                         SEQUENCE LISTING

<110>  Moligo Technologies AB
 
<120>  Method and products for producing single stranded DNA 
       polynucleotides

<130>  20.11.145698/01

<150>  GB2002547.4
<151>  2020-02-24

<160>  32    

<170>  PatentIn version 3.5

<210>  1
<211>  3509
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pM1 plasmid

<400>  1
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagagaatt cacattaccc tgttatccct      420

agatgacatt accctgttat cccagatgac attaccctgt tatccctaga tgacattacc      480

ctgttatccc tagatgacat ttaccctgtt atccctagat gacattaccc tgttatccca      540

gatgacatta ccctgttatc cctagataca ttaccctgtt atcccagatg acataccctg      600

ttatccctag atgacattac cctgttatcc cagatgacat taccctgtta tccctagata      660

cattaccctg ttatcccaga tgacataccc tgttatccct agatgacatt accctgttat      720

cccagatgac attaccctgt tatccctaga tacattaccc tgttatccca gatgacatac      780

cctgttatcc ctagatgaca ttaccctgtt atcccagatg acattaccct gttatcccta      840

gatacattac cctgttatcc cagatgacat accctgttat ccctagatga cattaccctg      900

ttatcccaga tgacattacc ctgttatccc tagatacatt accctgttat cccagatgac      960

ataccctgtt atccctagat gacattaccc tgttatccca gatgacatta ccctgttatc     1020

cctagataca ttaccctgtt atcccagatg acataccctg ttatccctag atgacattac     1080

cctgttatcc cagataaact caatgatgat gatgatgatg gctcttcaaa gcaatgagtc     1140

gagactcagc ggccgcggtg ccagggcgtg cccttgggct ccccgggcgc gactatatct     1200

agatatcgga tcccgggccc gtcgactgca gaggcctgca tgcaagcttg gggcccgccc     1260

caactggggt aacctttgag ttctctcagt tgggggtaat cagcatcatg atgtggtacc     1320

acatcatgat gctgattata agaatgcggc cgccacactc tagtggatct cgagttaata     1380

attcgctctt caaagcaatg aaagcttggt gtaatcatgg tcatagctgt ttcctgtgtg     1440

aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc     1500

ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt     1560

ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg     1620

cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt     1680

tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc     1740

aggggataac gcaggaaaga catgtgagca aaaggccagc aaaaggccag gaaccgtaaa     1800

aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat     1860

cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc     1920

cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc     1980

gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt     2040

tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac     2100

cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg     2160

ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca     2220

gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc     2280

gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa     2340

accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa     2400

ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac     2460

tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta     2520

aattaaaaat gaagttttaa atcaagccca atctgaataa tgttacaacc aattaaccaa     2580

ttctgattag aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt     2640

atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca     2700

gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat     2760

acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt     2820

gacgactgaa tccggtgaga atggcaaaag tttatgcatt tctttccaga cttgttcaac     2880

aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg     2940

tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tacaaacagg     3000

aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc     3060

aggatattct tctaatacct ggaatgctgt ttttccgggg atcgcagtgg tgagtaacca     3120

tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag     3180

ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt     3240

cagaaacaac tctggcgcat cgggcttccc atacaagcga tagattgtcg cacctgattg     3300

cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa     3360

tcgcggcctc gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat     3420

gtaagcagac agttttattg ttcatgatga tatattttta tcttgtgcaa tgtaacatca     3480

gagattttga gacacgggcc agagctgca                                       3509


<210>  2
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Junction sequence

<400>  2
gggtaacctt tgggctcccc                                                   20


<210>  3
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RCA primer

<400>  3
gtaacctttg ggctc                                                        15


<210>  4
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  attP attachment site

<400>  4
ggtgccaggg cgtgcccttg ggctccccgg gcgcg                                  35


<210>  5
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  attB attachment site

<400>  5
ggtgccaggg cgtgcccttg ggctccccgg gcgcg                                  35


<210>  6
<211>  1177
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ActEVEN pseudogene

<400>  6
ggtctcacat tgcataatct tcatccgtgg gaaccacgga tgaagaaccg tcccaagcgt       60

tgcgccacat ctgctggaag gtggacagtg agaggacacc tacgaatcgc aacgggtatc      120

ctcatccgtg ggaaccacgg atgaggaacc gtcccaagcg ttgcgcctgg gtacatggtg      180

gtaccaccag acaggacacc tacgaatcgc aacgggtatc ctcatccgtg ggaaccacgg      240

atgaggaacc gtcccaagcg ttgcggagag catagccctc gtagatgggc aaggacacct      300

acgaatcgca acgggtatcc tcatccgtgg gaaccacgga tgaggaaccg tcccaagcgt      360

tgcggtccca gttggtaaca atgccatgtt caatgaggac acctacgaat cgcaacgggt      420

atcctcatcc gtgggaacca cggatgagga accgtcccaa gcgttgcgcg gactcatcgt      480

actcctgctt gctgaggaca cctacgaatc gcaacgggta tcctcatccg tgggaaccac      540

ggatgaggaa ccgtcccaag cgttgcgttc tctttgatgt cacgcacgat ttcccaggac      600

acctacgaat cgcaacgggt atcctcatcc gtgggaacca cggatgagga accgtcccaa      660

gcgttgcgct cggtcaggat cttcatgagg tagtctgtag gacacctacg aatcgcaacg      720

ggtatcctca tccgtgggaa ccacggatga ggaaccgtcc caagcgttgc gtttcacggt      780

tggccttagg gttcagggga ggacacctac gaatcgcaac gggtatcctc atccgtggga      840

accacggatg aggaaccgtc ccaagcgttg cggtacttca gggtcaggat acctctcttg      900

aggacaccta cgaatcgcaa cgggtatcct catccgtggg aaccacggat gaggaaccgt      960

cccaagcgtt gcgctgctcg aagtctagag caacatagca caaggacacc tacgaatcgc     1020

aacgggtatc ctcatccgtg ggaaccacgg atgaggaacc gtcccaagcg ttgcgcctcg     1080

tcacccacat aggagtcctt caggacacct acgaatcgca acgggtatcc tcatccgtgg     1140

gaaccacgga tgaggagctc ttcatgcatt ggagacc                              1177


<210>  7
<211>  1406
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CRC1 pseudogene

<400>  7
ggtctcacat tgcataaaat tcatccgtgg gaaccacgga tgaatttttc taatgtgccc       60

tatacacccc agccaagcca agatagcatc tgaccttacc catcttacat tattttgctt      120

aacctctctc caacaaagaa gttaaaactt tatagaatgc attattcctg agcaatagac      180

tttttaataa aatacctacg tcttacattc tttgttaggt cacctatgac cgtgtaaagt      240

ccaacatgcc tgttacaaga aaaaaaaaaa atccagcaca acctagagtt ataatccaat      300

ctttatttaa aaatctaatc tgccagttta cttgtacagc tcgtccatgc ccagggtgat      360

gccagcggcg gtcacaaatt ccagcagcac catgtggtcc cgcttctcgt tggggtcctt      420

gctcagcttg ctctgggtgc tcaggtagtg gttgtcgggc agcagcacgg ggccatctcc      480

gatgggggtg ttctgctggt agtggtcggc cagctgcacg gagccgtcct cgatgttgtg      540

ccgggtcttg aagttcactt tgatgccgtt tttctgcttg tcggcggtga tgtacacctt      600

gtggctgttg tagttgtact ccagcttgtg gcccaggata ttgccgtcct ctttgaagtc      660

gatgcccttc agctcgatcc ggttcacgag ggtgtcgccc tcgaacttca cttcggccct      720

ggtcttgtag ttgccgtcgt ccttaaagaa gatggtccgt tcctgcacgt agccctcggg      780

catggcgctc ttgaagaagt cgtgctgctt catatggtcg gggtatctgg cgaagcactg      840

cacgccgtag gtaaaggtgg tcacgagggt aggccaaggc acgggcagct tgccggtggt      900

gcagatgaac ttcagggtca gcttgccata tgtggcgtcg ccttcgccct cgccggacac      960

gctgaacttg tggccgttca cgtcgccgtc gagttccacc agaatgggca ccacgccggt     1020

gaacagttcc tcgcccttgc tcacgcttcc tccgcttcca cctccgcttc caccgcgttt     1080

tccaccaact cggggagctg aaactttcac aggcttcaca atcttttgct taggtgctgc     1140

ctttgtaggt gcctgtaaaa agataacaca atattactcc acaaaacttt tgttacccta     1200

ttattaccca tctctcacgt ccagattgtt tatgtagctt agatcacttt cttagtaatt     1260

taactgcatt cctaatgcca atcttttaat gtgactaccc ttaaatattt aaagcccatc     1320

tttacaatga aagacaaaga ctaagatacc ctcacagact catccgtggg aaccacggat     1380

gagtcgctct tcatgcattg gagacc                                          1406


<210>  8
<211>  2059
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CRC2 pseudogene

<400>  8
ggtctcacat tgcataatat tcatccgtgg gaaccacgga tgaataacca aaaagttaag       60

gcaattaagt ttttctaatg tgccctatac accccagcca agccaagata gcatctgacc      120

ttacccatct tacattattt tgcttaacct ctctccaaca aagaagttaa aactttatag      180

aatgcattat tcctgagcaa tagacttttt aataaaatac ctacgtctta cattctttgt      240

taggtcacct atgaccgtgt aaagtccaac atgcctgtta caagaaaaaa aaaaaatcca      300

gcacaaccta gagttataat ccaatcttta tttaaaaatc taatctgcca gtttaggcgc      360

caggctttct ggtcatgcac caggttctag ggccctcagg cacttccacg tcggcggtca      420

cggtgaagcc cagtctctcg tagaagggca ggtttctggg ggcgcttgtt tccaggaagg      480

cgggcacgcc agccctttca gcagcttcca cgccaggcag caccacagca gatcccagtc      540

ccttgccctg gtggtcaggt gacacgccca cggtggccag aaaccaggca ggctctttag      600

gtctgtgggg ggccagcagg ccttccatct gctgctgggc agccagtcta gagccgctca      660

gctcggccat tctaggtccg atctcggcga acacagcgcc ggcttccaca gactcagggg      720

ttgtccacac agccacagcg gcgccatcat cggccaccca cactttgccg atgtccaggc      780

ccactctggt cagaaacagt tcctgcagct cggtcactct ctcgatgtgt ctgtcggggt      840

ccacggtgtg tctggtggca gggtaatcgg cgaaggcagc ggccagtgtc cgcacagctc      900

taggcacatc gtccctggtg gccagccgca ctgtgggctt gtactcggta ctaccaccgc      960

tccctccacc actgcctccc ttgtacagct cgtccatgcc cagggtgatg ccagcggcgg     1020

tcacaaattc cagcagcacc atgtggtccc gcttctcgtt ggggtccttg ctcagcttgc     1080

tctgggtgct caggtagtgg ttgtcgggca gcagcacggg gccatctccg atgggggtgt     1140

tctgctggta gtggtcggcc agctgcacgg agccgtcctc gatgttgtgc cgggtcttga     1200

agttcacttt gatgccgttt ttctgcttgt cggcggtgat gtacaccttg tggctgttgt     1260

agttgtactc cagcttgtgg cccaggatat tgccgtcctc tttgaagtcg atgcccttca     1320

gctcgatccg gttcacgagg gtgtcgccct cgaacttcac ttcggccctg gtcttgtagt     1380

tgccgtcgtc cttaaagaag atggtccgtt cctgcacgta gccctcgggc atggcgctct     1440

tgaagaagtc gtgctgcttc atatggtcgg ggtatctggc gaagcactgc acgccgtagg     1500

taaaggtggt cacgagggta ggccaaggca cgggcagctt gccggtggtg cagatgaact     1560

tcagggtcag cttgccatat gtggcgtcgc cttcgccctc gccggacacg ctgaacttgt     1620

ggccgttcac gtcgccgtcg agttccacca gaatgggcac cacgccggtg aacagttcct     1680

cgcccttgct cacgcttcct ccgcttccac ctccgcttcc accgcgtttt ccaccaactc     1740

ggggagctga aactttcaca ggcttcacaa tcttttgctt aggtgctgcc tttgtaggtg     1800

cctgtaaaaa gataacacaa tattactcca caaaactttt gttaccctat tattacccat     1860

ctctcacgtc cagattgttt atgtagctta gatcactttc ttagtaattt aactgcattc     1920

ctaatgccaa tcttttaatg tgactaccct taaatattta aagcccatct ttacaatgaa     1980

agacaaagac taagataccc tcacagactg gatcatccgt gggaaccacg gatgatccgc     2040

tcttcatgca ttggagacc                                                  2059


<210>  9
<211>  378
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Single stranded oligonucleotide

<400>  9
ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt       60

ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg      120

atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct      180

gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa      240

tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt      300

ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc      360

acatttcccc gaaaagtg                                                    378


<210>  10
<211>  420
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Single stranded oligonucleotide

<400>  10
attgaagcat gcggcgtgca taattctctt actgtcatgc catgcgtaag ataccaccac       60

acccgcattc gccattcagg cggccgccac cgcggtggag ctccagctgc tgtttcctgt      120

gtagagttgg tagctcttga tccggtcata tttgttccct ttagatccgc ctccatctac      180

agggcgcgtc cccgcgctta atgcgcggcc taactacggc tacactagaa ggacttacct      240

tcggaaaaga aattgttatc cgctcacaaa agccagagta tttaagctcc ctcgtgcgct      300

ctcctgttcc gggttattgt ctcatcggcg accgagttgc tcttgcttat cagaccctgc      360

cgcttacaag tggtcgccag tctattaaca gcactcaata cgggataatt tttcaatatt      420


<210>  11
<211>  78
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Single stranded oligonucleotide

<400>  11
gaaccgtccc aagcgttgcg ccacatctgc tggaaggtgg acagtgagag gacacctacg       60

aatcgcaacg ggtatcct                                                     78


<210>  12
<211>  77
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Single stranded oligonucleotide

<400>  12
gaaccgtccc aagcgttgcg cctgggtaca tggtggtacc accagacagg acacctacga       60

atcgcaacgg gtatcct                                                      77


<210>  13
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Single stranded oligonucleotide

<400>  13
gaaccgtccc aagcgttgcg gagagcatag ccctcgtaga tgggcaagga cacctacgaa       60

tcgcaacggg tatcct                                                       76


<210>  14
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Single stranded oligonucleotide

<400>  14
gaaccgtccc aagcgttgcg gtcccagttg gtaacaatgc catgttcaat gaggacacct       60

acgaatcgca acgggtatcc t                                                 81


<210>  15
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Single stranded oligonucleotide

<400>  15
gaaccgtccc aagcgttgcg cggactcatc gtactcctgc ttgctgagga cacctacgaa       60

tcgcaacggg tatcct                                                       76


<210>  16
<211>  78
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Single stranded oligonucleotide

<400>  16
gaaccgtccc aagcgttgcg ttctctttga tgtcacgcac gatttcccag gacacctacg       60

aatcgcaacg ggtatcct                                                     78


<210>  17
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Single stranded oligonucleotide

<400>  17
gaaccgtccc aagcgttgcg ctcggtcagg atcttcatga ggtagtctgt aggacaccta       60

cgaatcgcaa cgggtatcct                                                   80


<210>  18
<211>  78
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Single stranded oligonucleotide

<400>  18
gaaccgtccc aagcgttgcg tttcacggtt ggccttaggg ttcaggggag gacacctacg       60

aatcgcaacg ggtatcct                                                     78


<210>  19
<211>  78
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Single stranded oligonucleotide

<400>  19
gaaccgtccc aagcgttgcg gtacttcagg gtcaggatac ctctcttgag gacacctacg       60

aatcgcaacg ggtatcct                                                     78


<210>  20
<211>  79
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Single stranded oligonucleotide

<400>  20
gaaccgtccc aagcgttgcg ctgctcgaag tctagagcaa catagcacaa ggacacctac       60

gaatcgcaac gggtatcct                                                    79


<210>  21
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Single stranded oligonucleotide

<400>  21
gaaccgtccc aagcgttgcg cctcgtcacc cacataggag tccttcagga cacctacgaa       60

tcgcaacggg tatcct                                                       76


<210>  22
<211>  461
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Pseudogene

<400>  22
ggtctcacat tgcataatta acatccgcgg aacgcggatg ttccggcgtc aatacgggat       60

aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg      120

cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca      180

cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga      240

aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc      300

ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata      360

tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg      420

catccgcgga acgcggatgc agctcttctg cattggagac c                          461


<210>  23
<211>  510
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Pseudogene

<400>  23
ggtctcacat tgcataaatt tcatccgtgg gaaccacgga tgaaattgaa gcatgcggcg       60

tgcataattc tcttactgtc atgccatgcg taagatacca ccacacccgc attcgccatt      120

caggcggccg ccaccgcggt ggagctccag ctgctgtttc ctgtgtagag ttggtagctc      180

ttgatccggt catatttgtt ccctttagat ccgcctccat ctacagggcg cgtccccgcg      240

cttaatgcgc ggcctaacta cggctacact agaaggactt accttcggaa aagaaattgt      300

tatccgctca caaaagccag agtatttaag ctccctcgtg cgctctcctg ttccgggtta      360

ttgtctcatc ggcgaccgag ttgctcttgc ttatcagacc ctgccgctta caagtggtcg      420

ccagtctatt aacagcactc aatacgggat aatttttcaa tattcatccg tgggaaccac      480

ggatgaatag ctcttcatgc attggagacc                                       510


<210>  24
<211>  1177
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Pseudogene

<400>  24
ggtctcacat tgcataatct tcatccgtgg gaaccacgga tgaagaaccg tcccaagcgt       60

tgcgccacat ctgctggaag gtggacagtg agaggacacc tacgaatcgc aacgggtatc      120

ctcatccgtg ggaaccacgg atgaggaacc gtcccaagcg ttgcgcctgg gtacatggtg      180

gtaccaccag acaggacacc tacgaatcgc aacgggtatc ctcatccgtg ggaaccacgg      240

atgaggaacc gtcccaagcg ttgcggagag catagccctc gtagatgggc aaggacacct      300

acgaatcgca acgggtatcc tcatccgtgg gaaccacgga tgaggaaccg tcccaagcgt      360

tgcggtccca gttggtaaca atgccatgtt caatgaggac acctacgaat cgcaacgggt      420

atcctcatcc gtgggaacca cggatgagga accgtcccaa gcgttgcgcg gactcatcgt      480

actcctgctt gctgaggaca cctacgaatc gcaacgggta tcctcatccg tgggaaccac      540

ggatgaggaa ccgtcccaag cgttgcgttc tctttgatgt cacgcacgat ttcccaggac      600

acctacgaat cgcaacgggt atcctcatcc gtgggaacca cggatgagga accgtcccaa      660

gcgttgcgct cggtcaggat cttcatgagg tagtctgtag gacacctacg aatcgcaacg      720

ggtatcctca tccgtgggaa ccacggatga ggaaccgtcc caagcgttgc gtttcacggt      780

tggccttagg gttcagggga ggacacctac gaatcgcaac gggtatcctc atccgtggga      840

accacggatg aggaaccgtc ccaagcgttg cggtacttca gggtcaggat acctctcttg      900

aggacaccta cgaatcgcaa cgggtatcct catccgtggg aaccacggat gaggaaccgt      960

cccaagcgtt gcgctgctcg aagtctagag caacatagca caaggacacc tacgaatcgc     1020

aacgggtatc ctcatccgtg ggaaccacgg atgaggaacc gtcccaagcg ttgcgcctcg     1080

tcacccacat aggagtcctt caggacacct acgaatcgca acgggtatcc tcatccgtgg     1140

gaaccacgga tgaggagctc ttcatgcatt ggagacc                              1177


<210>  25
<211>  7
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nt.BspQI cleavage domain

<400>  25
gctcttc                                                                  7


<210>  26
<211>  6
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nb.BsrDI cleavage domain

<400>  26
gcaatg                                                                   6


<210>  27
<211>  5455
<212>  DNA
<213>  Artificial sequence

<220>
<223>  pM2

<400>  27
aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct       60

tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca      120

aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg      180

attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg      240

atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc      300

taacaggagg aattaaccat ggggggttct catcatcatc atcatcatgg tatggctagc      360

ccacaatttg atatattatg taaaacacca cctaaggtgc ttgttcgtca gtttgtggaa      420

aggtttgaaa gaccttcagg tgagaaaata gcattatgtg ctgctgaact aacctattta      480

tgttggatga ttacacataa cggaacagca atcaagagag ccacattcat gagctataat      540

actatcataa gcaattcgct gagtttcgat attgtcaata aatcactcca gtttaaatac      600

aagacgcaaa aagcaacaat tctggaagcc tcattaaaga aattgattcc tgcttgggaa      660

tttacaatta ttccttacta tggacaaaaa catcaatctg atatcactga tattgtaagt      720

agtttgcaat tacagttcga atcatcggaa gaagcagata agggaaatag ccacagtaaa      780

aaaatgctta aagcacttct aagtgagggt gaaagcatct gggagatcac tgagaaaata      840

ctaaattcgt ttgagtatac ttcgagattt acaaaaacaa aaactttata ccaattcctc      900

ttcctagcta ctttcatcaa ttgtggaaga ttcagcgata ttaagaacgt tgatccgaaa      960

tcatttaaat tagtccaaaa taagtatctg ggagtaataa tccagtgttt agtgacagag     1020

acaaagacaa gcgttagtag gcacatatac ttctttagcg caaggggtag gatcgatcca     1080

cttgtatatt tggatgaatt tttgaggaat tctgaaccag tcctaaaacg agtaaatagg     1140

accggcaatt cttcaagcaa taaacaggaa taccaattat taaaagataa cttagtcaga     1200

tcgtacaata aagccttgaa gaaaaatgcg ccttattcaa tctttgctat aaaaaatggc     1260

ccaaaatctc acattggaag acatttgatg acctcatttc tttcaatgaa gggcctaacg     1320

gagttgacta atgttgtggg aaattggagc gataagcgtg cttctgccgt ggccaggaca     1380

acgtatactc atcagataac agcaatacct gatcactact tcgcactagt ttctcggtac     1440

tatgcatatg atccaatatc aaaggaaatg atagcattga aggatgagac taatccaatt     1500

gaggagtggc agcatataga acagctaaag ggtagtgctg aaggaagcat acgatacccc     1560

gcatggaatg ggataatatc acaggaggta ctagactacc tttcatccta cataaataga     1620

cgcatataac gcaaaaaacc ccgcttcggc ggggtttttt cgctaactat aacggtccta     1680

aggtagcgag aagttcctat actttttaga gaataggaac ttcctcgagg taccagaatt     1740

atcggatccc gggcccgtcg agaagttcct atacttttta gagaatagga acttcttaat     1800

taaggcgcgc ccaattgaag cttggctgtt ttggcggatg agagaagatt ttcagcctga     1860

tacagattaa atcagaacgc agaagcggtc tgataaaaca gaatttgcct ggcggcagta     1920

gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt agcgccgatg     1980

gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag     2040

gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg     2100

agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg     2160

cgggcaggac gcccgccata aactgccagg catcaaatta agcagaaggc catcctgacg     2220

gatggccttt ttgcgtttct acaaactctt ttgtttattt ttctaaatac attcaaatat     2280

gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag     2340

tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc     2400

tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc     2460

acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc     2520

cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc     2580

ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt     2640

ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt     2700

atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat     2760

cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct     2820

tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat     2880

gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc     2940

ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg     3000

ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc     3060

tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta     3120

cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc     3180

ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga     3240

tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat     3300

gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat     3360

caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa     3420

accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa     3480

ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt     3540

aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt     3600

accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata     3660

gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt     3720

ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac     3780

gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga     3840

gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg     3900

ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa     3960

aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat     4020

gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc     4080

tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga     4140

agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg     4200

gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta     4260

tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc     4320

tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc     4380

tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagca gatcaattcg     4440

cgcgcgaagg cgaagcggca tgcataatgt gcctgtcaaa tggacgaagc agggattctg     4500

caaaccctat gctactccgt caagccgtca attgtctgat tcgttaccaa ttatgacaac     4560

ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct cgggctggcc     4620

ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc aacattgcga     4680

ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg gctgatacgt     4740

tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag atgtgacaga     4800

cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt gctgtctgcc     4860

aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg tggatggagc     4920

gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt tatcgccagc     4980

agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa caggtcgctg     5040

aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa tattgacggc     5100

cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg gtgataccat     5160

tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa cagcaaaata     5220

tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg accgcgaatg     5280

gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa atcgagataa     5340

ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga gtatcccggc     5400

agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt cagag          5455


<210>  28
<211>  5041
<212>  DNA
<213>  Artificial sequence

<220>
<223>  pM3

<400>  28
aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct       60

tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca      120

aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg      180

attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg      240

atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc      300

taacaggagg aattaaccat ggggggttct catcatcatc atcatcatgg tatggctagc      360

gcgacccgcg aacagcagcc ggaaggccgc cgcctgaaag tggcgcgcat ttatctgcgc      420

gcgagcaccg atgaacagaa cctggaacgc caggaaagcc tggtggcggc gacccgcgcg      480

gcgggctatt atgtggcggg catttatcgc gaaaaagcga gcggcgcgcg cgcggatcgc      540

ccggaactgc tgcgcatgat tgcggatctg cagccgggcg aagtggtggt ggcggaaaaa      600

attgatcgca ttagccgcct gccgctggcg gaagcggaac gcctggtggc gagcattcgc      660

gcgaaaggcg cgaaactggc ggtgccgggc gtggtggatc tgagcgaact ggcggcggaa      720

gcgaacggcg tggcgaaaat tgtgctggaa agcgtgcagg atatgctgct gaaactggcg      780

ctgcagatgg cgcgcgatga ttatgaagat cgccgcgaac gccagcgcca gggcgtgcag      840

ctggcgaaag cggcgggccg ctataccggc cgcaaacgcg atgcgggcat gcatgatcgc      900

attattaccc tgcgcagcgg cggcagcagc attgcgaaaa ccgcgaaact ggtgggctgc      960

agcccgagcc aggtgaaacg cgtgtgggcg gcgtggaacg cgcagcagca gaaataacgc     1020

aaaaaacccc gcttcggcgg ggttttttcg ctaactataa cggtcctaag gtagcgaggt     1080

caaattgggt atacccattt gggcctagtc tagccggcat ggcgcattac agcaatacgc     1140

aatttaaatg cgcctagcgc attttcccga ccttaatgcg cctcgcgctg tagcctcacg     1200

cccacatatg ctcgaggtac cagaattatc ggatcccggg cccgtcgagg tcaaattggg     1260

tatacccatt tgggcctagt ctagccggca tggcgcatta cagcaatacg caatttaaat     1320

gcgcctagcg cattttcccg accttaatgc gcctcgcgct gtagcctcac gcccacatat     1380

gttaattaag gcgcgcccaa ttgaagcttg gctgttttgg cggatgagag aagattttca     1440

gcctgataca gattaaatca gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg     1500

gcagtagcgc ggtggtccca cctgacccca tgccgaactc agaagtgaaa cgccgtagcg     1560

ccgatggtag tgtggggtct ccccatgcga gagtagggaa ctgccaggca tcaaataaaa     1620

cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct     1680

ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga     1740

gggtggcggg caggacgccc gccataaact gccaggcatc aaattaagca gaaggccatc     1800

ctgacggatg gcctttttgc gtttctacaa actcttttgt ttatttttct aaatacattc     1860

aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag     1920

gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg     1980

ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt     2040

gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt     2100

tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt     2160

attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa     2220

tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag     2280

agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac     2340

aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac     2400

tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac     2460

cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac     2520

tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact     2580

tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg     2640

tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt     2700

tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat     2760

aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta     2820

gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa     2880

tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga     2940

aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac     3000

aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt     3060

tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc     3120

gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat     3180

cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag     3240

acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc     3300

cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag     3360

cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac     3420

aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg     3480

gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct     3540

atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc     3600

tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga     3660

gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga     3720

agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg     3780

catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact     3840

ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac     3900

gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc     3960

gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagcagatc     4020

aattcgcgcg cgaaggcgaa gcggcatgca taatgtgcct gtcaaatgga cgaagcaggg     4080

attctgcaaa ccctatgcta ctccgtcaag ccgtcaattg tctgattcgt taccaattat     4140

gacaacttga cggctacatc attcactttt tcttcacaac cggcacggaa ctcgctcggg     4200

ctggccccgg tgcatttttt aaatacccgc gagaaataga gttgatcgtc aaaaccaaca     4260

ttgcgaccga cggtggcgat aggcatccgg gtggtgctca aaagcagctt cgcctggctg     4320

atacgttggt cctcgcgcca gcttaagacg ctaatcccta actgctggcg gaaaagatgt     4380

gacagacgcg acggcgacaa gcaaacatgc tgtgcgacgc tggcgatatc aaaattgctg     4440

tctgccaggt gatcgctgat gtactgacaa gcctcgcgta cccgattatc catcggtgga     4500

tggagcgact cgttaatcgc ttccatgcgc cgcagtaaca attgctcaag cagatttatc     4560

gccagcagct ccgaatagcg cccttcccct tgcccggcgt taatgatttg cccaaacagg     4620

tcgctgaaat gcggctggtg cgcttcatcc gggcgaaaga accccgtatt ggcaaatatt     4680

gacggccagt taagccattc atgccagtag gcgcgcggac gaaagtaaac ccactggtga     4740

taccattcgc gagcctccgg atgacgaccg tagtgatgaa tctctcctgg cgggaacagc     4800

aaaatatcac ccggtcggca aacaaattct cgtccctgat ttttcaccac cccctgaccg     4860

cgaatggtga gattgagaat ataacctttc attcccagcg gtcggtcgat aaaaaaatcg     4920

agataaccgt tggcctcaat cggcgttaaa cccgccacca gatgggcatt aaacgagtat     4980

cccggcagca ggggatcatt ttgcgcttca gccatacttt tcatactccc gccattcaga     5040

g                                                                     5041


<210>  29
<211>  133
<212>  DNA
<213>  Artificial sequence

<220>
<223>  msr_l (ParA resolvase attachment site)

<400>  29
ggtcaaattg ggtataccca tttgggccta gtctagccgg catggcgcat tacagcaata       60

cgcaatttaa atgcgcctag cgcattttcc cgaccttaat gcgcctcgcg ctgtagcctc      120

acgcccacat atg                                                         133


<210>  30
<211>  133
<212>  DNA
<213>  Artificial sequence

<220>
<223>  msr_r (ParA resolvase attachment site)

<400>  30
ggtcaaattg ggtataccca tttgggccta gtctagccgg catggcgcat tacagcaata       60

cgcaatttaa atgcgcctag cgcattttcc cgaccttaat gcgcctcgcg ctgtagcctc      120

acgcccacat atg                                                         133


<210>  31
<211>  34
<212>  DNA
<213>  Artificial sequence

<220>
<223>  FLPr (FLP recombinase attachment site)

<400>  31
gaagttccta tactttttag agaataggaa cttc                                   34


<210>  32
<211>  34
<212>  DNA
<213>  Artificial sequence

<220>
<223>  FLPl (FLP recombinase attachment site)

<400>  32
gaagttccta tactttttag agaataggaa cttc                                   34


