                         SEQUENCE LISTING

<110>  GEORGE WASHINGTON UNIVERSITY
        
 
<120>  COBRA1/NELF-B as a Booster for Efficacy of CD8+ T Cell-Based 
       Therapy

<130>  3973.015PC01

<150>  US 62/982,514
<151>  2020-02-27

<160>  10    
<170>  PatentIn version 3.5

<210>  1
<211>  1887
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  COBRA1 nucleic acid sequence

<400>  1
ctggccgagc tggagggcgc cggggagcgg ggctcgggcg gtccccgagg cccggcggag       60

cgggcttctg gggtgtctgc ggcggcgccg ggggaacggg ctggggatgg ggcgcctagc      120

cgggcggtgg ccggggcctc ggccatgttc gcggggctgc aggacctggg cgtggccaac      180

ggcgaggacc tgaaggagac cctgaccaac tgcacggagc cgctcaaggc catcgagcag      240

ttccagacag agaatggtgt gctgctgcca tctcttcagt cagccctccc cttcttggac      300

ctgcacggga cgccgcggct ggagttccac cagtcggtat tcgatgagct gcgggacaag      360

ctgctggagc gagtgtcagc catcgcttcg gaggggaagg ctgaggaaag gtacaagaag      420

ctggaagacc ttctggagaa gagcttttct ctggtgaaga tgccgtccct gcagcccgtg      480

gtgatgtgcg tcatgaagca cctgcccaag gttccggaga aaaaactgaa gctggttatg      540

gctgacaagg agctgtatcg agcctgcgcc gtggaggtga agcggcagat ctggcaagac      600

aaccaggccc tcttcgggga cgaggtttcc ccactcctga agcagtacat cctggagaag      660

gagagcgctc tcttcagtac agagctctct gtcctgcaca actttttcag tccttccccc      720

aagaccaggc gccagggcga ggtggtgcag cggctgacgc ggatggtggg gaagaacgtg      780

aagctgtacg acatggtgct gcagtttctg cgcacgctct tcctgcgcac gcggaatgtg      840

cactactgca cgctgcgggc tgagctgctc atgtccctgc acgacctgga cgtgggtgaa      900

atctgcaccg tggacccgtg ccacaagttc acctggtgcc tggacgcctg catccgagag      960

cggttcgtgg acagcaagag ggcgcgggag ctgcaggggt ttctcgatgg cgtcaagaag     1020

ggccaggagc aggtgctggg ggacctgtcc atgatcctgt gtgacccctt cgccatcaac     1080

acgctggcac tgagcacagt caggcacctg caggagctgg tcggccagga gacactgccc     1140

agggacagcc ccgacctcct gctgctgctc cggctgctgg cgctgggcca gggagcctgg     1200

gacatgatcg acagccaggt cttcaaggag cccaagatgg aggtagagct catcaccagg     1260

ttcctcccga tgctcatgtc cttcctggtg gatgactaca ctttcaatgt ggatcagaaa     1320

cttccggctg aggagaaagc cccagtctca tatccaaaca cacttcccga aagcttcact     1380

aagtttctgc aggagcagcg catggcctgc gaggtggggc tgtactacgt cctgcacatc     1440

accaagcaga ggaacaagaa cgcgctcctc cgcctgctgc ccgggctggt ggagaccttt     1500

ggcgacttgg cctttggcga catcttcctc cacctgctca cgggcaacct tgcgctgctg     1560

gccgacgaat ttgcccttga ggacttctgc agcagcctct tcgatggctt cttcctcacc     1620

gcctctccaa ggaaggagaa cgtgcaccgg cacgcgctgc ggctcctcat tcacctgcac     1680

cccagggtgg ccccgtctaa gctggaggcg ttgcagaagg ccctggagcc tacaggccag     1740

agcggagagg cagtgaagga gctttactcc cagctcggcg agaagctgga acagctggat     1800

caccggaagc ccagcccggc acaggctgcg gagacgccgg ccctggagct gcccctcccc     1860

agcgtgcccg cccctgcccc gctctga                                         1887


<210>  2
<211>  628
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  COBRA1 amino acid sequence

<400>  2

Met Ala Glu Leu Glu Gly Ala Gly Glu Arg Gly Ser Gly Gly Pro Arg 
1               5                   10                  15      


Gly Pro Ala Glu Arg Ala Ser Gly Val Ser Ala Ala Ala Pro Gly Glu 
            20                  25                  30          


Arg Ala Gly Asp Gly Ala Pro Ser Arg Ala Val Ala Gly Ala Ser Ala 
        35                  40                  45              


Met Phe Ala Gly Leu Gln Asp Leu Gly Val Ala Asn Gly Glu Asp Leu 
    50                  55                  60                  


Lys Glu Thr Leu Thr Asn Cys Thr Glu Pro Leu Lys Ala Ile Glu Gln 
65                  70                  75                  80  


Phe Gln Thr Glu Asn Gly Val Leu Leu Pro Ser Leu Gln Ser Ala Leu 
                85                  90                  95      


Pro Phe Leu Asp Leu His Gly Thr Pro Arg Leu Glu Phe His Gln Ser 
            100                 105                 110         


Val Phe Asp Glu Leu Arg Asp Lys Leu Leu Glu Arg Val Ser Ala Ile 
        115                 120                 125             


Ala Ser Glu Gly Lys Ala Glu Glu Arg Tyr Lys Lys Leu Glu Asp Leu 
    130                 135                 140                 


Leu Glu Lys Ser Phe Ser Leu Val Lys Met Pro Ser Leu Gln Pro Val 
145                 150                 155                 160 


Val Met Cys Val Met Lys His Leu Pro Lys Val Pro Glu Lys Lys Leu 
                165                 170                 175     


Lys Leu Val Met Ala Asp Lys Glu Leu Tyr Arg Ala Cys Ala Val Glu 
            180                 185                 190         


Val Lys Arg Gln Ile Trp Gln Asp Asn Gln Ala Leu Phe Gly Asp Glu 
        195                 200                 205             


Val Ser Pro Leu Leu Lys Gln Tyr Ile Leu Glu Lys Glu Ser Ala Leu 
    210                 215                 220                 


Phe Ser Thr Glu Leu Ser Val Leu His Asn Phe Phe Ser Pro Ser Pro 
225                 230                 235                 240 


Lys Thr Arg Arg Gln Gly Glu Val Val Gln Arg Leu Thr Arg Met Val 
                245                 250                 255     


Gly Lys Asn Val Lys Leu Tyr Asp Met Val Leu Gln Phe Leu Arg Thr 
            260                 265                 270         


Leu Phe Leu Arg Thr Arg Asn Val His Tyr Cys Thr Leu Arg Ala Glu 
        275                 280                 285             


Leu Leu Met Ser Leu His Asp Leu Asp Val Gly Glu Ile Cys Thr Val 
    290                 295                 300                 


Asp Pro Cys His Lys Phe Thr Trp Cys Leu Asp Ala Cys Ile Arg Glu 
305                 310                 315                 320 


Arg Phe Val Asp Ser Lys Arg Ala Arg Glu Leu Gln Gly Phe Leu Asp 
                325                 330                 335     


Gly Val Lys Lys Gly Gln Glu Gln Val Leu Gly Asp Leu Ser Met Ile 
            340                 345                 350         


Leu Cys Asp Pro Phe Ala Ile Asn Thr Leu Ala Leu Ser Thr Val Arg 
        355                 360                 365             


His Leu Gln Glu Leu Val Gly Gln Glu Thr Leu Pro Arg Asp Ser Pro 
    370                 375                 380                 


Asp Leu Leu Leu Leu Leu Arg Leu Leu Ala Leu Gly Gln Gly Ala Trp 
385                 390                 395                 400 


Asp Met Ile Asp Ser Gln Val Phe Lys Glu Pro Lys Met Glu Val Glu 
                405                 410                 415     


Leu Ile Thr Arg Phe Leu Pro Met Leu Met Ser Phe Leu Val Asp Asp 
            420                 425                 430         


Tyr Thr Phe Asn Val Asp Gln Lys Leu Pro Ala Glu Glu Lys Ala Pro 
        435                 440                 445             


Val Ser Tyr Pro Asn Thr Leu Pro Glu Ser Phe Thr Lys Phe Leu Gln 
    450                 455                 460                 


Glu Gln Arg Met Ala Cys Glu Val Gly Leu Tyr Tyr Val Leu His Ile 
465                 470                 475                 480 


Thr Lys Gln Arg Asn Lys Asn Ala Leu Leu Arg Leu Leu Pro Gly Leu 
                485                 490                 495     


Val Glu Thr Phe Gly Asp Leu Ala Phe Gly Asp Ile Phe Leu His Leu 
            500                 505                 510         


Leu Thr Gly Asn Leu Ala Leu Leu Ala Asp Glu Phe Ala Leu Glu Asp 
        515                 520                 525             


Phe Cys Ser Ser Leu Phe Asp Gly Phe Phe Leu Thr Ala Ser Pro Arg 
    530                 535                 540                 


Lys Glu Asn Val His Arg His Ala Leu Arg Leu Leu Ile His Leu His 
545                 550                 555                 560 


Pro Arg Val Ala Pro Ser Lys Leu Glu Ala Leu Gln Lys Ala Leu Glu 
                565                 570                 575     


Pro Thr Gly Gln Ser Gly Glu Ala Val Lys Glu Leu Tyr Ser Gln Leu 
            580                 585                 590         


Gly Glu Lys Leu Glu Gln Leu Asp His Arg Lys Pro Ser Pro Ala Gln 
        595                 600                 605             


Ala Ala Glu Thr Pro Ala Leu Glu Leu Pro Leu Pro Ser Val Pro Ala 
    610                 615                 620                 


Pro Ala Pro Leu 
625             


<210>  3
<211>  1587
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (NELFA nucleic acid sequence)

<400>  3
atggcgtcca tgcgggagag cgacacgggc ctgtggctgc acaacaagct gggggccacg       60

gacgagctgt gggcgccgcc cagcatcgcg tccctgctca cggccgcggt catcgacaac      120

atccgtctct gcttccatgg cctctcgtcg gcagtgaagc tcaagttgct actcgggacg      180

ctgcacctcc cgcgccgcac ggtggacgag atgaagggcg ccctaatgga gatcatccag      240

ctcgccagcc tcgactcgga cccctgggtg ctcatggtcg ccgacatctt gaagtccttt      300

ccggacacag gctcgcttaa cctggagctg gaggagcaga atcccaacgt tcaggatatt      360

ttgggagaac ttagagaaaa ggtgggtgag tgtgaagcgt ctgccatgct gccactggag      420

tgccagtact tgaacaaaaa cgccctgacg accctcgcgg gacccctcac tcccccggtg      480

aagcattttc agttaaagcg gaaacccaag agcgccacgc tgcgggcgga gctgctgcag      540

aagtccacgg agaccgccca gcagttgaag cggagcgccg gggtgccctt ccacgccaag      600

ggccgggggc tgctgcggaa gatggacacc accaccccac tcaaaggcat cccgaagcag      660

gcgcccttca gaagccccac ggcgcccagc gtcttcagcc ccacagggaa ccggaccccc      720

atcccgcctt ccaggacgct gctgcggaag gaacgaggtg tgaagctgct ggacatctct      780

gagctggata tggttggcgc tggccgagag gcgaagcgga gaaggaagac tctcgatgcg      840

gaggtggtgg agaagccggc caaggaggaa acggtggtgg agaacgccac cccggactac      900

gcagccggcc tggtgtccac gcagaaactt gggtccctga acaatgagcc tgcgctgccc      960

tccacgagct accttccctc cacgcccagc gtggttcccg cctcctccta catccccagc     1020

tccgagacgc ccccagcccc atcttcccgg gaagccagcc gcccaccaga ggagcccagc     1080

gccccgagcc ccacgttgcc agcgcagttc aagcagcggg cgcccatgta caacagcggc     1140

ctgagccctg ccacacccac gcctgcggcg cccacctcgc ctctgacacc caccacacct     1200

ccggctgtcg cccctaccac tcagacaccc ccggttgcca tggtggcccc gcagacccag     1260

gcccctgctc agcagcagcc taagaagaac ctgtccctca cgagagagca gatgttcgct     1320

gcccaggaga tgttcaagac ggccaacaaa gtcacgcggc ccgagaaggc cctcatcctg     1380

ggcttcatgg ccggctcccg agagaacccg tgccaggagc agggggacgt gatccagatc     1440

aagctgagcg agcacacgga ggacctgccc aaggcggacg gccagggtag cacaaccatg     1500

ctggtggaca cagtgtttga gatgaactat gccacgggcc agtggacgcg cttcaagaag     1560

tacaagccca tgaccaatgt gtcctag                                         1587


<210>  4
<211>  528
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NELFA amino acid sequence

<400>  4

Met Ala Ser Met Arg Glu Ser Asp Thr Gly Leu Trp Leu His Asn Lys 
1               5                   10                  15      


Leu Gly Ala Thr Asp Glu Leu Trp Ala Pro Pro Ser Ile Ala Ser Leu 
            20                  25                  30          


Leu Thr Ala Ala Val Ile Asp Asn Ile Arg Leu Cys Phe His Gly Leu 
        35                  40                  45              


Ser Ser Ala Val Lys Leu Lys Leu Leu Leu Gly Thr Leu His Leu Pro 
    50                  55                  60                  


Arg Arg Thr Val Asp Glu Met Lys Gly Ala Leu Met Glu Ile Ile Gln 
65                  70                  75                  80  


Leu Ala Ser Leu Asp Ser Asp Pro Trp Val Leu Met Val Ala Asp Ile 
                85                  90                  95      


Leu Lys Ser Phe Pro Asp Thr Gly Ser Leu Asn Leu Glu Leu Glu Glu 
            100                 105                 110         


Gln Asn Pro Asn Val Gln Asp Ile Leu Gly Glu Leu Arg Glu Lys Val 
        115                 120                 125             


Gly Glu Cys Glu Ala Ser Ala Met Leu Pro Leu Glu Cys Gln Tyr Leu 
    130                 135                 140                 


Asn Lys Asn Ala Leu Thr Thr Leu Ala Gly Pro Leu Thr Pro Pro Val 
145                 150                 155                 160 


Lys His Phe Gln Leu Lys Arg Lys Pro Lys Ser Ala Thr Leu Arg Ala 
                165                 170                 175     


Glu Leu Leu Gln Lys Ser Thr Glu Thr Ala Gln Gln Leu Lys Arg Ser 
            180                 185                 190         


Ala Gly Val Pro Phe His Ala Lys Gly Arg Gly Leu Leu Arg Lys Met 
        195                 200                 205             


Asp Thr Thr Thr Pro Leu Lys Gly Ile Pro Lys Gln Ala Pro Phe Arg 
    210                 215                 220                 


Ser Pro Thr Ala Pro Ser Val Phe Ser Pro Thr Gly Asn Arg Thr Pro 
225                 230                 235                 240 


Ile Pro Pro Ser Arg Thr Leu Leu Arg Lys Glu Arg Gly Val Lys Leu 
                245                 250                 255     


Leu Asp Ile Ser Glu Leu Asp Met Val Gly Ala Gly Arg Glu Ala Lys 
            260                 265                 270         


Arg Arg Arg Lys Thr Leu Asp Ala Glu Val Val Glu Lys Pro Ala Lys 
        275                 280                 285             


Glu Glu Thr Val Val Glu Asn Ala Thr Pro Asp Tyr Ala Ala Gly Leu 
    290                 295                 300                 


Val Ser Thr Gln Lys Leu Gly Ser Leu Asn Asn Glu Pro Ala Leu Pro 
305                 310                 315                 320 


Ser Thr Ser Tyr Leu Pro Ser Thr Pro Ser Val Val Pro Ala Ser Ser 
                325                 330                 335     


Tyr Ile Pro Ser Ser Glu Thr Pro Pro Ala Pro Ser Ser Arg Glu Ala 
            340                 345                 350         


Ser Arg Pro Pro Glu Glu Pro Ser Ala Pro Ser Pro Thr Leu Pro Ala 
        355                 360                 365             


Gln Phe Lys Gln Arg Ala Pro Met Tyr Asn Ser Gly Leu Ser Pro Ala 
    370                 375                 380                 


Thr Pro Thr Pro Ala Ala Pro Thr Ser Pro Leu Thr Pro Thr Thr Pro 
385                 390                 395                 400 


Pro Ala Val Ala Pro Thr Thr Gln Thr Pro Pro Val Ala Met Val Ala 
                405                 410                 415     


Pro Gln Thr Gln Ala Pro Ala Gln Gln Gln Pro Lys Lys Asn Leu Ser 
            420                 425                 430         


Leu Thr Arg Glu Gln Met Phe Ala Ala Gln Glu Met Phe Lys Thr Ala 
        435                 440                 445             


Asn Lys Val Thr Arg Pro Glu Lys Ala Leu Ile Leu Gly Phe Met Ala 
    450                 455                 460                 


Gly Ser Arg Glu Asn Pro Cys Gln Glu Gln Gly Asp Val Ile Gln Ile 
465                 470                 475                 480 


Lys Leu Ser Glu His Thr Glu Asp Leu Pro Lys Ala Asp Gly Gln Gly 
                485                 490                 495     


Ser Thr Thr Met Leu Val Asp Thr Val Phe Glu Met Asn Tyr Ala Thr 
            500                 505                 510         


Gly Gln Trp Thr Arg Phe Lys Lys Tyr Lys Pro Met Thr Asn Val Ser 
        515                 520                 525             


<210>  5
<211>  1773
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (NELFC nucleic acid sequence)

<400>  5
atggcggggg ccgtgccggg cgccatcatg gacgaggact actacgggag cgcggccgag       60

tggggcgacg aggctgacgg cggccagcag gaggatgatt ctggagaagg agaggatgat      120

gcggaggttc agcaagaatg cctgcataaa ttttccaccc gggattatat catggaaccc      180

tccatcttca acactctgaa gaggtatttt caggcaggag ggtctccaga gaatgttatc      240

cagctcttat ctgaaaacta caccgctgtg gcccagactg tgaacctgct ggccgagtgg      300

ctcattcaga caggtgttga gccagtgcag gttcaggaaa ctgtggaaaa tcacttgaag      360

agtttgctga tcaaacattt tgacccccgc aaagcagatt ctatttttac tgaagaagga      420

gagaccccag cgtggctgga acagatgatt gcacatacca cgtggcggga ccttttttat      480

aaactggctg aagcccatcc agactgtttg atgctgaact tcaccgttaa gcttatttct      540

gacgcagggt accaggggga gatcaccagt gtgtccacag catgccagca gctagaagtg      600

ttctcgagag tgctccggac ctctctagct acaattttag atggaggaga agaaaacctt      660

gaaaaaaatc tccctgagtt tgccaagatg gtgtgccacg gggagcacac gtacctgttt      720

gcccaggcca tgatgtccgt gctggcccag gaggagcagg ggggctccgc tgtgcgcagg      780

atcgcccagg aagtgcagcg ctttgcccag gagaaaggtc atgacgccag tcagatcaca      840

ctagccttgg gcacagctgc ctcctacccc agggcctgcc aggctctcgg ggccatgctg      900

tccaaaggag ccctgaaccc tgctgacatc accgtcctgt tcaagatgtt cacaagcatg      960

gaccctcctc cggttgaact tatccgcgtt ccagccttcc tggacctgtt catgcagtca     1020

ctctttaaac caggggctcg gatcaaccag gaccacaagc acaaatacat ccacatcttg     1080

gcgtacgcag caagcgtggt tgagacctgg aagaagaaca agcgagtgag catcaataaa     1140

gatgagctga agtcaacgtc aaaagctgtc gaaaccgttc acaatttgtg ttgcaacgag     1200

aacaaagggg cctctgaact agtggcagaa ttgagcacac tttatcagtg tattaggttt     1260

ccagtggtag caatgggtgt gctgaagtgg gtggattgga ctgtatcaga accaaggtac     1320

tttcagctgc agactgacca tacccctgtc cacctggcgt tgctggatga gatcagcacc     1380

tgccaccagc tcctgcaccc ccaggtcctg cagctgcttg ttaagctttt tgagactgag     1440

cactcccagc tggacgtgat ggagcagctt gagttgaaga agacactgct ggacaggatg     1500

gttcacctgc tgagtcgagg ttatgtactt cctgttgtca gttacatccg aaagtgtctg     1560

gagaagctgg acactgacat ttcactcatt cgctattttg tcactgaggt gctggacgtc     1620

attgctcctc cttatacctc tgacttcgtg caacttttcc tccccatcct ggagaatgac     1680

agcatcgcag gtaccatcaa aacggaaggc gagcatgacc ctgtgacgga gtttatagct     1740

cactgcaaat ctaacttcat catggtgaac taa                                  1773


<210>  6
<211>  590
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NELFC amino acid sequence

<400>  6

Met Ala Gly Ala Val Pro Gly Ala Ile Met Asp Glu Asp Tyr Tyr Gly 
1               5                   10                  15      


Ser Ala Ala Glu Trp Gly Asp Glu Ala Asp Gly Gly Gln Gln Glu Asp 
            20                  25                  30          


Asp Ser Gly Glu Gly Glu Asp Asp Ala Glu Val Gln Gln Glu Cys Leu 
        35                  40                  45              


His Lys Phe Ser Thr Arg Asp Tyr Ile Met Glu Pro Ser Ile Phe Asn 
    50                  55                  60                  


Thr Leu Lys Arg Tyr Phe Gln Ala Gly Gly Ser Pro Glu Asn Val Ile 
65                  70                  75                  80  


Gln Leu Leu Ser Glu Asn Tyr Thr Ala Val Ala Gln Thr Val Asn Leu 
                85                  90                  95      


Leu Ala Glu Trp Leu Ile Gln Thr Gly Val Glu Pro Val Gln Val Gln 
            100                 105                 110         


Glu Thr Val Glu Asn His Leu Lys Ser Leu Leu Ile Lys His Phe Asp 
        115                 120                 125             


Pro Arg Lys Ala Asp Ser Ile Phe Thr Glu Glu Gly Glu Thr Pro Ala 
    130                 135                 140                 


Trp Leu Glu Gln Met Ile Ala His Thr Thr Trp Arg Asp Leu Phe Tyr 
145                 150                 155                 160 


Lys Leu Ala Glu Ala His Pro Asp Cys Leu Met Leu Asn Phe Thr Val 
                165                 170                 175     


Lys Leu Ile Ser Asp Ala Gly Tyr Gln Gly Glu Ile Thr Ser Val Ser 
            180                 185                 190         


Thr Ala Cys Gln Gln Leu Glu Val Phe Ser Arg Val Leu Arg Thr Ser 
        195                 200                 205             


Leu Ala Thr Ile Leu Asp Gly Gly Glu Glu Asn Leu Glu Lys Asn Leu 
    210                 215                 220                 


Pro Glu Phe Ala Lys Met Val Cys His Gly Glu His Thr Tyr Leu Phe 
225                 230                 235                 240 


Ala Gln Ala Met Met Ser Val Leu Ala Gln Glu Glu Gln Gly Gly Ser 
                245                 250                 255     


Ala Val Arg Arg Ile Ala Gln Glu Val Gln Arg Phe Ala Gln Glu Lys 
            260                 265                 270         


Gly His Asp Ala Ser Gln Ile Thr Leu Ala Leu Gly Thr Ala Ala Ser 
        275                 280                 285             


Tyr Pro Arg Ala Cys Gln Ala Leu Gly Ala Met Leu Ser Lys Gly Ala 
    290                 295                 300                 


Leu Asn Pro Ala Asp Ile Thr Val Leu Phe Lys Met Phe Thr Ser Met 
305                 310                 315                 320 


Asp Pro Pro Pro Val Glu Leu Ile Arg Val Pro Ala Phe Leu Asp Leu 
                325                 330                 335     


Phe Met Gln Ser Leu Phe Lys Pro Gly Ala Arg Ile Asn Gln Asp His 
            340                 345                 350         


Lys His Lys Tyr Ile His Ile Leu Ala Tyr Ala Ala Ser Val Val Glu 
        355                 360                 365             


Thr Trp Lys Lys Asn Lys Arg Val Ser Ile Asn Lys Asp Glu Leu Lys 
    370                 375                 380                 


Ser Thr Ser Lys Ala Val Glu Thr Val His Asn Leu Cys Cys Asn Glu 
385                 390                 395                 400 


Asn Lys Gly Ala Ser Glu Leu Val Ala Glu Leu Ser Thr Leu Tyr Gln 
                405                 410                 415     


Cys Ile Arg Phe Pro Val Val Ala Met Gly Val Leu Lys Trp Val Asp 
            420                 425                 430         


Trp Thr Val Ser Glu Pro Arg Tyr Phe Gln Leu Gln Thr Asp His Thr 
        435                 440                 445             


Pro Val His Leu Ala Leu Leu Asp Glu Ile Ser Thr Cys His Gln Leu 
    450                 455                 460                 


Leu His Pro Gln Val Leu Gln Leu Leu Val Lys Leu Phe Glu Thr Glu 
465                 470                 475                 480 


His Ser Gln Leu Asp Val Met Glu Gln Leu Glu Leu Lys Lys Thr Leu 
                485                 490                 495     


Leu Asp Arg Met Val His Leu Leu Ser Arg Gly Tyr Val Leu Pro Val 
            500                 505                 510         


Val Ser Tyr Ile Arg Lys Cys Leu Glu Lys Leu Asp Thr Asp Ile Ser 
        515                 520                 525             


Leu Ile Arg Tyr Phe Val Thr Glu Val Leu Asp Val Ile Ala Pro Pro 
    530                 535                 540                 


Tyr Thr Ser Asp Phe Val Gln Leu Phe Leu Pro Ile Leu Glu Asn Asp 
545                 550                 555                 560 


Ser Ile Ala Gly Thr Ile Lys Thr Glu Gly Glu His Asp Pro Val Thr 
                565                 570                 575     


Glu Phe Ile Ala His Cys Lys Ser Asn Phe Ile Met Val Asn 
            580                 585                 590 


<210>  7
<211>  1746
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NELFD nucleic acid sequence

<400>  7
atggacgagg actactacgg gagcgcggcc gagtggggcg acgaggctga cggcggccag       60

caggaggatg attctggaga aggagaggat gatgcggagg ttcagcaaga atgcctgcat      120

aaattttcca cccgggatta tatcatggaa ccctccatct tcaacactct gaagaggtat      180

tttcaggcag gagggtctcc agagaatgtt atccagctct tatctgaaaa ctacaccgct      240

gtggcccaga ctgtgaacct gctggccgag tggctcattc agacaggtgt tgagccagtg      300

caggttcagg aaactgtgga aaatcacttg aagagtttgc tgatcaaaca ttttgacccc      360

cgcaaagcag attctatttt tactgaagaa ggagagaccc cagcgtggct ggaacagatg      420

attgcacata ccacgtggcg ggaccttttt tataaactgg ctgaagccca tccagactgt      480

ttgatgctga acttcaccgt taagcttatt tctgacgcag ggtaccaggg ggagatcacc      540

agtgtgtcca cagcatgcca gcagctagaa gtgttctcga gagtgctccg gacctctcta      600

gctacaattt tagatggagg agaagaaaac cttgaaaaaa atctccctga gtttgccaag      660

atggtgtgcc acggggagca cacgtacctg tttgcccagg ccatgatgtc cgtgctggcc      720

caggaggagc aggggggctc cgctgtgcgc aggatcgccc aggaagtgca gcgctttgcc      780

caggagaaag gtcatgacgc cagtcagatc acactagcct tgggcacagc tgcctcctac      840

cccagggcct gccaggctct cggggccatg ctgtccaaag gagccctgaa ccctgctgac      900

atcaccgtcc tgttcaagat gttcacaagc atggaccctc ctccggttga acttatccgc      960

gttccagcct tcctggacct gttcatgcag tcactcttta aaccaggggc tcggatcaac     1020

caggaccaca agcacaaata catccacatc ttggcgtacg cagcaagcgt ggttgagacc     1080

tggaagaaga acaagcgagt gagcatcaat aaagatgagc tgaagtcaac gtcaaaagct     1140

gtcgaaaccg ttcacaattt gtgttgcaac gagaacaaag gggcctctga actagtggca     1200

gaattgagca cactttatca gtgtattagg tttccagtgg tagcaatggg tgtgctgaag     1260

tgggtggatt ggactgtatc agaaccaagg tactttcagc tgcagactga ccatacccct     1320

gtccacctgg cgttgctgga tgagatcagc acctgccacc agctcctgca cccccaggtc     1380

ctgcagctgc ttgttaagct ttttgagact gagcactccc agctggacgt gatggagcag     1440

cttgagttga agaagacact gctggacagg atggttcacc tgctgagtcg aggttatgta     1500

cttcctgttg tcagttacat ccgaaagtgt ctggagaagc tggacactga catttcactc     1560

attcgctatt ttgtcactga ggtgctggac gtcattgctc ctccttatac ctctgacttc     1620

gtgcaacttt tcctccccat cctggagaat gacagcatcg caggtaccat caaaacggaa     1680

ggcgagcatg accctgtgac ggagtttata gctcactgca aatctaactt catcatggtg     1740

aactaa                                                                1746


<210>  8
<211>  581
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NELFD amino acid sequence

<400>  8

Met Asp Glu Asp Tyr Tyr Gly Ser Ala Ala Glu Trp Gly Asp Glu Ala 
1               5                   10                  15      


Asp Gly Gly Gln Gln Glu Asp Asp Ser Gly Glu Gly Glu Asp Asp Ala 
            20                  25                  30          


Glu Val Gln Gln Glu Cys Leu His Lys Phe Ser Thr Arg Asp Tyr Ile 
        35                  40                  45              


Met Glu Pro Ser Ile Phe Asn Thr Leu Lys Arg Tyr Phe Gln Ala Gly 
    50                  55                  60                  


Gly Ser Pro Glu Asn Val Ile Gln Leu Leu Ser Glu Asn Tyr Thr Ala 
65                  70                  75                  80  


Val Ala Gln Thr Val Asn Leu Leu Ala Glu Trp Leu Ile Gln Thr Gly 
                85                  90                  95      


Val Glu Pro Val Gln Val Gln Glu Thr Val Glu Asn His Leu Lys Ser 
            100                 105                 110         


Leu Leu Ile Lys His Phe Asp Pro Arg Lys Ala Asp Ser Ile Phe Thr 
        115                 120                 125             


Glu Glu Gly Glu Thr Pro Ala Trp Leu Glu Gln Met Ile Ala His Thr 
    130                 135                 140                 


Thr Trp Arg Asp Leu Phe Tyr Lys Leu Ala Glu Ala His Pro Asp Cys 
145                 150                 155                 160 


Leu Met Leu Asn Phe Thr Val Lys Leu Ile Ser Asp Ala Gly Tyr Gln 
                165                 170                 175     


Gly Glu Ile Thr Ser Val Ser Thr Ala Cys Gln Gln Leu Glu Val Phe 
            180                 185                 190         


Ser Arg Val Leu Arg Thr Ser Leu Ala Thr Ile Leu Asp Gly Gly Glu 
        195                 200                 205             


Glu Asn Leu Glu Lys Asn Leu Pro Glu Phe Ala Lys Met Val Cys His 
    210                 215                 220                 


Gly Glu His Thr Tyr Leu Phe Ala Gln Ala Met Met Ser Val Leu Ala 
225                 230                 235                 240 


Gln Glu Glu Gln Gly Gly Ser Ala Val Arg Arg Ile Ala Gln Glu Val 
                245                 250                 255     


Gln Arg Phe Ala Gln Glu Lys Gly His Asp Ala Ser Gln Ile Thr Leu 
            260                 265                 270         


Ala Leu Gly Thr Ala Ala Ser Tyr Pro Arg Ala Cys Gln Ala Leu Gly 
        275                 280                 285             


Ala Met Leu Ser Lys Gly Ala Leu Asn Pro Ala Asp Ile Thr Val Leu 
    290                 295                 300                 


Phe Lys Met Phe Thr Ser Met Asp Pro Pro Pro Val Glu Leu Ile Arg 
305                 310                 315                 320 


Val Pro Ala Phe Leu Asp Leu Phe Met Gln Ser Leu Phe Lys Pro Gly 
                325                 330                 335     


Ala Arg Ile Asn Gln Asp His Lys His Lys Tyr Ile His Ile Leu Ala 
            340                 345                 350         


Tyr Ala Ala Ser Val Val Glu Thr Trp Lys Lys Asn Lys Arg Val Ser 
        355                 360                 365             


Ile Asn Lys Asp Glu Leu Lys Ser Thr Ser Lys Ala Val Glu Thr Val 
    370                 375                 380                 


His Asn Leu Cys Cys Asn Glu Asn Lys Gly Ala Ser Glu Leu Val Ala 
385                 390                 395                 400 


Glu Leu Ser Thr Leu Tyr Gln Cys Ile Arg Phe Pro Val Val Ala Met 
                405                 410                 415     


Gly Val Leu Lys Trp Val Asp Trp Thr Val Ser Glu Pro Arg Tyr Phe 
            420                 425                 430         


Gln Leu Gln Thr Asp His Thr Pro Val His Leu Ala Leu Leu Asp Glu 
        435                 440                 445             


Ile Ser Thr Cys His Gln Leu Leu His Pro Gln Val Leu Gln Leu Leu 
    450                 455                 460                 


Val Lys Leu Phe Glu Thr Glu His Ser Gln Leu Asp Val Met Glu Gln 
465                 470                 475                 480 


Leu Glu Leu Lys Lys Thr Leu Leu Asp Arg Met Val His Leu Leu Ser 
                485                 490                 495     


Arg Gly Tyr Val Leu Pro Val Val Ser Tyr Ile Arg Lys Cys Leu Glu 
            500                 505                 510         


Lys Leu Asp Thr Asp Ile Ser Leu Ile Arg Tyr Phe Val Thr Glu Val 
        515                 520                 525             


Leu Asp Val Ile Ala Pro Pro Tyr Thr Ser Asp Phe Val Gln Leu Phe 
    530                 535                 540                 


Leu Pro Ile Leu Glu Asn Asp Ser Ile Ala Gly Thr Ile Lys Thr Glu 
545                 550                 555                 560 


Gly Glu His Asp Pro Val Thr Glu Phe Ile Ala His Cys Lys Ser Asn 
                565                 570                 575     


Phe Ile Met Val Asn 
            580     


<210>  9
<211>  1143
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NELFE nucleic acid sequence

<400>  9
atgttggtga taccccccgg actgagcgag gaagaggagg ctctgcagaa gaaattcaac       60

aagctcaaga aaaagaaaaa ggcattgctg gctctgaaga agcaaagtag cagcagcaca      120

accagccaag gtggtgtcaa acgctcacta tcagagcagc ctgtcatgga cacagccaca      180

gcaacagagc aggcaaagca gctggtgaag tcaggagcca tcagtgccat caaggctgag      240

accaagaact caggcttcaa gcgttctcga acccttgagg ggaagttaaa ggaccccgag      300

aagggaccag tccccacttt ccagccgttc cagaggagca tatctgctga tgatgacctg      360

caagagtcat ccagacgtcc ccagaggaaa tctctgtatg agagctttgt gtcttctagt      420

gatcgacttc gagaactagg accagatgga gaagaggcag agggcccagg ggctggtgat      480

ggtccccctc gaagctttga ctggggctat gaagaacgca gtggtgccca ctcctcagcc      540

tcccctcccc gaagccgcag ccgggaccgc agccatgaga ggaaccggga cagagaccga      600

gatcgggagc gggatcgaga ccgggatcga gacagagaca gagagcggga cagggatcgg      660

gatcgggatc gagatcgaga ccgggaacgg gacagggatc gggagcggga tcgagaccga      720

gaccgagagg gtcctttccg caggtcggat tcattccctg aacggcgagc ccctaggaaa      780

gggaatactc tctatgtata tggagaagac atgacaccca cccttctccg tggggccttc      840

tctccttttg gaaacatcat tgacctctcc atggacccac ccagaaactg tgccttcgtc      900

acctatgaaa agatggagtc agcagatcag gccgttgctg agctcaacgg gacccaggtg      960

gagtctgtac agctcaaagt caacatagcc cgaaaacagc ccatgctgga tgccgctact     1020

ggcaagtctg tctggggctc cctcgctgtc cagaacagcc ctaagggttg ccaccgggac     1080

aagaggaccc agattgtcta cagtgatgac gtctacaagg aaaaccttgt ggatggcttc     1140

tag                                                                   1143


<210>  10
<211>  380
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NELFE amino acid sequence

<400>  10

Met Leu Val Ile Pro Pro Gly Leu Ser Glu Glu Glu Glu Ala Leu Gln 
1               5                   10                  15      


Lys Lys Phe Asn Lys Leu Lys Lys Lys Lys Lys Ala Leu Leu Ala Leu 
            20                  25                  30          


Lys Lys Gln Ser Ser Ser Ser Thr Thr Ser Gln Gly Gly Val Lys Arg 
        35                  40                  45              


Ser Leu Ser Glu Gln Pro Val Met Asp Thr Ala Thr Ala Thr Glu Gln 
    50                  55                  60                  


Ala Lys Gln Leu Val Lys Ser Gly Ala Ile Ser Ala Ile Lys Ala Glu 
65                  70                  75                  80  


Thr Lys Asn Ser Gly Phe Lys Arg Ser Arg Thr Leu Glu Gly Lys Leu 
                85                  90                  95      


Lys Asp Pro Glu Lys Gly Pro Val Pro Thr Phe Gln Pro Phe Gln Arg 
            100                 105                 110         


Ser Ile Ser Ala Asp Asp Asp Leu Gln Glu Ser Ser Arg Arg Pro Gln 
        115                 120                 125             


Arg Lys Ser Leu Tyr Glu Ser Phe Val Ser Ser Ser Asp Arg Leu Arg 
    130                 135                 140                 


Glu Leu Gly Pro Asp Gly Glu Glu Ala Glu Gly Pro Gly Ala Gly Asp 
145                 150                 155                 160 


Gly Pro Pro Arg Ser Phe Asp Trp Gly Tyr Glu Glu Arg Ser Gly Ala 
                165                 170                 175     


His Ser Ser Ala Ser Pro Pro Arg Ser Arg Ser Arg Asp Arg Ser His 
            180                 185                 190         


Glu Arg Asn Arg Asp Arg Asp Arg Asp Arg Glu Arg Asp Arg Asp Arg 
        195                 200                 205             


Asp Arg Asp Arg Asp Arg Glu Arg Asp Arg Asp Arg Asp Arg Asp Arg 
    210                 215                 220                 


Asp Arg Asp Arg Glu Arg Asp Arg Asp Arg Glu Arg Asp Arg Asp Arg 
225                 230                 235                 240 


Asp Arg Glu Gly Pro Phe Arg Arg Ser Asp Ser Phe Pro Glu Arg Arg 
                245                 250                 255     


Ala Pro Arg Lys Gly Asn Thr Leu Tyr Val Tyr Gly Glu Asp Met Thr 
            260                 265                 270         


Pro Thr Leu Leu Arg Gly Ala Phe Ser Pro Phe Gly Asn Ile Ile Asp 
        275                 280                 285             


Leu Ser Met Asp Pro Pro Arg Asn Cys Ala Phe Val Thr Tyr Glu Lys 
    290                 295                 300                 


Met Glu Ser Ala Asp Gln Ala Val Ala Glu Leu Asn Gly Thr Gln Val 
305                 310                 315                 320 


Glu Ser Val Gln Leu Lys Val Asn Ile Ala Arg Lys Gln Pro Met Leu 
                325                 330                 335     


Asp Ala Ala Thr Gly Lys Ser Val Trp Gly Ser Leu Ala Val Gln Asn 
            340                 345                 350         


Ser Pro Lys Gly Cys His Arg Asp Lys Arg Thr Gln Ile Val Tyr Ser 
        355                 360                 365             


Asp Asp Val Tyr Lys Glu Asn Leu Val Asp Gly Phe 
    370                 375                 380 


