                         SEQUENCE LISTING

<110>  Buehlmann Laboratories AG
 
<120>  Recombinant Protein

<130>  38366-BHL-P-WO

<160>  19    

<170>  PatentIn version 3.5

<210>  1
<211>  93
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  Human S100A8/Mrp8 protein sequence

<400>  1

Met Leu Thr Glu Leu Glu Lys Ala Leu Asn Ser Ile Ile Asp Val Tyr 
1               5                   10                  15      


His Lys Tyr Ser Leu Ile Lys Gly Asn Phe His Ala Val Tyr Arg Asp 
            20                  25                  30          


Asp Leu Lys Lys Leu Leu Glu Thr Glu Cys Pro Gln Tyr Ile Arg Lys 
        35                  40                  45              


Lys Gly Ala Asp Val Trp Phe Lys Glu Leu Asp Ile Asn Thr Asp Gly 
    50                  55                  60                  


Ala Val Asn Phe Gln Glu Phe Leu Ile Leu Val Ile Lys Met Gly Val 
65                  70                  75                  80  


Ala Ala His Lys Lys Ser His Glu Glu Ser His Lys Glu 
                85                  90              


<210>  2
<211>  114
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  Human S100A9/Mrp14 protein sequence

<400>  2

Met Thr Cys Lys Met Ser Gln Leu Glu Arg Asn Ile Glu Thr Ile Ile 
1               5                   10                  15      


Asn Thr Phe His Gln Tyr Ser Val Lys Leu Gly His Pro Asp Thr Leu 
            20                  25                  30          


Asn Gln Gly Glu Phe Lys Glu Leu Val Arg Lys Asp Leu Gln Asn Phe 
        35                  40                  45              


Leu Lys Lys Glu Asn Lys Asn Glu Lys Val Ile Glu His Ile Met Glu 
    50                  55                  60                  


Asp Leu Asp Thr Asn Ala Asp Lys Gln Leu Ser Phe Glu Glu Phe Ile 
65                  70                  75                  80  


Met Leu Met Ala Arg Leu Thr Trp Ala Ser His Glu Lys Met His Glu 
                85                  90                  95      


Gly Asp Glu Gly Pro Gly His His His Lys Pro Gly Leu Gly Glu Gly 
            100                 105                 110         


Thr Pro 
        


<210>  3
<211>  230
<212>  PRT
<213>  Artificial

<220>
<223>  Sequence of HisTag-3C Protease Seq.-MRP14-3C Protease 
       Seq.[Linker]-MRP8

<400>  3

Met His His His His His His Leu Glu Val Leu Phe Gln Gly Pro Met 
1               5                   10                  15      


Thr Cys Lys Met Ser Gln Leu Glu Arg Asn Ile Glu Thr Ile Ile Asn 
            20                  25                  30          


Thr Phe His Gln Tyr Ser Val Lys Leu Gly His Pro Asp Thr Leu Asn 
        35                  40                  45              


Gln Gly Glu Phe Lys Glu Leu Val Arg Lys Asp Leu Gln Asn Phe Leu 
    50                  55                  60                  


Lys Lys Glu Asn Lys Asn Glu Lys Val Ile Glu His Ile Met Glu Asp 
65                  70                  75                  80  


Leu Asp Thr Asn Ala Asp Lys Gln Leu Ser Phe Glu Glu Phe Ile Met 
                85                  90                  95      


Leu Met Ala Arg Leu Thr Trp Ala Ser His Glu Lys Met His Glu Gly 
            100                 105                 110         


Asp Glu Gly Pro Gly His His His Lys Pro Gly Leu Gly Glu Gly Thr 
        115                 120                 125             


Pro Leu Glu Val Leu Phe Gln Gly Pro Met Leu Thr Glu Leu Glu Lys 
    130                 135                 140                 


Ala Leu Asn Ser Ile Ile Asp Val Tyr His Lys Tyr Ser Leu Ile Lys 
145                 150                 155                 160 


Gly Asn Phe His Ala Val Tyr Arg Asp Asp Leu Lys Lys Leu Leu Glu 
                165                 170                 175     


Thr Glu Cys Pro Gln Tyr Ile Arg Lys Lys Gly Ala Asp Val Trp Phe 
            180                 185                 190         


Lys Glu Leu Asp Ile Asn Thr Asp Gly Ala Val Asn Phe Gln Glu Phe 
        195                 200                 205             


Leu Ile Leu Val Ile Lys Met Gly Val Ala Ala His Lys Lys Ser His 
    210                 215                 220                 


Glu Glu Ser His Lys Glu 
225                 230 


<210>  4
<211>  229
<212>  PRT
<213>  Artificial

<220>
<223>  MRP8-3C Protease Seq.[Linker]-MRP14-3C Protease Seq.-HisTag

<400>  4

Met Leu Thr Glu Leu Glu Lys Ala Leu Asn Ser Ile Ile Asp Val Tyr 
1               5                   10                  15      


His Lys Tyr Ser Leu Ile Lys Gly Asn Phe His Ala Val Tyr Arg Asp 
            20                  25                  30          


Asp Leu Lys Lys Leu Leu Glu Thr Glu Cys Pro Gln Tyr Ile Arg Lys 
        35                  40                  45              


Lys Gly Ala Asp Val Trp Phe Lys Glu Leu Asp Ile Asn Thr Asp Gly 
    50                  55                  60                  


Ala Val Asn Phe Gln Glu Phe Leu Ile Leu Val Ile Lys Met Gly Val 
65                  70                  75                  80  


Ala Ala His Lys Lys Ser His Glu Glu Ser His Lys Glu Leu Glu Val 
                85                  90                  95      


Leu Phe Gln Gly Pro Met Thr Cys Lys Met Ser Gln Leu Glu Arg Asn 
            100                 105                 110         


Ile Glu Thr Ile Ile Asn Thr Phe His Gln Tyr Ser Val Lys Leu Gly 
        115                 120                 125             


His Pro Asp Thr Leu Asn Gln Gly Glu Phe Lys Glu Leu Val Arg Lys 
    130                 135                 140                 


Asp Leu Gln Asn Phe Leu Lys Lys Glu Asn Lys Asn Glu Lys Val Ile 
145                 150                 155                 160 


Glu His Ile Met Glu Asp Leu Asp Thr Asn Ala Asp Lys Gln Leu Ser 
                165                 170                 175     


Phe Glu Glu Phe Ile Met Leu Met Ala Arg Leu Thr Trp Ala Ser His 
            180                 185                 190         


Glu Lys Met His Glu Gly Asp Glu Gly Pro Gly His His His Lys Pro 
        195                 200                 205             


Gly Leu Gly Glu Gly Thr Pro Leu Glu Val Leu Phe Gln Gly Pro His 
    210                 215                 220                 


His His His His His 
225                 


<210>  5
<211>  693
<212>  DNA
<213>  Artificial

<220>
<223>  DNA sequence of HisTag-3C Protease Seq.-MRP14-3C Protease 
       Seq.[Linker]-MRP8 for expression in E.coli

<400>  5
atgcaccatc atcaccatca cttggaagtt ctgtttcaag gcccaatgac gtgcaaaatg       60

agccagttgg aacgcaatat tgaaaccatc attaacacct ttcatcagta ttccgtcaaa      120

ctgggtcatc cggataccct taatcaaggg gaatttaaag agctggtacg caaagatctc      180

cagaatttcc tgaagaaaga aaacaagaac gagaaagtca tcgaacacat tatggaagat      240

ctggatacca acgcggataa acagttatcg tttgaggagt tcattatgct gatggcacgt      300

ctgacatggg cctcacatga gaaaatgcat gaaggcgatg agggtcctgg acatcatcac      360

aaaccggggt taggcgaagg aactccgtta gaagtgttgt ttcaaggtcc gatgctgacg      420

gaactggaga aagcgcttaa cagcattatt gacgtttacc acaaatacag tctgatcaaa      480

ggcaatttcc atgcggtata tcgtgacgat ctgaagaaac ttctcgaaac ggaatgtccc      540

cagtatatcc gcaagaaagg tgccgatgtg tggttcaaag aactggacat taacactgac      600

ggtgcagtga atttccagga atttctcatt ctggtgatca aaatgggcgt tgctgctcac      660

aagaaatcgc atgaagagtc tcacaaagaa taa                                   693


<210>  6
<211>  690
<212>  DNA
<213>  Artificial

<220>
<223>  DNA sequence for expression of MRP8-3C Protease 
       Seq.[Linker]-MRP14-3C Protease Seq.-HisTag in E.coli

<400>  6
atgctgacgg aactggagaa agcgcttaac agcattattg acgtttacca caaatacagt       60

ctgatcaaag gcaatttcca tgcggtatat cgtgacgatc tgaagaaact tctcgaaacg      120

gaatgtcccc agtatatccg caagaaaggt gccgatgtgt ggttcaaaga actggacatt      180

aacactgacg gtgcagtgaa tttccaggaa tttctcattc tggtgatcaa aatgggcgtt      240

gctgctcaca agaaatcgca tgaagagtct cacaaagaat tggaagttct gtttcaaggc      300

ccaatgacgt gcaaaatgag ccagttggaa cgcaatattg aaaccatcat taacaccttt      360

catcagtatt ccgtcaaact gggtcatccg gataccctta atcaagggga atttaaagag      420

ctggtacgca aagatctcca gaatttcctg aagaaagaaa acaagaacga gaaagtcatc      480

gaacacatta tggaagatct ggataccaac gcggataaac agttatcgtt tgaggagttc      540

attatgctga tggcacgtct gacatgggcc tcacatgaga aaatgcatga aggcgatgag      600

ggtcctggac atcatcacaa accggggtta ggcgaaggaa ctccgttgga agttctgttt      660

caaggcccac accatcatca ccatcactaa                                       690


<210>  7
<211>  229
<212>  PRT
<213>  Artificial

<220>
<223>  HisTag-3C Protease Seq.-MRP14-TEV Protease seq.[Linker]-MRP8

<400>  7

Met His His His His His His Leu Glu Val Leu Phe Gln Gly Pro Met 
1               5                   10                  15      


Thr Cys Lys Met Ser Gln Leu Glu Arg Asn Ile Glu Thr Ile Ile Asn 
            20                  25                  30          


Thr Phe His Gln Tyr Ser Val Lys Leu Gly His Pro Asp Thr Leu Asn 
        35                  40                  45              


Gln Gly Glu Phe Lys Glu Leu Val Arg Lys Asp Leu Gln Asn Phe Leu 
    50                  55                  60                  


Lys Lys Glu Asn Lys Asn Glu Lys Val Ile Glu His Ile Met Glu Asp 
65                  70                  75                  80  


Leu Asp Thr Asn Ala Asp Lys Gln Leu Ser Phe Glu Glu Phe Ile Met 
                85                  90                  95      


Leu Met Ala Arg Leu Thr Trp Ala Ser His Glu Lys Met His Glu Gly 
            100                 105                 110         


Asp Glu Gly Pro Gly His His His Lys Pro Gly Leu Gly Glu Gly Thr 
        115                 120                 125             


Pro Glu Asn Leu Tyr Phe Gln Ser Met Leu Thr Glu Leu Glu Lys Ala 
    130                 135                 140                 


Leu Asn Ser Ile Ile Asp Val Tyr His Lys Tyr Ser Leu Ile Lys Gly 
145                 150                 155                 160 


Asn Phe His Ala Val Tyr Arg Asp Asp Leu Lys Lys Leu Leu Glu Thr 
                165                 170                 175     


Glu Cys Pro Gln Tyr Ile Arg Lys Lys Gly Ala Asp Val Trp Phe Lys 
            180                 185                 190         


Glu Leu Asp Ile Asn Thr Asp Gly Ala Val Asn Phe Gln Glu Phe Leu 
        195                 200                 205             


Ile Leu Val Ile Lys Met Gly Val Ala Ala His Lys Lys Ser His Glu 
    210                 215                 220                 


Glu Ser His Lys Glu 
225                 


<210>  8
<211>  229
<212>  PRT
<213>  Artificial

<220>
<223>  HisTag-3C Protease Seq.-MRP8-TEV Protease seq.[Linker]-MRP14

<400>  8

Met His His His His His His Leu Glu Val Leu Phe Gln Gly Pro Met 
1               5                   10                  15      


Leu Thr Glu Leu Glu Lys Ala Leu Asn Ser Ile Ile Asp Val Tyr His 
            20                  25                  30          


Lys Tyr Ser Leu Ile Lys Gly Asn Phe His Ala Val Tyr Arg Asp Asp 
        35                  40                  45              


Leu Lys Lys Leu Leu Glu Thr Glu Cys Pro Gln Tyr Ile Arg Lys Lys 
    50                  55                  60                  


Gly Ala Asp Val Trp Phe Lys Glu Leu Asp Ile Asn Thr Asp Gly Ala 
65                  70                  75                  80  


Val Asn Phe Gln Glu Phe Leu Ile Leu Val Ile Lys Met Gly Val Ala 
                85                  90                  95      


Ala His Lys Lys Ser His Glu Glu Ser His Lys Glu Glu Asn Leu Tyr 
            100                 105                 110         


Phe Gln Ser Met Thr Cys Lys Met Ser Gln Leu Glu Arg Asn Ile Glu 
        115                 120                 125             


Thr Ile Ile Asn Thr Phe His Gln Tyr Ser Val Lys Leu Gly His Pro 
    130                 135                 140                 


Asp Thr Leu Asn Gln Gly Glu Phe Lys Glu Leu Val Arg Lys Asp Leu 
145                 150                 155                 160 


Gln Asn Phe Leu Lys Lys Glu Asn Lys Asn Glu Lys Val Ile Glu His 
                165                 170                 175     


Ile Met Glu Asp Leu Asp Thr Asn Ala Asp Lys Gln Leu Ser Phe Glu 
            180                 185                 190         


Glu Phe Ile Met Leu Met Ala Arg Leu Thr Trp Ala Ser His Glu Lys 
        195                 200                 205             


Met His Glu Gly Asp Glu Gly Pro Gly His His His Lys Pro Gly Leu 
    210                 215                 220                 


Gly Glu Gly Thr Pro 
225                 


<210>  9
<211>  229
<212>  PRT
<213>  Artificial

<220>
<223>  HisTag-TEV Protease seq.-MRP14-3C Protease Seq. [Linker]-MRP8

<400>  9

Met His His His His His His Glu Asn Leu Tyr Phe Gln Ser Met Thr 
1               5                   10                  15      


Cys Lys Met Ser Gln Leu Glu Arg Asn Ile Glu Thr Ile Ile Asn Thr 
            20                  25                  30          


Phe His Gln Tyr Ser Val Lys Leu Gly His Pro Asp Thr Leu Asn Gln 
        35                  40                  45              


Gly Glu Phe Lys Glu Leu Val Arg Lys Asp Leu Gln Asn Phe Leu Lys 
    50                  55                  60                  


Lys Glu Asn Lys Asn Glu Lys Val Ile Glu His Ile Met Glu Asp Leu 
65                  70                  75                  80  


Asp Thr Asn Ala Asp Lys Gln Leu Ser Phe Glu Glu Phe Ile Met Leu 
                85                  90                  95      


Met Ala Arg Leu Thr Trp Ala Ser His Glu Lys Met His Glu Gly Asp 
            100                 105                 110         


Glu Gly Pro Gly His His His Lys Pro Gly Leu Gly Glu Gly Thr Pro 
        115                 120                 125             


Leu Glu Val Leu Phe Gln Gly Pro Met Leu Thr Glu Leu Glu Lys Ala 
    130                 135                 140                 


Leu Asn Ser Ile Ile Asp Val Tyr His Lys Tyr Ser Leu Ile Lys Gly 
145                 150                 155                 160 


Asn Phe His Ala Val Tyr Arg Asp Asp Leu Lys Lys Leu Leu Glu Thr 
                165                 170                 175     


Glu Cys Pro Gln Tyr Ile Arg Lys Lys Gly Ala Asp Val Trp Phe Lys 
            180                 185                 190         


Glu Leu Asp Ile Asn Thr Asp Gly Ala Val Asn Phe Gln Glu Phe Leu 
        195                 200                 205             


Ile Leu Val Ile Lys Met Gly Val Ala Ala His Lys Lys Ser His Glu 
    210                 215                 220                 


Glu Ser His Lys Glu 
225                 


<210>  10
<211>  229
<212>  PRT
<213>  Artificial

<220>
<223>  HisTag-TEV Protease seq.-MRP8-3C Protease Seq. [Linker]-MRP14

<400>  10

Met His His His His His His Glu Asn Leu Tyr Phe Gln Ser Met Leu 
1               5                   10                  15      


Thr Glu Leu Glu Lys Ala Leu Asn Ser Ile Ile Asp Val Tyr His Lys 
            20                  25                  30          


Tyr Ser Leu Ile Lys Gly Asn Phe His Ala Val Tyr Arg Asp Asp Leu 
        35                  40                  45              


Lys Lys Leu Leu Glu Thr Glu Cys Pro Gln Tyr Ile Arg Lys Lys Gly 
    50                  55                  60                  


Ala Asp Val Trp Phe Lys Glu Leu Asp Ile Asn Thr Asp Gly Ala Val 
65                  70                  75                  80  


Asn Phe Gln Glu Phe Leu Ile Leu Val Ile Lys Met Gly Val Ala Ala 
                85                  90                  95      


His Lys Lys Ser His Glu Glu Ser His Lys Glu Leu Glu Val Leu Phe 
            100                 105                 110         


Gln Gly Pro Met Thr Cys Lys Met Ser Gln Leu Glu Arg Asn Ile Glu 
        115                 120                 125             


Thr Ile Ile Asn Thr Phe His Gln Tyr Ser Val Lys Leu Gly His Pro 
    130                 135                 140                 


Asp Thr Leu Asn Gln Gly Glu Phe Lys Glu Leu Val Arg Lys Asp Leu 
145                 150                 155                 160 


Gln Asn Phe Leu Lys Lys Glu Asn Lys Asn Glu Lys Val Ile Glu His 
                165                 170                 175     


Ile Met Glu Asp Leu Asp Thr Asn Ala Asp Lys Gln Leu Ser Phe Glu 
            180                 185                 190         


Glu Phe Ile Met Leu Met Ala Arg Leu Thr Trp Ala Ser His Glu Lys 
        195                 200                 205             


Met His Glu Gly Asp Glu Gly Pro Gly His His His Lys Pro Gly Leu 
    210                 215                 220                 


Gly Glu Gly Thr Pro 
225                 


<210>  11
<211>  282
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Human S100A8/Mrp8 DNA sequence

<400>  11
atgttgaccg agctggagaa agccttgaac tctatcatcg acgtctacca caagtactcc       60

ctgataaagg ggaatttcca tgccgtctac agggatgacc tgaagaaatt gctagagacc      120

gagtgtcctc agtatatcag gaaaaagggt gcagacgtct ggttcaaaga gttggatatc      180

aacactgatg gtgcagttaa cttccaggag ttcctcattc tggtgataaa gatgggcgtg      240

gcagcccaca aaaaaagcca tgaagaaagc cacaaagagt ag                         282


<210>  12
<211>  282
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Human S100A9/Mrp14 DNA sequence

<400>  12
atgttgaccg agctggagaa agccttgaac tctatcatcg acgtctacca caagtactcc       60

ctgataaagg ggaatttcca tgccgtctac agggatgacc tgaagaaatt gctagagacc      120

gagtgtcctc agtatatcag gaaaaagggt gcagacgtct ggttcaaaga gttggatatc      180

aacactgatg gtgcagttaa cttccaggag ttcctcattc tggtgataaa gatgggcgtg      240

gcagcccaca aaaaaagcca tgaagaaagc cacaaagagt ag                         282


<210>  13
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  Recognition sequence for Rhinovirus 3C Protease (Prescission 
       protease)

<400>  13

Leu Glu Val Leu Phe Gln Gly Pro 
1               5               


<210>  14
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Recognition sequence for TEV protease

<400>  14

Glu Asn Leu Tyr Phe Gln Gly 
1               5           


<210>  15
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  Recognition sequence for thrombin

<400>  15

Leu Val Pro Arg Gly Ser 
1               5       


<210>  16
<211>  4
<212>  PRT
<213>  Artificial

<220>
<223>  Recognition sequence for factor Xa; X is E or D


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  D or E

<400>  16

Ile Xaa Gly Arg 
1               


<210>  17
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Recognition sequence for an enteropeptidase

<400>  17

Asp Asp Asp Asp Lys 
1               5   


<210>  18
<211>  254
<212>  PRT
<213>  Artificial

<220>
<223>  HisTag-3C Protease Seq.-MRP14-3C Protease Seq.[Linker]-MRP8 
       (Isoform a)

<400>  18

Met His His His His His His Leu Glu Val Leu Phe Gln Gly Pro Met 
1               5                   10                  15      


Thr Cys Lys Met Ser Gln Leu Glu Arg Asn Ile Glu Thr Ile Ile Asn 
            20                  25                  30          


Thr Phe His Gln Tyr Ser Val Lys Leu Gly His Pro Asp Thr Leu Asn 
        35                  40                  45              


Gln Gly Glu Phe Lys Glu Leu Val Arg Lys Asp Leu Gln Asn Phe Leu 
    50                  55                  60                  


Lys Lys Glu Asn Lys Asn Glu Lys Val Ile Glu His Ile Met Glu Asp 
65                  70                  75                  80  


Leu Asp Thr Asn Ala Asp Lys Gln Leu Ser Phe Glu Glu Phe Ile Met 
                85                  90                  95      


Leu Met Ala Arg Leu Thr Trp Ala Ser His Glu Lys Met His Glu Gly 
            100                 105                 110         


Asp Glu Gly Pro Gly His His His Lys Pro Gly Leu Gly Glu Gly Thr 
        115                 120                 125             


Pro Leu Glu Val Leu Phe Gln Gly Pro Met Ser Leu Val Ser Cys Leu 
    130                 135                 140                 


Ser Glu Asp Leu Lys Val Leu Phe Phe Arg Trp Gly Lys Ser Val Gly 
145                 150                 155                 160 


Ile Met Leu Thr Glu Leu Glu Lys Ala Leu Asn Ser Ile Ile Asp Val 
                165                 170                 175     


Tyr His Lys Tyr Ser Leu Ile Lys Gly Asn Phe His Ala Val Tyr Arg 
            180                 185                 190         


Asp Asp Leu Lys Lys Leu Leu Glu Thr Glu Cys Pro Gln Tyr Ile Arg 
        195                 200                 205             


Lys Lys Gly Ala Asp Val Trp Phe Lys Glu Leu Asp Ile Asn Thr Asp 
    210                 215                 220                 


Gly Ala Val Asn Phe Gln Glu Phe Leu Ile Leu Val Ile Lys Met Gly 
225                 230                 235                 240 


Val Ala Ala His Lys Lys Ser His Glu Glu Ser His Lys Glu 
                245                 250                 


<210>  19
<211>  254
<212>  PRT
<213>  Artificial sequence

<220>
<223>  HisTag-3C Protease Seq.- MRP8 (Isoform a)-3C Protease 
       Seq.[Linker]- MRP14

<400>  19

Met His His His His His His Leu Glu Val Leu Phe Gln Gly Pro Met 
1               5                   10                  15      


Ser Leu Val Ser Cys Leu Ser Glu Asp Leu Lys Val Leu Phe Phe Arg 
            20                  25                  30          


Trp Gly Lys Ser Val Gly Ile Met Leu Thr Glu Leu Glu Lys Ala Leu 
        35                  40                  45              


Asn Ser Ile Ile Asp Val Tyr His Lys Tyr Ser Leu Ile Lys Gly Asn 
    50                  55                  60                  


Phe His Ala Val Tyr Arg Asp Asp Leu Lys Lys Leu Leu Glu Thr Glu 
65                  70                  75                  80  


Cys Pro Gln Tyr Ile Arg Lys Lys Gly Ala Asp Val Trp Phe Lys Glu 
                85                  90                  95      


Leu Asp Ile Asn Thr Asp Gly Ala Val Asn Phe Gln Glu Phe Leu Ile 
            100                 105                 110         


Leu Val Ile Lys Met Gly Val Ala Ala His Lys Lys Ser His Glu Glu 
        115                 120                 125             


Ser His Lys Glu Leu Glu Val Leu Phe Gln Gly Pro Met Thr Cys Lys 
    130                 135                 140                 


Met Ser Gln Leu Glu Arg Asn Ile Glu Thr Ile Ile Asn Thr Phe His 
145                 150                 155                 160 


Gln Tyr Ser Val Lys Leu Gly His Pro Asp Thr Leu Asn Gln Gly Glu 
                165                 170                 175     


Phe Lys Glu Leu Val Arg Lys Asp Leu Gln Asn Phe Leu Lys Lys Glu 
            180                 185                 190         


Asn Lys Asn Glu Lys Val Ile Glu His Ile Met Glu Asp Leu Asp Thr 
        195                 200                 205             


Asn Ala Asp Lys Gln Leu Ser Phe Glu Glu Phe Ile Met Leu Met Ala 
    210                 215                 220                 


Arg Leu Thr Trp Ala Ser His Glu Lys Met His Glu Gly Asp Glu Gly 
225                 230                 235                 240 


Pro Gly His His His Lys Pro Gly Leu Gly Glu Gly Thr Pro 
                245                 250                 


