thr Ser Tyr Ser Leu Thr Ile Ser Arg Val Glu Ala Glu Asp Ala Ala 
        515                 520                 525             


Thr Tyr Tyr Cys Gln Gln Trp Thr Ser Asn Pro Pro Thr Phe Gly Gly 
    530                 535                 540                 


Gly Thr Lys Leu Glu Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe 
545                 550                 555                 560 


Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser Val 
                565                 570                 575     


Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp 
            580                 585                 590         


Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu Ser Val Thr 
        595                 600                 605             


Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr 
    610                 615                 620                 


Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr Ala Cys Glu Val 
625                 630                 635                 640 


Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser Phe Asn Arg Gly 
                645                 650                 655     


Glu Cys 
        


<210>  14
<211>  385
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-CD20 DAR 1st polypeptide chain

<400>  14

Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Asn Met His Trp Val Lys Gln Thr Pro Gly Arg Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ser Thr Tyr Tyr Gly Gly Asp Trp Tyr Phe Asn Val Trp Gly 
            100                 105                 110         


Ala Gly Thr Thr Val Thr Val Ser Ala Ala Ser Thr Lys Gly Pro Ser 
        115                 120                 125             


Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala 
    130                 135                 140                 


Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val 
145                 150                 155                 160 


Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala 
                165                 170                 175     


Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val 
            180                 185                 190         


Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His 
        195                 200                 205             


Lys Pro Ser Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys 
    210                 215                 220                 


Asp Lys Thr His Thr Pro Arg Lys Ile Glu Val Met Tyr Pro Pro Pro 
225                 230                 235                 240 


Tyr Leu Asp Asn Glu Lys Ser Asn Gly Thr Ile Ile His Val Lys Gly 
                245                 250                 255     


Lys His Leu Cys Pro Ser Pro Leu Phe Pro Gly Pro Ser Lys Pro Phe 
            260                 265                 270         


Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu 
        275                 280                 285             


Val Thr Val Ala Phe Ile Ile Phe Trp Val Lys Arg Gly Arg Lys Lys 
    290                 295                 300                 


Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr 
305                 310                 315                 320 


Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly 
                325                 330                 335     


Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Lys Gly Glu 
            340                 345                 350         


Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
        355                 360                 365             


Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro 
    370                 375                 380                 


Arg 
385 


<210>  15
<211>  213
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-CD20 DAR 2nd polypeptide chain

<400>  15

Gln Ile Val Leu Ser Gln Ser Pro Ala Ile Leu Ser Ala Ser Pro Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Thr Cys Arg Ala Ser Ser Ser Val Ser Tyr Ile 
            20                  25                  30          


His Trp Phe Gln Gln Lys Pro Gly Ser Ser Pro Lys Pro Trp Ile Tyr 
        35                  40                  45              


Ala Thr Ser Asn Leu Ala Ser Gly Val Pro Val Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Arg Val Glu Ala Glu 
65                  70                  75                  80  


Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Thr Ser Asn Pro Pro Thr 
                85                  90                  95      


Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Thr Val Ala Ala Pro 
            100                 105                 110         


Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly Thr 
        115                 120                 125             


Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys 
    130                 135                 140                 


Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu 
145                 150                 155                 160 


Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser 
                165                 170                 175     


Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr Ala 
            180                 185                 190         


Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser Phe 
        195                 200                 205             


Asn Arg Gly Glu Cys 
    210             


<210>  16
<211>  1974
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence encoding the anti-CD20 DAR

<400>  16
atggagtgga gctgggtgtt tctgttcttc ctctccgtca caaccggcgt gcatagccaa       60

gtgcaattgc agcagcccgg tgccgaactc gtgaaaccag gagcaagcgt aaagatgtcc      120

tgtaaggcat caggttatac ctttaccagc tacaacatgc actgggtgaa acaaacgccg      180

gggcggggcc tcgaatggat aggcgcgata tatcccggaa atggcgatac cagttacaat      240

cagaagttca aaggcaaagc gacactgaca gctgataagt cttcaagcac cgcctatatg      300

caactttcta gcctgaccag cgaagactcc gccgtttatt actgtgctcg gtccacatac      360

tacggaggcg attggtactt taatgtgtgg ggtgcgggca ccactgtcac tgtatcagcg      420

gcttccacca agggcccctc cgtgttccct ctggccccca gcagcaagag cacatccgga      480

ggcaccgccg ccctcggatg tctggtgaag gactacttcc ccgagcctgt caccgtgtcc      540

tggaatagcg gcgccctcac ctccggcgtg cacaccttcc ccgctgtcct gcagtcctcc      600

ggactgtaca gcctgtcctc cgtcgtgacc gtgcctagct cctccctcgg cacccagacc      660

tacatctgca acgtgaacca caagccttcc aacacaaagg tggacaaacg ggtggagccc      720

aagtcctgcg acaaaaccca cacccccaga aagatagagg tgatgtaccc tcccccctac      780

ttggacaacg aaaagtctaa tggcactatc attcacgtaa agggcaaaca cctttgtcca      840

agtcctttgt tcccaggccc atctaagccg ttctgggtac tcgtggttgt ggggggcgtg      900

ctcgcttgtt actcactgct ggtgacggtg gcctttatta ttttctgggt taaacggggc      960

agaaagaaac tcctgtatat attcaaacaa ccatttatga gaccagtaca aactactcaa     1020

gaggaagatg gctgtagctg ccgatttcca gaagaggagg aaggaggatg tgaactgagg     1080

gtaaaattta gcaggtctgc agataaaggg gagaggagac gcgggaaggg ccatgatgga     1140

ctgtatcagg gactttccac agccaccaag gacacctatg acgctctcca catgcaggcc     1200

ctgccccctc gcggaagcgg agagggcaga ggaagtctgc taacatgcgg tgacgtcgag     1260

gagaatcctg gacctatgtc cgtccctacc caggtgctgg gcctgctgct gctgtggctg     1320

accgatgcta gatgccagat agtcctgagc caatcaccgg ccatcttgtc tgcctctcct     1380

ggcgaaaagg tgacgatgac ttgcagagcc agtagctctg taagctatat acactggttc     1440

cagcaaaaac cgggctcttc tccgaagccg tggatatacg caacttcaaa cctggcgtct     1500

ggggttcctg taaggtttag cggcagcggt tcaggcacga gctacagcct tactatctcc     1560

cgggttgagg ctgaagatgc agccacatac tactgtcagc agtggacttc aaatccacct     1620

acattcgggg gaggcacgaa gctggagatt aaacgaaccg ttgcggcgcc tagtgtgttc     1680

atattcccgc cgtctgatga acaactcaag tctggaacgg caagtgtggt gtgtctcctg     1740

aataattttt atcctaggga agcaaaggtg cagtggaaag tcgataacgc attgcaaagc     1800

ggtaacagtc aagaatctgt aactgaacaa gattctaaag attctaccta cagtctctcc     1860

tccacattga ccctgtcaaa agcagattat gagaagcaca aggtgtacgc atgtgaggta     1920

acacatcaag gactcagcag cccagttaca aaaagtttca atcgcgggga atgt           1974


<210>  17
<211>  1251
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence encoding the anti-CD20 CAR

<400>  17
atggagtgga gctgggtctt tctgttcttt ttaagcgtga ccaccggagt gcactcccag       60

atagtcctga gccaatcacc ggccatcttg tctgcctctc ctggcgaaaa ggtgacgatg      120

acttgcagag ccagtagctc tgtaagctat atacactggt tccagcaaaa accgggctct      180

tctccgaagc cgtggatata cgcaacttca aacctggcgt ctggggttcc tgtaaggttt      240

agcggcagcg gttcaggcac gagctacagc cttactatct cccgggttga ggctgaagat      300

gcagccacat actactgtca gcagtggact tcaaatccac ctacattcgg gggaggcacg      360

aagctggaga ttaaaggcgg cggcggcagc ggtggcggtg gctcaggtgg tggtggttct      420

caagtgcaat tgcagcagcc cggtgccgaa ctcgtgaaac caggagcaag cgtaaagatg      480

tcctgtaagg catcaggtta tacctttacc agctacaaca tgcactgggt gaaacaaacg      540

ccggggcggg gcctcgaatg gataggcgcg atatatcccg gaaatggcga taccagttac      600

aatcagaagt tcaaaggcaa agcgacactg acagctgata agtcttcaag caccgcctat      660

atgcaacttt ctagcctgac cagcgaagac tccgccgttt attactgtgc tcggtccaca      720

tactacggag gcgattggta ctttaatgtg tggggtgcgg gcaccactgt cactgtatca      780

gcgcccagaa agatagaggt gatgtaccct cccccctact tggacaacga aaagtctaat      840

ggcactatca ttcacgtaaa gggcaaacac ctttgtccaa gtcctttgtt cccaggccca      900

tctaagccgt tctgggtact cgtggttgtg gggggcgtgc tcgcttgtta ctcactgctg      960

gtgacggtgg cctttattat tttctgggtt aaacggggca gaaagaaact cctgtatata     1020

ttcaaacaac catttatgag accagtacaa actactcaag aggaagatgg ctgtagctgc     1080

cgatttccag aagaagaaga aggaggatgt gaactgaggg taaaatttag caggtctgca     1140

gataaagggg agaggagacg cgggaagggc catgatggac tgtatcaggg actttccaca     1200

gccaccaagg acacctatga cgctctccac atgcaggccc tgccccctcg c              1251


<210>  18
<211>  417
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-CD20 CAR precursor polypeptide

<400>  18

Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      


Val His Ser Gln Ile Val Leu Ser Gln Ser Pro Ala Ile Leu Ser Ala 
            20                  25                  30          


Ser Pro Gly Glu Lys Val Thr Met Thr Cys Arg Ala Ser Ser Ser Val 
        35                  40                  45              


Ser Tyr Ile His Trp Phe Gln Gln Lys Pro Gly Ser Ser Pro Lys Pro 
    50                  55                  60                  


Trp Ile Tyr Ala Thr Ser Asn Leu Ala Ser Gly Val Pro Val Arg Phe 
65                  70                  75                  80  


Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Arg Val 
                85                  90                  95      


Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Thr Ser Asn 
            100                 105                 110         


Pro Pro Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Gly Gly Gly 
        115                 120                 125             


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val Gln Leu 
    130                 135                 140                 


Gln Gln Pro Gly Ala Glu Leu Val Lys Pro Gly Ala Ser Val Lys Met 
145                 150                 155                 160 


Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr Asn Met His Trp 
                165                 170                 175     


Val Lys Gln Thr Pro Gly Arg Gly Leu Glu Trp Ile Gly Ala Ile Tyr 
            180                 185                 190         


Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe Lys Gly Lys Ala 
        195                 200                 205             


Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr Met Gln Leu Ser 
    210                 215                 220                 


Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys Ala Arg Ser Thr 
225                 230                 235                 240 


Tyr Tyr Gly Gly Asp Trp Tyr Phe Asn Val Trp Gly Ala Gly Thr Thr 
                245                 250                 255     


Val Thr Val Ser Ala Pro Arg Lys Ile Glu Val Met Tyr Pro Pro Pro 
            260                 265                 270         


Tyr Leu Asp Asn Glu Lys Ser Asn Gly Thr Ile Ile His Val Lys Gly 
        275                 280                 285             


Lys His Leu Cys Pro Ser Pro Leu Phe Pro Gly Pro Ser Lys Pro Phe 
    290                 295                 300                 


Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu 
305                 310                 315                 320 


Val Thr Val Ala Phe Ile Ile Phe Trp Val Lys Arg Gly Arg Lys Lys 
                325                 330                 335     


Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr 
            340                 345                 350         


Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly 
        355                 360                 365             


Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Lys Gly Glu 
    370                 375                 380                 


Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
385                 390                 395                 400 


Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro 
                405                 410                 415     


Arg 
    


<210>  19
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR GS linker

<400>  19

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  20
<211>  195
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Jet promoter

<400>  20
gaattcgggc ggagttaggg cggagccaat cagcgtgcgc cgttccgaaa gttgcctttt       60

atggctgggc ggagaatggg cggtgaacgc cgatgattat ataaggacgc gccgggtgtg      120

gcacagctag ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt ggatccctgt      180

gatcgtcact tgaca                                                       195


<210>  21
<211>  660
<212>  DNA
<213>  Homo sapiens

<400>  21
ggcaccatat tcattttgca ggtgaaattc ctgagatgta aggagctgct gtgacttgct       60

caaggcctta tatcgagtaa acggtagtgc tggggcttag acgcaggtgt tctgatttat      120

agttcaaaac ctctatcaat gagagagcaa tctcctggta atgtgataga tttcccaact      180

taatgccaac ataccataaa cctcccattc tgctaatgcc cagcctaagt tggggagacc      240

actccagatt ccaagatgta cagtttgctt tgctgggcct ttttcccatg cctgccttta      300

ctctgccaga gttatattgc tggggttttg aagaagatcc tattaaataa aagaataagc      360

agtattatta agtagccctg catttcaggt ttccttgagt ggcaggccag gcctggccgt      420

gaacgttcac tgaaatcatg gcctcttggc caagattgat agcttgtgcc tgtccctgag      480

tcccagtcca tcacgagcag ctggtttcta agatgctatt tcccgtataa agcatgagac      540

cgtgacttgc cagccccaca gagccccgcc cttgtccatc actggcatct ggactccagc      600

ctgggttggg gcaaagaggg aaatgagatc atgtcctaac cctgatcctc ttgtcccaca      660


<210>  22
<211>  650
<212>  DNA
<213>  Homo sapiens

<400>  22
gatatccaga accctgaccc tgccgtgtac cagctgagag actctaaatc cagtgacaag       60

tctgtctgcc tattcaccga ttttgattct caaacaaatg tgtcacaaag taaggattct      120

gatgtgtata tcacagacaa aactgtgcta gacatgaggt ctatggactt caagagcaac      180

agtgctgtgg cctggagcaa caaatctgac tttgcatgtg caaacgcctt caacaacagc      240

attattccag aggacacctt cttccccagc ccaggtaagg gcagctttgg tgccttcgca      300

ggctgtttcc ttgcttcagg aatggccagg ttctgcccag agctctggtc aatgatgtct      360

aaaactcctc tgattggtgg tctcggcctt atccattgcc accaaaaccc tctttttact      420

aagaaacagt gagccttgtt ctggcagtcc agagaatgac acgggaaaaa agcagatgaa      480

gagaaggtgg caggagaggg cacgtggccc agcctcagtc tctccaactg agttcctgcc      540

tgcctgcctt tgctcagact gtttgcccct tactgctctt ctaggcctca ttctaagccc      600

cttctccaag ttgcctctcc ttatttctcc ctgtctgcca aaaaatcttt                 650


<210>  23
<211>  19
<212>  DNA
<213>  Homo sapiens

<400>  23
cagggttctg gatatctgt                                                    19


<210>  24
<211>  171
<212>  DNA
<213>  Homo sapiens

<400>  24
atcacgagca gctggtttct aagatgctat ttcccgtata aagcatgaga ccgtgacttg       60

ccagccccac agagccccgc ccttgtccat cactggcatc tggactccag cctgggttgg      120

ggcaaagagg gaaatgagat catgtcctaa ccctgatcct cttgtcccac a               171


<210>  25
<211>  161
<212>  DNA
<213>  Homo sapiens

<400>  25
gatatccaga accctgaccc tgccgtgtac cagctgagag actctaaatc cagtgacaag       60

tctgtctgcc tattcaccga ttttgattct caaacaaatg tgtcacaaag taaggattct      120

gatgtgtata tcacagacaa aactgtgcta gacatgaggt c                          161


<210>  26
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  phosphorothioate linkage

<220>
<221>  misc_feature
<222>  (3)..(4)
<223>  phosphorothioate linkage

<220>
<221>  modified_base
<222>  (3)..(3)
<223>  cm

<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  phosphorothioate linkage

<220>
<221>  modified_base
<222>  (4)..(4)
<223>  2'-O-methyladenosine

<220>
<221>  modified_base
<222>  (5)..(5)
<223>  cm

<400>  26
atcacgagca gctggtttct                                                   20


<210>  27
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer

<400>  27
gacctcatgt ctagcacagt tttg                                              24


<210>  28
<211>  645
<212>  DNA
<213>  Homo sapiens

<400>  28
tgtaaggagc tgctgtgact tgctcaaggc cttatatcga gtaaacggta gtgctggggc       60

ttagacgcag gtgttctgat ttatagttca aaacctctat caatgagaga gcaatctcct      120

ggtaatgtga tagatttccc aacttaatgc caacatacca taaacctccc attctgctaa      180

tgcccagcct aagttgggga gaccactcca gattccaaga tgtacagttt gctttgctgg      240

gcctttttcc catgcctgcc tttactctgc cagagttata ttgctggggt tttgaagaag      300

atcctattaa ataaaagaat aagcagtatt attaagtagc cctgcatttc aggtttcctt      360

gagtggcagg ccaggcctgg ccgtgaacgt tcactgaaat catggcctct tggccaagat      420

tgatagcttg tgcctgtccc tgagtcccag tccatcacga gcagctggtt tctaagatgc      480

tatttcccgt ataaagcatg agaccgtgac ttgccagccc cacagagccc cgcccttgtc      540

catcactggc atctggactc cagcctgggt tggggcaaag agggaaatga gatcatgtcc      600

taaccctgat cctcttgtcc cacagatatc cagaaccctg accct                      645


<210>  29
<211>  600
<212>  DNA
<213>  Homo sapiens

<400>  29
gtaccagctg agagactcac tatccagtga caagtctgtc tgcctattca ccgattttga       60

ttctcaaaca aatgtgtcac aaagtaagga ttctgatgtg tatatcacag acaaaactgt      120

gctagacatg aggtctatgg acttcaagag caacagtgct gtggcctgga gcaacaaatc      180

tgactttgca tgtgcaaacg ccttcaacaa cagcattatt ccagaggaca ccttcttccc      240

cagcccaggt aagggcagct ttggtgcctt cgcaggctgt ttccttgctt caggaatggc      300

caggttctgc ccagagctct ggtcaatgat gtctaaaact cctctgattg gtggtctcgg      360

ccttatccat tgccaccaaa accctctttt tactaagaaa cagtgagcct tgttctggca      420

gtccagagaa tgacacggga aaaaagcaga tgaagagaag gtggcaggag agggcacgtg      480

gcccagcctc agtctctcca actgagttcc tgcctgcctg cctttgctca gactgtttgc      540

cccttactgc tcttctaggc ctcattctaa gccccttctc caagttgcct ctccttattt      600


<210>  30
<211>  21
<212>  DNA
<213>  Homo sapiens

<400>  30
gagtctctca gctggtacac g                                                 21


<210>  31
<211>  192
<212>  DNA
<213>  Homo sapiens

<400>  31
atcacgagca gctggtttct aagatgctat ttcccgtata aagcatgaga ccgtgacttg       60

ccagccccac agagccccgc ccttgtccat cactggcatc tggactccag cctgggttgg      120

ggcaaagagg gaaatgagat catgtcctaa ccctgatcct cttgtcccac agatatccag      180

aaccctgacc ct                                                          192


<210>  32
<211>  159
<212>  DNA
<213>  Homo sapiens

<400>  32
gtaccagctg agagactcac tatccagtga caagtctgtc tgcctattca ccgattttga       60

ttctcaaaca aatgtgtcac aaagtaagga ttctgatgtg tatatcacag acaaaactgt      120

gctagacatg aggtctatgg acttcaagag caacagtgc                             159


<210>  33
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for generating donor for insertion into Cas12a 
       site


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' phosphate

<400>  33
atcacgagca gctggtttct                                                   20


<210>  34
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial Reverse primer for generating donor for insertion into
       Cas12a site


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  phosphorothioate linkage

<220>
<221>  modified_base
<222>  (1)..(1)
<223>  gm

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  phosphorothioate linkage

<220>
<221>  modified_base
<222>  (2)..(2)
<223>  cm

<220>
<221>  misc_feature
<222>  (3)..(4)
<223>  phosphorothioate linkage

<220>
<221>  modified_base
<222>  (3)..(3)
<223>  2'-O-methyladenosine

<400>  34
gcactgttgc tcttgaagtc c                                                 21


<210>  35
<211>  48
<212>  PRT
<213>  Homo sapiens

<400>  35

Ala Lys Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro 
1               5                   10                  15      


Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro 
            20                  25                  30          


Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Pro Arg 
        35                  40                  45              


<210>  36
<211>  88
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8 hinge + CD28 hinge (long hinge)

<400>  36

Ala Lys Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro 
1               5                   10                  15      


Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro 
            20                  25                  30          


Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Pro Arg 
        35                  40                  45              


Lys Ile Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser 
    50                  55                  60                  


Asn Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro 
65                  70                  75                  80  


Leu Phe Pro Gly Pro Ser Lys Pro 
                85              


<210>  37
<211>  23
<212>  PRT
<213>  Homo sapiens

<400>  37

Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
1               5                   10                  15      


Ser Leu Val Ile Thr Leu Tyr 
            20              


<210>  38
<211>  27
<212>  PRT
<213>  Homo sapiens

<400>  38

Ile Ile Ser Phe Phe Leu Ala Leu Thr Ser Thr Ala Leu Leu Phe Leu 
1               5                   10                  15      


Leu Phe Phe Leu Thr Leu Arg Phe Ser Val Val 
            20                  25          


<210>  39
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  39

Leu Cys Tyr Leu Leu Asp Gly Ile Leu Phe Ile Tyr Gly Val Ile Leu 
1               5                   10                  15      


Thr Ala Leu Phe Leu 
            20      


<210>  40
<211>  41
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 co-stimulatory sequence

<400>  40

Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40      


<210>  41
<211>  42
<212>  PRT
<213>  Homo sapiens

<400>  41

Ala Leu Tyr Leu Leu Arg Arg Asp Gln Arg Leu Pro Pro Asp Ala His 
1               5                   10                  15      


Lys Pro Pro Gly Gly Gly Ser Phe Arg Thr Pro Ile Gln Glu Glu Gln 
            20                  25                  30          


Ala Asp Ala His Ser Thr Leu Ala Lys Ile 
        35                  40          


<210>  42
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD3zeta, including ITAMs 1, 2, and 3

<400>  42

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  43
<211>  38
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD3zeta ITAM 1

<400>  43

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg 
        35              


<210>  44
<211>  47
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD3zeta ITAM 2

<400>  44

Arg Val Lys Phe Ser Arg Ser Ala Asp Arg Gly Arg Asp Pro Glu Met 
1               5                   10                  15      


Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu 
            20                  25                  30          


Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met 
        35                  40                  45          


<210>  45
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD3zeta ITAM 3

<400>  45

Arg Val Lys Phe Ser Arg Ser Ala Asp Lys Gly Glu Arg Arg Arg Gly 
1               5                   10                  15      


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
            20                  25                  30          


Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
        35                  40                  45  


<210>  46
<211>  153
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DAR V1 intracellular domain

<400>  46

Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser 
        35                  40                  45              


Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu 
    50                  55                  60                  


Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg 
65                  70                  75                  80  


Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln 
                85                  90                  95      


Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr 
            100                 105                 110         


Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp 
        115                 120                 125             


Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala 
    130                 135                 140                 


Leu His Met Gln Ala Leu Pro Pro Arg 
145                 150             


<210>  47
<211>  154
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DAR V2a intracellular domain

<400>  47

Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg 
        35                  40                  45              


Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
    50                  55                  60                  


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
65                  70                  75                  80  


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
                85                  90                  95      


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
            100                 105                 110         


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
        115                 120                 125             


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
    130                 135                 140                 


Ala Leu His Met Gln Ala Leu Pro Pro Arg 
145                 150                 


<210>  48
<211>  153
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DAR V2b and CAR (28Z) intracellular domain

<400>  48

Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser 
        35                  40                  45              


Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu 
    50                  55                  60                  


Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg 
65                  70                  75                  80  


Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln 
                85                  90                  95      


Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr 
            100                 105                 110         


Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp 
        115                 120                 125             


Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala 
    130                 135                 140                 


Leu His Met Gln Ala Leu Pro Pro Arg 
145                 150             


<210>  49
<211>  195
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DAR V2c intracellular domain

<400>  49

Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Ser Lys Arg Ser Arg 
        35                  40                  45              


Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro 
    50                  55                  60                  


Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala 
65                  70                  75                  80  


Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr 
                85                  90                  95      


Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg 
            100                 105                 110         


Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met 
        115                 120                 125             


Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu 
    130                 135                 140                 


Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys 
145                 150                 155                 160 


Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu 
                165                 170                 175     


Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu 
            180                 185                 190         


Pro Pro Arg 
        195 


<210>  50
<211>  87
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DAR V3 and V4 intracellular domain

<400>  50

Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg 
        35                  40                  45              


Ser Ala Asp Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu 
    50                  55                  60                  


Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His 
65                  70                  75                  80  


Met Gln Ala Leu Pro Pro Arg 
                85          


<210>  51
<211>  120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DAR V3 intracellular domain

<400>  51

Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Ser Lys Arg Ser Arg 
        35                  40                  45              


Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro 
    50                  55                  60                  


Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala 
65                  70                  75                  80  


Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser Ala Asp Lys Gly Glu Arg 
                85                  90                  95      


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
            100                 105                 110         


Thr Lys Asp Thr Tyr Asp Ala Leu 
        115                 120 


<210>  52
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  52

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro 
            20      


<210>  53
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P2A self-cleaving sequence

<400>  53

Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
1               5                   10                  15      


Glu Glu Asn Pro Gly Pro 
            20          


<210>  54
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  E2A self-cleaving sequence

<400>  54

Gly Ser Gly Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp 
1               5                   10                  15      


Val Glu Ser Asn Pro Gly Pro 
            20              


<210>  55
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  F2A self-cleaving sequence

<400>  55

Gly Ser Gly Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala 
1               5                   10                  15      


Gly Asp Val Glu Ser Asn Pro Gly Pro 
            20                  25  


<210>  56
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge sequence

<400>  56

Pro Ala Pro Glu Leu Leu Gly Gly Pro 
1               5                   


<210>  57
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CH2 region

<400>  57

Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
1               5                   10          


<210>  58
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge sequence

<400>  58

Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
1               5                   10                  15      


Pro Glu Leu Leu Gly Gly Pro 
            20              


<210>  59
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge sequence

<400>  59

Glu Pro Lys Ser Cys Asp Lys Thr His Thr 
1               5                   10  


<210>  60
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge sequence


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa = Pro, Arg, or Ser

<400>  60

Cys Pro Xaa Cys 
1               


<210>  61
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge sequence

<400>  61

Cys Pro Pro Cys 
1               


<210>  62
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge sequence

<400>  62

Ser Pro Pro Cys 
1               


