﻿                         序列表

<110>  浙江诺迦生物科技有限公司
       杭州迈尔德生物科技有限公司

<120>  一种基于S蛋白配体与ACE2受体竞争法层析的冠状病毒快速检测的试剂盒

<160>  3

<170>  SIPOSequenceListing 1.0

<210>  1
<211>  2987
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  1
gaattcatga gcagcagcag ctggctgctg ctgagcctgg tggccgtgac cgccgctcag        60
tccaccatcg aggagcaggc caagaccttc ctggacaagt tcaatcacga ggccgaggac       120
ctgttttacc agagcagcct ggcctcctgg aactacaaca ccaatatcac cgaggagaac       180
gtgcagaaca tgaataacgc cggcgataag tggagcgcct tcctgaagga gcagagcacc       240
ctggcccaga tgtaccccct gcaggagatc cagaacctga ccgtgaagct gcagctgcag       300
gccctgcagc agaatggctc cagcgtgctg agcgaggata agtccaagag actgaatacc       360
atcctgaata ccatgtccac catctactcc accggcaagg tgtgcaatcc tgacaatcct       420
caggagtgtc tgctgctgga gcccggcctg aacgagatca tggccaacag cctggattac       480
aatgagaggc tgtgggcctg ggagtcctgg aggagcgagg tgggcaagca gctgaggcct       540
ctgtacgagg agtacgtggt gctgaagaac gagatggcca gggccaacca ctacgaggat       600
tacggcgatt actggagggg cgattacgag gtgaatggcg tggatggcta cgattactcc       660
agaggccagc tgatcgagga tgtggagcac accttcgagg agatcaagcc tctgtacgaa       720
cacctgcacg cctacgtgag agccaagctg atgaacgcct acccctccta catctccccc       780
atcggctgcc tgcctgccca cctgctgggc gatatgtggg gcagattctg gaccaatctg       840
tactccctga ccgtgccttt cggccagaag cccaatatcg atgtgaccga cgccatggtg       900
gatcaggcct gggacgccca gagaatcttt aaggaggccg agaagttctt tgtgtccgtg       960
ggcctgccca atatgaccca gggcttctgg gagaatagca tgctgaccga ccctggcaat      1020
gtgcagaagg ccgtgtgcca ccccaccgcc tgggacctgg gaaagggcga ctttagaatc      1080
ctgatgtgca ccaaggtgac catggatgac ttcctgaccg cccaccacga gatgggccac      1140
atccagtacg acatggccta cgccgcccag cccttcctgc tgaggaatgg cgccaatgag      1200
ggcttccacg aggccgtggg cgagatcatg tccctgtccg ccgccacccc caagcacctg      1260
aagagcatcg gcctgctgag ccctgatttt caggaggaca atgagacaga gatcaacttt      1320
ctgctgaagc aggccctgac catcgtgggc accctgccct tcacctacat gctggagaag      1380
tggaggtgga tggtgtttaa gggcgagatc cctaaggacc agtggatgaa gaagtggtgg      1440
gagatgaaga gagagatcgt gggcgtggtg gagcccgtgc cccacgatga gacatactgt      1500
gaccctgcct ccctgtttca cgtgtccaat gactacagct tcatcaggta ctacaccagg      1560
accctgtacc agtttcagtt ccaggaggcc ctgtgtcagg ccgccaagca cgagggcccc      1620
ctgcataagt gtgatatcag caactccacc gaggccggcc agaagctgtt taatatgctg      1680
aggctgggca agagcgagcc ctggaccctg gccctggaga acgtggtggg cgccaagaat      1740
atgaacgtga ggcccctgct gaactacttt gagcccctgt tcacctggct gaaggaccag      1800
aacaagaact cctttgtggg ctggagcacc gactggtccc cctacgccga ccagagcatc      1860
aaggtgagga tctccctgaa gtccgccctg ggcgatagag cctacgagtg gaacgataac      1920
gagatgtacc tgttcaggag ctccgtggcc tacgccatga ggcagtactt cctgaaggtg      1980
aagaatcaga tgatcctgtt cggcgaggag gatgtgaggg tggccaacct gaagcccaga      2040
atctccttca actttttcgt gaccgccccc aagaacgtgt ccgacatcat ccctaggacc      2100
gaggtggaga aggccatcag aatgtccagg tccagaatca acgacgcctt cagactgaat      2160
gataacagcc tggagttcct gggcatccag cccaccctgg gcccccctaa ccagccacct      2220
gtgggcagcg gcggcagcgg cggaagcgct ggaggaggac tgaacgatat cttcgaggcc      2280
cagaagatcg agtggctcga ggattacaag gacgatgacg ataagatatg tacagtccca      2340
gaagtatcat ctgtcttcat cttcccccca aagcccaagg atgtgctcat gattactctg      2400
actcctaagg tcacgtgtgt tgtggtagac atcagcaagg atgatcccga ggtccagttc      2460
agctggtttg tagatgatgt ggaggtgcac acagctcaga cgcaaccccg ggaggagcag      2520
ttcaacagca ctttccgctc agtcagtgaa cttcccatca tgcaccagga ctggctcaat      2580
ggcaaggagt tcaaatgcag ggtcaacagt gcagctttcc ctgcccccat cgagaaaacc      2640
atctccaaaa ccaaaggcag accgaaggct ccacaggtgt acaccatccc acctcccaag      2700
gagcagatgg ccaaggataa agtcagtctg acctgcatga taacagactt cttccctgaa      2760
gacattactg tggagtggca gtggaatggg cagccagcgg agaactacaa gaacactcag      2820
cccatcatgg acacagatgg ctcttacttc gtctacagca agctcaatgt gcagaagagc      2880
aactgggagg caggaaatac tttcacctgc tctgtgttac atgagggcct gcacaaccac      2940
catactgaga agagcctctc ccactctcct ggtaaatgag cggccgc                    2987

<210>  2
<211>  990
<212>  PRT
<213>  pCDH-ACE2mFc.copGFP质粒(pCDH-ACE2mFc.copGFP plasmid)

<400>  2
Met Ser Ser Ser Ser Trp Leu Leu Leu Ser Leu Val Ala Val Thr Ala 
1               5                   10                  15      
Ala Gln Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe 
            20                  25                  30          
Asn His Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp 
        35                  40                  45              
Asn Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn 
    50                  55                  60                  
Ala Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala 
65                  70                  75                  80  
Gln Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln 
                85                  90                  95      
Leu Gln Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp Lys 
            100                 105                 110         
Ser Lys Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser 
        115                 120                 125             
Thr Gly Lys Val Cys Asn Pro Asp Asn Pro Gln Glu Cys Leu Leu Leu 
    130                 135                 140                 
Glu Pro Gly Leu Asn Glu Ile Met Ala Asn Ser Leu Asp Tyr Asn Glu 
145                 150                 155                 160 
Arg Leu Trp Ala Trp Glu Ser Trp Arg Ser Glu Val Gly Lys Gln Leu 
                165                 170                 175     
Arg Pro Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg 
            180                 185                 190         
Ala Asn His Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu 
        195                 200                 205             
Val Asn Gly Val Asp Gly Tyr Asp Tyr Ser Arg Gly Gln Leu Ile Glu 
    210                 215                 220                 
Asp Val Glu His Thr Phe Glu Glu Ile Lys Pro Leu Tyr Glu His Leu 
225                 230                 235                 240 
His Ala Tyr Val Arg Ala Lys Leu Met Asn Ala Tyr Pro Ser Tyr Ile 
                245                 250                 255     
Ser Pro Ile Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly 
            260                 265                 270         
Arg Phe Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Gly Gln Lys 
        275                 280                 285             
Pro Asn Ile Asp Val Thr Asp Ala Met Val Asp Gln Ala Trp Asp Ala 
    290                 295                 300                 
Gln Arg Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu 
305                 310                 315                 320 
Pro Asn Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Asp Pro 
                325                 330                 335     
Gly Asn Val Gln Lys Ala Val Cys His Pro Thr Ala Trp Asp Leu Gly 
            340                 345                 350         
Lys Gly Asp Phe Arg Ile Leu Met Cys Thr Lys Val Thr Met Asp Asp 
        355                 360                 365             
Phe Leu Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala 
    370                 375                 380                 
Tyr Ala Ala Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe 
385                 390                 395                 400 
His Glu Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys 
                405                 410                 415     
His Leu Lys Ser Ile Gly Leu Leu Ser Pro Asp Phe Gln Glu Asp Asn 
            420                 425                 430         
Glu Thr Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly 
        435                 440                 445             
Thr Leu Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe 
    450                 455                 460                 
Lys Gly Glu Ile Pro Lys Asp Gln Trp Met Lys Lys Trp Trp Glu Met 
465                 470                 475                 480 
Lys Arg Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr 
                485                 490                 495     
Tyr Cys Asp Pro Ala Ser Leu Phe His Val Ser Asn Asp Tyr Ser Phe 
            500                 505                 510         
Ile Arg Tyr Tyr Thr Arg Thr Leu Tyr Gln Phe Gln Phe Gln Glu Ala 
        515                 520                 525             
Leu Cys Gln Ala Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile 
    530                 535                 540                 
Ser Asn Ser Thr Glu Ala Gly Gln Lys Leu Phe Asn Met Leu Arg Leu 
545                 550                 555                 560 
Gly Lys Ser Glu Pro Trp Thr Leu Ala Leu Glu Asn Val Val Gly Ala 
                565                 570                 575     
Lys Asn Met Asn Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe 
            580                 585                 590         
Thr Trp Leu Lys Asp Gln Asn Lys Asn Ser Phe Val Gly Trp Ser Thr 
        595                 600                 605             
Asp Trp Ser Pro Tyr Ala Asp Gln Ser Ile Lys Val Arg Ile Ser Leu 
    610                 615                 620                 
Lys Ser Ala Leu Gly Asp Arg Ala Tyr Glu Trp Asn Asp Asn Glu Met 
625                 630                 635                 640 
Tyr Leu Phe Arg Ser Ser Val Ala Tyr Ala Met Arg Gln Tyr Phe Leu 
                645                 650                 655     
Lys Val Lys Asn Gln Met Ile Leu Phe Gly Glu Glu Asp Val Arg Val 
            660                 665                 670         
Ala Asn Leu Lys Pro Arg Ile Ser Phe Asn Phe Phe Val Thr Ala Pro 
        675                 680                 685             
Lys Asn Val Ser Asp Ile Ile Pro Arg Thr Glu Val Glu Lys Ala Ile 
    690                 695                 700                 
Arg Met Ser Arg Ser Arg Ile Asn Asp Ala Phe Arg Leu Asn Asp Asn 
705                 710                 715                 720 
Ser Leu Glu Phe Leu Gly Ile Gln Pro Thr Leu Gly Pro Pro Asn Gln 
                725                 730                 735     
Pro Pro Val Gly Ser Gly Gly Ser Gly Gly Ser Ala Gly Gly Gly Leu 
            740                 745                 750         
Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp Leu Glu Asp Tyr Lys 
        755                 760                 765             
Asp Asp Asp Asp Lys Ile Cys Thr Val Pro Glu Val Ser Ser Val Phe 
    770                 775                 780                 
Ile Phe Pro Pro Lys Pro Lys Asp Val Leu Met Ile Thr Leu Thr Pro 
785                 790                 795                 800 
Lys Val Thr Cys Val Val Val Asp Ile Ser Lys Asp Asp Pro Glu Val 
                805                 810                 815     
Gln Phe Ser Trp Phe Val Asp Asp Val Glu Val His Thr Ala Gln Thr 
            820                 825                 830         
Gln Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Ser Val Ser Glu 
        835                 840                 845             
Leu Pro Ile Met His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys Cys 
    850                 855                 860                 
Arg Val Asn Ser Ala Ala Phe Pro Ala Pro Ile Glu Lys Thr Ile Ser 
865                 870                 875                 880 
Lys Thr Lys Gly Arg Pro Lys Ala Pro Gln Val Tyr Thr Ile Pro Pro 
                885                 890                 895     
Pro Lys Glu Gln Met Ala Lys Asp Lys Val Ser Leu Thr Cys Met Ile 
            900                 905                 910         
Thr Asp Phe Phe Pro Glu Asp Ile Thr Val Glu Trp Gln Trp Asn Gly 
        915                 920                 925             
Gln Pro Ala Glu Asn Tyr Lys Asn Thr Gln Pro Ile Met Asp Thr Asp 
    930                 935                 940                 
Gly Ser Tyr Phe Val Tyr Ser Lys Leu Asn Val Gln Lys Ser Asn Trp 
945                 950                 955                 960 
Glu Ala Gly Asn Thr Phe Thr Cys Ser Val Leu His Glu Gly Leu His 
                965                 970                 975     
Asn His His Thr Glu Lys Ser Leu Ser His Ser Pro Gly Lys 
            980                 985                 990 

<210>  3
<211>  756
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  3
Gln Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe Asn 
1               5                   10                  15      
His Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp Asn 
            20                  25                  30          
Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn Ala 
        35                  40                  45              
Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala Gln 
    50                  55                  60                  
Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln Leu 
65                  70                  75                  80  
Gln Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp Lys Ser 
                85                  90                  95      
Lys Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser Thr 
            100                 105                 110         
Gly Lys Val Cys Asn Pro Asp Asn Pro Gln Glu Cys Leu Leu Leu Glu 
        115                 120                 125             
Pro Gly Leu Asn Glu Ile Met Ala Asn Ser Leu Asp Tyr Asn Glu Arg 
    130                 135                 140                 
Leu Trp Ala Trp Glu Ser Trp Arg Ser Glu Val Gly Lys Gln Leu Arg 
145                 150                 155                 160 
Pro Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg Ala 
                165                 170                 175     
Asn His Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Val 
            180                 185                 190         
Asn Gly Val Asp Gly Tyr Asp Tyr Ser Arg Gly Gln Leu Ile Glu Asp 
        195                 200                 205             
Val Glu His Thr Phe Glu Glu Ile Lys Pro Leu Tyr Glu His Leu His 
    210                 215                 220                 
Ala Tyr Val Arg Ala Lys Leu Met Asn Ala Tyr Pro Ser Tyr Ile Ser 
225                 230                 235                 240 
Pro Ile Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg 
                245                 250                 255     
Phe Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Gly Gln Lys Pro 
            260                 265                 270         
Asn Ile Asp Val Thr Asp Ala Met Val Asp Gln Ala Trp Asp Ala Gln 
        275                 280                 285             
Arg Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu Pro 
    290                 295                 300                 
Asn Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Asp Pro Gly 
305                 310                 315                 320 
Asn Val Gln Lys Ala Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys 
                325                 330                 335     
Gly Asp Phe Arg Ile Leu Met Cys Thr Lys Val Thr Met Asp Asp Phe 
            340                 345                 350         
Leu Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala Tyr 
        355                 360                 365             
Ala Ala Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His 
    370                 375                 380                 
Glu Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys His 
385                 390                 395                 400 
Leu Lys Ser Ile Gly Leu Leu Ser Pro Asp Phe Gln Glu Asp Asn Glu 
                405                 410                 415     
Thr Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr 
            420                 425                 430         
Leu Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys 
        435                 440                 445             
Gly Glu Ile Pro Lys Asp Gln Trp Met Lys Lys Trp Trp Glu Met Lys 
    450                 455                 460                 
Arg Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr Tyr 
465                 470                 475                 480 
Cys Asp Pro Ala Ser Leu Phe His Val Ser Asn Asp Tyr Ser Phe Ile 
                485                 490                 495     
Arg Tyr Tyr Thr Arg Thr Leu Tyr Gln Phe Gln Phe Gln Glu Ala Leu 
            500                 505                 510         
Cys Gln Ala Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile Ser 
        515                 520                 525             
Asn Ser Thr Glu Ala Gly Gln Lys Leu Phe Asn Met Leu Arg Leu Gly 
    530                 535                 540                 
Lys Ser Glu Pro Trp Thr Leu Ala Leu Glu Asn Val Val Gly Ala Lys 
545                 550                 555                 560 
Asn Met Asn Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe Thr 
                565                 570                 575     
Trp Leu Lys Asp Gln Asn Lys Asn Ser Phe Val Gly Trp Ser Thr Asp 
            580                 585                 590         
Trp Ser Pro Tyr Ala Asp Gln Ser Ile Lys Val Arg Ile Ser Leu Lys 
        595                 600                 605             
Ser Ala Leu Gly Asp Arg Ala Tyr Glu Trp Asn Asp Asn Glu Met Tyr 
    610                 615                 620                 
Leu Phe Arg Ser Ser Val Ala Tyr Ala Met Arg Gln Tyr Phe Leu Lys 
625                 630                 635                 640 
Val Lys Asn Gln Met Ile Leu Phe Gly Glu Glu Asp Val Arg Val Ala 
                645                 650                 655     
Asn Leu Lys Pro Arg Ile Ser Phe Asn Phe Phe Val Thr Ala Pro Lys 
            660                 665                 670         
Asn Val Ser Asp Ile Ile Pro Arg Thr Glu Val Glu Lys Ala Ile Arg 
        675                 680                 685             
Met Ser Arg Ser Arg Ile Asn Asp Ala Phe Arg Leu Asn Asp Asn Ser 
    690                 695                 700                 
Leu Glu Phe Leu Gly Ile Gln Pro Thr Leu Gly Pro Pro Asn Gln Pro 
705                 710                 715                 720 
Pro Val Gly Ser Gly Gly Ser Gly Gly Ser Ala Gly Gly Gly Leu Asn 
                725                 730                 735     
Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp Leu Glu Asp Tyr Lys Asp 
            740                 745                 750         
Asp Asp Asp Lys 
        755     

