                         SEQUENCE LISTING

<110>  Mayo Foundation for Medical Education and Research
 
<120>  METHODS AND MATERIALS FOR TREATING CANCER

<130>  07039-1957WO1

<140>  PCT/US2021/018484
<141>  2021-02-18

<150>  62/978,208
<151>  2020-02-18

<160>  50    

<170>  PatentIn version 3.5

<210>  1
<211>  19
<212>  RNA
<213>  Artificial

<220>
<223>  siRNA targeting FOXA1

<400>  1
gagagaaaaa aucaacagc                                                    19


<210>  2
<211>  19
<212>  RNA
<213>  Artificial

<220>
<223>  siRNA targeting FOXA1

<400>  2
gcacugcaau acucgccuu                                                    19


<210>  3
<211>  19
<212>  RNA
<213>  Artificial

<220>
<223>  non-targeting control siRNA

<400>  3
uagcgacuaa acacaucaa                                                    19


<210>  4
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide primer

<400>  4
ccgggaaact gtggcgtgat gg                                                22


<210>  5
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide primer

<400>  5
aggtggagga gtgggtgtcg ctgtt                                             25


<210>  6
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide primer

<400>  6
agatggaagg gcatgaaacc a                                                 21


<210>  7
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide primer

<400>  7
atgttgccgc tcgtagtcat                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide primer

<400>  8
gttcatcctg ggctccattg                                                   20


<210>  9
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide primer

<400>  9
cccctggcat ggttctctt                                                    19


<210>  10
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide primer

<400>  10
gagaacggga aggagacgc                                                    19


<210>  11
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide primer

<400>  11
catctcaggg tgaggggct                                                    19


<210>  12
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide primer

<400>  12
gagaggactt gtctgcacat c                                                 21


<210>  13
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide primer

<400>  13
gcatccacat aaccatagat aaagg                                             25


<210>  14
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide primer

<400>  14
agaaggtggg aaaatggtac c                                                 21


<210>  15
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide primer

<400>  15
gttggcaaag cttcgaactg                                                   20


<210>  16
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide primer

<400>  16
cagcttgact caaaattcct gga                                               23


<210>  17
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide primer

<400>  17
tgaagattac gcttgctttt cct                                               23


<210>  18
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide primer

<400>  18
cacgcagaaa accacacttc                                                   20


<210>  19
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide primer

<400>  19
tgttcctcct tgtgcatctt c                                                 21


<210>  20
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide primer

<400>  20
tggtacatgt ggctttgctc                                                   20


<210>  21
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide primer

<400>  21
ccaccgagat gtcagaaaga g                                                 21


<210>  22
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide primer

<400>  22
tgcactgagg cagatgctgc g                                                 21


<210>  23
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide primer

<400>  23
tcattgcggc acaccagtac ag                                                22


<210>  24
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide primer

<400>  24
actgagtaca acaaagccat ttga                                              24


<210>  25
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide primer

<400>  25
ttgtgacatt gtcctgtccc cac                                               23


<210>  26
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide primer

<400>  26
cctacattag tgccttacgg g                                                 21


<210>  27
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide primer

<400>  27
tccatttcgc agatagtaca gc                                                22


<210>  28
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide primer

<400>  28
gagccgtacc tgtcaccct                                                    19


<210>  29
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide primer

<400>  29
gggccgtata ggaacgtgc                                                    19


<210>  30
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide primer

<400>  30
gcgcagatca cccagaagat                                                   20


<210>  31
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide primer

<400>  31
tcctcaccag gatgctcaga                                                   20


<210>  32
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide primer

<400>  32
agacaggacc atcggaatct                                                   20


<210>  33
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide primer

<400>  33
gtaacccttc ttcaggtgga ac                                                22


<210>  34
<211>  88
<212>  DNA
<213>  Artificial

<220>
<223>  nucleic acid encoding a FOXA1 luciferase reporter

<400>  34
tcgatgttta cttacagtat gtttacttta tccgtgttta catagtctat atttacttac       60

catatgtttg cttagtcatg tttactca                                          88


<210>  35
<211>  60
<212>  DNA
<213>  Artificial

<220>
<223>  forkhead response element

<400>  35
acatattgta tcgattgtcc ttgacagtaa acaaatctgt tgtaagagac attatcttta       60


<210>  36
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  interferon-stimulated response element probe

<400>  36
ctcccctgag tttcacttct tctcccaact tg                                     32


<210>  37
<211>  102
<212>  PRT
<213>  Artificial

<220>
<223>  fragment of a FOXA1 polypeptide

<400>  37

His Ala Lys Pro Pro Tyr Ser Tyr Ile Ser Leu Ile Thr Met Ala Ile 
1               5                   10                  15      


Gln Gln Ala Pro Ser Lys Met Leu Thr Leu Ser Glu Ile Tyr Gln Trp 
            20                  25                  30          


Ile Met Asp Leu Phe Pro Tyr Tyr Arg Gln Asn Gln Gln Arg Trp Gln 
        35                  40                  45              


Asn Ser Ile Arg His Ser Leu Ser Phe Asn Asp Cys Phe Val Lys Val 
    50                  55                  60                  


Ala Arg Ser Pro Asp Lys Pro Gly Lys Gly Ser Tyr Trp Thr Leu His 
65                  70                  75                  80  


Pro Asp Ser Gly Asn Met Phe Glu Asn Gly Cys Tyr Leu Arg Arg Gln 
                85                  90                  95      


Lys Arg Phe Lys Cys Glu 
            100         


<210>  38
<211>  17
<212>  DNA
<213>  Artificial

<220>
<223>  antisense oligonucleotide targeting FOXA1


<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  locked nucleic acids

<220>
<221>  misc_feature
<222>  (1)..(16)
<223>  phosphorothioate (PS) backbone

<220>
<221>  misc_feature
<222>  (15)..(17)
<223>  locked nucleic acids

<400>  38
atcagcatgg ccatcca                                                      17


<210>  39
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  antisense oligonucleotide targeting FOXA1


<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  locked nucleic acids

<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  PS backbone

<220>
<221>  misc_feature
<222>  (17)..(19)
<223>  locked nucleic acids

<400>  39
accacccgtt ctccatcaa                                                    19


<210>  40
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  antisense oligonucleotide targeting FOXA1


<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  locked nucleic acids

<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  PS backbone

<220>
<221>  misc_feature
<222>  (18)..(20)
<223>  locked nucleic acids

<400>  40
actcgcctta cggctctacg                                                   20


<210>  41
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  antisense oligonucleotide targeting FOXA1


<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  locked nucleic acids

<220>
<221>  misc_feature
<222>  (1)..(22)
<223>  PS backbone

<220>
<221>  misc_feature
<222>  (21)..(23)
<223>  locked nucleic acids

<400>  41
ccattttaat cattgccatc gtg                                               23


<210>  42
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  antisense oligonucleotide targeting FOXA1


<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  locked nucleic acids

<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  PS backbone

<220>
<221>  misc_feature
<222>  (18)..(20)
<223>  locked nucleic acids

<400>  42
ggtagcgcca taaggagagt                                                   20


<210>  43
<211>  16
<212>  DNA
<213>  Artificial

<220>
<223>  antisense oligonucleotide targeting FOXA1


<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  locked nucleic acids

<220>
<221>  misc_feature
<222>  (1)..(15)
<223>  PS backbone

<220>
<221>  misc_feature
<222>  (14)..(16)
<223>  locked nucleic acids

<400>  43
tggatggcca tcgtga                                                       16


<210>  44
<211>  17
<212>  DNA
<213>  Artificial

<220>
<223>  control antisense oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  locked nucleic acids

<220>
<221>  misc_feature
<222>  (1)..(16)
<223>  PS backbone

<220>
<221>  misc_feature
<222>  (15)..(17)
<223>  locked nucleic acids

<400>  44
gacgcgcctg agaggtt                                                      17


<210>  45
<211>  17
<212>  DNA
<213>  Artificial

<220>
<223>  antisense oligonucleotide targeting FOXA1

<400>  45
atcagcatgg ccatcca                                                      17


<210>  46
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  antisense oligonucleotide targeting FOXA1

<400>  46
accacccgtt ctccatcaa                                                    19


<210>  47
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  antisense oligonucleotide targeting FOXA1

<400>  47
actcgcctta cggctctacg                                                   20


<210>  48
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  antisense oligonucleotide targeting FOXA1

<400>  48
ccattttaat cattgccatc gtg                                               23


<210>  49
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  antisense oligonucleotide targeting FOXA1

<400>  49
ggtagcgcca taaggagagt                                                   20


<210>  50
<211>  16
<212>  DNA
<213>  Artificial

<220>
<223>  antisense oligonucleotide targeting FOXA1

<400>  50
tggatggcca tcgtga                                                       16


