			SEQUENCE LISTING

<110> CODEXIS, INC.
 
<120> HUMAN ALPHA-GALACTOSIDASE VARIANTS
 
<130> CX7-203WO2
 
<140> 
<141> 
 
<150> US 62/982,949
<151> 2020-02-28
 
<160> 1864
 
<210> 1
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> wild type human alpha-galactosidase A
 
<400> 1
ttggataacg ggttagcccg tacacctact atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcatggag 120
atggctgaac taatggtaag tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aactacgtac acagcaaggg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggagcat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catggaagtc aatcaaatct atcttggatt ggacttcttt caaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggaa tcaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
gcacccctat tcatgtctaa tgatctacgt cacatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agacaaggtg acaactttga agtgtgggaa agaccattgt ctggacttgc gtgggctgtt 960
gctatgatca accgtcaaga gatcggaggg ccaagatctt acactatcgc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaagttgg gtttctatga gtggacatct aggctaagaa gtcacatcaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaatgtctt tgaaagattt gtta       1194

 
<210> 2
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> wild type human alpha-galactosidase A
 
<400> 2
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 3
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 3
ttggataacg ggttagcccg tacacctact atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcatggag 120
atggctgaac ggatggtaag tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aactacgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
gcacccctat tcatgtctaa tgatctacgt cacatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga atggacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaatgtctt tgaaagattt gtta       1194

 
<210> 4
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 4
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 5
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 5
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctataa cgcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 6
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 6
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Asn Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 7
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 7
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 8
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 8
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 9
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 9
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac tgatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg caaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggaa ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatcgc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 10
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 10
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 11
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 11
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac tgatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agacaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 12
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 12
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 13
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 13
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac tgatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggaa ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agacaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 14
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 14
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 15
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 15
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttcttt taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 16
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 16
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 17
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 17
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctatgatca accgtcaaga gatcggaggg ccaagatctt acactatcgc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 18
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 18
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 19
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 19
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac tgatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggaa ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agacaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctatgatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 20
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 20
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 21
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 21
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agacaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctatgatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 22
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 22
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 23
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 23
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctatgatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 24
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 24
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 25
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 25
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agacaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatcgc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 26
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 26
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 27
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 27
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac tgatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttcttt taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctatgatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 28
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 28
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 29
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 29
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac tgatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggaa ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctatgatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 30
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 30
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 31
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 31
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac tgatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg caaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatcgc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 32
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 32
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 33
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 33
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac tgatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 34
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 34
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 35
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 35
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac tgatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggaa ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 36
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 36
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 37
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 37
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttcttt taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctatgatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 38
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 38
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 39
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 39
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac tgatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttcttt taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggattagc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 40
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 40
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 41
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 41
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggaa ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 42
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 42
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 43
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 43
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac tgatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttcttt taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 44
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 44
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 45
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 45
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 46
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 46
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 47
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 47
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggaa ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctatgatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 48
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 48
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 49
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 49
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac tgatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 50
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 50
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 51
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 51
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggattagc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 52
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 52
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 53
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 53
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac tgatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggaa ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 54
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 54
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 55
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 55
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggattagc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatcgc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 56
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 56
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 57
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 57
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 58
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 58
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 59
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 59
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac tgatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttcttt taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctatgatca accgtcaaga gatcggaggg ccaagatctt acactatcgc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 60
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 60
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 61
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 61
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtagggcgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 62
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 62
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ala Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     


<210> 63
<211> 100
<212> DNA
<213> Artificial Sequence
 
<220>
<223> BamHI-pCDNA-GLA-F
 
<400> 63
acgtttggat cccgccacca tggaatggag ctgggtcttt ctcttcttcc tgtcagtaac 60
gactggtgtc cactccttgg ataacgggtt agcccgtaca						  100


<210> 64
<211> 35
<212> DNA
<213> Artificial Sequence
 
<220>
<223> XhoI-pCDNA-GLA-R
 
<400> 64
attgtactcg agttataaca aatctttcaa agaca 35


<210> 65
<211> 57
<212> DNA
<213> Artificial Sequence
 
<220>
<223> synthetic mouse IG signal peptide
 
<400> 65
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactcc 57


<210> 66
<211> 19
<212> PRT
<213> Artificial Sequence
 
<220>
<223> synthetic mouse IG signal peptide
 
<400> 66
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15   
Val His Ser 

<210> 67
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 67
ttggataacg ggttagccct gacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc atttggtcac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 68
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 68
Leu Asp Asn Gly Leu Ala Leu Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly His Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 69
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 69
ttggataacg ggttagccct gacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtgtctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 70
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 70
Leu Asp Asn Gly Leu Ala Leu Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Val Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 71
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 71
ttggataacg ggttagccct gacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tgcccttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc atttggtcac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acgggtttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtgtctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 72
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 72
Leu Asp Asn Gly Leu Ala Leu Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Ala Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly His Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Gly Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Val Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 73
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 73
ttggataacg ggttagccct gacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acgggtttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 74
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 74
Leu Asp Asn Gly Leu Ala Leu Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Gly Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 75
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 75
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tgcccttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc atttggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 76
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 76
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Ala Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 77
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 77
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caaaacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaattttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 78
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 78
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asn Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 79
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 79
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcgtatc 900
agaaaaggtg acaattttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 80
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 80
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Arg Ile Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 81
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 81
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc atttggtcac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 82
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 82
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly His Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 83
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 83
ttggataacg ggttagccct gacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 84
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 84
Leu Asp Asn Gly Leu Ala Leu Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 85
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 85
ttggataacg ggttagccct gacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acgggtttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtgtctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 86
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 86
Leu Asp Asn Gly Leu Ala Leu Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Gly Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Val Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 87
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 87
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc atttggtcac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcgtctg 900
agaaaaggtg acgggtttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 88
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 88
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly His Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Arg Leu Arg Lys Gly Asp 
    290                 295                 300                 
Gly Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 89
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 89
ttggataacg ggttagccct gacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgatggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc agagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc atttggtcac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attcgctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caaaacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcgtctg 900
agaaaaggtg acaattttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 90
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 90
Leu Asp Asn Gly Leu Ala Leu Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Glu Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly His Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asn Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Arg Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 91
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 91
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgatggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 92
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 92
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 93
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 93
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc agagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 94
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 94
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Glu Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 95
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 95
ttggataacg ggttagccct gacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgag tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 96
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 96
Leu Asp Asn Gly Leu Ala Leu Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Glu Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 97
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 97
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctac acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attcgctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgggt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 98
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 98
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Thr Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Gly Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 99
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 99
ttggataacg ggttagccct gacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgatggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attcgctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgggt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 100
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 100
Leu Asp Asn Gly Leu Ala Leu Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Gly Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 101
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 101
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtgtctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 102
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 102
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Val Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 103
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 103
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attcgctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaaatc 900
agaaaaggtg acaattttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 104
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 104
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Ile Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 105
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 105
ttggataacg ggttagccct gacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgatggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc agagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 106
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 106
Leu Asp Asn Gly Leu Ala Leu Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Glu Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 107
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 107
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgatggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc atttggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attcgctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgggt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caaaacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 108
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 108
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Gly Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asn Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 109
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 109
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc atttggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgggt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 110
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 110
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Gly Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 111
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 111
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcgtatc 900
agaaaaggtg acgggtttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtgtctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 112
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 112
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Arg Ile Arg Lys Gly Asp 
    290                 295                 300                 
Gly Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Val Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 113
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 113
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caaaacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtgtctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 114
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 114
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asn Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Val Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 115
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 115
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgatggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caaaacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaactg 900
agaaaaggtg acgggtttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 116
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 116
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asn Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Gly Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 117
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 117
ttggataacg ggttagccct gacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc agagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgggt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 118
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 118
Leu Asp Asn Gly Leu Ala Leu Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Glu Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Gly Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 119
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 119
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaactg 900
agaaaaggtg acgggtttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 120
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 120
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Gly Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 121
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 121
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc agagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attcgctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcgtatc 900
agaaaaggtg acaattttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 122
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 122
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Glu Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Arg Ile Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 123
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 123
ttggataacg ggttagccct gacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgatggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgag tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attcgctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caaaacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 124
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 124
Leu Asp Asn Gly Leu Ala Leu Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Glu Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asn Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 125
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 125
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgatggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc agagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 126
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 126
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Glu Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 127
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 127
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acgggtttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtgtctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 128
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 128
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Gly Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Val Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 129
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 129
ttggataacg ggttagccct gacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caaaacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 130
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 130
Leu Asp Asn Gly Leu Ala Leu Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asn Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 131
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 131
ttggataacg ggttagccct gacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc agagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgag tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caaaacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtgtctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 132
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 132
Leu Asp Asn Gly Leu Ala Leu Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Glu Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Glu Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asn Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Val Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 133
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 133
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgatggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caaaacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaactg 900
agaaaaggtg acaattttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 134
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 134
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asn Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 135
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 135
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catggtcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaaag gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 136
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 136
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ser Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 137
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 137
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aaccgtactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtgacg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 138
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 138
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Asp Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 139
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 139
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtggcg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 140
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 140
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Gly Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 141
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 141
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg ccagcttgca aatcatgtac acagtctggg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 142
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 142
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Leu 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 143
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 143
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagtctggg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agacaattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtggcg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 144
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 144
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Leu 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Gln Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Gly Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 145
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 145
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aatcatgtac acagtctggg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataccgc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaaag gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 146
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 146
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Leu 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Pro Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 147
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 147
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tgcagctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataggcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaaag gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 148
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 148
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Ala Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Gly Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 149
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 149
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tcgtgctcta 480
aaccgtactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 150
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 150
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Arg Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 151
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 151
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg ccagcttgca cagcatgtac acgagaaggg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaaag gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 152
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 152
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Gln His Val His Glu Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 153
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 153
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg ccagcttgca cagcatgtac acgagctggg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaaag gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 154
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 154
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Gln His Val His Glu Leu 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 155
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 155
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aaccatactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtgacg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 156
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 156
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn His Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Asp Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 157
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 157
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 158
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 158
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 159
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 159
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataccgc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaaag gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 160
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 160
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Pro Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 161
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 161
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacagtactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 162
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 162
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Ser Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 163
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 163
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcataaa gcagcttgca aatcatgtac acagtattgg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aaaacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataccgc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtggcg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 164
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 164
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Lys Gln Leu Ala Asn His Val His Ser Ile 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys Asn Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Pro Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Gly Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 165
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 165
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcataaa gcagcttgca cagcatgtac acgagctggg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catggtcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtggcg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 166
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 166
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Lys Gln Leu Ala Gln His Val His Glu Leu 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ser Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Gly Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 167
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 167
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcataaa gcagcttgca cagcatgtac acgagctggg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tgcagctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 168
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 168
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Lys Gln Leu Ala Gln His Val His Glu Leu 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Ala Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 169
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 169
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataccgc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 170
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 170
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Pro Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 171
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 171
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataccgc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtggcg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 172
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 172
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Pro Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Gly Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 173
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 173
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaga cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaaag gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 174
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 174
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Asp Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 175
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 175
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaga cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtggcg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 176
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 176
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Asp Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Gly Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 177
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 177
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaga cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcataaa gcagcttgca aatcatgtac acgagctggg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tcgtgctcta 480
aaccatactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 178
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 178
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Asp Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Lys Gln Leu Ala Asn His Val His Glu Leu 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Arg Ala Leu 
145                 150                 155                 160 
Asn His Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 179
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 179
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaga cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataccgc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 180
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 180
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Asp Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Pro Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 181
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 181
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaga cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg ccagcttgca aatcatgtac acgagaaggg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aaccgtactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaaag gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 182
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 182
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Asp Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Glu Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 183
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 183
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat ggggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 184
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 184
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Gly Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 185
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 185
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacaagatg caaacctctt tgaaagattt gttataa    1197

 
<210> 186
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 186
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Lys Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 187
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 187
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgacg 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 188
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 188
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Thr Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 189
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 189
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtgcgtatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 190
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 190
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Ala Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 191
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 191
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccggtt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 192
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 192
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Val Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 193
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 193
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tgcggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 194
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 194
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Ala Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 195
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 195
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgctg 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 196
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 196
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Leu Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 197
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 197
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaaccggt aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 198
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 198
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Gly Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 199
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 199
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtcttaa    1197

 
<210> 200
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 200
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Ser         
385                 390                 395                     

 
<210> 201
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 201
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagatgc tttataa    1197

 
<210> 202
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 202
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Ala Leu         
385                 390                 395                     

 
<210> 203
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 203
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catggaagtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 204
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 204
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 205
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 205
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggaagat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 206
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 206
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Lys Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 207
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 207
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt aggatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 208
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 208
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Arg Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 209
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 209
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaaggttt aactcga    1197

 
<210> 210
<211> 399
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 210
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Gly Leu Thr Arg     
385                 390                 395                     

 
<210> 211
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 211
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gagtccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 212
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 212
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Ser Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 213
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 213
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgg cgggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 214
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 214
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ala Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 215
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 215
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat ccggaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 216
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 216
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Pro Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 217
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 217
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctcta agaaagattt gttataa    1197

 
<210> 218
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 218
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Lys Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 219
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 219
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
gctaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 220
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 220
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Ala Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 221
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 221
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgcggcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 222
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 222
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Ala Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 223
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 223
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatattc acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 224
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 224
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Ile His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 225
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 225
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtcagtatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 226
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 226
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Gln Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 227
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 227
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactgtgcc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 228
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 228
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Val 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 229
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 229
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aactttgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 230
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 230
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Phe Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 231
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 231
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt ggtgtaa    1197

 
<210> 232
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 232
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Val         
385                 390                 395                     

 
<210> 233
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 233
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gccgtaa    1197

 
<210> 234
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 234
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Pro         
385                 390                 395                     

 
<210> 235
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 235
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
atgaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 236
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 236
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Met Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 237
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 237
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccg ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 238
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 238
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Arg Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 239
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 239
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagagggt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 240
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 240
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 241
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 241
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaacagg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 242
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 242
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Gln Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 243
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 243
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtagt ggaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 244
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 244
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Val Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 245
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 245
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aacacggtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 246
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 246
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Thr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 247
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 247
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccgttt tgaaagattt gttataa    1197

 
<210> 248
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 248
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 249
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 249
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc ctggggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 250
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 250
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Leu Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 251
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 251
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggt 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatttt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 252
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 252
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Phe Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 253
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 253
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcctg gtaaacaagg ttatcaatta 900
agaaagggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg gctacctctt tgaaagattt gttataa    1197

 
<210> 254
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 254
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Pro Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Ala Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 255
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 255
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtcaggcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 256
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 256
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Gln Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 257
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 257
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgtggag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 258
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 258
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Val Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 259
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 259
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctaa gaaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 260
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 260
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Lys Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 261
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 261
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt ggcttaa    1197

 
<210> 262
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 262
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Ala         
385                 390                 395                     

 
<210> 263
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 263
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgacggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 264
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 264
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 265
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 265
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgacggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc agagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 266
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 266
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Glu Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 267
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 267
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgacggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc agagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctaa gaaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagattct acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg gcgaccgtat tgaaagattt gttataa    1197

 
<210> 268
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 268
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Glu Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Lys Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Phe Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Ala Thr Val Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 269
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 269
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgacggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc agagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctctgg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagattct acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaactgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 270
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 270
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Glu Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Phe Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 271
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 271
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgacggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctaa gaaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagaagtt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 272
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 272
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Lys Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 273
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 273
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgacggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagattct acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 274
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 274
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Phe Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 275
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 275
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgacggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagaggtt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 276
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 276
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 277
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 277
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgacggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagaagtt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg cagaccgtat tgaaagattt gttataa    1197

 
<210> 278
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 278
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 279
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 279
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgacggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtcaagcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg cagaccgtat tgaaagattt gttataa    1197

 
<210> 280
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 280
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Gln Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 281
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 281
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc agagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 282
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 282
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Glu Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 283
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 283
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc agagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtcaagcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 284
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 284
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Glu Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Gln Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 285
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 285
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctaa gaaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagattct acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 286
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 286
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Lys Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Phe Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 287
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 287
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctaa gaaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagaggtt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg cagaccagct tgaaagattt gttataa    1197

 
<210> 288
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 288
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Lys Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 289
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 289
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagattct acactatccc ggtagcctct 1020
ttgggtaagg gtcaagcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 290
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 290
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Phe Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Gln Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 291
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 291
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagaggtt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 292
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 292
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 293
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 293
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgag tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 294
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 294
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Glu Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 295
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 295
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaggag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc ctggggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catggtcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caaaacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
cgtaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccg tgtagcctct 1020
ttgggtgatg gtcaggcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccagct tgaaagattt ggcgtaa    1197

 
<210> 296
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 296
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Leu Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ser Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asn Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Arg Val Ala Ser Leu Gly Asp Gly Gln Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Ala         
385                 390                 395                     

 
<210> 297
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 297
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaggag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgag tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc tgtagcctct 1020
ttgggtaagg gtcaggcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccgtat tgaaagattt gctgtaa    1197

 
<210> 298
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 298
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Glu Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Gln Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 299
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 299
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgtggag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccg tgtagcctct 1020
ttgggtggtg gtgtcgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt ggcgtaa    1197

 
<210> 300
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 300
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Val Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Arg Val Ala Ser Leu Gly Gly Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Ala         
385                 390                 395                     

 
<210> 301
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 301
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtagt tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc ctggggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
cgtaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 302
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 302
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Val Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Leu Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 303
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 303
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtagt tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgag tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 304
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 304
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Val Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Glu Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 305
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 305
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtagt tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtgatg gtcaggcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccgtat tgaaagattt gctgtaa    1197

 
<210> 306
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 306
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Val Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Asp Gly Gln Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 307
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 307
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaggag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catggtcgtc aatcaaatct atcttggatt ggacttctaa gaaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
cgtaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 308
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 308
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ser Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Lys Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 309
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 309
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccg tgtagcctct 1020
ttgggtggtg gtcaggcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccagct tgaaagattt ggcgtaa    1197

 
<210> 310
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 310
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Arg Val Ala Ser Leu Gly Gly Gly Gln Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Ala         
385                 390                 395                     

 
<210> 311
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 311
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caaaacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccgtat tgaaagattt gctgtaa    1197

 
<210> 312
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 312
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asn Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 313
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 313
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcgtc aatcaaatct atcttggatt ggacttctcg caaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtgatg gtgtcgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 314
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 314
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Asp Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 315
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 315
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg caaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtgatg gtcaggcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccgtat tgaaagattt ggcgtaa    1197

 
<210> 316
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 316
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Asp Gly Gln Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Ala         
385                 390                 395                     

 
<210> 317
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 317
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgtggag 120
atggctgaac ggatggtagt tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctaa gaaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccagct tgaaagattt gctgtaa    1197

 
<210> 318
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 318
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Val Glu Met Ala Glu Arg Met Val Val Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Lys Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 319
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 319
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catggtcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgtcgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 320
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 320
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ser Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 321
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 321
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc tgtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccgtat tgaaagattt ggcgtaa    1197

 
<210> 322
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 322
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Ala         
385                 390                 395                     

 
<210> 323
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 323
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgtggag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caaaacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccg tgtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt ggcgtaa    1197

 
<210> 324
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 324
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Val Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asn Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Arg Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Ala         
385                 390                 395                     

 
<210> 325
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 325
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc ctggggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 326
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 326
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Leu Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 327
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 327
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg caaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccgtat tgaaagattt gctgtaa    1197

 
<210> 328
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 328
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 329
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 329
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaggag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctaa gaaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccg tgtagcctct 1020
ttgggtaagg gtcaggcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccagct tgaaagattt ggcgtaa    1197

 
<210> 330
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 330
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Lys Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Arg Val Ala Ser Leu Gly Lys Gly Gln Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Ala         
385                 390                 395                     

 
<210> 331
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 331
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc ctggggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caaaacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccgtat tgaaagattt gctgtaa    1197

 
<210> 332
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 332
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Leu Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asn Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 333
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 333
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgtggag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccgtat tgaaagattt ggcgtaa    1197

 
<210> 334
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 334
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Val Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Ala         
385                 390                 395                     

 
<210> 335
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 335
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tcgtgctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc tgtagcctct 1020
ttgggtaagg gtgtcgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 336
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 336
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Arg Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 337
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 337
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc ctggggtaca aacacatgag tcgtgctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctaa gaaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc tgtagcctct 1020
ttgggtggtg gtcaggcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccgtat tgaaagattt gctgtaa    1197

 
<210> 338
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 338
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Leu Gly Tyr Lys His Met Ser Arg Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Lys Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Gly Gly Gln Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 339
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 339
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaggag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgag tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tcgtgctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg caaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 340
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 340
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Glu Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Arg Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 341
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 341
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc ctggggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccgtat tgaaagattt gctgtaa    1197

 
<210> 342
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 342
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Leu Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 343
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 343
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaggag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctaa gaaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 344
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 344
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Lys Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 345
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 345
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgag tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tcgtgctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg caaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccgtat tgaaagattt gctgtaa    1197

 
<210> 346
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 346
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Glu Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Arg Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 347
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 347
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgtggag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc ctggggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 348
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 348
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Val Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Leu Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 349
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 349
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc ctggggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc tgtagcctct 1020
ttgggtaagg gtcaggcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccgtat tgaaagattt ggcgtaa    1197

 
<210> 350
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 350
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Leu Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Gln Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Ala         
385                 390                 395                     

 
<210> 351
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 351
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgtggag 120
atggctgaac ggatggtaga tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 352
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 352
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Val Glu Met Ala Glu Arg Met Val Asp Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 353
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 353
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tcgtgctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccgtat tgaaagattt gctgtaa    1197

 
<210> 354
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 354
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Arg Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 355
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 355
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctagcgatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctacagcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagaagct acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 356
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 356
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Ala Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 357
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 357
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt aatataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg cagacctctt tgaaagattt gttataa    1197

 
<210> 358
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 358
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Asn Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 359
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 359
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
ataaatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg cagacctctt tgaaagattt gttataa    1197

 
<210> 360
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 360
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asn Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 361
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 361
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttat cgtgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
ataaatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 362
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 362
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Ser Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asn Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 363
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 363
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagaaact acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 364
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 364
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Asn Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 365
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 365
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgcatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctacagcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagaaact acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 366
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 366
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Asn Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 367
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 367
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctacagcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt aatataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagaagct acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 368
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 368
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Asn Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 369
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 369
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctacagcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagaagct acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgaa caacacaatg cagacctctt tgaaagattt gttataa    1197

 
<210> 370
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 370
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Asn Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 371
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 371
ctggacaatg gattggcaag gacgcctacc atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 372
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 372
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 373
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 373
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagacgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 374
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 374
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 375
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 375
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatctag gaaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatccc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga agctacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 376
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 376
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 377
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 377
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaagag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 378
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 378
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 379
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 379
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 380
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 380
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 381
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 381
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 382
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 382
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 383
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 383
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctgggccag tataaagagt atcttggact ggacatctag gaaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagacctcat taaaagactt actt       1194

 
<210> 384
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 384
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 385
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 385
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc ggatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctgggccag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatccc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 386
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 386
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 387
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 387
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc ggatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
ggtcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 388
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 388
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 389
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 389
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc ggatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
ggtcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 390
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 390
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 391
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 391
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc ggatggtcac tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctgggccag tataaagagt atcttggact ggacatctag gaaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcctcgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 392
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 392
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 393
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 393
ctggacaatg gattggcaag gacgcctacc atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc ggatggtcac tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcctcgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga agctacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 394
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 394
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 395
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 395
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc ggatggtcac tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 396
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 396
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 397
<211> 1251
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 397
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctacaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt catggagatg 180
gcagagcgga tggtcactga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat catgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct gggccagtat aaagagtatc ttggactgga catctaggaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctggaacca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
cagggagaca actttgaagt gtgggaacga cctctctcag gcgatgcctg ggctgtagct 1020
attataaacc ggcaggagat tggtggacct cgctcttata ccatcgcagt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaatg gacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t          1251

 
<210> 398
<211> 417
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 398
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp 
            20                  25                  30          
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro 
        35                  40                  45              
Asp Ser Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met 
    50                  55                  60                  
Val Thr Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp 
65                  70                  75                  80  
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala 
                85                  90                  95      
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val 
            100                 105                 110         
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys 
        115                 120                 125             
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala 
    130                 135                 140                 
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys 
145                 150                 155                 160 
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser 
                165                 170                 175     
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp 
            180                 185                 190         
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg 
        195                 200                 205             
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp 
    210                 215                 220                 
Ala Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg 
225                 230                 235                 240 
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu 
                245                 250                 255     
Val Ile Gly Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met 
            260                 265                 270         
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu 
        275                 280                 285             
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val 
    290                 295                 300                 
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg 
305                 310                 315                 320 
Gln Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala 
                325                 330                 335     
Trp Ala Val Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser 
            340                 345                 350         
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro 
        355                 360                 365             
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe 
    370                 375                 380                 
Tyr Glu Trp Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr 
385                 390                 395                 400 
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu 
                405                 410                 415     
Leu                                                             
                                                                

 
<210> 399
<211> 1251
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 399
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctacaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt catggagatg 180
gcagagcgga tggtcactga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat catgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaaaagtat aaagagtatc ttggactgga catcttttaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctgggacca gcaagtaact cagatggccc tctgggctat catggctggt 840
cctttattca tgtctaatga cctccgagcc atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
cagggagaca actttgaagt gtgggaacga cctctctcag gcgatgcctg ggctgtagct 1020
atgataaacc ggcaggagat tggtggacct cgctcttata ccatccccgt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaagc tacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t          1251

 
<210> 400
<211> 417
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 400
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp 
            20                  25                  30          
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro 
        35                  40                  45              
Asp Ser Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met 
    50                  55                  60                  
Val Thr Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp 
65                  70                  75                  80  
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala 
                85                  90                  95      
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val 
            100                 105                 110         
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys 
        115                 120                 125             
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala 
    130                 135                 140                 
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys 
145                 150                 155                 160 
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser 
                165                 170                 175     
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp 
            180                 185                 190         
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg 
        195                 200                 205             
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp 
    210                 215                 220                 
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg 
225                 230                 235                 240 
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu 
                245                 250                 255     
Val Ile Gly Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met 
            260                 265                 270         
Ala Leu Trp Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu 
        275                 280                 285             
Arg Ala Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val 
    290                 295                 300                 
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg 
305                 310                 315                 320 
Gln Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala 
                325                 330                 335     
Trp Ala Val Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser 
            340                 345                 350         
Tyr Thr Ile Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro 
        355                 360                 365             
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe 
    370                 375                 380                 
Tyr Glu Ala Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr 
385                 390                 395                 400 
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu 
                405                 410                 415     
Leu                                                             
                                                                

 
<210> 401
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 401
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc ggatggtctc tgaaggctgg aaggatgcag gttatgagta cctatgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctgggccag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga agctacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 402
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 402
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 403
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 403
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagatagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaagag tataaagagt atcttggact ggacatctag gaaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
ggtcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 404
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 404
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Ile Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 405
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 405
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcgaggag 120
atggcagagc ggatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaagag tataaagagt atcttggact ggacatctag gaaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcctcgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 406
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 406
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 407
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 407
ctggacaatg gattggcaag gacgcctacc atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcgaggag 120
atggcagagc ggatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 408
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 408
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 409
<211> 1251
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 409
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctacaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt cgaggagatg 180
gcagagcgga tggtctctga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat catgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
atgactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaaaagtat aaagagtatc ttggactgga catctttcaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctgggacca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
aagggagaca actttgaagt gtgggaacga cctctctcag gcgatgcctg ggctgtagct 1020
attataaacc ggcaggagat tggtggacct cgctcttata ccatcgccgt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaatg gacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t          1251

 
<210> 410
<211> 417
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 410
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp 
            20                  25                  30          
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro 
        35                  40                  45              
Asp Ser Cys Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met 
    50                  55                  60                  
Val Ser Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp 
65                  70                  75                  80  
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala 
                85                  90                  95      
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val 
            100                 105                 110         
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys 
        115                 120                 125             
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala 
    130                 135                 140                 
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys 
145                 150                 155                 160 
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser 
                165                 170                 175     
Leu Ala Leu Asn Met Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp 
            180                 185                 190         
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg 
        195                 200                 205             
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp 
    210                 215                 220                 
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg 
225                 230                 235                 240 
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu 
                245                 250                 255     
Val Ile Gly Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met 
            260                 265                 270         
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu 
        275                 280                 285             
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val 
    290                 295                 300                 
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg 
305                 310                 315                 320 
Lys Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala 
                325                 330                 335     
Trp Ala Val Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser 
            340                 345                 350         
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro 
        355                 360                 365             
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe 
    370                 375                 380                 
Tyr Glu Trp Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr 
385                 390                 395                 400 
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu 
                405                 410                 415     
Leu                                                             
                                                                

 
<210> 411
<211> 1251
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 411
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctacaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt cgaggagatg 180
gcagagctga tggtctctga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat tatgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaagagtat aaagagtatc ttggactgga catcttttaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctggaacca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
cagggagaca actttgaagt gtgggaacga cctctctcag gcctcgcctg ggctgtagct 1020
attataaacc ggcaggagat tggtggacct cgctcttata ccatcgcagt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaatg gacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t          1251

 
<210> 412
<211> 417
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 412
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp 
            20                  25                  30          
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro 
        35                  40                  45              
Asp Ser Cys Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met 
    50                  55                  60                  
Val Ser Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp 
65                  70                  75                  80  
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala 
                85                  90                  95      
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val 
            100                 105                 110         
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys 
        115                 120                 125             
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala 
    130                 135                 140                 
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys 
145                 150                 155                 160 
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser 
                165                 170                 175     
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp 
            180                 185                 190         
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg 
        195                 200                 205             
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp 
    210                 215                 220                 
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg 
225                 230                 235                 240 
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu 
                245                 250                 255     
Val Ile Gly Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met 
            260                 265                 270         
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu 
        275                 280                 285             
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val 
    290                 295                 300                 
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg 
305                 310                 315                 320 
Gln Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala 
                325                 330                 335     
Trp Ala Val Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser 
            340                 345                 350         
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro 
        355                 360                 365             
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe 
    370                 375                 380                 
Tyr Glu Trp Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr 
385                 390                 395                 400 
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu 
                405                 410                 415     
Leu                                                             
                                                                

 
<210> 413
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 413
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcgaggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 414
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 414
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 415
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 415
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcgaggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctcttt caggcgatgc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 416
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 416
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 417
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 417
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcgaggag 120
atggcagagc tgatggtcac tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatctag gaaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga agctacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 418
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 418
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 419
<211> 1251
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 419
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctacaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt cgaggagatg 180
gcagagctga tggtcactga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat tatgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaaaagtat aaagagtatc ttggactgga catctttcaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctgggacca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
cagggagaca actttgaagt gtgggaacga cctctctcag gcctcgcctg ggctgtagct 1020
atgataaacc ggcaggagat tggtggacct cgctcttata ccatcgcagt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaatg gacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t          1251

 
<210> 420
<211> 417
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 420
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp 
            20                  25                  30          
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro 
        35                  40                  45              
Asp Ser Cys Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met 
    50                  55                  60                  
Val Thr Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp 
65                  70                  75                  80  
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala 
                85                  90                  95      
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val 
            100                 105                 110         
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys 
        115                 120                 125             
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala 
    130                 135                 140                 
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys 
145                 150                 155                 160 
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser 
                165                 170                 175     
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp 
            180                 185                 190         
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg 
        195                 200                 205             
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp 
    210                 215                 220                 
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg 
225                 230                 235                 240 
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu 
                245                 250                 255     
Val Ile Gly Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met 
            260                 265                 270         
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu 
        275                 280                 285             
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val 
    290                 295                 300                 
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg 
305                 310                 315                 320 
Gln Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala 
                325                 330                 335     
Trp Ala Val Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser 
            340                 345                 350         
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro 
        355                 360                 365             
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe 
    370                 375                 380                 
Tyr Glu Trp Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr 
385                 390                 395                 400 
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu 
                405                 410                 415     
Leu                                                             
                                                                

 
<210> 421
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 421
ctggacaatg gattggcaag gacgcctacc atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcgaggag 120
atggcagagc tgatggtcac tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 422
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 422
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 423
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 423
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcgaggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 424
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 424
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 425
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 425
ctggacaatg gattggcaag gacgcctacc atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcgaggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatccc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga agctacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 426
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 426
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 427
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 427
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 428
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 428
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 429
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 429
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 430
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 430
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 431
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 431
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 432
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 432
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     
 
<210> 433
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 433
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 434
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 434
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 435
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 435
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcctcgc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 436
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 436
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 437
<211> 1251
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 437
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctacaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt catggagatg 180
gcagagctga tggtctctga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat tatgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaaaagtat aaagagtatc ttggactgga catctttcaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctggaacca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
aagggagaca actttgaagt gtgggaacga cctctctcag gcttagcctg ggctgtagct 1020
attataaacc ggcaggagat tggtggacct cgctcttata ccatcgcagt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaagc tacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t          1251

 
<210> 438
<211> 417
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 438
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp 
            20                  25                  30          
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro 
        35                  40                  45              
Asp Ser Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met 
    50                  55                  60                  
Val Ser Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp 
65                  70                  75                  80  
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala 
                85                  90                  95      
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val 
            100                 105                 110         
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys 
        115                 120                 125             
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala 
    130                 135                 140                 
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys 
145                 150                 155                 160 
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser 
                165                 170                 175     
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp 
            180                 185                 190         
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg 
        195                 200                 205             
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp 
    210                 215                 220                 
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg 
225                 230                 235                 240 
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu 
                245                 250                 255     
Val Ile Gly Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met 
            260                 265                 270         
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu 
        275                 280                 285             
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val 
    290                 295                 300                 
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg 
305                 310                 315                 320 
Lys Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala 
                325                 330                 335     
Trp Ala Val Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser 
            340                 345                 350         
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro 
        355                 360                 365             
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe 
    370                 375                 380                 
Tyr Glu Ala Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr 
385                 390                 395                 400 
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu 
                405                 410                 415     
Leu                                                             
                                                                

 
<210> 439
<211> 1251
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 439
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctacaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt catggagatg 180
gcagagctga tggtcactga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat tatgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaaaagtat aaagagtatc ttggactgga catctaggaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctggaatca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
aagggagaca actttgaagt gtgggaacga cctctctcag gcttagcctg ggctgtagct 1020
atgataaacc ggcaggagat tggtggacct cgctcttata ccatcgcagt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaatg gacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t          1251

 
<210> 440
<211> 417
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 440
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp 
            20                  25                  30          
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro 
        35                  40                  45              
Asp Ser Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met 
    50                  55                  60                  
Val Thr Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp 
65                  70                  75                  80  
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala 
                85                  90                  95      
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val 
            100                 105                 110         
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys 
        115                 120                 125             
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala 
    130                 135                 140                 
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys 
145                 150                 155                 160 
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser 
                165                 170                 175     
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp 
            180                 185                 190         
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg 
        195                 200                 205             
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp 
    210                 215                 220                 
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg 
225                 230                 235                 240 
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu 
                245                 250                 255     
Val Ile Gly Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met 
            260                 265                 270         
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu 
        275                 280                 285             
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val 
    290                 295                 300                 
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg 
305                 310                 315                 320 
Lys Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala 
                325                 330                 335     
Trp Ala Val Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser 
            340                 345                 350         
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro 
        355                 360                 365             
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe 
    370                 375                 380                 
Tyr Glu Trp Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr 
385                 390                 395                 400 
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu 
                405                 410                 415     
Leu                                                             
                                                                

 
<210> 441
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 441
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtcac tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 442
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 442
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 443
<211> 1251
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 443
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctacaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt catggagatg 180
gcagagctga tggtcactga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat tatgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaaaagtat aaagagtatc ttggactgga catctttcaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctgggacca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgagcc atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
cagggagaca actttgaagt gtgggaacga cctctctcag gcttagcctg ggctgtagct 1020
atgataaacc ggcaggagat tggtggacct cgctcttata ccatcgcagt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaatg gacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t          1251

 
<210> 444
<211> 417
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 444
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp 
            20                  25                  30          
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro 
        35                  40                  45              
Asp Ser Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met 
    50                  55                  60                  
Val Thr Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp 
65                  70                  75                  80  
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala 
                85                  90                  95      
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val 
            100                 105                 110         
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys 
        115                 120                 125             
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala 
    130                 135                 140                 
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys 
145                 150                 155                 160 
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser 
                165                 170                 175     
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp 
            180                 185                 190         
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg 
        195                 200                 205             
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp 
    210                 215                 220                 
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg 
225                 230                 235                 240 
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu 
                245                 250                 255     
Val Ile Gly Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met 
            260                 265                 270         
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu 
        275                 280                 285             
Arg Ala Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val 
    290                 295                 300                 
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg 
305                 310                 315                 320 
Gln Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala 
                325                 330                 335     
Trp Ala Val Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser 
            340                 345                 350         
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro 
        355                 360                 365             
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe 
    370                 375                 380                 
Tyr Glu Trp Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr 
385                 390                 395                 400 
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu 
                405                 410                 415     
Leu                                                             
                                                                

 
<210> 445
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 445
ctggacaatg gattggcaag gacgcctacc atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtcac tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 446
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 446
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 447
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 447
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 448
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 448
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 449
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 449
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa ccagcaagta actcagatgg ccctctgggc tatcatggct 780
ggtcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 450
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 450
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 451
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 451
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaagag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatccc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagacctcat taaaagactt actt       1194

 
<210> 452
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 452
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 453
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 453
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 454
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 454
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 455
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 455
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctgggccag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa ccagcaagta actcagatgg ccctctgggc tatcatggct 780
ggtcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 456
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 456
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 457
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 457
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctgggccag tataaagagt atcttggact ggacatctag gaaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcctcgc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 458
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 458
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 459
<211> 1251
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 459
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctccaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt catggagatg 180
gcagagctca tggtctcaga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat tatgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct gggccagtat aaagagtatc ttggactgga catcttttaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctgggacca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
cagggagaca actttgaagt gtgggaacga cctctctcag gcttagcctg ggctgtagct 1020
atgataaacc ggcaggagat tggtggacct cgctcttata ccatcgcagt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaatg gacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t          1251

 
<210> 460
<211> 417
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 460
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp 
            20                  25                  30          
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro 
        35                  40                  45              
Asp Ser Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met 
    50                  55                  60                  
Val Ser Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp 
65                  70                  75                  80  
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala 
                85                  90                  95      
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val 
            100                 105                 110         
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys 
        115                 120                 125             
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala 
    130                 135                 140                 
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys 
145                 150                 155                 160 
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser 
                165                 170                 175     
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp 
            180                 185                 190         
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg 
        195                 200                 205             
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp 
    210                 215                 220                 
Ala Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg 
225                 230                 235                 240 
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu 
                245                 250                 255     
Val Ile Gly Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met 
            260                 265                 270         
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu 
        275                 280                 285             
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val 
    290                 295                 300                 
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg 
305                 310                 315                 320 
Gln Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala 
                325                 330                 335     
Trp Ala Val Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser 
            340                 345                 350         
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro 
        355                 360                 365             
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe 
    370                 375                 380                 
Tyr Glu Trp Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr 
385                 390                 395                 400 
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu 
                405                 410                 415     
Leu                                                             
                                                                

 
<210> 461
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 461
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctact ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 462
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 462
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 463
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 463
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc ggatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 464
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 464
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 465
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 465
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc ggatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaagag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
ggtcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 466
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 466
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 467
<211> 1251
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 467
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctccaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt catggagatg 180
gcagagcgga tggtctctga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat tatgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaaaagtat aaagagtatc ttggactgga catctttcaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctggaatca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
aagggagaca actttgaagt gtgggaacga cctctctcag gcttagcctg ggctgtagct 1020
atgataaacc ggcaggagat tggtggacct cgctcttata ccatccccgt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaagc tacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t          1251

 
<210> 468
<211> 417
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 468
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp 
            20                  25                  30          
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro 
        35                  40                  45              
Asp Ser Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met 
    50                  55                  60                  
Val Ser Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp 
65                  70                  75                  80  
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala 
                85                  90                  95      
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val 
            100                 105                 110         
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys 
        115                 120                 125             
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala 
    130                 135                 140                 
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys 
145                 150                 155                 160 
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser 
                165                 170                 175     
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp 
            180                 185                 190         
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg 
        195                 200                 205             
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp 
    210                 215                 220                 
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg 
225                 230                 235                 240 
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu 
                245                 250                 255     
Val Ile Gly Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met 
            260                 265                 270         
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu 
        275                 280                 285             
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val 
    290                 295                 300                 
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg 
305                 310                 315                 320 
Lys Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala 
                325                 330                 335     
Trp Ala Val Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser 
            340                 345                 350         
Tyr Thr Ile Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro 
        355                 360                 365             
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe 
    370                 375                 380                 
Tyr Glu Ala Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr 
385                 390                 395                 400 
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu 
                405                 410                 415     
Leu                                                             
                                                                

 
<210> 469
<211> 1251
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 469
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctccaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt catggagatg 180
gcagagcgga tggtcactga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat tatgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaaaagtat aaagagtatc ttggactgga catcttttaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctggaacca gcaagtaact cagatggccc tctgggctat catggctggt 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
aagggagaca actttgaagt gtgggaacga cctctctcag gcctcgcctg ggctgtagct 1020
attataaacc ggcaggagat tggtggacct cgctcttata ccatcgccgt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaagc tacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t          1251

 
<210> 470
<211> 417
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 470
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp 
            20                  25                  30          
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro 
        35                  40                  45              
Asp Ser Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met 
    50                  55                  60                  
Val Thr Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp 
65                  70                  75                  80  
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala 
                85                  90                  95      
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val 
            100                 105                 110         
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys 
        115                 120                 125             
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala 
    130                 135                 140                 
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys 
145                 150                 155                 160 
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser 
                165                 170                 175     
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp 
            180                 185                 190         
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg 
        195                 200                 205             
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp 
    210                 215                 220                 
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg 
225                 230                 235                 240 
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu 
                245                 250                 255     
Val Ile Gly Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met 
            260                 265                 270         
Ala Leu Trp Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu 
        275                 280                 285             
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val 
    290                 295                 300                 
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg 
305                 310                 315                 320 
Lys Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala 
                325                 330                 335     
Trp Ala Val Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser 
            340                 345                 350         
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro 
        355                 360                 365             
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe 
    370                 375                 380                 
Tyr Glu Ala Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr 
385                 390                 395                 400 
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu 
                405                 410                 415     
Leu                                                             
                                                                

 
<210> 471
<211> 1251
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 471
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctccaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt catggagatg 180
gcagagcgga tggtcactga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat catgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaagagtat aaagagtatc ttggactgga catctttcaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctgggacca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
cagggagaca actttgaagt gtgggaacga cctctctcag gcttagcctg ggctgtagct 1020
atgataaacc ggcaggagat tggtggacct cgctcttata ccatcgcagt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaatg gacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t          1251

 
<210> 472
<211> 417
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 472
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp 
            20                  25                  30          
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro 
        35                  40                  45              
Asp Ser Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met 
    50                  55                  60                  
Val Thr Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp 
65                  70                  75                  80  
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala 
                85                  90                  95      
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val 
            100                 105                 110         
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys 
        115                 120                 125             
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala 
    130                 135                 140                 
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys 
145                 150                 155                 160 
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser 
                165                 170                 175     
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp 
            180                 185                 190         
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg 
        195                 200                 205             
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp 
    210                 215                 220                 
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg 
225                 230                 235                 240 
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu 
                245                 250                 255     
Val Ile Gly Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met 
            260                 265                 270         
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu 
        275                 280                 285             
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val 
    290                 295                 300                 
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg 
305                 310                 315                 320 
Gln Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala 
                325                 330                 335     
Trp Ala Val Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser 
            340                 345                 350         
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro 
        355                 360                 365             
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe 
    370                 375                 380                 
Tyr Glu Trp Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr 
385                 390                 395                 400 
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu 
                405                 410                 415     
Leu                                                             
                                                                

 
<210> 473
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 473
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc ggatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaagag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 474
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 474
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 475
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 475
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcgaggag 120
atggcagagc ggatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaagag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcctcgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 476
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 476
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 477
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 477
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcgaggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctgggccag tataaagagt atcttggact ggacatctag gaaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 478
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 478
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 479
<211> 1251
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 479
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctccaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt cgaggagatg 180
gcagagctga tggtctctga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat catgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaaaagtat aaagagtatc ttggactgga catctttcaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctgggacca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
cagggagaca actttgaagt gtgggaacga cctctctcag gcttagcctg ggctgtagct 1020
atgataaacc ggcaggagat tggtggacct cgctcttata ccatcgcagt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaatg gacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t          1251

 
<210> 480
<211> 417
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 480
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp 
            20                  25                  30          
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro 
        35                  40                  45              
Asp Ser Cys Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met 
    50                  55                  60                  
Val Ser Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp 
65                  70                  75                  80  
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala 
                85                  90                  95      
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val 
            100                 105                 110         
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys 
        115                 120                 125             
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala 
    130                 135                 140                 
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys 
145                 150                 155                 160 
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser 
                165                 170                 175     
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp 
            180                 185                 190         
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg 
        195                 200                 205             
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp 
    210                 215                 220                 
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg 
225                 230                 235                 240 
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu 
                245                 250                 255     
Val Ile Gly Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met 
            260                 265                 270         
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu 
        275                 280                 285             
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val 
    290                 295                 300                 
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg 
305                 310                 315                 320 
Gln Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala 
                325                 330                 335     
Trp Ala Val Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser 
            340                 345                 350         
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro 
        355                 360                 365             
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe 
    370                 375                 380                 
Tyr Glu Trp Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr 
385                 390                 395                 400 
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu 
                405                 410                 415     
Leu                                                             
                                                                

 
<210> 481
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 481
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcgaggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaagag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 482
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 482
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 483
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 483
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 484
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 484
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 485
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 485
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 486
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 486
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 487
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 487
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcctcgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatccc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 488
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 488
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 489
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 489
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtcac tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaagag tataaagagt atcttggact ggacatctag gaaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcctcgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 490
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 490
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 491
<211> 1251
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 491
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctccaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt catggagatg 180
gcagagctca tggtcactga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat tatgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaaaagtat aaagagtatc ttggactgga catctaggaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctgggacca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
cagggagaca actttgaagt gtgggaacga cctctctcag gcgatgcctg ggctgtagct 1020
atgataaacc ggcaggagat tggtggacct cgctcttata ccatcgcagt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaatg gacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag acctcattaa aagacttact t          1251

 
<210> 492
<211> 417
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 492
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp 
            20                  25                  30          
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro 
        35                  40                  45              
Asp Ser Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met 
    50                  55                  60                  
Val Thr Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp 
65                  70                  75                  80  
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala 
                85                  90                  95      
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val 
            100                 105                 110         
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys 
        115                 120                 125             
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala 
    130                 135                 140                 
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys 
145                 150                 155                 160 
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser 
                165                 170                 175     
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp 
            180                 185                 190         
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg 
        195                 200                 205             
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp 
    210                 215                 220                 
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg 
225                 230                 235                 240 
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu 
                245                 250                 255     
Val Ile Gly Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met 
            260                 265                 270         
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu 
        275                 280                 285             
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val 
    290                 295                 300                 
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg 
305                 310                 315                 320 
Gln Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala 
                325                 330                 335     
Trp Ala Val Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser 
            340                 345                 350         
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro 
        355                 360                 365             
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe 
    370                 375                 380                 
Tyr Glu Trp Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr 
385                 390                 395                 400 
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu 
                405                 410                 415     
Leu                                                             
                                                                

 
<210> 493
<211> 1251
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 493
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctccaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt catggagatg 180
gcagagctga tggtcactga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat tatgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaaaagtat aaagagtatc ttggactgga catcttttaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctggaatca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgagcc atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
cagggagaca actttgaagt gtgggaacga cctctctcag gcttagcctg ggctgtagct 1020
atgataaacc ggcaggagat tggtggacct cgctcttata ccatcgcagt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaatg gacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t          1251

 
<210> 494
<211> 417
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 494
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp 
            20                  25                  30          
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro 
        35                  40                  45              
Asp Ser Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met 
    50                  55                  60                  
Val Thr Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp 
65                  70                  75                  80  
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala 
                85                  90                  95      
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val 
            100                 105                 110         
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys 
        115                 120                 125             
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala 
    130                 135                 140                 
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys 
145                 150                 155                 160 
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser 
                165                 170                 175     
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp 
            180                 185                 190         
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg 
        195                 200                 205             
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp 
    210                 215                 220                 
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg 
225                 230                 235                 240 
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu 
                245                 250                 255     
Val Ile Gly Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met 
            260                 265                 270         
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu 
        275                 280                 285             
Arg Ala Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val 
    290                 295                 300                 
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg 
305                 310                 315                 320 
Gln Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala 
                325                 330                 335     
Trp Ala Val Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser 
            340                 345                 350         
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro 
        355                 360                 365             
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe 
    370                 375                 380                 
Tyr Glu Trp Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr 
385                 390                 395                 400 
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu 
                405                 410                 415     
Leu                                                             
                                                                

 
<210> 495
<211> 1251
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 495
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctccaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt catggagatg 180
gcagagctca tggtctcaga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat tatgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaagagtat aaagagtatc ttggactgga catcttttaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctggaacca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
cagggagaca actttgaagt gtgggaacga cctctctcag gcttagcctg ggctgtagct 1020
atgataaacc ggcaggagat tggtggacct cgctcttata ccatcgcagt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaagc tacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t          1251

 
<210> 496
<211> 417
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 496
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp 
            20                  25                  30          
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro 
        35                  40                  45              
Asp Ser Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met 
    50                  55                  60                  
Val Ser Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp 
65                  70                  75                  80  
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala 
                85                  90                  95      
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val 
            100                 105                 110         
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys 
        115                 120                 125             
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala 
    130                 135                 140                 
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys 
145                 150                 155                 160 
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser 
                165                 170                 175     
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp 
            180                 185                 190         
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg 
        195                 200                 205             
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp 
    210                 215                 220                 
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg 
225                 230                 235                 240 
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu 
                245                 250                 255     
Val Ile Gly Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met 
            260                 265                 270         
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu 
        275                 280                 285             
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val 
    290                 295                 300                 
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg 
305                 310                 315                 320 
Gln Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala 
                325                 330                 335     
Trp Ala Val Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser 
            340                 345                 350         
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro 
        355                 360                 365             
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe 
    370                 375                 380                 
Tyr Glu Ala Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr 
385                 390                 395                 400 
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu 
                405                 410                 415     
Leu                                                             
                                                                

 
<210> 497
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 497
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaagag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 498
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 498
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 499
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 499
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatctag gaaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
ggtcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcctcgc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatccc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 500
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 500
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 501
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 501
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctgggccag tataaagagt atcttggact ggacatctag gaaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 502
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 502
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 503
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 503
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctgggccag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctatgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagacctcat taaaagactt actt       1194

 
<210> 504
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 504
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 505
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 505
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctgggccag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcctcgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga agctacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 506
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 506
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 507
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 507
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga agctacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 508
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 508
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 509
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 509
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaagag tataaagagt atcttggact ggacatctag gaaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 510
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 510
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 511
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 511
ctggacaatg gattggcaag gacgcctacc atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatccc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 512
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 512
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 513
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 513
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaagag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 514
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 514
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 515
<211> 1251
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 515
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctacaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt catggagatg 180
gcagagctca tggtctcaga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat catgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaagagtat aaagagtatc ttggactgga catcttttaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctgggacca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
cagggagaca actttgaagt gtgggaacga cctctctcag gcttagcctg ggctgtagct 1020
atgataaacc ggcaggagat tggtggacct cgctcttata ccatcgccgt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaatg gacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t          1251

 
<210> 516
<211> 417
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 516
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp 
            20                  25                  30          
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro 
        35                  40                  45              
Asp Ser Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met 
    50                  55                  60                  
Val Ser Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp 
65                  70                  75                  80  
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala 
                85                  90                  95      
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val 
            100                 105                 110         
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys 
        115                 120                 125             
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala 
    130                 135                 140                 
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys 
145                 150                 155                 160 
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser 
                165                 170                 175     
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp 
            180                 185                 190         
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg 
        195                 200                 205             
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp 
    210                 215                 220                 
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg 
225                 230                 235                 240 
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu 
                245                 250                 255     
Val Ile Gly Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met 
            260                 265                 270         
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu 
        275                 280                 285             
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val 
    290                 295                 300                 
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg 
305                 310                 315                 320 
Gln Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala 
                325                 330                 335     
Trp Ala Val Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser 
            340                 345                 350         
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro 
        355                 360                 365             
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe 
    370                 375                 380                 
Tyr Glu Trp Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr 
385                 390                 395                 400 
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu 
                405                 410                 415     
Leu                                                             
                                                                

 
<210> 517
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 517
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcctcgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 518
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 518
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 519
<211> 1251
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 519
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctacaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt catggagatg 180
gcagagctca tggtctcaga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat catgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaaaagtat aaagagtatc ttggactgga catcttttaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctgggacca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
aagggagaca actttgaagt gtgggaacga cctctctcag gcttagcctg ggctgtagct 1020
attataaacc ggcaggagat tggtggacct cgctcttata ccatccccgt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaatg gacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t          1251

 
<210> 520
<211> 417
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 520
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp 
            20                  25                  30          
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro 
        35                  40                  45              
Asp Ser Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met 
    50                  55                  60                  
Val Ser Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp 
65                  70                  75                  80  
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala 
                85                  90                  95      
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val 
            100                 105                 110         
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys 
        115                 120                 125             
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala 
    130                 135                 140                 
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys 
145                 150                 155                 160 
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser 
                165                 170                 175     
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp 
            180                 185                 190         
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg 
        195                 200                 205             
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp 
    210                 215                 220                 
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg 
225                 230                 235                 240 
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu 
                245                 250                 255     
Val Ile Gly Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met 
            260                 265                 270         
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu 
        275                 280                 285             
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val 
    290                 295                 300                 
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg 
305                 310                 315                 320 
Lys Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala 
                325                 330                 335     
Trp Ala Val Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser 
            340                 345                 350         
Tyr Thr Ile Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro 
        355                 360                 365             
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe 
    370                 375                 380                 
Tyr Glu Trp Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr 
385                 390                 395                 400 
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu 
                405                 410                 415     
Leu                                                             
                                                                

 
<210> 521
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 521
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcctcgc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 522
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 522
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 523
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 523
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 524
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 524
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 525
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 525
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 526
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 526
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 527
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 527
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg       1194

 
<210> 528
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 528
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 529
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 529
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaagtc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 530
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 530
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 531
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 531
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 532
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 532
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 533
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 533
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat gggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
caaaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg       1194

 
<210> 534
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 534
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Gly Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 535
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 535
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga atggaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 536
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 536
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 537
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 537
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg       1194

 
<210> 538
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 538
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 539
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 539
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 540
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 540
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 541
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 541
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaagtc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg       1194

 
<210> 542
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 542
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 543
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 543
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 544
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 544
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 545
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 545
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 546
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 546
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 547
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 547
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agacagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg       1194

 
<210> 548
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 548
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 549
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 549
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agacagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 550
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 550
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 551
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 551
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 552
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 552
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 553
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 553
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaagtc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 554
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 554
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 555
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 555
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agacagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg       1194

 
<210> 556
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 556
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 557
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 557
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agacagggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 558
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 558
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 559
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 559
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga atggaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 560
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 560
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 561
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 561
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg       1194

 
<210> 562
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 562
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 563
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 563
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 564
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 564
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 565
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 565
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 566
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 566
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 567
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 567
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaagtc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg       1194

 
<210> 568
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 568
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 569
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 569
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga atggaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 570
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 570
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 571
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 571
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaagtc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcgccaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 572
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 572
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 573
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 573
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaagtc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 574
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 574
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 575
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 575
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agacagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 576
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 576
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 577
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 577
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 578
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 578
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 579
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 579
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 580
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 580
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 581
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 581
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 582
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 582
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 583
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 583
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 584
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 584
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 585
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 585
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaaaatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agacagggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg       1194

 
<210> 586
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 586
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asn Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 587
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 587
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaagtc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 588
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 588
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 589
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 589
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 590
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 590
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 591
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 591
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagatgct ctttgaagaa 120
atggccgaac ggatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 592
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 592
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Met Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 593
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 593
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac ggatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg       1194

 
<210> 594
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 594
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 595
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 595
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga atggaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 596
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 596
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 597
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 597
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg       1194

 
<210> 598
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 598
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 599
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 599
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 600
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 600
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 601
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 601
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 602
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 602
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 603
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 603
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaagtc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga atggaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 604
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 604
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 605
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 605
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaagtc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 606
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 606
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 607
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 607
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 608
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 608
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 609
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 609
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 610
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 610
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 611
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 611
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaagtc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 612
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 612
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 613
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 613
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 614
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 614
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 615
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 615
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agacagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 616
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 616
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 617
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 617
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agacagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 618
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 618
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 619
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 619
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 620
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 620
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 621
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 621
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaagtc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 622
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 622
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 623
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 623
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaagtc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 624
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 624
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 625
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 625
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 626
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 626
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 627
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 627
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 628
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 628
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 629
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 629
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 630
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 630
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 631
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 631
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 632
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 632
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 633
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 633
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 634
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 634
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 635
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 635
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga atggaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 636
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 636
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 637
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 637
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 638
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 638
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 639
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 639
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 640
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 640
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 641
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 641
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga atggaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 642
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 642
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 643
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 643
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac ggatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg       1194

 
<210> 644
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 644
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 645
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 645
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 646
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 646
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 647
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 647
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 648
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 648
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 649
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 649
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 650
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 650
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 651
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 651
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 652
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 652
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 653
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 653
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga atggaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg       1194

 
<210> 654
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 654
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 655
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 655
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg       1194

 
<210> 656
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 656
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 657
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 657
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 658
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 658
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 659
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 659
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 660
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 660
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 661
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 661
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg       1194

 
<210> 662
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 662
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 663
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 663
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaaacaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg       1194

 
<210> 664
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 664
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 665
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 665
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 666
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 666
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 667
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 667
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 668
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 668
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 669
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 669
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaagtc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg       1194

 
<210> 670
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 670
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 671
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 671
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg       1194

 
<210> 672
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 672
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 673
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 673
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 674
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 674
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 675
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 675
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 676
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 676
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 677
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 677
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 678
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 678
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 679
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 679
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 680
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 680
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 681
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 681
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 682
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 682
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 683
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 683
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agacagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 684
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 684
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 685
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 685
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 686
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 686
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 687
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 687
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 688
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 688
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 689
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 689
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agacagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 690
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 690
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 691
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 691
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 692
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 692
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 693
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 693
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg       1194

 
<210> 694
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 694
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 695
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 695
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 696
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 696
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 697
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 697
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 698
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 698
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 699
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 699
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg       1194

 
<210> 700
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 700
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 701
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 701
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac ggatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 702
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 702
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

<210> 703
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 703
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 704
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 704
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 705
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 705
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggac gtcttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaaccaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatga ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
cgtaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgttctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cttt       1194

 
<210> 706
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 706
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Thr Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Phe         
385                 390                 395             

 
<210> 707
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 707
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc aacatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 708
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 708
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Asn Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 709
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 709
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgcatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc aacatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcgggt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcaatatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga aaatacgatg aacacttcac tgaaggacct cctg       1194

 
<210> 710
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 710
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Asn Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Asn 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Asn Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 711
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 711
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc aacatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcaatt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 712
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 712
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Asn Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Asn Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 713
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 713
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc aacatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcaatt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgctttatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga aaatacgaat aacacttcac tgaaggacct cctg       1194

 
<210> 714
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 714
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Asn Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Asn Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Asn Asn Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 715
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 715
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc aacatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcaatt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg aacacttcac tgaaggacct cctg       1194

 
<210> 716
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 716
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Asn Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Asn Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Asn Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 717
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 717
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa gaatctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 718
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 718
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Asn Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 719
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 719
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa gaggctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 720
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 720
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Arg Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 721
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 721
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa gagtctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 722
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 722
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ser Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 723
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 723
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatacg tatcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 724
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 724
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Arg Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 725
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 725
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtaggttct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 726
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 726
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Gly Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 727
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 727
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtaaattct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 728
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 728
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Asn Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 729
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 729
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtacagtct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 730
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 730
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Gln Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 731
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 731
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagtttct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 732
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 732
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Val Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 733
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 733
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgcatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctacagtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 734
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 734
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 735
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 735
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctacagtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc aacatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcgggt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga aaatacgatg caaacttcac tgaaggacct cctg       1194

 
<210> 736
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 736
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Asn Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 737
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 737
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctacagtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc aacatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcgggt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcaatatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga aaatacgaat caaacttcac tgaaggacct cctg       1194

 
<210> 738
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 738
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Asn Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Asn 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Asn Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 739
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 739
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctacagtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcaatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 740
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 740
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asn Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 741
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 741
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctacagtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcgggt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgctttatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgaa taatacgatg aacacttcac tgaaggacct cctg       1194

 
<210> 742
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 742
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Asn Asn Thr Met Asn Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 743
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 743
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctacagtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcaatt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 744
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 744
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Asn Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 745
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 745
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgcatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctacagtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcaatt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgctttatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgaa taatacgact caaacttcac tgaaggacct cctg       1194

 
<210> 746
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 746
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Asn Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Asn Asn Thr Thr Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 747
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 747
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctggctatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcgggt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 748
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 748
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Ala Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 749
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 749
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctggctatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctacagtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcgggt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 750
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 750
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Ala Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 751
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 751
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctggctatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctacagtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc aacatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcgggt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgctttatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 752
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 752
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Ala Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Asn Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 753
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 753
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctggctatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcaatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcgggt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 754
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 754
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Ala Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asn Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 755
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 755
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgactatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgctttatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 756
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 756
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Thr Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 757
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 757
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgactatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctacagtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcaatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcgccaaga gatcggaggt ccacgcgggt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 758
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 758
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Thr Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asn Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 759
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 759
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgactatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctacagtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcaatt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 760
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 760
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Thr Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Asn Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 761
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 761
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgactatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcaatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcaatt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgctttatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 762
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 762
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Thr Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asn Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Asn Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 763
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 763
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctggtaatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc aacatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgctttatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgaa taatacgact caaacttcac tgaaggacct cctg       1194

 
<210> 764
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 764
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Val Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Asn Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Asn Asn Thr Thr Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 765
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 765
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctggtaatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctacagtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcaatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc aacatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcaatt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgctttatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 766
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 766
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Val Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asn Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Asn Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Asn Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 767
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 767
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgagattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 768
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 768
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Glu Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 769
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 769
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tataatattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 770
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 770
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asn Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 771
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 771
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tattcgattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 772
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 772
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Ser Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 773
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 773
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgcatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcaatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcgggt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgctttatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 774
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 774
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asn Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 775
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 775
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcaatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcaatt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgctttatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga aaatacgatg caaacttcac tgaaggacct cctg       1194

 
<210> 776
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 776
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asn Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Asn Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 777
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 777
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgt cgtgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc aacatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcgggt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcaatatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 778
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 778
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Ser Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Asn Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Asn 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 779
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 779
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgt cgtgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctacagta acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 780
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 780
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Ser Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asn 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 781
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 781
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgt cgtgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctacagtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcaatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcgggt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcaatatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga aaatacgaat aacacttcac tgaaggacct cctg       1194

 
<210> 782
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 782
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Ser Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asn Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Asn 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Asn Asn Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 783
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 783
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgt cgtgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgcatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctacagtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcaatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcaatt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgctttatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 784
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 784
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Ser Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asn Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Asn Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 785
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 785
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgt cgtgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctacagtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcaatt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcaatatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgaa taatacgact aacacttcac tgaaggacct cctg       1194

 
<210> 786
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 786
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Ser Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Asn Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Asn 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Asn Asn Thr Thr Asn Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 787
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 787
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgt cgtgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctacagtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga aaatacgact aacacttcac tgaaggacct cctg       1194

 
<210> 788
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 788
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Ser Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Thr Asn Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 789
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 789
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgt cgtgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctggctatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcgggt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgctttatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga aaatacgatg caaacttcac tgaaggacct cctg       1194

 
<210> 790
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 790
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Ser Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Ala Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 791
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 791
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgt cgtgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcaatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 792
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 792
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Ser Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asn Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 793
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 793
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgt cgtgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcaatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc aacatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcgggt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgctttatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 794
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 794
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Ser Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asn Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Asn Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 795
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 795
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgt cgtgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcaatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcaatt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcaatatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 796
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 796
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Ser Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asn Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Asn Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Asn 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 797
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 797
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgt cgtgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgaa taatacgatg aacacttcac tgaaggacct cctg       1194

 
<210> 798
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 798
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Ser Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Asn Asn Thr Met Asn Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 799
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 799
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgt cgtgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcgggt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcaatatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgaa taatacgaat aacacttcac tgaaggacct cctg       1194

 
<210> 800
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 800
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Ser Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Asn 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Asn Asn Thr Asn Asn Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 801
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 801
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag cggtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 802
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 802
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Ala Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 803
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 803
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag aggtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 804
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 804
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Glu Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 805
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 805
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag gtgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 806
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 806
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Gly Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 807
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 807
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaac ttgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 808
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 808
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Leu Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 809
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 809
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaaa tggtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 810
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 810
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Met Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 811
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 811
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaaa gggtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 812
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 812
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Arg Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 813
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 813
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaat cggtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 814
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 814
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Ser Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 815
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 815
ctgagtaatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 816
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 816
Leu Ser Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 817
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 817
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggca ctaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 818
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 818
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Thr 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 819
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 819
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg tgaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 820
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 820
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Val 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 821
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 821
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggct ggaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 822
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 822
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Trp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 823
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 823
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcct taacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 824
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 824
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Leu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 825
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 825
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcag taacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 826
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 826
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Ser Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 827
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 827
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacgc tgctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 828
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 828
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Ala Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 829
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 829
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga tgctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 830
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 830
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Asp Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 831
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 831
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacct ggctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 832
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 832
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Leu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 833
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 833
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacat ggctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 834
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 834
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Met Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 835
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 835
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgcatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcgggt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgaa taatacgatg aacacttcac tgaaggacct cctg       1194

 
<210> 836
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 836
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Asn Asn Thr Met Asn Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 837
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 837
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgagcag 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 838
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 838
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Gln Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 839
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 839
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg agcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 840
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 840
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Glu Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 841
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 841
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaagctg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 842
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 842
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Ala Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 843
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 843
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaatgtg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 844
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 844
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Cys Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 845
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 845
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaatggg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 846
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 846
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Trp Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 847
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 847
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcaatt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcaatatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 848
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 848
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Asn Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Asn 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 849
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 849
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcaatt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga aaatacgaat aacacttcac tgaaggacct cctg       1194

 
<210> 850
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 850
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Asn Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Asn Asn Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 851
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 851
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcaatt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgctttatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga aaatacgagt aacacttcac tgaaggacct cctg       1194

 
<210> 852
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 852
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Asn Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Ser Asn Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 853
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 853
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgcatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcaatt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgctttatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga aaatacgatg aacacttcac tgaaggacct cctg       1194

 
<210> 854
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 854
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Asn Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Asn Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 855
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 855
ctggataatt tgttggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 856
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 856
Leu Asp Asn Leu Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 857
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 857
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgaggcta gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 858
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 858
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Ala Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 859
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 859
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca aggctatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 860
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 860
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys Ala Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 861
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 861
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca aggatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 862
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 862
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys Asp Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 863
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 863
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agtttatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 864
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 864
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys Phe Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 865
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 865
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agttgatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 866
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 866
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys Leu Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 867
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 867
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcgtatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 868
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 868
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys Arg Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 869
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 869
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agactatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 870
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 870
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys Thr Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 871
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 871
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat ccttgatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 872
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 872
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser Leu Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 873
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 873
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccagatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 874
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 874
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser Gln Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 875
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 875
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctg ggggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 876
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 876
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro Gly Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 877
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 877
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttcccta agggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 878
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 878
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro Lys Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 879
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 879
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc ggggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 880
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 880
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro Arg Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 881
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 881
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgaccagg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 882
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 882
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Gln Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 883
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 883
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacaggg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 884
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 884
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Arg Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 885
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 885
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgactcgg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 886
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 886
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ser Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 887
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 887
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacacgg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 888
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 888
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Thr Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 889
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 889
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacatttcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 890
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 890
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Phe 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 891
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 891
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaggtcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 892
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 892
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Gly 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 893
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 893
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaagtcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 894
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 894
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ser 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 895
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 895
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaacgcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 896
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 896
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Thr 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 897
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 897
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaca ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 898
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 898
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Gln Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 899
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 899
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcagt ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 900
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 900
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Val Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 901
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 901
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagaccttg atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 902
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 902
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Leu Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 903
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 903
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagaccctg atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 904
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 904
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Pro Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 905
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 905
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacactg atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 906
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 906
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Thr Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 907
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 907
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacgtgg atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 908
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 908
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Val Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 909
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 909
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggccttg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 910
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 910
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Leu Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 911
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 911
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggccggg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 912
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 912
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Arg Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 913
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 913
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcagtg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 914
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 914
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Ser Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 915
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 915
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggctggg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 916
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 916
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Trp Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 917
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 917
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgcat 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 918
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 918
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val His Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 919
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 919
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtggtt 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 920
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 920
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Val Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 921
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 921
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agacatctgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 922
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 922
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg His Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 923
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 923
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgggt 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 924
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 924
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Gly Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 925
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 925
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagttt 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgttctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 926
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 926
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Phe Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 927
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 927
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
tttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 928
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 928
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Phe Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 929
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 929
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
atgggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 930
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 930
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Met Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 931
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 931
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cttt       1194

 
<210> 932
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 932
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Phe         
385                 390                 395             

 
<210> 933
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 933
ctggataatg gaatggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 934
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 934
Leu Asp Asn Gly Met Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 935
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 935
ctggataatg gagtggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 936
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 936
Leu Asp Asn Gly Val Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 937
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 937
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgcatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga aaatacgagt caaacttcac tgaaggacct cctg       1194

 
<210> 938
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 938
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Ser Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 939
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 939
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcatacg ccagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggactagtcg gtaccaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
cgtaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 940
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 940
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Tyr Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 941
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 941
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggac gtcttcaggc tgatccgcag 240
cgcttccctc atggcatacg ccagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggactagtcg gtaccaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgttctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cttt       1194

 
<210> 942
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 942
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Tyr Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Phe         
385                 390                 395             

 
<210> 943
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 943
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggac gtcttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggactagtcg gtaccaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
accaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 944
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 944
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Tyr Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Thr Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 945
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 945
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggac gtcttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggactagtcg gtaccaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
accaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cttt       1194

 
<210> 946
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 946
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Tyr Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Thr Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Phe         
385                 390                 395             

 
<210> 947
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 947
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcca gcctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 948
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 948
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Gln Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 949
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 949
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcatacg ccagctggca agccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggagtagtcg gaaccaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
accaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgttctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 950
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 950
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Ser His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Ser Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Thr Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 951
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 951
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggcatttt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 952
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 952
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp His Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 953
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 953
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggctgttt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 954
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 954
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Leu Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 955
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 955
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggaggttt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 956
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 956
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Arg Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 957
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 957
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggtggttt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 958
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 958
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Trp Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 959
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 959
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt atgcgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 960
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 960
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Met Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 961
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 961
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccggc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 962
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 962
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Arg His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 963
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 963
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttctctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 964
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 964
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Ser His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 965
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 965
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cagcggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 966
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 966
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Ala Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 967
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 967
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac caggtgcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 968
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 968
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gly Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 969
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 969
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
attgacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 970
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 970
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Ile Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 971
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 971
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
atggacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 972
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 972
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Met Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 973
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 973
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgaggctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 974
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 974
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Arg Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 975
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 975
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttgcggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 976
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 976
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Ala Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 977
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 977
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gactttttgc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 978
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 978
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Phe Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 979
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 979
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttatggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 980
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 980
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Met Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 981
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 981
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gactttcggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 982
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 982
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Ser Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 983
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 983
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgacg 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 984
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 984
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Thr 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 985
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 985
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtattccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 986
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 986
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Ile Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 987
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 987
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atgcgcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 988
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 988
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Ala Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 989
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 989
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccagggggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 990
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 990
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Gly Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 991
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 991
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacatggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 992
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 992
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro His Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 993
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 993
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
accaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 994
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 994
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Thr Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 995
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 995
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
gttaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 996
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 996
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Val Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 997
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 997
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc gggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 998
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 998
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Gly Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 999
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 999
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctcttgt cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1000
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1000
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Leu Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1001
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1001
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctctctt cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1002
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1002
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Ser Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1003
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1003
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagatt atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1004
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1004
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ile Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1005
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1005
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatccttc cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1006
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1006
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Leu Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1007
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1007
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac ttttgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1008
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1008
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Phe Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1009
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1009
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac cattgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1010
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1010
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp His Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1011
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1011
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac ctgtgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1012
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1012
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Leu Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1013
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1013
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac acgtgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1014
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1014
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Thr Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1015
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1015
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac tggtgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1016
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1016
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Trp Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1017
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1017
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcacat 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1018
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1018
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala His Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1019
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1019
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaatt 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1020
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1020
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ile Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1021
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1021
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1022
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1022
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1023
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1023
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaatg 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1024
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1024
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Met Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1025
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1025
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcacct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1026
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1026
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Pro Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1027
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1027
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatgg 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1028
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1028
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Trp Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1029
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1029
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacgagga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1030
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1030
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Glu Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1031
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1031
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actactttga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1032
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1032
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Phe Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1033
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1033
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacatgga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1034
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1034
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Met Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1035
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1035
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actaccctga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1036
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1036
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Pro Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1037
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1037
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacaggga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1038
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1038
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Arg Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1039
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1039
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actactcgga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1040
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1040
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Ser Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1041
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1041
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actactatga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1042
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1042
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Tyr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1043
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1043
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggagtagtcg gtaccaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1044
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1044
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Ser Ser Arg Tyr Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1045
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1045
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atgcgattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1046
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1046
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Ala Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1047
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1047
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atctgattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1048
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1048
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Leu Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1049
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1049
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctgatagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1050
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1050
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Asp Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1051
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1051
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tcttgcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1052
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1052
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Leu Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1053
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1053
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccgtttaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1054
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1054
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Phe Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1055
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1055
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccgctgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1056
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1056
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Leu Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1057
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1057
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccgcgtaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1058
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1058
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Arg Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1059
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1059
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accattctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1060
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1060
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Ile Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1061
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1061
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accattctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cttt       1194

 
<210> 1062
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1062
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Ile Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Phe         
385                 390                 395             

 
<210> 1063
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1063
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttatga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1064
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1064
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Tyr Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1065
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1065
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt gtacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1066
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1066
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Cys Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1067
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1067
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtg tgacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1068
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1068
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Val Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1069
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1069
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggtgttc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1070
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1070
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Cys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1071
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1071
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggattc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1072
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1072
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Asp Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1073
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1073
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggaatc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1074
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1074
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Glu Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1075
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1075
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggttttc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1076
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1076
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Phe Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1077
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1077
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggggtc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1078
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1078
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Gly Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1079
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1079
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggcattc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1080
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1080
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp His Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1081
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1081
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggatctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1082
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1082
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ile Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1083
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1083
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaaatc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1084
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1084
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1085
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1085
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggctgtc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1086
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1086
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Leu Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1087
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1087
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggatgtc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1088
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1088
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Met Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1089
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1089
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaattc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1090
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1090
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Asn Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1091
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1091
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggccatc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1092
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1092
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Pro Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1093
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1093
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggcaatc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1094
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1094
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Gln Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1095
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1095
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggcgttc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1096
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1096
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Arg Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1097
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1097
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggagttc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1098
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1098
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ser Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1099
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1099
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggacttc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1100
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1100
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Thr Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1101
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1101
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggtctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1102
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1102
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Val Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1103
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1103
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggtggtc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1104
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1104
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Trp Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1105
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1105
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggtattc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1106
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1106
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Tyr Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1107
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1107
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc tgtatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1108
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1108
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Cys Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1109
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1109
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gatatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1110
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1110
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Asp Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1111
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1111
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gagatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1112
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1112
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Glu Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1113
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1113
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc tttatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1114
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1114
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Phe Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1115
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1115
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc ggtatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1116
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1116
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Gly Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1117
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1117
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc cacatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1118
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1118
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1119
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1119
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc attatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1120
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1120
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ile Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1121
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1121
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc aagatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1122
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1122
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Lys Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1123
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1123
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc ttgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1124
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1124
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Leu Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1125
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1125
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc atgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1126
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1126
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Met Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1127
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1127
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc aatatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1128
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1128
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Asn Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1129
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1129
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc cctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1130
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1130
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Pro Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1131
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1131
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc cagatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1132
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1132
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Gln Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1133
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1133
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc cggatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1134
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1134
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Arg Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1135
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1135
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc tcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1136
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1136
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ser Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1137
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1137
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc actatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1138
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1138
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Thr Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1139
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1139
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gttatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1140
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1140
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Val Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1141
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1141
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc tggatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1142
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1142
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Trp Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1143
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1143
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc tacatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1144
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1144
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Tyr Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1145
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1145
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga atgtaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1146
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1146
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Cys 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1147
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1147
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agataccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1148
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1148
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Asp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1149
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1149
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agaaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1150
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1150
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Glu 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1151
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1151
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga atttaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1152
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1152
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Phe 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1153
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1153
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga aggtaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1154
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1154
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Gly 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1155
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1155
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga acataccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1156
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1156
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu His 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1157
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1157
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga aattaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1158
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1158
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ile 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1159
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1159
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga aaagaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1160
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1160
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Lys 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1161
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1161
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga acttaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1162
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1162
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Leu 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1163
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1163
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga aatgaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1164
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1164
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Met 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1165
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1165
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga aaataccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1166
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1166
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Asn 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1167
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1167
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga acctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1168
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1168
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Pro 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1169
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1169
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga acaaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1170
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1170
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Gln 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1171
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1171
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga acggaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1172
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1172
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Arg 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1173
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1173
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga aagtaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1174
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1174
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ser 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1175
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1175
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga aacgaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1176
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1176
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Thr 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1177
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1177
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agtgaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1178
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1178
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Val 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1179
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1179
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga atggaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1180
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1180
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1181
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1181
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga atataccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1182
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1182
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Tyr 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1183
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1183
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttgggc tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1184
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1184
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Ala Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1185
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1185
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggtg tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1186
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1186
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Cys Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1187
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1187
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga gcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1188
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1188
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Glu Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1189
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1189
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggtt tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1190
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1190
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Phe Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1191
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1191
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttgggg gcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1192
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1192
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Gly Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1193
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1193
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggca tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1194
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1194
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp His Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1195
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1195
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggat tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1196
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1196
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Ile Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1197
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1197
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa gcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1198
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1198
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Lys Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1199
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1199
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggtt gcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1200
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1200
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Leu Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1201
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1201
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggat gcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1202
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1202
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Met Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1203
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1203
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1204
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1204
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1205
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1205
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggcc gcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1206
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1206
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Pro Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1207
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1207
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggca gcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1208
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1208
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Gln Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1209
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1209
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggcg gcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1210
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1210
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Arg Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1211
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1211
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggtc tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1212
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1212
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Ser Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1213
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1213
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggac gcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1214
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1214
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Thr Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1215
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1215
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttgggt gcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1216
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1216
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Val Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1217
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1217
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggtg gcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1218
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1218
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Trp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1219
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1219
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggta tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1220
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1220
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Tyr Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1221
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1221
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagcggc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1222
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1222
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Ala Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1223
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1223
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggatgtgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1224
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1224
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Cys Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1225
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1225
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagaggc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1226
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1226
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Glu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1227
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1227
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggatttgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1228
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1228
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Phe Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1229
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1229
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggaggggc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1230
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1230
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Gly Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1231
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1231
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggacatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1232
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1232
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly His Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1233
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1233
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggaattgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1234
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1234
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Ile Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1235
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1235
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggaaaagc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1236
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1236
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Lys Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1237
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1237
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggacttgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1238
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1238
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1239
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1239
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggaatggc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1240
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1240
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Met Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1241
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1241
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggaaacgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1242
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1242
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asn Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1243
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1243
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggacctgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1244
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1244
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Pro Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1245
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1245
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggacaggc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1246
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1246
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Gln Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1247
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1247
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggacgggc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1248
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1248
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Arg Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1249
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1249
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggatcggc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1250
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1250
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Ser Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1251
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1251
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggaacggc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1252
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1252
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Thr Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1253
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1253
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagtggc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1254
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1254
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Val Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1255
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1255
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggatgggc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1256
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1256
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Trp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1257
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1257
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggatatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1258
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1258
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Tyr Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1259
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1259
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgcggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1260
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1260
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Ala Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1261
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1261
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct cttttgtgaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1262
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1262
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Cys Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1263
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1263
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgatgaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg       1194

 
<210> 1264
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1264
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Asp Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1265
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1265
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttttcgaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1266
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1266
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Phe Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1267
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1267
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttggtgaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1268
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1268
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Gly Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1269
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1269
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttcatgaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1270
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1270
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe His Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1271
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1271
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttattgaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1272
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1272
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Ile Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1273
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1273
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttaaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1274
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1274
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Lys Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1275
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1275
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttctggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1276
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1276
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Leu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1277
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1277
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1278
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1278
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1279
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1279
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttaatgaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1280
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1280
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Asn Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1281
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1281
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttccggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1282
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1282
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Pro Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1283
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1283
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttcaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1284
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1284
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Gln Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1285
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1285
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttagggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1286
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1286
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Arg Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1287
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1287
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttagcgaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1288
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1288
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Ser Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1289
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1289
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttacggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1290
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1290
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Thr Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1291
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1291
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgtggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1292
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1292
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Val Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1293
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1293
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct cttttgggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1294
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1294
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Trp Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1295
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1295
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct cttttatgaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1296
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1296
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Tyr Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1297
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1297
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcgcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1298
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1298
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1299
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1299
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
tgtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1300
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1300
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Cys Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1301
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1301
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gatcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1302
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1302
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Asp Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1303
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1303
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gagcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1304
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1304
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Glu Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1305
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1305
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
tttcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1306
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1306
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Phe Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1307
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1307
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
caccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1308
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1308
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala His Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1309
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1309
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
attcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1310
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1310
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ile Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1311
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1311
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
aagcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1312
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1312
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Lys Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1313
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1313
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ttgcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1314
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1314
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Leu Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1315
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1315
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
atgcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1316
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1316
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Met Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1317
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1317
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
aatcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1318
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1318
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Asn Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1319
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1319
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ccgcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1320
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1320
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Pro Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1321
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1321
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
cagcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1322
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1322
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gln Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1323
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1323
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
aggcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1324
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1324
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Arg Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1325
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1325
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
tcgcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1326
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1326
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ser Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1327
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1327
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
acgcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1328
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1328
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Thr Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1329
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1329
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gtgcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1330
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1330
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Val Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1331
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1331
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
tggcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1332
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1332
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Trp Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1333
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1333
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
tatcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1334
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1334
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Tyr Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1335
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1335
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacgctgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1336
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1336
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Ala Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1337
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1337
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactgtgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1338
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1338
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Cys Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1339
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1339
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacgatgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1340
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1340
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Asp Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1341
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1341
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacgaggtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1342
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1342
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Glu Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1343
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1343
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactttgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1344
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1344
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Phe Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1345
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1345
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacggtgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1346
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1346
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Gly Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1347
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1347
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacatcgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1348
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1348
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Ile Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1349
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1349
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacaaggtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1350
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1350
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Lys Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1351
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1351
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacttggtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1352
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1352
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Leu Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1353
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1353
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacatggtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1354
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1354
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Met Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1355
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1355
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacaatgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1356
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1356
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Asn Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1357
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1357
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacccggtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1358
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1358
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Pro Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1359
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1359
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccaggtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1360
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1360
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Gln Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1361
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1361
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccgtgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1362
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1362
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Arg Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1363
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1363
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactcggtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1364
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1364
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Ser Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1365
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1365
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacactgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1366
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1366
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Thr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1367
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1367
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacgttgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1368
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1368
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Val Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1369
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1369
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactgggtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1370
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1370
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Trp Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1371
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1371
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1372
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1372
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1373
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1373
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccgcgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1374
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1374
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ala Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1375
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1375
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gcctgtatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1376
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1376
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Cys Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1377
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1377
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccgatatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1378
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1378
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Asp Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1379
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1379
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccgagatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1380
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1380
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Glu Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1381
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1381
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gcctttatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1382
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1382
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Phe Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1383
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1383
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccggtatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1384
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1384
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Gly Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1385
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1385
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gcccatatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1386
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1386
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala His Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1387
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1387
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccaagatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1388
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1388
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Lys Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1389
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1389
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gcccttatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1390
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1390
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Leu Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1391
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1391
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1392
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1392
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1393
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1393
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccaatatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1394
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1394
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Asn Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1395
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1395
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gcccctatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1396
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1396
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Pro Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1397
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1397
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gcccagatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1398
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1398
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Gln Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1399
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1399
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gcccggatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1400
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1400
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Arg Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1401
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1401
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gcctctatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1402
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1402
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ser Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1403
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1403
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccacgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1404
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1404
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Thr Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1405
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1405
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccgttatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1406
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1406
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Val Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1407
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1407
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gcctggatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1408
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1408
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Trp Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1409
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1409
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gcctatatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1410
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1410
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Tyr Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1411
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1411
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agagcgggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1412
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1412
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Ala Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1413
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1413
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agatgcggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1414
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1414
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Cys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1415
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1415
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agagatggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1416
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1416
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Asp Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1417
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1417
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agagagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1418
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1418
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Glu Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1419
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1419
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agatttggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1420
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1420
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Phe Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1421
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1421
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaggtggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1422
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1422
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gly Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1423
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1423
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agacatggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1424
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1424
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg His Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1425
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1425
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaattggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1426
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1426
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Ile Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1427
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1427
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agactgggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1428
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1428
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Leu Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1429
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1429
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaatgggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1430
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1430
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Met Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1431
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1431
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaatggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1432
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1432
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Asn Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1433
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1433
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaccgggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1434
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1434
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Pro Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1435
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1435
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agacagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1436
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1436
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1437
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1437
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaggggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1438
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1438
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Arg Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1439
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1439
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agatctggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1440
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1440
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Ser Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1441
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1441
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaactggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1442
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1442
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Thr Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1443
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1443
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agagtgggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1444
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1444
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Val Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1445
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1445
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agatggggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1446
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1446
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Trp Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1447
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1447
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agatatggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1448
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1448
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Tyr Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1449
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1449
ctggataatg gattggctag aacacctgct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1450
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1450
Leu Asp Asn Gly Leu Ala Arg Thr Pro Ala Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1451
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1451
ctggataatg gattggctag aacaccttgt atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1452
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1452
Leu Asp Asn Gly Leu Ala Arg Thr Pro Cys Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1453
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1453
ctggataatg gattggctag aacacctgat atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcatc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1454
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1454
Leu Asp Asn Gly Leu Ala Arg Thr Pro Asp Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1455
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1455
ctggataatg gattggctag aacacctgag atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1456
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1456
Leu Asp Asn Gly Leu Ala Arg Thr Pro Glu Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1457
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1457
ctggataatg gattggctag aacacctttt atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1458
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1458
Leu Asp Asn Gly Leu Ala Arg Thr Pro Phe Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1459
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1459
ctggataatg gattggctag aacacctggc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agccaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1460
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1460
Leu Asp Asn Gly Leu Ala Arg Thr Pro Gly Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1461
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1461
ctggataatg gattggctag aacacctcat atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1462
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1462
Leu Asp Asn Gly Leu Ala Arg Thr Pro His Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1463
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1463
ctggataatg gattggctag aacacctatt atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1464
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1464
Leu Asp Asn Gly Leu Ala Arg Thr Pro Ile Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1465
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1465
ctggataatg gattggctag aacacctaag atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1466
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1466
Leu Asp Asn Gly Leu Ala Arg Thr Pro Lys Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1467
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1467
ctggataatg gattggctag aacacctttg atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1468
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1468
Leu Asp Asn Gly Leu Ala Arg Thr Pro Leu Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1469
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1469
ctggataatg gattggctag aacacctatg atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1470
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1470
Leu Asp Asn Gly Leu Ala Arg Thr Pro Met Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1471
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1471
ctggataatg gattggctag aacacctaat atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1472
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1472
Leu Asp Asn Gly Leu Ala Arg Thr Pro Asn Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1473
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1473
ctggataatg gattggctag aacacctcag atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1474
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1474
Leu Asp Asn Gly Leu Ala Arg Thr Pro Gln Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1475
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1475
ctggataatg gattggctag aacacctcgt atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1476
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1476
Leu Asp Asn Gly Leu Ala Arg Thr Pro Arg Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1477
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1477
ctggataatg gattggctag aacaccttct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1478
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1478
Leu Asp Asn Gly Leu Ala Arg Thr Pro Ser Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1479
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1479
ctggataatg gattggctag aacacctact atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1480
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1480
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1481
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1481
ctggataatg gattggctag aacacctgtc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1482
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1482
Leu Asp Asn Gly Leu Ala Arg Thr Pro Val Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1483
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1483
ctggataatg gattggctag aacaccttgg atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1484
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1484
Leu Asp Asn Gly Leu Ala Arg Thr Pro Trp Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1485
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1485
ctggataatg gattggctag aacaccttat atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1486
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1486
Leu Asp Asn Gly Leu Ala Arg Thr Pro Tyr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1487
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1487
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcgggcgat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1488
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1488
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ala Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1489
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1489
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtgcat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1490
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1490
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Cys Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1491
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1491
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcgggatat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1492
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1492
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Asp Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1493
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1493
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcgggagat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1494
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1494
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Glu Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1495
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1495
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtttat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1496
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1496
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Phe Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1497
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1497
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcgggggat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1498
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1498
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Gly Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1499
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1499
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggcatat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1500
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1500
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg His Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1501
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1501
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggattat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1502
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1502
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ile Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1503
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1503
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggaagat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1504
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1504
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Lys Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1505
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1505
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggctgat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1506
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1506
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Leu Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1507
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1507
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggatgat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1508
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1508
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Met Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1509
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1509
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggaacat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1510
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1510
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Asn Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1511
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1511
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggcagat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1512
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1512
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Gln Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1513
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1513
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggcggat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1514
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1514
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Arg Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1515
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1515
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggagtat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1516
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1516
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1517
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1517
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggaccat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1518
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1518
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Thr Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1519
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1519
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcgggtgat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1520
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1520
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Val Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1521
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1521
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtggat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1522
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1522
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Trp Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1523
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1523
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtatat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1524
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1524
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Tyr Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1525
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1525
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1526
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1526
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1527
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1527
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaatttg tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1528
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1528
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Cys Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1529
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1529
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattga tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1530
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1530
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Asp Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1531
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1531
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattga ggtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1532
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1532
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Glu Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1533
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1533
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaatttt tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1534
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1534
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Phe Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1535
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1535
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattgg ggtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1536
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1536
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Gly Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1537
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1537
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattca cgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1538
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1538
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
His Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1539
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1539
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattat tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1540
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1540
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ile Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1541
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1541
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattaa ggtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1542
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1542
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Lys Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1543
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1543
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaatttt ggtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1544
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1544
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Leu Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1545
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1545
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattat ggtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1546
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1546
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Met Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1547
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1547
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattaa tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1548
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1548
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Asn Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1549
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1549
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattca ggtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1550
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1550
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Gln Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1551
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1551
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattag ggtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1552
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1552
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Arg Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1553
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1553
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattag tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1554
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1554
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ser Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1555
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1555
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattac ggtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1556
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1556
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Thr Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1557
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1557
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattgt ggtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1558
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1558
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Val Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1559
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1559
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaatttg ggtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1560
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1560
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Trp Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1561
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1561
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattta tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1562
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1562
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Tyr Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1563
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1563
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtgc gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1564
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1564
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Ala Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1565
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1565
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttg caatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1566
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1566
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Cys Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1567
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1567
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtga taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1568
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1568
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Asp Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1569
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1569
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtga gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1570
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1570
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Glu Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1571
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1571
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1572
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1572
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1573
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1573
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtgg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1574
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1574
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Gly Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1575
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1575
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtca taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1576
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1576
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser His Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1577
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1577
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtat taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1578
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1578
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Ile Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1579
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1579
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtaa gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1580
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1580
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Lys Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1581
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1581
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtct gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1582
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1582
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Leu Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1583
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1583
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtat gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1584
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1584
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Met Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1585
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1585
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtaa taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1586
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1586
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Asn Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1587
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1587
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcc taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1588
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1588
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Pro Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1589
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1589
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtca gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1590
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1590
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Gln Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1591
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1591
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtag taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1592
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1592
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Ser Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1593
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1593
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtac taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1594
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1594
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Thr Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1595
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1595
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtgt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1596
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1596
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Val Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1597
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1597
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1598
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1598
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Trp Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1599
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1599
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctaga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtta caatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1600
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1600
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Tyr Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1601
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1601
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaag ctatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1602
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1602
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Ala Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1603
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1603
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaat gtatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1604
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1604
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Cys Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1605
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1605
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaag atatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1606
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1606
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Asp Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1607
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1607
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaag agatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1608
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1608
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Glu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1609
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1609
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaat ttatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1610
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1610
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Phe Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1611
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1611
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaag gtatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1612
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1612
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Gly Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1613
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1613
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac atatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1614
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1614
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu His Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1615
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1615
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaaa ttatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1616
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1616
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Ile Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1617
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1617
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaaa agatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1618
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1618
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Lys Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1619
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1619
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac tgatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1620
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1620
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1621
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1621
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaaa atatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1622
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1622
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Asn Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1623
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1623
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac cgatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1624
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1624
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Pro Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1625
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1625
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac agatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1626
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1626
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Gln Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1627
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1627
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaaa gtatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1628
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1628
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Ser Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1629
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1629
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaaa ccatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1630
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1630
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Thr Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1631
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1631
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaag ttatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1632
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1632
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Val Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1633
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1633
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaat ggatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1634
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1634
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Trp Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1635
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1635
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaat atatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1636
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1636
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Tyr Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1637
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1637
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg caggcgtcac tgaaggacct cctg       1194

 
<210> 1638
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1638
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Ala Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1639
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1639
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagtgttcac tgaaggacct cctg       1194

 
<210> 1640
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1640
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Cys Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1641
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1641
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg caggactcac tgaaggacct cctg       1194

 
<210> 1642
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1642
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Asp Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1643
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1643
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg caggagtcac tgaaggacct cctg       1194

 
<210> 1644
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1644
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Glu Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1645
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1645
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagttttcac tgaaggacct cctg       1194

 
<210> 1646
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1646
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Phe Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1647
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1647
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagggttcac tgaaggacct cctg       1194

 
<210> 1648
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1648
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Gly Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1649
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1649
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagcattcac tgaaggacct cctg       1194

 
<210> 1650
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1650
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln His Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1651
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1651
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatttcac tgaaggacct cctg       1194

 
<210> 1652
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1652
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Ile Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1653
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1653
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagaagtcac tgaaggacct cctg       1194

 
<210> 1654
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1654
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Lys Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1655
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1655
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagctgtcac tgaaggacct cctg       1194

 
<210> 1656
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1656
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Leu Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1657
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1657
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 1658
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1658
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1659
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1659
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagaattcac tgaaggacct cctg       1194

 
<210> 1660
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1660
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Asn Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1661
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1661
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagccttcac tgaaggacct cctg       1194

 
<210> 1662
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1662
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Pro Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1663
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1663
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagcagtcac tgaaggacct cctg       1194

 
<210> 1664
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1664
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Gln Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1665
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1665
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagcgttcac tgaaggacct cctg       1194

 
<210> 1666
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1666
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Arg Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1667
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1667
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagtcttcac tgaaggacct cctg       1194

 
<210> 1668
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1668
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Ser Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1669
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1669
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg caggtgtcac tgaaggacct cctg       1194

 
<210> 1670
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1670
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Val Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1671
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1671
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagtggtcac tgaaggacct cctg       1194

 
<210> 1672
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1672
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Trp Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1673
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1673
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagtattcac tgaaggacct cctg       1194

 
<210> 1674
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1674
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Tyr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1675
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1675
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtggc tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1676
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1676
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ala Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1677
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1677
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgtg tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1678
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1678
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Cys Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1679
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1679
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgga tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1680
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1680
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Asp Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1681
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1681
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgga ggagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1682
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1682
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Glu Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1683
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1683
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgtt tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1684
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1684
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Phe Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1685
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1685
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtggg ggagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1686
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1686
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Gly Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1687
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1687
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgca cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1688
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1688
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val His Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1689
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1689
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgat tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1690
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1690
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ile Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1691
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1691
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgaa ggagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1692
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1692
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Lys Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1693
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1693
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgtt ggagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1694
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1694
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Leu Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1695
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1695
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgat ggagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1696
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1696
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Met Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1697
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1697
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgaa tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1698
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1698
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Asn Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1699
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1699
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgcc ggagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1700
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1700
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Pro Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1701
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1701
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgca ggagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1702
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1702
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Gln Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1703
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1703
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgag ggagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1704
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1704
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Arg Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1705
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1705
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgtc tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1706
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1706
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1707
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1707
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtggt ggagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1708
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1708
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Val Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1709
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1709
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgtg ggagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1710
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1710
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Trp Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1711
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1711
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgta tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1712
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1712
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Tyr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1713
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1713
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaaa gtgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1714
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1714
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Ser Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1715
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1715
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacaccgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agaaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1716
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1716
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Thr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Glu 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1717
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1717
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacacgg acgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1718
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1718
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Thr Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1719
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1719
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggcctat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcttc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacacgg aagtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1720
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1720
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Thr Glu Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1721
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1721
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaaa tggtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt acacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1722
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1722
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Met Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1723
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1723
ctggataatg gattggctag aacacctcca atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacaccgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcatc cataaagagc atcttggact ggaccagtcg taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1724
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1724
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Thr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1725
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1725
ctggataatg gattggctag aacacctcca atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccaggtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt acacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1726
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1726
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Gln Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1727
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1727
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac acctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gtatggtgcg cgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggcctat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1728
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1728
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Thr Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Arg Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1729
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1729
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgtggaa 120
atggccgaag tgatggtgcg cgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
agccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacctgg ccgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1730
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1730
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Val Glu Met Ala Glu Val Met Val Arg Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ser Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Leu Ala Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1731
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1731
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttctggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaaa gtgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1732
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1732
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Leu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Ser Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1733
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1733
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggatttc cataaagagc atcttggact ggaccagtcg taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag acgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1734
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1734
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ile Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1735
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1735
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgtggaa 120
atggccgaag tgatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggcctat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcttc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacacgg acgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1736
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1736
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Val Glu Met Ala Glu Val Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Thr Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1737
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1737
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacgtagtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cagcagcaaa ggccctgctc 840
caagacaaaa gtgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1738
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1738
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Val Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Ala Ala Lys Ala Leu Leu Gln Asp Lys Ser Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1739
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1739
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacaccgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagaccctg atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt acacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1740
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1740
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Thr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Pro Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1741
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1741
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacaccgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaaa tggtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1742
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1742
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Thr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Met Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1743
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1743
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agttcatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag acgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1744
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1744
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys Phe Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1745
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1745
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agactgggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1746
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1746
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Leu Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1747
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1747
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
agccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1748
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1748
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ser Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1749
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1749
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc aagatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agaaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1750
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1750
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Lys Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Glu 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1751
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1751
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttcccta aaggcataag gcagctggca aacgtagtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1752
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1752
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro Lys Gly Ile Arg Gln Leu Ala Asn Val Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1753
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1753
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacaccgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcatc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc aagatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1754
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1754
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Thr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Lys Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1755
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1755
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacgtagtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggaatc cataaagagc atcttggact ggaccagtcg taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaaa gtgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1756
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1756
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Val Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Glu Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Ser Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1757
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1757
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
agccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacctgg acgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1758
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1758
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ser Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Leu Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1759
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1759
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac acctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1760
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1760
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Thr Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1761
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1761
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttcccta aaggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaaa gtgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agactgggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg caggcgtcac tgaaggacct cctg       1194

 
<210> 1762
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1762
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro Lys Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Ser Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Leu Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Ala Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1763
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1763
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgtggaa 120
atggccgaag tgatggtgcg cgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1764
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1764
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Val Glu Met Ala Glu Val Met Val Arg Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1765
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1765
ctggataatg gattggctag aacacctcca atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttcccta aaggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agaaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg caggcgtcac tgaaggacct cctg       1194

 
<210> 1766
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1766
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro Lys Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Glu 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Ala Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1767
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1767
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggacatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1768
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1768
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly His Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1769
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1769
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac acctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggcctat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacctgg ccgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1770
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1770
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Thr Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Leu Ala Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1771
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1771
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc aagatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1772
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1772
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Lys Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1773
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1773
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agaaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagtggtcac tgaaggacct cctg       1194

 
<210> 1774
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1774
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Glu 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Trp Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1775
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1775
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaag tgatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggcctat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1776
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1776
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Val Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1777
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1777
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggcaatc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc agtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1778
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1778
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Gln Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1779
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1779
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttctggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1780
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1780
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Leu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1781
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1781
ctggataatg gattggctag aacacctcca atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttcccta aaggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggacatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1782
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1782
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro Lys Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly His Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1783
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1783
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacaccgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacgtcg atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagtggtcac tgaaggacct cctg       1194

 
<210> 1784
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1784
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Thr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Val Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Trp Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1785
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1785
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gtatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggcctat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacctgg acgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1786
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1786
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Leu Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1787
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1787
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcgggatat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacctgg ccgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1788
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1788
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Asp Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Leu Ala Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1789
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1789
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac acctgcatct ccgagaagct ctttgtggaa 120
atggccgaag tgatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcgggatat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agatatggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1790
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1790
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Thr Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Val Glu Met Ala Glu Val Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Asp Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Tyr Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1791
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1791
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc agtaaacaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1792
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1792
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Asn Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1793
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1793
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt gcacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1794
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1794
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Cys Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1795
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1795
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttctggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacgtagtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1796
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1796
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Leu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Val Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1797
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1797
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agttcatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag acgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agaaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1798
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1798
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys Phe Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Glu 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1799
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1799
ctggataatg gattggctag aacacctcca atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacgtagtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1800
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1800
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Val Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1801
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1801
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacgtagtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaaa gtgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1802
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1802
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Val Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Ser Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1803
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1803
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaagg aagtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1804
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1804
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Glu Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1805
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1805
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagtggtcac tgaaggacct cctg       1194

 
<210> 1806
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1806
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Trp Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1807
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1807
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacgtagtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggacatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1808
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1808
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Val Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly His Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1809
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1809
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagaccctg atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagtggtcac tgaaggacct cctg       1194

 
<210> 1810
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1810
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Pro Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Trp Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1811
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1811
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggacctc cataaagagc atcttggact ggaccagtcg taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt gcacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1812
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1812
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Thr Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Cys Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1813
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1813
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agaaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1814
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1814
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Glu 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1815
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1815
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgtggaa 120
atggccgaac gtatggtgcg cgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggcctat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
agccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1816
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1816
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Val Glu Met Ala Glu Arg Met Val Arg Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ser Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1817
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1817
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cagcagcaaa ggccctgctc 840
caagacaaag acgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1818
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1818
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Ala Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1819
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1819
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacgtagtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggtactc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cagcagcaaa ggccctgctc 840
caagacaaag acgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1820
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1820
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Val Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Tyr Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Ala Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1821
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1821
ctggataatg gattggctag aacacctggt atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1822
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1822
Leu Asp Asn Gly Leu Ala Arg Thr Pro Gly Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1823
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1823
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacaccgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggaatc cataaagagc atcttggact ggaccagtaa caatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1824
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1824
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Thr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Glu Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Asn Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1825
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1825
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg caggcgtcac tgaaggacct cctg       1194

 
<210> 1826
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1826
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Ala Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1827
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1827
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg caggactcac tgaaggacct cctg       1194

 
<210> 1828
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1828
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Asp Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1829
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1829
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgtggaa 120
atggccgaag tgatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggcctat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc agtaaacaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1830
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1830
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Val Glu Met Ala Glu Val Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Asn Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1831
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1831
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgtggaa 120
atggccgaag tgatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggcctat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1832
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1832
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Val Glu Met Ala Glu Val Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1833
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1833
ctggataatg gattggctag aacacctcca atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggtactc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1834
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1834
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Tyr Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1835
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1835
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacaccgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcatc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc aagatctcac cacaggcaag ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1836
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1836
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Thr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Lys Ile 
            260                 265                 270         
Ser Pro Gln Ala Arg Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1837
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1837
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacaccgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggaatc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaacgggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt acacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agaaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1838
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1838
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Thr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Glu Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Thr Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Glu 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1839
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1839
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggaatc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1840
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1840
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Glu Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1841
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1841
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttcccta aaggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1842
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1842
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro Lys Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1843
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1843
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcgggatat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1844
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1844
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Asp Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1845
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1845
ctggataatg gattggctag aacacctcca atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggaatc cataaagagc atcttggact ggaccagtaa caatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1846
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1846
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Glu Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Asn Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1847
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1847
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agttcatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtat taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1848
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1848
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys Phe Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Ile Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1849
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1849
ctggataatg gattggctag aacacctggt atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccatgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcatc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt acacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg caggactcac tgaaggacct cctg       1194

 
<210> 1850
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1850
Leu Asp Asn Gly Leu Ala Arg Thr Pro Gly Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Asp Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1851
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1851
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttctggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggaatc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1852
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1852
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Leu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Glu Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1853
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1853
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agatatggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1854
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1854
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Tyr Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1855
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1855
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacgtagtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agactgggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1856
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1856
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Val Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Leu Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1857
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1857
ctggataatg gattggctag aacacctcca atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacaccgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcatc cataaagagc atcttggact ggaccagtcg taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agactgggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1858
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1858
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Thr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Leu Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1859
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1859
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcgggatat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agatatggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1860
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1860
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Asp Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Tyr Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395             

 
<210> 1861
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1861
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaag tgatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggcctat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaagg aagtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agatatggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1862
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1862
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Val Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Glu Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Tyr Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395    


<210> 1863
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1863
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcgggatat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacacgg acgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 1864
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 1864
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Asp Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Thr Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395     