                         SEQUENCE LISTING



<110>  Northwestern University

       Batlle, Daniel

       Jan Wysocki

       

 

<120>  ACTIVE LOW MOLECULAR WEIGHT VARIANTS OF ANGIOTENSIN CONVERTING 

       ENZYME 2 (ACE2)



<130>  702581.01725



<160>  9     



<170>  PatentIn version 3.5



<210>  1

<211>  805

<212>  PRT

<213>  Homo sapiens



<400>  1



Met Ser Ser Ser Ser Trp Leu Leu Leu Ser Leu Val Ala Val Thr Ala 

1               5                   10                  15      





Ala Gln Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe 

            20                  25                  30          





Asn His Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp 

        35                  40                  45              





Asn Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn 

    50                  55                  60                  





Ala Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala 

65                  70                  75                  80  





Gln Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln 

                85                  90                  95      





Leu Gln Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp Lys 

            100                 105                 110         





Ser Lys Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser 

        115                 120                 125             





Thr Gly Lys Val Cys Asn Pro Asp Asn Pro Gln Glu Cys Leu Leu Leu 

    130                 135                 140                 





Glu Pro Gly Leu Asn Glu Ile Met Ala Asn Ser Leu Asp Tyr Asn Glu 

145                 150                 155                 160 





Arg Leu Trp Ala Trp Glu Ser Trp Arg Ser Glu Val Gly Lys Gln Leu 

                165                 170                 175     





Arg Pro Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg 

            180                 185                 190         





Ala Asn His Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu 

        195                 200                 205             





Val Asn Gly Val Asp Gly Tyr Asp Tyr Ser Arg Gly Gln Leu Ile Glu 

    210                 215                 220                 





Asp Val Glu His Thr Phe Glu Glu Ile Lys Pro Leu Tyr Glu His Leu 

225                 230                 235                 240 





His Ala Tyr Val Arg Ala Lys Leu Met Asn Ala Tyr Pro Ser Tyr Ile 

                245                 250                 255     





Ser Pro Ile Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly 

            260                 265                 270         





Arg Phe Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Gly Gln Lys 

        275                 280                 285             





Pro Asn Ile Asp Val Thr Asp Ala Met Val Asp Gln Ala Trp Asp Ala 

    290                 295                 300                 





Gln Arg Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu 

305                 310                 315                 320 





Pro Asn Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Asp Pro 

                325                 330                 335     





Gly Asn Val Gln Lys Ala Val Cys His Pro Thr Ala Trp Asp Leu Gly 

            340                 345                 350         





Lys Gly Asp Phe Arg Ile Leu Met Cys Thr Lys Val Thr Met Asp Asp 

        355                 360                 365             





Phe Leu Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala 

    370                 375                 380                 





Tyr Ala Ala Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe 

385                 390                 395                 400 





His Glu Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys 

                405                 410                 415     





His Leu Lys Ser Ile Gly Leu Leu Ser Pro Asp Phe Gln Glu Asp Asn 

            420                 425                 430         





Glu Thr Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly 

        435                 440                 445             





Thr Leu Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe 

    450                 455                 460                 





Lys Gly Glu Ile Pro Lys Asp Gln Trp Met Lys Lys Trp Trp Glu Met 

465                 470                 475                 480 





Lys Arg Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr 

                485                 490                 495     





Tyr Cys Asp Pro Ala Ser Leu Phe His Val Ser Asn Asp Tyr Ser Phe 

            500                 505                 510         





Ile Arg Tyr Tyr Thr Arg Thr Leu Tyr Gln Phe Gln Phe Gln Glu Ala 

        515                 520                 525             





Leu Cys Gln Ala Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile 

    530                 535                 540                 





Ser Asn Ser Thr Glu Ala Gly Gln Lys Leu Phe Asn Met Leu Arg Leu 

545                 550                 555                 560 





Gly Lys Ser Glu Pro Trp Thr Leu Ala Leu Glu Asn Val Val Gly Ala 

                565                 570                 575     





Lys Asn Met Asn Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe 

            580                 585                 590         





Thr Trp Leu Lys Asp Gln Asn Lys Asn Ser Phe Val Gly Trp Ser Thr 

        595                 600                 605             





Asp Trp Ser Pro Tyr Ala Asp Gln Ser Ile Lys Val Arg Ile Ser Leu 

    610                 615                 620                 





Lys Ser Ala Leu Gly Asp Lys Ala Tyr Glu Trp Asn Asp Asn Glu Met 

625                 630                 635                 640 





Tyr Leu Phe Arg Ser Ser Val Ala Tyr Ala Met Arg Gln Tyr Phe Leu 

                645                 650                 655     





Lys Val Lys Asn Gln Met Ile Leu Phe Gly Glu Glu Asp Val Arg Val 

            660                 665                 670         





Ala Asn Leu Lys Pro Arg Ile Ser Phe Asn Phe Phe Val Thr Ala Pro 

        675                 680                 685             





Lys Asn Val Ser Asp Ile Ile Pro Arg Thr Glu Val Glu Lys Ala Ile 

    690                 695                 700                 





Arg Met Ser Arg Ser Arg Ile Asn Asp Ala Phe Arg Leu Asn Asp Asn 

705                 710                 715                 720 





Ser Leu Glu Phe Leu Gly Ile Gln Pro Thr Leu Gly Pro Pro Asn Gln 

                725                 730                 735     





Pro Pro Val Ser Ile Trp Leu Ile Val Phe Gly Val Val Met Gly Val 

            740                 745                 750         





Ile Val Val Gly Ile Val Ile Leu Ile Phe Thr Gly Ile Arg Asp Arg 

        755                 760                 765             





Lys Lys Lys Asn Lys Ala Arg Ser Gly Glu Asn Pro Tyr Ala Ser Ile 

    770                 775                 780                 





Asp Ile Ser Lys Gly Glu Asn Asn Pro Gly Phe Gln Asn Thr Asp Asp 

785                 790                 795                 800 





Val Gln Thr Ser Phe 

                805 





<210>  2

<211>  805

<212>  PRT

<213>  Mus musculus



<400>  2



Met Ser Ser Ser Ser Trp Leu Leu Leu Ser Leu Val Ala Val Thr Thr 

1               5                   10                  15      





Ala Gln Ser Leu Thr Glu Glu Asn Ala Lys Thr Phe Leu Asn Asn Phe 

            20                  25                  30          





Asn Gln Glu Ala Glu Asp Leu Ser Tyr Gln Ser Ser Leu Ala Ser Trp 

        35                  40                  45              





Asn Tyr Asn Thr Asn Ile Thr Glu Glu Asn Ala Gln Lys Met Ser Glu 

    50                  55                  60                  





Ala Ala Ala Lys Trp Ser Ala Phe Tyr Glu Glu Gln Ser Lys Thr Ala 

65                  70                  75                  80  





Gln Ser Phe Ser Leu Gln Glu Ile Gln Thr Pro Ile Ile Lys Arg Gln 

                85                  90                  95      





Leu Gln Ala Leu Gln Gln Ser Gly Ser Ser Ala Leu Ser Ala Asp Lys 

            100                 105                 110         





Asn Lys Gln Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser 

        115                 120                 125             





Thr Gly Lys Val Cys Asn Pro Lys Asn Pro Gln Glu Cys Leu Leu Leu 

    130                 135                 140                 





Glu Pro Gly Leu Asp Glu Ile Met Ala Thr Ser Thr Asp Tyr Asn Ser 

145                 150                 155                 160 





Arg Leu Trp Ala Trp Glu Gly Trp Arg Ala Glu Val Gly Lys Gln Leu 

                165                 170                 175     





Arg Pro Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg 

            180                 185                 190         





Ala Asn Asn Tyr Asn Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu 

        195                 200                 205             





Ala Glu Gly Ala Asp Gly Tyr Asn Tyr Asn Arg Asn Gln Leu Ile Glu 

    210                 215                 220                 





Asp Val Glu Arg Thr Phe Ala Glu Ile Lys Pro Leu Tyr Glu His Leu 

225                 230                 235                 240 





His Ala Tyr Val Arg Arg Lys Leu Met Asp Thr Tyr Pro Ser Tyr Ile 

                245                 250                 255     





Ser Pro Thr Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly 

            260                 265                 270         





Arg Phe Trp Thr Asn Leu Tyr Pro Leu Thr Val Pro Phe Ala Gln Lys 

        275                 280                 285             





Pro Asn Ile Asp Val Thr Asp Ala Met Met Asn Gln Gly Trp Asp Ala 

    290                 295                 300                 





Glu Arg Ile Phe Gln Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu 

305                 310                 315                 320 





Pro His Met Thr Gln Gly Phe Trp Ala Asn Ser Met Leu Thr Glu Pro 

                325                 330                 335     





Ala Asp Gly Arg Lys Val Val Cys His Pro Thr Ala Trp Asp Leu Gly 

            340                 345                 350         





His Gly Asp Phe Arg Ile Lys Met Cys Thr Lys Val Thr Met Asp Asn 

        355                 360                 365             





Phe Leu Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala 

    370                 375                 380                 





Tyr Ala Arg Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe 

385                 390                 395                 400 





His Glu Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys 

                405                 410                 415     





His Leu Lys Ser Ile Gly Leu Leu Pro Ser Asp Phe Gln Glu Asp Ser 

            420                 425                 430         





Glu Thr Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly 

        435                 440                 445             





Thr Leu Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe 

    450                 455                 460                 





Arg Gly Glu Ile Pro Lys Glu Gln Trp Met Lys Lys Trp Trp Glu Met 

465                 470                 475                 480 





Lys Arg Glu Ile Val Gly Val Val Glu Pro Leu Pro His Asp Glu Thr 

                485                 490                 495     





Tyr Cys Asp Pro Ala Ser Leu Phe His Val Ser Asn Asp Tyr Ser Phe 

            500                 505                 510         





Ile Arg Tyr Tyr Thr Arg Thr Ile Tyr Gln Phe Gln Phe Gln Glu Ala 

        515                 520                 525             





Leu Cys Gln Ala Ala Lys Tyr Asn Gly Ser Leu His Lys Cys Asp Ile 

    530                 535                 540                 





Ser Asn Ser Thr Glu Ala Gly Gln Lys Leu Leu Lys Met Leu Ser Leu 

545                 550                 555                 560 





Gly Asn Ser Glu Pro Trp Thr Lys Ala Leu Glu Asn Val Val Gly Ala 

                565                 570                 575     





Arg Asn Met Asp Val Lys Pro Leu Leu Asn Tyr Phe Gln Pro Leu Phe 

            580                 585                 590         





Asp Trp Leu Lys Glu Gln Asn Arg Asn Ser Phe Val Gly Trp Asn Thr 

        595                 600                 605             





Glu Trp Ser Pro Tyr Ala Asp Gln Ser Ile Lys Val Arg Ile Ser Leu 

    610                 615                 620                 





Lys Ser Ala Leu Gly Ala Asn Ala Tyr Glu Trp Thr Asn Asn Glu Met 

625                 630                 635                 640 





Phe Leu Phe Arg Ser Ser Val Ala Tyr Ala Met Arg Lys Tyr Phe Ser 

                645                 650                 655     





Ile Ile Lys Asn Gln Thr Val Pro Phe Leu Glu Glu Asp Val Arg Val 

            660                 665                 670         





Ser Asp Leu Lys Pro Arg Val Ser Phe Tyr Phe Phe Val Thr Ser Pro 

        675                 680                 685             





Gln Asn Val Ser Asp Val Ile Pro Arg Ser Glu Val Glu Asp Ala Ile 

    690                 695                 700                 





Arg Met Ser Arg Gly Arg Ile Asn Asp Val Phe Gly Leu Asn Asp Asn 

705                 710                 715                 720 





Ser Leu Glu Phe Leu Gly Ile His Pro Thr Leu Glu Pro Pro Tyr Gln 

                725                 730                 735     





Pro Pro Val Thr Ile Trp Leu Ile Ile Phe Gly Val Val Met Ala Leu 

            740                 745                 750         





Val Val Val Gly Ile Ile Ile Leu Ile Val Thr Gly Ile Lys Gly Arg 

        755                 760                 765             





Lys Lys Lys Asn Glu Thr Lys Arg Glu Glu Asn Pro Tyr Asp Ser Met 

    770                 775                 780                 





Asp Ile Gly Lys Gly Glu Ser Asn Ala Gly Phe Gln Asn Ser Asp Asp 

785                 790                 795                 800 





Ala Gln Thr Ser Phe 

                805 





<210>  3

<211>  619

<212>  PRT

<213>  Homo sapiens



<400>  3



Met Ser Ser Ser Ser Trp Leu Leu Leu Ser Leu Val Ala Val Thr Ala 

1               5                   10                  15      





Ala Gln Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe 

            20                  25                  30          





Asn His Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp 

        35                  40                  45              





Asn Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn 

    50                  55                  60                  





Ala Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala 

65                  70                  75                  80  





Gln Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln 

                85                  90                  95      





Leu Gln Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp Lys 

            100                 105                 110         





Ser Lys Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser 

        115                 120                 125             





Thr Gly Lys Val Cys Asn Pro Asp Asn Pro Gln Glu Cys Leu Leu Leu 

    130                 135                 140                 





Glu Pro Gly Leu Asn Glu Ile Met Ala Asn Ser Leu Asp Tyr Asn Glu 

145                 150                 155                 160 





Arg Leu Trp Ala Trp Glu Ser Trp Arg Ser Glu Val Gly Lys Gln Leu 

                165                 170                 175     





Arg Pro Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg 

            180                 185                 190         





Ala Asn His Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu 

        195                 200                 205             





Val Asn Gly Val Asp Gly Tyr Asp Tyr Ser Arg Gly Gln Leu Ile Glu 

    210                 215                 220                 





Asp Val Glu His Thr Phe Glu Glu Ile Lys Pro Leu Tyr Glu His Leu 

225                 230                 235                 240 





His Ala Tyr Val Arg Ala Lys Leu Met Asn Ala Tyr Pro Ser Tyr Ile 

                245                 250                 255     





Ser Pro Ile Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly 

            260                 265                 270         





Arg Phe Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Gly Gln Lys 

        275                 280                 285             





Pro Asn Ile Asp Val Thr Asp Ala Met Val Asp Gln Ala Trp Asp Ala 

    290                 295                 300                 





Gln Arg Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu 

305                 310                 315                 320 





Pro Asn Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Asp Pro 

                325                 330                 335     





Gly Asn Val Gln Lys Ala Val Cys His Pro Thr Ala Trp Asp Leu Gly 

            340                 345                 350         





Lys Gly Asp Phe Arg Ile Leu Met Cys Thr Lys Val Thr Met Asp Asp 

        355                 360                 365             





Phe Leu Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala 

    370                 375                 380                 





Tyr Ala Ala Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe 

385                 390                 395                 400 





His Glu Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys 

                405                 410                 415     





His Leu Lys Ser Ile Gly Leu Leu Ser Pro Asp Phe Gln Glu Asp Asn 

            420                 425                 430         





Glu Thr Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly 

        435                 440                 445             





Thr Leu Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe 

    450                 455                 460                 





Lys Gly Glu Ile Pro Lys Asp Gln Trp Met Lys Lys Trp Trp Glu Met 

465                 470                 475                 480 





Lys Arg Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr 

                485                 490                 495     





Tyr Cys Asp Pro Ala Ser Leu Phe His Val Ser Asn Asp Tyr Ser Phe 

            500                 505                 510         





Ile Arg Tyr Tyr Thr Arg Thr Leu Tyr Gln Phe Gln Phe Gln Glu Ala 

        515                 520                 525             





Leu Cys Gln Ala Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile 

    530                 535                 540                 





Ser Asn Ser Thr Glu Ala Gly Gln Lys Leu Phe Asn Met Leu Arg Leu 

545                 550                 555                 560 





Gly Lys Ser Glu Pro Trp Thr Leu Ala Leu Glu Asn Val Val Gly Ala 

                565                 570                 575     





Lys Asn Met Asn Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe 

            580                 585                 590         





Thr Trp Leu Lys Asp Gln Asn Lys Asn Ser Phe Val Gly Trp Ser Thr 

        595                 600                 605             





Asp Trp Ser Pro Tyr Ala Asp Gln Ser Ile Lys 

    610                 615                 





<210>  4

<211>  605

<212>  PRT

<213>  Homo sapiens



<400>  4



Met Ser Ser Ser Ser Trp Leu Leu Leu Ser Leu Val Ala Val Thr Ala 

1               5                   10                  15      





Ala Gln Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe 

            20                  25                  30          





Asn His Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp 

        35                  40                  45              





Asn Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn 

    50                  55                  60                  





Ala Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala 

65                  70                  75                  80  





Gln Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln 

                85                  90                  95      





Leu Gln Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp Lys 

            100                 105                 110         





Ser Lys Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser 

        115                 120                 125             





Thr Gly Lys Val Cys Asn Pro Asp Asn Pro Gln Glu Cys Leu Leu Leu 

    130                 135                 140                 





Glu Pro Gly Leu Asn Glu Ile Met Ala Asn Ser Leu Asp Tyr Asn Glu 

145                 150                 155                 160 





Arg Leu Trp Ala Trp Glu Ser Trp Arg Ser Glu Val Gly Lys Gln Leu 

                165                 170                 175     





Arg Pro Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg 

            180                 185                 190         





Ala Asn His Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu 

        195                 200                 205             





Val Asn Gly Val Asp Gly Tyr Asp Tyr Ser Arg Gly Gln Leu Ile Glu 

    210                 215                 220                 





Asp Val Glu His Thr Phe Glu Glu Ile Lys Pro Leu Tyr Glu His Leu 

225                 230                 235                 240 





His Ala Tyr Val Arg Ala Lys Leu Met Asn Ala Tyr Pro Ser Tyr Ile 

                245                 250                 255     





Ser Pro Ile Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly 

            260                 265                 270         





Arg Phe Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Gly Gln Lys 

        275                 280                 285             





Pro Asn Ile Asp Val Thr Asp Ala Met Val Asp Gln Ala Trp Asp Ala 

    290                 295                 300                 





Gln Arg Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu 

305                 310                 315                 320 





Pro Asn Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Asp Pro 

                325                 330                 335     





Gly Asn Val Gln Lys Ala Val Cys His Pro Thr Ala Trp Asp Leu Gly 

            340                 345                 350         





Lys Gly Asp Phe Arg Ile Leu Met Cys Thr Lys Val Thr Met Asp Asp 

        355                 360                 365             





Phe Leu Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala 

    370                 375                 380                 





Tyr Ala Ala Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe 

385                 390                 395                 400 





His Glu Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys 

                405                 410                 415     





His Leu Lys Ser Ile Gly Leu Leu Ser Pro Asp Phe Gln Glu Asp Asn 

            420                 425                 430         





Glu Thr Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly 

        435                 440                 445             





Thr Leu Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe 

    450                 455                 460                 





Lys Gly Glu Ile Pro Lys Asp Gln Trp Met Lys Lys Trp Trp Glu Met 

465                 470                 475                 480 





Lys Arg Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr 

                485                 490                 495     





Tyr Cys Asp Pro Ala Ser Leu Phe His Val Ser Asn Asp Tyr Ser Phe 

            500                 505                 510         





Ile Arg Tyr Tyr Thr Arg Thr Leu Tyr Gln Phe Gln Phe Gln Glu Ala 

        515                 520                 525             





Leu Cys Gln Ala Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile 

    530                 535                 540                 





Ser Asn Ser Thr Glu Ala Gly Gln Lys Leu Phe Asn Met Leu Arg Leu 

545                 550                 555                 560 





Gly Lys Ser Glu Pro Trp Thr Leu Ala Leu Glu Asn Val Val Gly Ala 

                565                 570                 575     





Lys Asn Met Asn Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe 

            580                 585                 590         





Thr Trp Leu Lys Asp Gln Asn Lys Asn Ser Phe Val Gly 

        595                 600                 605 





<210>  5

<211>  46

<212>  PRT

<213>  Artificial



<220>

<223>  Variant of Streptococcal G-protein albumin binding domain III



<400>  5



Leu Ala Glu Ala Lys Val Leu Ala Asn Arg Glu Leu Asp Lys Tyr Gly 

1               5                   10                  15      





Val Ser Asp Phe Tyr Lys Arg Leu Ile Asn Lys Ala Lys Thr Val Glu 

            20                  25                  30          





Gly Val Glu Ala Leu Lys Leu His Ile Leu Ala Ala Leu Pro 

        35                  40                  45      





<210>  6

<211>  232

<212>  PRT

<213>  Homo sapiens



<400>  6



Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 

1               5                   10                  15      





Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 

            20                  25                  30          





Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 

        35                  40                  45              





Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 

    50                  55                  60                  





Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 

65                  70                  75                  80  





Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 

                85                  90                  95      





Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 

            100                 105                 110         





Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 

        115                 120                 125             





Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr 

    130                 135                 140                 





Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 

145                 150                 155                 160 





Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 

                165                 170                 175     





Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 

            180                 185                 190         





Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 

        195                 200                 205             





Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 

    210                 215                 220                 





Ser Leu Ser Leu Ser Pro Gly Lys 

225                 230         





<210>  7

<211>  107

<212>  PRT

<213>  Homo sapien



<400>  7



Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Ser Pro Pro Ser Arg Asp 

1               5                   10                  15      





Glu Leu Thr Lys Asn Gln Val Ser Leu Arg Cys His Val Lys Gly Phe 

            20                  25                  30          





Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu 

        35                  40                  45              





Asn Asn Tyr Lys Thr Thr Lys Pro Val Leu Asp Ser Asp Gly Ser Phe 

    50                  55                  60                  





Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly 

65                  70                  75                  80  





Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr 

                85                  90                  95      





Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 

            100                 105         





<210>  8

<211>  107

<212>  PRT

<213>  Artificial



<220>

<223>  Modified amino acid sequence of the monomeric, 

       disulfide-stabilized mCH3 fragment of human IgG



<400>  8



Gly Gln Cys Arg Glu Pro Gln Val Tyr Thr Ser Pro Pro Ser Arg Asp 

1               5                   10                  15      





Glu Leu Thr Lys Asn Gln Val Ser Leu Arg Cys His Val Lys Gly Phe 

            20                  25                  30          





Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu 

        35                  40                  45              





Asn Asn Tyr Lys Thr Thr Lys Pro Val Leu Asp Ser Asp Gly Ser Phe 

    50                  55                  60                  





Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly 

65                  70                  75                  80  





Asn Val Phe Ser Cys Ser Val Met His Glu Cys Leu His Asn His Tyr 

                85                  90                  95      





Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 

            100                 105         





<210>  9

<211>  205

<212>  PRT

<213>  Homo sapiens



<400>  9



Leu Glu Lys Cys Cys Ala Ala Ala Asp Pro His Glu Cys Tyr Ala Lys 

1               5                   10                  15      





Val Phe Asp Glu Phe Lys Pro Leu Val Glu Glu Pro Gln Asn Leu Ile 

            20                  25                  30          





Lys Gln Asn Cys Glu Leu Phe Glu Gln Leu Gly Glu Tyr Lys Phe Gln 

        35                  40                  45              





Asn Ala Leu Leu Val Arg Tyr Thr Lys Lys Val Pro Gln Val Ser Thr 

    50                  55                  60                  





Pro Thr Leu Val Glu Val Ser Arg Asn Leu Gly Lys Val Gly Ser Lys 

65                  70                  75                  80  





Cys Cys Lys His Pro Glu Ala Lys Arg Met Pro Cys Ala Glu Asp Cys 

                85                  90                  95      





Leu Ser Val Phe Leu Asn Gln Leu Cys Val Leu His Glu Lys Thr Pro 

            100                 105                 110         





Val Ser Asp Arg Val Thr Lys Cys Cys Thr Glu Ser Leu Val Asn Gly 

        115                 120                 125             





Arg Pro Cys Phe Ser Ala Leu Glu Val Asp Glu Thr Tyr Val Pro Lys 

    130                 135                 140                 





Glu Phe Asn Ala Glu Thr Phe Thr Phe His Ala Asp Ile Cys Thr Leu 

145                 150                 155                 160 





Ser Glu Lys Glu Arg Gln Ile Lys Lys Gln Thr Ala Leu Val Glu Leu 

                165                 170                 175     





Val Lys His Lys Pro Lys Ala Thr Lys Glu Gln Leu Lys Ala Val Met 

            180                 185                 190         





Asp Asp Phe Ala Ala Phe Val Glu Lys Cys Cys Lys Ala 

        195                 200                 205 





<210>  10

<211>  618

<212>  PRT

<213>  Homo sapiens



<400>  10



Met Ser Ser Ser Ser Trp Leu Leu Leu Ser Leu Val Ala Val Thr Ala 

1               5                   10                  15      





Ala Gln Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe 

            20                  25                  30          





Asn His Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp 

        35                  40                  45              





Asn Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn 

    50                  55                  60                  





Ala Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala 

65                  70                  75                  80  





Gln Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln 

                85                  90                  95      





Leu Gln Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp Lys 

            100                 105                 110         





Ser Lys Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser 

        115                 120                 125             





Thr Gly Lys Val Cys Asn Pro Asp Asn Pro Gln Glu Cys Leu Leu Leu 

    130                 135                 140                 





Glu Pro Gly Leu Asn Glu Ile Met Ala Asn Ser Leu Asp Tyr Asn Glu 

145                 150                 155                 160 





Arg Leu Trp Ala Trp Glu Ser Trp Arg Ser Glu Val Gly Lys Gln Leu 

                165                 170                 175     





Arg Pro Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg 

            180                 185                 190         





Ala Asn His Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu 

        195                 200                 205             





Val Asn Gly Val Asp Gly Tyr Asp Tyr Ser Arg Gly Gln Leu Ile Glu 

    210                 215                 220                 





Asp Val Glu His Thr Phe Glu Glu Ile Lys Pro Leu Tyr Glu His Leu 

225                 230                 235                 240 





His Ala Tyr Val Arg Ala Lys Leu Met Asn Ala Tyr Pro Ser Tyr Ile 

                245                 250                 255     





Ser Pro Ile Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly 

            260                 265                 270         





Arg Phe Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Gly Gln Lys 

        275                 280                 285             





Pro Asn Ile Asp Val Thr Asp Ala Met Val Asp Gln Ala Trp Asp Ala 

    290                 295                 300                 





Gln Arg Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu 

305                 310                 315                 320 





Pro Asn Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Asp Pro 

                325                 330                 335     





Gly Asn Val Gln Lys Ala Val Cys His Pro Thr Ala Trp Asp Leu Gly 

            340                 345                 350         





Lys Gly Asp Phe Arg Ile Leu Met Cys Thr Lys Val Thr Met Asp Asp 

        355                 360                 365             





Phe Leu Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala 

    370                 375                 380                 





Tyr Ala Ala Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe 

385                 390                 395                 400 





His Glu Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys 

                405                 410                 415     





His Leu Lys Ser Ile Gly Leu Leu Ser Pro Asp Phe Gln Glu Asp Asn 

            420                 425                 430         





Glu Thr Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly 

        435                 440                 445             





Thr Leu Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe 

    450                 455                 460                 





Lys Gly Glu Ile Pro Lys Asp Gln Trp Met Lys Lys Trp Trp Glu Met 

465                 470                 475                 480 





Lys Arg Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr 

                485                 490                 495     





Tyr Cys Asp Pro Ala Ser Leu Phe His Val Ser Asn Asp Tyr Ser Phe 

            500                 505                 510         





Ile Arg Tyr Tyr Thr Arg Thr Leu Tyr Gln Phe Gln Phe Gln Glu Ala 

        515                 520                 525             





Leu Cys Gln Ala Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile 

    530                 535                 540                 





Ser Asn Ser Thr Glu Ala Gly Gln Lys Leu Phe Asn Met Leu Arg Leu 

545                 550                 555                 560 





Gly Lys Ser Glu Pro Trp Thr Leu Ala Leu Glu Asn Val Val Gly Ala 

                565                 570                 575     





Lys Asn Met Asn Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe 

            580                 585                 590         





Thr Trp Leu Lys Asp Gln Asn Lys Asn Ser Phe Val Gly Trp Ser Thr 

        595                 600                 605             





Asp Trp Ser Pro Tyr Ala Asp Gln Ser Ile 

    610                 615         





<210>  11

<211>  542

<212>  PRT

<213>  Homo sapiens



<400>  11



Met Ser Ser Ser Ser Trp Leu Leu Leu Ser Leu Val Ala Val Thr Ala 

1               5                   10                  15      





Ala Gln Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe 

            20                  25                  30          





Asn His Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp 

        35                  40                  45              





Asn Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn 

    50                  55                  60                  





Ala Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala 

65                  70                  75                  80  





Gln Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln 

                85                  90                  95      





Leu Gln Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp Lys 

            100                 105                 110         





Ser Lys Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser 

        115                 120                 125             





Thr Gly Lys Val Cys Asn Pro Asp Asn Pro Gln Glu Cys Leu Leu Leu 

    130                 135                 140                 





Glu Pro Gly Leu Asn Glu Ile Met Ala Asn Ser Leu Asp Tyr Asn Glu 

145                 150                 155                 160 





Arg Leu Trp Ala Trp Glu Ser Trp Arg Ser Glu Val Gly Lys Gln Leu 

                165                 170                 175     





Arg Pro Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg 

            180                 185                 190         





Ala Asn His Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu 

        195                 200                 205             





Val Asn Gly Val Asp Gly Tyr Asp Tyr Ser Arg Gly Gln Leu Ile Glu 

    210                 215                 220                 





Asp Val Glu His Thr Phe Glu Glu Ile Lys Pro Leu Tyr Glu His Leu 

225                 230                 235                 240 





His Ala Tyr Val Arg Ala Lys Leu Met Asn Ala Tyr Pro Ser Tyr Ile 

                245                 250                 255     





Ser Pro Ile Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly 

            260                 265                 270         





Arg Phe Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Gly Gln Lys 

        275                 280                 285             





Pro Asn Ile Asp Val Thr Asp Ala Met Val Asp Gln Ala Trp Asp Ala 

    290                 295                 300                 





Gln Arg Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu 

305                 310                 315                 320 





Pro Asn Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Asp Pro 

                325                 330                 335     





Gly Asn Val Gln Lys Ala Val Cys His Pro Thr Ala Trp Asp Leu Gly 

            340                 345                 350         





Lys Gly Asp Phe Arg Ile Leu Met Cys Thr Lys Val Thr Met Asp Asp 

        355                 360                 365             





Phe Leu Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala 

    370                 375                 380                 





Tyr Ala Ala Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe 

385                 390                 395                 400 





His Glu Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys 

                405                 410                 415     





His Leu Lys Ser Ile Gly Leu Leu Ser Pro Asp Phe Gln Glu Asp Asn 

            420                 425                 430         





Glu Thr Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly 

        435                 440                 445             





Thr Leu Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe 

    450                 455                 460                 





Lys Gly Glu Ile Pro Lys Asp Gln Trp Met Lys Lys Trp Trp Glu Met 

465                 470                 475                 480 





Lys Arg Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr 

                485                 490                 495     





Tyr Cys Asp Pro Ala Ser Leu Phe His Val Ser Asn Asp Tyr Ser Phe 

            500                 505                 510         





Ile Arg Tyr Tyr Thr Arg Thr Leu Tyr Gln Phe Gln Phe Gln Glu Ala 

        515                 520                 525             





Leu Cys Gln Ala Ala Lys His Glu Gly Pro Leu His Lys Cys  

    530                 535                 540                 





<210>  12

<211>  522

<212>  PRT

<213>  Homo sapiens



<400>  12



Met Ser Ser Ser Ser Trp Leu Leu Leu Ser Leu Val Ala Val Thr Ala 

1               5                   10                  15      





Ala Gln Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe 

            20                  25                  30          





Asn His Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp 

        35                  40                  45              





Asn Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn 

    50                  55                  60                  





Ala Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala 

65                  70                  75                  80  





Gln Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln 

                85                  90                  95      





Leu Gln Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp Lys 

            100                 105                 110         





Ser Lys Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser 

        115                 120                 125             





Thr Gly Lys Val Cys Asn Pro Asp Asn Pro Gln Glu Cys Leu Leu Leu 

    130                 135                 140                 





Glu Pro Gly Leu Asn Glu Ile Met Ala Asn Ser Leu Asp Tyr Asn Glu 

145                 150                 155                 160 





Arg Leu Trp Ala Trp Glu Ser Trp Arg Ser Glu Val Gly Lys Gln Leu 

                165                 170                 175     





Arg Pro Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg 

            180                 185                 190         





Ala Asn His Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu 

        195                 200                 205             





Val Asn Gly Val Asp Gly Tyr Asp Tyr Ser Arg Gly Gln Leu Ile Glu 

    210                 215                 220                 





Asp Val Glu His Thr Phe Glu Glu Ile Lys Pro Leu Tyr Glu His Leu 

225                 230                 235                 240 





His Ala Tyr Val Arg Ala Lys Leu Met Asn Ala Tyr Pro Ser Tyr Ile 

                245                 250                 255     





Ser Pro Ile Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly 

            260                 265                 270         





Arg Phe Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Gly Gln Lys 

        275                 280                 285             





Pro Asn Ile Asp Val Thr Asp Ala Met Val Asp Gln Ala Trp Asp Ala 

    290                 295                 300                 





Gln Arg Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu 

305                 310                 315                 320 





Pro Asn Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Asp Pro 

                325                 330                 335     





Gly Asn Val Gln Lys Ala Val Cys His Pro Thr Ala Trp Asp Leu Gly 

            340                 345                 350         





Lys Gly Asp Phe Arg Ile Leu Met Cys Thr Lys Val Thr Met Asp Asp 

        355                 360                 365             





Phe Leu Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala 

    370                 375                 380                 





Tyr Ala Ala Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe 

385                 390                 395                 400 





His Glu Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys 

                405                 410                 415     





His Leu Lys Ser Ile Gly Leu Leu Ser Pro Asp Phe Gln Glu Asp Asn 

            420                 425                 430         





Glu Thr Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly 

        435                 440                 445             





Thr Leu Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe 

    450                 455                 460                 





Lys Gly Glu Ile Pro Lys Asp Gln Trp Met Lys Lys Trp Trp Glu Met 

465                 470                 475                 480 





Lys Arg Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr 

                485                 490                 495     





Tyr Cys Asp Pro Ala Ser Leu Phe His Val Ser Asn Asp Tyr Ser Phe 

            500                 505                 510         





Ile Arg Tyr Tyr Thr Arg Thr Leu Tyr Gln 

        515                 520                        



      

<210>  13

<211>  45

<212>  PRT

<213>  Artificial (Consensus Albumin Binding Domain (ABDCon)



<400>  13



Leu Lys Glu Ala Lys Glu Lys Ala Ile Glu Glu Leu Lys Lys Ala Gly

1               5                   10                  15





Ile Thr Ser Asp Tyr Tyr Phe Asp Leu Ile Asn Lys Ala Lys Thr Val

            20                  25                  30        





Glu Gly Val Asn Ala Leu Lys Asp Glu Ile Leu Lys Ala

        35                  40                  45





<210>  14

<211>  18

<212>  PRT

<213>  Artificial (Consensus Albumin Binding Domain (ABDCon)



<400>  14



Gly Gly Ser Ser Arg Ser Ser Ser Ser Gly Gly Gly Gly Ser Gly Gly

1               5                   10                  15





Gly Gly

               



<210>  15

<211>  12

<212>  PRT

<213>  Homo sapiens



<400>  15



Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro

1               5                   10





<210>  16

<211>  13

<212>  PRT

<213>  Artificial (Modified Hinge Region)


<400>  16



Lys Cys His Trp Glu Cys Arg Gly Cys Arg Leu Val Cys

1               5                   10



