                         SEQUENCE LISTING

<110>  Agency for Science, Technology and Research
       Tan Tock Seng Hospital
 
<120>  Serological Test for Zika Virus

<130>  S61014984

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  352
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Zika WT NS1

<400>  1

Asp Val Gly Cys Ser Val Asp Phe Ser Lys Lys Glu Thr Arg Cys Gly 
1               5                   10                  15      


Thr Gly Val Phe Val Tyr Asn Asp Val Glu Ala Trp Arg Asp Arg Tyr 
            20                  25                  30          


Lys Tyr His Pro Asp Ser Pro Arg Arg Leu Ala Ala Ala Val Lys Gln 
        35                  40                  45              


Ala Trp Glu Asp Gly Ile Cys Gly Ile Ser Ser Val Ser Arg Met Glu 
    50                  55                  60                  


Asn Ile Met Trp Arg Ser Val Glu Gly Glu Leu Asn Ala Ile Leu Glu 
65                  70                  75                  80  


Glu Asn Gly Val Gln Leu Thr Val Val Val Gly Ser Val Lys Asn Pro 
                85                  90                  95      


Met Trp Arg Gly Pro Gln Arg Leu Pro Val Pro Val Asn Glu Leu Pro 
            100                 105                 110         


His Gly Trp Lys Ala Trp Gly Lys Ser Tyr Phe Val Arg Ala Ala Lys 
        115                 120                 125             


Thr Asn Asn Ser Phe Val Val Asp Gly Asp Thr Leu Lys Glu Cys Pro 
    130                 135                 140                 


Leu Lys His Arg Ala Trp Asn Ser Phe Leu Val Glu Asp His Gly Phe 
145                 150                 155                 160 


Gly Val Phe His Thr Ser Val Trp Leu Lys Val Arg Glu Asp Tyr Ser 
                165                 170                 175     


Leu Glu Cys Asp Pro Ala Val Ile Gly Thr Ala Val Lys Gly Lys Glu 
            180                 185                 190         


Ala Val His Ser Asp Leu Gly Tyr Trp Ile Glu Ser Glu Lys Asn Asp 
        195                 200                 205             


Thr Trp Arg Leu Lys Arg Ala His Leu Ile Glu Met Lys Thr Cys Glu 
    210                 215                 220                 


Trp Pro Lys Ser His Thr Leu Trp Thr Asp Gly Ile Glu Glu Ser Asp 
225                 230                 235                 240 


Leu Ile Ile Pro Lys Ser Leu Ala Gly Pro Leu Ser His His Asn Thr 
                245                 250                 255     


Arg Glu Gly Tyr Arg Thr Gln Met Lys Gly Pro Trp His Ser Glu Glu 
            260                 265                 270         


Leu Glu Ile Arg Phe Glu Glu Cys Pro Gly Thr Lys Val His Val Glu 
        275                 280                 285             


Glu Thr Cys Gly Thr Arg Gly Pro Ser Leu Arg Ser Thr Thr Ala Ser 
    290                 295                 300                 


Gly Arg Val Ile Glu Glu Trp Cys Cys Arg Glu Cys Thr Met Pro Pro 
305                 310                 315                 320 


Leu Ser Phe Arg Ala Lys Asp Gly Cys Trp Tyr Gly Met Glu Ile Arg 
                325                 330                 335     


Pro Arg Lys Glu Pro Glu Ser Asn Leu Val Arg Ser Met Val Thr Ala 
            340                 345                 350         


<210>  2
<211>  352
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  zMut2

<400>  2

Asp Val Gly Cys Ser Val Asp Phe Ser Lys Lys Glu Thr Arg Cys Gly 
1               5                   10                  15      


Thr Gly Val Phe Val Tyr Asn Asp Val Glu Ala Trp Arg Asp Arg Tyr 
            20                  25                  30          


Lys Tyr His Pro Asp Ser Pro Arg Arg Leu Ala Ala Ala Val Lys Gln 
        35                  40                  45              


Ala Trp Glu Asp Gly Ile Cys Gly Ile Ser Ser Val Ser Arg Met Glu 
    50                  55                  60                  


Asn Ile Met Trp Arg Ser Val Glu Gly Glu Leu Asn Ala Ile Leu Glu 
65                  70                  75                  80  


Glu Asn Gly Val Gln Leu Thr Val Val Val Gly Ser Val Lys Asn Pro 
                85                  90                  95      


Met Trp Arg Gly Pro Gln Arg Leu Pro Val Pro Val Asn Glu Leu Pro 
            100                 105                 110         


His Gly Trp Lys Ala Trp Gly Lys Ser Tyr Phe Val Arg Ala Ala Lys 
        115                 120                 125             


Thr Asn Asn Ser Phe Val Val Asp Gly Asp Thr Leu Lys Glu Cys Pro 
    130                 135                 140                 


Leu Lys His Arg Ala Trp Asn Ser Phe Leu Val Glu Asp His Gly Phe 
145                 150                 155                 160 


Gly Val Phe His Thr Ser Val Trp Leu Lys Val Arg Glu Asp Tyr Ser 
                165                 170                 175     


Leu Glu Cys Asp Pro Ala Val Ile Gly Thr Ala Val Lys Gly Lys Glu 
            180                 185                 190         


Ala Val His Ser Asp Leu Gly Tyr Trp Ile Glu Ser Glu Lys Asn Asp 
        195                 200                 205             


Thr Trp Arg Leu Lys Arg Ala His Leu Ile Glu Met Lys Thr Cys Glu 
    210                 215                 220                 


Trp Pro Lys Ser His Thr Leu Trp Thr Asp Gly Ile Glu Glu Ser Asp 
225                 230                 235                 240 


Leu Ile Ile Pro Lys Ser Leu Ala Gly Pro Leu Ser His His Asn Thr 
                245                 250                 255     


Arg Glu Gly Tyr Arg Thr Gln Met Lys Gly Pro Trp His Ser Glu Glu 
            260                 265                 270         


Leu Glu Ile Arg Phe Glu Glu Cys Pro Gly Thr Lys Val His Val Glu 
        275                 280                 285             


Glu Thr Cys Gly Thr Arg Gly Pro Ser Leu Arg Ser Thr Thr Ala Ser 
    290                 295                 300                 


Gly Arg Val Ile Glu Glu Trp Cys Cys Arg Glu Cys Thr Met Pro Pro 
305                 310                 315                 320 


Leu Ser Phe Arg Ala Lys Asp Gly Cys Trp Tyr Gly Met Glu Ile Arg 
                325                 330                 335     


Pro Arg Lys Glu His Glu Ser Lys Leu Val Arg Asp Met Val His Asp 
            340                 345                 350         


<210>  3
<211>  352
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  zMut1

<400>  3

Asp Val Gly Cys Ser Val Asp Phe Ser Lys Lys Glu Thr Arg Cys Gly 
1               5                   10                  15      


Thr Gly Val Phe Val Tyr Asn Asp Val Glu Ala Trp Arg Asp Arg Tyr 
            20                  25                  30          


Lys Tyr His Pro Asp Ser Pro Arg Arg Leu Ala Ala Ala Val Lys Gln 
        35                  40                  45              


Ala Trp Glu Asp Gly Ile Cys Gly Ile Ser Ser Val Ser Arg Met Glu 
    50                  55                  60                  


Asn Ile Met Trp Arg Ser Val Glu Gly Glu Leu Asn Ala Ile Leu Glu 
65                  70                  75                  80  


Glu Asn Gly Val Gln Leu Thr Val Val Val Gly Ser Val Lys Asn Pro 
                85                  90                  95      


Met Trp Arg Gly Pro Gln Arg Leu Pro Val Pro Val Asn Glu Leu Pro 
            100                 105                 110         


His Gly Trp Lys Ala Trp Gly Lys Ser Tyr Phe Val Arg Ala Ala Lys 
        115                 120                 125             


Thr Asn Asn Ser Phe Val Val Asp Gly Asp Thr Leu Lys Glu Cys Pro 
    130                 135                 140                 


Leu Lys His Arg Ala Trp Asn Ser Phe Leu Val Glu Asp His Gly Phe 
145                 150                 155                 160 


Gly Val Phe His Thr Ser Val Trp Leu Lys Val Arg Glu Asp Tyr Ser 
                165                 170                 175     


Leu Glu Cys Asp Pro Ala Val Ile Gly Thr Ala Val Lys Gly Lys Glu 
            180                 185                 190         


Ala Val His Ser Asp Leu Gly Tyr Trp Ile Glu Ser Glu Lys Asn Asp 
        195                 200                 205             


Thr Trp Arg Leu Lys Arg Ala His Leu Ile Glu Met Lys Thr Cys Glu 
    210                 215                 220                 


Trp Pro Lys Ser His Thr Leu Trp Thr Asp Gly Ile Glu Glu Ser Asp 
225                 230                 235                 240 


Leu Ile Ile Pro Lys Ser Leu Ala Gly Pro Leu Ser His His Asn Thr 
                245                 250                 255     


Arg Glu Gly Tyr Arg Thr Gln Met Lys Gly Pro Trp His Ser Glu Glu 
            260                 265                 270         


Leu Glu Ile Arg Phe Glu Glu Cys Pro Gly Thr Lys Val His Val Glu 
        275                 280                 285             


Glu Thr Cys Gly Thr Arg Gly Pro Ser Leu Arg Ser Thr Thr Ala Ser 
    290                 295                 300                 


Gly Arg Val Ile Glu Glu Trp Cys Cys Arg Glu Cys Thr Met Pro Pro 
305                 310                 315                 320 


Leu Ser Phe Arg Ala Lys Asp Gly Cys Trp Tyr Gly Met Glu Ile Arg 
                325                 330                 335     


Pro Arg Lys Gln Pro Glu Ser Asp Leu Val Arg Ser Met Thr Thr Ala 
            340                 345                 350         


<210>  4
<211>  220
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminal soluble tag

<400>  4

His His His His His His Ala Ala Ala Asp Ala His Lys Ser Glu Val 
1               5                   10                  15      


Ala His Arg Phe Lys Asp Leu Gly Glu Glu Asn Phe Lys Ala Leu Val 
            20                  25                  30          


Leu Ile Ala Phe Ala Gln Tyr Leu Gln Gln Cys Pro Phe Glu Asp His 
        35                  40                  45              


Val Lys Leu Val Asn Glu Val Thr Glu Phe Ala Lys Thr Cys Val Ala 
    50                  55                  60                  


Asp Glu Ser Ala Glu Asn Cys Asp Lys Ser Leu His Thr Leu Phe Gly 
65                  70                  75                  80  


Asp Lys Leu Cys Thr Val Ala Thr Leu Arg Glu Thr Tyr Gly Glu Met 
                85                  90                  95      


Ala Asp Cys Cys Ala Lys Gln Glu Pro Glu Arg Asn Glu Cys Phe Leu 
            100                 105                 110         


Gln His Lys Asp Asp Asn Pro Asn Leu Pro Arg Leu Val Arg Pro Glu 
        115                 120                 125             


Val Asp Val Met Cys Thr Ala Phe His Asp Asn Glu Glu Thr Phe Leu 
    130                 135                 140                 


Lys Lys Tyr Leu Tyr Glu Ile Ala Arg Arg His Pro Tyr Phe Tyr Ala 
145                 150                 155                 160 


Pro Glu Leu Leu Phe Phe Ala Lys Arg Tyr Lys Ala Ala Phe Thr Glu 
                165                 170                 175     


Cys Cys Gln Ala Ala Asp Lys Ala Ala Cys Leu Leu Pro Lys Leu Asp 
            180                 185                 190         


Glu Leu Arg Asp Glu Gly Lys Ala Ser Ser Ala Lys Gln Gly Ala Ala 
        195                 200                 205             


Ser Ala Pro Ser Thr Gly Ser Lys Pro Thr Ala Pro 
    210                 215                 220 


<210>  5
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  JEV_M55506 (320 to 352)

<400>  5

Pro Leu Arg Phe Arg Thr Glu Asn Gly Cys Trp Tyr Gly Met Glu Ile 
1               5                   10                  15      


Arg Pro Val Met His Asp Glu Thr Thr Leu Val Arg Ser Gln Val Asp 
            20                  25                  30          


Ala 
    


<210>  6
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  WNV-AF196835 (320 to 352)

<400>  6

Pro Leu Arg Tyr Gln Thr Asp Ser Gly Cys Trp Tyr Gly Met Glu Ile 
1               5                   10                  15      


Arg Pro Gln Arg His Asp Glu Lys Thr Leu Val Gln Ser Gln Val Asn 
            20                  25                  30          


Ala 
    


<210>  7
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV-KU365779.1 (320 to 352)

<400>  7

Pro Leu Ser Phe Arg Ala Lys Asp Gly Cys Trp Tyr Gly Met Glu Ile 
1               5                   10                  15      


Arg Pro Arg Lys Glu Pro Glu Ser Asn Leu Val Arg Ser Met Val Thr 
            20                  25                  30          


Ala 
    


<210>  8
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV-AY632535

<400>  8

Pro Leu Ser Phe Arg Ala Lys Asp Gly Cys Trp Tyr Gly Met Glu Ile 
1               5                   10                  15      


Arg Pro Arg Lys Glu Pro Glu Ser Asn Leu Val Arg Ser Met Val Thr 
            20                  25                  30          


Ala 
    


<210>  9
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DENV1_U88535

<400>  9

Pro Leu Arg Phe Lys Gly Glu Asp Gly Cys Trp Tyr Gly Met Glu Ile 
1               5                   10                  15      


Arg Pro Val Lys Glu Lys Glu Glu Asn Leu Val Lys Ser Met Val Ser 
            20                  25                  30          


Ala 
    


<210>  10
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DENV2_M84727

<400>  10

Pro Leu Arg Tyr Arg Gly Glu Asp Gly Cys Trp Tyr Gly Met Glu Ile 
1               5                   10                  15      


Arg Pro Leu Lys Glu Lys Glu Glu Asn Leu Val Asn Ser Leu Val Thr 
            20                  25                  30          


Ala 
    


<210>  11
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DENV3-EF643017

<400>  11

Pro Leu Arg Tyr Met Gly Glu Asp Gly Cys Trp Tyr Gly Met Glu Ile 
1               5                   10                  15      


Arg Pro Ile Ser Glu Lys Glu Glu Asn Met Val Lys Ser Leu Val Ser 
            20                  25                  30          


Ala 
    


<210>  12
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DENV4-AF326573

<400>  12

Pro Leu Arg Phe Leu Gly Glu Asp Gly Cys Trp Tyr Gly Met Glu Ile 
1               5                   10                  15      


Arg Pro Leu Ser Glu Lys Glu Glu Asn Met Val Lys Ser Gln Val Thr 
            20                  25                  30          


Ala 
    


