                         SEQUENCE LISTING

<110>  New York Genome Center, Inc.
       New York University
 
<120>  METHODS AND COMPOSITIONS INVOLVING CRISPR CLASS 2, TYPE VI GUIDES

<130>  NYG-LIPP120PCT

<150>  US62/940575
<151>  2019-11-26

<150>  US62/952922
<151>  2019-12-23

<150>  US63/060757
<151>  2020-08-04

<160>  48    

<170>  PatentIn version 3.5

<210>  1
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop

<400>  1
aaccccuacc aacuggucgg gguuugaaac                                        30


<210>  2
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop sequence

<400>  2
uaccccuacc aacuggucgg gguuugaaac                                        30


<210>  3
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop sequence

<400>  3
uuccccuacc aacuggucgg gguuugaaac                                        30


<210>  4
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop sequence

<400>  4
uuucccuacc aacuggucgg gguuugaaac                                        30


<210>  5
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop sequence

<400>  5
uuacccuacc aacuggucgg gguuugaaac                                        30


<210>  6
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop sequence

<400>  6
aaccccgacc aacuggucgg gguuugaaac                                        30


<210>  7
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop sequence

<400>  7
uaccccgacc aacuggucgg gguuugaaac                                        30


<210>  8
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop sequence

<400>  8
uuccccgacc aacuggucgg gguuugaaac                                        30


<210>  9
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop sequence

<400>  9
uuucccgacc aacuggucgg gguuugaaac                                        30


<210>  10
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop sequence

<400>  10
aaccccuacc aacugguagg gguuugaaac                                        30


<210>  11
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop sequence

<400>  11
uaccccuacc aacugguagg gguuugaaac                                        30


<210>  12
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop sequence

<400>  12
uuccccuacc aacugguagg gguuugaaac                                        30


<210>  13
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop sequence

<400>  13
uuucccuacc aacugguagg gguuugaaac                                        30


<210>  14
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  B is one of C or G or U

<400>  14
baccccuacc aacuggucgg gguuugaaac                                        30


<210>  15
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  N is A or C or G or U

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  B is C or G or U

<400>  15
nbccccuacc aacuggucgg gguuugaaac                                        30


<210>  16
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  N is C or A or G or U

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  N is C or A or G or U

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  D is A or G or U

<400>  16
nndcccuacc aacuggucgg gguuugaaac                                        30


<210>  17
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop sequence


<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  V is A or C or G

<400>  17
aaccccuacc aacuggucgg gguvugaaac                                        30


<210>  18
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop sequence


<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  V is A or C or G

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  N is A or C or G or U

<400>  18
uuacccuacc aacuggucgg ggvnugaaac                                        30


<210>  19
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop sequence


<220>
<221>  misc_feature
<222>  (22)..(22)
<223>  V is A or C or U

<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  N is A or C or G or U

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  N is A or C or G or U

<400>  19
aaccccuacc aacuggucgg ghnnugaaac                                        30


<210>  20
<211>  29
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop sequence

<400>  20
accccuacca acuggucggg guuugaaac                                         29


<210>  21
<211>  28
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop sequence

<400>  21
ccccuaccaa cuggucgggg uuugaaac                                          28


<210>  22
<211>  27
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop sequence

<400>  22
cccuaccaac uggucggggu uugaaac                                           27


<210>  23
<211>  31
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  N is at least one additional A and/or C and/or G and/or U

<400>  23
naaccccuac caacuggucg ggguuugaaa c                                      31


<210>  24
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  V is A or C or G

<400>  24
aaccccvacc aacuggucgg gguuugaaac                                        30


<210>  25
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  B is one of C or G or U

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  V is A or C or G

<400>  25
baccccvacc aacuggucgg gguuugaaac                                        30


<210>  26
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  N is A or C or G or U

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  B is C or G or U

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  V is A or C or G

<400>  26
nbccccvacc aacuggucgg gguuugaaac                                        30


<210>  27
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  N is A or C or G or U

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  N is A or C or G or U

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  D is A or G or U

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  V is A or C or G

<400>  27
nndcccvacc aacuggucgg gguuugaaac                                        30


<210>  28
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  V is A or C or G

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  V is A or C or G

<400>  28
aaccccvacc aacuggucgg gguvugaaac                                        30


<210>  29
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  V is A or C or G

<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  V is A or C or G

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  N is A or C or G or U

<400>  29
uuacccvacc aacuggucgg ggvnugaaac                                        30


<210>  30
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  V is A or C or G

<220>
<221>  misc_feature
<222>  (22)..(22)
<223>  H is A or C or U

<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  N is A or C or G or U

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  N is A or C or G or U

<400>  30
aaccccvacc aacuggucgg ghnnugaaac                                        30


<210>  31
<211>  29
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop


<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  V is A or C or G

<400>  31
accccvacca acuggucggg guuugaaac                                         29


<210>  32
<211>  28
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop


<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  V is A or C or G

<400>  32
ccccvaccaa cuggucgggg uuugaaac                                          28


<210>  33
<211>  27
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop


<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  V is A or C or G

<400>  33
cccvaccaac uggucggggu uugaaac                                           27


<210>  34
<211>  31
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  N is at least one additional A and/or C and/or G and/or U

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  V is A or G or C

<400>  34
naaccccvac caacuggucg ggguuugaaa c                                      31


<210>  35
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop


<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  D is A or G or U

<400>  35
aaccccuacc aacuggudgg gguuugaaac                                        30


<210>  36
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  B is one of C or G or U

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  D is A or G or U

<400>  36
baccccuacc aacuggudgg gguuugaaac                                        30


<210>  37
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  N is A or C or G or U

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  B is C or G or U

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  D is A or G or U

<400>  37
nbccccuacc aacuggudgg gguuugaaac                                        30


<210>  38
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  N is A or C or G or U

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  N is A or C or G or U

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  D is A or G or U

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  D is A or G or U

<400>  38
nndcccuacc aacuggudgg gguuugaaac                                        30


<210>  39
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop


<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  D is A or G or U

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  V is A or C or G

<400>  39
aaccccuacc aacuggudgg gguvugaaac                                        30


<210>  40
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop


<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  D is A or G or U

<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  D is A or C or G

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  N is A or C or G or U

<400>  40
uuacccuacc aacuggudgg ggvnugaaac                                        30


<210>  41
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop


<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  D is A or G or U

<220>
<221>  misc_feature
<222>  (22)..(22)
<223>  H is A or C or U

<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  N is A or C or G or U

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  N is A or C or G or U

<400>  41
aaccccuacc aacuggudgg ghnnugaaac                                        30


<210>  42
<211>  29
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop


<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  D is A or G or U

<400>  42
accccuacca acuggudggg guuugaaac                                         29


<210>  43
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop


<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  D is A or G or U

<400>  43
ccccuaccaa cuggudgggg uuugaaac                                          28


<210>  44
<211>  27
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop


<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  D is A or G or U

<400>  44
cccuaccaac uggudggggu uugaaac                                           27


<210>  45
<211>  31
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  N is at least one additional A and/or C and/or G and/or U

<220>
<221>  misc_feature
<222>  (19)..(19)
<223>  D is A or G or U

<400>  45
naaccccuac caacuggudg ggguuugaaa c                                      31


<210>  46
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DR stem loop

<400>  46
aaccccuacc aacuggucgg gguaugaaac                                        30


<210>  47
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Flag tag

<400>  47

Asp Tyr Lys Asp Asp Asp Lys 
1               5           


<210>  48
<211>  1000
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the Cas 13d variant

<400>  48

Met Ser Pro Lys Lys Lys Arg Lys Val Glu Ala Ser Ile Glu Lys Lys 
1               5                   10                  15      


Lys Ser Phe Ala Lys Gly Met Gly Val Lys Ser Thr Leu Val Ser Gly 
            20                  25                  30          


Ser Lys Val Tyr Met Thr Thr Phe Ala Glu Gly Ser Asp Ala Arg Leu 
        35                  40                  45              


Glu Lys Ile Val Glu Gly Asp Ser Ile Arg Ser Val Asn Glu Gly Glu 
    50                  55                  60                  


Ala Phe Ser Ala Glu Met Ala Asp Lys Asn Ala Gly Tyr Lys Ile Gly 
65                  70                  75                  80  


Asn Ala Lys Phe Ser His Pro Lys Gly Tyr Ala Val Val Ala Asn Asn 
                85                  90                  95      


Pro Leu Tyr Thr Gly Pro Val Gln Gln Asp Met Leu Gly Leu Lys Glu 
            100                 105                 110         


Thr Leu Glu Lys Arg Tyr Phe Gly Glu Ser Ala Asp Gly Asn Asp Asn 
        115                 120                 125             


Ile Cys Ile Gln Val Ile His Asn Ile Leu Asp Ile Glu Lys Ile Leu 
    130                 135                 140                 


Ala Glu Tyr Ile Thr Asn Ala Ala Tyr Ala Val Asn Asn Ile Ser Gly 
145                 150                 155                 160 


Leu Asp Lys Asp Ile Ile Gly Phe Gly Lys Phe Ser Thr Val Tyr Thr 
                165                 170                 175     


Tyr Asp Glu Phe Lys Asp Pro Glu His His Arg Ala Ala Phe Asn Asn 
            180                 185                 190         


Asn Asp Lys Leu Ile Asn Ala Ile Lys Ala Gln Tyr Asp Glu Phe Asp 
        195                 200                 205             


Asn Phe Leu Asp Asn Pro Arg Leu Gly Tyr Phe Gly Gln Ala Phe Phe 
    210                 215                 220                 


Ser Lys Glu Gly Arg Asn Tyr Ile Ile Asn Tyr Gly Asn Glu Cys Tyr 
225                 230                 235                 240 


Asp Ile Leu Ala Leu Leu Ser Gly Leu Arg His Trp Val Val His Asn 
                245                 250                 255     


Asn Glu Glu Glu Ser Arg Ile Ser Arg Thr Trp Leu Tyr Asn Leu Asp 
            260                 265                 270         


Lys Asn Leu Asp Asn Glu Tyr Ile Ser Thr Leu Asn Tyr Leu Tyr Asp 
        275                 280                 285             


Arg Ile Thr Asn Glu Leu Thr Asn Ser Phe Ser Lys Asn Ser Ala Ala 
    290                 295                 300                 


Asn Val Asn Tyr Ile Ala Glu Thr Leu Gly Ile Asn Pro Ala Glu Phe 
305                 310                 315                 320 


Ala Glu Gln Tyr Phe Arg Phe Ser Ile Met Lys Glu Gln Lys Asn Leu 
                325                 330                 335     


Gly Phe Asn Ile Thr Lys Leu Arg Glu Val Met Leu Asp Arg Lys Asp 
            340                 345                 350         


Met Ser Glu Ile Arg Lys Asn His Lys Val Phe Asp Ser Ile Arg Thr 
        355                 360                 365             


Lys Val Tyr Thr Met Met Asp Phe Val Ile Tyr Arg Tyr Tyr Ile Glu 
    370                 375                 380                 


Glu Asp Ala Lys Val Ala Ala Ala Asn Lys Ser Leu Pro Asp Asn Glu 
385                 390                 395                 400 


Lys Ser Leu Ser Glu Lys Asp Ile Phe Val Ile Asn Leu Arg Gly Ser 
                405                 410                 415     


Phe Asn Asp Asp Gln Lys Asp Ala Leu Tyr Tyr Asp Glu Ala Asn Arg 
            420                 425                 430         


Ile Trp Arg Lys Leu Glu Asn Ile Met His Asn Ile Lys Glu Phe Arg 
        435                 440                 445             


Gly Asn Lys Thr Arg Glu Tyr Lys Lys Lys Asp Ala Pro Arg Leu Pro 
    450                 455                 460                 


Arg Ile Leu Pro Ala Gly Arg Asp Val Ser Ala Phe Ser Lys Leu Met 
465                 470                 475                 480 


Tyr Ala Leu Thr Met Phe Leu Asp Gly Lys Glu Ile Asn Asp Leu Leu 
                485                 490                 495     


Thr Thr Leu Ile Asn Lys Phe Asp Asn Ile Gln Ser Phe Leu Lys Val 
            500                 505                 510         


Met Pro Leu Ile Gly Val Asn Ala Lys Phe Val Glu Glu Tyr Ala Phe 
        515                 520                 525             


Phe Lys Asp Ser Ala Lys Ile Ala Asp Glu Leu Arg Leu Ile Lys Ser 
    530                 535                 540                 


Phe Ala Arg Met Gly Glu Pro Ile Ala Asp Ala Arg Arg Ala Met Tyr 
545                 550                 555                 560 


Ile Asp Ala Ile Arg Ile Leu Gly Thr Asn Leu Ser Tyr Asp Glu Leu 
                565                 570                 575     


Lys Ala Leu Ala Asp Thr Phe Ser Leu Asp Glu Asn Gly Asn Lys Leu 
            580                 585                 590         


Lys Lys Gly Lys His Gly Met Arg Asn Phe Ile Ile Asn Asn Val Ile 
        595                 600                 605             


Ser Asn Lys Arg Phe His Tyr Leu Ile Arg Tyr Gly Asp Pro Ala His 
    610                 615                 620                 


Leu His Glu Ile Ala Lys Asn Glu Ala Val Val Lys Phe Val Leu Gly 
625                 630                 635                 640 


Arg Ile Ala Asp Ile Gln Lys Lys Gln Gly Gln Asn Gly Lys Asn Gln 
                645                 650                 655     


Ile Asp Arg Tyr Tyr Glu Thr Cys Ile Gly Lys Asp Lys Gly Lys Ser 
            660                 665                 670         


Val Ser Glu Lys Val Asp Ala Leu Thr Lys Ile Ile Thr Gly Met Asn 
        675                 680                 685             


Tyr Asp Gln Phe Asp Lys Lys Arg Ser Val Ile Glu Asp Thr Gly Arg 
    690                 695                 700                 


Glu Asn Ala Glu Arg Glu Lys Phe Lys Lys Ile Ile Ser Leu Tyr Leu 
705                 710                 715                 720 


Thr Val Ile Tyr His Ile Leu Lys Asn Ile Val Asn Ile Asn Ala Arg 
                725                 730                 735     


Tyr Val Ile Gly Phe His Cys Val Glu Arg Asp Ala Gln Leu Tyr Lys 
            740                 745                 750         


Glu Lys Gly Tyr Asp Ile Asn Leu Lys Lys Leu Glu Glu Lys Gly Phe 
        755                 760                 765             


Ser Ser Val Thr Lys Leu Cys Ala Gly Ile Asp Glu Thr Ala Pro Asp 
    770                 775                 780                 


Lys Arg Lys Asp Val Glu Lys Glu Met Ala Glu Arg Ala Lys Glu Ser 
785                 790                 795                 800 


Ile Asp Ser Leu Glu Ser Ala Asn Pro Lys Leu Tyr Ala Asn Tyr Ile 
                805                 810                 815     


Lys Tyr Ser Asp Glu Lys Lys Ala Glu Glu Phe Thr Arg Gln Ile Asn 
            820                 825                 830         


Arg Glu Lys Ala Lys Thr Ala Leu Asn Ala Tyr Leu Arg Asn Thr Lys 
        835                 840                 845             


Trp Asn Val Ile Ile Arg Glu Asp Leu Leu Arg Ile Asp Asn Lys Thr 
    850                 855                 860                 


Cys Thr Leu Phe Arg Asn Lys Ala Val His Leu Glu Val Ala Arg Tyr 
865                 870                 875                 880 


Val His Ala Tyr Ile Asn Asp Ile Ala Glu Val Asn Ser Tyr Phe Gln 
                885                 890                 895     


Leu Tyr His Tyr Ile Met Gln Arg Ile Ile Met Asn Glu Arg Tyr Glu 
            900                 905                 910         


Lys Ser Ser Gly Lys Val Ser Glu Tyr Phe Asp Ala Val Asn Asp Glu 
        915                 920                 925             


Lys Lys Tyr Asn Asp Arg Leu Leu Lys Leu Leu Cys Val Pro Phe Gly 
    930                 935                 940                 


Tyr Cys Ile Pro Arg Phe Lys Asn Leu Ser Ile Glu Ala Leu Phe Asp 
945                 950                 955                 960 


Arg Asn Glu Ala Ala Lys Phe Asp Lys Glu Lys Lys Lys Val Ser Gly 
                965                 970                 975     


Asn Ser Gly Ser Gly Pro Lys Lys Lys Arg Lys Val Ala Ala Ala Tyr 
            980                 985                 990         


Pro Tyr Asp Val Pro Asp Tyr Ala  
        995                 1000 


